CRISPOR

DNA break repair outcome predictions

Guide: CCTCTGTGCCGCACCGCCTC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CACCTTGTGATGCGAGCGCTCCTCTGTGCCGCACCGCCTCTGGGAGGAAGCCCCATTGCCCTCTTCTTTCTAAGCTGTCA`	(Wild-type sequence)
298	`TGCGAGCGCTCCTCTGTGCCGCACCGCC--------------CCATTGCCCTCTTCTTTC`	`14 bp deleted → frameshift`
221	`TGCGAGCGCTCCTCTGTGCCGCACCGCCTC-----------------------TTCTTTC`	`23 bp deleted → frameshift`
199	`TGCGAGCGCTCCTCTGTGCCGCACCGCC----------------------CTCTTCTTTC`	`22 bp deleted → frameshift`
199	`TGCGAGCGCTCCTCTGTGCC----------------------CCATTGCCCTCTTCTTTC`	`22 bp deleted → frameshift`
188	`TGCGAGCGCTCCTCTGTGCCGCACCGCC---------------CATTGCCCTCTTCTTTC`	`15 bp deleted → no frameshift`
179	`TGCGAGCGCTCCTCTGTGCCGCACCGCC----------------ATTGCCCTCTTCTTTC`	`16 bp deleted → frameshift`
170	`TGCGAGCGCTCCTCTGTGCCGCACC-----------------CCATTGCCCTCTTCTTTC`	`17 bp deleted → frameshift`
162	`TGCGAGCGCTCCTCTGTGCCGCACC------------------CATTGCCCTCTTCTTTC`	`18 bp deleted → no frameshift`
156	`TGCGAGCGCTCCTCTGTGCC------------------------------CTCTTCTTTC`	`30 bp deleted → no frameshift`
154	`TGCGAGCGCTCCTCTGTGCCGCACC-------------------ATTGCCCTCTTCTTTC`	`19 bp deleted → frameshift`
154	`TGCGAGCGCTCCTCTGTGCCGC-------------------CCCATTGCCCTCTTCTTTC`	`19 bp deleted → frameshift`
149	`TGCGAGCGCTCCTCTGTG--------------GGAGGAAGCCCCATTGCCCTCTTCTTTC`	`14 bp deleted → frameshift`
134	`TGCGAGCGCTCCTCTG----------------GGAGGAAGCCCCATTGCCCTCTTCTTTC`	`16 bp deleted → frameshift`
126	`TGCGAGCGCTCCTCTGTGCC-----------------------CATTGCCCTCTTCTTTC`	`23 bp deleted → frameshift`
120	`TGCGAGCGCTCCTCTGTGCC------------------------ATTGCCCTCTTCTTTC`	`24 bp deleted → no frameshift`
114	`TGCGAGCGCTCCTCTGTGCCGCACC-------------------------CTCTTCTTTC`	`25 bp deleted → frameshift`
113	`TGCGAGCGCTCCTCT---------------------------------------TCTTTC`	`39 bp deleted → no frameshift`
111	`TGCGAG------------------------------GAAGCCCCATTGCCCTCTTCTTTC`	`30 bp deleted → no frameshift`
109	`TGCGAGCGCTCCTCTGTGCCGCACC--------------------------TCTTCTTTC`	`26 bp deleted → frameshift`
103	`TGCGAGCGCTCCTCTGTGCCGC---------------------------CCTCTTCTTTC`	`27 bp deleted → no frameshift`
99	`TGCGAGCGCTCCTCTGTGCCGCA----------------------TTGCCCTCTTCTTTC`	`22 bp deleted → frameshift`
91	`TGCGAGC----------------------------------CCCATTGCCCTCTTCTTTC`	`34 bp deleted → frameshift`
89	`TGCGAGCGCTCC------------------------------CCATTGCCCTCTTCTTTC`	`30 bp deleted → no frameshift`
86	`TGCGAGCGCTCCTCTGTGCCGCACCGCCT-------------------------TCTTTC`	`25 bp deleted → frameshift`
84	`TGCGAGCGCTCCTCTGTGCC-------------------------------TCTTCTTTC`	`31 bp deleted → frameshift`
84	`TGCGAGCGCTCC-------------------------------CATTGCCCTCTTCTTTC`	`31 bp deleted → frameshift`
81	`TGCGAGCGCTCCTCTGTGCCGCACCGCCTC--------------------------TTTC`	`26 bp deleted → frameshift`
80	`TGCGAGCGCTCC--------------------------------ATTGCCCTCTTCTTTC`	`32 bp deleted → frameshift`
80	`TGCGAGCGC--------------------------------CCCATTGCCCTCTTCTTTC`	`32 bp deleted → frameshift`
74	`TGCGAGCGCTCCTCTGTGCCGCACCGCCT----------------------------TTC`	`28 bp deleted → frameshift`
66	`TGCGAGCGCTCCTCTGTGCCGCACCGCCTC------------------------------`	`30 bp deleted → no frameshift`
66	`TG------------------------------GGAGGAAGCCCCATTGCCCTCTTCTTTC`	`30 bp deleted → no frameshift`
61	`TGCGAGCGCTC------------------------------------------TTCTTTC`	`42 bp deleted → no frameshift`
60	`TGCGAGCGCTCCTCTG--------------------------------CCCTCTTCTTTC`	`32 bp deleted → frameshift`
60	`TGCGAGCGCTCC--------------------------------------CTCTTCTTTC`	`38 bp deleted → frameshift`
60	`TGC--------------------------------------CCCATTGCCCTCTTCTTTC`	`38 bp deleted → frameshift`
57	`TGCGA---------------------------------AGCCCCATTGCCCTCTTCTTTC`	`33 bp deleted → no frameshift`
54	`TGCGAGCGC----------------------------------------CCTCTTCTTTC`	`40 bp deleted → frameshift`
50	`TGC----------------------------------------------CCTCTTCTTTC`	`46 bp deleted → frameshift`
48	`TGCGAGCGCTCCTCT------------------------------------------TTC`	`42 bp deleted → no frameshift`
48	`TGCGAGC------------------------------------------CCTCTTCTTTC`	`42 bp deleted → no frameshift`
47	`TGCGAGCGCTCCTCT-------------------------------------CTTCTTTC`	`37 bp deleted → frameshift`
38	`TGCGAGCGCTCCT-----------------------------------------TCTTTC`	`41 bp deleted → frameshift`
33	`TGCGAGCGCTCCT--------------------------------------------TTC`	`44 bp deleted → frameshift`
33	`TGCGAGCGCT--------------------------------------------TCTTTC`	`44 bp deleted → frameshift`
31	`TGCGAGCGCTC---------------------------------------------TTTC`	`45 bp deleted → no frameshift`
30	`TGCGAGCGCTCCTC----------------------------------------------`	`46 bp deleted → frameshift`
28	`TGCGAGCGCT-----------------------------------------------TTC`	`47 bp deleted → frameshift`
25	`TGCGAGCGCTC-------------------------------------------------`	`49 bp deleted → frameshift`

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