CRISPOR

DNA break repair outcome predictions

Guide: CAGAGAGGCTGTCCGCGGAG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCTACTAGGTTGCCCTTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCATGGCCGCTCCAGCAG`	(Wild-type sequence)
389	`TGCCCTTGGCCAGAGAGGCTGTCCGCGGA-----CTGGGGGTCCCGGGCCAAGCATGGCC`	`5 bp deleted → frameshift`
327	`TGCCCTTGGCCAGAGAGGCTGTCCGCGG----GACTGGGGGTCCCGGGCCAAGCATGGCC`	`4 bp deleted → frameshift`
257	`TGCCCTTGGCCAGAGAGGCTGTCC--------------------CGGGCCAAGCATGGCC`	`20 bp deleted → frameshift`
242	`TGCCCTTGGCCAGAGAGGCTGTCCGCGG----------GGGTCCCGGGCCAAGCATGGCC`	`10 bp deleted → frameshift`
232	`TGCCCTTGGCCAGAGAGGCTGTCCGCGG-------------------GCCAAGCATGGCC`	`19 bp deleted → frameshift`
230	`TGCCCTTGGCCAGAGAGGCTGTCCGCGG-----------GGTCCCGGGCCAAGCATGGCC`	`11 bp deleted → frameshift`
224	`TGCCCTTGGCCAGAGAGGCTG----------------GGGGTCCCGGGCCAAGCATGGCC`	`16 bp deleted → frameshift`
219	`TGCCCTTGGCCAGAGAGGCTGTCCGCGG------------GTCCCGGGCCAAGCATGGCC`	`12 bp deleted → no frameshift`
210	`TGCCCTTGGCCAGAGAGGCTGTCCG---------------------GGCCAAGCATGGCC`	`21 bp deleted → no frameshift`
208	`TGCCCTTGGCCAGAGAGGCTGTCCGCGG-------------TCCCGGGCCAAGCATGGCC`	`13 bp deleted → frameshift`
198	`TGCCCTTGGCCAGAGAGG--------------GACTGGGGGTCCCGGGCCAAGCATGGCC`	`14 bp deleted → frameshift`
188	`TGCCCTTGGCCAGAGAGG---------------ACTGGGGGTCCCGGGCCAAGCATGGCC`	`15 bp deleted → no frameshift`
147	`TGCCCTTGGCCAGAGAGGCTGTCCGCGG--------------------CCAAGCATGGCC`	`20 bp deleted → frameshift`
147	`TGCCCTTGGCCAGAGAGG--------------------GGGTCCCGGGCCAAGCATGGCC`	`20 bp deleted → frameshift`
140	`TGCCCTTGGCCAGAGAGG---------------------GGTCCCGGGCCAAGCATGGCC`	`21 bp deleted → no frameshift`
133	`TGCCCTTGGCCAGAGAGGC------------------------------CAAGCATGGCC`	`30 bp deleted → no frameshift`
133	`TGCCCTTGGCCAGAGAGG----------------------GTCCCGGGCCAAGCATGGCC`	`22 bp deleted → frameshift`
127	`TGCCCTTGGCCA---------------------------------------AGCATGGCC`	`39 bp deleted → no frameshift`
126	`TGCCCTTGGCCAGAGAGGCTGTCCGC-----------------------CAAGCATGGCC`	`23 bp deleted → frameshift`
126	`TGCCCTTGGCCAGAGAGG-----------------------TCCCGGGCCAAGCATGGCC`	`23 bp deleted → frameshift`
126	`TGCCCTTGG-----------------------GACTGGGGGTCCCGGGCCAAGCATGGCC`	`23 bp deleted → frameshift`
122	`TGCCCTTGGCCAGAGA------------------CTGGGGGTCCCGGGCCAAGCATGGCC`	`18 bp deleted → no frameshift`
120	`TGCCCTTGG------------------------ACTGGGGGTCCCGGGCCAAGCATGGCC`	`24 bp deleted → no frameshift`
117	`TGCCCTTGG-----------------------------GGGTCCCGGGCCAAGCATGGCC`	`29 bp deleted → frameshift`
110	`TGCCCTTGGCCAGA--------------------CTGGGGGTCCCGGGCCAAGCATGGCC`	`20 bp deleted → frameshift`
109	`TGCCCTTGGCCAGAGAGGCTGTCC--------------------------AAGCATGGCC`	`26 bp deleted → frameshift`
98	`TGCCCTTGGCCAGAGAGGCTGTCCGC----------------------------ATGGCC`	`28 bp deleted → frameshift`
95	`TGCCCTTGGCCAGAGAGGCTGTCCGCGGAG-----------------------CATGGCC`	`23 bp deleted → frameshift`
94	`TGCCCTTGGCCAGAGAGG-----------------------------GCCAAGCATGGCC`	`29 bp deleted → frameshift`
89	`TGCCCTTGGCCAGAGAGGCTGTCCGCGG------------------------------CC`	`30 bp deleted → no frameshift`
89	`TGCCCTTGG------------------------------GGTCCCGGGCCAAGCATGGCC`	`30 bp deleted → no frameshift`
84	`TGCCCTTGG-------------------------------GTCCCGGGCCAAGCATGGCC`	`31 bp deleted → frameshift`
81	`TGCCCTTGGCCAGAGAGGC----------------------------------------C`	`40 bp deleted → frameshift`
81	`TGCCC----------------------------------------GGGCCAAGCATGGCC`	`40 bp deleted → frameshift`
80	`TGCCCTTGG--------------------------------TCCCGGGCCAAGCATGGCC`	`32 bp deleted → frameshift`
77	`TGCCCTTGGCC-------------------------------------------------`	`49 bp deleted → frameshift`
76	`TGCCCTTGGCCAGAGAGGCTGTCCGC---------------------------------C`	`33 bp deleted → no frameshift`
76	`TGCCCTTGGCC---------------------------------CGGGCCAAGCATGGCC`	`33 bp deleted → no frameshift`
73	`TGCCCTTGGCC----------------------------------GGGCCAAGCATGGCC`	`34 bp deleted → frameshift`
69	`TGCCCTTGGCCAGAGAGGC-----------------------------------ATGGCC`	`35 bp deleted → frameshift`
66	`TGCCCT------------------------------GGGGGTCCCGGGCCAAGCATGGCC`	`30 bp deleted → no frameshift`
66	`TGCCCTTGGCCAGAGAGGCTGTCC------------------------------------`	`36 bp deleted → no frameshift`
60	`TGCCCTTGG--------------------------------------GCCAAGCATGGCC`	`38 bp deleted → frameshift`
60	`TGCC----------------------------------------------AAGCATGGCC`	`46 bp deleted → frameshift`
56	`TGCCC---------------------------------------CGGGCCAAGCATGGCC`	`39 bp deleted → no frameshift`
52	`TG-----------------------------------GGGGTCCCGGGCCAAGCATGGCC`	`35 bp deleted → frameshift`
51	`TGCC-----------------------------------------GGGCCAAGCATGGCC`	`41 bp deleted → frameshift`
49	`TGCCCTTGGCCAGAGAGGCTG------------------------------------GCC`	`36 bp deleted → no frameshift`
49	`TGCCCTTGGCCAGAGAG------------------------------------CATGGCC`	`36 bp deleted → no frameshift`
45	`TGCCCTTGGCCAGAG--------------------------------------CATGGCC`	`38 bp deleted → frameshift`
44	`TGCCCTTGGC--------------------------------------------ATGGCC`	`44 bp deleted → frameshift`
42	`TGCCC---------------------------------------------AAGCATGGCC`	`45 bp deleted → no frameshift`
40	`TGCCCTTGGCCAG----------------------------------------CATGGCC`	`40 bp deleted → frameshift`
36	`TGCC--------------------------------------------------------`	`56 bp deleted → frameshift`
34	`TGCCCTTGGCCA-------------------------------------------TGGCC`	`43 bp deleted → frameshift`
31	`TGC---------------------------------------------------ATGGCC`	`51 bp deleted → no frameshift`
25	`TGCCC-------------------------------------------------------`	`55 bp deleted → frameshift`
19	`TG-------------------------------------------------------GCC`	`55 bp deleted → frameshift`

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