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Contents:

PCR primers for off-targets of ACATGGTTATGTTTCTGGAT GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313860_F Primer3: not found at this Tm N.d. 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313860_R Primer3: not found at this Tm N.d. 1.00
mm4_intron_Gstcd_chr3_133084217_F Primer3: not found at this Tm N.d. 0.67
mm4_intron_Gstcd_chr3_133084217_R Primer3: not found at this Tm N.d. 0.67
mm4_intergenic_Igkv9-123|Igkv1-122_chr6_67998285_F TCGTCGGCAGCGTCAACAAGGTTTCTTTCATTTGTGTTT 57.0 0.56
mm4_intergenic_Igkv9-123|Igkv1-122_chr6_67998285_R GTCTCGTGGGCTCGGTCACATCCAAAACCTAACAGTTTGT 59.3 0.56
mm4_intergenic_Foxf2|Gm25186_chr13_31638346_F Primer3: not found at this Tm N.d. 0.50
mm4_intergenic_Foxf2|Gm25186_chr13_31638346_R Primer3: not found at this Tm N.d. 0.50
mm4_intergenic_Xkr4|Gm1992_chr1_3454982_F TCGTCGGCAGCGTCCCCACATTGGTGATGTCCCA 59.9 0.48
mm4_intergenic_Xkr4|Gm1992_chr1_3454982_R GTCTCGTGGGCTCGGCCACTGAAAGGAGACGTGGT 59.6 0.48
mm4_intergenic_Nek7|Lhx9_chr1_138796465_F Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_Nek7|Lhx9_chr1_138796465_R Primer3: not found at this Tm N.d. 0.46
mm3_intergenic_Gm5468|Myo10_chr15_25457149_F Primer3: not found at this Tm N.d. 0.45
mm3_intergenic_Gm5468|Myo10_chr15_25457149_R Primer3: not found at this Tm N.d. 0.45
mm4_intergenic_Scn9a|Scn7a_chr2_66670497_F Primer3: not found at this Tm N.d. 0.45
mm4_intergenic_Scn9a|Scn7a_chr2_66670497_R Primer3: not found at this Tm N.d. 0.45
mm4_intergenic_mmu-mir-6342|Gm23771_chr1_29914213_F TCGTCGGCAGCGTCTGTTCAGTCTGCAGATTGAGGA 59.3 0.44
mm4_intergenic_mmu-mir-6342|Gm23771_chr1_29914213_R GTCTCGTGGGCTCGGAAACAGTTGAGGTACCCAACA 57.3 0.44
mm4_intergenic_Slitrk5|Gm22764_chr14_111725404_F Primer3: not found at this Tm N.d. 0.42
mm4_intergenic_Slitrk5|Gm22764_chr14_111725404_R Primer3: not found at this Tm N.d. 0.42
mm3_intergenic_Prdm4|Ascl4_chr10_85917791_F TCGTCGGCAGCGTCGGCAATATCCCATACCCCACA 59.5 0.38
mm3_intergenic_Prdm4|Ascl4_chr10_85917791_R GTCTCGTGGGCTCGGACAGATCATTAACAAAGTTGCAAGG 58.5 0.38
mm4_intron_Rapgef5_chr12_117578034_F Primer3: not found at this Tm N.d. 0.38
mm4_intron_Rapgef5_chr12_117578034_R Primer3: not found at this Tm N.d. 0.38
mm4_intron_Pde4d_chr13_109570572_F Primer3: not found at this Tm N.d. 0.37
mm4_intron_Pde4d_chr13_109570572_R Primer3: not found at this Tm N.d. 0.37
mm4_intergenic_Mrgprb2|Mrgprb3_chr7_48591242_F Primer3: not found at this Tm N.d. 0.37
mm4_intergenic_Mrgprb2|Mrgprb3_chr7_48591242_R Primer3: not found at this Tm N.d. 0.37
mm4_intron_Cnga2_chrX_71993671_F TCGTCGGCAGCGTCAGGATGGATGGGGAAGAGGA 59.3 0.36
mm4_intron_Cnga2_chrX_71993671_R GTCTCGTGGGCTCGGGCTCATCTTTCCTCACAAGCC 59.2 0.36
mm3_intergenic_Gm26076|Gm25519_chr3_111172871_F Primer3: not found at this Tm N.d. 0.35
mm3_intergenic_Gm26076|Gm25519_chr3_111172871_R Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Zfyve16|Spz1_chr13_92546155_F Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Zfyve16|Spz1_chr13_92546155_R Primer3: not found at this Tm N.d. 0.35
mm4_intron_Nrxn3_chr12_89135270_F Primer3: not found at this Tm N.d. 0.32
mm4_intron_Nrxn3_chr12_89135270_R Primer3: not found at this Tm N.d. 0.32
mm4_intergenic_Agmo|Dgkb_chr12_37675848_F Primer3: not found at this Tm N.d. 0.32
mm4_intergenic_Agmo|Dgkb_chr12_37675848_R Primer3: not found at this Tm N.d. 0.32
mm4_intergenic_Gm4654|Scel_chr14_103392070_F Primer3: not found at this Tm N.d. 0.32
mm4_intergenic_Gm4654|Scel_chr14_103392070_R Primer3: not found at this Tm N.d. 0.32
mm3_intergenic_1810046K07Rik|Gm7293_chr9_51439352_F Primer3: not found at this Tm N.d. 0.30
mm3_intergenic_1810046K07Rik|Gm7293_chr9_51439352_R Primer3: not found at this Tm N.d. 0.30
mm4_intron_Crb1_chr1_139324977_F Primer3: not found at this Tm N.d. 0.29
mm4_intron_Crb1_chr1_139324977_R Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Gm23424|Gm6756_chr18_36877907_F Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Gm23424|Gm6756_chr18_36877907_R Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Gm833|Gm25779_chr4_152878411_F TCGTCGGCAGCGTCACACACACAAAGCTCCCTCA 59.4 0.28
mm4_intergenic_Gm833|Gm25779_chr4_152878411_R GTCTCGTGGGCTCGGCGGAAGTGTCTCTGGAGTCC 59.4 0.28
mm2_intergenic_Gm13468|Gm13481_chr2_48004953_F TCGTCGGCAGCGTCGCGGGACAAGAGGTTAAAATGG 59.8 0.28
mm2_intergenic_Gm13468|Gm13481_chr2_48004953_R GTCTCGTGGGCTCGGTCACAGGGATTGCATCAGTT 57.1 0.28
mm4_intergenic_Gm23558|Galc_chr12_97974206_F TCGTCGGCAGCGTCACGAATGCAACGGTAGTTTGC 60.0 0.28
mm4_intergenic_Gm23558|Galc_chr12_97974206_R GTCTCGTGGGCTCGGAGGACACAAATGCTCCCCAG 59.9 0.28
mm4_intergenic_Psg29|Gm24520_chr7_17528519_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Psg29|Gm24520_chr7_17528519_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm13577|Gm13576_chr2_64498523_F Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Gm13577|Gm13576_chr2_64498523_R Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Gm22939|Cntnap2_chr6_45037011_F Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Gm22939|Cntnap2_chr6_45037011_R Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Lzts3|Ddrgk1_chr2_130651698_F TCGTCGGCAGCGTCAGATTTGCTTGAGTTACATGAGAG 57.0 0.25
mm4_intergenic_Lzts3|Ddrgk1_chr2_130651698_R GTCTCGTGGGCTCGGCTCAGGGCTGTGGATATAGCA 59.0 0.25
mm4_intron_Tmem163_chr1_127596556_F TCGTCGGCAGCGTCCATCCACCGGTTCAGATGCT 60.1 0.24
mm4_intron_Tmem163_chr1_127596556_R GTCTCGTGGGCTCGGTGTAGCACATTCCATCTTAGCT 57.2 0.24
mm4_intron_Kcnmb4_chr10_116446942_F Primer3: not found at this Tm N.d. 0.24
mm4_intron_Kcnmb4_chr10_116446942_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm6806|Gm22332_chrX_68545508_F TCGTCGGCAGCGTCACTTTAATCCCTTCTGAGAGGAGAC 59.5 0.23
mm4_intergenic_Gm6806|Gm22332_chrX_68545508_R GTCTCGTGGGCTCGGACAGGTTGCTTACAAGATTTCATGT 59.4 0.23
mm4_intergenic_A530020G20Rik|A730020M07Rik_chr3_121624291_F Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_A530020G20Rik|A730020M07Rik_chr3_121624291_R Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm22483|Prelid2_chr18_41099431_F TCGTCGGCAGCGTCTCCCATAGGAATTCAAGACAAGCT 59.7 0.22
mm4_intergenic_Gm22483|Prelid2_chr18_41099431_R GTCTCGTGGGCTCGGTGCTGAGTATCTACTGCCTCTCT 60.1 0.22
mm4_intergenic_4833403I15Rik|Arl14epl_chr18_46916547_F TCGTCGGCAGCGTCCTGATGGGCTGATTGATGAGGA 59.8 0.22
mm4_intergenic_4833403I15Rik|Arl14epl_chr18_46916547_R GTCTCGTGGGCTCGGACCTACAAGCACCACACACT 59.1 0.22
mm4_intron_Arhgap29_chr3_122001886_F TCGTCGGCAGCGTCGCTCTTTCTCACGTGGCTCT 60.0 0.21
mm4_intron_Arhgap29_chr3_122001886_R GTCTCGTGGGCTCGGTGCTATCCTATCCCCTGCTT 57.5 0.21
mm4_intergenic_Zfp846|5730577I03Rik_chr9_20597947_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Zfp846|5730577I03Rik_chr9_20597947_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Pax7_chr4_139827794_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Pax7_chr4_139827794_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Egfem1_chr3_29355099_F Primer3: not found at this Tm N.d. 0.20
mm4_intron_Egfem1_chr3_29355099_R Primer3: not found at this Tm N.d. 0.20
mm4_intron_Arhgap15_chr2_44384697_F TCGTCGGCAGCGTCTCTCTGGTAACTCACTGATACTCT 57.9 0.20
mm4_intron_Arhgap15_chr2_44384697_R GTCTCGTGGGCTCGGTCCAAGACATCCCGCTTCAC 60.0 0.20
mm4_intergenic_Auts2|Gm25722_chr5_132784948_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Auts2|Gm25722_chr5_132784948_R Primer3: not found at this Tm N.d. 0.20
mm4_intron_MDGA2/Mdga2_chr12_66534565_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_MDGA2/Mdga2_chr12_66534565_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Wdr49|Pdcd10_chr3_75508738_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Wdr49|Pdcd10_chr3_75508738_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gli3|Inhba_chr13_15767252_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gli3|Inhba_chr13_15767252_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Cyp4f14_chr17_32908252_F Primer3: not found at this Tm N.d. 0.18
mm4_intron_Cyp4f14_chr17_32908252_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Prkg1_chr19_30857736_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Prkg1_chr19_30857736_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Dclk3_chr9_111451032_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Dclk3_chr9_111451032_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm8609|Gm22543_chr16_40102236_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm8609|Gm22543_chr16_40102236_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Eya1|Msc_chr1_14602398_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Eya1|Msc_chr1_14602398_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Myc|Pvt1/PVT1_1_chr15_61991568_F TCGTCGGCAGCGTCGACACACGCTCATCAATGGC 59.9 0.17
mm4_intergenic_Myc|Pvt1/PVT1_1_chr15_61991568_R GTCTCGTGGGCTCGGGGTACCTTTCTCCCTGGACT 58.0 0.17
mm4_intron_Slco1a6_chr6_142088626_F TCGTCGGCAGCGTCACATCCCCTGCCATAAACCC 59.7 0.17
mm4_intron_Slco1a6_chr6_142088626_R GTCTCGTGGGCTCGGTTCAGCCTAAAGGAATTTGCAC 57.4 0.17
mm4_intergenic_Gm14569|Pgrmc1_chrX_36509801_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm14569|Pgrmc1_chrX_36509801_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Utp20|Slc5a8_chr10_88850381_F TCGTCGGCAGCGTCTTTACCGTAAGACGCAGGCG 60.7 0.16
mm4_intergenic_Utp20|Slc5a8_chr10_88850381_R GTCTCGTGGGCTCGGAAGCTCAGCCAGTCTTGCTT 59.8 0.16
mm4_intergenic_Gm5899|Gm23884_chr7_94543671_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm5899|Gm23884_chr7_94543671_R Primer3: not found at this Tm N.d. 0.16
mm4_intron_Cluh_chr11_74651415_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_Cluh_chr11_74651415_R Primer3: not found at this Tm N.d. 0.16
mm4_intron_Dscam_chr16_96748749_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Dscam_chr16_96748749_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Dus1l|Fasn_chr11_120802190_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Dus1l|Fasn_chr11_120802190_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Cdh6|Gm9630_chr15_13521559_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Cdh6|Gm9630_chr15_13521559_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm23131|Lphn2_chr3_148602649_F TCGTCGGCAGCGTCCCCAACCCAAACACAAGCAG 59.8 0.15
mm4_intergenic_Gm23131|Lphn2_chr3_148602649_R GTCTCGTGGGCTCGGGCAACTCACAGTGACTAGGTCT 59.7 0.15
mm4_intergenic_Gm16542|Fhit_chr14_10963152_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm16542|Fhit_chr14_10963152_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm24250|Nlgn1_chr3_25849721_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm24250|Nlgn1_chr3_25849721_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Mageb1|Gm4911_chrX_92040798_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Mageb1|Gm4911_chrX_92040798_R Primer3: not found at this Tm N.d. 0.14
mm3_intergenic_Gm4884|Gm2128_chr7_41097014_F TCGTCGGCAGCGTCAGAGGTGACCCATTGACAACA 59.2 0.14
mm3_intergenic_Gm4884|Gm2128_chr7_41097014_R GTCTCGTGGGCTCGGCTTCCAGCACTGTATCTCACA 57.4 0.14
mm4_intergenic_Ralgps2|Tex35_chr1_157041581_F TCGTCGGCAGCGTCTGGAGAGGCGGTGAATTCAG 59.7 0.14
mm4_intergenic_Ralgps2|Tex35_chr1_157041581_R GTCTCGTGGGCTCGGGGCTTGGGAAAGGAGGAGAA 59.3 0.14
mm4_intron_Ksr2_chr5_117611521_F TCGTCGGCAGCGTCGGCTCCCCACAAAGGACTTT 60.1 0.14
mm4_intron_Ksr2_chr5_117611521_R GTCTCGTGGGCTCGGGGTTCCTCTTGCCTCTGTCC 60.0 0.14
mm4_intergenic_Gm4922|Perp_chr10_18842007_F TCGTCGGCAGCGTCCCTCCCACAGGTTGTGTAGAC 59.9 0.14
mm4_intergenic_Gm4922|Perp_chr10_18842007_R GTCTCGTGGGCTCGGTGACACAACAGCCACTTCCG 60.8 0.14
mm4_intergenic_Gm23891|1700018B24Rik_chr3_48588075_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm23891|1700018B24Rik_chr3_48588075_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_AV039307|Ubr1/AV039307_chr2_120856549_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_AV039307|Ubr1/AV039307_chr2_120856549_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Rps3a1|Gm23025_chr3_86171509_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Rps3a1|Gm23025_chr3_86171509_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_3100003L05Rik|Gm21957_chr7_125057585_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_3100003L05Rik|Gm21957_chr7_125057585_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Galnt18_chr7_111706832_F TCGTCGGCAGCGTCCCTAATTAATAGGGTCAGTGTCCT 57.2 0.12
mm4_intron_Galnt18_chr7_111706832_R GTCTCGTGGGCTCGGTAAAGAGGCCAGAGCACGTG 60.0 0.12
mm4_exon_Itpr1_chr6_108550912_F Primer3: not found at this Tm N.d. 0.12
mm4_exon_Itpr1_chr6_108550912_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Spink12|Gm10267_chr18_44145779_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Spink12|Gm10267_chr18_44145779_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Cdhr3|F730043M19Rik/Gm16267_chr12_33098195_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Cdhr3|F730043M19Rik/Gm16267_chr12_33098195_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm14672|Gm14668_chrX_65567409_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm14672|Gm14668_chrX_65567409_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm26063|Gm23562_chr15_46327149_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm26063|Gm23562_chr15_46327149_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Kctd16_chr18_40329640_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Kctd16_chr18_40329640_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm22782|Rpl10l_chr12_65711813_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm22782|Rpl10l_chr12_65711813_R Primer3: not found at this Tm N.d. 0.11
mm3_intron_Erbb4_chr1_68264801_F Primer3: not found at this Tm N.d. 0.11
mm3_intron_Erbb4_chr1_68264801_R Primer3: not found at this Tm N.d. 0.11
mm3_intergenic_Chrne|Gm12315_chr11_70620575_F TCGTCGGCAGCGTCAGCCCTTTATGCGTTACACCT 59.7 0.11
mm3_intergenic_Chrne|Gm12315_chr11_70620575_R GTCTCGTGGGCTCGGAGTGACCCTCCGCTACAGAT 60.0 0.11
mm4_intergenic_Fbxo15|Gm25453_chr18_85993773_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Fbxo15|Gm25453_chr18_85993773_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Slc13a4_chr6_35286374_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_Slc13a4_chr6_35286374_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_4933406K04Rik_chr12_106700478_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_4933406K04Rik_chr12_106700478_R Primer3: not found at this Tm N.d. 0.10
mm3_intergenic_Fam173b|Tas2r119_chr15_32119260_F TCGTCGGCAGCGTCGAGCCGCTGTCTGAGAAGTT 60.0 0.10
mm3_intergenic_Fam173b|Tas2r119_chr15_32119260_R GTCTCGTGGGCTCGGGCTCCACCATGCTTCAGAGT 60.0 0.10
mm4_intergenic_Gm24681|Gm23591_chr16_68628019_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm24681|Gm23591_chr16_68628019_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Plp1|Rab9b_chrX_136843601_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Plp1|Rab9b_chrX_136843601_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Triml1|Triml2_chr8_43147636_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Triml1|Triml2_chr8_43147636_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Fbxw11|Gm8192_chr11_32753910_F TCGTCGGCAGCGTCGGTGCACACCCTATGACTTCA 59.9 0.09
mm4_intergenic_Fbxw11|Gm8192_chr11_32753910_R GTCTCGTGGGCTCGGGCCTTGTGCTCCACCTCTAA 59.6 0.09
mm4_intergenic_Gm22223|Gm22715_chr8_102396494_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm22223|Gm22715_chr8_102396494_R Primer3: not found at this Tm N.d. 0.09
mm3_intergenic_Adra2a|Gpam_chr19_54412906_F TCGTCGGCAGCGTCCACTTGGCCCTCGATGAAGT 60.0 0.09
mm3_intergenic_Adra2a|Gpam_chr19_54412906_R GTCTCGTGGGCTCGGTCACCCTAGCTTAGCCACCT 59.9 0.09
mm4_intergenic_Gm26871|Dpyd_chr3_118541161_F TCGTCGGCAGCGTCTCAGTGTTGGGATGGATGGT 58.6 0.09
mm4_intergenic_Gm26871|Dpyd_chr3_118541161_R GTCTCGTGGGCTCGGAGTCTGTTTTGAAGCTTCCTCCA 60.1 0.09
mm4_intergenic_Gm13723|Olfr1097_chr2_86881970_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm13723|Olfr1097_chr2_86881970_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Dok5|Gm14263_chr2_170912170_F TCGTCGGCAGCGTCTCTCAAAGTCAAAATGAAAGTTGGA 57.5 0.09
mm4_intergenic_Dok5|Gm14263_chr2_170912170_R GTCTCGTGGGCTCGGAGAGCCCTGAACCCAATGTG 59.9 0.09
mm4_intergenic_Lrp12|9330182O14Rik_chr15_40083444_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Lrp12|9330182O14Rik_chr15_40083444_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Col23a1_chr11_51527207_F TCGTCGGCAGCGTCGGGATGCAAAACGGGAAGAC 59.4 0.08
mm4_intron_Col23a1_chr11_51527207_R GTCTCGTGGGCTCGGCTACCCCTTCAAGCCCCTTG 60.0 0.08
mm4_intergenic_Naa15|Rab33b_chr3_51479683_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Naa15|Rab33b_chr3_51479683_R Primer3: not found at this Tm N.d. 0.08
mm3_intergenic_6330403L08Rik/Pdgfa|Prkar1b_chr5_139011247_F Primer3: not found at this Tm N.d. 0.08
mm3_intergenic_6330403L08Rik/Pdgfa|Prkar1b_chr5_139011247_R Primer3: not found at this Tm N.d. 0.08
mm4_exon_Hdac11_chr6_91174675_F TCGTCGGCAGCGTCTCCAGGTTGTTGCTGTGTGT 60.0 0.08
mm4_exon_Hdac11_chr6_91174675_R GTCTCGTGGGCTCGGACAACCACTCATCTTGGGCA 59.5 0.08
mm4_intergenic_Gm21070|Jrkl_chr9_13198779_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm21070|Jrkl_chr9_13198779_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm10797|Zfp365_chr10_67770411_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm10797|Zfp365_chr10_67770411_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Pabpc2|Yipf5_chr18_39802872_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Pabpc2|Yipf5_chr18_39802872_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Shroom4_chrX_6405666_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Shroom4_chrX_6405666_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_D3Ertd751e|Gm25714_chr3_42129719_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_D3Ertd751e|Gm25714_chr3_42129719_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Lyzl6|Rprml_chr11_103644198_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Lyzl6|Rprml_chr11_103644198_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Rsrc1_chr3_67109645_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Rsrc1_chr3_67109645_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Park2_chr17_11267881_F TCGTCGGCAGCGTCATTGCAGCAGGAAGGAGCAT 60.0 0.07
mm4_intron_Park2_chr17_11267881_R GTCTCGTGGGCTCGGGAACTACAGACCACCCAGCC 60.0 0.07
mm4_intergenic_Acer3|Tsku_chr7_98321413_F TCGTCGGCAGCGTCGAGCAAAGTGGGACAGAGTGA 59.9 0.07
mm4_intergenic_Acer3|Tsku_chr7_98321413_R GTCTCGTGGGCTCGGCACCACAGCACTAACTCCCA 59.6 0.07
mm4_intron_Gbp2b_chr3_142595635_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Gbp2b_chr3_142595635_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm22508|Ccdc68_chr18_69788341_F TCGTCGGCAGCGTCTGAAAGCTGGAGGTGTTCAAGT 60.0 0.06
mm4_intergenic_Gm22508|Ccdc68_chr18_69788341_R GTCTCGTGGGCTCGGTCCTTGGCTTGACTACAGCTG 59.9 0.06
mm4_intergenic_Slfn1|Slfn4_chr11_83162892_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Slfn1|Slfn4_chr11_83162892_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Slfn1|Slfn4_chr11_83151222_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Slfn1|Slfn4_chr11_83151222_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Slfn1|Slfn4_chr11_83139535_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Slfn1|Slfn4_chr11_83139535_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Slfn1|Slfn4_chr11_83127851_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Slfn1|Slfn4_chr11_83127851_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Hace1_chr10_45655127_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Hace1_chr10_45655127_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm13211|Gm13254_chr2_7907356_F TCGTCGGCAGCGTCCATCAGGGAGCTCTCTGCAC 60.1 0.06
mm4_intergenic_Gm13211|Gm13254_chr2_7907356_R GTCTCGTGGGCTCGGACCAAGCAAGTACTTTACAGACT 57.7 0.06
mm4_intergenic_Gm12183|Gnb2l1/Gm25296/Snord96a/Snord95_chr11_48776518_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm12183|Gnb2l1/Gm25296/Snord96a/Snord95_chr11_48776518_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm17566|Gm23153_chr1_190469619_F TCGTCGGCAGCGTCTCCCTAGCAGTTGTGAAAGCT 59.3 0.06
mm4_intergenic_Gm17566|Gm23153_chr1_190469619_R GTCTCGTGGGCTCGGACGATTTCTGATGGTGGGGT 59.0 0.06
mm4_intron_Slc44a3_chr3_121523278_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Slc44a3_chr3_121523278_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_3110039M20Rik|Gm9804_chr12_49394183_F TCGTCGGCAGCGTCACTCGAAATTATGCCAAGGGTC 58.7 0.06
mm3_intergenic_3110039M20Rik|Gm9804_chr12_49394183_R GTCTCGTGGGCTCGGTGCACACTAATAAAACATATGTGCA 57.8 0.06
mm3_intergenic_Gm25384|Cxcr4_chr1_128515647_F Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Gm25384|Cxcr4_chr1_128515647_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Rbm34|Gm22730_chr8_126998393_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Rbm34|Gm22730_chr8_126998393_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Nek10|Lrrc3b_chr14_15308540_F TCGTCGGCAGCGTCTGCCCTCAGAACATTCACTGG 60.2 0.06
mm4_intergenic_Nek10|Lrrc3b_chr14_15308540_R GTCTCGTGGGCTCGGCAGGCTTCTCGTTCCCCAAA 60.2 0.06
mm4_intergenic_Rock1|Greb1l_chr18_10317053_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Rock1|Greb1l_chr18_10317053_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm25178|Eefsec_chr6_88408985_F TCGTCGGCAGCGTCATGTCAAAGGCTTGCTTGGC 59.6 0.05
mm4_intergenic_Gm25178|Eefsec_chr6_88408985_R GTCTCGTGGGCTCGGCACTCCTGCTCGTGGAAACT 59.9 0.05
mm4_intergenic_Gprin3|Gm22622_chr6_59685958_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gprin3|Gm22622_chr6_59685958_R Primer3: not found at this Tm N.d. 0.05
mm4_exon_Psd4_chr2_24394189_F TCGTCGGCAGCGTCACCACCTCAGACCCATGGAA 60.4 0.05
mm4_exon_Psd4_chr2_24394189_R GTCTCGTGGGCTCGGGTTCAGAAGGGCCTGGATGG 60.3 0.05
mm4_intergenic_4933434I20Rik|Clgn_chr8_83387024_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_4933434I20Rik|Clgn_chr8_83387024_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Polr3b_chr10_84652297_F TCGTCGGCAGCGTCTGTAGCTCTTGTCATAGCAGTCT 58.9 0.05
mm4_intron_Polr3b_chr10_84652297_R GTCTCGTGGGCTCGGAGCAGCCATCAACTCTTCACT 59.6 0.05
mm4_intergenic_Gm26235|Gm25392_chr18_19395431_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm26235|Gm25392_chr18_19395431_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Dpy19l2_chr9_24692999_F TCGTCGGCAGCGTCAGCTATTCATCTGGGCTAACAG 57.6 0.04
mm4_intron_Dpy19l2_chr9_24692999_R GTCTCGTGGGCTCGGGAGCCCAGCATATCCCAGTT 59.5 0.04
mm4_intergenic_Gm12384|Gm12386_chr4_39824581_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm12384|Gm12386_chr4_39824581_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Mageb18|Gm14777_chrX_92278452_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Mageb18|Gm14777_chrX_92278452_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Opcml_chr9_28743757_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Opcml_chr9_28743757_R Primer3: not found at this Tm N.d. 0.04
mm2_intergenic_Plcl1|Hsfy2_chr1_56483952_F TCGTCGGCAGCGTCTGGCCTCCAACACATTGACT 59.5 0.04
mm2_intergenic_Plcl1|Hsfy2_chr1_56483952_R GTCTCGTGGGCTCGGCTTGTCTGTCTGGGTGCAGA 59.6 0.04
mm2_intron_Rapgef4_chr2_72032316_F Primer3: not found at this Tm N.d. 0.03
mm2_intron_Rapgef4_chr2_72032316_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Csmd1_chr8_16469412_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Csmd1_chr8_16469412_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Crp|Gm24817_chr1_172780096_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Crp|Gm24817_chr1_172780096_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm25619|Cfhr2_chr1_139808852_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm25619|Cfhr2_chr1_139808852_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Siglec1|Hspa12b_chr2_131106107_F TCGTCGGCAGCGTCGGTCTCTCTTCAGCACTCGG 59.8 0.03
mm4_intergenic_Siglec1|Hspa12b_chr2_131106107_R GTCTCGTGGGCTCGGGTCTGAGTGAGTCTGCAGGG 59.7 0.03
mm4_intergenic_4930467D21Rik|Antxr2_chr5_97823010_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_4930467D21Rik|Antxr2_chr5_97823010_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Kdm5b_chr1_134580990_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Kdm5b_chr1_134580990_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm23708|G6pd2_chr5_61395920_F TCGTCGGCAGCGTCTCAGGAGAAAGCACCGATTGT 59.6 0.03
mm4_intergenic_Gm23708|G6pd2_chr5_61395920_R GTCTCGTGGGCTCGGTTCAAGCCCATCCTCATCCA 58.7 0.03
mm4_intergenic_Erbb4|Gm16076_chr1_68777500_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Erbb4|Gm16076_chr1_68777500_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Zfp619|Gm26526/Gm2058_chr7_39582328_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Zfp619|Gm26526/Gm2058_chr7_39582328_R Primer3: not found at this Tm N.d. 0.02
mm3_intron_Dmd_chrX_84776916_F TCGTCGGCAGCGTCAGTGCTTTAATGCTATGCAACTCT 59.0 0.02
mm3_intron_Dmd_chrX_84776916_R GTCTCGTGGGCTCGGGAGCATGGACACACAACACAC 60.0 0.02
mm4_intergenic_Apoo-ps|Zswim6_chr13_107503190_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Apoo-ps|Zswim6_chr13_107503190_R Primer3: not found at this Tm N.d. 0.02
mm3_intron_Cenpf_chr1_189669543_F Primer3: not found at this Tm N.d. 0.02
mm3_intron_Cenpf_chr1_189669543_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Fstl5_chr3_76594069_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Fstl5_chr3_76594069_R Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Mir3964|Gm23033_chr15_29727961_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Mir3964|Gm23033_chr15_29727961_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25292|Gm26667_chr16_84680619_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25292|Gm26667_chr16_84680619_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25258|Hao1_chr2_134456708_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25258|Hao1_chr2_134456708_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm20515|Sox2ot_chr3_34463244_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm20515|Sox2ot_chr3_34463244_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Helq_chr5_100785666_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Helq_chr5_100785666_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm6317|Gm25383_chr13_78851570_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm6317|Gm25383_chr13_78851570_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm6098|Zbbx_chr3_74483457_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm6098|Zbbx_chr3_74483457_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Megf11_chr9_64570113_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Megf11_chr9_64570113_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Xrra1_chr7_99886621_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Xrra1_chr7_99886621_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Zfp606|2900092C05Rik_chr7_12505163_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Zfp606|2900092C05Rik_chr7_12505163_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cacna1c_chr6_118601729_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cacna1c_chr6_118601729_R Primer3: not found at this Tm N.d. 0.01
mm2_intergenic_Cryge|Crygd_chr1_65053553_F Primer3: not found at this Tm N.d. 0.01
mm2_intergenic_Cryge|Crygd_chr1_65053553_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Arl8b|Edem1_chr6_108825761_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Arl8b|Edem1_chr6_108825761_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm23061|Ostf1_chr19_18454267_F TCGTCGGCAGCGTCCCATGTTTTCCTTCTGTTCTGTGT 59.6 0.01
mm4_intergenic_Gm23061|Ostf1_chr19_18454267_R GTCTCGTGGGCTCGGAAAAGTGTTGGCCTGCAAGG 59.5 0.01
mm4_intergenic_Gm22527|Rnf144a_chr12_25934937_F TCGTCGGCAGCGTCTTTCAAACTGGTGGCCCACA 60.3 0.01
mm4_intergenic_Gm22527|Rnf144a_chr12_25934937_R GTCTCGTGGGCTCGGCTGGAGTTGGCAGGTCTGAC 60.3 0.01
mm4_intergenic_Gm16194|Rab44_chr17_29134891_F TCGTCGGCAGCGTCAAGTCTCTGGGTGTGTTGCC 60.1 0.01
mm4_intergenic_Gm16194|Rab44_chr17_29134891_R GTCTCGTGGGCTCGGTTATGGTGGTGCTGGGAACG 60.3 0.01
mm4_intergenic_Gm15216|Mospd2_chrX_164907245_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm15216|Mospd2_chrX_164907245_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Ttll7|Gm23131_chr3_148188530_F TCGTCGGCAGCGTCTTCCCAAAGACTCACCCGTT 59.1 0.01
mm4_intergenic_Ttll7|Gm23131_chr3_148188530_R GTCTCGTGGGCTCGGTGACTCTGATGTTTATTTGCTCCA 58.4 0.01
mm4_intron_Gtf2h3_chr5_124591418_F TCGTCGGCAGCGTCGGTGATCATTGTCATTCTTGCACA 60.0 0.01
mm4_intron_Gtf2h3_chr5_124591418_R GTCTCGTGGGCTCGGCTGCCCAGGACCCTAAACAG 60.0 0.01
mm3_intergenic_Alcam|Gm25723_chr16_52596588_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Alcam|Gm25723_chr16_52596588_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Igf2r_chr17_12696714_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Igf2r_chr17_12696714_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Oxnad1_chr14_32100019_F TCGTCGGCAGCGTCCGAAGTGCCTGATGTAACACC 59.2 0.01
mm4_exon_Oxnad1_chr14_32100019_R GTCTCGTGGGCTCGGCAGAAGAAAACACGCTCCTGT 58.7 0.01
mm4_intron_Mylk_chr16_34955892_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Mylk_chr16_34955892_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm10858|Gtf3a_chr5_146898277_F TCGTCGGCAGCGTCTGAGAAGAGAACACACGGGC 59.9 0.01
mm4_intergenic_Gm10858|Gtf3a_chr5_146898277_R GTCTCGTGGGCTCGGTGTCTCATTGTGCTCCTGGC 60.3 0.01
mm4_intergenic_Ccdc50|Fgf12_chr16_28099135_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Ccdc50|Fgf12_chr16_28099135_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Smr3a|Smr2_chr5_88008594_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Smr3a|Smr2_chr5_88008594_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Cenpj_chr14_56529021_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Cenpj_chr14_56529021_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mdh1b_chr1_63701030_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mdh1b_chr1_63701030_R Primer3: not found at this Tm N.d. 0.00
mm3_intron_Tex36_chr7_133600590_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Tex36_chr7_133600590_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Spock1_chr13_57535948_F TCGTCGGCAGCGTCGCCAGCATGAGAACTTTGGG 59.4 0.00
mm4_intron_Spock1_chr13_57535948_R GTCTCGTGGGCTCGGTCAAAGGGGCTATCCTCAATTTCT 59.7 0.00
mm4_intron_Tenm4_chr7_96844137_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Tenm4_chr7_96844137_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Hdac9_chr12_34433457_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Hdac9_chr12_34433457_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25976|Acot10_chr15_20538752_F TCGTCGGCAGCGTCGTCTAACATCTCCTAAGTGTCACCT 59.5 0.00
mm4_intergenic_Gm25976|Acot10_chr15_20538752_R GTCTCGTGGGCTCGGTGTGTACTTTCCTAATGTGACTGA 57.5 0.00
mm4_intergenic_Gm25778|Ppapdc1a_chr7_129173063_F TCGTCGGCAGCGTCAGCCCCACATGAAAGAAGGG 59.9 0.00
mm4_intergenic_Gm25778|Ppapdc1a_chr7_129173063_R GTCTCGTGGGCTCGGTGAACTACTCTGAAAGGCAGTGA 59.3 0.00
mm4_intron_Slc8a2_chr7_16153085_F TCGTCGGCAGCGTCGCTTCAGCACTGTGGAATGC 60.1 0.00
mm4_intron_Slc8a2_chr7_16153085_R GTCTCGTGGGCTCGGAGAGAGGGTGAAGGGAGCAA 60.1 0.00
mm4_intergenic_Phlda1|4933440J02Rik_chr10_111569681_F TCGTCGGCAGCGTCTGGTACCCATCCCTTCCTGT 59.8 0.00
mm4_intergenic_Phlda1|4933440J02Rik_chr10_111569681_R GTCTCGTGGGCTCGGGCACAGGTCAATGTTCCTCC 58.8 0.00
mm4_intergenic_Vrk2|Gm12071_chr11_26666225_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Vrk2|Gm12071_chr11_26666225_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Smc5_chr19_23273261_F TCGTCGGCAGCGTCGAAAGGAGGATTGGACGGGG 60.1 0.00
mm4_intron_Smc5_chr19_23273261_R GTCTCGTGGGCTCGGACACGATTTCCAGAATGGGCA 60.2 0.00
mm4_intergenic_Gm25495|Gm23225_chr14_91004867_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25495|Gm23225_chr14_91004867_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_2310002F09Rik|Klk13_chr7_43711219_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_2310002F09Rik|Klk13_chr7_43711219_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Olfr27_chr9_39131596_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Olfr27_chr9_39131596_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Zfp207_chr11_80402756_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Zfp207_chr11_80402756_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_G930045G22Rik_chr6_50853986_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_G930045G22Rik_chr6_50853986_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Tacc2_chr7_130725743_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Tacc2_chr7_130725743_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rad18_chr6_112677907_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rad18_chr6_112677907_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mapk12_chr15_89138265_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mapk12_chr15_89138265_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Bbs9_chr9_22801061_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Bbs9_chr9_22801061_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Speer2|Gbe1_chr16_70287685_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Speer2|Gbe1_chr16_70287685_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pros1|Epha3_chr16_63120653_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pros1|Epha3_chr16_63120653_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25176|Smarca2_chr19_26353842_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25176|Smarca2_chr19_26353842_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Fer|Pja2_chr17_64188350_F TCGTCGGCAGCGTCGCTGCTCTGTGTTTGGCAAA 59.8 0.00
mm4_intergenic_Fer|Pja2_chr17_64188350_R GTCTCGTGGGCTCGGCCTGTGCTTGCTCATCTGGA 60.0 0.00
mm4_intergenic_Chst11|1700025N21Rik_chr10_83121498_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Chst11|1700025N21Rik_chr10_83121498_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_4930412D23Rik|Gm25061_chrX_127842858_F TCGTCGGCAGCGTCTTTGAGGCACAGACCTTGGG 60.1 0.00
mm4_intergenic_4930412D23Rik|Gm25061_chrX_127842858_R GTCTCGTGGGCTCGGGCATGGGGTTGGAGTTGTCA 60.5 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm4_intergenic_Igkv9-123|Igkv1-122_chr6_67998285 AACAAGGTTTCTTTCATTTGTGTTTATTAAAGCCTATATAGAAACATAATCATATGTATGTATATGACACAAATTAAAAG
TGAAACTTTCCAGAGTAACAAACTGTTAGGTTTTGGATGTGA
mm4_intergenic_Xkr4|Gm1992_chr1_3454982 CCCACATTGGTGATGTCCCATGGAAGATAGAGTGGCACCTCTCCCCTATCCAGAAATATCAACATGCTGTCACTCTACCA
CGTCTCCTTTCAGTGG
mm4_intergenic_mmu-mir-6342|Gm23771_chr1_29914213 TGTTCAGTCTGCAGATTGAGGAATTTAATTCATTAGAGCCTCATGTTGATGTTTCAGGATCGGTGTTGGGTACCTCAACT
GTTT
mm3_intergenic_Prdm4|Ascl4_chr10_85917791 GGCAATATCCCATACCCCACAATTCACTAATCATACCTAAGTATTTACCCAATACAGAAACANNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNCAAAGACATATGTACTAAGTTCCTTGCAACTTTGTTAATGATCTGT
mm4_intron_Cnga2_chrX_71993671 AGGATGGATGGGGAAGAGGAGAGAGATTTCAGCAGTAGAATAAGCAAGATTTAGGAGTTTTGCAGGAGGTATCAAAGATA
ATGGGTAAGTTTCTGGATTGG
TGGCTTGTGAGGAAAGATGAGC
mm4_intergenic_Gm833|Gm25779_chr4_152878411 ACACACACAAAGCTCCCTCATCCTTCTGAGACACAAGCAAGATGGAGGAACATAGTTATTCTTCAGGATTGGTCTTCCTT
GGCTCTTTGTTGCTGATCTGGACTCCAGAGACACTTCCG
mm2_intergenic_Gm13468|Gm13481_chr2_48004953 GCGGGACAAGAGGTTAAAATGGTTAGGCTGAATGCTATTGATTTATACAAGGATTTCCAATCCAGAAACATAAACAGGTT
CTGAGGAAAAGTCTCCTTTCTCTAGAAGACTTCTAATTCTAACTGATGCAATCCCTGTGA
mm4_intergenic_Gm23558|Galc_chr12_97974206 ACGAATGCAACGGTAGTTTGCGAATTTATTTTCCGCAGATGAAATCGATATGTTTCTGGAAAGGACTTTACCCTGCGTTT
ATTCACTGGGGAGCATTTGTGTCCT
mm4_intergenic_Lzts3|Ddrgk1_chr2_130651698 AGATTTGCTTGAGTTACATGAGAGCCTATCTAGAAACAGAAACAAGTGTTTAAAATATTTATATGTTAACTTTTTGTGTG
TAGTGCTAAAAATCAAACATGAGACTTTATATATGCTATATCCACAGCCCTGAG
mm4_intron_Tmem163_chr1_127596556 CATCCACCGGTTCAGATGCTTGGTAAGCAGATAAAGTAACATCATTTTGATCAGACATATAACGCGCCCAATTCAGAAAC
ATAGCCAATT
ACTTACTCCTCATGGATTCAAGAATATAAGCTAAGATGGAATGTGCTACA
mm4_intergenic_Gm6806|Gm22332_chrX_68545508 ACTTTAATCCCTTCTGAGAGGAGACCCCAACCAGTGACATAACCATATTCTATTAGATTCTATCAACACTTGACATCATC
AAGCTGAAGTTTAGGTTTCTAAGACATGAAATCTTGTAAGCAACCTGT
mm4_intergenic_Gm22483|Prelid2_chr18_41099431 TCCCATAGGAATTCAAGACAAGCTAAATGCTAAAAAATTTTCTGTTTATTGTGTAAGGTAAAGAATGATGTACCACAGCC
ATGTTTCTGTTTCTGAATGGG
TGGTAGTAGCCAAGAGAGGCAGTAGATACTCAGCA
mm4_intergenic_4833403I15Rik|Arl14epl_chr18_46916547 CTGATGGGCTGATTGATGAGGAAATTTACTATAAGTTGAGTACAGTTAACAGTCTTCAAATGTTACTTTTAAAATTTACT
ATGTGTACCATACATGCATATATTTCTGGGTGGGTGTATAAGTGTGTGGTGCTTGTAGGT
mm4_intron_Arhgap29_chr3_122001886 GCTCTTTCTCACGTGGCTCTTTCTCTCATATTTCAAATGTCAGCTCTTTGGCCTTCCCTAACCTATCATGTTAAAAATAC
CCTTCCAGAAACACAAGCATGG
TGATAAAGCAGGGGATAGGATAGCA
mm4_intron_Arhgap15_chr2_44384697 TCTCTGGTAACTCACTGATACTCTAAAAATATGAGACTCCCTTCCAGAAACGTAGCCATGCCAACAATACTCACAGGTAC
AAATGTTCTCCATTTAAAGCAGGAGGAAAAGAAAAATGTGAAGCGGGATGTCTTGGA
mm4_intergenic_Myc|Pvt1/PVT1_1_chr15_61991568 GACACACGCTCATCAATGGCCCATGAGAAAGCCTTTTGGAACAGGTATCTTATTAATTATAAGCCTCTGAAAAGCCACAA
TCCAAACCAGAAACTGAAACATGTAGGTAAAGTCCAGGGAGAAAGGTACC
mm4_intron_Slco1a6_chr6_142088626 ACATCCCCTGCCATAAACCCTTTGAACAGCACAAAGGCTCACTTTTCTCTTCAAGAAGTTTTCAGAATAGTGCCTATCCA
GAAAAAGAACCATAG
GTGCAAATTCCTTTAGGCTGAA
mm4_intergenic_Utp20|Slc5a8_chr10_88850381 TTTACCGTAAGACGCAGGCGGTTGTGCACATGTTTATGTATCTGTTTAGGAGAAGGGGATGGTTTAAGTGAGAATTTTCC
CCATCAGTCTTGGGAAGCAGTGTAACTCAAGCAAGACTGGCTGAGCTT
mm4_intergenic_Gm23131|Lphn2_chr3_148602649 CCCAACCCAAACACAAGCAGTTCTTGGAGAAGTGAAAGTCCCTACCCAGAAACATATCCTTGACATTAAGACCTAGTCAC
TGTGAGTTGC
mm3_intergenic_Gm4884|Gm2128_chr7_41097014 AGAGGTGACCCATTGACAACAATATAAGATTTTGTAGATGTTTTCTCTAATCCAGAATCATAACCAAGCCCAAATATTTC
ATTTCCTTATGTCAATTTGCATATCATTTACAGGAAGTCTGTGAGATACAGTGCTGGAAG
mm4_intergenic_Ralgps2|Tex35_chr1_157041581 TGGAGAGGCGGTGAATTCAGACATGGTAAAGTTTCTGGGAAGGAGCTGCAGCTACTGAGATAGAAGGAAACCCATCTTAG
GAGAATCATTGGAAGTCCATAACATCTCGTGTCTTCTCCTTCTCCTCCTTTCCCAAGCC
mm4_intron_Ksr2_chr5_117611521 GGCTCCCCACAAAGGACTTTGTCCCCTATCCAGAAACAAAAGCTTTTCCTGACAAAGGACAGAGGCAAGAGGAACC
mm4_intergenic_Gm4922|Perp_chr10_18842007 CCTCCCACAGGTTGTGTAGACAGAGTCCCACCATTAGTTAGATTTCTACTTCCTGAATACTCCAAAGGCCACAGGCAGCC
AGGACAGAATTATGTGTCTGGATGGGGCAGGGGAGCGGAAGTGGCTGTTGTGTCA
mm4_intron_Galnt18_chr7_111706832 CCTAATTAATAGGGTCAGTGTCCTAAGAAGGTTCTGTTTCTGTATTGGTGCCCAGTGATACACACAGAGCTTCCCTGACC
CACGTGCTCTGGCCTCTTTA
mm3_intergenic_Chrne|Gm12315_chr11_70620575 AGCCCTTTATGCGTTACACCTTATGCATCACCCCAATCCAGGAAAATAATCATGTCTGTCCTGAATTTGTGAAAAGATCT
GTAGCGGAGGGTCACT
mm3_intergenic_Fam173b|Tas2r119_chr15_32119260 GAGCCGCTGTCTGAGAAGTTGAAGGAATCTAATCCAGAAACACAACCAAGGCTGCTACCTTCTCTGTGGAGTGGAGAACA
GAATTCTGGCCTTCCTGAGAATAGAACTGTGGAGACTCTGAAGCATGGTGGAGC
mm4_intergenic_Fbxw11|Gm8192_chr11_32753910 GGTGCACACCCTATGACTTCAGAGGCCCAACTACTTACAGCTCTGTAGAATTGACTTTCCTAGCCAGAAACATGACCTTG
A
GGACAAGACCTTTATTTGTCTTGTTTAGAGGTGGAGCACAAGGC
mm3_intergenic_Adra2a|Gpam_chr19_54412906 CACTTGGCCCTCGATGAAGTTCTCATGGTTAACTTTCTGGATGAGCAAACTGAGACACAAGAGGTTTGAGGCACGCAGCC
CCCAAAGGTGGCTAAGCTAGGGTGA
mm4_intergenic_Gm26871|Dpyd_chr3_118541161 TCAGTGTTGGGATGGATGGTTATAGAAAAGCAACATGGTTAAGATAATGGATTGGAGTTGGAGGAAGCTTCAAAACAGAC
T
mm4_intergenic_Dok5|Gm14263_chr2_170912170 TCTCAAAGTCAAAATGAAAGTTGGATCTACTCCAACCTGCCAGTGTTCTGTTTCTGGATAGGCGGATAAGGGAATGAGTT
TGTCAGTTTGACACATTGGGTTCAGGGCTCT
mm4_intron_Col23a1_chr11_51527207 GGGATGCAAAACGGGAAGACTAAGGAAACACGGAGACTAAGGCATGGTTTTGTATCTGGCTCGGTTGTTAAAATATTAAA
TGGGTTAAGCAAGCAGGAATTCTTAAGCTACCACTGCAAGGGGCTTGAAGGGGTAG
mm4_exon_Hdac11_chr6_91174675 TCCAGGTTGTTGCTGTGTGTTCTGAGGTAGACAGACCTGCCTCCTGAGGACCACTGGAAATAAAGATTCAAAATTCACCA
ATCCAGACACTTAACCAGGG
CAGCTGCCCAAGATGAGTGGTTGT
mm4_intron_Park2_chr17_11267881 ATTGCAGCAGGAAGGAGCATGACGTCACCTCCCTCAAGGTTGTGTTTATGGATGGGAATTCTCACAGCTCATCTCGGTGG
CTGGGACGTCTTGGCTTGTTGGTGATGGTTCACGGCTGGGTGGTCTGTAGTTC
mm4_intergenic_Acer3|Tsku_chr7_98321413 GAGCAAAGTGGGACAGAGTGACAGAAATCCTGTTCCAAAGACAAAAAGAAGCCATCCTATCCAGAAAAATAAAAATATAC
AGATTCTCAACAGCAATAATGAACTGGGAGGCCTACTCCTTGGGAGTTAGTGCTGTGGTG
mm4_intergenic_Gm22508|Ccdc68_chr18_69788341 TGAAAGCTGGAGGTGTTCAAGTTAACGACAAATGCTTCAGCACTTTCCTGCCCATGCATAAACATCTCCATGTAAAATAG
AAAGGGGTGCTCTGTTTATCACAGCTGTAGTCAAGCCAAGGA
mm4_intergenic_Gm13211|Gm13254_chr2_7907356 CATCAGGGAGCTCTCTGCACCTTTTGCAATGATTAACATGGGTATTTCTCTGGGTTGGGTGGTTACCTTAGTCTGTAAAG
TACTTGCTTGGT
mm4_intergenic_Gm17566|Gm23153_chr1_190469619 TCCCTAGCAGTTGTGAAAGCTTGGATAAGGTTCGAAACTTCTTTAAATCTAGTACTTCCTATTCAGGAACCTAAACATGT
CATCATTGGGTTATTATGAGCACAACATGATATTCAACATACCCCACCATCAGAAATCGT
mm3_intergenic_3110039M20Rik|Gm9804_chr12_49394183 ACTCGAAATTATGCCAAGGGTCCTTATCCAGAAAAATAGCCATATAATGTCCTATATATTAAATACATGTATGCACATAT
GTTTTATTAGTGTGCA
mm4_intergenic_Nek10|Lrrc3b_chr14_15308540 TGCCCTCAGAACATTCACTGGGTTCTAGATTCTGGGCAGAAAATTCTGCATGGGTAAACAGTAAAGGATTTATGAACAGA
GAACTGTCCTGTCCAGAAGTATAACCAAGTGGGAGTTTGGGGAACGAGAAGCCTG
mm4_intergenic_Gm25178|Eefsec_chr6_88408985 ATGTCAAAGGCTTGCTTGGCCTGAAAAGTGAAGTCCTAGGCAGAAACATGCCCATGTCTTAGAGAAGAAAGAGTTCAGGC
TAACAAAAGTGCTTCCACAAGTTTCCACGAGCAGGAGTG
mm4_exon_Psd4_chr2_24394189 ACCACCTCAGACCCATGGAATGTCTCAATATTTGCTTGGAAGACAACTTACAACCCTGTCCAGAAGCATATCCAAGTGAA
ATACATGGCCATCCAGGCCCTTCTGAAC
mm4_intron_Polr3b_chr10_84652297 TGTAGCTCTTGTCATAGCAGTCTTCACATGGTTTGGTTTCTTTATGGGGAAACTCTGCATTCCACAGTGACATTCTGAGT
GAAGAGTTGATGGCTGCT
mm4_intron_Dpy19l2_chr9_24692999 AGCTATTCATCTGGGCTAACAGAATAAATGCAAAACAACCCAATGCAGAAACATAAAATTGTCATGTTGAAATCCCTAAC
ACAACTCCTCTTTGCCTATATAACTGGGATATGCTGGGCTC
mm2_intergenic_Plcl1|Hsfy2_chr1_56483952 TGGCCTCCAACACATTGACTCACAATGCCACAGACTGTGAATAGCTTTCCATGGCTATGTTTCTGGATAGAACCATAAAA
TATTTTCATAGATGTCATAAGGGGAAGATTTTCTATCTGCACCCAGACAGACAAG
mm4_intergenic_Siglec1|Hspa12b_chr2_131106107 GGTCTCTCTTCAGCACTCGGTGCTCCCCTTTTCCCTATTCTCTAGCCCCAACCAGGAAGCTAACCATGTGCTTTCTTCCT
TTAGCTACACAGATGTCATTGATGTTGTGCAAGCCCTGCAGACTCACTCAGAC
mm4_intergenic_Gm23708|G6pd2_chr5_61395920 TCAGGAGAAAGCACCGATTGTAGGACTTTTCTCTGTGTAGAAAGTAGAACCTCTTAAAAGAACAATAGGGTGTGGATAGA
CAGGGTGATGTTTCTGGGGAGG
TGGATGAGGATGGGCTTGAA
mm3_intron_Dmd_chrX_84776916 AGTGCTTTAATGCTATGCAACTCTAAAATTCTGAGATAATGCATTTTCAATCCAGAAACATCACTGTGTGTGTGTTGTGT
GTCCATGCTC
mm4_intergenic_Gm23061|Ostf1_chr19_18454267 CCATGTTTTCCTTCTGTTCTGTGTTCTGTATCACAATGAAATGGTTTTGTTTTTGGTTTGGTTNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNTTTTAAATAAGTCCCTTGCAGGCCAACACTTTT
mm4_intergenic_Gm22527|Rnf144a_chr12_25934937 TTTCAAACTGGTGGCCCACAGCCCCCTCCAGACCCATAAACATGTTTATGTGCCTCACTCCATCATTCTTCATCCCAGGA
AACATTTATATCCGAAGAATTTCACATAAAAAGTCAGACCTGCCAACTCCAG
mm4_intergenic_Gm16194|Rab44_chr17_29134891 AAGTCTCTGGGTGTGTTGCCATTAAAACTGAGGACTACGGTTAGGGATATGGTTTTGTTTGTGGAATGGCAACATACATG
AATCTGTCTGTGGGACCCGTTCCCAGCACCACCATAA
mm4_intergenic_Ttll7|Gm23131_chr3_148188530 TTCCCAAAGACTCACCCGTTAACATTTAATTACTGTGTCCTTTTGATATTTTAAATATCATCTGAAGATGGTTTTGTATG
TGGATAGG
TAAGAGTTTAAACCAAAATGGAGCAAATAAACATCAGAGTCA
mm4_intron_Gtf2h3_chr5_124591418 GGTGATCATTGTCATTCTTGCACAGTAGTGTTCTGGCTAAACAGGGCTGTGTTTGTGGATCGGGGATTCAGAAGCCACAC
TGGGTGCTTGTCATTTTTCTGTTTAGGGTCCTGGGCAG
mm4_exon_Oxnad1_chr14_32100019 CGAAGTGCCTGATGTAACACCAGAATGTATTATCTGATCACCCAGTGAAAATGTCTTCTATGAAGCCCATCACTAAACAG
TGAATATATGCCAATCCAAATAAATAAACATGTAAATAAACAGGAGCGTGTTTTCTTCTG
mm4_intergenic_Gm10858|Gtf3a_chr5_146898277 TGAGAAGAGAACACACGGGCTGGGCCAGGGCAGGGATGGCCTTCTGAAAAACTCTGGGTCGCCGCACCTGGTTGTGTTNN
NNNNNNNN
NNNNNNNNNNNNNTGTCGGCCAGGAGCACAATGAGACA
mm4_intron_Spock1_chr13_57535948 GCCAGCATGAGAACTTTGGGGGATACCTTCAGATCATATAGTGATATGGTTATGATTTTGGAGGGGAGGGAATACCAGGT
ATTTTTAGTGATAATGATCAGTATAGAAATTGAGGATAGCCCCTTTGA
mm4_intergenic_Gm25976|Acot10_chr15_20538752 GTCTAACATCTCCTAAGTGTCACCTACCCCCTCCTAAAACAAAACCATGTTTTATTTATTCCTTGCATGTATTATTTATT
CCAATGGTTTTATAACAATTACATCATCAGTCACATTAGGAAAGTACACA
mm4_intergenic_Gm25778|Ppapdc1a_chr7_129173063 AGCCCCACATGAAAGAAGGGCAAAGACTTGAACCATTATCAAATGCCCAATGCCTAGACAAAAACATAAGCATGTTCACT
GCCTTTCAGAGTAGTTCA
mm4_intron_Slc8a2_chr7_16153085 GCTTCAGCACTGTGGAATGCACATGGTTACCTTCCTGGACAGGGCATGTAGAGACAGCCCCACAGCCCCCAGCCACACCA
CCCTGGGATGCTGTAAAACTAATTGCTCCCTTCACCCTCTCT
mm4_intergenic_Phlda1|4933440J02Rik_chr10_111569681 TGGTACCCATCCCTTCCTGTACTCTGCATTTACCCATCCACAAAGGTAACCTTGTCTTTGTTTGAAAGCTCTCTCCCTAG
CTATCCTGATTCCATTAGCAGCTTGTTTCTAAGGAGGAACATTGACCTGTGC
mm4_intron_Smc5_chr19_23273261 GAAAGGAGGATTGGACGGGGCAAGAATCTGCAAGGAGGTACAAAAACTGGGAACACGATTTTTAATTTCTTTGGTACACT
CTAATCTCCAATCAACAACCATTACCATGTCATCTGCCCATTCTGGAAATCGTGT
mm4_intergenic_Fer|Pja2_chr17_64188350 GCTGCTCTGTGTTTGGCAAACCTTGTGGCTTGGCTTTTGTGCCTAGATTGGTTTCCCAACCAACAACATAACCAGGTGCT
CAAGGCCTGAGAGTGGCACACGCCCTGTCTCATTTTCTCCAGATGAGCAAGCACAGG
mm4_intergenic_4930412D23Rik|Gm25061_chrX_127842858 TTTGAGGCACAGACCTTGGGCATGGTGTCATGGGATACCACTGAGATCCCATCTAGCAACACTACCATGTGAGTGACAAC
TCCAACCCCATGC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.