CRISPOR

DNA break repair outcome predictions

Guide: TTGGCTGAGAAGAGGAGCTA AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ATCCCAGGCTCCCTGGGATCTTGGCTGAGAAGAGGAGCTAAGGCGACAAGGCAGGCGCCGGGTAGCCCCAGCGCCGTGTC`	(Wild-type sequence)
335	`CCCTGGGATCTTGGCTGAGAAGAGG--------CGACAAGGCAGGCGCCGGGTAGCCCCA`	`8 bp deleted → frameshift`
296	`CCCTGGGATCTTGGCTGAGAAGAGGAGC------GACAAGGCAGGCGCCGGGTAGCCCCA`	`6 bp deleted → no frameshift`
233	`CCCTGGGATCTTGGCTGAGAAGAGGAG-----GCGACAAGGCAGGCGCCGGGTAGCCCCA`	`5 bp deleted → frameshift`
232	`CCCTGGGATCTTGGC-------------------GACAAGGCAGGCGCCGGGTAGCCCCA`	`19 bp deleted → frameshift`
224	`CCCTGGGATCTTGGCTGAGAAGAGG----------------CAGGCGCCGGGTAGCCCCA`	`16 bp deleted → frameshift`
198	`CCCTGGGATCTTGGCTGAGAAGAGGAGC--------------AGGCGCCGGGTAGCCCCA`	`14 bp deleted → frameshift`
184	`CCCTGGGATCTTGGCTGAGAAGAGG--------------------CGCCGGGTAGCCCCA`	`20 bp deleted → frameshift`
182	`CCCTGGGATCTTGGCTGAGAAGAGGA----------CAAGGCAGGCGCCGGGTAGCCCCA`	`10 bp deleted → frameshift`
182	`CCCTGGGATCTTGGCTGAGAAG----------GCGACAAGGCAGGCGCCGGGTAGCCCCA`	`10 bp deleted → frameshift`
162	`CCCTGGGATCTTGGCTGAGAAGAGGAGC------------------GCCGGGTAGCCCCA`	`18 bp deleted → no frameshift`
162	`CCCTGGGATCTTGGCTGAGAAG------------------GCAGGCGCCGGGTAGCCCCA`	`18 bp deleted → no frameshift`
156	`CCCTGGGATCTTGGCTGAGAAGAGGAG-------------GCAGGCGCCGGGTAGCCCCA`	`13 bp deleted → frameshift`
156	`CCCTGGGATCTTGGCTGAGAAGA-------------CAAGGCAGGCGCCGGGTAGCCCCA`	`13 bp deleted → frameshift`
156	`CCCTGGGATCTTGGCTGAG-------------GCGACAAGGCAGGCGCCGGGTAGCCCCA`	`13 bp deleted → frameshift`
155	`CCCTGGGATCTTGGC---------------------------AGGCGCCGGGTAGCCCCA`	`27 bp deleted → no frameshift`
147	`CCCTGGGATCTTGGCTGAGAAGAGGAGC--------------------CGGGTAGCCCCA`	`20 bp deleted → frameshift`
134	`CCCTGGGATCTTGGCTGAGA----------------CAAGGCAGGCGCCGGGTAGCCCCA`	`16 bp deleted → frameshift`
128	`CCCTGGGATCTTGGCTGAGAAGAGGAG-----------------GCGCCGGGTAGCCCCA`	`17 bp deleted → frameshift`
127	`CCCTGGGATCTTGGC-------------------------------GCCGGGTAGCCCCA`	`31 bp deleted → frameshift`
123	`CCCTGGGATCTTGGCTGAGAAGAGGAGC----------------------------CCCA`	`28 bp deleted → frameshift`
122	`CCCTGGGATCTTGGCTGA------------------CAAGGCAGGCGCCGGGTAGCCCCA`	`18 bp deleted → no frameshift`
109	`CCCTGGGATCTTGGCTGAGAAGAGG--------------------------GTAGCCCCA`	`26 bp deleted → frameshift`
109	`CCCTGGG--------------------------CGACAAGGCAGGCGCCGGGTAGCCCCA`	`26 bp deleted → frameshift`
105	`CCCTGGGATCTTGGCTGAG---------------------GCAGGCGCCGGGTAGCCCCA`	`21 bp deleted → no frameshift`
103	`CCCTGGGATCTTGGCTGAGAAGAGG---------------------------TAGCCCCA`	`27 bp deleted → no frameshift`
103	`CCCTGG---------------------------CGACAAGGCAGGCGCCGGGTAGCCCCA`	`27 bp deleted → no frameshift`
99	`CCCTGGGATCTTGGCTGAGAAG----------------------GCGCCGGGTAGCCCCA`	`22 bp deleted → frameshift`
86	`CCCTGGGATCTTGGCTGAG-------------------------GCGCCGGGTAGCCCCA`	`25 bp deleted → frameshift`
76	`CCCTGGGATCTTGGC---------------------------------CGGGTAGCCCCA`	`33 bp deleted → no frameshift`
74	`CCCTGGGA----------------------------CAAGGCAGGCGCCGGGTAGCCCCA`	`28 bp deleted → frameshift`
73	`CCCTGGG----------------------------------CAGGCGCCGGGTAGCCCCA`	`34 bp deleted → frameshift`
69	`CCCTGG-----------------------------------CAGGCGCCGGGTAGCCCCA`	`35 bp deleted → frameshift`
63	`CCCTGGGATCTTGGCTGAGAAGAG-------------------------------CCCCA`	`31 bp deleted → frameshift`
63	`CCCTGGG---------------------------------------------TAGCCCCA`	`45 bp deleted → no frameshift`
62	`CCCTGGGATCTTGG-------------------------------------GTAGCCCCA`	`37 bp deleted → frameshift`
60	`CCCTGGGATCTTGGCTGAGAAGAGGAGCTA------------------------GCCCCA`	`24 bp deleted → no frameshift`
60	`CCCTGGGATCTTGG--------------------------------------TAGCCCCA`	`38 bp deleted → frameshift`
60	`CCCTGGG--------------------------------------CGCCGGGTAGCCCCA`	`38 bp deleted → frameshift`
57	`CCCTGGGATCTTGGCTGAGAAG---------------------------------CCCCA`	`33 bp deleted → no frameshift`
56	`CCCTGG---------------------------------------CGCCGGGTAGCCCCA`	`39 bp deleted → no frameshift`
51	`CCCTGGGATCTTGGC-----------------------------------------CCCA`	`41 bp deleted → frameshift`
49	`CCCTGGGATCTTGGCTGAG------------------------------------CCCCA`	`36 bp deleted → no frameshift`
44	`CCCTGGG--------------------------------------------GTAGCCCCA`	`44 bp deleted → frameshift`
40	`CCCTGG----------------------------------------------TAGCCCCA`	`46 bp deleted → frameshift`
40	`CCC----------------------------------------------GGGTAGCCCCA`	`46 bp deleted → frameshift`
38	`CCC-------------------------------------------------------CA`	`55 bp deleted → frameshift`
38	`CC-----------------------------------------------GGGTAGCCCCA`	`47 bp deleted → frameshift`
36	`CCC--------------------------------------------------------A`	`56 bp deleted → frameshift`
26	`CCC------------------------------------------------------CCA`	`54 bp deleted → no frameshift`
23	`CC---------------------------------------------------------A`	`57 bp deleted → no frameshift`

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