CRISPOR

DNA break repair outcome predictions

Guide: CCCCCCCTCTGGAGAGTCCG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAGGACATGGTTATGTTTCTGGATG`	(Wild-type sequence)
519	`TTTTCCCCCTCCCCCCCTCTGGAGAG---------------CCGAAAGGACATGGTTATG`	`15 bp deleted → no frameshift`
298	`TTTTCCCCCTCCCCCCCTCTGGAGAGTCCG--------------AAAGGACATGGTTATG`	`14 bp deleted → frameshift`
288	`TTTTCCCCCTCCCCCCCTCTGGA-----------TGGAGAGCCGAAAGGACATGGTTATG`	`11 bp deleted → frameshift`
261	`TTTTCCCCCTCCCCCCCTCTGGAGAG-------------AGCCGAAAGGACATGGTTATG`	`13 bp deleted → frameshift`
242	`TTTTCCCCCTCCCCCCCTCTGG----------GATGGAGAGCCGAAAGGACATGGTTATG`	`10 bp deleted → frameshift`
213	`TTTTCCCCCTCCCCCCCTCTGGAG-----------------CCGAAAGGACATGGTTATG`	`17 bp deleted → frameshift`
191	`TTTTCCCCCTCCCCCCCTCTGGAGA---------TGGAGAGCCGAAAGGACATGGTTATG`	`9 bp deleted → no frameshift`
129	`TTTTCCCCCTCCCCCCCTCTGGA---------------------------CATGGTTATG`	`27 bp deleted → no frameshift`
110	`TTTTCCCCCTCCCCCCCTCTGGAGA--------------------AAGGACATGGTTATG`	`20 bp deleted → frameshift`
109	`TTTTCCCCCTCCCCCCC--------------------------GAAAGGACATGGTTATG`	`26 bp deleted → frameshift`
103	`TTTTCCCCCTCCCCCC---------------------------GAAAGGACATGGTTATG`	`27 bp deleted → no frameshift`
99	`TTTTCCCCCTCCCCCCCTCTGGAGAG----------------------GACATGGTTATG`	`22 bp deleted → frameshift`
99	`TTTTCCCCCTCCCCCCCTCTGGA----------------------AAGGACATGGTTATG`	`22 bp deleted → frameshift`
98	`TTTTCCCCCTCCCCC----------------------------GAAAGGACATGGTTATG`	`28 bp deleted → frameshift`
96	`TTTTCCCCCTCCCCCCCTCTGG---------------------------------TTATG`	`33 bp deleted → no frameshift`
94	`TTTTCCCCCTCCCC-----------------------------GAAAGGACATGGTTATG`	`29 bp deleted → frameshift`
90	`TTTTCCCCCTCCCCCCCTCTGGAG------------------------GACATGGTTATG`	`24 bp deleted → no frameshift`
89	`TTTTCCCCCTCCC------------------------------GAAAGGACATGGTTATG`	`30 bp deleted → no frameshift`
86	`TTTTCCCCCTCCCCCCCTCTGGAGA-------------------------CATGGTTATG`	`25 bp deleted → frameshift`
84	`TTTTCCCCCTCC-------------------------------GAAAGGACATGGTTATG`	`31 bp deleted → frameshift`
73	`TTTTCCCCC----------------------------------GAAAGGACATGGTTATG`	`34 bp deleted → frameshift`
70	`TTTTCCCCCTCCCCCCCTCTGGAGAGT-----------------------------TATG`	`29 bp deleted → frameshift`
69	`TTTTCCCC-----------------------------------GAAAGGACATGGTTATG`	`35 bp deleted → frameshift`
66	`TTTTCCC------------------------------------GAAAGGACATGGTTATG`	`36 bp deleted → no frameshift`
62	`TTTTCC-------------------------------------GAAAGGACATGGTTATG`	`37 bp deleted → frameshift`
42	`TTTTCCCCCTCCCCCCCTCTG---------------------------------------`	`39 bp deleted → no frameshift`
14	`TTTT-----------------------------------------------------ATG`	`53 bp deleted → frameshift`
13	`TTT------------------------------------------------------ATG`	`54 bp deleted → no frameshift`
12	`TT-------------------------------------------------------ATG`	`55 bp deleted → frameshift`

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