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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313685_F | TCGTCGGCAGCGTCCGCATCACAAGGTGACCCTA | 59.7 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313685_R | GTCTCGTGGGCTCGGCTCCTAATCCCAGGCTCCCT | 60.1 | 1.00 |
| mm4_intergenic_D630032N06Rik|Gm22587_chr11_85297035_F | TCGTCGGCAGCGTCCAGCTCTTAGGCCTCATGGG | 59.8 | 0.84 |
| mm4_intergenic_D630032N06Rik|Gm22587_chr11_85297035_R | GTCTCGTGGGCTCGGGCTCAGACAGTGTTGGGTCA | 59.8 | 0.84 |
| mm4_intergenic_Mir5112|4930445N18Rik_chr18_82839191_F | TCGTCGGCAGCGTCGCATTGTTTGGGCCACCTTC | 60.3 | 0.38 |
| mm4_intergenic_Mir5112|4930445N18Rik_chr18_82839191_R | GTCTCGTGGGCTCGGGCCCATTAAGACAGAGCTCCA | 59.7 | 0.38 |
| mm4_intergenic_Rps16-ps3|3930402G23Rik_chr8_10884319_F | TCGTCGGCAGCGTCTTGCAGGGTGGTCTCATGAC | 59.9 | 0.35 |
| mm4_intergenic_Rps16-ps3|3930402G23Rik_chr8_10884319_R | GTCTCGTGGGCTCGGGACCCTGCAGACAACCATGA | 59.9 | 0.35 |
| mm4_exon_Angpt2_chr8_18699099_F | TCGTCGGCAGCGTCCTTTCCACGTCCTCTGGAAGT | 59.6 | 0.33 |
| mm4_exon_Angpt2_chr8_18699099_R | GTCTCGTGGGCTCGGACCAATGTTCAGCCTGCAGT | 60.1 | 0.33 |
| mm4_intergenic_Fgfr2|Gm5903_chr7_130296761_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Fgfr2|Gm5903_chr7_130296761_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Moxd1|Gm15271_chr10_24411098_F | TCGTCGGCAGCGTCTCCATGCAGCAGATGTAGCA | 59.4 | 0.30 |
| mm4_intergenic_Moxd1|Gm15271_chr10_24411098_R | GTCTCGTGGGCTCGGATTCCAGGCCTTAAGCGAGC | 60.4 | 0.30 |
| mm4_intergenic_Gm26256|n-R5s61_chr12_68348109_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm26256|n-R5s61_chr12_68348109_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_exon_mmu-mir-7667_chr17_29595580_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_exon_mmu-mir-7667_chr17_29595580_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Acaca_chr11_84358956_F | TCGTCGGCAGCGTCCGGTTTTCCCCTTCCCTCTC | 60.0 | 0.26 |
| mm4_intron_Acaca_chr11_84358956_R | GTCTCGTGGGCTCGGTAAAGGATGGGGAGGGCAGA | 59.9 | 0.26 |
| mm4_intron_Sfswap_chr5_129562572_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Sfswap_chr5_129562572_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_2610307P16Rik|Gm17528_chr13_28801183_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_2610307P16Rik|Gm17528_chr13_28801183_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm14089|Gm14088_chr2_119007165_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm14089|Gm14088_chr2_119007165_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_PISRT1|Prr23a_chr9_98783191_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_PISRT1|Prr23a_chr9_98783191_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_exon_4930513N10Rik/Cnot1_chr8_95806985_F | TCGTCGGCAGCGTCCGGGAGGAAAGGGACAGTTG | 60.3 | 0.18 |
| mm4_exon_4930513N10Rik/Cnot1_chr8_95806985_R | GTCTCGTGGGCTCGGCTGGAGGCTTCGAGGATGG | 59.5 | 0.18 |
| mm4_exon_Igsf10_chr3_59341268_F | TCGTCGGCAGCGTCGTACAGTGCACCTCTGTGGG | 60.3 | 0.17 |
| mm4_exon_Igsf10_chr3_59341268_R | GTCTCGTGGGCTCGGGCAGAAGAGAGGCAGGGAAG | 60.1 | 0.17 |
| mm3_intron_Golt1b_chr6_142395347_F | TCGTCGGCAGCGTCGAGACTGAACGGCTGTTTGC | 59.7 | 0.14 |
| mm3_intron_Golt1b_chr6_142395347_R | GTCTCGTGGGCTCGGCGCACAAATGACAGCACCTC | 60.1 | 0.14 |
| mm4_intergenic_Fitm2/2310001K24Rik|R3hdml_chr2_163487339_F | TCGTCGGCAGCGTCTGTGCATATGAAGTCATCAAAGGA | 58.5 | 0.12 |
| mm4_intergenic_Fitm2/2310001K24Rik|R3hdml_chr2_163487339_R | GTCTCGTGGGCTCGGTGTGTGAAGGTCTCTGGGAAG | 59.3 | 0.12 |
| mm4_exon_Hspa12b_chr2_131142668_F | TCGTCGGCAGCGTCCAGGAGACCGCTACATGGTG | 60.1 | 0.12 |
| mm4_exon_Hspa12b_chr2_131142668_R | GTCTCGTGGGCTCGGTAGAGCTCCTTGAGGGTCCC | 60.0 | 0.12 |
| mm4_exon_Gm6912_chr16_47076030_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Gm6912_chr16_47076030_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Bcat1_chr6_145017354_F | TCGTCGGCAGCGTCTAACAGCTTCACGCATGCAC | 59.4 | 0.11 |
| mm4_intron_Bcat1_chr6_145017354_R | GTCTCGTGGGCTCGGTCTTGCCATCTCTGACTGCA | 59.0 | 0.11 |
| mm4_intron_Rere_chr4_150430612_F | TCGTCGGCAGCGTCCCCTCCAGCTGATGTGAATGT | 60.0 | 0.11 |
| mm4_intron_Rere_chr4_150430612_R | GTCTCGTGGGCTCGGAACCAAGGCAGCCAATCAAA | 58.5 | 0.11 |
| mm4_exon_4430402I18Rik/Ppapdc2_chr19_28963903_F | TCGTCGGCAGCGTCGTGTCACAATCGTGCTCTGC | 59.8 | 0.09 |
| mm4_exon_4430402I18Rik/Ppapdc2_chr19_28963903_R | GTCTCGTGGGCTCGGCAGACCGGAGACAGGCTTC | 59.7 | 0.09 |
| mm4_intron_Dnajc19_chr3_34081014_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Dnajc19_chr3_34081014_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Slc24a2_chr4_87191269_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Slc24a2_chr4_87191269_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Rad51b|Zfp36l1/Gm26669_chr12_80008215_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Rad51b|Zfp36l1/Gm26669_chr12_80008215_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Rgs7_chr1_175464036_F | TCGTCGGCAGCGTCCCAGCTGCCCCTGAAGTTAT | 59.7 | 0.08 |
| mm4_intron_Rgs7_chr1_175464036_R | GTCTCGTGGGCTCGGAGGTTTTGTAAGCTCCACGC | 58.7 | 0.08 |
| mm4_intron_Pcsk2_chr2_143620642_F | TCGTCGGCAGCGTCAATAGCAGAAAGGACCACTAGG | 57.2 | 0.08 |
| mm4_intron_Pcsk2_chr2_143620642_R | GTCTCGTGGGCTCGGTGCCACAGCCTTTCTGCTA | 59.2 | 0.08 |
| mm4_intergenic_Gm13002|Wnt4_chr4_137251517_F | TCGTCGGCAGCGTCAAGACAAGGGTGCTGACTGG | 59.8 | 0.07 |
| mm4_intergenic_Gm13002|Wnt4_chr4_137251517_R | GTCTCGTGGGCTCGGAGTGTAGGGGTCACTTTGCC | 59.5 | 0.07 |
| mm4_intergenic_Park2|Agpat4_chr17_11871702_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Park2|Agpat4_chr17_11871702_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Ndufa12_chr10_94209893_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Ndufa12_chr10_94209893_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm15387|Bai1_chr15_74407439_F | TCGTCGGCAGCGTCAAGAAGCACTCTGTCCAGGG | 59.3 | 0.07 |
| mm4_intergenic_Gm15387|Bai1_chr15_74407439_R | GTCTCGTGGGCTCGGTAGCGGCTACCATTGGTGTG | 60.1 | 0.07 |
| mm3_intergenic_Gm24117|Gm25391_chr4_62888606_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Gm24117|Gm25391_chr4_62888606_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Tcf7l2_chr19_55806328_F | TCGTCGGCAGCGTCCGAGGCCAGGTTCTATGGTC | 59.8 | 0.06 |
| mm4_intron_Tcf7l2_chr19_55806328_R | GTCTCGTGGGCTCGGAGCCCAACAGCATCAAGATCA | 59.9 | 0.06 |
| mm4_exon_Gm10322_chr10_59616362_F | TCGTCGGCAGCGTCGGAGAGGTTCAAGTCCCGTG | 60.0 | 0.06 |
| mm4_exon_Gm10322_chr10_59616362_R | GTCTCGTGGGCTCGGGTGTCCTTCAGCGCCTGC | 61.4 | 0.06 |
| mm4_intergenic_Cacna2d3|Selk_chr14_29848054_F | TCGTCGGCAGCGTCTGGGCTTCATCAGTTGCTTCT | 59.9 | 0.06 |
| mm4_intergenic_Cacna2d3|Selk_chr14_29848054_R | GTCTCGTGGGCTCGGCCGAACAACTCTTGGGCTCT | 59.9 | 0.06 |
| mm4_intergenic_Anapc13|Amotl2_chr9_102640033_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Anapc13|Amotl2_chr9_102640033_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Pgpep1_chr8_70656615_F | TCGTCGGCAGCGTCCTGAGACACACTGGGGCTTT | 59.8 | 0.05 |
| mm4_intron_Pgpep1_chr8_70656615_R | GTCTCGTGGGCTCGGCCTTCACTCTGTGAGCCAGG | 60.0 | 0.05 |
| mm4_exon_H2-DMa_chr17_34138044_F | TCGTCGGCAGCGTCCTCTTTCCACCGACCCTGAC | 60.0 | 0.04 |
| mm4_exon_H2-DMa_chr17_34138044_R | GTCTCGTGGGCTCGGAGAAAGCCTGGAAGGTCAGC | 59.9 | 0.04 |
| mm3_intergenic_Anks1/Gm15598|Anks1_chr17_28032468_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Anks1/Gm15598|Anks1_chr17_28032468_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Snx3_chr10_42520579_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Snx3_chr10_42520579_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Gm648_chrX_56546526_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Gm648_chrX_56546526_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Ust_chr10_8206082_F | TCGTCGGCAGCGTCTCTTCAATGGCAGGCTCTGG | 60.0 | 0.03 |
| mm4_exon_Ust_chr10_8206082_R | GTCTCGTGGGCTCGGAGAATGCAACTAGAGAGGCC | 57.0 | 0.03 |
| mm4_intron_Zmpste24_chr4_121062461_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Zmpste24_chr4_121062461_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gpm6a|Adam29_chr8_55376527_F | TCGTCGGCAGCGTCAGCACCTCCCCAGAAGAGAA | 60.1 | 0.03 |
| mm4_intergenic_Gpm6a|Adam29_chr8_55376527_R | GTCTCGTGGGCTCGGGCTGCAAAGCCCAAGGAAAA | 59.8 | 0.03 |
| mm4_intergenic_Gpm6a|Adam29_chr8_55366648_F | TCGTCGGCAGCGTCTTGGAGAAGGGCCTCACAAC | 59.8 | 0.03 |
| mm4_intergenic_Gpm6a|Adam29_chr8_55366648_R | GTCTCGTGGGCTCGGGCTGCAAAGCCCAAGGAAAA | 59.8 | 0.03 |
| mm4_intergenic_Gpm6a|Adam29_chr8_55360015_F | TCGTCGGCAGCGTCTTGGAGAAGGGCCTCACAAC | 59.8 | 0.03 |
| mm4_intergenic_Gpm6a|Adam29_chr8_55360015_R | GTCTCGTGGGCTCGGGCTGCAAAGCCCAAGGAAAA | 59.8 | 0.03 |
| mm4_intergenic_Gpm6a|Adam29_chr8_55113182_F | TCGTCGGCAGCGTCAGCACCTCCCCAGAAGAGAA | 60.1 | 0.03 |
| mm4_intergenic_Gpm6a|Adam29_chr8_55113182_R | GTCTCGTGGGCTCGGTTTGGTGCGATTTTCGGCTC | 59.7 | 0.03 |
| mm4_intron_Mdga1_chr17_29888671_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Mdga1_chr17_29888671_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Trip10_chr17_57260816_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Trip10_chr17_57260816_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Shcbp1|Gm9457_chr8_4800986_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Shcbp1|Gm9457_chr8_4800986_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_4933400F03Rik/Kcnmb4_chr10_116473828_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_4933400F03Rik/Kcnmb4_chr10_116473828_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slc22a30|Gm6425_chr19_8407850_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slc22a30|Gm6425_chr19_8407850_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slc22a28|Slc22a29_chr19_8134710_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slc22a28|Slc22a29_chr19_8134710_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Ano1_chr7_144622615_F | TCGTCGGCAGCGTCTCTAGGGGATGCAGGTGACC | 60.6 | 0.02 |
| mm4_intron_Ano1_chr7_144622615_R | GTCTCGTGGGCTCGGTGTTCTGACACTGGGTTTGT | 57.2 | 0.02 |
| mm4_intergenic_Gm13652|A330043C09Rik_chr2_75143949_F | TCGTCGGCAGCGTCATGGCTACAAGGTGACCGAC | 59.7 | 0.01 |
| mm4_intergenic_Gm13652|A330043C09Rik_chr2_75143949_R | GTCTCGTGGGCTCGGCGAGGGAAGTACAGCAAGCA | 60.0 | 0.01 |
| mm4_exon_Gigyf1_chr5_137522669_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Gigyf1_chr5_137522669_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Gm20421/Hes5_chr4_154961383_F | TCGTCGGCAGCGTCCACTCATGCCGCTTCGTCT | 60.4 | 0.01 |
| mm4_exon_Gm20421/Hes5_chr4_154961383_R | GTCTCGTGGGCTCGGAGGAACTGTACCGCCTCCT | 59.9 | 0.01 |
| mm4_exon_Hipk1_chr3_103791518_F | TCGTCGGCAGCGTCACCGGTTTCCCAGTCCTCT | 60.1 | 0.01 |
| mm4_exon_Hipk1_chr3_103791518_R | GTCTCGTGGGCTCGGACTGTAAGAAACCCGACCGC | 60.3 | 0.01 |
| mm4_intergenic_Sbspon|4930444P10Rik_chr1_15965290_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Sbspon|4930444P10Rik_chr1_15965290_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Emilin2_chr17_71310954_F | TCGTCGGCAGCGTCCTCTGGCTCTTCGTCCCCA | 60.9 | 0.01 |
| mm4_exon_Emilin2_chr17_71310954_R | GTCTCGTGGGCTCGGGAGTCACGTTTCGGAGAGGG | 60.1 | 0.01 |
| mm3_intron_Rab8b_chr9_66919376_F | TCGTCGGCAGCGTCCATCCCCATCCGTCCCTTTC | 60.1 | 0.01 |
| mm3_intron_Rab8b_chr9_66919376_R | GTCTCGTGGGCTCGGCTTGGCGACCGGAGAAGG | 60.1 | 0.01 |
| mm4_intergenic_Hoxd11|Hoxd10_chr2_74687856_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Hoxd11|Hoxd10_chr2_74687856_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313685 |
CGCATCACAAGGTGACCCTAGCTCCCCACTGCCATCTCCGCGGTCGCCGTCGACACGGCGCTGGGGCTACCCGGCGCCTG CCTTGTCGCCTTAGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAG |
| mm4_intergenic_D630032N06Rik|Gm22587_chr11_85297035 |
CAGCTCTTAGGCCTCATGGGCTTCTGGTTCATTAATGAACCGATCCCTAAAACCACGGAGATGGAAGTGCGGACTGACCC AACACTGTCTGAGC |
| mm4_intergenic_Mir5112|4930445N18Rik_chr18_82839191 |
GCATTGTTTGGGCCACCTTCTTTCTTATCTTGATGGGCGGGGCCACGGAGAAGGCAGTGGGGCCTTTTCGTTTAAGTGTC CTTTCTAGATCTGGGTGAGGATATCTACCTGTGGAGCTCTGTCTTAATGGGC |
| mm4_intergenic_Rps16-ps3|3930402G23Rik_chr8_10884319 |
TTGCAGGGTGGTCTCATGACATTCTGCAGAGATTCTTGGCTATATGCAGGGTCTCACAACCATTTGCAAGGCCTCATTGC CATCTCCACAGTCTGATGACCTTTTGCAGAGTCTCATGGTTGTCTGCAGGGTC |
| mm4_exon_Angpt2_chr8_18699099 |
CTTTCCACGTCCTCTGGAAGTCCACACTGCCATCTTCTCGGTGTTGGATGACTGTCCACCCTCCTCCACCCACGTCCATG TCACAGTAGGCCTGCAGGAGAAGCACACAGTTACTGCAGGCTGAACATTGGT |
| mm4_intergenic_Moxd1|Gm15271_chr10_24411098 |
TCCATGCAGCAGATGTAGCAGAAGCATAGATAGCACTACCCACATGTTGTTGAGGAGATTCTGTGTAGTAAGTCGTGATG GCTGAGATGGCAGTGTGGGGCACCAGGATGTGCTGCTCGCTTAAGGCCTGGAAT |
| mm4_intron_Acaca_chr11_84358956 |
CGGTTTTCCCCTTCCCTCTCTCCCCTCAGCCCTTTCTTCTTGCCCGTCCTCCCTCTTGTCCCTTTATACCACTCTTTCCC CACTGCCATCTCATCTGTCTGTCTGTCTATCTGCCCTCCCCATCCTTTA |
| mm4_exon_4930513N10Rik/Cnot1_chr8_95806985 |
CGGGAGGAAAGGGACAGTTGAGGAGCTCGTGCGCTCCGAGGAGGCTGCAGCCCTGCGCGCGCGGCGAGCCGAGGCCCTGG GATCCGAACTGCCACCTCCGCCGCCGCCATCCTCGAAGCCTCCAG |
| mm4_exon_Igsf10_chr3_59341268 |
GTACAGTGCACCTCTGTGGGCACATAGCAGGCACATCGGCGAGGACAGACCCTGCTCCCAGGGGTGACCACCAGGCAGAT GGCAGTGAGGGAGATCAGCAAGCAGCTGACTTCCCTGCCTCTCTTCTGC |
| mm3_intron_Golt1b_chr6_142395347 |
GAGACTGAACGGCTGTTTGCCGTTTGAAGTGGGAAAACAGCAAAGACAGCAGAGATGGCAGTGAAGGTCAAAAGACCAGA GTTGACTCTTGATGCTGGGGTAGAATGAGGTGCTGTCATTTGTGCG |
| mm4_intergenic_Fitm2/2310001K24Rik|R3hdml_chr2_163487339 |
TGTGCATATGAAGTCATCAAAGGATAAAAATCTCAATGCTGTGAAACCGGGGAGATGGTAGTGAGGTTAAGAGCATTTGT GGCTTACAACCATCTATAACTCTTCTTCCCAGAGACCTTCACACA |
| mm4_exon_Hspa12b_chr2_131142668 |
CAGGAGACCGCTACATGGTGGCCGACTGCGGGGGTGGCACTGTGGACCTGACGGTACACCAGTTGGAGCAGCCTCACGGG ACCCTCAAGGAGCTCTA |
| mm4_intron_Bcat1_chr6_145017354 |
TAACAGCTTCACGCATGCACACTGCTCAGAGTTTGTTCTGGGTTAACCTCCCTGCCATCTCCCTGGTCTACACCATTCAC ACTTCACAGGGTTTAATTCCAAATATTAGAGAATGCAGTCAGAGATGGCAAGA |
| mm4_intron_Rere_chr4_150430612 |
CCCTCCAGCTGATGTGAATGTCAAAAATAATTGCTCCCACACTGCCATGTCCGGTGTGGCAGAGGGCAGTGCTCTGCTCT TCTTTCTGAAGAAAATATTTGGTTTACTTCATCCCATATTTGATTGGCTGCCTTGGTT |
| mm4_exon_4430402I18Rik/Ppapdc2_chr19_28963903 |
GTGTCACAATCGTGCTCTGCGGCTCGGCTCCTCGGCCCGCCCTCCGGGATGGCAGTGCGGAAGCGGAAGAGGCTGACGGG CTGGGAAGCCTGTCTCCGGTCTG |
| mm4_intron_Rgs7_chr1_175464036 |
CCAGCTGCCCCTGAAGTTATCTATCCCAACCTCTGACCACTAGGCCACCTTGCCATCTCCCCGGTCTGGTTCTTGTGTGT AGCACATGTTTCTGTGCCTTTTTACAGCGTGGAGCTTACAAAACCT |
| mm4_intron_Pcsk2_chr2_143620642 |
AATAGCAGAAAGGACCACTAGGCCACCTTGCCATCTCCCCGGTCTGGTTCTTGTGTGTNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNTAGCAGAAAGGCTGTGGCA |
| mm4_intergenic_Gm13002|Wnt4_chr4_137251517 | AAGACAAGGGTGCTGACTGGAGGTTCCGACCGCAGAGCTGATAGTGAGGTCGGGCAAAGTGACCCCTACACT |
| mm4_intergenic_Gm15387|Bai1_chr15_74407439 |
AAGAAGCACTCTGTCCAGGGGCTGCAACTGGGGGAGGGGAGGCAGCTACAGAGGAGAGGGCAGTGGGGCGGGCACGGGCC ACACCAATGGTAGCCGCTA |
| mm4_intron_Tcf7l2_chr19_55806328 |
CGAGGCCAGGTTCTATGGTCATGGCTAGTTGGCATCACAAATAAACCAACTCCTGGAAAATTCTCTTACTGTCAATAGTG AAGACCGGGGGGAGGGCAGTGGGGGAGAGGAAGAACTGATCTTGATGCTGTTGGGCT |
| mm4_exon_Gm10322_chr10_59616362 |
GGAGAGGTTCAAGTCCCGTGCCTTCCCCGCCCTGGCGTCCCCAGTCCTGCGGGAACATCCCTGCTGTCGCCCCTCCTCCT CCTCTCCTGCCTCCCGCCCGCCCTGCCTTCTCCGCCGCCGCCGCAGGCGCTGAAGGACAC |
| mm4_intergenic_Cacna2d3|Selk_chr14_29848054 |
TGGGCTTCATCAGTTGCTTCTAAATCTGAACCCAAACAGAGTGAGCTGAAAAGGTATTTTCAAGGACTAATCATACCGTG GCATCAGAGCTACCACGGAGATGTCAATGAGGACAGAGCCCAAGAGTTGTTCGG |
| mm4_intron_Pgpep1_chr8_70656615 |
CTGAGACACACTGGGGCTTTTTGTCTGGTGGTCCCGGGAGTGGATGCACAAGCAGGGGCCCGGCCACACTCCCATCTGCG AGGTCCCTGGGACCCCGGGGTCCTCCACCTGGCTCACAGAGTGAAGG |
| mm4_exon_H2-DMa_chr17_34138044 |
CTCTTTCCACCGACCCTGACCGTGAACTGGCAGCTTCACTCGGCCCCTGTGGAGGGAGCCAGCCCCACATCCATCTCAGC TGTCGATGGGCTGACCTTCCAGGCTTTCT |
| mm4_exon_Ust_chr10_8206082 |
TCTTCAATGGCAGGCTCTGGGGTAAACCTGAGGCAGACACAAGTTGTCACCAAATGTCCTTCCCACTGCCATCACTGCAG TCTGGCCTCTCTAGTTGCATTCT |
| mm4_intergenic_Gpm6a|Adam29_chr8_55376527 |
AGCACCTCCCCAGAAGAGAAGCCCCAGGAGCAAGCATCCCAGGAAGTCCGCGGAGATGGGAGAGAGGTTTTCCTTGGGCT TTGCAGC |
| mm4_intergenic_Gpm6a|Adam29_chr8_55366648 |
TTGGAGAAGGGCCTCACAACGGAGGGCCAGAGCACCTCCCCAGAAGAGAAGCCCCAGGAGCAAGCATCCCAGGAAGTCCG CGGAGATGGGAGAGAGGTTTTCCTTGGGCTTTGCAGC |
| mm4_intergenic_Gpm6a|Adam29_chr8_55360015 |
TTGGAGAAGGGCCTCACAACGGAGGGCCTGAGCACCTCCCCAGAAGAGAAGCCCCAGGAGCAAGCATCCCAGGAAGTCCG CGGAGATGGGAGAGAGGTTTTCCTTGGGCTTTGCAGC |
| mm4_intergenic_Gpm6a|Adam29_chr8_55113182 |
AGCACCTCCCCAGAAGAGAAGCCCCAGGAGCAAGCATCCCAGGAAGTCCGCGGAGATGGGAGAGAGGTTTAACTTGGGCT TTGCAGCTGCTGTCCAGGCAGCAGCTCTTGCCTAAGCCAGAGCCGAAAATCGCACCAAA |
| mm4_intron_Ano1_chr7_144622615 | TCTAGGGGATGCAGGTGACCCACACACACACTCCCCACTCCCAGCTCCGCTGTCCATACAAACCCAGTGTCAGAACA |
| mm4_intergenic_Gm13652|A330043C09Rik_chr2_75143949 |
ATGGCTACAAGGTGACCGACCGACCGCAGGGCTGGCTGTGGGGGTCAGAGGGAAAGCTGTTCTCTCTCTTTTCCTTGTGA CTGCTCCCTTGTTTATTCTTTGCTTGCTGTACTTCCCTCG |
| mm4_exon_Gm20421/Hes5_chr4_154961383 |
CACTCATGCCGCTTCGTCTCCCCACAGCCTTCGCCGCGGCCGCCGGCCCCAAGAGCCTGCACCAGGACTACAGCGAGGGC TACTCCTGGTGCCTGCAGGAGGCGGTACAGTTCCT |
| mm4_exon_Hipk1_chr3_103791518 |
ACCGGTTTCCCAGTCCTCTCCGCACAGCAGTCTCCGAGGTCGTTTAACCGGCGTTTGGTGGCGGTCGGGTTTCTTACAGT |
| mm4_exon_Emilin2_chr17_71310954 |
CTCTGGCTCTTCGTCCCCACGCCTCCGCACTGGCCGCTCCGCGGTCCCTCAATGACGCACAGGTCCCCCCGGGTGCCGCC CTCTTTAATTCACTTCCCTCTCCGAAACGTGACTC |
| mm3_intron_Rab8b_chr9_66919376 |
CATCCCCATCCGTCCCTTTCAGCCTCTCCGGCTCTCACTGCCCTCTCCGCGGCCCTTCGGGGTCCCCTTCTCCGGTCGCC AAG |