CRISPOR

DNA break repair outcome predictions

Guide: ATGGCCGCTCCAGCAGACCG TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTGGGGGTCCCGGGCCAAGCATGGCCGCTCCAGCAGACCGTGGCTCGGCGCCCAGCCCTGCAGCACTGCCTGCTAGTCCA`	(Wild-type sequence)
314	`CGGGCCAAGCATGGCCGCTC----------------GGCGCCCAGCCCTGCAGCACTGCC`	`16 bp deleted → frameshift`
299	`CGGGCCAAGCATGGCCGCTCCAGC----------------------CCTGCAGCACTGCC`	`22 bp deleted → frameshift`
282	`CGGGCCAAGCATGGCCGCTCCAGCAGACCG-------GCGCCCAGCCCTGCAGCACTGCC`	`7 bp deleted → frameshift`
270	`CGGGCCAAGCATGGC-------------------TCGGCGCCCAGCCCTGCAGCACTGCC`	`19 bp deleted → frameshift`
242	`CGGGCCAAGCATGGCCGCTCCAGCAGACCG----------CCCAGCCCTGCAGCACTGCC`	`10 bp deleted → frameshift`
242	`CGGGCCAAGCATGGCCGCTCCAGC----------TCGGCGCCCAGCCCTGCAGCACTGCC`	`10 bp deleted → frameshift`
208	`CGGGCCAAGCATGGCCGCTCCAGCAGACC-------------CAGCCCTGCAGCACTGCC`	`13 bp deleted → frameshift`
198	`CGGGCCAAGCATGGCCGCTCCAGCAGACC--------------AGCCCTGCAGCACTGCC`	`14 bp deleted → frameshift`
193	`CGGGCCAAGCATGGCCGCTCCAGCAG-------------------CCCTGCAGCACTGCC`	`19 bp deleted → frameshift`
190	`CGGGCCAAGCATGGCCGC-----------------------CCAGCCCTGCAGCACTGCC`	`23 bp deleted → frameshift`
188	`CGGGCCAAGCATGGCCGCTCCAGC---------------GCCCAGCCCTGCAGCACTGCC`	`15 bp deleted → no frameshift`
181	`CGGGCCAAGCATGGCCGCTCCAGCAG---------------------------CACTGCC`	`27 bp deleted → no frameshift`
180	`CGGGCCAAGCATGGC------------------------GCCCAGCCCTGCAGCACTGCC`	`24 bp deleted → no frameshift`
178	`CGGGCCAAGCATGGCCGCTCCAGCA------------------------------CTGCC`	`30 bp deleted → no frameshift`
170	`CGGGCCAAGCATGGCCGCTCCAGC-----------------CCAGCCCTGCAGCACTGCC`	`17 bp deleted → frameshift`
163	`CGGGCCAAGCATGGCC--------------------------CAGCCCTGCAGCACTGCC`	`26 bp deleted → frameshift`
162	`CGGGCCAAGCATGGCCGCTCCAGCAGACC------------------CTGCAGCACTGCC`	`18 bp deleted → no frameshift`
154	`CGGGCCAAGCATGGCCGCTCCAGCAGACC-------------------TGCAGCACTGCC`	`19 bp deleted → frameshift`
147	`CGGGCCAAGCATGGCCG--------------------GCGCCCAGCCCTGCAGCACTGCC`	`20 bp deleted → frameshift`
141	`CGGGC-----------------------------TCGGCGCCCAGCCCTGCAGCACTGCC`	`29 bp deleted → frameshift`
140	`CGGGCCAAGCATGGCCGCTCC---------------------CAGCCCTGCAGCACTGCC`	`21 bp deleted → no frameshift`
140	`CGGGCCAAGCATGGCCGC---------------------GCCCAGCCCTGCAGCACTGCC`	`21 bp deleted → no frameshift`
127	`CGGGCCAAGCATGGCC-------------------------------CTGCAGCACTGCC`	`31 bp deleted → frameshift`
120	`CGGGCCAAGC------------------------TCGGCGCCCAGCCCTGCAGCACTGCC`	`24 bp deleted → no frameshift`
109	`CGGGC----------------------------------GCCCAGCCCTGCAGCACTGCC`	`34 bp deleted → frameshift`
109	`CGGGCCAAGCATGGCCGCTCC--------------------------CTGCAGCACTGCC`	`26 bp deleted → frameshift`
104	`CGG-----------------------------------CGCCCAGCCCTGCAGCACTGCC`	`35 bp deleted → frameshift`
103	`CGGGCCAAGCATGGCCGCTCC---------------------------TGCAGCACTGCC`	`27 bp deleted → no frameshift`
103	`CGGGCCAAGCATGGCC---------------------------AGCCCTGCAGCACTGCC`	`27 bp deleted → no frameshift`
99	`CGGGCC------------------------------------CAGCCCTGCAGCACTGCC`	`36 bp deleted → no frameshift`
98	`CGGGCCAAGCATGGCCGC----------------------------CCTGCAGCACTGCC`	`28 bp deleted → frameshift`
94	`CGGGCCAAGC-----------------------------GCCCAGCCCTGCAGCACTGCC`	`29 bp deleted → frameshift`
89	`CGG------------------------------CTCGGCGCCCAGCCCTGCAGCACTGCC`	`30 bp deleted → no frameshift`
84	`CGGGCCAAGCATGGCCGCTCCAGCAGACC-------------------------------`	`31 bp deleted → frameshift`
84	`CGGGCCAAGC-------------------------------CCAGCCCTGCAGCACTGCC`	`31 bp deleted → frameshift`
82	`CGGGCCAAGC------------------------------------CCTGCAGCACTGCC`	`36 bp deleted → no frameshift`
80	`CGGGCCAAGCATGGCC--------------------------------TGCAGCACTGCC`	`32 bp deleted → frameshift`
78	`CGGGCCA-------------------------------------GCCCTGCAGCACTGCC`	`37 bp deleted → frameshift`
77	`CGGGCC-----------------------------------------CTGCAGCACTGCC`	`41 bp deleted → frameshift`
76	`CGGGCCAAGCATGGCCGC---------------------------------AGCACTGCC`	`33 bp deleted → no frameshift`
74	`CGGGCCAAGCATGGCCGCTCCAGCAGAC----------------------------TGCC`	`28 bp deleted → frameshift`
69	`CGGGCCAAGCATGGCCGCTCCAGC-----------------------------------C`	`35 bp deleted → frameshift`
66	`CGGGCCAAGCATGGCCGCT------------------------------GCAGCACTGCC`	`30 bp deleted → no frameshift`
66	`CGGGCCAAGCATGGCC--------------------------------------------`	`44 bp deleted → frameshift`
66	`CGGGCCAAGCA--------------------------------------------CTGCC`	`44 bp deleted → frameshift`
66	`CGGGCCAAGCATGGCCGC------------------------------------ACTGCC`	`36 bp deleted → no frameshift`
66	`CGGGCCAAGCATGGC------------------------------------AGCACTGCC`	`36 bp deleted → no frameshift`
64	`CGGGCCAAGCA-----------------------------------------GCACTGCC`	`41 bp deleted → frameshift`
60	`CG--------------------------------------CCCAGCCCTGCAGCACTGCC`	`38 bp deleted → frameshift`
57	`CGGGCCAAGCATGGCCGCTCCA---------------------------------CTGCC`	`33 bp deleted → no frameshift`
57	`CGGGCCAAGCA---------------------------------GCCCTGCAGCACTGCC`	`33 bp deleted → no frameshift`
56	`CGGGCCAAGCATGGCCGCTCC---------------------------------------`	`39 bp deleted → no frameshift`
56	`CGGGCCAAGCATGGC---------------------------------------ACTGCC`	`39 bp deleted → no frameshift`
51	`CGGGCCAAGCATGGCCGC-----------------------------------------C`	`41 bp deleted → frameshift`
48	`CGGGCC------------------------------------------TGCAGCACTGCC`	`42 bp deleted → no frameshift`
47	`CGGGCCAAGCATG-------------------------------------CAGCACTGCC`	`37 bp deleted → frameshift`
45	`CGGGCCAAGCATGGCCGCT--------------------------------------GCC`	`38 bp deleted → frameshift`
40	`CGGGCC------------------------------------------------------`	`54 bp deleted → no frameshift`
40	`CGGGC----------------------------------------------AGCACTGCC`	`46 bp deleted → frameshift`
34	`CGGGCCAAGC-------------------------------------------------C`	`49 bp deleted → frameshift`
34	`CGGGC-------------------------------------------------ACTGCC`	`49 bp deleted → frameshift`
31	`CGGGCCAAGCATG---------------------------------------------CC`	`45 bp deleted → no frameshift`
31	`CGGGCCA---------------------------------------------GCACTGCC`	`45 bp deleted → no frameshift`
27	`CGGGCCA------------------------------------------------CTGCC`	`48 bp deleted → no frameshift`

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