CRISPOR

DNA break repair outcome predictions

Guide: CCCCGGACTCTCCAGAGGGG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ATGTCCTTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGGAAAACCCCCAAGTAAGCTCCTAATCCCAGGC`	(Wild-type sequence)
737	`GGCTCTCCATCCCCGGACTCTCCAGAGGGG--------GAAAACCCCCAAGTAAGCTCCT`	`8 bp deleted → frameshift`
516	`GGCTCTCCATCCCCGGACTCTCCAGAGGGG---AGGGGGAAAACCCCCAAGTAAGCTCCT`	`3 bp deleted → no frameshift`
510	`GGCTCTCCATCCCCGGACTCTCCAGAGGGG---------AAAACCCCCAAGTAAGCTCCT`	`9 bp deleted → no frameshift`
491	`GGCTCTCCATCCCCGGACTCTCCAGAGGG----AGGGGGAAAACCCCCAAGTAAGCTCCT`	`4 bp deleted → frameshift`
423	`GGCTCTCCATCCCCGGACTCTCCAGAGGGG-------GGAAAACCCCCAAGTAAGCTCCT`	`7 bp deleted → frameshift`
364	`GGCTCTCCATCCCCGGACTCTCCAGAGGG----------AAAACCCCCAAGTAAGCTCCT`	`10 bp deleted → frameshift`
362	`GGCTCTCCATCCCCGGACTCTCCAGAGGGG--GAGGGGGAAAACCCCCAAGTAAGCTCCT`	`2 bp deleted → frameshift`
311	`GGCTCTCCATCCCCGGACTCTCCAGAGG-----AGGGGGAAAACCCCCAAGTAAGCTCCT`	`5 bp deleted → frameshift`
296	`GGCTCTCCATCCCCGGACTCTCCAGAGGGG------GGGAAAACCCCCAAGTAAGCTCCT`	`6 bp deleted → no frameshift`
230	`GGCTCTCCATCCCCGGACTCTCCAGAGG-----------AAAACCCCCAAGTAAGCTCCT`	`11 bp deleted → frameshift`
213	`GGCTCTCCATCCCCGGA-----------------GGGGGAAAACCCCCAAGTAAGCTCCT`	`17 bp deleted → frameshift`
182	`GGCTCTCCATCCCCGGACTCTCCAG----------GGGGAAAACCCCCAAGTAAGCTCCT`	`10 bp deleted → frameshift`
179	`GGCTCTCCATCCCCGG----------------GAGGGGGAAAACCCCCAAGTAAGCTCCT`	`16 bp deleted → frameshift`
158	`GGCTCTCCATCCCCGGA-----------------------AAACCCCCAAGTAAGCTCCT`	`23 bp deleted → frameshift`
153	`GGCTCTCCATCCCC---------------------------------CAAGTAAGCTCCT`	`33 bp deleted → no frameshift`
149	`GGCTCTCCATCCCCGGACTCTCCAGA--------------AAACCCCCAAGTAAGCTCCT`	`14 bp deleted → frameshift`
147	`GGCTCTCCATCCCCGG--------------------GGGAAAACCCCCAAGTAAGCTCCT`	`20 bp deleted → frameshift`
146	`GGCTCTCCATCCCC----------------------------------AAGTAAGCTCCT`	`34 bp deleted → frameshift`
143	`GGCTCTCCATCCCCGGACTCTCCA-------------------------AGTAAGCTCCT`	`25 bp deleted → frameshift`
140	`GGCTCTCCATCCCCGG---------------------GGAAAACCCCCAAGTAAGCTCCT`	`21 bp deleted → no frameshift`
133	`GGCTCTCCATCCCCGGACTCTCC----------------------CCCAAGTAAGCTCCT`	`22 bp deleted → frameshift`
133	`GGCTCTCCATCCCCGG----------------------GAAAACCCCCAAGTAAGCTCCT`	`22 bp deleted → frameshift`
126	`GGCTCTCCATCCCCGGACTCTCC-----------------------CCAAGTAAGCTCCT`	`23 bp deleted → frameshift`
121	`GGCTCTCCATCCCC--------------------------------CCAAGTAAGCTCCT`	`32 bp deleted → frameshift`
120	`GGCTCTCCATCCCCGGACTCTCC------------------------CAAGTAAGCTCCT`	`24 bp deleted → no frameshift`
115	`GGCTCTCCATCCCCGGACTCTCC------------------------------------T`	`36 bp deleted → no frameshift`
104	`GGCTCTCCATCCC-----------------------------------AAGTAAGCTCCT`	`35 bp deleted → frameshift`
90	`GGCTCTCCATCCCCGGACTCTCCAGAG------------------------TAAGCTCCT`	`24 bp deleted → no frameshift`
89	`GG------------------------------GAGGGGGAAAACCCCCAAGTAAGCTCCT`	`30 bp deleted → no frameshift`
84	`GGCTCTCCATCCCC-------------------------------CCCAAGTAAGCTCCT`	`31 bp deleted → frameshift`
84	`GG-------------------------------AGGGGGAAAACCCCCAAGTAAGCTCCT`	`31 bp deleted → frameshift`
81	`GGCTCTCCATCCCCGGACTCTCCAG--------------------------TAAGCTCCT`	`26 bp deleted → frameshift`
81	`GGCTCTCCATCCCCGGAC--------------------------CCCCAAGTAAGCTCCT`	`26 bp deleted → frameshift`
75	`GGCTCTCCATCCCCGGACTC--------------------------------------CT`	`38 bp deleted → frameshift`
74	`GGCTCTCCATCCCCGGACTCTCCAGAG----------------------------CTCCT`	`28 bp deleted → frameshift`
73	`GG----------------------------------GGGAAAACCCCCAAGTAAGCTCCT`	`34 bp deleted → frameshift`
69	`GG-----------------------------------GGAAAACCCCCAAGTAAGCTCCT`	`35 bp deleted → frameshift`
67	`GGCTCTCCA----------------------------------------AGTAAGCTCCT`	`40 bp deleted → frameshift`
66	`GGCTCTCCATCCCCGGACTCTCCAG------------------------------CTCCT`	`30 bp deleted → no frameshift`
66	`GGCTCTCCATCC------------------------------------AAGTAAGCTCCT`	`36 bp deleted → no frameshift`
66	`GG------------------------------------GAAAACCCCCAAGTAAGCTCCT`	`36 bp deleted → no frameshift`
62	`GGCTCTCC-------------------------------------CCCAAGTAAGCTCCT`	`37 bp deleted → frameshift`
62	`GG-------------------------------------AAAACCCCCAAGTAAGCTCCT`	`37 bp deleted → frameshift`
60	`GGCTCTCC--------------------------------------CCAAGTAAGCTCCT`	`38 bp deleted → frameshift`
56	`GGCTCTCC---------------------------------------CAAGTAAGCTCCT`	`39 bp deleted → no frameshift`
54	`GGCTCTCC---------------------------------------------------T`	`51 bp deleted → no frameshift`
49	`GGCTC-----------------------------------------------------CT`	`53 bp deleted → frameshift`
47	`GGCTCTCCATCC-----------------------------------------------T`	`47 bp deleted → frameshift`
42	`GGCTCTCCATCCCCGGACTCT---------------------------------------`	`39 bp deleted → no frameshift`
42	`GGCTCTCCATCCCC---------------------------------------------T`	`45 bp deleted → no frameshift`
40	`GGCTCTCCATCCC----------------------------------------------T`	`46 bp deleted → frameshift`
38	`GGCTCTCCATCCCCGGACT-----------------------------------------`	`41 bp deleted → frameshift`
20	`GGCTCT------------------------------------------------------`	`54 bp deleted → no frameshift`
18	`GGCT--------------------------------------------------------`	`56 bp deleted → frameshift`

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