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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313995_F | TCGTCGGCAGCGTCAACCGCCAGAGAAGATGGTG | 60.0 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313995_R | GTCTCGTGGGCTCGGCTGCCTGCTAGTCCATCGAC | 60.2 | 1.00 |
| mm4_intron_Tmem241_chr18_12118181_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intron_Tmem241_chr18_12118181_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intron_Pik3r1_chr13_101752595_F | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intron_Pik3r1_chr13_101752595_R | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intergenic_Gm25522|Gm25587_chr10_104966539_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_Gm25522|Gm25587_chr10_104966539_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_2610028E06Rik|Csf3r_chr4_125967458_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intergenic_2610028E06Rik|Csf3r_chr4_125967458_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intron_1110051M20Rik_chr2_91299497_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intron_1110051M20Rik_chr2_91299497_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_Gm12947|4930471C06Rik_chr4_127433590_F | TCGTCGGCAGCGTCGTTGGTTGCTGTTGTGGACG | 60.2 | 0.29 |
| mm4_intergenic_Gm12947|4930471C06Rik_chr4_127433590_R | GTCTCGTGGGCTCGGGTATGGGCAGATGGGACTGG | 59.8 | 0.29 |
| mm4_exon_Slc46a2_chr4_59914869_F | TCGTCGGCAGCGTCCCACCACAGGCTCAATCCAA | 60.2 | 0.28 |
| mm4_exon_Slc46a2_chr4_59914869_R | GTCTCGTGGGCTCGGCAAGCCCTGTTCCCTGTCAC | 60.8 | 0.28 |
| mm4_intron_Sipa1l2_chr8_125458507_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Sipa1l2_chr8_125458507_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Ptprj|Nup160_chr2_90581118_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Ptprj|Nup160_chr2_90581118_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_exon_Gja10_chr4_32601087_F | TCGTCGGCAGCGTCCAGAGACCGTGAGCTTCCTG | 60.1 | 0.24 |
| mm4_exon_Gja10_chr4_32601087_R | GTCTCGTGGGCTCGGCAGTGCAAGAGAGAGTGGGG | 60.0 | 0.24 |
| mm4_intergenic_Ehd1|Cdc42bpg_chr19_6306393_F | TCGTCGGCAGCGTCCCCGCCCAGGAGTTACTTTT | 59.9 | 0.23 |
| mm4_intergenic_Ehd1|Cdc42bpg_chr19_6306393_R | GTCTCGTGGGCTCGGGCTGAAGGTGAAGGTGGGAA | 59.8 | 0.23 |
| mm4_intergenic_Gm22438|Gm22159_chr1_109584739_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm22438|Gm22159_chr1_109584739_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Jdp2|Batf_chr12_85658074_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Jdp2|Batf_chr12_85658074_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm3_intergenic_Gm26993|Tle4_chr19_14431277_F | TCGTCGGCAGCGTCGCAGCCTACAAGCCACAAGA | 60.6 | 0.21 |
| mm3_intergenic_Gm26993|Tle4_chr19_14431277_R | GTCTCGTGGGCTCGGTGGAGCCACATAAGAATCAGGA | 58.8 | 0.21 |
| mm4_intergenic_Gm14254|Tox2_chr2_163166852_F | TCGTCGGCAGCGTCCCAGCCTTGCAACTCTGAGA | 59.9 | 0.21 |
| mm4_intergenic_Gm14254|Tox2_chr2_163166852_R | GTCTCGTGGGCTCGGACCCAAGGGCCAGAGTAAGA | 60.1 | 0.21 |
| mm4_intron_2610307P16Rik_chr13_28628562_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_2610307P16Rik_chr13_28628562_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Rrm1_chr7_102461706_F | TCGTCGGCAGCGTCGGACTGGAAGCCTCTCAAGG | 59.7 | 0.20 |
| mm4_intron_Rrm1_chr7_102461706_R | GTCTCGTGGGCTCGGAGTTTCAGAGGTCACACTAGCA | 59.0 | 0.20 |
| mm4_exon_Aaas_chr15_102350500_F | TCGTCGGCAGCGTCATTCCCACTTTCCGCATCCC | 60.3 | 0.19 |
| mm4_exon_Aaas_chr15_102350500_R | GTCTCGTGGGCTCGGAATAACGAGCTGGTGACCGG | 60.1 | 0.19 |
| mm4_intergenic_Gm22609|Lrrn2_chr1_132849257_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm22609|Lrrn2_chr1_132849257_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm25288|Cep170_chr1_176803311_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm25288|Cep170_chr1_176803311_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Top2a_chr11_99002403_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Top2a_chr11_99002403_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Ctc1|Aurkb_chr11_69040216_F | TCGTCGGCAGCGTCGAAGAGACGAGGGGACGACC | 61.6 | 0.16 |
| mm4_intergenic_Ctc1|Aurkb_chr11_69040216_R | GTCTCGTGGGCTCGGGTGACACCACTCCTTGAGGG | 59.9 | 0.16 |
| mm4_intergenic_Boll|Plcl1_chr1_55406171_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Boll|Plcl1_chr1_55406171_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm23795|Gm5342_chr7_68974983_F | TCGTCGGCAGCGTCGTCAGGATGGGTCGGTCAAT | 59.4 | 0.15 |
| mm4_intergenic_Gm23795|Gm5342_chr7_68974983_R | GTCTCGTGGGCTCGGCTGAGAAGGTGAGTGGAGGG | 59.0 | 0.15 |
| mm4_exon_Tsc22d3_chrX_140542587_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Tsc22d3_chrX_140542587_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Arap3|Pcdh1_chr18_38041342_F | TCGTCGGCAGCGTCTCCTATGGGACTGCCTTCCA | 59.9 | 0.13 |
| mm4_intergenic_Arap3|Pcdh1_chr18_38041342_R | GTCTCGTGGGCTCGGACCTGCCCTCATCCCTATGT | 60.0 | 0.13 |
| mm4_intron_Tcf25_chr8_123396516_F | TCGTCGGCAGCGTCGCTGCAGAGTAAGCACGTCT | 60.3 | 0.13 |
| mm4_intron_Tcf25_chr8_123396516_R | GTCTCGTGGGCTCGGTCCGAACTGCTCTCATGCTG | 60.1 | 0.13 |
| mm4_intergenic_Gm26626|Runx1_chr16_92634511_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm26626|Runx1_chr16_92634511_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm14275|Gm14273_chr2_172311968_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm14275|Gm14273_chr2_172311968_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Fzd10|Piwil1_chr5_128670251_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Fzd10|Piwil1_chr5_128670251_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Mecom_chr3_30515625_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Mecom_chr3_30515625_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Hipk2_chr6_38726659_F | TCGTCGGCAGCGTCACTTGAAGGTTTCTAACACTAGAGA | 57.4 | 0.10 |
| mm4_intron_Hipk2_chr6_38726659_R | GTCTCGTGGGCTCGGGAGTGGAACCAACAGGCTCA | 59.8 | 0.10 |
| mm4_intergenic_Gm12443|Anp32b_chr4_46450739_F | TCGTCGGCAGCGTCCGAAGGCTCAGGCGAAATTG | 59.9 | 0.10 |
| mm4_intergenic_Gm12443|Anp32b_chr4_46450739_R | GTCTCGTGGGCTCGGATGTAAGACCTTCCGGTGCC | 59.7 | 0.10 |
| mm4_exon_Cdca7_chr2_72483890_F | TCGTCGGCAGCGTCATTTGGGGATGCAGAGAGCC | 60.1 | 0.09 |
| mm4_exon_Cdca7_chr2_72483890_R | GTCTCGTGGGCTCGGGTCGGATTATATGCGGAAGGGT | 60.0 | 0.09 |
| mm4_intron_Fam179a_chr17_71710572_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Fam179a_chr17_71710572_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Cntn4_chr6_106688329_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Cntn4_chr6_106688329_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Zfp711_chrX_112600835_F | TCGTCGGCAGCGTCGAACGCCAGGACCTTCCAG | 60.3 | 0.08 |
| mm4_exon_Zfp711_chrX_112600835_R | GTCTCGTGGGCTCGGGCTCAGGAGTCCCCTTACCT | 60.3 | 0.08 |
| mm4_intergenic_Sorbs1|Aldh18a1_chr19_40495825_F | TCGTCGGCAGCGTCGCAGACAAGCAAACAAGGGAG | 60.0 | 0.08 |
| mm4_intergenic_Sorbs1|Aldh18a1_chr19_40495825_R | GTCTCGTGGGCTCGGGCAGGGGTAGAGAGGAAGGA | 60.0 | 0.08 |
| mm4_intergenic_Dhrs7|Ppm1a_chr12_72761110_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Dhrs7|Ppm1a_chr12_72761110_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Cdkal1|Gm11364_chr13_29210588_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Cdkal1|Gm11364_chr13_29210588_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Pkd2_chr5_104459987_F | TCGTCGGCAGCGTCCGACGAGGTGGAAGGAGAAG | 59.8 | 0.07 |
| mm4_exon_Pkd2_chr5_104459987_R | GTCTCGTGGGCTCGGGAGCTCACGGCCGAGGAG | 62.2 | 0.07 |
| mm4_exon_D10Bwg1379e_chr10_18625211_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_D10Bwg1379e_chr10_18625211_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Lgr6_chr1_135021547_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intron_Lgr6_chr1_135021547_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm24051|Gm11988_chr11_8047070_F | TCGTCGGCAGCGTCATCACTGGAATGTGCAGGCC | 60.6 | 0.06 |
| mm4_intergenic_Gm24051|Gm11988_chr11_8047070_R | GTCTCGTGGGCTCGGACACACCTTAGACAGAGGAGAC | 58.8 | 0.06 |
| mm4_exon_Kctd15_chr7_34641992_F | TCGTCGGCAGCGTCCGATCCGTTCACCCAAGTCA | 60.0 | 0.06 |
| mm4_exon_Kctd15_chr7_34641992_R | GTCTCGTGGGCTCGGGCAAGGTACTACCAGCTGCA | 60.0 | 0.06 |
| mm4_exon_Gm12979_chr4_129491987_F | TCGTCGGCAGCGTCTGGCCGTGTATGTGTATCCG | 59.8 | 0.06 |
| mm4_exon_Gm12979_chr4_129491987_R | GTCTCGTGGGCTCGGTGGAGGTAGCCCAAGAGTGA | 59.8 | 0.06 |
| mm4_intron_Ttbk1_chr17_46468874_F | TCGTCGGCAGCGTCCTGCATGGCAACTGTCTAAGC | 59.8 | 0.06 |
| mm4_intron_Ttbk1_chr17_46468874_R | GTCTCGTGGGCTCGGTACCCCTGGGAAACGAAACG | 59.9 | 0.06 |
| mm4_intergenic_Gm24444|Mgmt_chr7_136878090_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm24444|Mgmt_chr7_136878090_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Ppapdc3_chr2_32096314_F | TCGTCGGCAGCGTCGGCCATCTGTCTGTCACCC | 60.0 | 0.06 |
| mm4_intron_Ppapdc3_chr2_32096314_R | GTCTCGTGGGCTCGGAGGCTTGTCCATTTCTGGGG | 59.9 | 0.06 |
| mm4_intergenic_Mvk|Gm13790_chr5_114527098_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Mvk|Gm13790_chr5_114527098_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Mapk8ip3_chr17_24902252_F | TCGTCGGCAGCGTCAAAAGGAAGAGCTACCCGCC | 60.0 | 0.05 |
| mm4_exon_Mapk8ip3_chr17_24902252_R | GTCTCGTGGGCTCGGCGTCCTGTGTATCTCCAGCA | 59.1 | 0.05 |
| mm4_intergenic_Gm27027|4921507L20Rik/Alkbh3_chr2_93976559_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm27027|4921507L20Rik/Alkbh3_chr2_93976559_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Abcd3_chr3_121811080_F | TCGTCGGCAGCGTCGGTGACCCTTCTCTAGCCCT | 60.3 | 0.05 |
| mm4_intron_Abcd3_chr3_121811080_R | GTCTCGTGGGCTCGGAGGCCAGTTTCTCCACTGTT | 59.1 | 0.05 |
| mm4_exon_Kcnab3_chr11_69326607_F | TCGTCGGCAGCGTCCGATCGCGCTATTACCACCA | 60.3 | 0.05 |
| mm4_exon_Kcnab3_chr11_69326607_R | GTCTCGTGGGCTCGGAAGGTTCTGCTCGGTACACG | 60.0 | 0.05 |
| mm4_exon_1810041L15Rik_chr15_84380507_F | TCGTCGGCAGCGTCAGGCAGACACTGGAACACAC | 60.1 | 0.04 |
| mm4_exon_1810041L15Rik_chr15_84380507_R | GTCTCGTGGGCTCGGAACCTGTGCAACCTCCTGAG | 59.8 | 0.04 |
| mm4_intergenic_Tbx4|Gm11443_chr11_85916924_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Tbx4|Gm11443_chr11_85916924_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Tesk1_chr4_43443523_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Tesk1_chr4_43443523_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Trnp1_chr4_133497229_F | TCGTCGGCAGCGTCCCGGGTATCTGTCTCCTCCA | 60.1 | 0.03 |
| mm4_intron_Trnp1_chr4_133497229_R | GTCTCGTGGGCTCGGCTTCTACACAGCCCAGCTCC | 60.1 | 0.03 |
| mm4_intron_Faah_chr4_116017366_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Faah_chr4_116017366_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Igsf1|n-R5s6_chrX_49798002_F | TCGTCGGCAGCGTCCCTGTCGCTCAGAGCTTCAT | 59.8 | 0.03 |
| mm4_intergenic_Igsf1|n-R5s6_chrX_49798002_R | GTCTCGTGGGCTCGGACCCGAGGTCGCTTTAAAGG | 60.0 | 0.03 |
| mm4_intron_Arhgap26_chr18_38993119_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Arhgap26_chr18_38993119_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_1700021F05Rik_chr10_43533576_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_1700021F05Rik_chr10_43533576_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm2_intron_Cit_chr5_115872638_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm2_intron_Cit_chr5_115872638_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Unc5cl|Gm24071_chr17_48557779_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Unc5cl|Gm24071_chr17_48557779_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cd63_chr10_128909668_F | TCGTCGGCAGCGTCCAGAGAGAACCAGACGGCG | 60.1 | 0.02 |
| mm4_intron_Cd63_chr10_128909668_R | GTCTCGTGGGCTCGGGAGTCACCAGGCTAGCAGG | 59.4 | 0.02 |
| mm4_intergenic_Cox6c|Rgs22_chr15_35984810_F | TCGTCGGCAGCGTCGAGACACATTCCGCACCAGA | 60.0 | 0.02 |
| mm4_intergenic_Cox6c|Rgs22_chr15_35984810_R | GTCTCGTGGGCTCGGAGCTTGCCTCCAGGTTTTCT | 59.5 | 0.02 |
| mm4_intergenic_Hoxd11|Hoxd10_chr2_74690095_F | TCGTCGGCAGCGTCACAGTTTCCGGGTCCGTTTT | 60.1 | 0.02 |
| mm4_intergenic_Hoxd11|Hoxd10_chr2_74690095_R | GTCTCGTGGGCTCGGTCCAAAGACTTGCCCTGGAC | 59.8 | 0.02 |
| mm4_intergenic_Gm23772|9130230N09Rik_chr11_22858804_F | TCGTCGGCAGCGTCTAGGCGAGTGCATTTGAGCA | 60.0 | 0.01 |
| mm4_intergenic_Gm23772|9130230N09Rik_chr11_22858804_R | GTCTCGTGGGCTCGGCGCCCGCGTTGAATGATTAA | 59.6 | 0.01 |
| mm4_exon_Dhx16_chr17_35880066_F | TCGTCGGCAGCGTCCAGTTCCTGATCGGCACCG | 60.8 | 0.00 |
| mm4_exon_Dhx16_chr17_35880066_R | GTCTCGTGGGCTCGGGCACACACCTTGCTCCAGA | 60.2 | 0.00 |
| mm3_intergenic_Gm26354|Gm7536_chr3_22701748_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Gm26354|Gm7536_chr3_22701748_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Gm24863|Gm24635_chr13_79973466_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Gm24863|Gm24635_chr13_79973466_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Gm10480|5730507C01Rik_chr12_18241085_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Gm10480|5730507C01Rik_chr12_18241085_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Onecut2_chr18_64346012_F | TCGTCGGCAGCGTCGATCTGGAACCTGATCCGGC | 60.2 | 0.00 |
| mm4_intron_Onecut2_chr18_64346012_R | GTCTCGTGGGCTCGGGTCAGACCTAAGAACTGGGCC | 60.0 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313995 |
AACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGA GGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAG |
| mm4_intergenic_Gm12947|4930471C06Rik_chr4_127433590 |
GTTGGTTGCTGTTGTGGACGCCCAAGAGCTGGCCTTCCAGCTTCCAGGGAGTCTCTGGTCTCCCAGTCCCATCTGCCCAT AC |
| mm4_exon_Slc46a2_chr4_59914869 |
CCACCACAGGCTCAATCCAAGTCCTCACCCGGAAGCCAGAGAGCCGGCTGCTCCACGGACAGGTGCCCCCTGGACCCATG TGACCTGCGTGACAGGGAACAGGGCTTG |
| mm4_exon_Gja10_chr4_32601087 |
CAGAGACCGTGAGCTTCCTGAGTCACTATGCCGTTGTCCCTTTTCTGACATCAGCCTCGATAGAAAGCTGGCCTTGCGAC TTCCTGGGCCTAATCTGTCTGTCCAAACCCCACTCTCTCTTGCACTG |
| mm4_intergenic_Ehd1|Cdc42bpg_chr19_6306393 |
CCCGCCCAGGAGTTACTTTTCGTGGCCCGCCCTCCCACTTCCTGGACGAGGGTCGGCCCCGCCCCCGCGCGCCTCCGCCT TCCCACCTTCACCTTCAGC |
| mm3_intergenic_Gm26993|Tle4_chr19_14431277 |
GCAGCCTACAAGCCACAAGATTGCTTATCTGTGAAGCTTGGGATACAAGGATTCAACACTCAGCCTGATGCCTGGAGGTG AGAGGGCCGGCTCCTGATTTCAGTTTAAAATTCCTGATTCTTATGTGGCTCCA |
| mm4_intergenic_Gm14254|Tox2_chr2_163166852 |
CCAGCCTTGCAACTCTGAGAATTTAGGGTATAGAGGTGAAACTGGAGCGCCCCCTCCAACTTCCTGGACGTTTGTCTCTT ACTCTGGCCCTTGGGT |
| mm4_intron_Rrm1_chr7_102461706 |
GGACTGGAAGCCTCTCAAGGAGAAGATTGCAAAGTAAGGAGTGAGGTGTAAAGGACCCTGGAGGCCGGAGGCCGGGCTCT TCTGGCTCTTCAGGAGTTGGATATGCTAGTGTGACCTCTGAAACT |
| mm4_exon_Aaas_chr15_102350500 |
ATTCCCACTTTCCGCATCCCCACCGCAGCGTTCCCACCCGGACCACCTGGCCCCGAAAGTCGGGAGGCGGGCTCTCATAG CTGTTGCCGGTCACCAGCTCGTTATT |
| mm4_intergenic_Ctc1|Aurkb_chr11_69040216 |
GAAGAGACGAGGGGACGACCGAGCCGACACTCTTACTTCCAGGGAGGAGACGGGACTTGTGGCCGGCGCCTAGCAGCGCA TCTCTAGATTTTGTCCCGCAGCGCGCCTTCCCTCAAGGAGTGGTGTCAC |
| mm4_intergenic_Gm23795|Gm5342_chr7_68974983 |
GTCAGGATGGGTCGGTCAATAGTTGAGTTTAACCTTTACCCCCTGGTGTAAAGGTTAAAGGGCTGAAGAAATTCCGGGAA GTCTGAGGACCAGCTGGCATGCTGATCAGCAGCTCGCCCTCCACTCACCTTCTCAG |
| mm4_intergenic_Arap3|Pcdh1_chr18_38041342 |
TCCTATGGGACTGCCTTCCATGTATGACAGAGACGACCCTCACACTTCCAGGATCCTGACAGCTCTTTGGATGACATAGG GATGAGGGCAGGT |
| mm4_intron_Tcf25_chr8_123396516 |
GCTGCAGAGTAAGCACGTCTTCCTCACAGCCCGCTGCAGCACGAACATGTCCCCAGGAAGTCGCTGGGACGGCTGGGTCG TGTTCTGGTAGACTACCAGCATGAGAGCAGTTCGGA |
| mm4_intron_Hipk2_chr6_38726659 |
ACTTGAAGGTTTCTAACACTAGAGATTCTTTTCCTGTAAGTCGGAGGTAGGGCTCTCCACACTATGATTAAAGGACTGAG ACAAATTTGGAAATGCTGAGCCTGTTGGTTCCACTC |
| mm4_intergenic_Gm12443|Anp32b_chr4_46450739 |
CGAAGGCTCAGGCGAAATTGGGTCCGGGAGAGCCCGGAAGAAGGCGCGAGGCTGAGCCTGCCCGTCGACCTCCAGGAGAG CGCGGGTGCCGGTGGGGGTGGGGGCGGGGGTCCCGGCACCGGAAGGTCTTACAT |
| mm4_exon_Cdca7_chr2_72483890 |
ATTTGGGGATGCAGAGAGCCGGAGAGGAACTCGGTTCATGATTCTTCTTCAGGACGATGATGTGCCCAGAAGTCGGAGGC CTGGATCCATGACCCTTCCGCATATAATCCGAC |
| mm4_exon_Zfp711_chrX_112600835 |
GAACGCCAGGACCTTCCAGGTCCACGGCTGCCGCCCCCCGCGGCAGTTGAGAGCGCGGCCTCCGACTGCCTGGCGGCGGG GAAGGCGGTGGCGGTTGCAGCCCCGCGGAGGCGGTGACAGGTAAGGGGACTCCTGAGC |
| mm4_intergenic_Sorbs1|Aldh18a1_chr19_40495825 |
GCAGACAAGCAAACAAGGGAGAGCACAGCCCGCCCTCCTGCTTCCTGGACTCACTACTGTGAGGTCTGCCATCATAGCTA CAATTGTTCTCTGCTCTAGAAGTCCTTCCTCTCTACCCCTGC |
| mm4_exon_Pkd2_chr5_104459987 |
CGACGAGGTGGAAGGAGAAGAAGGAGGGATGGTGGTAGAGATGGATGTGGAGTGGCGCCCGGGCAGTCGGAGGTCGGCCT CCTCCTCGGCCGTGAGCTC |
| mm4_intergenic_Gm24051|Gm11988_chr11_8047070 |
ATCACTGGAATGTGCAGGCCCACGATGTGGCCCTCCCACTTCCAGGTTTATGTGCCCTCAATTTATAATCTGTTTCTATG TCTCAGACACAAGAAACACATTGCTTAGTCTCCTCTGTCTAAGGTGTGT |
| mm4_exon_Kctd15_chr7_34641992 |
CGATCCGTTCACCCAAGTCAGGGGTGACCCGTACCACCAGGCAGTCGCAGGCCCGGCTGCGACGTCTTTGCTCTTGGTCT TGCTGCCAGCGCTCCAGCTCACGCACCATGGGCTGCAGCTGGTAGTACCTTGC |
| mm4_exon_Gm12979_chr4_129491987 | TGGCCGTGTATGTGTATCCGTCGGATCGGGCCCACCGACTTCTCGGACCCAGCCAGTATCACTCTTGGGCTACCTCCA |
| mm4_intron_Ttbk1_chr17_46468874 |
CTGCATGGCAACTGTCTAAGCTCTCCTTTTACACCACATTTGTATCGATTTGTTAGTGAGGCTTGAAATAAAACACCGTT GACAGAGGCCGCCCTGCGACTCCCTGGAAATGGCTGGTCCCGTTTCGTTTCCCAGGGGTA |
| mm4_intron_Ppapdc3_chr2_32096314 |
GGCCATCTGTCTGTCACCCGGCCCGCCAGGCCTTCTGGGAGCCGGCCCAACCCCTTCCAGGATCATCAGGCTCTGTGAGA AACCAAGGAACTCGTGTAATGAGGCTAGGCAGAAAGGGTGCCCCAGAAATGGACAAGCCT |
| mm4_exon_Mapk8ip3_chr17_24902252 |
AAAAGGAAGAGCTACCCGCCTCGAGCACCAGCTTTGCAGGGCAGTCCACAAGCAGCAGTGCCTGCTGTTGGACAGCACCT GGCAGTGGGAGCCCCGGCTCACCAGGAATGCTGGAGATACACAGGACG |
| mm4_intron_Abcd3_chr3_121811080 |
GGTGACCCTTCTCTAGCCCTTTAAGAATCTTAAAGAACAAAGGAGACAGAGAAATTACAGATAAGGCTGATCACATGGAT AAAGCTCCGAAGCCGGGAAGGAGGATAGCCGGCTCACAGAACAGTGGAGAAACTGGCCT |
| mm4_exon_Kcnab3_chr11_69326607 |
CGATCGCGCTATTACCACCACACCGGTGTCTGCCCTCGGACTTCCCGGCTCCCTCCTCCCGCTCCCTCGGAGCCCCCTCC CCCGCCCTGGCCATCCGGCATGCAGGTGTCTATCGCGTGTACCGAGCAGAACCTT |
| mm4_exon_1810041L15Rik_chr15_84380507 |
AGGCAGACACTGGAACACACATGGTGAGCCGTGTGACTCACCCAAGGCTGCCGCTGTCCCAGCAAAGCTCCCTGGCAGAC GGTGTGCCGGCTCCACGCCCTTTACACACACAGGAAATGCTCAGGAGGTTGCACAGGTT |
| mm4_intron_Trnp1_chr4_133497229 |
CCGGGTATCTGTCTCCTCCAATGGGAGTCACAGACTCAGAGGAGAATCCAGTCATACAGCCCTGAGCCAGCCCTCCTCCA TCCTGGGACTTTCTTCTTCTTCTCCTTGCAAGGGATGGAGCTGGGCTGTGTAGAAG |
| mm4_intergenic_Igsf1|n-R5s6_chrX_49798002 |
CCTGTCGCTCAGAGCTTCATTTGGTCACTGTATCCTGGAAGAGAGAGGGCCAGCTCCCAAGACAGCTGAAGCTGTATGTA GAGCCACCTCTTTAGCTCCCCAATGCCTTTAAAGCGACCTCGGGT |
| mm4_intron_Cd63_chr10_128909668 |
CAGAGAGAACCAGACGGCGCCTGGGTTGCAGAGGGCTCCGGGAACCCTGAGGTCCGGCTCCTGGCTCGAGCCTTCCAGGC AGCGGCCACGTGGCCTTGTCCTGGCGGGGGGCTCACCTCCCCCTGCTAGCCTGGTGACTC |
| mm4_intergenic_Cox6c|Rgs22_chr15_35984810 |
GAGACACATTCCGCACCAGACCAGGTTCCCTCTCCTCTGAAAGGCCAGGAAGGAGGAGGGCAGCCTCACCATGCTTGCTG CAAGGAGGAGCAGGGGGAAGGGGAACTTCTGTGAGAAAACCTGGAGGCAAGCT |
| mm4_intergenic_Hoxd11|Hoxd10_chr2_74690095 |
ACAGTTTCCGGGTCCGTTTTACTTTCTTGATTGCCGGGAACGGGACCCGGAAGGCCGAAGGACGGCTCCCACACACAGGC AGGGCCTCTGGTCCAGGGCAAGTCTTTGGA |
| mm4_intergenic_Gm23772|9130230N09Rik_chr11_22858804 |
TAGGCGAGTGCATTTGAGCAGGTGCTAGGTGTTACGTCCCCAGAAGGGTCCCAGCCCCTCAGGTGCGCCCACCTGCCAGT CGCAGGGCCAGCTCCCCAGGCAGTCCTGAGTCTAGTTAATCATTCAACGCGGGCG |
| mm4_exon_Dhx16_chr17_35880066 |
CAGTTCCTGATCGGCACCGCACAGCGCTGCGCCTCCGCCGAGGAGTTCGTGCAGCGCCTGCGGGACACCGACACCCTGGA CCTCGGGGGGCCGGCGCGGGACTTCGCCCTGAAGCTCTGGAGCAAGGTGTGTGC |
| mm4_intron_Onecut2_chr18_64346012 |
GATCTGGAACCTGATCCGGCGCGTTTCCAGTAAATCCGACGGCCGGCTCGCTCTTCCCAGAAGAGCGCTCGCCAGTGCCC AGGATCCCTGGCGCTCGGGCCACGCCAGCTGAGGCCCAGTTCTTAGGTCTGAC |