CRISPOR

DNA break repair outcome predictions

Guide: GACATGGTTATGTTTCTGGA TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGGGGATGGAGAGCCGAAAGGACATGGTTATGTTTCTGGATGGGGGTCAGCTTGGCACTCTGGTTGGTAAGAGGGTCTCT`	(Wild-type sequence)
409	`GAGCCGAAAGGACATGGTTATGTTTCTGG----GGGTCAGCTTGGCACTCTGGTTGGTAA`	`4 bp deleted → frameshift`
311	`GAGCCGAAAGGACATGGTTATGTTTCTGG-----GGTCAGCTTGGCACTCTGGTTGGTAA`	`5 bp deleted → frameshift`
296	`GAGCCGAAAGGACATGGTTATGTTTCTGG------GTCAGCTTGGCACTCTGGTTGGTAA`	`6 bp deleted → no frameshift`
282	`GAGCCGAAAGGACATGGTTATGTTTCTGG-------TCAGCTTGGCACTCTGGTTGGTAA`	`7 bp deleted → frameshift`
240	`GAGCCGAAAGGACATGGTTATGTTTCTGG------------------------TTGGTAA`	`24 bp deleted → no frameshift`
224	`GAGCCGAAAGGACATGGTTATGTTTCTGG----------------CACTCTGGTTGGTAA`	`16 bp deleted → frameshift`
224	`GAGCCGAAAGGACATGG----------------GGGTCAGCTTGGCACTCTGGTTGGTAA`	`16 bp deleted → frameshift`
193	`GAGCCGAAAGGACATGGT-------------------CAGCTTGGCACTCTGGTTGGTAA`	`19 bp deleted → frameshift`
182	`GAGCCGAAAGGACATGGTTATG----------GGGGTCAGCTTGGCACTCTGGTTGGTAA`	`10 bp deleted → frameshift`
170	`GAGCCGAAAGGACATGG-----------------GGTCAGCTTGGCACTCTGGTTGGTAA`	`17 bp deleted → frameshift`
164	`GAGCCGAAAGGACATGGTTATGTTTC------------AGCTTGGCACTCTGGTTGGTAA`	`12 bp deleted → no frameshift`
162	`GAGCCGAAAGGACATGG------------------GTCAGCTTGGCACTCTGGTTGGTAA`	`18 bp deleted → no frameshift`
149	`GAGCCGAAAGGACATGGTTATGT--------------CAGCTTGGCACTCTGGTTGGTAA`	`14 bp deleted → frameshift`
141	`GAGCCGAAAGGACATGGTTATGTTTCT---------------TGGCACTCTGGTTGGTAA`	`15 bp deleted → no frameshift`
133	`GAGCCGAAAGG----------------------GGGTCAGCTTGGCACTCTGGTTGGTAA`	`22 bp deleted → frameshift`
126	`GAGCCGAAAGG-----------------------GGTCAGCTTGGCACTCTGGTTGGTAA`	`23 bp deleted → frameshift`
123	`GAGCCGAAAGGACATGGTTATGTTTCTGG----------------------------TAA`	`28 bp deleted → frameshift`
123	`GAGCCGAAAGGACATGG----------------------------CACTCTGGTTGGTAA`	`28 bp deleted → frameshift`
120	`GAGCCGAAAGG------------------------GTCAGCTTGGCACTCTGGTTGGTAA`	`24 bp deleted → no frameshift`
115	`GAGCCGAAAGGACATGGTT------------------------------------GGTAA`	`36 bp deleted → no frameshift`
114	`GAGCCGAAAGG-------------------------TCAGCTTGGCACTCTGGTTGGTAA`	`25 bp deleted → frameshift`
99	`GAGCCGAAAGGACATGGTTATGTTTCT----------------------CTGGTTGGTAA`	`22 bp deleted → frameshift`
99	`GAGCCGAAAGGACATGGTTATG----------------------GCACTCTGGTTGGTAA`	`22 bp deleted → frameshift`
86	`GAGCCGAAAGGACA-------------------------GCTTGGCACTCTGGTTGGTAA`	`25 bp deleted → frameshift`
84	`GAGCCGAAAGGACATGGTTATGTT-------------------------------GGTAA`	`31 bp deleted → frameshift`
81	`GAGCCGAAAGGACATGGTTATGTTT------------------GGCACTCTGGTTGGTAA`	`18 bp deleted → no frameshift`
81	`GAGCCGAAAGGACATGGT----------------------------------------AA`	`40 bp deleted → frameshift`
78	`GAGC-------------------------------------TTGGCACTCTGGTTGGTAA`	`37 bp deleted → frameshift`
77	`GAGCCGAAAGGACATGGTTATGTT-------------------GGCACTCTGGTTGGTAA`	`19 bp deleted → frameshift`
73	`GAGCCGAAAGG----------------------------------CACTCTGGTTGGTAA`	`34 bp deleted → frameshift`
66	`GAGCCGAAAGGACATGGTTATG------------------------------GTTGGTAA`	`30 bp deleted → no frameshift`
66	`GAGCCGAAAG------------------------------CTTGGCACTCTGGTTGGTAA`	`30 bp deleted → no frameshift`
60	`GAGCCGAAAGGACATGGTT------------------------GGCACTCTGGTTGGTAA`	`24 bp deleted → no frameshift`
57	`GAGCCGAAAGGACA---------------------------------CTCTGGTTGGTAA`	`33 bp deleted → no frameshift`
54	`GAGCCGAAAGGACATGGTTATG----------------------------------GTAA`	`34 bp deleted → frameshift`
52	`GAGCCGAAAGGACATGGTTATGT-----------------------------------AA`	`35 bp deleted → frameshift`
52	`GAGCCGAAAGGAC-----------------------------------TCTGGTTGGTAA`	`35 bp deleted → frameshift`
48	`GAGCCGAAAGG------------------------------------------TTGGTAA`	`42 bp deleted → no frameshift`
48	`GAGC------------------------------------------ACTCTGGTTGGTAA`	`42 bp deleted → no frameshift`
44	`GAGCCGAAAGGACATGGTTATGTTT------------------------------GGTAA`	`30 bp deleted → no frameshift`
40	`GAGCCGAAAGG----------------------------------------------TAA`	`46 bp deleted → frameshift`
28	`GAGCCGAAAGGACATGGTTA---------------------------------------A`	`39 bp deleted → no frameshift`
15	`GAGCCGAAA---------------------------------------------------`	`51 bp deleted → no frameshift`
14	`GAGCCGAA----------------------------------------------------`	`52 bp deleted → frameshift`

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