CRISPOR

DNA break repair outcome predictions

Guide: TGGAGCGGCCATGCTTGGCC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACCCCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCA`	(Wild-type sequence)
311	`CACGGTCTGCTGGAGCGGCCATGCTTGG-----GACCCCCAGTCCCCTCCGCGGACAGCC`	`5 bp deleted → frameshift`
296	`CACGGTCTGCTGGAGCGGCCATGCTTGG------ACCCCCAGTCCCCTCCGCGGACAGCC`	`6 bp deleted → no frameshift`
283	`CACGGTCTGCTGGAGCGG---------------GACCCCCAGTCCCCTCCGCGGACAGCC`	`15 bp deleted → no frameshift`
282	`CACGGTCTGCTGGAGCGGCCATGCTTGGCC-------CCCAGTCCCCTCCGCGGACAGCC`	`7 bp deleted → frameshift`
268	`CACGGTCTGCTGGAGCGGCCATGCTTGGCC--------CCAGTCCCCTCCGCGGACAGCC`	`8 bp deleted → frameshift`
255	`CACGGTCTGCTGGAGCGGCCATGCTTGGCC---------CAGTCCCCTCCGCGGACAGCC`	`9 bp deleted → no frameshift`
242	`CACGGTCTGCTGGAGCGGCCATGCTTGGCC----------AGTCCCCTCCGCGGACAGCC`	`10 bp deleted → frameshift`
188	`CACGGTCTGCTGGAGCGGCCATGCTTGGCC---------------CCTCCGCGGACAGCC`	`15 bp deleted → no frameshift`
184	`CACGGTCTGCTGGAGCGGCCA--------------------GTCCCCTCCGCGGACAGCC`	`20 bp deleted → frameshift`
179	`CACGGTCTGCTGGAGCGGCCATGCTTGGCC----------------CTCCGCGGACAGCC`	`16 bp deleted → frameshift`
179	`CACGGTCTGCTGGAGCGG----------------ACCCCCAGTCCCCTCCGCGGACAGCC`	`16 bp deleted → frameshift`
175	`CACGGTCTGCTGGA---------------------CCCCCAGTCCCCTCCGCGGACAGCC`	`21 bp deleted → no frameshift`
170	`CACGGTCTGCTGGAGCGGCCATGCTTGGCC-----------------TCCGCGGACAGCC`	`17 bp deleted → frameshift`
170	`CACGGTCTGCTGGAGCGGCC-----------------CCCAGTCCCCTCCGCGGACAGCC`	`17 bp deleted → frameshift`
162	`CACGGTCTGCTGGAGCGGCC------------------CCAGTCCCCTCCGCGGACAGCC`	`18 bp deleted → no frameshift`
154	`CACGGTCTGCTGGAGCGGCC-------------------CAGTCCCCTCCGCGGACAGCC`	`19 bp deleted → frameshift`
148	`CACGG----------------------------GACCCCCAGTCCCCTCCGCGGACAGCC`	`28 bp deleted → frameshift`
147	`CACGGTCTGCTGGAGCGGCCATGCTTGGCC--------------------GCGGACAGCC`	`20 bp deleted → frameshift`
147	`CACGGTCTGCTGG--------------------GACCCCCAGTCCCCTCCGCGGACAGCC`	`20 bp deleted → frameshift`
133	`CACGGTCTGCTGGAGCGGCCATGCTTGGCC------------------------------`	`30 bp deleted → no frameshift`
132	`CACGGTCTGCTGGAGCGG------------------------------------ACAGCC`	`36 bp deleted → no frameshift`
126	`CACGGTCTGCTGGAGCGGCCATGCTTGGC-----------------------GGACAGCC`	`23 bp deleted → frameshift`
114	`CACGGTCTGCTGGAGCGGCC-------------------------CCTCCGCGGACAGCC`	`25 bp deleted → frameshift`
109	`CACGGTCTGCTGGAGCGGCCATGCTTGG--------------------------ACAGCC`	`26 bp deleted → frameshift`
109	`CACGGTCTGCTGGAGCGGCC--------------------------CTCCGCGGACAGCC`	`26 bp deleted → frameshift`
103	`CACGGTCTGCTGGAGCGGCC---------------------------TCCGCGGACAGCC`	`27 bp deleted → no frameshift`
98	`CACGGTCTGCTGGAGCGGCCATGC----------------------------GGACAGCC`	`28 bp deleted → frameshift`
95	`CACGGTCTGCTGGAGCGGCCATGCT-----------------------CCGCGGACAGCC`	`23 bp deleted → frameshift`
94	`CACGG-----------------------------ACCCCCAGTCCCCTCCGCGGACAGCC`	`29 bp deleted → frameshift`
89	`CACGGTCTGCTGGAGCGGCC------------------------------GCGGACAGCC`	`30 bp deleted → no frameshift`
81	`CACGGTCTGCTGGAGCGGCC----------------------------------------`	`40 bp deleted → frameshift`
78	`CACGGTC-------------------------------------CCCTCCGCGGACAGCC`	`37 bp deleted → frameshift`
77	`CACGGTCTGCTGGAG---------------------------TCCCCTCCGCGGACAGCC`	`27 bp deleted → no frameshift`
76	`CACGGTCTGCTGGAGCGGC---------------------------------GGACAGCC`	`33 bp deleted → no frameshift`
73	`CACGGTCTGCTGGAGCG----------------------------------CGGACAGCC`	`34 bp deleted → frameshift`
69	`CACGGTCTGCTGGAGCGGCCATGC-----------------------------------C`	`35 bp deleted → frameshift`
64	`CACGGTCTGCTGGA-----------------------------------------CAGCC`	`41 bp deleted → frameshift`
58	`CACGGTCTGCTGGAGC-------------------------------------------C`	`43 bp deleted → frameshift`
57	`CAC---------------------------------CCCCAGTCCCCTCCGCGGACAGCC`	`33 bp deleted → no frameshift`
51	`CACGG-------------------------------------------------ACAGCC`	`49 bp deleted → frameshift`
49	`CACGGTCTGCTGGAGCGGCCA------------------------------------GCC`	`36 bp deleted → no frameshift`
48	`CACGGTCTGC------------------------------------------GGACAGCC`	`42 bp deleted → no frameshift`
47	`CACGGTCTGCT-------------------------------------CCGCGGACAGCC`	`37 bp deleted → frameshift`
42	`CA---------------------------------------GTCCCCTCCGCGGACAGCC`	`39 bp deleted → no frameshift`
40	`CACGGTCT----------------------------------------CCGCGGACAGCC`	`40 bp deleted → frameshift`
38	`CACG-----------------------------------------------CGGACAGCC`	`47 bp deleted → frameshift`
36	`CACGGTC------------------------------------------CGCGGACAGCC`	`42 bp deleted → no frameshift`
34	`CACGGTCTGC-------------------------------------------------C`	`49 bp deleted → frameshift`
21	`CAC-----------------------------------------------------AGCC`	`53 bp deleted → frameshift`
19	`CA-------------------------------------------------------GCC`	`55 bp deleted → frameshift`

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