CRISPOR

DNA break repair outcome predictions

Guide: GGCGACCGCGGAGATGGCAG TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGCCCCAGCGCCGTGTCGACGGCGACCGCGGAGATGGCAGTGGGGAGCTAGGGTCACCTTGTGATGCGAGCGCTCCTCTG`	(Wild-type sequence)
370	`CCGTGTCGACGGCGACCGCGGAGATGG------GGAGCTAGGGTCACCTTGTGATGCGAG`	`6 bp deleted → no frameshift`
347	`CCGTGTCGACGGCGACCGCGGAG--------------CTAGGGTCACCTTGTGATGCGAG`	`14 bp deleted → frameshift`
282	`CCGTGTCGACGGCGACCGCGGAGATGG-------GAGCTAGGGTCACCTTGTGATGCGAG`	`7 bp deleted → frameshift`
268	`CCGTGTCGACGGCGACCGCGGAGATGG--------AGCTAGGGTCACCTTGTGATGCGAG`	`8 bp deleted → frameshift`
242	`CCGTGTCGACGGCGACCGCGGAGATGGC----------TAGGGTCACCTTGTGATGCGAG`	`10 bp deleted → frameshift`
219	`CCGTGTCGACGGCGACCGCGG------------GGAGCTAGGGTCACCTTGTGATGCGAG`	`12 bp deleted → no frameshift`
211	`CCGTGTCGACGGCGACCGCGGAGATGGCAG-------CTAGGGTCACCTTGTGATGCGAG`	`7 bp deleted → frameshift`
208	`CCGTGTCGACGGCGACCGCGG-------------GAGCTAGGGTCACCTTGTGATGCGAG`	`13 bp deleted → frameshift`
188	`CCGTGTCGACGGCGACCGCGGAGATGG---------------GTCACCTTGTGATGCGAG`	`15 bp deleted → no frameshift`
179	`CCGTGTCGACGGCGACCGCGGAGATGG----------------TCACCTTGTGATGCGAG`	`16 bp deleted → frameshift`
173	`CCGTGTCGACGGCGACCGCGGAGATGGCAG-----------GGTCACCTTGTGATGCGAG`	`11 bp deleted → frameshift`
164	`CCGTGTCGACGGCGACCGCGGAGA------------GCTAGGGTCACCTTGTGATGCGAG`	`12 bp deleted → no frameshift`
154	`CCGTGTCGACGGCGACCGC-------------------TAGGGTCACCTTGTGATGCGAG`	`19 bp deleted → frameshift`
140	`CCGTGTCGACGGCGACCGCGG---------------------GTCACCTTGTGATGCGAG`	`21 bp deleted → no frameshift`
140	`CCGTGTCGACGG---------------------GGAGCTAGGGTCACCTTGTGATGCGAG`	`21 bp deleted → no frameshift`
133	`CCGTGTCGACGGCGACCGCGGAGATG------------------------------CGAG`	`30 bp deleted → no frameshift`
133	`CCGTGTCGACGGCGACCGCGG----------------------TCACCTTGTGATGCGAG`	`22 bp deleted → frameshift`
133	`CCGTGTCGACGG----------------------GAGCTAGGGTCACCTTGTGATGCGAG`	`22 bp deleted → frameshift`
128	`CCGTGTCGACGGCGACCGCGGAGATGGCA-----------------CCTTGTGATGCGAG`	`17 bp deleted → frameshift`
126	`CCGTGTCGACGG-----------------------AGCTAGGGTCACCTTGTGATGCGAG`	`23 bp deleted → frameshift`
122	`CCGTGTCGACGGCGACCGCGGAG------------------GGTCACCTTGTGATGCGAG`	`18 bp deleted → no frameshift`
114	`CCGTGTCGACGGC-------------------------TAGGGTCACCTTGTGATGCGAG`	`25 bp deleted → frameshift`
106	`CCGTGTCGACGGCGACC-------------------------------TTGTGATGCGAG`	`31 bp deleted → frameshift`
105	`CCGTGTCGACGGCGA---------------------GCTAGGGTCACCTTGTGATGCGAG`	`21 bp deleted → no frameshift`
94	`CCGTGTCGACGGCGACCGCGGAGATGGC-----------------------------GAG`	`29 bp deleted → frameshift`
90	`CCGTGTCGACGGCGACCGCG--------------------------------------AG`	`38 bp deleted → frameshift`
89	`CCGTGTCGACGG------------------------------GTCACCTTGTGATGCGAG`	`30 bp deleted → no frameshift`
86	`CCGTGTCGACGGCGACCGCGGAGATG-------------------------TGATGCGAG`	`25 bp deleted → frameshift`
84	`CCGTGTCGACGG-------------------------------TCACCTTGTGATGCGAG`	`31 bp deleted → frameshift`
78	`CCGTGTCGACGGCGACCGCGGAG-------------------------------------`	`37 bp deleted → frameshift`
77	`CCGTGTCGACGGCGACCGCGGAGATG---------------------------ATGCGAG`	`27 bp deleted → no frameshift`
77	`CCGTGTCGACGGCGA--------------------------------------------G`	`44 bp deleted → frameshift`
77	`CCGTGTCGA---------------------------GCTAGGGTCACCTTGTGATGCGAG`	`27 bp deleted → no frameshift`
77	`CCGTG---------------------------GGGAGCTAGGGTCACCTTGTGATGCGAG`	`27 bp deleted → no frameshift`
75	`CCGTGTC--------------------------------------ACCTTGTGATGCGAG`	`38 bp deleted → frameshift`
66	`CCGTGTCGACGGCGACCGCGGAGATGGCAG------------------------------`	`30 bp deleted → no frameshift`
60	`CCGTGTCGACGGCGACCGCGGA--------------------------------TGCGAG`	`32 bp deleted → frameshift`
54	`CCGTGTCGACGGCGACCG----------------------------------------AG`	`40 bp deleted → frameshift`
52	`CCGTGTCGACGGCGACCGCGGAGA-----------------------------------G`	`35 bp deleted → frameshift`
47	`CCGTGTCGAC-------------------------------------CTTGTGATGCGAG`	`37 bp deleted → frameshift`
45	`CCGTG------------------------------------------------ATGCGAG`	`48 bp deleted → no frameshift`
42	`CCGTGTCGACGGCGA---------------------------------------TGCGAG`	`39 bp deleted → no frameshift`
41	`CCGTGTCGA--------------------------------------------------G`	`50 bp deleted → frameshift`
40	`CCGT----------------------------------------CACCTTGTGATGCGAG`	`40 bp deleted → frameshift`
40	`CCGTGT----------------------------------------------GATGCGAG`	`46 bp deleted → frameshift`
40	`CC----------------------------------------------TTGTGATGCGAG`	`46 bp deleted → frameshift`
38	`CCGTGTCGACG-----------------------------------------------AG`	`47 bp deleted → frameshift`
31	`CCGTGTCGA---------------------------------------------TGCGAG`	`45 bp deleted → no frameshift`
25	`CCG-------------------------------------------------------AG`	`55 bp deleted → frameshift`
23	`CCGTG---------------------------------------------------CGAG`	`51 bp deleted → no frameshift`

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