CRISPOR

DNA break repair outcome predictions

Guide: CGCTCCAGCAGACCGTGGCT CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGTCCCGGGCCAAGCATGGCCGCTCCAGCAGACCGTGGCTCGGCGCCCAGCCCTGCAGCACTGCCTGCTAGTCCATCGAC`	(Wild-type sequence)
467	`CAAGCATGGCCGCTCCAGCAGACCGTGGC-----GCCCAGCCCTGCAGCACTGCCTGCTA`	`5 bp deleted → frameshift`
299	`CAAGCATGGCCGCTCCAGC----------------------CCTGCAGCACTGCCTGCTA`	`22 bp deleted → frameshift`
282	`CAAGCATGGCCGCTCCAGCAGACCGTGGC-------CCAGCCCTGCAGCACTGCCTGCTA`	`7 bp deleted → frameshift`
282	`CAAGCATGGCCGCTCCAGCAGACCG-------GCGCCCAGCCCTGCAGCACTGCCTGCTA`	`7 bp deleted → frameshift`
242	`CAAGCATGGCCGCTCCAGCAGACCG----------CCCAGCCCTGCAGCACTGCCTGCTA`	`10 bp deleted → frameshift`
219	`CAAGCATGGCCGCTCCAGCAGACCGTGGC------------CCTGCAGCACTGCCTGCTA`	`12 bp deleted → no frameshift`
208	`CAAGCATGGCCGCTCCAGCAGACC-------------CAGCCCTGCAGCACTGCCTGCTA`	`13 bp deleted → frameshift`
198	`CAAGCATGGCCGCTCCAGCAGACC--------------AGCCCTGCAGCACTGCCTGCTA`	`14 bp deleted → frameshift`
193	`CAAGCATGGCCGCTCCAGCAG-------------------CCCTGCAGCACTGCCTGCTA`	`19 bp deleted → frameshift`
190	`CAAGCATGGCCGC-----------------------CCAGCCCTGCAGCACTGCCTGCTA`	`23 bp deleted → frameshift`
188	`CAAGCATGGCCGCTCCAGC---------------GCCCAGCCCTGCAGCACTGCCTGCTA`	`15 bp deleted → no frameshift`
181	`CAAGCATGGCCGCTCCAGCAG---------------------------CACTGCCTGCTA`	`27 bp deleted → no frameshift`
180	`CAAGCATGGC------------------------GCCCAGCCCTGCAGCACTGCCTGCTA`	`24 bp deleted → no frameshift`
178	`CAAGCATGGCCGCTCCAGCA------------------------------CTGCCTGCTA`	`30 bp deleted → no frameshift`
170	`CAAGCATGGCCGCTCCAGCAGACCGTGGC-----------------AGCACTGCCTGCTA`	`17 bp deleted → frameshift`
170	`CAAGCATGGCCGCTCCAGC-----------------CCAGCCCTGCAGCACTGCCTGCTA`	`17 bp deleted → frameshift`
163	`CAAGCATGGCC--------------------------CAGCCCTGCAGCACTGCCTGCTA`	`26 bp deleted → frameshift`
162	`CAAGCATGGCCGCTCCAGCAGACC------------------CTGCAGCACTGCCTGCTA`	`18 bp deleted → no frameshift`
154	`CAAGCATGGCCGCTCCAGCAGACC-------------------TGCAGCACTGCCTGCTA`	`19 bp deleted → frameshift`
149	`CAAGCATGGCCGCTCCAGCAGACCGTGGCT--------------GCAGCACTGCCTGCTA`	`14 bp deleted → frameshift`
147	`CAAGCATGGCCGCTCCAGCAGACCGTGGC--------------------ACTGCCTGCTA`	`20 bp deleted → frameshift`
147	`CAAGCATGGCCG--------------------GCGCCCAGCCCTGCAGCACTGCCTGCTA`	`20 bp deleted → frameshift`
140	`CAAGCATGGCCGCTCC---------------------CAGCCCTGCAGCACTGCCTGCTA`	`21 bp deleted → no frameshift`
140	`CAAGCATGGCCGC---------------------GCCCAGCCCTGCAGCACTGCCTGCTA`	`21 bp deleted → no frameshift`
127	`CAAGCATGGCC-------------------------------CTGCAGCACTGCCTGCTA`	`31 bp deleted → frameshift`
122	`CAAGCATGGCCGCTCCAGCAGACCGTG------------------CAGCACTGCCTGCTA`	`18 bp deleted → no frameshift`
117	`CAAGCATGGCCGCTCCAGCAGACCGTGGCT-----------------------------A`	`29 bp deleted → frameshift`
114	`CAAGCATGGCCGCTCCAGCAGACCGTGGC-------------------------CTGCTA`	`25 bp deleted → frameshift`
109	`CAAGCATGGCCGCTCC--------------------------CTGCAGCACTGCCTGCTA`	`26 bp deleted → frameshift`
103	`CAAGCATGGCCGCTCC---------------------------TGCAGCACTGCCTGCTA`	`27 bp deleted → no frameshift`
103	`CAAGCATGGCC---------------------------AGCCCTGCAGCACTGCCTGCTA`	`27 bp deleted → no frameshift`
99	`CAAGCATGGCCGCTCCAGCAGACCGTGGCT----------------------GCCTGCTA`	`22 bp deleted → frameshift`
98	`CAAGCATGGCCGC----------------------------CCTGCAGCACTGCCTGCTA`	`28 bp deleted → frameshift`
94	`CAAGC-----------------------------GCCCAGCCCTGCAGCACTGCCTGCTA`	`29 bp deleted → frameshift`
84	`CAAGCATGGCCGCTCCAGCAGACC-------------------------------TGCTA`	`31 bp deleted → frameshift`
84	`CAAGC-------------------------------CCAGCCCTGCAGCACTGCCTGCTA`	`31 bp deleted → frameshift`
82	`CAAGC------------------------------------CCTGCAGCACTGCCTGCTA`	`36 bp deleted → no frameshift`
81	`CAAGCATGGCCGCTCCAGCAGACCGTGGCT--------------------------GCTA`	`26 bp deleted → frameshift`
81	`CAAGCATGGCCGCTCCAGCAGACCGTG--------------------------CCTGCTA`	`26 bp deleted → frameshift`
80	`CAAGCATGGCC--------------------------------TGCAGCACTGCCTGCTA`	`32 bp deleted → frameshift`
76	`CAAGCATGGCCGC---------------------------------AGCACTGCCTGCTA`	`33 bp deleted → no frameshift`
74	`CAAGCATGGCCGCTCCAGCAGAC----------------------------TGCCTGCTA`	`28 bp deleted → frameshift`
69	`CAAGCATGGCCGCTCCAGC-----------------------------------CTGCTA`	`35 bp deleted → frameshift`
66	`CAAGCATGGCCGCTCCAGCAGACCGTG------------------------------CTA`	`30 bp deleted → no frameshift`
66	`CAAGCATGGCCGCT------------------------------GCAGCACTGCCTGCTA`	`30 bp deleted → no frameshift`
66	`CAAGCATGGCC--------------------------------------------TGCTA`	`44 bp deleted → frameshift`
66	`CAAGCA--------------------------------------------CTGCCTGCTA`	`44 bp deleted → frameshift`
66	`CAAGCATGGCCGC------------------------------------ACTGCCTGCTA`	`36 bp deleted → no frameshift`
66	`CAAGCATGGC------------------------------------AGCACTGCCTGCTA`	`36 bp deleted → no frameshift`
64	`CAAGCA-----------------------------------------GCACTGCCTGCTA`	`41 bp deleted → frameshift`
57	`CAAGCATGGCCGCTCCA---------------------------------CTGCCTGCTA`	`33 bp deleted → no frameshift`
57	`CAAGCA---------------------------------GCCCTGCAGCACTGCCTGCTA`	`33 bp deleted → no frameshift`
56	`CAAGCATGGCCGCTCCAGC---------------------------------------TA`	`39 bp deleted → no frameshift`
56	`CAAGCATGGCCGCTCC---------------------------------------TGCTA`	`39 bp deleted → no frameshift`
56	`CAAGCATGGC---------------------------------------ACTGCCTGCTA`	`39 bp deleted → no frameshift`
52	`CAAGCATGGCCGCT---------------------------------------------A`	`45 bp deleted → no frameshift`
51	`CAAGCATGGCCGC-----------------------------------------CTGCTA`	`41 bp deleted → frameshift`
47	`CAAGCATG-------------------------------------CAGCACTGCCTGCTA`	`37 bp deleted → frameshift`
47	`CA-------------------------------------GCCCTGCAGCACTGCCTGCTA`	`37 bp deleted → frameshift`
45	`CAAGCATGGCCGCT--------------------------------------GCCTGCTA`	`38 bp deleted → frameshift`
36	`CAAGCATGGCCGCT------------------------------------------GCTA`	`42 bp deleted → no frameshift`
36	`CAAGCATGGC------------------------------------------------TA`	`48 bp deleted → no frameshift`
34	`CAAGC-------------------------------------------------CTGCTA`	`49 bp deleted → frameshift`
31	`CAAGCATG---------------------------------------------CCTGCTA`	`45 bp deleted → no frameshift`
31	`CA---------------------------------------------GCACTGCCTGCTA`	`45 bp deleted → no frameshift`
28	`CAAGC-----------------------------------------------------TA`	`53 bp deleted → frameshift`
27	`CA------------------------------------------------CTGCCTGCTA`	`48 bp deleted → no frameshift`
25	`CAAGCATG-------------------------------------------------CTA`	`49 bp deleted → frameshift`

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