CRISPOR

DNA break repair outcome predictions

Guide: CAGGGCTGGGCGCCGAGCCA CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACCCCCAG`	(Wild-type sequence)
314	`GGCAGTGCTGCAGGGCTGGGCGCCGAGC----------------GGCCATGCTTGGCCCG`	`16 bp deleted → frameshift`
299	`GGCAGTGCTGCAGGGCTGG----------------------AGCGGCCATGCTTGGCCCG`	`22 bp deleted → frameshift`
282	`GGCAGTGCTGCAGGGCTGGGCGCCG-------GTCTGCTGGAGCGGCCATGCTTGGCCCG`	`7 bp deleted → frameshift`
270	`GGCAGTGCTGCAGGGCTGGGCGCCGAGCCA-------------------TGCTTGGCCCG`	`19 bp deleted → frameshift`
242	`GGCAGTGCTGCAGGGCTGGGCGCCGAGC----------TGGAGCGGCCATGCTTGGCCCG`	`10 bp deleted → frameshift`
242	`GGCAGTGCTGCAGGGCTGGGCG----------GTCTGCTGGAGCGGCCATGCTTGGCCCG`	`10 bp deleted → frameshift`
208	`GGCAGTGCTGCAGGGCTGGG-------------TCTGCTGGAGCGGCCATGCTTGGCCCG`	`13 bp deleted → frameshift`
198	`GGCAGTGCTGCAGGGCTGG--------------TCTGCTGGAGCGGCCATGCTTGGCCCG`	`14 bp deleted → frameshift`
193	`GGCAGTGCTGCAGGGCTG-------------------CTGGAGCGGCCATGCTTGGCCCG`	`19 bp deleted → frameshift`
190	`GGCAGTGCTGCAGGGCTGGGCG-----------------------GCCATGCTTGGCCCG`	`23 bp deleted → frameshift`
188	`GGCAGTGCTGCAGGGCTGGGCGC---------------TGGAGCGGCCATGCTTGGCCCG`	`15 bp deleted → no frameshift`
181	`GGCAGTGCTGC---------------------------TGGAGCGGCCATGCTTGGCCCG`	`27 bp deleted → no frameshift`
180	`GGCAGTGCTGCAGGGCTGGGCGCC------------------------ATGCTTGGCCCG`	`24 bp deleted → no frameshift`
178	`GGCAGTGCTG------------------------------GAGCGGCCATGCTTGGCCCG`	`30 bp deleted → no frameshift`
170	`GGCAGTGCTGCAGGGCTGGGC-----------------TGGAGCGGCCATGCTTGGCCCG`	`17 bp deleted → frameshift`
163	`GGCAGTGCTGCAGGGCTGGGC--------------------------CATGCTTGGCCCG`	`26 bp deleted → frameshift`
162	`GGCAGTGCTGCAGGG------------------TCTGCTGGAGCGGCCATGCTTGGCCCG`	`18 bp deleted → no frameshift`
154	`GGCAGTGCTGCAGG-------------------TCTGCTGGAGCGGCCATGCTTGGCCCG`	`19 bp deleted → frameshift`
147	`GGCAGTGCTGCAGGGCTGGGCGCCG--------------------GCCATGCTTGGCCCG`	`20 bp deleted → frameshift`
141	`GGCAGTGCTGCAGGGCTGGGCGCCGAGCC-----------------------------CG`	`29 bp deleted → frameshift`
140	`GGCAGTGCTGCAGGGCTGGGCGC---------------------GGCCATGCTTGGCCCG`	`21 bp deleted → no frameshift`
140	`GGCAGTGCTGCAGGGCTGGG---------------------AGCGGCCATGCTTGGCCCG`	`21 bp deleted → no frameshift`
127	`GGCAGTGCTGCAGGGC-------------------------------CATGCTTGGCCCG`	`31 bp deleted → frameshift`
120	`GGCAGTGCTGCAGGGCTGGGCGCCGAGC------------------------TTGGCCCG`	`24 bp deleted → no frameshift`
109	`GGCAGTGCTGCAGGGCTGGGCGCC----------------------------------CG`	`34 bp deleted → frameshift`
109	`GGCAGTGCTGCAGGG--------------------------AGCGGCCATGCTTGGCCCG`	`26 bp deleted → frameshift`
104	`GGCAGTGCTGCAGGGCTGGGCGCCG-----------------------------------`	`35 bp deleted → frameshift`
103	`GGCAGTGCTGCAGGGCTGG---------------------------CCATGCTTGGCCCG`	`27 bp deleted → no frameshift`
103	`GGCAGTGCTGCAGG---------------------------AGCGGCCATGCTTGGCCCG`	`27 bp deleted → no frameshift`
99	`GGCAGTGCTGCAGGGCTGGGC------------------------------------CCG`	`36 bp deleted → no frameshift`
98	`GGCAGTGCTGCAGGGC----------------------------GGCCATGCTTGGCCCG`	`28 bp deleted → frameshift`
94	`GGCAGTGCTGCAGGGCTGGGCGC-----------------------------TTGGCCCG`	`29 bp deleted → frameshift`
89	`GGCAGTGCTGCAGGGCTGGGCGCCGAGCC------------------------------G`	`30 bp deleted → no frameshift`
84	`GGCAGTGCTGCAGGGCTGGGC-------------------------------TTGGCCCG`	`31 bp deleted → frameshift`
84	`GG-------------------------------TCTGCTGGAGCGGCCATGCTTGGCCCG`	`31 bp deleted → frameshift`
82	`GGCAGTGCTGCAGGGCT------------------------------------TGGCCCG`	`36 bp deleted → no frameshift`
80	`GGCAGTGCTGCAGG--------------------------------CCATGCTTGGCCCG`	`32 bp deleted → frameshift`
78	`GGCAGTGCTGCAGGGCTGG-------------------------------------CCCG`	`37 bp deleted → frameshift`
77	`GGCAGTGCTGCAGGGC-----------------------------------------CCG`	`41 bp deleted → frameshift`
76	`GGCAGTGCTGC---------------------------------GGCCATGCTTGGCCCG`	`33 bp deleted → no frameshift`
74	`GGCAGT----------------------------CTGCTGGAGCGGCCATGCTTGGCCCG`	`28 bp deleted → frameshift`
69	`GGC-----------------------------------TGGAGCGGCCATGCTTGGCCCG`	`35 bp deleted → frameshift`
66	`GGCAGTGCTGCAG------------------------------CGGCCATGCTTGGCCCG`	`30 bp deleted → no frameshift`
66	`GGCAGTGCT--------------------------------------------TGGCCCG`	`44 bp deleted → frameshift`
66	`GGC--------------------------------------------CATGCTTGGCCCG`	`44 bp deleted → frameshift`
66	`GGCAGTGCTGC------------------------------------CATGCTTGGCCCG`	`36 bp deleted → no frameshift`
66	`GGCAGTGC------------------------------------GGCCATGCTTGGCCCG`	`36 bp deleted → no frameshift`
64	`GGCAGTGCTGC-----------------------------------------TTGGCCCG`	`41 bp deleted → frameshift`
60	`GGCAGTGCTGCAGGGCTGGGCG--------------------------------------`	`38 bp deleted → frameshift`
57	`GGCAGTGCTGCAGGGCTG---------------------------------CTTGGCCCG`	`33 bp deleted → no frameshift`
57	`GGCAGTG---------------------------------GAGCGGCCATGCTTGGCCCG`	`33 bp deleted → no frameshift`
56	`GGCAGTGC---------------------------------------CATGCTTGGCCCG`	`39 bp deleted → no frameshift`
56	`GG---------------------------------------AGCGGCCATGCTTGGCCCG`	`39 bp deleted → no frameshift`
51	`GGC-----------------------------------------GGCCATGCTTGGCCCG`	`41 bp deleted → frameshift`
48	`GGCAGTGCTGCAGG------------------------------------------CCCG`	`42 bp deleted → no frameshift`
47	`GGCAGTGCTGCA-------------------------------------TGCTTGGCCCG`	`37 bp deleted → frameshift`
45	`GGCAG--------------------------------------CGGCCATGCTTGGCCCG`	`38 bp deleted → frameshift`
40	`GGC------------------------------------------------------CCG`	`54 bp deleted → no frameshift`
40	`GGCAGTGCTGC----------------------------------------------CCG`	`46 bp deleted → frameshift`
34	`GGCAGTGC-------------------------------------------------CCG`	`49 bp deleted → frameshift`
34	`GGC-------------------------------------------------TTGGCCCG`	`49 bp deleted → frameshift`
31	`GGCAGTGCTG---------------------------------------------GCCCG`	`45 bp deleted → no frameshift`
31	`GGCA---------------------------------------------TGCTTGGCCCG`	`45 bp deleted → no frameshift`
27	`GGCAGTG------------------------------------------------GCCCG`	`48 bp deleted → no frameshift`

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