CRISPOR

DNA break repair outcome predictions

Guide: CTGGTACTCGGCGTTCCCGG TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGGTGGCTACCTTTGCTGTGCTGGTACTCGGCGTTCCCGGTGGCGCAGTCTGGGGTGCAGCTCACCTCGATTTCTTCATA`	(Wild-type sequence)
439	`CTTTGCTGTGCTGGTACTCGGCG------------CAGTCTGGGGTGCAGCTCACCTCGA`	`12 bp deleted → no frameshift`
344	`CTTTGCTGTGCTGGTACTCGGCGTTCCCGG---CGCAGTCTGGGGTGCAGCTCACCTCGA`	`3 bp deleted → no frameshift`
296	`CTTTGCTGTGCTGGTACTCGGCGTTCCCG------CAGTCTGGGGTGCAGCTCACCTCGA`	`6 bp deleted → no frameshift`
208	`CTTTGCTGTGCTGGTACTCGGCGTTCCCGG-------------GGTGCAGCTCACCTCGA`	`13 bp deleted → frameshift`
198	`CTTTGCTGTGCTGGTACTCGGCGTTCCCGG--------------GTGCAGCTCACCTCGA`	`14 bp deleted → frameshift`
198	`CTTTGCTGTGCTGGTACTCGGC--------------AGTCTGGGGTGCAGCTCACCTCGA`	`14 bp deleted → frameshift`
193	`CTTTGCTGTGCTGG-------------------CGCAGTCTGGGGTGCAGCTCACCTCGA`	`19 bp deleted → frameshift`
188	`CTTTGCTGTGCTGGTACTCGGCGTTCCCGG---------------TGCAGCTCACCTCGA`	`15 bp deleted → no frameshift`
188	`CTTTGCTGTGCTGGTACTCG---------------CAGTCTGGGGTGCAGCTCACCTCGA`	`15 bp deleted → no frameshift`
164	`CTTTGCTGTGCTGG-----------------------------GGTGCAGCTCACCTCGA`	`29 bp deleted → frameshift`
149	`CTTTGCTGTGCTGGTACTCGGCGTTC--------------TGGGGTGCAGCTCACCTCGA`	`14 bp deleted → frameshift`
141	`CTTTGCTGTGCTGGTACTCGGCGT---------------CTGGGGTGCAGCTCACCTCGA`	`15 bp deleted → no frameshift`
133	`CTTTGCTGTGCTGGTACTCGG----------------------GGTGCAGCTCACCTCGA`	`22 bp deleted → frameshift`
126	`CTTTGCTGTGCTGGTACTCGG-----------------------GTGCAGCTCACCTCGA`	`23 bp deleted → frameshift`
126	`CTTTGCTGTGC-----------------------GCAGTCTGGGGTGCAGCTCACCTCGA`	`23 bp deleted → frameshift`
120	`CTTTGCTGTGCTGGTACTCGG------------------------TGCAGCTCACCTCGA`	`24 bp deleted → no frameshift`
114	`CTTTGCTGTGC-------------------------AGTCTGGGGTGCAGCTCACCTCGA`	`25 bp deleted → frameshift`
109	`CTTTGCTGTGC-------------------------------------AGCTCACCTCGA`	`37 bp deleted → frameshift`
109	`CTTTGCTGTGCTGGTACTCGGC--------------------------AGCTCACCTCGA`	`26 bp deleted → frameshift`
106	`CTTTGCTGTGCTGGT-------------------------------GCAGCTCACCTCGA`	`31 bp deleted → frameshift`
105	`CTTTGCTGTGCTGGTACTC---------------------TGGGGTGCAGCTCACCTCGA`	`21 bp deleted → no frameshift`
99	`CTTTGCTGTGCTGGTACTCGGCGT----------------------GCAGCTCACCTCGA`	`22 bp deleted → frameshift`
99	`CTTTGCTGTG----------------------GCGCAGTCTGGGGTGCAGCTCACCTCGA`	`22 bp deleted → frameshift`
99	`CTTTGCTGTGCTGGTACTCG---------------------------------------A`	`39 bp deleted → no frameshift`
98	`CTTTGCTGTGCTGGTACTCGGCGTTCCC----------------------------TCGA`	`28 bp deleted → frameshift`
98	`CTTTGC----------------------------GCAGTCTGGGGTGCAGCTCACCTCGA`	`28 bp deleted → frameshift`
95	`CTTTGCTGTGCTGGTACT-----------------------GGGGTGCAGCTCACCTCGA`	`23 bp deleted → frameshift`
94	`CTTTGCTGTGCTGGTACTCGGCGTTCC-----------------------------TCGA`	`29 bp deleted → frameshift`
94	`CTTTGCTGTGCTGGTACTCGGC-----------------------------TCACCTCGA`	`29 bp deleted → frameshift`
91	`CTTTGCTGTGCTGGTACTC----------------------------------ACCTCGA`	`34 bp deleted → frameshift`
91	`CTTTGCTG----------------------------------GGGTGCAGCTCACCTCGA`	`34 bp deleted → frameshift`
90	`CTTTGCTGTGCTGGT------------------------CTGGGGTGCAGCTCACCTCGA`	`24 bp deleted → no frameshift`
90	`CTTTGCTG------------------------GCGCAGTCTGGGGTGCAGCTCACCTCGA`	`24 bp deleted → no frameshift`
89	`CTTTGCTGTGCTGGTACTCGGCGTTCCCG------------------------------A`	`30 bp deleted → no frameshift`
89	`CTTTGCTGTGCTGG------------------------------GTGCAGCTCACCTCGA`	`30 bp deleted → no frameshift`
89	`CTTTGC------------------------------AGTCTGGGGTGCAGCTCACCTCGA`	`30 bp deleted → no frameshift`
77	`CTTTGCTGTGCTGGTACTCGGCGTTC---------------------------ACCTCGA`	`27 bp deleted → no frameshift`
77	`CTTTG---------------------------GCGCAGTCTGGGGTGCAGCTCACCTCGA`	`27 bp deleted → no frameshift`
67	`CTTTGCTGTGCT----------------------------------------CACCTCGA`	`40 bp deleted → frameshift`
66	`CTTTGCTGT------------------------------CTGGGGTGCAGCTCACCTCGA`	`30 bp deleted → no frameshift`
66	`CTTTGCTGTGCTGGTACTCGGCG------------------------------------A`	`36 bp deleted → no frameshift`
61	`CTTTGC------------------------------------------AGCTCACCTCGA`	`42 bp deleted → no frameshift`
60	`CTTTGCTGTGCTGGTACTCGGCGTTC--------------------------------GA`	`32 bp deleted → frameshift`
60	`CTTTGCTGTG--------------------------------GGGTGCAGCTCACCTCGA`	`32 bp deleted → frameshift`
54	`CTTTGCTGTGCTG----------------------------------CAGCTCACCTCGA`	`34 bp deleted → frameshift`
52	`CTTTGCT---------------------------------------------CACCTCGA`	`45 bp deleted → no frameshift`
47	`CTTTG-------------------------------------GGGTGCAGCTCACCTCGA`	`37 bp deleted → frameshift`
45	`CTTTGCTGTGCTGGTAC--------------------------------------CTCGA`	`38 bp deleted → frameshift`
42	`CTTTGCTG---------------------------------------CAGCTCACCTCGA`	`39 bp deleted → no frameshift`
42	`CT---------------------------------------GGGGTGCAGCTCACCTCGA`	`39 bp deleted → no frameshift`
31	`CTTTGCTGTGCT---------------------------------------------CGA`	`45 bp deleted → no frameshift`
24	`CTTTGCT--------------------------------------------------CGA`	`50 bp deleted → frameshift`
24	`CT--------------------------------------------------CACCTCGA`	`50 bp deleted → frameshift`
19	`CT-------------------------------------------------------CGA`	`55 bp deleted → frameshift`

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