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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| GGGGATGGAGAGCCGAAAGGACATGGTTATGTTTCTGGATGGGGGTCAGCTTGGCACTCTGGTTGGTAAGAGGGTCTCTA | (Wild-type sequence) | |
| 327 | AGCCGAAAGGACATGGTTATGTTTCTGG----GGGTCAGCTTGGCACTCTGGTTGGTAAG | 4 bp deleted → frameshift |
| 311 | AGCCGAAAGGACATGGTTATGTTTCTGG-----GGTCAGCTTGGCACTCTGGTTGGTAAG | 5 bp deleted → frameshift |
| 296 | AGCCGAAAGGACATGGTTATGTTTCTGG------GTCAGCTTGGCACTCTGGTTGGTAAG | 6 bp deleted → no frameshift |
| 282 | AGCCGAAAGGACATGGTTATGTTTCTGG-------TCAGCTTGGCACTCTGGTTGGTAAG | 7 bp deleted → frameshift |
| 240 | AGCCGAAAGGACATGGTTATGTTTCTGG------------------------TTGGTAAG | 24 bp deleted → no frameshift |
| 224 | AGCCGAAAGGACATGGTTATGTTTCTGG----------------CACTCTGGTTGGTAAG | 16 bp deleted → frameshift |
| 193 | AGCCGAAAGGACATGGT-------------------CAGCTTGGCACTCTGGTTGGTAAG | 19 bp deleted → frameshift |
| 179 | AGCCGAAAGGACATGG----------------GGGTCAGCTTGGCACTCTGGTTGGTAAG | 16 bp deleted → frameshift |
| 170 | AGCCGAAAGGACATGG-----------------GGTCAGCTTGGCACTCTGGTTGGTAAG | 17 bp deleted → frameshift |
| 164 | AGCCGAAAGGACATGGTTATGTTTC------------AGCTTGGCACTCTGGTTGGTAAG | 12 bp deleted → no frameshift |
| 162 | AGCCGAAAGGACATGG------------------GTCAGCTTGGCACTCTGGTTGGTAAG | 18 bp deleted → no frameshift |
| 149 | AGCCGAAAGGACATGGTTATGT--------------CAGCTTGGCACTCTGGTTGGTAAG | 14 bp deleted → frameshift |
| 141 | AGCCGAAAGGACATGGTTATGTTTCT---------------TGGCACTCTGGTTGGTAAG | 15 bp deleted → no frameshift |
| 133 | AGCCGAAAGG----------------------GGGTCAGCTTGGCACTCTGGTTGGTAAG | 22 bp deleted → frameshift |
| 126 | AGCCGAAAGG-----------------------GGTCAGCTTGGCACTCTGGTTGGTAAG | 23 bp deleted → frameshift |
| 123 | AGCCGAAAGGACATGGTTATGTTTCTGG----------------------------TAAG | 28 bp deleted → frameshift |
| 123 | AGCCGAAAGGACATGG----------------------------CACTCTGGTTGGTAAG | 28 bp deleted → frameshift |
| 120 | AGCCGAAAGG------------------------GTCAGCTTGGCACTCTGGTTGGTAAG | 24 bp deleted → no frameshift |
| 115 | AGCCGAAAGGACATGGTT------------------------------------GGTAAG | 36 bp deleted → no frameshift |
| 114 | AGCCGAAAGG-------------------------TCAGCTTGGCACTCTGGTTGGTAAG | 25 bp deleted → frameshift |
| 99 | AGCCGAAAGGACATGGTTATGTTTCT----------------------CTGGTTGGTAAG | 22 bp deleted → frameshift |
| 99 | AGCCGAAAGGACATGGTTATG----------------------GCACTCTGGTTGGTAAG | 22 bp deleted → frameshift |
| 86 | AGCCGAAAGGACA-------------------------GCTTGGCACTCTGGTTGGTAAG | 25 bp deleted → frameshift |
| 84 | AGCCGAAAGGACATGGTTATGTT-------------------------------GGTAAG | 31 bp deleted → frameshift |
| 81 | AGCCGAAAGGACATGGTTATGTTT------------------GGCACTCTGGTTGGTAAG | 18 bp deleted → no frameshift |
| 81 | AGCCGAAAGGACATGGT----------------------------------------AAG | 40 bp deleted → frameshift |
| 78 | AGC-------------------------------------TTGGCACTCTGGTTGGTAAG | 37 bp deleted → frameshift |
| 77 | AGCCGAAAGGACATGGTTATGTT-------------------GGCACTCTGGTTGGTAAG | 19 bp deleted → frameshift |
| 73 | AGCCGAAAGG----------------------------------CACTCTGGTTGGTAAG | 34 bp deleted → frameshift |
| 66 | AGCCGAAAGGACATGGTTATG------------------------------GTTGGTAAG | 30 bp deleted → no frameshift |
| 66 | AGCCGAAAG------------------------------CTTGGCACTCTGGTTGGTAAG | 30 bp deleted → no frameshift |
| 60 | AGCCGAAAGGACATGGTT------------------------GGCACTCTGGTTGGTAAG | 24 bp deleted → no frameshift |
| 57 | AGCCGAAAGGACA---------------------------------CTCTGGTTGGTAAG | 33 bp deleted → no frameshift |
| 54 | AGCCGAAAGGACATGGTTATG----------------------------------GTAAG | 34 bp deleted → frameshift |
| 52 | AGCCGAAAGGACATGGTTATGT-----------------------------------AAG | 35 bp deleted → frameshift |
| 52 | AGCCGAAAGGAC-----------------------------------TCTGGTTGGTAAG | 35 bp deleted → frameshift |
| 48 | AGCCGAAAGG------------------------------------------TTGGTAAG | 42 bp deleted → no frameshift |
| 48 | AGC------------------------------------------ACTCTGGTTGGTAAG | 42 bp deleted → no frameshift |
| 44 | AGCCGAAAGGACATGGTTATGTTT------------------------------GGTAAG | 30 bp deleted → no frameshift |
| 40 | AGCCGAAAGG----------------------------------------------TAAG | 46 bp deleted → frameshift |
| 31 | AGCCGAAAG--------------------------------------------------- | 51 bp deleted → no frameshift |
| 28 | AGCCGAAAGGACATGGTTA---------------------------------------AG | 39 bp deleted → no frameshift |
| 16 | AG---------------------------------------------------------- | 58 bp deleted → frameshift |
| 14 | AGCCGAA----------------------------------------------------G | 52 bp deleted → frameshift |