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Contents:

PCR primers for off-targets of GACTAGCAGGCAGTGCTGCA GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314058_F TCGTCGGCAGCGTCGGAGGCCAGGAGGAGGAG 60.4 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314058_R GTCTCGTGGGCTCGGCTCCAGCAGACCGTGGCTC 62.6 1.00
mm4_intergenic_Sox11|Gm24326_chr12_27350287_F TCGTCGGCAGCGTCGCTGTAGTAACCTCCCACCC 59.4 0.51
mm4_intergenic_Sox11|Gm24326_chr12_27350287_R GTCTCGTGGGCTCGGGCTGGAGTGGCTAAGCAGAA 60.0 0.51
mm4_exon_Ipo7_chr7_110036981_F Primer3: not found at this Tm N.d. 0.45
mm4_exon_Ipo7_chr7_110036981_R Primer3: not found at this Tm N.d. 0.45
mm4_intron_Eif3b_chr5_140440826_F TCGTCGGCAGCGTCGCTGTTCTCATTGGCACTGC 60.1 0.44
mm4_intron_Eif3b_chr5_140440826_R GTCTCGTGGGCTCGGAAAACACCTGCACCTGGCT 60.0 0.44
mm3_intron_Dnajc13_chr9_104204301_F Primer3: not found at this Tm N.d. 0.36
mm3_intron_Dnajc13_chr9_104204301_R Primer3: not found at this Tm N.d. 0.36
mm4_exon_Arhgef4/C230030N03Rik_chr1_34724820_F TCGTCGGCAGCGTCGCAGCGCAGATTGACATCAG 59.9 0.34
mm4_exon_Arhgef4/C230030N03Rik_chr1_34724820_R GTCTCGTGGGCTCGGCAGAGATCCATCCTCTGCGC 60.3 0.34
mm4_intergenic_Gm11404|Gm11221_chr4_69398886_F Primer3: not found at this Tm N.d. 0.32
mm4_intergenic_Gm11404|Gm11221_chr4_69398886_R Primer3: not found at this Tm N.d. 0.32
mm4_intron_Mapt_chr11_104305476_F Primer3: not found at this Tm N.d. 0.31
mm4_intron_Mapt_chr11_104305476_R Primer3: not found at this Tm N.d. 0.31
mm3_intergenic_Gm8349|Arhgef26_chr3_61967767_F Primer3: not found at this Tm N.d. 0.30
mm3_intergenic_Gm8349|Arhgef26_chr3_61967767_R Primer3: not found at this Tm N.d. 0.30
mm4_intron_Dlgap1_chr17_70311100_F Primer3: not found at this Tm N.d. 0.29
mm4_intron_Dlgap1_chr17_70311100_R Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Lrpap1|Adra2c_chr5_35147548_F TCGTCGGCAGCGTCGGACTGAGGCTTGTCGAACA 59.9 0.27
mm4_intergenic_Lrpap1|Adra2c_chr5_35147548_R GTCTCGTGGGCTCGGCCACTGACCTGTCTTCACTCC 59.9 0.27
mm3_intergenic_Spo11|Rae1_chr2_172998516_F Primer3: not found at this Tm N.d. 0.26
mm3_intergenic_Spo11|Rae1_chr2_172998516_R Primer3: not found at this Tm N.d. 0.26
mm4_intron_Cenpm_chr15_82237480_F Primer3: not found at this Tm N.d. 0.25
mm4_intron_Cenpm_chr15_82237480_R Primer3: not found at this Tm N.d. 0.25
mm3_intergenic_Gm11200|Wsb1_chr11_79224617_F Primer3: not found at this Tm N.d. 0.24
mm3_intergenic_Gm11200|Wsb1_chr11_79224617_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_94312127_F TCGTCGGCAGCGTCAGGTCTGTTGGTAGCATGGC 60.0 0.23
mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_94312127_R GTCTCGTGGGCTCGGTCCACCCCATGACACAGAGA 60.1 0.23
mm3_intron_Anxa4_chr6_86793121_F Primer3: not found at this Tm N.d. 0.22
mm3_intron_Anxa4_chr6_86793121_R Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Syf2|Gm16225_chr4_134979699_F TCGTCGGCAGCGTCGCCTAGAGAGTGTCAGTGCC 59.8 0.21
mm4_intergenic_Syf2|Gm16225_chr4_134979699_R GTCTCGTGGGCTCGGTAGCACGTCCCGAAACAAGT 59.6 0.21
mm4_intergenic_Gm22331|Gm24937_chr1_113670247_F TCGTCGGCAGCGTCGCTCAAATGCCATATGCCACA 59.5 0.20
mm4_intergenic_Gm22331|Gm24937_chr1_113670247_R GTCTCGTGGGCTCGGACCATGCTCAAAACTAATTCTGGA 58.6 0.20
mm4_intron_Prex2_chr1_11138340_F Primer3: not found at this Tm N.d. 0.20
mm4_intron_Prex2_chr1_11138340_R Primer3: not found at this Tm N.d. 0.20
mm2_exon_Upk2_chr9_44452797_F TCGTCGGCAGCGTCACGTGGGTCAGCCTTTATTGT 59.9 0.20
mm2_exon_Upk2_chr9_44452797_R GTCTCGTGGGCTCGGGAGTCCACTCCTGACAGTGC 60.0 0.20
mm4_exon_Eprs_chr1_185372908_F Primer3: not found at this Tm N.d. 0.19
mm4_exon_Eprs_chr1_185372908_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm13982|Gm13985_chr2_117535949_F TCGTCGGCAGCGTCGGCTGGGATGATGAGAAGCA 59.8 0.18
mm4_intergenic_Gm13982|Gm13985_chr2_117535949_R GTCTCGTGGGCTCGGGTCCCTCCATCAAGATGCTGA 59.5 0.18
mm4_intron_Ccser2_chr14_36907864_F TCGTCGGCAGCGTCCCCTTTGTCATTCCCACTTTGG 59.7 0.17
mm4_intron_Ccser2_chr14_36907864_R GTCTCGTGGGCTCGGGGTGTATGATGTGAACCGCC 58.9 0.17
mm4_exon_Gm22867_chr2_44429009_F Primer3: not found at this Tm N.d. 0.17
mm4_exon_Gm22867_chr2_44429009_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Stac|Arpp21_chr9_111719466_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Stac|Arpp21_chr9_111719466_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Stac|Arpp21_chr9_111719436_F TCGTCGGCAGCGTCTGAGTTGTGCTTGACCACGT 60.1 0.17
mm4_intergenic_Stac|Arpp21_chr9_111719436_R GTCTCGTGGGCTCGGGAGAGCAGACCTGACAGCAC 60.3 0.17
mm4_intron_Iqsec3_chr6_121448668_F TCGTCGGCAGCGTCTGACACATGCCATGGCTGAT 60.0 0.16
mm4_intron_Iqsec3_chr6_121448668_R GTCTCGTGGGCTCGGGTGGAAACCTCAGCCTCCAA 59.8 0.16
mm4_intron_Card11_chr5_140885114_F TCGTCGGCAGCGTCTCACGCAGGAAAACCCAGAG 60.2 0.16
mm4_intron_Card11_chr5_140885114_R GTCTCGTGGGCTCGGACTCTGGGCCAAGCACATC 60.0 0.16
mm4_intergenic_Abra|Gm24751_chr15_41874732_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Abra|Gm24751_chr15_41874732_R Primer3: not found at this Tm N.d. 0.16
mm3_intergenic_Med27|Rapgef1_chr2_29576786_F Primer3: not found at this Tm N.d. 0.16
mm3_intergenic_Med27|Rapgef1_chr2_29576786_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm16203|Sgcz_chr8_38594149_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm16203|Sgcz_chr8_38594149_R Primer3: not found at this Tm N.d. 0.16
mm4_intron_Arsb_chr13_93798311_F TCGTCGGCAGCGTCGGTCCTGATGGTGGTTGGAG 60.0 0.15
mm4_intron_Arsb_chr13_93798311_R GTCTCGTGGGCTCGGTAAATGATGGAGGGAGGGGA 57.0 0.15
mm4_intergenic_Gm11268|Gm11269_chr4_82663997_F TCGTCGGCAGCGTCTACCTCTTCCTGCTCAGCCT 59.9 0.15
mm4_intergenic_Gm11268|Gm11269_chr4_82663997_R GTCTCGTGGGCTCGGTCTGTATGTCTGGGGCCTGA 59.9 0.15
mm4_intergenic_Stac|Arpp21_chr9_111719406_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Stac|Arpp21_chr9_111719406_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Tha1|Gm11725_chr11_117880979_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Tha1|Gm11725_chr11_117880979_R Primer3: not found at this Tm N.d. 0.14
mm2_intron_Mamdc2_chr19_23445624_F TCGTCGGCAGCGTCGGGGCTGTTTAACGGTCACA 60.5 0.14
mm2_intron_Mamdc2_chr19_23445624_R GTCTCGTGGGCTCGGTCCCATCCAAAATCCTGGGC 60.0 0.14
mm4_intron_Adam12_chr7_134087035_F TCGTCGGCAGCGTCTTGCACATCTCCAGAAGGCC 60.3 0.13
mm4_intron_Adam12_chr7_134087035_R GTCTCGTGGGCTCGGTGCTTTCCAAGGGGTTGTGT 60.0 0.13
mm3_exon_Sirt6_chr10_81625092_F TCGTCGGCAGCGTCTGTGTTACACGGGCAACACT 60.1 0.12
mm3_exon_Sirt6_chr10_81625092_R GTCTCGTGGGCTCGGCAGGATCTGGGAAGGGTCCT 60.3 0.12
mm4_intergenic_Gm24295|Mef2c_chr13_82614148_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm24295|Mef2c_chr13_82614148_R Primer3: not found at this Tm N.d. 0.12
mm4_exon_Gm8217_chr1_121520645_F TCGTCGGCAGCGTCCCGAAAGCGAAGAAGGAAGC 59.5 0.12
mm4_exon_Gm8217_chr1_121520645_R GTCTCGTGGGCTCGGGAAGGTGGGTGACATTCGGA 59.6 0.12
mm4_intergenic_Gm8793|Nkain2_chr10_31911983_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm8793|Nkain2_chr10_31911983_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Bfsp1_chr2_143859446_F TCGTCGGCAGCGTCAAAGGGCTGGAAGGACAACC 60.1 0.12
mm4_intron_Bfsp1_chr2_143859446_R GTCTCGTGGGCTCGGGACGCTCACCCTACAGCAAG 60.7 0.12
mm3_intergenic_Ipmk|Gm22937_chr10_71710393_F TCGTCGGCAGCGTCACGGCTGTGGAATGGACTAC 59.7 0.11
mm3_intergenic_Ipmk|Gm22937_chr10_71710393_R GTCTCGTGGGCTCGGGAGACCACTGCCACATGTCA 59.9 0.11
mm4_intron_Adamts2_chr11_50631923_F TCGTCGGCAGCGTCGGTTCCAGTGTGACAGAGGG 59.9 0.11
mm4_intron_Adamts2_chr11_50631923_R GTCTCGTGGGCTCGGGCCAACCAAGAGCCAAAAGG 59.9 0.11
mm4_intergenic_4933401P06Rik|Ppp2r2c_chr5_36890940_F TCGTCGGCAGCGTCTCTTTCTCCTGGCACCTCCT 60.1 0.11
mm4_intergenic_4933401P06Rik|Ppp2r2c_chr5_36890940_R GTCTCGTGGGCTCGGCCCTATCTGTGGCTGAGAGG 58.9 0.11
mm2_intergenic_Gm25200|Cdh13_chr8_118233999_F TCGTCGGCAGCGTCTTCCACCTGCAGCAGACATC 60.3 0.10
mm2_intergenic_Gm25200|Cdh13_chr8_118233999_R GTCTCGTGGGCTCGGTGCAGAAAACCTTCCTGGCT 59.8 0.10
mm4_intron_Trabd2b_chr4_114504811_F TCGTCGGCAGCGTCGGCCTCCTTGGTTGCTGAAA 60.8 0.10
mm4_intron_Trabd2b_chr4_114504811_R GTCTCGTGGGCTCGGTGCCTCTCTCCTCTGAAGCA 60.2 0.10
mm4_intergenic_Gm8730|Cdh5_chr8_103992033_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm8730|Cdh5_chr8_103992033_R Primer3: not found at this Tm N.d. 0.09
mm3_exon_Klc4_chr17_46644369_F Primer3: not found at this Tm N.d. 0.09
mm3_exon_Klc4_chr17_46644369_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Nucb1_chr7_45502469_F TCGTCGGCAGCGTCCACCATCAGAGCTGACCCTG 60.1 0.09
mm4_intron_Nucb1_chr7_45502469_R GTCTCGTGGGCTCGGCTGTTTGCTGGTAGCCCCTT 60.2 0.09
mm4_intron_Abcb10_chr8_123979196_F TCGTCGGCAGCGTCTCTGAGGCTCCTGGTAACCA 59.8 0.09
mm4_intron_Abcb10_chr8_123979196_R GTCTCGTGGGCTCGGGTCTGCAGTGCTACCCAGAG 60.1 0.09
mm4_intergenic_Calcr|Tfpi2_chr6_3827589_F TCGTCGGCAGCGTCGCGGAGGGCTTAGTGCTAT 59.2 0.09
mm4_intergenic_Calcr|Tfpi2_chr6_3827589_R GTCTCGTGGGCTCGGAATGGGGAGCACTGGAATGG 60.0 0.09
mm4_intron_Itgb3_chr11_104611603_F TCGTCGGCAGCGTCAGGTGAGACGTGGCATTTGT 59.8 0.09
mm4_intron_Itgb3_chr11_104611603_R GTCTCGTGGGCTCGGGGGTGGAGAACTGCAAAGGA 59.8 0.09
mm4_intron_Chd7_chr4_8864985_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Chd7_chr4_8864985_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm16036|Sdk1_chr5_141653097_F TCGTCGGCAGCGTCGAGACACCCCATCCCCATTG 60.1 0.08
mm4_intergenic_Gm16036|Sdk1_chr5_141653097_R GTCTCGTGGGCTCGGCCATGACTCAGCACGTGGAT 60.1 0.08
mm4_intergenic_9330185C12Rik|Cntnap5a_chr1_114921485_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_9330185C12Rik|Cntnap5a_chr1_114921485_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_4930520K10Rik|Cdk19_chr10_40382502_F TCGTCGGCAGCGTCATCAGCATATAATTGTTGGTCAGG 57.1 0.07
mm4_intergenic_4930520K10Rik|Cdk19_chr10_40382502_R GTCTCGTGGGCTCGGTCTGACAGGCCTAGAACTTGC 59.7 0.07
mm4_intergenic_Asah2|Sgms1_chr19_32120957_F TCGTCGGCAGCGTCGGTTTCTCCCACTGCAGAGG 60.3 0.07
mm4_intergenic_Asah2|Sgms1_chr19_32120957_R GTCTCGTGGGCTCGGCCAGCCAGTGACTAAGACCA 59.0 0.07
mm4_exon_Gramd1b/1700110K17Rik_chr9_40333640_F TCGTCGGCAGCGTCGTCTCCGTCCTGGTTCTGTG 60.0 0.07
mm4_exon_Gramd1b/1700110K17Rik_chr9_40333640_R GTCTCGTGGGCTCGGCCATCTCATTCCCCGTCTCC 59.6 0.07
mm3_intron_Spon1_chr7_113979677_F TCGTCGGCAGCGTCTAGAGCATCCTTCCCTGGGT 59.6 0.07
mm3_intron_Spon1_chr7_113979677_R GTCTCGTGGGCTCGGTAGGGGAGGACTTTGGGAGG 59.9 0.07
mm4_intron_Casz1_chr4_148941138_F TCGTCGGCAGCGTCGTGATCTCGTCAGCCGGAAG 60.5 0.07
mm4_intron_Casz1_chr4_148941138_R GTCTCGTGGGCTCGGAGGGGCTCAGGTTTAACACG 59.9 0.07
mm4_intron_Usp12_chr5_146753172_F TCGTCGGCAGCGTCGGAAACATTGCTGTAACAACTAGC 58.8 0.07
mm4_intron_Usp12_chr5_146753172_R GTCTCGTGGGCTCGGTTGGCTAGAGAAAGGCTGGC 60.0 0.07
mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_94454780_F TCGTCGGCAGCGTCGCTGGGCATAACAGTATGATGG 59.1 0.07
mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_94454780_R GTCTCGTGGGCTCGGAGAAGTCTCATGGAGAATGAAAGT 57.6 0.07
mm4_intergenic_9330159F19Rik|Echdc1_chr10_29281526_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_9330159F19Rik|Echdc1_chr10_29281526_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Magi2|Gm22755_chr5_20246128_F TCGTCGGCAGCGTCCACTTCATGGCATCCAGGGT 60.0 0.06
mm4_intergenic_Magi2|Gm22755_chr5_20246128_R GTCTCGTGGGCTCGGAGGGAGTAGAGGGCAATGGA 59.6 0.06
mm4_intergenic_Dgkg|Gm22481_chr16_22589811_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Dgkg|Gm22481_chr16_22589811_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Arhgap15|Gm22867_chr2_44405885_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Arhgap15|Gm22867_chr2_44405885_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Tmc1|Zfand5_chr19_20960789_F TCGTCGGCAGCGTCACTGAATTCCACAGTCAAAACATCC 59.9 0.06
mm3_intergenic_Tmc1|Zfand5_chr19_20960789_R GTCTCGTGGGCTCGGACCTCGGCAAAAGAAGTGGT 59.8 0.06
mm4_intergenic_Gm11754|Gm11755_chr11_119121976_F TCGTCGGCAGCGTCTTTGAGAACTGCCCCAAGCC 60.8 0.06
mm4_intergenic_Gm11754|Gm11755_chr11_119121976_R GTCTCGTGGGCTCGGGGACCACCCTAGAAACCTTTCC 60.2 0.06
mm4_intergenic_Rbms3|Gm26614_chr9_117671347_F TCGTCGGCAGCGTCTCTGTTGTGGGCTTCAGTCC 59.8 0.06
mm4_intergenic_Rbms3|Gm26614_chr9_117671347_R GTCTCGTGGGCTCGGAGTTCTCTCATTTGTTGTTTCTCCA 58.8 0.06
mm4_intron_March1_chr8_66463017_F TCGTCGGCAGCGTCGCAAGGTGTTTGTGGAGTAGC 59.7 0.06
mm4_intron_March1_chr8_66463017_R GTCTCGTGGGCTCGGGAGTCACTTTGGCTTGCGTG 60.0 0.06
mm4_intergenic_March1|Gm15354_chr8_66318240_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_March1|Gm15354_chr8_66318240_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm9381|Ndst4_chr3_125093401_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm9381|Ndst4_chr3_125093401_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Lcorl|Gm7931_chr5_46202306_F TCGTCGGCAGCGTCTGGTCAGATTTAAGGCTCCCAG 59.7 0.06
mm4_intergenic_Lcorl|Gm7931_chr5_46202306_R GTCTCGTGGGCTCGGGCTCAAGTTCAGGGCTGTCA 60.2 0.06
mm4_intron_Rnaset2b_chr17_7001924_F TCGTCGGCAGCGTCCCATCACCTCTTGCCTTGGT 59.9 0.05
mm4_intron_Rnaset2b_chr17_7001924_R GTCTCGTGGGCTCGGGTGACGGTCATTCACCCTGT 59.9 0.05
mm4_intergenic_Gm1604A|Rnaset2a_chr17_8124802_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm1604A|Rnaset2a_chr17_8124802_R Primer3: not found at this Tm N.d. 0.05
mm4_exon_Abhd17c_chr7_84147769_F TCGTCGGCAGCGTCAGCCATTTGAACACCCACCA 60.1 0.05
mm4_exon_Abhd17c_chr7_84147769_R GTCTCGTGGGCTCGGGAGTGAGTGCTCTGTTGACCA 59.9 0.05
mm4_intergenic_Plxdc2|Gm24933_chr2_16846598_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Plxdc2|Gm24933_chr2_16846598_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Mir365-2|9130204K15Rik_chr11_79750098_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Mir365-2|9130204K15Rik_chr11_79750098_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm15930|Gm15925_chr7_3777180_F TCGTCGGCAGCGTCGCCCTGTGTGTCACATGACT 60.2 0.05
mm4_intergenic_Gm15930|Gm15925_chr7_3777180_R GTCTCGTGGGCTCGGATGGGGAGGATGGTGTGACT 60.2 0.05
mm4_intergenic_Gm10693|Gm14548_chr7_3875015_F TCGTCGGCAGCGTCGCCCTGTGTGTCACATGACT 60.2 0.05
mm4_intergenic_Gm10693|Gm14548_chr7_3875015_R GTCTCGTGGGCTCGGATGGGGAGGATGGTGTGACT 60.2 0.05
mm4_intron_Tfdp1_chr8_13368318_F TCGTCGGCAGCGTCCCTTCATGCTGGTGTGGGAT 60.0 0.05
mm4_intron_Tfdp1_chr8_13368318_R GTCTCGTGGGCTCGGCTCCCCATCCCCAGACAGTA 60.0 0.05
mm4_intergenic_Tmod1|Tmod1/Tstd2_chr4_46108856_F TCGTCGGCAGCGTCAGGAGCACTGCTGGGGTATA 60.0 0.05
mm4_intergenic_Tmod1|Tmod1/Tstd2_chr4_46108856_R GTCTCGTGGGCTCGGATGGCCTGGACTCTGAGTCT 59.9 0.05
mm4_intergenic_Snx12|Gm24061_chrX_101121945_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Snx12|Gm24061_chrX_101121945_R Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Lama2|Gm10145_chr10_27746336_F Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Lama2|Gm10145_chr10_27746336_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Col18a1_chr10_77152776_F TCGTCGGCAGCGTCGCCTTCTGCAGTTTGACTCC 59.1 0.04
mm4_intron_Col18a1_chr10_77152776_R GTCTCGTGGGCTCGGCTCATGTCTCACCCATGCGT 60.1 0.04
mm4_intron_Acat1_chr9_53602339_F TCGTCGGCAGCGTCCCTGCAGGGATTGAGCTCC 60.1 0.04
mm4_intron_Acat1_chr9_53602339_R GTCTCGTGGGCTCGGGGGGCAGCTTGCTTATGGTA 60.1 0.04
mm4_intron_Atp6v0d2_chr4_19894090_F TCGTCGGCAGCGTCAAACAACCACGCTCAAAGCC 59.8 0.04
mm4_intron_Atp6v0d2_chr4_19894090_R GTCTCGTGGGCTCGGAGGTCCAAGGAGAGAGAAGAA 57.4 0.04
mm4_intergenic_Lipt2|Kcne3_chr7_100163492_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Lipt2|Kcne3_chr7_100163492_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Dag1_chr9_108206907_F Primer3: not found at this Tm N.d. 0.04
mm4_exon_Dag1_chr9_108206907_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Neto2_chr8_85679545_F TCGTCGGCAGCGTCAAGACACAAGTGAGCCCTGG 59.8 0.04
mm4_intron_Neto2_chr8_85679545_R GTCTCGTGGGCTCGGACCTACCCTCTCCCATCACA 59.2 0.04
mm4_intergenic_Hnrnpa1|Nfe2_chr15_103245919_F TCGTCGGCAGCGTCAGTGACCATCGCAAGGAAGG 60.0 0.03
mm4_intergenic_Hnrnpa1|Nfe2_chr15_103245919_R GTCTCGTGGGCTCGGTGTTCTTACCTAGCAGCATAGCA 59.5 0.03
mm4_intron_Fbln7_chr2_128870500_F TCGTCGGCAGCGTCAGGGTGAATGGATGGAGGGA 59.9 0.03
mm4_intron_Fbln7_chr2_128870500_R GTCTCGTGGGCTCGGTGACTCTACTTCTCTACAACCAATC 57.8 0.03
mm4_intergenic_Stk31|Npy_chr6_49588922_F TCGTCGGCAGCGTCCGCAGGTTAGACAGAGCACT 59.7 0.03
mm4_intergenic_Stk31|Npy_chr6_49588922_R GTCTCGTGGGCTCGGCCCTGGTTGGTCATCCCATG 60.3 0.03
mm4_intron_Plekho2_chr9_65561249_F TCGTCGGCAGCGTCAGTACAGTGCACTTGGCCTG 60.2 0.03
mm4_intron_Plekho2_chr9_65561249_R GTCTCGTGGGCTCGGGCCTGGTCATGCAAATGAGC 60.1 0.03
mm3_intergenic_4930429F24Rik|Filip1_chr9_79810727_F Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_4930429F24Rik|Filip1_chr9_79810727_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm14274|Mc3r_chr2_172228373_F TCGTCGGCAGCGTCAGATGAAGGTTGAGTCACCGA 58.7 0.03
mm4_intergenic_Gm14274|Mc3r_chr2_172228373_R GTCTCGTGGGCTCGGTAGCCTTTCTGTGCAGCTGG 60.3 0.03
mm4_intergenic_Epas1|Tmem247_chr17_86870362_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Epas1|Tmem247_chr17_86870362_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Mylk3|4921524J17Rik_chr8_85398245_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Mylk3|4921524J17Rik_chr8_85398245_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm14515|Gm14513_chrX_11639609_F TCGTCGGCAGCGTCATGCTCTGGGGCTCTCACTA 60.0 0.03
mm4_intergenic_Gm14515|Gm14513_chrX_11639609_R GTCTCGTGGGCTCGGCTCTAGTCACAGCTGGTCACA 59.1 0.03
mm4_intergenic_Gm25802|Gm12464_chr3_100364220_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm25802|Gm12464_chr3_100364220_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm23905|Zfp36l2_chr17_83993548_F TCGTCGGCAGCGTCAGAAGCCAATGAGGAGCCAC 60.0 0.03
mm4_intergenic_Gm23905|Zfp36l2_chr17_83993548_R GTCTCGTGGGCTCGGCAATACCTCCCTTCCCCAGC 59.8 0.03
mm4_intergenic_Pitx1|H2afy_chr13_55841126_F TCGTCGGCAGCGTCCATGTTGGCATGGTGTGTGC 60.6 0.02
mm4_intergenic_Pitx1|H2afy_chr13_55841126_R GTCTCGTGGGCTCGGGGCCAGGCCCCACTTATATG 60.2 0.02
mm4_intergenic_Gm25758|Adamts3_chr5_89834568_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25758|Adamts3_chr5_89834568_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25778|Ppapdc1a_chr7_129172070_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25778|Ppapdc1a_chr7_129172070_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Gm15268_chrX_141627960_F TCGTCGGCAGCGTCTTCCACCATCTTGGCTCCTG 59.6 0.02
mm4_exon_Gm15268_chrX_141627960_R GTCTCGTGGGCTCGGAGACCTTGCTTGGAACCAACA 60.0 0.02
mm3_intergenic_Tigit|Drd3_chr16_43762067_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Tigit|Drd3_chr16_43762067_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Trank1_chr9_111363936_F TCGTCGGCAGCGTCGGGGTCCTGATGCTCTCTTT 59.0 0.02
mm4_exon_Trank1_chr9_111363936_R GTCTCGTGGGCTCGGTGTCTCAGGCACATGAGCAG 60.0 0.02
mm4_intron_Ipp_chr4_116522339_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Ipp_chr4_116522339_R Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Vmn2r-ps77|Vmn2r73_chr7_85840706_F TCGTCGGCAGCGTCTGCCTTGCTCTCAGCTTTGA 59.8 0.02
mm3_intergenic_Vmn2r-ps77|Vmn2r73_chr7_85840706_R GTCTCGTGGGCTCGGCCAGCCAGCAGAGGAAAACA 60.5 0.02
mm4_exon_Dtx1_chr5_120694747_F TCGTCGGCAGCGTCCCAGGATGGCGTTGGAGG 60.1 0.02
mm4_exon_Dtx1_chr5_120694747_R GTCTCGTGGGCTCGGTTCCCAAGTCGCAATCCTGG 60.3 0.02
mm4_intron_Osbpl5_chr7_143692341_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Osbpl5_chr7_143692341_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Cebpa|Slc7a10_chr7_35152079_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Cebpa|Slc7a10_chr7_35152079_R Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Zhx2|Gm16006_chr15_57707022_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Zhx2|Gm16006_chr15_57707022_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm3833|Fhit_chr14_10369782_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm3833|Fhit_chr14_10369782_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ndfip1|Spry4_chr18_38492115_F TCGTCGGCAGCGTCGACCCTATCGCTACACCACC 59.6 0.02
mm4_intergenic_Ndfip1|Spry4_chr18_38492115_R GTCTCGTGGGCTCGGGGCTTCTTGGCCCAGATAGT 59.4 0.02
mm4_intron_Abcc2_chr19_43812725_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Abcc2_chr19_43812725_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Prdm2|Gm13050_chr4_143222872_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Prdm2|Gm13050_chr4_143222872_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Igfbp2|Igfbp5_chr1_72856974_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Igfbp2|Igfbp5_chr1_72856974_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_9930013L23Rik_chr7_83981314_F TCGTCGGCAGCGTCGATGCTCAGTCTTGGGGTGT 59.6 0.02
mm4_intron_9930013L23Rik_chr7_83981314_R GTCTCGTGGGCTCGGTTGTGCCTCCTGGTAGCATG 60.0 0.02
mm4_intergenic_Kcnq1|KCNQ1DN_chr7_143437123_F TCGTCGGCAGCGTCAGTGCCCGTGAAATCTTCCA 59.6 0.01
mm4_intergenic_Kcnq1|KCNQ1DN_chr7_143437123_R GTCTCGTGGGCTCGGATGCTGGAGAGAGGCAGGTA 60.0 0.01
mm4_intergenic_Agtr1b|Gm7488_chr3_20518662_F TCGTCGGCAGCGTCCTTGCCTCCACACTCTCAGG 60.0 0.01
mm4_intergenic_Agtr1b|Gm7488_chr3_20518662_R GTCTCGTGGGCTCGGCCAGAAAAGGTGGGGCTCAT 59.9 0.01
mm4_intergenic_Gm12287|Gm12288_chr11_63770376_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm12287|Gm12288_chr11_63770376_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm14576|Gm5385_chrX_41031552_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm14576|Gm5385_chrX_41031552_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Brip1_chr11_86098835_F TCGTCGGCAGCGTCGCAGGTAAGGGCAGGAGATG 60.1 0.01
mm4_intron_Brip1_chr11_86098835_R GTCTCGTGGGCTCGGTGGCACTGTTGATCTTTTGCC 59.6 0.01
mm4_intergenic_Mtmr12|1810049J17Rik/Golph3_chr15_12294669_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Mtmr12|1810049J17Rik/Golph3_chr15_12294669_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Nkain3_chr4_20161466_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nkain3_chr4_20161466_R Primer3: not found at this Tm N.d. 0.00
mm3_intron_Tmod3_chr9_75505247_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Tmod3_chr9_75505247_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Syne3_chr12_104994548_F TCGTCGGCAGCGTCCTCAAAGCCGCTCTGCTCT 60.0 0.00
mm4_intron_Syne3_chr12_104994548_R GTCTCGTGGGCTCGGCAGAGGCAAGCTAGGCTGAG 60.1 0.00
mm4_intergenic_Edn3|Gm14617_chr2_174815050_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Edn3|Gm14617_chr2_174815050_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ptgds_chr2_25467703_F TCGTCGGCAGCGTCTCGGACAGACTGACACCTCC 60.8 0.00
mm4_intron_Ptgds_chr2_25467703_R GTCTCGTGGGCTCGGAGCAAGTGAGGGCAACTAGG 59.6 0.00
mm4_intron_Gm15558_chr16_10680029_F TCGTCGGCAGCGTCTCTCAGCCTTCAGGGAGACT 59.5 0.00
mm4_intron_Gm15558_chr16_10680029_R GTCTCGTGGGCTCGGCCAGAGAGGCCTTGTTTCCT 59.3 0.00
mm4_intron_Mrvi1_chr7_110943162_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mrvi1_chr7_110943162_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Kalrn_chr16_34433432_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Kalrn_chr16_34433432_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Pcnt_chr10_76429700_F TCGTCGGCAGCGTCTGACTTTGTTCACAGTGCAGC 59.5 0.00
mm4_intron_Pcnt_chr10_76429700_R GTCTCGTGGGCTCGGGGGACCCATGGTTTCACAGT 59.8 0.00
mm4_intron_Lef1_chr3_131136751_F TCGTCGGCAGCGTCGATGCCAGCTATGCCTCCTT 59.8 0.00
mm4_intron_Lef1_chr3_131136751_R GTCTCGTGGGCTCGGTCAGATTGGGAGAGGGACCC 60.3 0.00
mm4_intron_Diap3_chr14_86912732_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Diap3_chr14_86912732_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_n-R5-8s1|Mex3c_chr18_73562486_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_n-R5-8s1|Mex3c_chr18_73562486_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ube2g2|Gm19402_chr10_77688052_F TCGTCGGCAGCGTCCCTTGCATTGTGAGGTGAGC 59.4 0.00
mm4_intergenic_Ube2g2|Gm19402_chr10_77688052_R GTCTCGTGGGCTCGGCAGGAAAGCACACGTTCGTG 60.0 0.00
mm4_intergenic_Gm6569/Mroh5|Gm7935_chr15_74000589_F TCGTCGGCAGCGTCGCTAGAGCGGATTTCAGCCT 59.8 0.00
mm4_intergenic_Gm6569/Mroh5|Gm7935_chr15_74000589_R GTCTCGTGGGCTCGGCAGTGAGAGCCACCCATCTT 59.3 0.00
mm4_intergenic_Gm23881|Gm3534_chr14_48364737_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23881|Gm3534_chr14_48364737_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm13903|Kcna4_chr2_107266434_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm13903|Kcna4_chr2_107266434_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm11355|Prl_chr13_26994958_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm11355|Prl_chr13_26994958_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_F630040K05Rik|Vegfa_chr17_45865591_F TCGTCGGCAGCGTCCCCCTCCCTCAGGTTTCAAA 59.2 0.00
mm4_intergenic_F630040K05Rik|Vegfa_chr17_45865591_R GTCTCGTGGGCTCGGGACTCCTCCAACTGCTTGCT 59.9 0.00
mm4_intergenic_4933428C19Rik|Gm12391_chr4_40582759_F TCGTCGGCAGCGTCATGTCCAACACGTCGCTTCA 60.2 0.00
mm4_intergenic_4933428C19Rik|Gm12391_chr4_40582759_R GTCTCGTGGGCTCGGCAACATGGTCCCTGAGCTGT 59.9 0.00
mm4_exon_Fcgbp_chr7_28086147_F TCGTCGGCAGCGTCATGTCCTGTCCAGCCAACAG 59.9 0.00
mm4_exon_Fcgbp_chr7_28086147_R GTCTCGTGGGCTCGGGCACAGCTTCTGAGTTGCAG 59.7 0.00
mm4_exon_Cyp2s1_chr7_25805057_F TCGTCGGCAGCGTCGTGTGGGAGGGATGTCCAAG 60.0 0.00
mm4_exon_Cyp2s1_chr7_25805057_R GTCTCGTGGGCTCGGCCTCTGCTCTGGTTTCTCCG 60.1 0.00
mm3_intron_St6gal1_chr16_23326657_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_St6gal1_chr16_23326657_R Primer3: not found at this Tm N.d. 0.00
mm3_intron_Fer1l5_chr1_36409474_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Fer1l5_chr1_36409474_R Primer3: not found at this Tm N.d. 0.00
mm3_intron_Casp8_chr1_58824613_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Casp8_chr1_58824613_R Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Lrrtm1|Ctnna2_chr6_77490331_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Lrrtm1|Ctnna2_chr6_77490331_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314058 GGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAG
mm4_intergenic_Sox11|Gm24326_chr12_27350287 GCTGTAGTAACCTCCCACCCCCACCCCAGTAACCTATCCAGAGATAGAAGAGTAAACCCTGCAGCACTGTCCTCTAGTAA
AATGTTTTGCAAGAATTTCTGCTTAGCCACTCCAGC
mm4_intron_Eif3b_chr5_140440826 GCTGTTCTCATTGGCACTGCTGTGAGAGTTTCTTGTCATAGACCAGTGGGTGACGAGCAGGCCCAGGATCCTTGCAGCAT
TGTTTGCTAGCC
ATCTTTTCTGCTGGAGGGGGAACACAGCCAGGTGCAGGTGTTTT
mm4_exon_Arhgef4/C230030N03Rik_chr1_34724820 GCAGCGCAGATTGACATCAGTTTTGCCCTCCCCTGCAGCTCTGCCTGCCATTTGACCTATGAGAACCAAGGCATGCCCTG
CAGACCCACAAGCCCCAAACCCAGGAGTCCCATGGCTGGGGCGCAGAGGATGGATCTCTG
mm4_intergenic_Lrpap1|Adra2c_chr5_35147548 GGACTGAGGCTTGTCGAACAGGGGAGCCAAACCCAGGCCAGAGTATTCTGGAGCCAGGTGGGACCTGCTCTGCCCAGCAG
CACTGCCTGCTGGGT
GGAGTGAAGACAGGTCAGTGG
mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_94312127 AGGTCTGTTGGTAGCATGGCAGTATGCAGGTCTCTGTGTGTCTTCTTCCTCCCATGCTGCACTGCCTGGTGGTAGTGGGA
CTCTCTGTGTCATGGGGTGGA
mm4_intergenic_Syf2|Gm16225_chr4_134979699 GCCTAGAGAGTGTCAGTGCCATGTCTCTGGCAAGCGCGGGCCCTGCAGTTCTGCCTCCTAGCCATTCCTCAGGAAAACTG
GTGGAGTTGGCTTCCGGGTGTTTCCCTCTTAACTTGTTTCGGGACGTGCTA
mm4_intergenic_Gm22331|Gm24937_chr1_113670247 GCTCAAATGCCATATGCCACAAGAATCCTGAACCTTACAGGTCAACCATATAGCACTGCCTTCTATTCCAAAGATGGTTT
TCCAGAATTAGTTTTGAGCATGGT
mm2_exon_Upk2_chr9_44452797 ACGTGGGTCAGCCTTTATTGTGAGAATTAAGTACAGTTAAGAGATAAATCAGTGTTGGCAGGATTGCAGTGGGGCTAGCA
GGGAGTGCTGCAAGG
TGAGACAGGGAGCAGGGAGGGGGCACTGTCAGGAGTGGACTC
mm4_intergenic_Gm13982|Gm13985_chr2_117535949 GGCTGGGATGATGAGAAGCACAGAGAGAAAGGAGAAAGTTCACCCTAGTCAAATTCTTCTTCAAAGACTGGTGTCATAAA
TAGCAGCCAGTGCTGCTGGG
GTGACCTCTCCCTTTAGCTTCAGCATCTTGATGGAGGGAC
mm4_intron_Ccser2_chr14_36907864 CCCTTTGTCATTCCCACTTTGGACTCATTTTCACAAATTCTAAATGCAATACTGCCATAATACGAGTTAAAGTTATACCA
GACCTACAGGCGGTGCTGCAAGGGATGGCGGTTCACATCATACACC
mm4_intergenic_Stac|Arpp21_chr9_111719436 TGAGTTGTGCTTGACCACGTGTGGCAGGCAGTGCTGCTGGGTGACTGTGCGTGGCAGGCAGTGCTGCTGGGTGACTGTGC
GTGGCAGGCAGTGCTGCTGGGTGACTGTGCGTGGCAGGCAGTGCTGTCAGGTCTGCTCTC
mm4_intron_Iqsec3_chr6_121448668 TGACACATGCCATGGCTGATACGAGCCTAAAGTCTGGAAAGCCTTCTCCTTTCCAGGTGTTGGCTAGTTGGCAGTGCTGC
TGGG
GTTCAGTCTTTATTGAACATTATGCTTTGGAGCCTCTTGGAGGCTGAGGTTTCCAC
mm4_intron_Card11_chr5_140885114 TCACGCAGGAAAACCCAGAGGTGAGAATGAGAATCGAGTCATCACCCGGGCAGCAAGCAGTGCTGCAGGGGGCTGGGCCA
GGCGGGGAGGCAGGGCTGCCTGCCTGTCAGCGGACCGGATGTGCTTGGCCCAGAGT
mm4_intron_Arsb_chr13_93798311 GGTCCTGATGGTGGTTGGAGAACCTTCTAGCCTTAGATAATCATTCTTTTCATGAGGCTACCATGCAGCACAGACTACCA
GTC
TCCCCTCCCTCCATCATTTA
mm4_intergenic_Gm11268|Gm11269_chr4_82663997 TACCTCTTCCTGCTCAGCCTCTGTCCTCCCCCTGGAGCACTGCCTGCTTTCCTCATCAGGAATCCTTCTCAGCAGGCTCA
GGCCCCAGACATACAGA
mm2_intron_Mamdc2_chr19_23445624 GGGGCTGTTTAACGGTCACACTGGTGTAGAGATGCCAGTGCTGTGCAGCACTTCCTGCCAGTCTGGGAGGCCCTAGCACT
CAGCAGGCAGCAGCATGCTCGCCCAGGATTTTGGATGGGA
mm4_intron_Adam12_chr7_134087035 TTGCACATCTCCAGAAGGCCAGATAGATCTAGACACTTGCAAACCATACTACAAAGTCTTCCCTTGAGGGAAAGGTGAAA
CTTCTCCATGCAGCAGTGCCTCCTAGATGCTCACACAACCCCTTGGAAAGCA
mm3_exon_Sirt6_chr10_81625092 TGTGTTACACGGGCAACACTCACGTCTTACACTTGGGACATTCCTCTACAAACATGTTTCCGTGCAGCTCTGCCAGCTTG
TC
CCTGCAGAAGAAGATGCCAGTAAGGACCCTTCCCAGATCCTG
mm4_exon_Gm8217_chr1_121520645 CCGAAAGCGAAGAAGGAAGCTCCTGCCCATCCCAAAGCCAAAGTGAAAGCCTTGAAAGCCAAGAAGGCAGTGCTGAAAGG
CATCCACAGCCACAAAAAGAAGAAGATCCGAATGTCACCCACCTTC
mm4_intron_Bfsp1_chr2_143859446 AAAGGGCTGGAAGGACAACCAAAGCCAAGCAACACTGCCTGCCAGTTCCAAGTGGGCTGAGCAGCTTGCTGTAGGGTGAG
CGTC
mm3_intergenic_Ipmk|Gm22937_chr10_71710393 ACGGCTGTGGAATGGACTACCTTATGACAGGTCTTTGCAGCACTGTCTGGCAGTCTTCAGCGAGAGAGTATAGATGTGAG
ACTGACATGTGGCAGTGGTCTC
mm4_intron_Adamts2_chr11_50631923 GGTTCCAGTGTGACAGAGGGCTCCTAGATACAGAGTCCCTGGGCCAGTGTGACAGAGGGCTCCTAGATACAGAGTGCCTG
GGCTCCCAGGCAGTGCTGAAGGGCCAACTGTGTGTCCCTTTTGGCTCTTGGTTGGC
mm4_intergenic_4933401P06Rik|Ppp2r2c_chr5_36890940 TCTTTCTCCTGGCACCTCCTCGCCTTCTTCCCAGGCAGGGAAGAATAGCAGCCACTGCTGCGGGGTTATTTCAAGGGGAT
CCCCATGGAAACGGGGAGTGGGCGGCTCCTCTCAGCCACAGATAGGG
mm2_intergenic_Gm25200|Cdh13_chr8_118233999 TTCCACCTGCAGCAGACATCAGCGCCACTCCCTGCAGCACTCCCAGCTAGTCAATACAAACAGATGTGAAGTAAACAGAG
AACTGGCAGGGAGGGTGTCTTGGTAGCCAGGAAGGTTTTCTGCA
mm4_intron_Trabd2b_chr4_114504811 GGCCTCCTTGGTTGCTGAAAATAAACTGAGCCAGGCCCCCAGCAGTGCTGCCTGCTGGTCTGCTTGCCTGAAGAGGAACA
GGGCTGTTTCTGCTTCAGAGGAGAGAGGCA
mm4_intron_Nucb1_chr7_45502469 CACCATCAGAGCTGACCCTGGCCTCCACTGCTCACAGCACTCCAGGGCTCACCAGCGTCTCTGCTGGGTACAAGGCCCTG
CAACACTGCCTGCCCATC
AGCCCCAAGGGGCTACCAGCAAACAG
mm4_intron_Abcb10_chr8_123979196 TCTGAGGCTCCTGGTAACCACAGTGATAAGAGTAGCACTCAGTGCTGAAAGGCTCTGGGTAGCACTGCAGAC
mm4_intergenic_Calcr|Tfpi2_chr6_3827589 GCGGAGGGCTTAGTGCTATGACAATGAGGCAGAGCTGCAAGGTTCTGTGGTAAGATGTTAACCCTCTAAGAATATAACTG
AAGCATTTGTAGGAGGGTTATTTAGAATCACTGATATATTCCATTCCAGTGCTCCCCATT
mm4_intron_Itgb3_chr11_104611603 AGGTGAGACGTGGCATTTGTGGCTTGGAAGAGCATCCAATGGGACAAGGAGGCAGTGCAGCGAGGAGAGGGATCTGAATG
GGACTCTAGAATTCCCGACGAAAACTCTTCCTTTGCAGTTCTCCACCC
mm4_intergenic_Gm16036|Sdk1_chr5_141653097 GAGACACCCCATCCCCATTGCCTTCCTATGAGGCTGTCATTTCATTCCGAACAGCACTGCCTGCTCCTCCATCCACGTGC
TGAGTCATGG
mm4_intergenic_4930520K10Rik|Cdk19_chr10_40382502 ATCAGCATATAATTGTTGGTCAGGCCTTGCAGTGCTGCCTACAAGTCCAAGTATTTACAAAGCAAGTTCTAGGCCTGTCA
GA
mm4_intergenic_Asah2|Sgms1_chr19_32120957 GGTTTCTCCCACTGCAGAGGTTGCTCCCACATTCAGTATACCTTACAGCACTGCCTGGGACTCCTCTTACTACCTATGTG
CCTCTGGTCTTAGTCACTGGCTGG
mm4_exon_Gramd1b/1700110K17Rik_chr9_40333640 GTCTCCGTCCTGGTTCTGTGGTGTTGGTGAGCGGGACCGCTTCCGGAGGATGGGAGAGCAGGCTGGCGTGCTGCGGTTGG
AGTTACTGGCAGTGCTGCAGGG
GCAGATGGAGACGGGGAATGAGATGG
mm3_intron_Spon1_chr7_113979677 TAGAGCATCCTTCCCTGGGTCGCTCATCAGCATGTATCCTCACTAGGGTAGGAGAAGCTCTCTGCAGCACTGCATTCTAG
AC
CAGAGTGCCTACTGGAGAGCTATTGCTATTCATCCTTTCCTCCCAAAGTCCTCCCCTA
mm4_intron_Casz1_chr4_148941138 GTGATCTCGTCAGCCGGAAGAGCCGCTGACCAGCAGCCAGTGCTCCTGGGGCCCCCTGCCTGTTCGGCCCACTGCCCGCC
CTTTGGGTGTAAAGGAACGAGGGTACTAGATTTTGTGCCCGTGTTAAACCTGAGCCCCT
mm4_intron_Usp12_chr5_146753172 GGAAACATTGCTGTAACAACTAGCAATCAAACATTGCCCTGCACCACTGACTGCTGGTTCAGGCAATGAAAAAGGCCCAC
ACCACAGCCAGCCTTTCTCTAGCCAA
mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_94454780 GCTGGGCATAACAGTATGATGGGAAGCATCTCCCTGTTGCACTGCATGCAAGTCACTTTCATTCTCCATGAGACTTCT
mm4_intergenic_Magi2|Gm22755_chr5_20246128 CACTTCATGGCATCCAGGGTACAATGAATGGAAAGGAAGCAGAAGTGACTGACAGGCAGTTCTTCAAGGATGTTCCTCCA
TTGCCCTCTACTCCCT
mm3_intergenic_Tmc1|Zfand5_chr19_20960789 ACTGAATTCCACAGTCAAAACATCCTTTCTCTGCAGCACTGACAGCCAGTCTTTGCCCCAAGCACTTGTTTATCACCACT
TCTTTTGCCGAGGT
mm4_intergenic_Gm11754|Gm11755_chr11_119121976 TTTGAGAACTGCCCCAAGCCAACAAGGGGGTTTACCTGCTTCAAACAAGGACGTTTTCAATATGGTTGGTACAAGACCCT
TGGGGCACTGGCTGCTTGTC
TCTGGTACTTGGAAAGGTTTCTAGGGTGGTCC
mm4_intergenic_Rbms3|Gm26614_chr9_117671347 TCTGTTGTGGGCTTCAGTCCCTTGATAGATTTTCATGCTTAAATGGCATATTGGATAGTCCCAGCAACACTGCCTGGTAC
TC
TAATTTTGGAGAAACAACAAATGAGAGAACT
mm4_intron_March1_chr8_66463017 GCAAGGTGTTTGTGGAGTAGCAGAGACTGAGGGTCCCTGCAGAACTGAGTGCTAGTAGGACATCTCCCAGGACACACGCA
AGCCAAAGTGACTC
mm4_intergenic_Lcorl|Gm7931_chr5_46202306 TGGTCAGATTTAAGGCTCCCAGCCTAGTACTACTGCCTGCTAGTCCACTGCCCCTCAGTCCCCCTAATCTGCACTACCAT
GATCAAGGCATTATAGTTTGACAGCCCTGAACTTGAGC
mm4_intron_Rnaset2b_chr17_7001924 CCATCACCTCTTGCCTTGGTGTGTATGACTCCTACCCTGGATGCTTTGCTTAGCCCTGGAGCAATGCCTCCTATTCAAGT
AACAGTGCATGGTACAGGGTGAATGACCGTCAC
mm4_exon_Abhd17c_chr7_84147769 AGCCATTTGAACACCCACCATTTTCGTTGAATGGCTGTAGCCACTTTTTGCAACTCTGCAGCACTGACTGCCTGCCACTG
GTCAACAGAGCACTCACTC
mm4_intergenic_Gm15930|Gm15925_chr7_3777180 GCCCTGTGTGTCACATGACTGTTGTGTCTCTTTTCTGGCCAGCTGTTTTTCTTGACCTGACAGTATGTGATGAGTTATGG
CATTGTACTGGTAAGACTTGCAGCCAGTGCTGTGAGGTCAGTCACACCATCCTCCCCAT
mm4_intergenic_Gm10693|Gm14548_chr7_3875015 GCCCTGTGTGTCACATGACTGTTGTGTCTCTTTTCTGGCCAGCTGTTTTTCTTGACCTGACAGTATGTGATGAGTTATGG
CATTGTACTGGTAAGACTTGCAGCCAGTGCTGTGAGGTCAGTCACACCATCCTCCCCAT
mm4_intron_Tfdp1_chr8_13368318 CCTTCATGCTGGTGTGGGATGTGTCAACGTGACTGGCAGGCAGTACTGGTGGGGGACGTAAAGCTCTTACTGTCTGGGGA
TGGGGAG
mm4_intergenic_Tmod1|Tmod1/Tstd2_chr4_46108856 AGGAGCACTGCTGGGGTATACCAGATGACTAGCCAGGGGTGCTGCAGGGATATATACAGGAATCTTTCTGAATGAGACAG
ACAAGTTCTGAGACTCAGAGTCCAGGCCAT
mm4_intron_Col18a1_chr10_77152776 GCCTTCTGCAGTTTGACTCCTTGGCAAAGCAGGCAGTTCTGCAGGGAACCAGGAAGATGGCAGGACAACAGCAGACCCAC
TAAAATCTGCTCTGACATACAGCGTACGCATGGGTGAGACATGAG
mm4_intron_Acat1_chr9_53602339 CCTGCAGGGATTGAGCTCCCGTGCCCTGGGCTTACCCCCCAGCAGCAATGCATGCTTGTCGAGAAGCGAGGGGATCACTT
GGACATATTCCTTAGGGTTCCGTAAGGACAACAAGACCTCTACCATAAGCAAGCTGCCCC
mm4_intron_Atp6v0d2_chr4_19894090 AAACAACCACGCTCAAAGCCGGGGCTTCAGTCCCTTTATCCGGTTCAGATAAAACCACCAGCTCACGACAGACAGCCCTT
CCAGCACTGCCCTCTGGTC
TCAGATTTCTTCTCTCTCCTTGGACCT
mm4_intron_Neto2_chr8_85679545 AAGACACAAGTGAGCCCTGGCATGTTTTTAAGGTTAAGATGACTTTCAGTGGAGAGGGAGGTAAGAAGGAAAGAACAGCA
AGTCAGTGAGGAAGAAAAGCAGGCAGTGGTTCACGGGCTTGTGATGGGAGAGGGTAGGT
mm4_intergenic_Hnrnpa1|Nfe2_chr15_103245919 AGTGACCATCGCAAGGAAGGAGGCAGACGAGAGTAAGGCACACCTGACTCTTAGGACTAGCAGGCAGAGCCAGAAGGAAA
GGTTTATTGCTATGCTGCTAGGTAAGAACA
mm4_intron_Fbln7_chr2_128870500 AGGGTGAATGGATGGAGGGAACAGACAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNACAAAAGATTGGTTGTAGAGAAGTAGAGTCA
mm4_intergenic_Stk31|Npy_chr6_49588922 CGCAGGTTAGACAGAGCACTGCCTGGGAGCACTGCCTGATTGTCACTAGGCTCATGGGATGACCAACCAGGG
mm4_intron_Plekho2_chr9_65561249 AGTACAGTGCACTTGGCCTGGCCCCCTTAGCATCCTCATTATATAACTTCCTCCTGGCAGCACTGCCTCCTCCTCATACA
GGACCCTAGGTCCCCCTCCATGCCAGCCACAGCCAAATGCTCATTTGCATGACCAGGC
mm4_intergenic_Gm14274|Mc3r_chr2_172228373 AGATGAAGGTTGAGTCACCGAAAGATGTAGACGAGAACTTCCTGACCAGCAGGCACTGAGGCAAGGCCAGCTGCACAGAA
AGGCTA
mm4_intergenic_Gm14515|Gm14513_chrX_11639609 ATGCTCTGGGGCTCTCACTAAGTGCCCAGACTGGCCCCAGACATTGGCCTTGGAGATCTGCCTGCTAGACTGTGACCAGC
TGTGACTAGAG
mm4_intergenic_Gm23905|Zfp36l2_chr17_83993548 AGAAGCCAATGAGGAGCCACGGTGTGCCTGGGAGCACTGGGAGGCAGTGCTGGAGGGACCAGTGCTGGGGAAGGGAGGTA
TTG
mm4_intergenic_Pitx1|H2afy_chr13_55841126 CATGTTGGCATGGTGTGTGCTCGCCTAGCAGGCAGTTCTGATGGGTGAGGTGCCCTGGGGGTCTTGGTGAGGAGCCCACA
TGCCTAGCTCGGGACTCAGCATCATATAAGTGGGGCCTGGCC
mm4_exon_Gm15268_chrX_141627960 TTCCACCATCTTGGCTCCTGTGGAGGCCTGCTGGGAACAGGACTTCTAACAGCAAGTATGTCTAGAAGGCTGTGGTGCAA
GG
CCATTTTTGTTGGTTCCAAGCAAGGTCT
mm4_exon_Trank1_chr9_111363936 GGGGTCCTGATGCTCTCTTTTCTGCCTCTTTCTCCCATTCTACTCCCTTCCAGCACTGCCTGAGGGTCTCCAAGACAGCC
AGGACAGGCCGCTGCTCATGTGCCTGAGACA
mm3_intergenic_Vmn2r-ps77|Vmn2r73_chr7_85840706 TGCCTTGCTCTCAGCTTTGAACTGTATTCAAATGATTGTCCTGCAGCACTGCCTGCTAAGGCAATAATTGCTCTGAGTTC
TGTTTTCCTCTGCTGGCTGG
mm4_exon_Dtx1_chr5_120694747 CCAGGATGGCGTTGGAGGCGGCGCGCGTGCTGTTGACCAGCAGGCACTGCTGACAGGAGCAGGGCTGACCCGAGCTGGCT
CCCACGGGCCAGGATTGCGACTTGGGAA
mm4_intergenic_Ndfip1|Spry4_chr18_38492115 GACCCTATCGCTACACCACCGTACTCCTTAAGGTCCCTGGAGTGACCAGCAGGCTGTGCTCCCAGGCCAGGTCTGCCTTT
TTGATGCCCATTTAGGAAGATACTATCTGGGCCAAGAAGCC
mm4_intron_9930013L23Rik_chr7_83981314 GATGCTCAGTCTTGGGGTGTACTCAAGGTTTAGCCTAAACCTAGGGGAAATCCTCCTTCTACTTCCATGCAGCAAGGCCT
GGAAGTC
AGGCTTTGCCCATCCTGCATAGCTGCATGCTACCAGGAGGCACAA
mm4_intergenic_Kcnq1|KCNQ1DN_chr7_143437123 AGTGCCCGTGAAATCTTCCATCTGGTCTAGGTTTGTCCTTCCAACACTGCCTGCCAATCCTGCATCGCAGCTGGTTCTTA
GTGATCACCCAGGCCCTACCTGCCTCTCTCCAGCAT
mm4_intergenic_Agtr1b|Gm7488_chr3_20518662 CTTGCCTCCACACTCTCAGGCCTAGTTCACATTGCCAGTCTGGACTGCCTGCTAGTCTCCAAATGAGCCCCACCTTTTCT
GG
mm4_intron_Brip1_chr11_86098835 GCAGGTAAGGGCAGGAGATGGGTTACTAGCATGCAGTGGGGCAAGGCAGAGATTTGACTAAAGATTATACACTGTAGTAG
GCAAAAGATCAACAGTGCCA
mm4_intron_Syne3_chr12_104994548 CTCAAAGCCGCTCTGCTCTGCGCTGCTCACCGGCTTCCCACCGCCTGCCTTCCAACACTGGCTGCTTGTCGCAGGAACCC
ATGCAGCTCCCAGGCTCAGCCTAGCTTGCCTCTG
mm4_intron_Ptgds_chr2_25467703 TCGGACAGACTGACACCTCCATTTATTTCCTTGAAGCCCCCTGGACTAGCAGGTAGTAGGGCAAGGCTAAGCTCGCCCTA
GTTGCCCTCACTTGCT
mm4_intron_Gm15558_chr16_10680029 TCTCAGCCTTCAGGGAGACTGTTAAGCGTTTATGTCCATTTTTGGTCATTTGTCACCTAGTGCAGCCAGCCAGCACCCCC
TGCTAGTC
CAGGCTGGGGGTGCCCCTTCTCTGAAGGAAACAAGGCCTCTCTGG
mm4_intron_Pcnt_chr10_76429700 TGACTTTGTTCACAGTGCAGCTTCAACCGTGCAGCCATGCCTGCGAGCCGCATGCCTCCTCCCTGCCTGAGACCTGAACT
ATAGCTATATGTCTCTATACCATCTGCCCCGTGTGTGCTCACTGTGAAACCATGGGTCCC
mm4_intron_Lef1_chr3_131136751 GATGCCAGCTATGCCTCCTTGACTGAAAAGGGAACAGGTTAGGGGGAGGAGCCGGCCCTGGGATTCCACCTTGCATCCCT
GCCTGCCTGTC
AGGGTCCCTCTCCCAATCTGA
mm4_intergenic_Ube2g2|Gm19402_chr10_77688052 CCTTGCATTGTGAGGTGAGCTTGTAAATGAACCCCTGAAGGACCCCCGTGCAGGCCTGCCTGGGAGTCGTGAGGACGGCC
ATGCTTCCGGGCCTGGGATGAATCACGAACGTGTGCTTTCCTG
mm4_intergenic_Gm6569/Mroh5|Gm7935_chr15_74000589 GCTAGAGCGGATTTCAGCCTTCAGAGGCCTGAGGGCTGTGGCAGGCAGTGGGCGGTGCCCCCGTGGGCAGCATGTTCTTC
CCTGCAGCCCTGCCTGCTCCTG
GCCCTTCTAGAAGATGGGTGGCTCTCACTG
mm4_intergenic_F630040K05Rik|Vegfa_chr17_45865591 CCCCTCCCTCAGGTTTCAAATGCCTATCATCTTCTATCCCAGGCTATGTCTGACTAACAGGAAGGGCTGCTGGGGTCCAG
TTTACCCATGAAATACAGGTTAGTCCAAGCTGAAGAAGCAAGCAGTTGGAGGAGTC
mm4_intergenic_4933428C19Rik|Gm12391_chr4_40582759 ATGTCCAACACGTCGCTTCAGCTGCAACCCTGCAACCCTGCCAGATAGTCCTTCATTTCTACAGCTCAGGGACCATGTTG
mm4_exon_Fcgbp_chr7_28086147 ATGTCCTGTCCAGCCAACAGCTGCTATGATCCTTGCAGCCCTGCCTGCCCGCCTTCCTGCAACTCAGAAGCTGTGC
mm4_exon_Cyp2s1_chr7_25805057 GTGTGGGAGGGATGTCCAAGAACGGACAGGCAGGGAGGGCTGCAAGGCTATCTCTCAGGCCAGATAGAGCTTGTGTATTC
TTGGGGCTTTGGTGTAGAGGGGAAAGGCAGGCGGAGAAACCAGAGCAGAGG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.