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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313629_F | TCGTCGGCAGCGTCTTGCAGCAGGAGGATGACAG | 60.0 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313629_R | GTCTCGTGGGCTCGGAGATGGCAGTGGGGAGCTA | 59.9 | 1.00 |
| mm3_intergenic_Gramd1a|G630030J09Rik_chr7_31148062_F | Primer3: not found at this Tm | N.d. | 0.53 |
| mm3_intergenic_Gramd1a|G630030J09Rik_chr7_31148062_R | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intergenic_Gpr133|Sfswap_chr5_129454576_F | TCGTCGGCAGCGTCCACACCTCTCTGGCTGTGAG | 60.0 | 0.47 |
| mm4_intergenic_Gpr133|Sfswap_chr5_129454576_R | GTCTCGTGGGCTCGGCTCCATGAGTTTGGGGTGGG | 60.3 | 0.47 |
| mm4_intergenic_Gm5356|Gm26331_chr8_89282395_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Gm5356|Gm26331_chr8_89282395_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm3_exon_Scly_chr1_91310517_F | TCGTCGGCAGCGTCAGCCATCACCAAATCCACCC | 60.3 | 0.27 |
| mm3_exon_Scly_chr1_91310517_R | GTCTCGTGGGCTCGGCCTGTTGTAGAGGCGTGGAG | 60.1 | 0.27 |
| mm4_intron_Dock2_chr11_34724424_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Dock2_chr11_34724424_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm3_intron_Stim1_chr7_102309552_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm3_intron_Stim1_chr7_102309552_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Nfkbia_chr12_55491965_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Nfkbia_chr12_55491965_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_exon_Ralbp1_chr17_65885357_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_exon_Ralbp1_chr17_65885357_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_E4f1_chr17_24448758_F | TCGTCGGCAGCGTCGAGAAAGGCAGTGGGGACTC | 60.0 | 0.19 |
| mm4_intron_E4f1_chr17_24448758_R | GTCTCGTGGGCTCGGAAGCTCTGACAGTCAGCAGG | 59.6 | 0.19 |
| mm4_intron_8430427H17Rik_chr2_153465301_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_8430427H17Rik_chr2_153465301_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm14266|Gm26489_chr2_171278122_F | TCGTCGGCAGCGTCAGATGTCCTAAGTCTCCACCT | 57.2 | 0.18 |
| mm4_intergenic_Gm14266|Gm26489_chr2_171278122_R | GTCTCGTGGGCTCGGGCCAAATTGGATGGGGTTAGG | 59.2 | 0.18 |
| mm3_intergenic_Gm4262|Litaf_chr16_11026821_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intergenic_Gm4262|Litaf_chr16_11026821_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm26903|Gm25300_chr9_75958574_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm26903|Gm25300_chr9_75958574_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm26685|Dpt_chr1_164783213_F | TCGTCGGCAGCGTCTGAGGTAAGATGCCAGAGTCC | 58.8 | 0.17 |
| mm4_intergenic_Gm26685|Dpt_chr1_164783213_R | GTCTCGTGGGCTCGGTCCTCTGCTTTCTGCCTCAC | 59.6 | 0.17 |
| mm4_intergenic_Azin1|Atp6v1c1_chr15_38539378_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Azin1|Atp6v1c1_chr15_38539378_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm6419|Rtdr1_chr10_74929333_F | TCGTCGGCAGCGTCAGCACTGCCATGTGGGATAC | 60.1 | 0.16 |
| mm4_intergenic_Gm6419|Rtdr1_chr10_74929333_R | GTCTCGTGGGCTCGGGGGGCAAGAATGTTGTGTGG | 59.6 | 0.16 |
| mm4_intron_Gm15326_chr13_111870648_F | TCGTCGGCAGCGTCTGCTGTTCCTCCCACTGTTC | 59.8 | 0.16 |
| mm4_intron_Gm15326_chr13_111870648_R | GTCTCGTGGGCTCGGGCCACCCAACACTCTGTACA | 59.8 | 0.16 |
| mm4_intron_Fbxl7_chr15_26668685_F | TCGTCGGCAGCGTCGCATGCAGCATCAGCCAATG | 60.8 | 0.15 |
| mm4_intron_Fbxl7_chr15_26668685_R | GTCTCGTGGGCTCGGTGCCCCAGAAAAGCTATGGG | 60.0 | 0.15 |
| mm4_intron_Ank2_chr3_127363524_F | TCGTCGGCAGCGTCGTGTGGGGTTTAGGCTCTGG | 60.3 | 0.15 |
| mm4_intron_Ank2_chr3_127363524_R | GTCTCGTGGGCTCGGCCAAACAAGGGGAAGGGTGA | 59.8 | 0.15 |
| mm4_intergenic_Cspg5|mmu-mir-6236_chr9_110263353_F | TCGTCGGCAGCGTCCAGCTGGGCTTACTGTCTGT | 59.6 | 0.13 |
| mm4_intergenic_Cspg5|mmu-mir-6236_chr9_110263353_R | GTCTCGTGGGCTCGGTATATGCCCTAGCCCCTCCC | 59.9 | 0.13 |
| mm4_intergenic_Gpr12|Usp12_chr5_146664189_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gpr12|Usp12_chr5_146664189_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm10220|Gm7361_chr5_26206874_F | TCGTCGGCAGCGTCTTTGTGTCTGACTATCACCACT | 57.0 | 0.11 |
| mm4_intergenic_Gm10220|Gm7361_chr5_26206874_R | GTCTCGTGGGCTCGGCCTTTATATGCAACTAGCCAACCA | 59.1 | 0.11 |
| mm3_intergenic_Tsen54|Llgl2_chr11_115823764_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_Tsen54|Llgl2_chr11_115823764_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Zmynd8|BC046401_chr2_165854815_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Zmynd8|BC046401_chr2_165854815_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Gm23069_chr6_141714917_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Gm23069_chr6_141714917_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Afap1_chr5_35907365_F | TCGTCGGCAGCGTCTGTGAGAGGCTTCTGTTTGCA | 60.1 | 0.09 |
| mm4_intron_Afap1_chr5_35907365_R | GTCTCGTGGGCTCGGCCCTCCCATCCCCTCTTACT | 59.7 | 0.09 |
| mm4_intergenic_Pik3r1|Gm25808_chr13_102113958_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Pik3r1|Gm25808_chr13_102113958_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Adck4_chr7_27255614_F | TCGTCGGCAGCGTCATGCCCAGACAAGTCACAGG | 59.9 | 0.08 |
| mm4_intron_Adck4_chr7_27255614_R | GTCTCGTGGGCTCGGCAGAGCAGGGAGAGGAAGGA | 60.3 | 0.08 |
| mm4_intron_Tbk1_chr10_121552914_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Tbk1_chr10_121552914_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Bmp5_chr9_75815747_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Bmp5_chr9_75815747_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Stk25|Bok_chr1_93666647_F | TCGTCGGCAGCGTCTTGCTTGTTCTCTCAGGGCC | 60.2 | 0.08 |
| mm4_intergenic_Stk25|Bok_chr1_93666647_R | GTCTCGTGGGCTCGGGGAGGAGTCAAAGGCAGCTT | 59.9 | 0.08 |
| mm4_intron_Epb4.1l2_chr10_25447698_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Epb4.1l2_chr10_25447698_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Nav2_chr7_49079169_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Nav2_chr7_49079169_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Vps37c_chr19_10714215_F | TCGTCGGCAGCGTCCCATGGTGCTTCCCAGACAT | 60.0 | 0.07 |
| mm4_exon_Vps37c_chr19_10714215_R | GTCTCGTGGGCTCGGTCATTACTAAGCAGGCTCCAGC | 60.1 | 0.07 |
| mm4_intergenic_Prkar2b|Pik3cg_chr12_32061509_F | TCGTCGGCAGCGTCAATCCCACGCACCTTTCTCC | 60.3 | 0.06 |
| mm4_intergenic_Prkar2b|Pik3cg_chr12_32061509_R | GTCTCGTGGGCTCGGCACCACGCACGTTTCTCTTG | 60.0 | 0.06 |
| mm4_intron_Cux2_chr5_121973231_F | TCGTCGGCAGCGTCGGACTTCCTTCCTCCTCCCT | 59.9 | 0.04 |
| mm4_intron_Cux2_chr5_121973231_R | GTCTCGTGGGCTCGGTGTGGACATCTTCCCACAGC | 59.9 | 0.04 |
| mm4_intergenic_Gm25278|Csmd1_chr8_16957706_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm25278|Csmd1_chr8_16957706_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm11217|Gm23950_chr4_64280771_F | TCGTCGGCAGCGTCAGCAGGAGGGGAGTATTTGA | 57.7 | 0.04 |
| mm4_intergenic_Gm11217|Gm23950_chr4_64280771_R | GTCTCGTGGGCTCGGACTGTGTGGCACTGGTTTCA | 60.0 | 0.04 |
| mm4_intron_Ccdc23_chr4_119198623_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Ccdc23_chr4_119198623_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Mvb12b_chr2_33740060_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Mvb12b_chr2_33740060_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Clic5_chr17_44205453_F | TCGTCGGCAGCGTCACATAACCACCTGGCGCG | 60.4 | 0.03 |
| mm4_intron_Clic5_chr17_44205453_R | GTCTCGTGGGCTCGGCAAAGGGCGAGGGCAAGG | 61.0 | 0.03 |
| mm4_intergenic_Gm17388|Htr1d_chr4_136408020_F | TCGTCGGCAGCGTCTACTCCGTGAAGCTGCCAAG | 60.0 | 0.03 |
| mm4_intergenic_Gm17388|Htr1d_chr4_136408020_R | GTCTCGTGGGCTCGGCCAACTGTGCGATGCTTTCC | 60.1 | 0.03 |
| mm4_exon_Pde11a_chr2_75991107_F | TCGTCGGCAGCGTCTGCGGTTCAACCAAAGCAAG | 59.8 | 0.03 |
| mm4_exon_Pde11a_chr2_75991107_R | GTCTCGTGGGCTCGGGCCGGGGAAGACAGACTGTA | 61.2 | 0.03 |
| mm3_exon_Lama5_chr2_180198981_F | TCGTCGGCAGCGTCCGCTGACAGAGAGGGAAGTG | 60.1 | 0.03 |
| mm3_exon_Lama5_chr2_180198981_R | GTCTCGTGGGCTCGGTAGCCAACAGCCTCTGTGAC | 59.6 | 0.03 |
| mm3_intergenic_Gm21957|4930533L02Rik_chr7_125255096_F | TCGTCGGCAGCGTCTGTCTGCCAAAAGCCTAAGGA | 59.5 | 0.02 |
| mm3_intergenic_Gm21957|4930533L02Rik_chr7_125255096_R | GTCTCGTGGGCTCGGCAGAGGGAGGCAAGTCCAAG | 60.0 | 0.02 |
| mm4_intergenic_Gm25279|1700025G04Rik_chr1_151856847_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm25279|1700025G04Rik_chr1_151856847_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm22735|5930412G12Rik_chr5_128449238_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm22735|5930412G12Rik_chr5_128449238_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_A230028O05Rik|Tprg_chr16_25254838_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_A230028O05Rik|Tprg_chr16_25254838_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fdft1_chr14_63163600_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fdft1_chr14_63163600_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_D230002A01Rik_chr8_91804755_F | TCGTCGGCAGCGTCCCTTTCCTCCCAAGGCTCAG | 60.0 | 0.01 |
| mm4_exon_D230002A01Rik_chr8_91804755_R | GTCTCGTGGGCTCGGTGACTGAGGAAAACGAGCGT | 59.6 | 0.01 |
| mm4_exon_Ptpn12_chr5_20998598_F | TCGTCGGCAGCGTCTAGCTTCTGTTGAGGTCGGC | 59.7 | 0.01 |
| mm4_exon_Ptpn12_chr5_20998598_R | GTCTCGTGGGCTCGGTCAGCCTTCCCAACCGTTAC | 59.9 | 0.01 |
| mm4_intron_1700034P13Rik_chr1_9768868_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_1700034P13Rik_chr1_9768868_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Klc1_chr12_111785327_F | TCGTCGGCAGCGTCTGGATGCACGCTGAAGAGAG | 60.1 | 0.00 |
| mm4_intron_Klc1_chr12_111785327_R | GTCTCGTGGGCTCGGAGAGAAGTCAGGTGAGTGAGTG | 59.1 | 0.00 |
| mm4_intron_Scube1_chr15_83681015_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Scube1_chr15_83681015_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ccdc112|Gm24617_chr18_46334067_F | TCGTCGGCAGCGTCGGGCAGACATTTGAGCAGGA | 60.3 | 0.00 |
| mm4_intergenic_Ccdc112|Gm24617_chr18_46334067_R | GTCTCGTGGGCTCGGGCTGGCATTGAGTTATCCCG | 59.0 | 0.00 |
| mm4_exon_Fam65a_chr8_105622074_F | TCGTCGGCAGCGTCCCACTGGGGTTGGCAGAAT | 59.9 | 0.00 |
| mm4_exon_Fam65a_chr8_105622074_R | GTCTCGTGGGCTCGGCCAAGGGATGTGATGAGGCA | 59.7 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313629 |
TTGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAGGAGCGCT CGCATCACAAGGTGACCCTAGCTCCCCACTGCCATCT |
| mm4_intergenic_Gpr133|Sfswap_chr5_129454576 |
CACACCTCTCTGGCTGTGAGCAGTCTCTCGTTCTTCCATAGGAAGCTGCCATGAGACATCTCAACACTGTGCTGCACAGC TTCTGGAGGGGAAAGACCCACCCCAAACTCATGGAG |
| mm3_exon_Scly_chr1_91310517 |
AGCCATCACCAAATCCACCCCCAGGCTTCCACCCAGAGGCTGTGTGGAACACCTCAGGCCTCATCATGGTGTCAACAGAG ACCCTAAAGGGACCCTGGAGTCTCCACGCCTCTACAACAGG |
| mm4_intron_E4f1_chr17_24448758 |
GAGAAAGGCAGTGGGGACTCAGACAGGTGTGCCACACCTCCTCAGGAAGGCAATGAGCTCAGAGCCCCCTTCCCAAGGTT GGCTTCCTCAACAGACCTTTCTGCTTCCTGCCCCTGCTGACTGTCAGAGCTT |
| mm4_intergenic_Gm14266|Gm26489_chr2_171278122 |
AGATGTCCTAAGTCTCCACCTTACTGTGCCTAACAGCCTCTTGGGGGACTCTTAATTCTTCAGGGTGAAGTACACTTTCA TCCTAACACAGCACAGTGTGTTGTAGTTGGTACTTAGTTCCTAACCCCATCCAATTTGGC |
| mm4_intergenic_Gm26685|Dpt_chr1_164783213 |
TGAGGTAAGATGCCAGAGTCCTTATGTCTTACCATCTCTTGGCATGAATTCATAGTCATGAAGTATTATGAGCGAATGCT TTAGAATGGGACGCACCACCTCTGTGTGGACTCAGGGTGAGGCAGAAAGCAGAGGA |
| mm4_intergenic_Gm6419|Rtdr1_chr10_74929333 |
AGCACTGCCATGTGGGATACAAGCAGGATTCTTTGCGTGTCTTCTGTGCCCCAGCACCTCTGTGTGGCAACACTGCATGC CTGTCCACACAACATTCTTGCCCC |
| mm4_intron_Gm15326_chr13_111870648 |
TGCTGTTCCTCCCACTGTTCCTATTTGAGCCTGTTCCTCCCTGTGGCGGTGCTTCACAGGCCTGTACAGAGTGTTGGGTG GC |
| mm4_intron_Fbxl7_chr15_26668685 |
GCATGCAGCATCAGCCAATGTGTGCCACATGGCCTCTGTGAGGCAGCGCATTGGCATTGGCCTCTCTGGTTTTGTCTATT CTGTCATCGTTTCCCATAGCTTTTCTGGGGCA |
| mm4_intron_Ank2_chr3_127363524 |
GTGTGGGGTTTAGGCTCTGGCTTCTTACCGCCCCCCCCCCCCCATCACCTTAGAAAGCCTTCTAAAACCCCCACCAGAGT TGGTGCGCCACAGTCCTCTTTGCTCATCGCTCTGGGTCACCCTTCCCCTTGTTTGG |
| mm4_intergenic_Cspg5|mmu-mir-6236_chr9_110263353 |
CAGCTGGGCTTACTGTCTGTTTATAGCCACCAAAGAGAGGCGGAGCGGCACAGCTGCTGCCTCTTGTATGTATGCAACTC TGCCCTAATTGCACACAATCTCAGGGAGGGGCTAGGGCATATA |
| mm4_intergenic_Gm10220|Gm7361_chr5_26206874 |
TTTGTGTCTGACTATCACCACTATTTCCTTATTTAAACAGTTTAAATGATTAGTATTCTGCTTTCCAAATATGGCGCTCC TCCTCTGGGAGGTGATAGCGGTGACCAATTATATGGTTGGCTAGTTGCATATAAAGG |
| mm4_intron_Afap1_chr5_35907365 |
TGTGAGAGGCTTCTGTTTGCAGTTTTAAGGTCAGGGGGGACTCCCAGCCCCACAGGGACTGTGCGGCACATTGGCCAAAG TATTGGTTTCAGAGTCAAAAGATCTTTATGTCCTGCTTCAGTAAGAGGGGATGGGAGGG |
| mm4_intron_Adck4_chr7_27255614 |
ATGCCCAGACAAGTCACAGGCAGGGGAACCTATAATAGAATTGATAGATCCGTCCCGGGCCACCTCCCAAAGGCAGTGTG GTACAGTGTGTCGGGGCTGGACAGGTTCCTTCCTCTCCCTGCTCTG |
| mm4_intergenic_Stk25|Bok_chr1_93666647 |
TTGCTTGTTCTCTCAGGGCCCCATGGCCATCAGATAGTGCTTTCCAGTCACCTATCTGCCTCACTGCCTCTTGGGGGCCC TGACTCCCCTGATTAATGATTTTAAAAGCTGCCTTTGACTCCTCC |
| mm4_exon_Vps37c_chr19_10714215 |
CCATGGTGCTTCCCAGACATCATCACTACATTGGGGTTGGTTTCTCTACACTCTCATTCTCAGAATGTAAAGGGTAGGGA CTGTGAGTGCAGCCCCGGCTCTGGGTGGGCTGGAGCCTGCTTAGTAATGA |
| mm4_intergenic_Prkar2b|Pik3cg_chr12_32061509 |
AATCCCACGCACCTTTCTCCCTGTCCCACGCAGAGTTGGTGCGGCGCAATTCCTGCTGAAACTTCAGCCTCCACCTTGGT GGCTTTTCCTTTTCCTAGAATCCAACCCCAAGAGAAACGTGCGTGGTG |
| mm4_intron_Cux2_chr5_121973231 |
GGACTTCCTTCCTCCTCCCTTGAGTTGTGGCTCCTCATGGGCTCACTGTGCCTCACGGCCTCTCTGGGGAATACCAGTTC CAAGCTGTGGGAAGATGTCCACA |
| mm4_intergenic_Gm11217|Gm23950_chr4_64280771 |
AGCAGGAGGGGAGTATTTGAAAGCAAAAAAGTAACCACCCAGAGGGAGTGGGGAACAAGTAGTAGTGAGATCATTGTGAG CAAAGCAAAATGAAATAGACATATGAAAATGTTATAATGAAACCAGTGCCACACAGT |
| mm4_intron_Clic5_chr17_44205453 |
ACATAACCACCTGGCGCGCGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNAAGTAACATGTCCTCCCACAGACAGTGTGGCACAAACCCCTTGCCCTCGCCCTTTG |
| mm4_intergenic_Gm17388|Htr1d_chr4_136408020 |
TACTCCGTGAAGCTGCCAAGAAGCAGAGAGCGTGGCCCTCCAGTGACCCCTCCCTGTGCCGCAGCAGCTCTTGGTGGTCC TTTGAATACAAGACTTCTTTTCCCGAGTGTACACTGGAAAGCATCGCACAGTTGG |
| mm4_exon_Pde11a_chr2_75991107 |
TGCGGTTCAACCAAAGCAAGTAGTTCAGGATGCGGAGGACCCTCCCGACCACAGGGTGGCGCAGCGGCTCTGGGTGGTTT ACAGTCTGTCTTCCCCGGC |
| mm3_exon_Lama5_chr2_180198981 |
CGCTGACAGAGAGGGAAGTGGAAATAGCCAAGGGCACAGTGGTCACACCTGTCCCCCTCAAAGCCGGTGCGGCACATGCA CTGGCCAGACTCTGGGTCACAGAGGCTGTTGGCTA |
| mm3_intergenic_Gm21957|4930533L02Rik_chr7_125255096 |
TGTCTGCCAAAAGCCTAAGGAAAGAAGGGTGTGTGCACAGCACCGCCTCTGGGAGAGGATGCCACATGCCTACAGAAAGA GAGCTTGGACTTGCCTCCCTCTG |
| mm4_exon_D230002A01Rik_chr8_91804755 |
CCTTTCCTCCCAAGGCTCAGAGTCCCCCAGAGGCGGTGCGGGCTGGCTATCCTTCAGCTTCTTGCAGAGGCTGCTGCGTA CGCTCCCCCCCCCCCCAGCTGGGGTTACAGGTTAATGAAACGCTCGTTTTCCTCAGTCA |
| mm4_exon_Ptpn12_chr5_20998598 |
TAGCTTCTGTTGAGGTCGGCTGGGTGTTGCTCTGGTGAGGCCATGTGCAGCACTGGCTTTGGGTGGTACCTGTCACTGTC CTGCCACACAGTGGTAACGGTTGGGAAGGCTGA |
| mm4_intron_Klc1_chr12_111785327 |
TGGATGCACGCTGAAGAGAGAGAGGAGTGCAAAGTAAGCTGTGCTGCACTGCCTGNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACTCACTCACCTGACTTCTCT |
| mm4_intergenic_Ccdc112|Gm24617_chr18_46334067 |
GGGCAGACATTTGAGCAGGATTTACAGCCTGCCAGACGTAGTGCGGCACAGAGAGATTTCCCCATCAAATCTCACAGCAT CATGGCTATTTCGGGATAACTCAATGCCAGC |
| mm4_exon_Fam65a_chr8_105622074 |
CCACTGGGGTTGGCAGAATGCCTGAAATGAAGTGCCTCCCAGCCGCGGTTCTGCACAGTCTTTGCCACACACAGTGTCGG AGGCCTGGCCATTTGGTCAGCTTGCCTTGCCTCATCACATCCCTTGG |