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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313932_F | TCGTCGGCAGCGTCGCTTGGCACTCTGGTTGGTA | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313932_R | GTCTCGTGGGCTCGGGCACCATCTTCTCTGGCGG | 60.8 | 1.00 |
| mm3_exon_Kcnh6_chr11_106034284_F | TCGTCGGCAGCGTCCCCTGGGAGTCCCTATAGTCA | 59.4 | 0.71 |
| mm3_exon_Kcnh6_chr11_106034284_R | GTCTCGTGGGCTCGGGCAAGACTCTCCCGAAGACC | 60.1 | 0.71 |
| mm4_intergenic_Gm14643|Gm14575_chrX_40524525_F | Primer3: not found at this Tm | N.d. | 0.69 |
| mm4_intergenic_Gm14643|Gm14575_chrX_40524525_R | Primer3: not found at this Tm | N.d. | 0.69 |
| mm4_intergenic_Tacc1|Gm10689_chr8_25411935_F | Primer3: not found at this Tm | N.d. | 0.54 |
| mm4_intergenic_Tacc1|Gm10689_chr8_25411935_R | Primer3: not found at this Tm | N.d. | 0.54 |
| mm3_intergenic_Ncoa2|Gm23169_chr1_13312797_F | TCGTCGGCAGCGTCTGTGCCAACCATTCAGAGCT | 59.8 | 0.54 |
| mm3_intergenic_Ncoa2|Gm23169_chr1_13312797_R | GTCTCGTGGGCTCGGGGCCAAGGCTGAGTCTACTG | 60.1 | 0.54 |
| mm4_intron_Cux1_chr5_136333304_F | Primer3: not found at this Tm | N.d. | 0.52 |
| mm4_intron_Cux1_chr5_136333304_R | Primer3: not found at this Tm | N.d. | 0.52 |
| mm4_intergenic_Usp9y|Zfy2_chrY_2091299_F | Primer3: not found at this Tm | N.d. | 0.48 |
| mm4_intergenic_Usp9y|Zfy2_chrY_2091299_R | Primer3: not found at this Tm | N.d. | 0.48 |
| mm4_intergenic_Ntf3|Kcna5_chr6_126372940_F | TCGTCGGCAGCGTCTGCCTGTGAGACCATAAGCG | 60.1 | 0.45 |
| mm4_intergenic_Ntf3|Kcna5_chr6_126372940_R | GTCTCGTGGGCTCGGTATTGGTGCTGGGCTGGATG | 60.1 | 0.45 |
| mm4_intergenic_Lilra6|Gm7363_chr7_3961321_F | TCGTCGGCAGCGTCTGAAACCCCTTCACCTGAAAA | 57.6 | 0.41 |
| mm4_intergenic_Lilra6|Gm7363_chr7_3961321_R | GTCTCGTGGGCTCGGACAATTTGTTCGGAACTTGTTGA | 57.6 | 0.41 |
| mm4_intergenic_Nrg3|Gm10713_chr14_39701302_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Nrg3|Gm10713_chr14_39701302_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Gm13044|Gm13041_chr4_143498733_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Gm13044|Gm13041_chr4_143498733_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Sdc3|Gm12970_chr4_130801862_F | TCGTCGGCAGCGTCACACTACCCCAACCAAGACC | 59.2 | 0.38 |
| mm4_intergenic_Sdc3|Gm12970_chr4_130801862_R | GTCTCGTGGGCTCGGCCCTCTGTGTTTAGCAGCTAG | 58.1 | 0.38 |
| mm4_intergenic_B3galtl|Rxfp2_chr5_149845413_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_B3galtl|Rxfp2_chr5_149845413_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intron_Zmat4_chr8_23668330_F | TCGTCGGCAGCGTCATGAGCTGCTCAGAGCCTTG | 60.1 | 0.37 |
| mm4_intron_Zmat4_chr8_23668330_R | GTCTCGTGGGCTCGGAGGAGAACCTCTGAAAGAAAAGTCT | 59.6 | 0.37 |
| mm3_intergenic_Gm10612/Cbfa2t3|Acsf3_chr8_122741589_F | TCGTCGGCAGCGTCGACCTTGGCCTTCTGTCTCC | 60.0 | 0.35 |
| mm3_intergenic_Gm10612/Cbfa2t3|Acsf3_chr8_122741589_R | GTCTCGTGGGCTCGGCTGGAGGCAGGTTAAGGTCC | 59.7 | 0.35 |
| mm4_intron_Habp2_chr19_56303538_F | TCGTCGGCAGCGTCGTCTGCCCATATGGAGAGGC | 59.9 | 0.31 |
| mm4_intron_Habp2_chr19_56303538_R | GTCTCGTGGGCTCGGGTCACCAGGGAAGGAGAAGC | 60.0 | 0.31 |
| mm4_intergenic_Baz2b|Gm13572_chr2_60012518_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Baz2b|Gm13572_chr2_60012518_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intron_Slc35d2_chr13_64103480_F | TCGTCGGCAGCGTCCTTGGGAAGCACCTGACACT | 59.8 | 0.30 |
| mm4_intron_Slc35d2_chr13_64103480_R | GTCTCGTGGGCTCGGCCACTTCTCCACAGCCTCAG | 60.0 | 0.30 |
| mm4_intergenic_Gm26297|Pvrl3_chr16_46307908_F | TCGTCGGCAGCGTCAGTTCAGTCCAACAGCAGGG | 59.8 | 0.26 |
| mm4_intergenic_Gm26297|Pvrl3_chr16_46307908_R | GTCTCGTGGGCTCGGAAGAGTGGAAGGGCTGGATG | 59.3 | 0.26 |
| mm4_intergenic_Tcf4|Gm24845_chr18_69531879_F | TCGTCGGCAGCGTCAGCAAAACCTTTCTGTATTACTCCA | 58.6 | 0.25 |
| mm4_intergenic_Tcf4|Gm24845_chr18_69531879_R | GTCTCGTGGGCTCGGCACTTTCCGACGGCCTAGAA | 59.7 | 0.25 |
| mm4_intergenic_Gm807|Zfp366_chr13_99118153_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm807|Zfp366_chr13_99118153_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_exon_Aif1l_chr2_31971316_F | TCGTCGGCAGCGTCTCCCATGTTTGAAGAGGGCC | 59.9 | 0.24 |
| mm4_exon_Aif1l_chr2_31971316_R | GTCTCGTGGGCTCGGCTCTGTGCCTGCTCTGGATC | 60.1 | 0.24 |
| mm4_intergenic_Gm5507|Redrum_chr18_54079804_F | TCGTCGGCAGCGTCGCGTTTATTAGAGGTACCATGCA | 58.8 | 0.23 |
| mm4_intergenic_Gm5507|Redrum_chr18_54079804_R | GTCTCGTGGGCTCGGCCTGCCTTCATCCAAGACGT | 60.0 | 0.23 |
| mm4_intergenic_Tec|Gm9870/Slain2_chr5_72895980_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Tec|Gm9870/Slain2_chr5_72895980_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_exon_Wnt7a_chr6_91364633_F | TCGTCGGCAGCGTCCCCTCCTTGACCCCTTCTCT | 60.2 | 0.21 |
| mm4_exon_Wnt7a_chr6_91364633_R | GTCTCGTGGGCTCGGTTGAGAAGCATGGGACCTGG | 59.6 | 0.21 |
| mm4_intergenic_A230072I06Rik|Gm22207_chr8_12309162_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_A230072I06Rik|Gm22207_chr8_12309162_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm3_intergenic_Gm23026|Exoc6_chr19_37528761_F | TCGTCGGCAGCGTCTTTGAGAACAATAAGGCAACTGCA | 59.6 | 0.20 |
| mm3_intergenic_Gm23026|Exoc6_chr19_37528761_R | GTCTCGTGGGCTCGGCTTGGCGGTTTTGCTGAGTC | 60.0 | 0.20 |
| mm4_intergenic_4921524J17Rik/1700051O22Rik|Gpt2_chr8_85470986_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_4921524J17Rik/1700051O22Rik|Gpt2_chr8_85470986_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_4931406P16Rik_chr7_34263776_F | TCGTCGGCAGCGTCGCCCCAACACCTGTGAAAAC | 59.8 | 0.20 |
| mm4_intron_4931406P16Rik_chr7_34263776_R | GTCTCGTGGGCTCGGTGCCAGTCTGTTGACAGTTGA | 59.7 | 0.20 |
| mm4_exon_Gm7580_chr7_74397236_F | TCGTCGGCAGCGTCGACACTGGCCACTCCTTTCA | 59.8 | 0.19 |
| mm4_exon_Gm7580_chr7_74397236_R | GTCTCGTGGGCTCGGGACTTCCATCACCCACCTGG | 60.0 | 0.19 |
| mm3_intron_Exoc2_chr13_30894084_F | TCGTCGGCAGCGTCAGGATTGTGCATGCAGAGCT | 60.0 | 0.19 |
| mm3_intron_Exoc2_chr13_30894084_R | GTCTCGTGGGCTCGGGAAGACTTGGAGGGGACACG | 60.0 | 0.19 |
| mm4_intergenic_Car4|Usp32_chr11_84980960_F | TCGTCGGCAGCGTCCAGGTCGTGGGCTGGTTATT | 60.0 | 0.18 |
| mm4_intergenic_Car4|Usp32_chr11_84980960_R | GTCTCGTGGGCTCGGACCAGCACAATGTTGTTGCT | 58.8 | 0.18 |
| mm4_intron_Pmp22_chr11_63145095_F | TCGTCGGCAGCGTCTTAAGGGAGCTGAGCAACCC | 59.6 | 0.18 |
| mm4_intron_Pmp22_chr11_63145095_R | GTCTCGTGGGCTCGGTGGGTGGACATCAGAGGACA | 60.1 | 0.18 |
| mm4_intergenic_Cep55|Ffar4_chr19_38094678_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Cep55|Ffar4_chr19_38094678_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm22181|Gm26994_chr8_112232067_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm22181|Gm26994_chr8_112232067_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_1700021A07Rik|1700020N01Rik_chr10_21486072_F | TCGTCGGCAGCGTCGGGTTTCATGGTCTTGGTTGC | 59.9 | 0.16 |
| mm4_intergenic_1700021A07Rik|1700020N01Rik_chr10_21486072_R | GTCTCGTGGGCTCGGAGGAGCTACGTTTGCAGCAT | 60.0 | 0.16 |
| mm4_intron_Tmem245_chr4_56930585_F | TCGTCGGCAGCGTCTGAGCTCAAGGACTGGGAGT | 60.1 | 0.16 |
| mm4_intron_Tmem245_chr4_56930585_R | GTCTCGTGGGCTCGGCCCATACTGACCCTGACCCT | 60.3 | 0.16 |
| mm4_intergenic_Pinx1|Sox7_chr14_63923103_F | TCGTCGGCAGCGTCTCCCTTGGGAAAGTGAACGT | 59.1 | 0.15 |
| mm4_intergenic_Pinx1|Sox7_chr14_63923103_R | GTCTCGTGGGCTCGGCACACTGGGAATGTGGGGAG | 60.3 | 0.15 |
| mm4_intron_Cdyl2_chr8_116588037_F | TCGTCGGCAGCGTCCCGCATCCTCTGATTCCCTG | 60.2 | 0.14 |
| mm4_intron_Cdyl2_chr8_116588037_R | GTCTCGTGGGCTCGGAGGGATGTTTACTCCACGCG | 60.1 | 0.14 |
| mm4_intron_Slc38a9_chr13_112719797_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Slc38a9_chr13_112719797_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intron_Ptprs_chr17_56467939_F | TCGTCGGCAGCGTCGACCCAGGTGCTGTTCTGAA | 59.8 | 0.14 |
| mm3_intron_Ptprs_chr17_56467939_R | GTCTCGTGGGCTCGGGAGGGAGTTGAGTGGTGCTC | 60.0 | 0.14 |
| mm4_intron_Clock_chr5_76303823_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Clock_chr5_76303823_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Impg1|RP24-325L16.1_chr9_80699486_F | TCGTCGGCAGCGTCCCCAGAACCCTATGATGCCC | 59.8 | 0.13 |
| mm4_intergenic_Impg1|RP24-325L16.1_chr9_80699486_R | GTCTCGTGGGCTCGGCTCTATGTGAGGGTCAGGTCC | 58.9 | 0.13 |
| mm4_intergenic_Gm13994|Slc24a5_chr2_124848803_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm13994|Slc24a5_chr2_124848803_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Bpifb5_chr2_154232858_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Bpifb5_chr2_154232858_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Smarcd3|1700022A21Rik_chr5_24623985_F | TCGTCGGCAGCGTCCAGGTGGGTAGAGGAAGGGA | 59.9 | 0.13 |
| mm4_intergenic_Smarcd3|1700022A21Rik_chr5_24623985_R | GTCTCGTGGGCTCGGCTCCTCCCTCTACCTGGCAT | 60.1 | 0.13 |
| mm4_intergenic_Gmnc|Ostn_chr16_27025650_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gmnc|Ostn_chr16_27025650_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Fblim1_chr4_141580930_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Fblim1_chr4_141580930_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Myo10_chr15_25758862_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Myo10_chr15_25758862_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Mast4_chr13_102854913_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Mast4_chr13_102854913_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm25781|Ipo5_chr14_120878351_F | TCGTCGGCAGCGTCATTGGGTGGGGCAGACAAAT | 59.8 | 0.12 |
| mm4_intergenic_Gm25781|Ipo5_chr14_120878351_R | GTCTCGTGGGCTCGGTCTCACAGGGACTCAGGGAG | 59.9 | 0.12 |
| mm4_intergenic_Gm11873|Nkain3_chr4_20458414_F | TCGTCGGCAGCGTCTCGACCATTGACAATATGCCT | 57.4 | 0.11 |
| mm4_intergenic_Gm11873|Nkain3_chr4_20458414_R | GTCTCGTGGGCTCGGGCTGACAGGGTTGCTCATGA | 60.3 | 0.11 |
| mm4_intergenic_Hsfy2|Satb2_chr1_56785922_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Hsfy2|Satb2_chr1_56785922_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Gm6851|Gm24910_chr7_40218493_F | TCGTCGGCAGCGTCCCCCAGTCTTCTCACTGACC | 59.3 | 0.10 |
| mm3_intergenic_Gm6851|Gm24910_chr7_40218493_R | GTCTCGTGGGCTCGGAGTGTGAGAGAGCTAGCGGT | 60.3 | 0.10 |
| mm4_intron_Ano1_chr7_144689014_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Ano1_chr7_144689014_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Chrng_chr1_87207580_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Chrng_chr1_87207580_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Aff3|Lonrf2_chr1_38700096_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Aff3|Lonrf2_chr1_38700096_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Gm26791_chr14_118375856_F | TCGTCGGCAGCGTCCACTACGGAACAAAGCTCGC | 59.5 | 0.10 |
| mm4_intron_Gm26791_chr14_118375856_R | GTCTCGTGGGCTCGGTGGTGGCTAGGCAGACTTTG | 59.9 | 0.10 |
| mm3_exon_Cryz_chr3_154611511_F | TCGTCGGCAGCGTCCTCTTGCAGCCGATGACACT | 60.3 | 0.10 |
| mm3_exon_Cryz_chr3_154611511_R | GTCTCGTGGGCTCGGAGGCTGTGAACTCAGAGTGC | 59.9 | 0.10 |
| mm4_intergenic_RP24-221A23.1|Gm7866_chr9_72168684_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_RP24-221A23.1|Gm7866_chr9_72168684_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_88820150_F | TCGTCGGCAGCGTCGGCAGGTAAGGCTTTCCTGT | 59.9 | 0.09 |
| mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_88820150_R | GTCTCGTGGGCTCGGACAGCCCAAAGATGAAGGCT | 59.5 | 0.09 |
| mm4_intron_Fam19a5_chr15_87721685_F | TCGTCGGCAGCGTCCACAGTTGGGTTTGAGGGGA | 59.8 | 0.09 |
| mm4_intron_Fam19a5_chr15_87721685_R | GTCTCGTGGGCTCGGCCAGGAGGCTACTATGTGGC | 59.6 | 0.09 |
| mm4_intron_Tmem132b_chr5_125589487_F | TCGTCGGCAGCGTCGATTTGGCCTTGAGGGTGGA | 59.9 | 0.09 |
| mm4_intron_Tmem132b_chr5_125589487_R | GTCTCGTGGGCTCGGTTCTCCTCCTACTCCTCGCC | 60.1 | 0.09 |
| mm4_exon_Polr1b_chr2_129113623_F | TCGTCGGCAGCGTCTTGCCAAGATGAGGACCACC | 59.9 | 0.08 |
| mm4_exon_Polr1b_chr2_129113623_R | GTCTCGTGGGCTCGGAGTGGTTCAACAGCCCACAA | 60.0 | 0.08 |
| mm4_intergenic_8430427H17Rik|Commd7_chr2_153557136_F | TCGTCGGCAGCGTCCCAGGCTTTCGGGAACTTCT | 59.9 | 0.08 |
| mm4_intergenic_8430427H17Rik|Commd7_chr2_153557136_R | GTCTCGTGGGCTCGGGGTGACAGTGACCCTTGGAG | 59.9 | 0.08 |
| mm4_intergenic_Edem1|Gm20387_chr6_109616400_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Edem1|Gm20387_chr6_109616400_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Nhsl2_chrX_102003403_F | TCGTCGGCAGCGTCTTATCCCTTGCTAGGCTGGG | 58.8 | 0.08 |
| mm4_intron_Nhsl2_chrX_102003403_R | GTCTCGTGGGCTCGGTACTGGGAACTGTGGGGACT | 59.8 | 0.08 |
| mm4_exon_Dysf_chr6_84186412_F | TCGTCGGCAGCGTCCAGAAGATCCAGCCATCCCC | 59.8 | 0.07 |
| mm4_exon_Dysf_chr6_84186412_R | GTCTCGTGGGCTCGGTTACCTTGCCATTGGGGTCC | 59.9 | 0.07 |
| mm3_exon_Abca7_chr10_80015112_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_exon_Abca7_chr10_80015112_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Vwc2_chr11_11197230_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Vwc2_chr11_11197230_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_1700001G01Rik|Gm26235_chr18_17926538_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_1700001G01Rik|Gm26235_chr18_17926538_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Gm12257|Btnl10_chr11_58906489_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Gm12257|Btnl10_chr11_58906489_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm12286|B130011K05Rik_chr11_63340047_F | TCGTCGGCAGCGTCACCAACTCAGTTTCCGTGCA | 60.1 | 0.07 |
| mm4_intergenic_Gm12286|B130011K05Rik_chr11_63340047_R | GTCTCGTGGGCTCGGTGTAAACACTCACCAAATGGGA | 57.8 | 0.07 |
| mm4_intron_Cntnap5a_chr1_116247148_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Cntnap5a_chr1_116247148_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm23752|Gm24815_chr12_7536663_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm23752|Gm24815_chr12_7536663_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm12012|Sec61g_chr11_16441543_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm12012|Sec61g_chr11_16441543_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_4930505O20Rik|Frem3_chr8_80591399_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_4930505O20Rik|Frem3_chr8_80591399_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Trappc10_chr10_78221446_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Trappc10_chr10_78221446_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Ndel1|Rnf222_chr11_68879790_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Ndel1|Rnf222_chr11_68879790_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Slc30a10|Lyplal1_chr1_185945229_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Slc30a10|Lyplal1_chr1_185945229_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Npsr1_chr9_24229908_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Npsr1_chr9_24229908_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm23117|Gm5083_chr13_43961285_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm23117|Gm5083_chr13_43961285_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_n-R5s14|Hsd17b10_chrX_151987894_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_n-R5s14|Hsd17b10_chrX_151987894_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Eln|Wbscr28_chr5_134797996_F | TCGTCGGCAGCGTCAAAGAAAGACGTCCGGCCAG | 60.6 | 0.06 |
| mm4_intergenic_Eln|Wbscr28_chr5_134797996_R | GTCTCGTGGGCTCGGGGCCCTAGACGTCTGTCCTA | 60.1 | 0.06 |
| mm4_intergenic_Gm25571|Gm21969/Iffo2_chr4_139589594_F | TCGTCGGCAGCGTCTTTCTGCTGGGGTGACAGAC | 59.8 | 0.06 |
| mm4_intergenic_Gm25571|Gm21969/Iffo2_chr4_139589594_R | GTCTCGTGGGCTCGGACCAAACATCAAAGGAGTGCG | 59.3 | 0.06 |
| mm4_intron_Anks6_chr4_47030372_F | TCGTCGGCAGCGTCCTAAGGCGCAGACCTAGAGC | 59.9 | 0.05 |
| mm4_intron_Anks6_chr4_47030372_R | GTCTCGTGGGCTCGGTTGTGGTCCCTGCTGTTCTC | 59.8 | 0.05 |
| mm4_intron_Pde9a_chr17_31438343_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Pde9a_chr17_31438343_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Nxn_chr11_76377455_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Nxn_chr11_76377455_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Ccnd1|Tpcn2_chr7_145102425_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Ccnd1|Tpcn2_chr7_145102425_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_exon_Igkv15-103_chr6_68437858_F | TCGTCGGCAGCGTCGTGGCAGTGGATCTGGAACA | 59.9 | 0.05 |
| mm3_exon_Igkv15-103_chr6_68437858_R | GTCTCGTGGGCTCGGCCCTGGGAGGTTTGTGTCAT | 59.5 | 0.05 |
| mm4_intergenic_Gm9824|Gm9996/Soga3_chr10_29070641_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm9824|Gm9996/Soga3_chr10_29070641_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Nox3|Gm23186_chr17_3698277_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Nox3|Gm23186_chr17_3698277_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm15083|Slc6a11_chr6_114114671_F | TCGTCGGCAGCGTCCATCTTCCCCACTCCAGCTG | 60.1 | 0.04 |
| mm4_intergenic_Gm15083|Slc6a11_chr6_114114671_R | GTCTCGTGGGCTCGGAAGCCATCACCCAAGGAAGG | 59.9 | 0.04 |
| mm4_intergenic_Gm13492|Gm13493_chr2_51828428_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm13492|Gm13493_chr2_51828428_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intron_Lgr6_chr1_135030875_F | TCGTCGGCAGCGTCATGCTTCCACGTGCACACTA | 59.9 | 0.04 |
| mm3_intron_Lgr6_chr1_135030875_R | GTCTCGTGGGCTCGGGTTTCAGGGGCCCTACACAA | 59.8 | 0.04 |
| mm4_exon_Ssc5d_chr7_4942756_F | TCGTCGGCAGCGTCAGTCTTCCTGGACGTGGGAT | 60.2 | 0.04 |
| mm4_exon_Ssc5d_chr7_4942756_R | GTCTCGTGGGCTCGGCTGTGGATCCAGGCTTGGTT | 59.9 | 0.04 |
| mm4_intergenic_Gm8730|Cdh5_chr8_104068649_F | TCGTCGGCAGCGTCTCGGAACCAGAATCCCTCCT | 59.9 | 0.03 |
| mm4_intergenic_Gm8730|Cdh5_chr8_104068649_R | GTCTCGTGGGCTCGGTTCGGAAGGAGGTGCAACTC | 59.9 | 0.03 |
| mm4_intergenic_Gm25661|Zfp868_chr8_69538520_F | TCGTCGGCAGCGTCACTTCGGCACATTCCTCCTC | 59.7 | 0.03 |
| mm4_intergenic_Gm25661|Zfp868_chr8_69538520_R | GTCTCGTGGGCTCGGGGTGCAACTCAAACTTCCGG | 59.6 | 0.03 |
| mm4_intergenic_Gm25064|Skor1_chr9_63137115_F | TCGTCGGCAGCGTCTCGACTTCGCTTCCAAGGTC | 60.0 | 0.03 |
| mm4_intergenic_Gm25064|Skor1_chr9_63137115_R | GTCTCGTGGGCTCGGGAGGCTCAGGGAAACACTCC | 60.0 | 0.03 |
| mm4_intron_Mkl1_chr15_81083199_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Mkl1_chr15_81083199_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Crtac1_chr19_42334253_F | TCGTCGGCAGCGTCAGCCTTGCCTCAGGAAACTC | 59.9 | 0.02 |
| mm4_intron_Crtac1_chr19_42334253_R | GTCTCGTGGGCTCGGCAGCCAGGCCCTTAGTTAGG | 59.8 | 0.02 |
| mm4_exon_Herc2_chr7_56153316_F | TCGTCGGCAGCGTCTCCACGTTGAGAAAGCGGAG | 60.3 | 0.02 |
| mm4_exon_Herc2_chr7_56153316_R | GTCTCGTGGGCTCGGATTCCACTGGGTCAGGGAGT | 60.1 | 0.02 |
| mm4_intergenic_Pon2|Asb4_chr6_5306002_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Pon2|Asb4_chr6_5306002_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Card10|Cdc42ep1_chr15_78825170_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Card10|Cdc42ep1_chr15_78825170_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_1110038F14Rik|Mb_chr15_77009880_F | TCGTCGGCAGCGTCCACTTGGCTCCAGAGGAAGG | 60.0 | 0.01 |
| mm4_intergenic_1110038F14Rik|Mb_chr15_77009880_R | GTCTCGTGGGCTCGGCTTGCCATTGCCACCAGAAC | 60.0 | 0.01 |
| mm3_intron_1700021F05Rik_chr10_43530880_F | TCGTCGGCAGCGTCTCTGAAAAGGCTCGCTGCTT | 60.2 | 0.00 |
| mm3_intron_1700021F05Rik_chr10_43530880_R | GTCTCGTGGGCTCGGCCATATGTGTGGTGCCTGGT | 60.0 | 0.00 |
| mm4_intron_Zfp407_chr18_84379767_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zfp407_chr18_84379767_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Trim8_chr19_46503821_F | TCGTCGGCAGCGTCTAAGGTTGAGCCGCGTTGAA | 60.2 | 0.00 |
| mm4_intron_Trim8_chr19_46503821_R | GTCTCGTGGGCTCGGAAGGCGGTTTTGACATTGGC | 59.9 | 0.00 |
| mm4_intron_Rph3al_chr11_75916597_F | TCGTCGGCAGCGTCCGTGCTGGGAGAGACAAGTT | 59.9 | 0.00 |
| mm4_intron_Rph3al_chr11_75916597_R | GTCTCGTGGGCTCGGATAAGCCTCTCCTGCCCCTG | 61.0 | 0.00 |
| mm4_intron_Myo9b_chr8_71356673_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Myo9b_chr8_71356673_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Mtrf1_chr14_79406725_F | TCGTCGGCAGCGTCTTACCCTGAGTTGACGTCGC | 60.0 | 0.00 |
| mm4_intron_Mtrf1_chr14_79406725_R | GTCTCGTGGGCTCGGATGAGCACCATCCTTAGGCG | 59.8 | 0.00 |
| mm4_intron_Erc2_chr14_28043468_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Erc2_chr14_28043468_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Armc2_chr10_42005618_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Armc2_chr10_42005618_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Akap6_chr12_52916363_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Akap6_chr12_52916363_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Tab2|Ust_chr10_8117589_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Tab2|Ust_chr10_8117589_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Sdc1|9930038B18Rik_chr12_8823459_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Sdc1|9930038B18Rik_chr12_8823459_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rpp25l|Dctn3_chr4_41713633_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rpp25l|Dctn3_chr4_41713633_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pcbd2|4930451E10Rik/Pcbd2_chr13_55757358_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pcbd2|4930451E10Rik/Pcbd2_chr13_55757358_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25132|Dclk1_chr3_55358639_F | TCGTCGGCAGCGTCGTGTGCTGGCCCTACAGTAG | 60.1 | 0.00 |
| mm4_intergenic_Gm25132|Dclk1_chr3_55358639_R | GTCTCGTGGGCTCGGCAAGTGGGTGGCACTTTGC | 59.9 | 0.00 |
| mm4_intergenic_Gm23022|Rab28_chr5_41161815_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23022|Rab28_chr5_41161815_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm10549|2410004N09Rik_chr18_33783020_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm10549|2410004N09Rik_chr18_33783020_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cbx8|Cbx4/Gm26508_chr11_119074689_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cbx8|Cbx4/Gm26508_chr11_119074689_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Muc5b_chr7_141856061_F | TCGTCGGCAGCGTCTCATGGGGAATGTGAGTGGC | 60.0 | 0.00 |
| mm4_exon_Muc5b_chr7_141856061_R | GTCTCGTGGGCTCGGAAGGTTCCAGCCTCACCTTC | 59.5 | 0.00 |
| mm3_intron_Gm4409_chr6_79973440_F | TCGTCGGCAGCGTCGCAGACTGCTAAGCTTAGATACA | 58.0 | 0.00 |
| mm3_intron_Gm4409_chr6_79973440_R | GTCTCGTGGGCTCGGACAGCTGTGCTTGTGTATATGC | 59.3 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313932 |
GCTTGGCACTCTGGTTGGTAAGAGGGTCTCTAATTTGTCCGAAGCCGTGAGCAGCCCGCTGCCTGAACCGCCAGAGAAGA TGGTGC |
| mm3_exon_Kcnh6_chr11_106034284 |
CCCTGGGAGTCCCTATAGTCAGCAAGCCAGGCAGAGGGCTGCTCAGGGGTAGCTCACAGGATGTAAGAGTCAGTGGAGAA GAGAGATGCTTCGGGTCTTCGGGAGAGTCTTGC |
| mm3_intergenic_Ncoa2|Gm23169_chr1_13312797 |
TGTGCCAACCATTCAGAGCTATACTTAAAGGCCCTGAGCTGCCCACTGTCTGAAGAACCACAGTAGACTCAGCCTTGGCC |
| mm4_intergenic_Ntf3|Kcna5_chr6_126372940 |
TGCCTGTGAGACCATAAGCGGACACATATTGGTTTGGGTTTGCACCTTGAGTTGCCCACTGCCTGCACAGTGAGGCTATA TGGTTTCCTGTGCCCCATCCAGCCCAGCACCAATA |
| mm4_intergenic_Lilra6|Gm7363_chr7_3961321 |
TGAAACCCCTTCACCTGAAAAAATGACAACAACAAAAATCCCAAAAACATTACAATACAAAACAAAACAAAATAATGGAA ATCTTCAGGTACTAGGCTGCTCATGGCTATATATCTATCAACAAGTTCCGAACAAATTGT |
| mm4_intergenic_Sdc3|Gm12970_chr4_130801862 |
ACACTACCCCAACCAAGACCCCTTGAGCAGCCCATTGACTGACCTTTTTCACACCTATGTAGAATCCTCCAGTGNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAGCTGCTAAACACAGAGGG |
| mm4_intron_Zmat4_chr8_23668330 |
ATGAGCTGCTCAGAGCCTTGCACACAGCGGGCAGGTATTGCTAAGCTCTGCCCATGAGCTGCCCTCTGCCTTGAAACTAG ATTTTCTAAGACTTTTCTTTCAGAGGTTCTCCT |
| mm3_intergenic_Gm10612/Cbfa2t3|Acsf3_chr8_122741589 |
GACCTTGGCCTTCTGTCTCCCCTGAAAGCTCAAGACCCTGGGCAGCCCTCTGCCTGACCCTTCCTGATGGACCTTAACCT GCCTCCAG |
| mm4_intron_Habp2_chr19_56303538 | GTCTGCCCATATGGAGAGGCCTTGATAACGTCCTTGTGCAGCCCTCTAACTGAATGGCTTCTCCTTCCCTGGTGAC |
| mm4_intron_Slc35d2_chr13_64103480 |
CTTGGGAAGCACCTGACACTACCCCTGAGCTGCCTGCTGAGTGAACAGCAGGAGCTGGTGGCCAGCCATCCTGAAGAGTG ACTTCTGAGGCTGTGGAGAAGTGG |
| mm4_intergenic_Gm26297|Pvrl3_chr16_46307908 |
AGTTCAGTCCAACAGCAGGGTTAATACTAATTGATGAGATCTGCTTTTAACCATCATAGAAGATTGCCTTTCCCTGTGCA GGCCACTGTCTGAACTGAACATTTCCGTTTCTTGGCATCCAGCCCTTCCACTCTT |
| mm4_intergenic_Tcf4|Gm24845_chr18_69531879 |
AGCAAAACCTTTCTGTATTACTCCAGATGATTGTAAGGGAACGGGCTGCTCAAGGGGGTGGTATATTCTTTCTCCAAGGA GAGCATAGGCTCAGGGGATAGATTCTTCTAGGCCGTCGGAAAGTG |
| mm4_exon_Aif1l_chr2_31971316 |
TCCCATGTTTGAAGAGGGCCTGTCCGTCAGTTCATCTTTCTCAGCTTCTAAATTAAATTCAGGCTGCAAGCTGCTCTCGG AAGATCCAGAGCAGGCACAGAG |
| mm4_intergenic_Gm5507|Redrum_chr18_54079804 |
GCGTTTATTAGAGGTACCATGCATAATGATGAGGAGAATTCATAAAACCCCGAACAGCCCACTGCCTAAAGGAAGAAACA GTAACGTCTTGGATGAAGGCAGG |
| mm4_exon_Wnt7a_chr6_91364633 |
CCCTCCTTGACCCCTTCTCTTAGGACTACACCTCACCTAGAGGCAGTGCCTGGGCTGTCCTGATGGGATAGCGGCATCCC TGAGCTGGCCTCTGCCTGATGATGAATGCCAGGTCCCATGCTTCTCAA |
| mm3_intergenic_Gm23026|Exoc6_chr19_37528761 |
TTTGAGAACAATAAGGCAACTGCAGGTTATTAATATGAAAATAGTGTTGATTGTTTCTTCTGAAGAAATCTCCCTGGGCA GCCCGTTTCCTGAAAAATGACTCAGCAAAACCGCCAAG |
| mm4_intron_4931406P16Rik_chr7_34263776 |
GCCCCAACACCTGTGAAAACAAAAGAGCAAGGGGATTTTAGGCTGTGGGTTGCTCATGGATTAGTGCAGTAACTCCTCAA CTGTCAACAGACTGGCA |
| mm4_exon_Gm7580_chr7_74397236 |
GACACTGGCCACTCCTTTCATCAACTCTTAGCACATGCCTCTGAGGCATAGGGCTGCTCATGGCTACCACCACTGTGGAG AGCTGGATCGAACTCCAGCCTGCCAGGTGGGTGATGGAAGTC |
| mm3_intron_Exoc2_chr13_30894084 |
AGGATTGTGCATGCAGAGCTGTGAGGCACTTTGAGCAGCTCACTGCCTGCAGCCCACTCACCACTGTGAACAGAGAAAAA GCCAGTCAGGTGTGACGTGTCCCCTCCAAGTCTTC |
| mm4_intergenic_Car4|Usp32_chr11_84980960 |
CAGGTCGTGGGCTGGTTATTTACAGGTGCCCTGTCCCTAGGACAGCTGCCAGGCAGCAGGCTGCTCCAGGCACTACCTGC ATCTCCACAGTAAGAGCTTTATCTGGCCTTAAGCAACAACATTGTGCTGGT |
| mm4_intron_Pmp22_chr11_63145095 |
TTAAGGGAGCTGAGCAACCCCCTTAGGGCACTGCAGGCTTCTCCTTGAGCAGCCAGCTAGCTGAGCCAGTCTTGGGACTG TCATGGAGGATGCTTTCCCCAAGCTTGGACTCTGCTTGTCCTCTGATGTCCACCCA |
| mm4_intergenic_1700021A07Rik|1700020N01Rik_chr10_21486072 |
GGGTTTCATGGTCTTGGTTGCAGTTAAATGGAAAGACTGATTAAGCAAGACCGTGATTATCCCGCTGCCTGATGGGAGCA AATGCCAGGCATGCTGCAAACGTAGCTCCT |
| mm4_intron_Tmem245_chr4_56930585 |
TGAGCTCAAGGACTGGGAGTGGGGGGTGGCGTGGAGAACACCCAAGGTTTCAGGTAGCAGTCTGCACAGGGTCAGGGTCA GGGTCAGTATGGG |
| mm4_intergenic_Pinx1|Sox7_chr14_63923103 |
TCCCTTGGGAAAGTGAACGTAGATGCCAAAAAACAGTTATTGTAAAAACATGTCTTGATCACCAACTTTAGGCTTCAGGC TGCTCACGGTAGAAGAGCTGACCCATGAAGATCTCCCCACATTCCCAGTGTG |
| mm4_intron_Cdyl2_chr8_116588037 |
CCGCATCCTCTGATTCCCTGAGTGACCGCACGGAAGTCAGCCTCTGCTGGAACCATCCACAGGACTGAGAGACCCTGAGC AGTCGGCAGCCTGAGGCTCCGAGACTCCGCGTGGAGTAAACATCCCT |
| mm3_intron_Ptprs_chr17_56467939 |
GACCCAGGTGCTGTTCTGAATTCAGGCTGAGTGCTGCTCAAGGCACTTCCACTTTGCAGCCTCCGCCCTGGCCTCTGAAG TGACCCCTGTCCCACACACCACCATCCACCCTCCCCAGGAGCACCACTCAACTCCCTC |
| mm4_intergenic_Impg1|RP24-325L16.1_chr9_80699486 |
CCCAGAACCCTATGATGCCCACTGGATAGTAGATAAAGAGAGACTGTGAGCAATGCTCTACTCTGCACATCTGGAGAGAT GCCACCTTGATCTGCCCAGTGCCTGAAGAGTCTCCTCAGGGACCTGACCCTCACATAGAG |
| mm4_intergenic_Smarcd3|1700022A21Rik_chr5_24623985 |
CAGGTGGGTAGAGGAAGGGATGCCAGTCAAGGGGAGGAGGGATGACAGGTAAAGGGAAGGATGCCAGGAAGACGAAGGAT CAAGGATGCCAGGCAGCGGGATGATCAAGGATGCCAGGTAGAGGGAGGAG |
| mm4_intergenic_Gm25781|Ipo5_chr14_120878351 | ATTGGGTGGGGCAGACAAATGCAAGATCCCTGAGCAGCCCACACCCAGAAGGCTAACTCCTCCCTGAGTCCCTGTGAGA |
| mm4_intergenic_Gm11873|Nkain3_chr4_20458414 |
TCGACCATTGACAATATGCCTTGAGAATGAATTCATTGAGGCCCCAGGCTGCTCAAGGGGACTATGTATGTGAGGATTTA ACCGAAGACTCTCATGAGCAACCCTGTCAGC |
| mm3_intergenic_Gm6851|Gm24910_chr7_40218493 |
CCCCAGTCTTCTCACTGACCTAATGGCAATTTACATAAGCCCTCAGCAGCCTTCTGCCTGAAATTCTACCGCTAGCTCTC TCACACT |
| mm4_intron_Gm26791_chr14_118375856 |
CACTACGGAACAAAGCTCGCCAGGCCATGAAGTACGGGATCCCCAGGGCAGCCTCCTGCCTGAAGCCCAGCATCTCTGGC AGCAGGTAGATAATGTGATCTGCTGCGAGAGCAAAGTCTGCCTAGCCACCA |
| mm3_exon_Cryz_chr3_154611511 |
CTCTTGCAGCCGATGACACTATCTACCCCCTGCCCGAGACACTGAACTTCAGGCAGGGGGCTGCCCTGGGGATCCCGTAC TTCACGGCCTGCCGAGCTCTGTTCCACAGGTGAGGTGGCACTCTGAGTTCACAGCCT |
| mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_88820150 |
GGCAGGTAAGGCTTTCCTGTCCTTCCTTGGAGAATATGTGGTGTTAATTAAATGCAGCCGGCTGCTCATGGAAGTGTTTC TAATGCCAAGTAGCCTTCATCTTTGGGCTGT |
| mm4_intron_Fam19a5_chr15_87721685 |
CACAGTTGGGTTTGAGGGGAGTAATAGACAATGATGAGCCTCATCACCCATAACCAGGCAGAGGGCTGCTGAAGGCATGT CCCATTGCCACATAGTAGCCTCCTGG |
| mm4_intron_Tmem132b_chr5_125589487 |
GATTTGGCCTTGAGGGTGGAGAGGGGAAAAGAAAGTGTCTCCATGGGCAGCCAGCCTCCTGAAGGGAAGGCTCCAGCCTT CCAGCCCCTGGAAAAGTGAGACTGCTGGGAGAGGCGAGGAGTAGGAGGAGAA |
| mm4_exon_Polr1b_chr2_129113623 |
TTGCCAAGATGAGGACCACCACCGTGCGCAGGCTGCTGCCAGAATCCTGGGGCTTCCTCTGCCCTGTGCACACTCCAGAC GGGGCGCCTTGTGGGCTGTTGAACCACT |
| mm4_intergenic_8430427H17Rik|Commd7_chr2_153557136 |
CCAGGCTTTCGGGAACTTCTCCAATTTCTGCAGTTCCAGCCACATCTAGAAGGAACCCAGGGCAGCCCCCTGCCTGGACT CTGATCCAACAGAGGCCACAAGCACTCCAAGGGTCACTGTCACC |
| mm4_intron_Nhsl2_chrX_102003403 |
TTATCCCTTGCTAGGCTGGGACAGCTGGTCAGTAGTCTGAGGGATGTTTGTGGATTTGTTAAAGACTGAAGAGACCCTTA GCAGCTGGCTCCCTGAAGCTTGCAGTCCCCACAGTTCCCAGTA |
| mm4_exon_Dysf_chr6_84186412 |
CAGAAGATCCAGCCATCCCCATGCCCCCGAGACAATTCCATCAGCTGGCTGCTCAGGGTCCCCAGGAATGCTTGGTCCGT ATTTATATCGTCCGAGCATTTGGCTTACAGCCCAAGGACCCCAATGGCAAGGTAA |
| mm4_intergenic_Gm12286|B130011K05Rik_chr11_63340047 |
ACCAACTCAGTTTCCGTGCATCCTGGAGCAGCCTCCTGCCTGAGGCTGTTAGAGTCTAAGTCATTTAAACAGCTTGATTA ATTTGAATTTAATCCCATTTGGTGAGTGTTTACA |
| mm4_intergenic_Eln|Wbscr28_chr5_134797996 |
AAAGAAAGACGTCCGGCCAGGCCAGAGCTCAGGGCCTGCTTGGCTCGGGGGCAGCTGAGGGGACGTCAGGCGGGGGGCTG CTCCGGGCTTGGCTAGGACAGACGTCTAGGGCC |
| mm4_intergenic_Gm25571|Gm21969/Iffo2_chr4_139589594 | TTTCTGCTGGGGTGACAGACAGCTGTCACCCTTAGCAGGCAGCTGCCTTAACTTATCGCACTCCTTTGATGTTTGGT |
| mm4_intron_Anks6_chr4_47030372 |
CTAAGGCGCAGACCTAGAGCTGAGGACCGTGAGAGGTAGCTCAGTGAGCTGTGGGTTAATGCAGCAGGCTGCTCCTGGCC CTGGGGTTAACCCTCCACTGTCAACAGAGAACAGCAGGGACCACAA |
| mm3_exon_Igkv15-103_chr6_68437858 |
GTGGCAGTGGATCTGGAACAGGTTTCACATTAACCATCAGCAGCCTGCAGCCTGAAGACATTGCCACTTACTACTGTCAA CAGGGTCAAAGTTATCCTCTCACAGTGATATAAGTCATGACACAAACCTCCCAGGG |
| mm4_intergenic_Gm15083|Slc6a11_chr6_114114671 |
CATCTTCCCCACTCCAGCTGGAGGTGACTCACCTACAGACCTGAGTAGCAAAGGTTACCCCCTGGAGCAAGATCCAGGCA GGGTGATGCTCAGGGCATAGAGCATCCTTCCTTGGGTGATGGCTT |
| mm3_intron_Lgr6_chr1_135030875 |
ATGCTTCCACGTGCACACTACAGTCCTGAGCAGCCCACTTCCTGCATTTTGTGCAAACCATAGGCACGAGAGCTTGTGTA GGGCCCCTGAAAC |
| mm4_exon_Ssc5d_chr7_4942756 |
AGTCTTCCTGGACGTGGGATACACCTTCAGGAAGGGGTCTGTTCAAGGGGACCCCCACCACAACCAAGCCTGGATCCACA G |
| mm4_intergenic_Gm8730|Cdh5_chr8_104068649 |
TCGGAACCAGAATCCCTCCTCTACCCCTCTCTCCGTGACCAGCCTGCTGCCAGGAGACAGTGTTAAGCTATCAGCTACAG AAAACTTCCGGAAGTTTGAGTTGCACCTCCTTCCGAA |
| mm4_intergenic_Gm25661|Zfp868_chr8_69538520 |
ACTTCGGCACATTCCTCCTCCTCCCCCCCCCACCCCCCCCCCGTGACCAGCCTGCTGCCAGGAGACAGTGTTAAGCTATC AGCTACAGAAAACTTCCGGAAGTTTGAGTTGCACC |
| mm4_intergenic_Gm25064|Skor1_chr9_63137115 |
TCGACTTCGCTTCCAAGGTCCCCTAGAGAGTGCTTGGGGAGAGCCTGCGGTGCTAGGCTGAGAGCCTGTTCTTGGAGGCG ATGGTCCAGGCAGCGGCCTGCAGATGGGCTTTCTTCTTGGAGTGTTTCCCTGAGCCTC |
| mm4_intron_Crtac1_chr19_42334253 |
AGCCTTGCCTCAGGAAACTCAGAAAGAAAGGAAGAAACCGAGGCTAGAAAAAAGTGGATCAGAGTCATGTAGAACACAAT TCAAGGAGTGGGCTGGTCAAGGAGAGTGGGCAAGCCCTAACTAAGGGCCTGGCTG |
| mm4_exon_Herc2_chr7_56153316 |
TCCACGTTGAGAAAGCGGAGGGCCCGGCCCCAGGCTTCCTTGACTGCCACCCACAGCAGCACGCTGGCTGAAGAAGTGGT GGGGCTGCTCCGCACTCTGCACTCCCTGACCCAGTGGAAT |
| mm4_intergenic_1110038F14Rik|Mb_chr15_77009880 |
CACTTGGCTCCAGAGGAAGGCAGAGTGGGAGAAGCCAGCTCCTGCCACCACCGTGACCAGCAGGCTGTCTGAACTATGTT CTGGTGGCAATGGCAAG |
| mm3_intron_1700021F05Rik_chr10_43530880 |
TCTGAAAAGGCTCGCTGCTTCTCAGGCAGGTGGCTGCTCATGAGTCACAATGGTCAGAATGAAAGGGGGGTTTCCTGCCC CTCAGCTACCTGCTTGGCACTCAAGAGTGCAAAACCAGGCACCACACATATGG |
| mm4_intron_Trim8_chr19_46503821 |
TAAGGTTGAGCCGCGTTGAAATCTGGTTTCAGGCAGCTGGCTCCCCTGGGCTGCCGGCTGGGTGATTACGAACCGCACCC CCTCCCATTGTATACACCCTACTGCAGCAGCCAATGTCAAAACCGCCTT |
| mm4_intron_Rph3al_chr11_75916597 |
CGTGCTGGGAGAGACAAGTTGAGCAGAGGCTGCGTCTAGTAGACACACTGAGGGGCCCTTCAGGCTGTGGGGTGCTGAAG GCAGGCAGGGGCAGGAGAGGCTTAT |
| mm4_intron_Mtrf1_chr14_79406725 |
TTACCCTGAGTTGACGTCGCTGTCCCTTGAGAAGCCCGCTGCGTGTGTTTATTTTAGGACATGCCCATTTCCTGGGTGCT CACGCCTAAGGATGGTGCTCAT |
| mm4_intergenic_Gm25132|Dclk1_chr3_55358639 |
GTGTGCTGGCCCTACAGTAGGGAGGGCTCCCGAGGAGGAAATGCCTCACCCTGAAGAGCCTGCTGCCTGTATTCCCATGA CTGCCATGGCAAAGTGCCACCCACTTG |
| mm4_exon_Muc5b_chr7_141856061 |
TCATGGGGAATGTGAGTGGCACTATCAGCCCTGTGGAGCCCCCTGTCTGAAGACCTGTCGGAACCCAACTGGACACTGCC TCGTTGACCTGCCAGGTCTAGAAGGTGAGGCTGGAACCTT |
| mm3_intron_Gm4409_chr6_79973440 |
GCAGACTGCTAAGCTTAGATACAATGAGCATCAGTAAGGATATACATGTGATGTCCTTCCCCTGAGAAGCCCCTTGCCTG AAAAAAAAGCATATACACAAGCACAGCTGT |