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Contents:

PCR primers for off-targets of GACATGGTTATGTTTCTGGA TGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313859_F Primer3: not found at this Tm N.d. 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313859_R Primer3: not found at this Tm N.d. 1.00
mm3_intergenic_Cd274|Pdcd1lg2_chr19_29400401_F TCGTCGGCAGCGTCACCCTTGTAAGTGGATCGAGTC 59.5 0.70
mm3_intergenic_Cd274|Pdcd1lg2_chr19_29400401_R GTCTCGTGGGCTCGGGCTGAAAGCTTTGGATCAGG 57.0 0.70
mm4_intron_Angpt2_chr8_18697142_F Primer3: not found at this Tm N.d. 0.69
mm4_intron_Angpt2_chr8_18697142_R Primer3: not found at this Tm N.d. 0.69
mm4_intergenic_Olig3|Ifngr1_chr10_19397372_F Primer3: not found at this Tm N.d. 0.65
mm4_intergenic_Olig3|Ifngr1_chr10_19397372_R Primer3: not found at this Tm N.d. 0.65
mm4_intergenic_Gm8349|Arhgef26_chr3_61823664_F Primer3: not found at this Tm N.d. 0.60
mm4_intergenic_Gm8349|Arhgef26_chr3_61823664_R Primer3: not found at this Tm N.d. 0.60
mm4_intron_Rnf152_chr1_105304855_F TCGTCGGCAGCGTCACCACTTCTGCAATCCCAGT 59.2 0.57
mm4_intron_Rnf152_chr1_105304855_R GTCTCGTGGGCTCGGACTAATGGTCCAAGGAAGGGG 59.0 0.57
mm4_intergenic_Sphkap|Gm25754_chr1_83586993_F TCGTCGGCAGCGTCCAGTGGTTCCAGGAAGTCCA 59.2 0.57
mm4_intergenic_Sphkap|Gm25754_chr1_83586993_R GTCTCGTGGGCTCGGAGGCTCCACAAAGACAAGGAG 59.9 0.57
mm4_intron_Bcor_chrX_12096525_F TCGTCGGCAGCGTCTTTCTTGGTCAGAGGAGCCG 59.6 0.54
mm4_intron_Bcor_chrX_12096525_R GTCTCGTGGGCTCGGCTAAGGGCTCCTGCTGTCAC 60.1 0.54
mm3_intron_Arhgap15_chr2_44384698_F TCGTCGGCAGCGTCTCTCTGGTAACTCACTGATACTCT 57.9 0.53
mm3_intron_Arhgap15_chr2_44384698_R GTCTCGTGGGCTCGGTCCAAGACATCCCGCTTCAC 60.0 0.53
mm4_exon_Inpp5d_chr1_87667917_F TCGTCGGCAGCGTCTGTCATGTCACCACCTGAGC 59.9 0.52
mm4_exon_Inpp5d_chr1_87667917_R GTCTCGTGGGCTCGGTCTGTTGCAAGAGGAAGGTCC 59.9 0.52
mm4_intergenic_Csnka2ip|4930453N24Rik_chr16_64553642_F TCGTCGGCAGCGTCCAGCCACCAAACCTAGATCCT 59.4 0.51
mm4_intergenic_Csnka2ip|4930453N24Rik_chr16_64553642_R GTCTCGTGGGCTCGGAACCAGAGAAGTGCTGTGCT 59.5 0.51
mm2_intergenic_Gm24815|Gm9144_chr12_7828824_F Primer3: not found at this Tm N.d. 0.50
mm2_intergenic_Gm24815|Gm9144_chr12_7828824_R Primer3: not found at this Tm N.d. 0.50
mm3_intron_Greb1_chr12_16691060_F TCGTCGGCAGCGTCGGATGGCGTGTGGAACCTAA 60.0 0.49
mm3_intron_Greb1_chr12_16691060_R GTCTCGTGGGCTCGGTCTCAGTGCATGACCTTGTCC 59.9 0.49
mm4_intergenic_Gm11298|Vmn1r201_chr13_22472139_F Primer3: not found at this Tm N.d. 0.48
mm4_intergenic_Gm11298|Vmn1r201_chr13_22472139_R Primer3: not found at this Tm N.d. 0.48
mm4_intron_Myo18b_chr5_112732104_F Primer3: not found at this Tm N.d. 0.47
mm4_intron_Myo18b_chr5_112732104_R Primer3: not found at this Tm N.d. 0.47
mm4_intron_2310061I04Rik_chr17_35893792_F TCGTCGGCAGCGTCGCTTGAGCCTAATAATTTTGGGG 58.0 0.46
mm4_intron_2310061I04Rik_chr17_35893792_R GTCTCGTGGGCTCGGCCAGTCTGGCATGCATGAAC 59.5 0.46
mm4_intergenic_Nox3|Gm23186_chr17_4616050_F Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_Nox3|Gm23186_chr17_4616050_R Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_Slc24a3|Gm22390_chr2_145449713_F TCGTCGGCAGCGTCATCCTTGTTGCTGGGCTCTC 60.0 0.45
mm4_intergenic_Slc24a3|Gm22390_chr2_145449713_R GTCTCGTGGGCTCGGCCAGGTCTGAGCGTGAAGAA 59.6 0.45
mm4_intron_Msra_chr14_64268451_F TCGTCGGCAGCGTCTAACTCTGTCCGTGAGCTGC 59.7 0.44
mm4_intron_Msra_chr14_64268451_R GTCTCGTGGGCTCGGCCTGACACTTCCTGGGAGGA 60.5 0.44
mm4_intron_Myo5a_chr9_75195263_F Primer3: not found at this Tm N.d. 0.42
mm4_intron_Myo5a_chr9_75195263_R Primer3: not found at this Tm N.d. 0.42
mm4_intron_Sgms1_chr19_32231674_F Primer3: not found at this Tm N.d. 0.42
mm4_intron_Sgms1_chr19_32231674_R Primer3: not found at this Tm N.d. 0.42
mm4_intergenic_Gm10827|Nhsl1_chr10_18381369_F Primer3: not found at this Tm N.d. 0.41
mm4_intergenic_Gm10827|Nhsl1_chr10_18381369_R Primer3: not found at this Tm N.d. 0.41
mm4_intergenic_Cdh7|Cdh19_chr1_110609910_F Primer3: not found at this Tm N.d. 0.41
mm4_intergenic_Cdh7|Cdh19_chr1_110609910_R Primer3: not found at this Tm N.d. 0.41
mm4_intergenic_Zfy2|Gm22119_chrY_2368043_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Zfy2|Gm22119_chrY_2368043_R Primer3: not found at this Tm N.d. 0.40
mm4_intron_Gm15155_chrX_156219211_F Primer3: not found at this Tm N.d. 0.40
mm4_intron_Gm15155_chrX_156219211_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm25176|Smarca2_chr19_26596664_F Primer3: not found at this Tm N.d. 0.39
mm4_intergenic_Gm25176|Smarca2_chr19_26596664_R Primer3: not found at this Tm N.d. 0.39
mm4_intergenic_Gm16203|Sgcz_chr8_38485113_F TCGTCGGCAGCGTCTCTTCCTGCATGATGAAGACCA 59.4 0.37
mm4_intergenic_Gm16203|Sgcz_chr8_38485113_R GTCTCGTGGGCTCGGCCTTCTCAGTTCTGTGCCCC 60.3 0.37
mm4_intergenic_Gm5611|Chordc1_chr9_17629738_F Primer3: not found at this Tm N.d. 0.37
mm4_intergenic_Gm5611|Chordc1_chr9_17629738_R Primer3: not found at this Tm N.d. 0.37
mm4_intron_Cenpf_chr1_189669542_F Primer3: not found at this Tm N.d. 0.36
mm4_intron_Cenpf_chr1_189669542_R Primer3: not found at this Tm N.d. 0.36
mm4_intergenic_Gm22495|Dtna_chr18_23212617_F Primer3: not found at this Tm N.d. 0.36
mm4_intergenic_Gm22495|Dtna_chr18_23212617_R Primer3: not found at this Tm N.d. 0.36
mm4_intergenic_C1galt1c1|Ap3s1-ps1_chrX_38673914_F TCGTCGGCAGCGTCCTCTCCCTGGCTTTGGGTTT 59.8 0.35
mm4_intergenic_C1galt1c1|Ap3s1-ps1_chrX_38673914_R GTCTCGTGGGCTCGGACACGGGGTCATCATTCCAC 60.0 0.35
mm4_intergenic_Zfp97|Gm6712_chr17_17212802_F Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Zfp97|Gm6712_chr17_17212802_R Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Zfp97|Gm6712_chr17_17152888_F Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Zfp97|Gm6712_chr17_17152888_R Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Zfp960|Zfp97_chr17_17095677_F Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Zfp960|Zfp97_chr17_17095677_R Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_BC002059|Zfp960_chr17_17047488_F Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_BC002059|Zfp960_chr17_17047488_R Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_BC002059|Zfp960_chr17_16979657_F Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_BC002059|Zfp960_chr17_16979657_R Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Gm8993|Gm9001_chr6_75631226_F TCGTCGGCAGCGTCAGGACAGATGGCTACTGCAA 58.7 0.35
mm4_intergenic_Gm8993|Gm9001_chr6_75631226_R GTCTCGTGGGCTCGGTGCCACATTTCAGTTACCTGA 57.5 0.35
mm4_exon_Taf1d_chr9_15311856_F TCGTCGGCAGCGTCTTTTCGTTGCTTGATTTCAGAGT 57.5 0.34
mm4_exon_Taf1d_chr9_15311856_R GTCTCGTGGGCTCGGCGAGGCTGGGGTCTTGAATC 60.4 0.34
mm4_intergenic_Gm11116|Gm11115_chr5_88125342_F Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Gm11116|Gm11115_chr5_88125342_R Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Gm6317|Gm25383_chr13_79027742_F Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Gm6317|Gm25383_chr13_79027742_R Primer3: not found at this Tm N.d. 0.31
mm4_intron_Zranb3_chr1_128003766_F TCGTCGGCAGCGTCTCACTTGAATGGAAGAATGGGA 57.3 0.31
mm4_intron_Zranb3_chr1_128003766_R GTCTCGTGGGCTCGGACCTCCGTGCATCTCTGTTC 59.7 0.31
mm4_intergenic_Gm23661|Gm23067_chr5_70079707_F Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Gm23661|Gm23067_chr5_70079707_R Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Casp12|Pdgfd_chr9_5770906_F TCGTCGGCAGCGTCACCGCAGACACTTTATAGGAGA 58.6 0.30
mm4_intergenic_Casp12|Pdgfd_chr9_5770906_R GTCTCGTGGGCTCGGACAATCACCAAAGGTCTTTGCA 58.9 0.30
mm4_intergenic_Id2|Gm22527_chr12_25231788_F Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Id2|Gm22527_chr12_25231788_R Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Cat|Gm13996_chr2_103498821_F Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Cat|Gm13996_chr2_103498821_R Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Gm15577|Negr1_chr3_156977288_F TCGTCGGCAGCGTCGCATACTTCCCTGGAGCTGG 60.1 0.28
mm4_intergenic_Gm15577|Negr1_chr3_156977288_R GTCTCGTGGGCTCGGGCAGGCAGCTCAGATCTGTC 60.8 0.28
mm4_intergenic_n-R5s30|Rbfox1_chr16_5892746_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_n-R5s30|Rbfox1_chr16_5892746_R Primer3: not found at this Tm N.d. 0.28
mm3_intron_Hecw1_chr13_14270687_F Primer3: not found at this Tm N.d. 0.28
mm3_intron_Hecw1_chr13_14270687_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_D730003I15Rik|Nenf_chr1_191241425_F TCGTCGGCAGCGTCGCTCTGACTTGGTAGCTAGCT 59.2 0.28
mm4_intergenic_D730003I15Rik|Nenf_chr1_191241425_R GTCTCGTGGGCTCGGGCAGATGCTGGAGTACCTACG 60.2 0.28
mm4_intergenic_Oxnad1|Msmb_chr14_32133883_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Oxnad1|Msmb_chr14_32133883_R Primer3: not found at this Tm N.d. 0.27
mm3_intergenic_Nlgn1|Gm24250_chr3_25541165_F TCGTCGGCAGCGTCAGCTGTGTGAGTTGACATGTCA 60.1 0.27
mm3_intergenic_Nlgn1|Gm24250_chr3_25541165_R GTCTCGTGGGCTCGGTTGTCAGGGGATGGTGAAGC 59.9 0.27
mm4_intergenic_Gm6931|Ift57_chr16_49632709_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm6931|Ift57_chr16_49632709_R Primer3: not found at this Tm N.d. 0.27
mm4_intron_Arhgap29_chr3_122001887_F Primer3: not found at this Tm N.d. 0.27
mm4_intron_Arhgap29_chr3_122001887_R Primer3: not found at this Tm N.d. 0.27
mm4_intron_Ptprk_chr10_28431496_F Primer3: not found at this Tm N.d. 0.26
mm4_intron_Ptprk_chr10_28431496_R Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Gm22016|Cdc73_chr1_141952495_F TCGTCGGCAGCGTCGCACATGATTTGCAATGCACA 58.9 0.26
mm4_intergenic_Gm22016|Cdc73_chr1_141952495_R GTCTCGTGGGCTCGGACATATGTTGGAATTTGCATTGACA 58.3 0.26
mm4_intergenic_Nrcam|Stxbp6_chr12_44670725_F TCGTCGGCAGCGTCCTCATGACCTCTGCCACTCC 59.8 0.26
mm4_intergenic_Nrcam|Stxbp6_chr12_44670725_R GTCTCGTGGGCTCGGTGTCCTCAGGAACCAGAAGA 57.6 0.26
mm4_intron_Ctnna2_chr6_76989781_F TCGTCGGCAGCGTCCCCATGCATTCGACCTCCAA 60.3 0.26
mm4_intron_Ctnna2_chr6_76989781_R GTCTCGTGGGCTCGGTGGACCTCTGAGTTGCTACT 57.6 0.26
mm3_intergenic_Gm26407|Impact_chr18_12963039_F Primer3: not found at this Tm N.d. 0.24
mm3_intergenic_Gm26407|Impact_chr18_12963039_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Rab3c|Gm5455_chr13_110287964_F Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Rab3c|Gm5455_chr13_110287964_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm11146|Mir125b-2_chr16_77622019_F TCGTCGGCAGCGTCTCAGGTGAATTCTTTGTTACTTCCA 58.6 0.23
mm4_intergenic_Gm11146|Mir125b-2_chr16_77622019_R GTCTCGTGGGCTCGGCAGAGGCACAATACAGGGCA 60.0 0.23
mm4_intron_Klhl32_chr4_24623792_F TCGTCGGCAGCGTCACATATGTACTCATACACAGACACA 57.5 0.22
mm4_intron_Klhl32_chr4_24623792_R GTCTCGTGGGCTCGGATGTGGCCTGGTAGCACAAG 60.3 0.22
mm4_intergenic_Gm22939|Cntnap2_chr6_44992904_F TCGTCGGCAGCGTCAGACTGATGCAAAATTTACCCTTGA 59.2 0.22
mm4_intergenic_Gm22939|Cntnap2_chr6_44992904_R GTCTCGTGGGCTCGGTGGTCTCCATAGTAATAAGGTCCT 57.9 0.22
mm4_intergenic_Gm8577|Gm16390_chrX_154850013_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gm8577|Gm16390_chrX_154850013_R Primer3: not found at this Tm N.d. 0.22
mm3_intergenic_Mir3964|Gm23033_chr15_29727962_F Primer3: not found at this Tm N.d. 0.21
mm3_intergenic_Mir3964|Gm23033_chr15_29727962_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm21820|AC132591.1_chrY_5355572_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm21820|AC132591.1_chrY_5355572_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_AC121988.1|Gm25740_chr10_46198704_F TCGTCGGCAGCGTCGCTTGTACATTTCCCCAGTGG 59.1 0.21
mm4_intergenic_AC121988.1|Gm25740_chr10_46198704_R GTCTCGTGGGCTCGGTCAGGAAGACAACAAAAGGCA 57.9 0.21
mm4_intron_Gm14461_chr2_78261986_F TCGTCGGCAGCGTCAATGGCAACCAGGAACCCTT 59.8 0.20
mm4_intron_Gm14461_chr2_78261986_R GTCTCGTGGGCTCGGCCCGTGCAATTTAATTCAAGGC 59.0 0.20
mm3_intergenic_Plcl1|Hsfy2_chr1_56483951_F TCGTCGGCAGCGTCTGGCCTCCAACACATTGACT 59.5 0.20
mm3_intergenic_Plcl1|Hsfy2_chr1_56483951_R GTCTCGTGGGCTCGGCTTGTCTGTCTGGGTGCAGA 59.6 0.20
mm4_intron_Arl6ip5_chr6_97213121_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Arl6ip5_chr6_97213121_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm5446|Elmo1_chr13_20001161_F TCGTCGGCAGCGTCGCTTCCTGAAAATGCCCCTG 59.4 0.19
mm4_intergenic_Gm5446|Elmo1_chr13_20001161_R GTCTCGTGGGCTCGGGGATAGGCTTCTTCCTCCTTGG 59.8 0.19
mm4_intron_Runx1_chr16_92743053_F Primer3: not found at this Tm N.d. 0.18
mm4_intron_Runx1_chr16_92743053_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Ube2e2_chr14_18614306_F TCGTCGGCAGCGTCGGTGGAACCATCTGAGCTGT 59.6 0.18
mm4_intron_Ube2e2_chr14_18614306_R GTCTCGTGGGCTCGGTGCCTGCCCACTTCACTTTA 59.5 0.18
mm4_intergenic_Nid1|Lyst_chr13_13581291_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Nid1|Lyst_chr13_13581291_R Primer3: not found at this Tm N.d. 0.18
mm3_intergenic_Smr3a|Smr2_chr5_88008595_F Primer3: not found at this Tm N.d. 0.18
mm3_intergenic_Smr3a|Smr2_chr5_88008595_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm11751|Gm23738_chr4_68168070_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm11751|Gm23738_chr4_68168070_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm23425|Gm22483_chr18_40931335_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm23425|Gm22483_chr18_40931335_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Ifng|Dyrk2/4932442E05Rik_chr10_118485786_F TCGTCGGCAGCGTCTGCTGAATCACGTGTTGAGC 59.1 0.16
mm4_intergenic_Ifng|Dyrk2/4932442E05Rik_chr10_118485786_R GTCTCGTGGGCTCGGTGGTGTGGTGTCTGTAGGGA 60.1 0.16
mm4_intron_0610040F04Rik_chr6_108613255_F TCGTCGGCAGCGTCGCTGGTCAAAACATAAGTGCCA 59.7 0.16
mm4_intron_0610040F04Rik_chr6_108613255_R GTCTCGTGGGCTCGGGTGAATAGGGTGGGGTTGGG 60.0 0.16
mm4_intron_Osbpl3_chr6_50378007_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Osbpl3_chr6_50378007_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Cenpc1_chr5_86040379_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Cenpc1_chr5_86040379_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Spag17|Wdr3_chr3_100135965_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Spag17|Wdr3_chr3_100135965_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Ccdc176_chr12_84411500_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Ccdc176_chr12_84411500_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Actn1_chr12_80189395_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Actn1_chr12_80189395_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm22689|Gm9742_chr13_7311644_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm22689|Gm9742_chr13_7311644_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm24633|Gm25568_chr1_19875326_F TCGTCGGCAGCGTCACAGGTCAGGGGACATCTCA 59.8 0.14
mm4_intergenic_Gm24633|Gm25568_chr1_19875326_R GTCTCGTGGGCTCGGTGTTGGTGATCAAACACAGGA 57.7 0.14
mm4_intron_Gpc6_chr14_117384716_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Gpc6_chr14_117384716_R Primer3: not found at this Tm N.d. 0.14
mm3_intron_Spock1_chr13_57535947_F Primer3: not found at this Tm N.d. 0.14
mm3_intron_Spock1_chr13_57535947_R Primer3: not found at this Tm N.d. 0.14
mm3_intergenic_Ndfip1|Spry4_chr18_38559899_F Primer3: not found at this Tm N.d. 0.14
mm3_intergenic_Ndfip1|Spry4_chr18_38559899_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Leo1|Tmod3_chr9_75481547_F TCGTCGGCAGCGTCAGGAGGCAGAAGCGGCAG 62.0 0.14
mm4_intergenic_Leo1|Tmod3_chr9_75481547_R GTCTCGTGGGCTCGGACGGCTTTTAGTGGCAACCT 60.1 0.14
mm4_intron_Gm12436_chr4_48904256_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Gm12436_chr4_48904256_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_4930432M17Rik_chr3_121679725_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_4930432M17Rik_chr3_121679725_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm24477|Trp53rk_chr2_166773441_F TCGTCGGCAGCGTCGCTGGTCTAGGATGCGAGTC 59.9 0.13
mm4_intergenic_Gm24477|Trp53rk_chr2_166773441_R GTCTCGTGGGCTCGGAGGAGACTGCAGGCTATCCC 60.7 0.13
mm4_intron_Fbrsl1_chr5_110428136_F TCGTCGGCAGCGTCGCAGACACCTCCTTTCACCA 59.8 0.13
mm4_intron_Fbrsl1_chr5_110428136_R GTCTCGTGGGCTCGGGGGCATATGACAGAGGGAGC 59.9 0.13
mm4_intron_Depdc5_chr5_32952103_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Depdc5_chr5_32952103_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm25976|Acot10_chr15_20538753_F TCGTCGGCAGCGTCACATCTCCTAAGTGTCACCTACC 58.9 0.13
mm4_intergenic_Gm25976|Acot10_chr15_20538753_R GTCTCGTGGGCTCGGTGTGTACTTTCCTAATGTGACTGA 57.5 0.13
mm4_intergenic_Smr3a|Smr2_chr5_88026693_F TCGTCGGCAGCGTCAAATGAAGAGCTGAACGGCA 58.1 0.12
mm4_intergenic_Smr3a|Smr2_chr5_88026693_R GTCTCGTGGGCTCGGAATGACCACTTAGCCTTCTGG 57.6 0.12
mm4_intergenic_Usp7|1810013L24Rik_chr16_8800410_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Usp7|1810013L24Rik_chr16_8800410_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_E130309F12Rik_chr4_49078477_F TCGTCGGCAGCGTCTCACCTTCAGAAAGCCTTCCA 59.2 0.12
mm4_intron_E130309F12Rik_chr4_49078477_R GTCTCGTGGGCTCGGGGTGTGGTAGCGGAATGACA 60.0 0.12
mm4_intergenic_Apoo-ps|Zswim6_chr13_107503189_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Apoo-ps|Zswim6_chr13_107503189_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm11499|Gm11500_chr11_92491424_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm11499|Gm11500_chr11_92491424_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm11115|Gm7714_chr5_88185882_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm11115|Gm7714_chr5_88185882_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm26913|Gm4076_chr13_84861963_F TCGTCGGCAGCGTCCCTCCTTTAGTTTGCGCCTC 58.9 0.12
mm4_intergenic_Gm26913|Gm4076_chr13_84861963_R GTCTCGTGGGCTCGGAAAGGAGCAGTTCAGGGCAA 59.8 0.12
mm4_intergenic_Gm11115|Gm7714_chr5_88254667_F TCGTCGGCAGCGTCGCACGCACTTGCAGTCATTA 59.4 0.12
mm4_intergenic_Gm11115|Gm7714_chr5_88254667_R GTCTCGTGGGCTCGGCCTGATAACCAGTTGGCTTCCT 60.0 0.12
mm4_intron_Sulf1_chr1_12818798_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Sulf1_chr1_12818798_R Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_Gm23558|Galc_chr12_97974205_F TCGTCGGCAGCGTCACGAATGCAACGGTAGTTTGC 60.0 0.12
mm3_intergenic_Gm23558|Galc_chr12_97974205_R GTCTCGTGGGCTCGGAGGACACAAATGCTCCCCAG 59.9 0.12
mm4_intergenic_Gm8261|Gm15075_chrX_146639839_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm8261|Gm15075_chrX_146639839_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm24298|4921513I03Rik_chr10_120657598_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm24298|4921513I03Rik_chr10_120657598_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm23526|Grik4_chr9_42933361_F TCGTCGGCAGCGTCTATGGCAAGCACTCCAAGGG 60.0 0.12
mm4_intergenic_Gm23526|Grik4_chr9_42933361_R GTCTCGTGGGCTCGGCCTTTCCTGATGCTCTTCCCC 60.4 0.12
mm4_intergenic_Gm7729|Gm24246_chr18_28156554_F TCGTCGGCAGCGTCCCAGTTCCCTTGTCTCTGCC 60.3 0.11
mm4_intergenic_Gm7729|Gm24246_chr18_28156554_R GTCTCGTGGGCTCGGAAGGTGCTCGGCTGAAGAAA 59.8 0.11
mm4_intron_Arhgef3_chr14_27286010_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Arhgef3_chr14_27286010_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Whrn|Atp6v1g1_chr4_63533243_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Whrn|Atp6v1g1_chr4_63533243_R Primer3: not found at this Tm N.d. 0.11
mm3_exon_Kcnq5_chr1_21398608_F Primer3: not found at this Tm N.d. 0.11
mm3_exon_Kcnq5_chr1_21398608_R Primer3: not found at this Tm N.d. 0.11
mm3_intergenic_Ssx9|Ssxb5_chrX_8800335_F Primer3: not found at this Tm N.d. 0.11
mm3_intergenic_Ssx9|Ssxb5_chrX_8800335_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Vwa8_chr14_78964086_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Vwa8_chr14_78964086_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Abhd12b_chr12_70163687_F TCGTCGGCAGCGTCGTGAAGGTGTTCTGGCAAACA 59.2 0.11
mm4_intron_Abhd12b_chr12_70163687_R GTCTCGTGGGCTCGGAGAAACCAGTGGCTTCATTGA 57.7 0.11
mm3_intergenic_mmu-mir-6393|Zdhhc25_chr15_88128812_F TCGTCGGCAGCGTCGGGTTCACCTCCCTGTGTTC 60.2 0.11
mm3_intergenic_mmu-mir-6393|Zdhhc25_chr15_88128812_R GTCTCGTGGGCTCGGAGAGCTCTGACCTGACCTGT 59.8 0.11
mm4_intergenic_Gm22483|Prelid2_chr18_41099430_F TCGTCGGCAGCGTCTCCCATAGGAATTCAAGACAAGCT 59.7 0.10
mm4_intergenic_Gm22483|Prelid2_chr18_41099430_R GTCTCGTGGGCTCGGTGCTGAGTATCTACTGCCTCTCT 60.1 0.10
mm3_intergenic_Cryge|Crygd_chr1_65053554_F Primer3: not found at this Tm N.d. 0.10
mm3_intergenic_Cryge|Crygd_chr1_65053554_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Runx1_chr16_92818221_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_Runx1_chr16_92818221_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm22254|Gm8993_chr6_74532236_F TCGTCGGCAGCGTCTCCACATTCAAGGAACTCTCTCC 59.7 0.10
mm4_intergenic_Gm22254|Gm8993_chr6_74532236_R GTCTCGTGGGCTCGGACAGAACAAGAAGCAGAACTGT 58.1 0.10
mm4_intergenic_Il1rapl2|Gm25393_chrX_137767947_F TCGTCGGCAGCGTCCCTAGTGCCAGAGAGCCCTA 60.1 0.09
mm4_intergenic_Il1rapl2|Gm25393_chrX_137767947_R GTCTCGTGGGCTCGGGGGGCTAAAGGTAGAGTCATGA 58.9 0.09
mm4_intron_Tmem27_chrX_164104253_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Tmem27_chrX_164104253_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm7847|Gm23674_chr18_26343334_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm7847|Gm23674_chr18_26343334_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_4930435H24Rik|Qdpr_chr5_45352631_F TCGTCGGCAGCGTCATCTTATGACCCTACTATGTGCAT 57.0 0.09
mm4_intergenic_4930435H24Rik|Qdpr_chr5_45352631_R GTCTCGTGGGCTCGGTGCCCTTTCAGCTGAAGAGT 59.2 0.09
mm4_intergenic_Gm5432|Lpin1_chr12_16277297_F TCGTCGGCAGCGTCCTGACACCCTGCACAGCATT 60.8 0.08
mm4_intergenic_Gm5432|Lpin1_chr12_16277297_R GTCTCGTGGGCTCGGAAGTTCACCAGGCCTGACAC 60.1 0.08
mm4_intergenic_Gm13018|Prdm2_chr4_142902302_F TCGTCGGCAGCGTCTCTCCAGTGTGAGAACGGAA 58.3 0.08
mm4_intergenic_Gm13018|Prdm2_chr4_142902302_R GTCTCGTGGGCTCGGATGTGAACGCGTCTCCAGAC 60.3 0.08
mm4_intergenic_Smr3a|Smr2_chr5_88075525_F TCGTCGGCAGCGTCAGCTGGGCATCACTATATTGCA 59.8 0.08
mm4_intergenic_Smr3a|Smr2_chr5_88075525_R GTCTCGTGGGCTCGGCCCTAATAACCAATTTGTCTCCTGG 59.4 0.08
mm4_intergenic_Irx1|Rpl9-ps4_chr13_72217015_F TCGTCGGCAGCGTCCTCTACAGGATGCTGGCCAG 59.8 0.08
mm4_intergenic_Irx1|Rpl9-ps4_chr13_72217015_R GTCTCGTGGGCTCGGAGGGAATTATGAAGGCAACCCT 59.4 0.08
mm4_intergenic_Rdh14|Pgk1-rs7_chr12_10542962_F TCGTCGGCAGCGTCCTCGTACAGGGGCCAAAGAT 59.4 0.08
mm4_intergenic_Rdh14|Pgk1-rs7_chr12_10542962_R GTCTCGTGGGCTCGGTCAGGACCAGGTTTCCCTCT 59.8 0.08
mm4_intron_Pax7_chr4_139827795_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Pax7_chr4_139827795_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Agbl1_chr7_76581678_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Agbl1_chr7_76581678_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Ssxb9|Ssxb1_chrX_8411791_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Ssxb9|Ssxb1_chrX_8411791_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Ssxb8|Ssx9_chrX_8701370_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Ssxb8|Ssx9_chrX_8701370_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Ssxb6|Ssxb3_chrX_8558129_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Ssxb6|Ssxb3_chrX_8558129_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Ssxb10|Ssxb9_chrX_8366170_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Ssxb10|Ssxb9_chrX_8366170_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Slc38a5|Ssxb10_chrX_8326618_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Slc38a5|Ssxb10_chrX_8326618_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm6592|Gm5751_chrX_8873217_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm6592|Gm5751_chrX_8873217_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Inppl1_chr7_101828916_F TCGTCGGCAGCGTCCGTGAAGTCCACAGCTCCAA 60.2 0.07
mm4_intron_Inppl1_chr7_101828916_R GTCTCGTGGGCTCGGGGAGCGGGAGAAGCATAAGG 60.2 0.07
mm4_intron_Sorbs1_chr19_40393336_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Sorbs1_chr19_40393336_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm24319|Psat1_chr19_15697949_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm24319|Psat1_chr19_15697949_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm23067|Gm26072_chr5_70240084_F TCGTCGGCAGCGTCAGTTTGGTTGGTCCCTGTGC 60.7 0.07
mm4_intergenic_Gm23067|Gm26072_chr5_70240084_R GTCTCGTGGGCTCGGAGGTAGAAACAAATTCCTCAGGACA 59.9 0.07
mm4_intergenic_Gm13579|Gm13581_chr2_61109683_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm13579|Gm13581_chr2_61109683_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Egfr_chr11_16870898_F TCGTCGGCAGCGTCTAGCAGCCCATACACAGCAG 59.8 0.07
mm4_intron_Egfr_chr11_16870898_R GTCTCGTGGGCTCGGAGCCATGATCTGTCACCACG 60.1 0.07
mm4_intergenic_Gm22813|Mpped2_chr2_106826913_F TCGTCGGCAGCGTCCCAAATCCCAGCTCCTCTGT 59.3 0.06
mm4_intergenic_Gm22813|Mpped2_chr2_106826913_R GTCTCGTGGGCTCGGTGTTACAAGAGCTATCAGGGCA 59.1 0.06
mm4_intergenic_Gm25169|Efnb2_chr8_8031146_F TCGTCGGCAGCGTCTGGTTGCACAGAGTTACGTCA 59.8 0.06
mm4_intergenic_Gm25169|Efnb2_chr8_8031146_R GTCTCGTGGGCTCGGAGGCTGGAGGCATAAACTGA 58.7 0.06
mm4_intergenic_Slc22a23|Pxdc1_chr13_34499968_F TCGTCGGCAGCGTCTGAGCTTGGTCCTCAGATAGC 59.2 0.06
mm4_intergenic_Slc22a23|Pxdc1_chr13_34499968_R GTCTCGTGGGCTCGGACTTCTTGTGAGACCAAAGCTG 58.7 0.06
mm4_intergenic_Slc1a1/AC163993.1|AC163993.1/Slc1a1_chr19_28896779_F TCGTCGGCAGCGTCTCACATTGCTCTCCACAGCC 60.3 0.06
mm4_intergenic_Slc1a1/AC163993.1|AC163993.1/Slc1a1_chr19_28896779_R GTCTCGTGGGCTCGGAGCTCCAGAGGCACCTCATA 60.0 0.06
mm4_intergenic_Scn9a|Scn7a_chr2_66670498_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Scn9a|Scn7a_chr2_66670498_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Mms22l_chr4_24588800_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Mms22l_chr4_24588800_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm4795|Prep_chr10_45049888_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm4795|Prep_chr10_45049888_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Rcsd1|Creg1_chr1_165725577_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Rcsd1|Creg1_chr1_165725577_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Vrk2|Gm12071_chr11_26666224_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Vrk2|Gm12071_chr11_26666224_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Pcdh9_chr14_93432672_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Pcdh9_chr14_93432672_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Nbas_chr12_13543321_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Nbas_chr12_13543321_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm15496|Kcnmb2_chr3_31599367_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm15496|Kcnmb2_chr3_31599367_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Actrt2|Gm13113_chr4_154818892_F TCGTCGGCAGCGTCTCAGACATACGGCCTGATGC 59.8 0.05
mm4_intergenic_Actrt2|Gm13113_chr4_154818892_R GTCTCGTGGGCTCGGTCCTTAGGGTCCCCATCAGG 60.0 0.05
mm4_intron_Stox1_chr10_62713802_F TCGTCGGCAGCGTCATTACACCAAAACACGGCGG 59.4 0.05
mm4_intron_Stox1_chr10_62713802_R GTCTCGTGGGCTCGGACTGTTATGGTGGTCGAGGC 59.7 0.05
mm4_intergenic_Nrg3|Gm10713_chr14_39901279_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Nrg3|Gm10713_chr14_39901279_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Macf1_chr4_123350937_F TCGTCGGCAGCGTCGAGGCAGTGGTGGTCTTCTT 59.6 0.04
mm4_exon_Macf1_chr4_123350937_R GTCTCGTGGGCTCGGTCGGCTTGCTCAGACACTTC 60.3 0.04
mm4_intron_Zdhhc24_chr19_4880962_F TCGTCGGCAGCGTCTCTGATTTGATGGGGCGAGG 59.8 0.04
mm4_intron_Zdhhc24_chr19_4880962_R GTCTCGTGGGCTCGGACGGAGGTGTCAGAGGAAAG 59.0 0.04
mm4_intergenic_Sh3rf1|Cbr4_chr8_61471945_F TCGTCGGCAGCGTCAGGGGCAGAGAGATACGTAGA 59.2 0.04
mm4_intergenic_Sh3rf1|Cbr4_chr8_61471945_R GTCTCGTGGGCTCGGAAGTAAGTGGCACCCCTGTG 59.8 0.04
mm3_intergenic_Zfyve20|Trh_chr6_92220552_F TCGTCGGCAGCGTCGTCTTGGTCATGCTCACCCT 59.6 0.04
mm3_intergenic_Zfyve20|Trh_chr6_92220552_R GTCTCGTGGGCTCGGGCACATGAAGACCTTGGGGA 59.9 0.04
mm4_intergenic_Sprr1b|Sprr3_chr3_92439617_F TCGTCGGCAGCGTCGAGACTGGCAAGGTGAGCAT 60.0 0.04
mm4_intergenic_Sprr1b|Sprr3_chr3_92439617_R GTCTCGTGGGCTCGGGGTTAGTGCTGTCCTCGCTT 60.0 0.04
mm3_intron_Gm15155_chrX_156186535_F Primer3: not found at this Tm N.d. 0.04
mm3_intron_Gm15155_chrX_156186535_R Primer3: not found at this Tm N.d. 0.04
mm3_exon_Vta1_chr10_14684124_F TCGTCGGCAGCGTCAAAGGCTGGCAGTGTAGAAG 57.8 0.04
mm3_exon_Vta1_chr10_14684124_R GTCTCGTGGGCTCGGACGGGTACATGACTACATTTCAGT 59.7 0.04
mm4_intron_Dnhd1_chr7_105697872_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Dnhd1_chr7_105697872_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Bicc1_chr10_71077810_F TCGTCGGCAGCGTCCAGCTCCTCCATGCCTCATT 59.8 0.04
mm4_intron_Bicc1_chr10_71077810_R GTCTCGTGGGCTCGGCCTTTGAAGGCTCATGTTGGA 58.4 0.04
mm3_intron_Nos1_chr5_117787931_F TCGTCGGCAGCGTCTGGAGAAAGAGAAGCTCAGCC 59.7 0.03
mm3_intron_Nos1_chr5_117787931_R GTCTCGTGGGCTCGGGGGGCAGGAATCAGTGTTGA 59.9 0.03
mm4_intergenic_Gm22936|Gm11450_chr2_162775433_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm22936|Gm11450_chr2_162775433_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Smim14_chr5_65515784_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Smim14_chr5_65515784_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm24221|Sstr4_chr2_148329945_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm24221|Sstr4_chr2_148329945_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Jak1_chr4_101171424_F TCGTCGGCAGCGTCCTGCTTCTGGCCACCCAATA 60.0 0.03
mm4_intron_Jak1_chr4_101171424_R GTCTCGTGGGCTCGGCCTTCTTGCTCAGTGACCCC 60.3 0.03
mm4_intergenic_Chd9|Gm6658_chr8_90894917_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Chd9|Gm6658_chr8_90894917_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Phlda1|4933440J02Rik_chr10_111569682_F TCGTCGGCAGCGTCTGGTACCCATCCCTTCCTGT 59.8 0.02
mm4_intergenic_Phlda1|4933440J02Rik_chr10_111569682_R GTCTCGTGGGCTCGGGCACAGGTCAATGTTCCTCC 58.8 0.02
mm4_intergenic_Lrrtm3|Ctnna3_chr10_64133056_F TCGTCGGCAGCGTCACAAGCTCTGCCTCATACCT 58.4 0.02
mm4_intergenic_Lrrtm3|Ctnna3_chr10_64133056_R GTCTCGTGGGCTCGGACTCAAACACAGCCTAATCTCT 57.1 0.02
mm4_intergenic_Gm13211|Gm13254_chr2_7889249_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm13211|Gm13254_chr2_7889249_R Primer3: not found at this Tm N.d. 0.02
mm3_intron_Esrrg_chr1_187760595_F Primer3: not found at this Tm N.d. 0.02
mm3_intron_Esrrg_chr1_187760595_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_D19Bwg1357e|Gm24782_chr19_27598261_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_D19Bwg1357e|Gm24782_chr19_27598261_R Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Lmo1|Stk33_chr7_109211231_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Lmo1|Stk33_chr7_109211231_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm7665|Cdh2_chr18_16538650_F TCGTCGGCAGCGTCACAGTCACCACAATATGATGGA 57.1 0.02
mm4_intergenic_Gm7665|Cdh2_chr18_16538650_R GTCTCGTGGGCTCGGGCAAGCAGCAAACAATGAATGA 58.9 0.02
mm4_intergenic_Gm5544|Notch2_chr3_97989087_F TCGTCGGCAGCGTCGAGGGGAGGGGATGGAGG 60.1 0.01
mm4_intergenic_Gm5544|Notch2_chr3_97989087_R GTCTCGTGGGCTCGGATCAAGAGGGAGGGAGGGAG 59.7 0.01
mm3_intergenic_Gm25729|Gm21741_chrY_83680283_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm25729|Gm21741_chrY_83680283_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm25359|Gm21869_chrY_74915799_F TCGTCGGCAGCGTCTTGTCAGGGGATGGTGAAGC 59.9 0.01
mm3_intergenic_Gm25359|Gm21869_chrY_74915799_R GTCTCGTGGGCTCGGAGCTGTGTGAGTTGACATGTCA 60.1 0.01
mm3_intergenic_Gm24828|Gm21785_chrY_85418963_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm24828|Gm21785_chrY_85418963_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm23863|Gm20861_chrY_86330958_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm23863|Gm20861_chrY_86330958_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm23770|Gm21881_chrY_85779385_F TCGTCGGCAGCGTCTTGTCAGGGGATGGTGAAGC 59.9 0.01
mm3_intergenic_Gm23770|Gm21881_chrY_85779385_R GTCTCGTGGGCTCGGAGCTGTGTGAGTTGACATGTCA 60.1 0.01
mm3_intergenic_Gm23052|Gm20927_chrY_54933939_F TCGTCGGCAGCGTCTTGTCAGGGGATGGTGAAGC 59.9 0.01
mm3_intergenic_Gm23052|Gm20927_chrY_54933939_R GTCTCGTGGGCTCGGAGCTGTGTGAGTTGACATGTCA 60.1 0.01
mm3_intergenic_Gm22832|Gm21076_chrY_74754083_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm22832|Gm21076_chrY_74754083_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm22624|Gm20863_chrY_83817534_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm22624|Gm20863_chrY_83817534_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Msx1|Stx18_chr5_38003948_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Msx1|Stx18_chr5_38003948_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_RP23-448C3.1/Trpm3|Trpm3_chr19_22484909_F TCGTCGGCAGCGTCACACAGACAGAAATGCACCT 57.3 0.01
mm4_intergenic_RP23-448C3.1/Trpm3|Trpm3_chr19_22484909_R GTCTCGTGGGCTCGGGCCACATCTGCCTACACACT 60.0 0.01
mm3_intron_Ulk2_chr11_61814530_F Primer3: not found at this Tm N.d. 0.01
mm3_intron_Ulk2_chr11_61814530_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Smr2|Gm11116_chr5_88109115_F TCGTCGGCAGCGTCAGCATCCACTGTTGTCCTCA 58.9 0.01
mm3_intergenic_Smr2|Gm11116_chr5_88109115_R GTCTCGTGGGCTCGGTCCTAATGACCATTTGTCTTCTGG 58.5 0.01
mm4_intergenic_Gm12162|Gm22284_chr11_45532797_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm12162|Gm22284_chr11_45532797_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fam198b|Gm6706_chr3_79952242_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fam198b|Gm6706_chr3_79952242_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm25684|Gm9966_chr7_95480949_F TCGTCGGCAGCGTCTGAGCAAGCAGTGAGATGAG 57.6 0.01
mm4_intergenic_Gm25684|Gm9966_chr7_95480949_R GTCTCGTGGGCTCGGAGACATGGCTGCTCCTACAG 59.1 0.01
mm3_exon_Cdr1_chrX_61183680_F TCGTCGGCAGCGTCTCAACGTCTTCCACAAAATTCAGG 59.9 0.00
mm3_exon_Cdr1_chrX_61183680_R GTCTCGTGGGCTCGGGATGAGCTGGAAGACCTGGA 58.8 0.00
mm4_intergenic_Ndst4|Ugt8a_chr3_125827361_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ndst4|Ugt8a_chr3_125827361_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Dnah17_chr11_118033339_F TCGTCGGCAGCGTCGCCTGATCACATGACTGCCT 60.1 0.00
mm4_intron_Dnah17_chr11_118033339_R GTCTCGTGGGCTCGGTGACAGACAGGTGGTGATGC 59.9 0.00
mm4_intron_Tacc2_chr7_130725742_F TCGTCGGCAGCGTCGCTTGCATAGTGATTGCAGGG 59.9 0.00
mm4_intron_Tacc2_chr7_130725742_R GTCTCGTGGGCTCGGAGGCAAGGAAAGATGAGGCC 60.0 0.00
mm4_intron_Ptprn2_chr12_117165057_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ptprn2_chr12_117165057_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nid2_chr14_19800230_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nid2_chr14_19800230_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_9830107B12Rik_chr17_48130329_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_9830107B12Rik_chr17_48130329_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Grxcr1|Gm23605_chr5_68405776_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Grxcr1|Gm23605_chr5_68405776_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm8177|Tm4sf1_chr3_57271525_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm8177|Tm4sf1_chr3_57271525_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm4922|Perp_chr10_18842006_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm4922|Perp_chr10_18842006_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22527|Rnf144a_chr12_25934938_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22527|Rnf144a_chr12_25934938_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22254|Gm8993_chr6_74803399_F TCGTCGGCAGCGTCGCCCTACAGGAAACATTGCC 59.1 0.00
mm4_intergenic_Gm22254|Gm8993_chr6_74803399_R GTCTCGTGGGCTCGGTGCTGGCACTTGTATTGATATACA 58.3 0.00
mm4_intergenic_Diras2|Syk_chr13_52536574_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Diras2|Syk_chr13_52536574_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Timp2_chr11_118303519_F TCGTCGGCAGCGTCGAAGGGGTATGGCAGGGAAC 60.1 0.00
mm4_exon_Timp2_chr11_118303519_R GTCTCGTGGGCTCGGACGCTAGTGTGATTCTGCCC 60.1 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm3_intergenic_Cd274|Pdcd1lg2_chr19_29400401 ACCCTTGTAAGTGGATCGAGTCCTTTCTGAATTCTAGATCAATAATGTTTAATTTGTTCCAAAAGTCGTCATCATTTCAT
GATATAGCCTGGAAAGACACGGTCATGTTTCTGGGTGGCCTGATCCAAAGCTTTCAGC
mm4_intron_Rnf152_chr1_105304855 ACCACTTCTGCAATCCCAGTTGATCTCTGACATCATCTCCCCCTTCCATAAACATAACAGTTTCTCACTGGCCCCCACTT
CATTGAAAAAAAATAAATACTCCCCTTCCCCCTTCCTTGGACCATTAGT
mm4_intergenic_Sphkap|Gm25754_chr1_83586993 CAGTGGTTCCAGGAAGTCCATGAGAAAATCAGAGGGATTCATAAGGAAAGCCAGGTTTCCCCCCAGAAATATATCCATGT
A
TCTATTTACAGTCCATTGAGACTCCTTGTCTTTGTGGAGCCT
mm4_intron_Bcor_chrX_12096525 TTTCTTGGTCAGAGGAGCCGAGTGGCTCACTTGCATGGGCATGTTTCTGGAAGGCTCTAGGTGTCCTAGCCTTCCCGAGG
GCAGCAGAAAATCATCCACAGGAGGTGACAGCAGGAGCCCTTAG
mm3_intron_Arhgap15_chr2_44384698 TCTCTGGTAACTCACTGATACTCTAAAAATATGAGACTCCCTTCCAGAAACGTAGCCATGCCAACAATACTCACAGGTAC
AAATGTTCTCCATTTAAAGCAGGAGGAAAAGAAAAATGTGAAGCGGGATGTCTTGGA
mm4_exon_Inpp5d_chr1_87667917 TGTCATGTCACCACCTGAGCTGCCTCCCAGAAACATTCCTATGTCTGCCGGGCCCAGCGAGGCCAAGGACCTTCCTCTTG
CAACAGA
mm4_intergenic_Csnka2ip|4930453N24Rik_chr16_64553642 CAGCCACCAAACCTAGATCCTTGTGTGTACTAGACAAGCCCTCTAGAAACTTAGCTATGTCCACATTTGGTGATTTTTCT
AACGAGAAAAGAAATATAGAAGCAAGCTGTCTATTCAGATAGCACAGCACTTCTCTGGTT
mm3_intron_Greb1_chr12_16691060 GGATGGCGTGTGGAACCTAATCACTGTCAATCTACAGCTTTTGGGGGACCTCTACTATATCTAAAAAGCTTCTGACATGG
CTATATTTCAGGATGG
CTCTAATAGGACAAGGTCATGCACTGAGA
mm4_intron_2310061I04Rik_chr17_35893792 GCTTGAGCCTAATAATTTTGGGGGATGTACCATCCAGAAACTTAATCACGTACTCTACCTAGAGTTAAATTCAGGACACA
GTGTTTTTGCTCACTGGTTTCTGAAGTGAGTTCATGCATGCCAGACTGG
mm4_intergenic_Slc24a3|Gm22390_chr2_145449713 ATCCTTGTTGCTGGGCTCTCCATGTCAACCATTCAGAAAGAGCATGGACTGCTTTTTCATGTGGCTGGCACAAGTTCACT
TGGGTGTCTTGGGCTACATGGTAATGTTTCCAGAGGGCTTCTTCACGCTCAGACCTGG
mm4_intron_Msra_chr14_64268451 TAACTCTGTCCGTGAGCTGCTGGCAGGGTGTGCTTCCCATCGTTTAGGACAGCCATCCAGAAACATAATTACGTGATTAA
GTGCTGTGTCCTCCCAGGAAGTGTCAGG
mm4_intergenic_Gm16203|Sgcz_chr8_38485113 TCTTCCTGCATGATGAAGACCATCCCCCTCACAGAAACATGTCCATGTCCACCTTGTGCTGTACACCCATGCCAGGGGCA
CAGAACTGAGAAGG
mm4_intergenic_C1galt1c1|Ap3s1-ps1_chrX_38673914 CTCTCCCTGGCTTTGGGTTTGCTTGAAGATGCTCAGAGCCTCCCCAGAGTTATGTTTCTGGAAGGAGGCTCTCAGGATCT
GACTGAATAGTGGAATGATGACCCCGTGT
mm4_intergenic_Gm8993|Gm9001_chr6_75631226 AGGACAGATGGCTACTGCAAAGACATAGCTATGTTTCATGAGGGAGAACATGTTCAGGTAACTGAAATGTGGCA
mm4_exon_Taf1d_chr9_15311856 TTTTCGTTGCTTGATTTCAGAGTAAGAAATACTATAAGGATTATTTTACTACTATAGTAAACATGATTGTGATTCTGGAA
GG
AAAATGCTAACAATCCTATACATGTCTAGATTCAAGACCCCAGCCTCG
mm4_intron_Zranb3_chr1_128003766 TCACTTGAATGGAAGAATGGGAATGAGACAGTTATGTATCTGGAGGGGAACAGAGATGCACGGAGGT
mm4_intergenic_Casp12|Pdgfd_chr9_5770906 ACCGCAGACACTTTATAGGAGAAAGCCATGATTATGTTACTGTAAGGATAATGTTCTTGTAGGATTTCCTTTTTCATAAA
TCTTGAATGTATTTTGAATCATAAAATATTTCTATGCAAAGACCTTTGGTGATTGT
mm4_intergenic_Gm15577|Negr1_chr3_156977288 GCATACTTCCCTGGAGCTGGGATTCTTGTCAGTGTCATGTTTCTGGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNCTTTCTGAAAGGTTTTAAGACAGATCTGAGCTGCCTGC
mm4_intergenic_D730003I15Rik|Nenf_chr1_191241425 GCTCTGACTTGGTAGCTAGCTCTTCTAAGTTCTCTACATTGCTTTGGAAGTTTTAGCCTATAAACTCAGGAGGAAGATCT
AAGAAAAAGCCCTCCATCCAGAAACAAAGCCAACTCAGTCGTAGGTACTCCAGCATCTGC
mm3_intergenic_Nlgn1|Gm24250_chr3_25541165 AGCTGTGTGAGTTGACATGTCATTGAACCTTCCAGAAAAAAAACCATGCCAGTATGATTTTCCCCATATCATCTAATGAA
AAGTTCGAGAGCACATTTTAACTATGTATTGTTCACAAATGCTTCACCATCCCCTGACAA
mm4_intergenic_Gm22016|Cdc73_chr1_141952495 GCACATGATTTGCAATGCACATACACCAATGTATTCAACTCACCTCCCATAAAAATAACCATGTTTTAAATAAGGAAATT
TTGTCAATGCAAATTCCAACATATGT
mm4_intergenic_Nrcam|Stxbp6_chr12_44670725 CTCATGACCTCTGCCACTCCACCTGTTCTACAAACTGCACAGGACATACTGATGTATCTGGAAGGATGTCCAAAGGTTAA
AATTAATTATCTTCTGGTTCCTGAGGACA
mm4_intron_Ctnna2_chr6_76989781 CCCATGCATTCGACCTCCAAATGCTTTCCAATTTTTCATTAATTTTTATACCTTCAGGAATCATATCCATGTCTTAAGAA
GAACTTACATTCAAAATGAAGCAAAGTAGCAACTCAGAGGTCCA
mm4_intergenic_Gm11146|Mir125b-2_chr16_77622019 TCAGGTGAATTCTTTGTTACTTCCAGTTTAGTATACTCAATCAAAAGCTAGAAACCATATTTCTAAAATGGTTATGTTTC
TTCAAGG
CATTTTGCCCTGTATTGTGCCTCTG
mm4_intron_Klhl32_chr4_24623792 ACATATGTACTCATACACAGACACACCACACAGATACATAACCATGTGGAAAATGAAACATGTCCAGAGAGATGGTTTAG
TGGTACAAGTGCTTGTGCTACCAGGCCACAT
mm4_intergenic_Gm22939|Cntnap2_chr6_44992904 AGACTGATGCAAAATTTACCCTTGAATTAAAAAATTGCTTCCTCTTTTACATTTTTTTTTGCTGTATTGTGAAATATACC
TTGAAGAAATATATCCATGTC
AGGACCTTATTACTATGGAGACCA
mm4_intergenic_AC121988.1|Gm25740_chr10_46198704 GCTTGTACATTTCCCCAGTGGATGATTTTTATTAGTGTCAAAAGATAAAGCGTATATGCATGCACACATGCTGATGTTTC
TGAAGGG
CATTGTGATGAAAGATTGGAATGCCTTTTGTTGTCTTCCTGA
mm4_intron_Gm14461_chr2_78261986 AATGGCAACCAGGAACCCTTATTCTGGAACTGGCTATGTTTCTTGATGGACATCTCAAACAAGAACATCAGATTCAACAC
GTGGACTATGTTTATTTAGGGACACAATCCTGAAAAGCCTTGAATTAAATTGCACGGG
mm3_intergenic_Plcl1|Hsfy2_chr1_56483951 TGGCCTCCAACACATTGACTCACAATGCCACAGACTGTGAATAGCTTTCCATGGCTATGTTTCTGGATAGAACCATAAAA
TATTTTCATAGATGTCATAAGGGGAAGATTTTCTATCTGCACCCAGACAGACAAG
mm4_intergenic_Gm5446|Elmo1_chr13_20001161 GCTTCCTGAAAATGCCCCTGTTTGTCTCATAAATAAGAACCATTCAGAAACAAAACCAGTTCTGTATTTTCTACAGGACT
TTCTGATAGGTTTCTCCAAGGAGGAAGAAGCCTATCC
mm4_intron_Ube2e2_chr14_18614306 GGTGGAACCATCTGAGCTGTGAGATTTTCTTCATCTTTGCCATCCCAAAGGTTCAGATATTACAGCTGGACTGGGCTTCT
GCCCTCCAGAAACCTTACCCTGGCAATTAAAGTGAAGTGGGCAGGCA
mm4_intergenic_Ifng|Dyrk2/4932442E05Rik_chr10_118485786 TGCTGAATCACGTGTTGAGCTGTATATACAAAGGTAAGAAAATGTCTTTGGTCTGACATGGCTTTGTATTTGGATGGCTC
ACAAATACAGACACATAGAGTCCCTACAGACACCACACCA
mm4_intron_0610040F04Rik_chr6_108613255 GCTGGTCAAAACATAAGTGCCAGCCTTCAAGAAACAGTTCCATGTCCTTTGTTAACCTCCATGAGAGTTCTCTGCAATGC
CCCCAACCCCACCCTATTCAC
mm4_intergenic_Gm24633|Gm25568_chr1_19875326 ACAGGTCAGGGGACATCTCAGTGCACATGGTTCTGTATATGGAAGGTCCTGTGTTTGATCACCAACA
mm4_intergenic_Leo1|Tmod3_chr9_75481547 AGGAGGCAGAAGCGGCAGCAGCGTCCAGGTCGGAGTTCCACCTTCCAGAAGCCTAACCTTGTTGTTNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNGCAAGGCCTCCATCGAGGTTGCCACTAAAAGCCGT
mm4_intergenic_Gm24477|Trp53rk_chr2_166773441 GCTGGTCTAGGATGCGAGTCTTGTTATTGTCCTACGATCAGTAAAGGACATGGGGCCGGCCATTTTTTCTGTATCTTCTG
GATGTATTTCAGAGATGGGAAGGTTTCTGGAAGGTGGGATAGCCTGCAGTCTCCT
mm4_intron_Fbrsl1_chr5_110428136 GCAGACACCTCCTTTCACCAAGACATGCTGCTCCAAGGCCCTGAGACATTATTCTGTTTCTGGTCGGACCTCACTTCCTT
TGCCAAGGTTTGGTTGCTCCCTCTGTCATATGCCC
mm4_intergenic_Gm25976|Acot10_chr15_20538753 ACATCTCCTAAGTGTCACCTACCCCCTCCTAAAACAAAACCATGTTTTATTTATTCCTTGCATGTATTATTTATTCCAAT
GGTTTTATAACAATTACATCATCAGTCACATTAGGAAAGTACACA
mm4_intergenic_Smr3a|Smr2_chr5_88026693 AAATGAAGAGCTGAACGGCATTATAAAGCATCTACTGTTGTCATCATTGTGATTACCATACAACCTACTATGTCATTTGT
ATAAAAGTAATCCTTTCAGAAACCTAACCTTGTTCCAGAAGGCTAAGTGGTCATT
mm4_intron_E130309F12Rik_chr4_49078477 TCACCTTCAGAAAGCCTTCCAGATCGATTTCATAGCCCCCAGTCCTGTCACCACAGGATCCATTTATTCTTACAGCTCCT
TCCAGGATCATTACCATGAC
TTCAGTGTCCCCTGTCATTCCGCTACCACACC
mm4_intergenic_Gm26913|Gm4076_chr13_84861963 CCTCCTTTAGTTTGCGCCTCAAATCTTTAGAATATGTTTTCCCTACTTGAGGAAGTTATTAGATGGTGAGGTTTCTGGAG
GG
CACCAGAGTCCTTGTGTGGTTATCAATTGCCCTGAACTGCTCCTTT
mm4_intergenic_Gm11115|Gm7714_chr5_88254667 GCACGCACTTGCAGTCATTATAACTACTATACCATCTACTGTGTTATTTGCATACAATTAATCCTTCCAGAAACCTAAGC
TTGTT
CCAGGAAGCCAACTGGTTATCAGG
mm3_intergenic_Gm23558|Galc_chr12_97974205 ACGAATGCAACGGTAGTTTGCGAATTTATTTTCCGCAGATGAAATCGATATGTTTCTGGAAAGGACTTTACCCTGCGTTT
ATTCACTGGGGAGCATTTGTGTCCT
mm4_intergenic_Gm23526|Grik4_chr9_42933361 TATGGCAAGCACTCCAAGGGAGAAAGGGCATGGGGCTGTTTCTGGAAGGGACTAGGATAAGATAGGGGAAGAGCATCAGG
AAAGG
mm4_intergenic_Gm7729|Gm24246_chr18_28156554 CCAGTTCCCTTGTCTCTGCCTTGGAACCATTCAGAAACAGAACTTTGTCTTGGAAAGGAAGTACGATTTTCAGAGACATT
AGACTCATTTGGAGCAGGGGCCCAGTTTCTTCAGCCGAGCACCTT
mm4_intron_Abhd12b_chr12_70163687 GTGAAGGTGTTCTGGCAAACACATTCTCGTTAAATAAGCAGTTCCCATATAGAATGACTTGGGTCTGTTTCTGAACGGGA
GGGAATAATAATTCTTCTGAAATGTCCATCTCAATTTCAATGAAGCCACTGGTTTCT
mm3_intergenic_mmu-mir-6393|Zdhhc25_chr15_88128812 GGGTTCACCTCCCTGTGTTCAATGCCTTCCAATAACATCACCATGTCATGCCAGCAAGGGTTTAATTAACAGGTCAGGTC
AGAGCTCT
mm4_intergenic_Gm22483|Prelid2_chr18_41099430 TCCCATAGGAATTCAAGACAAGCTAAATGCTAAAAAATTTTCTGTTTATTGTGTAAGGTAAAGAATGATGTACCACAGCC
ATGTTTCTGTTTCTGAATGG
GTGGTAGTAGCCAAGAGAGGCAGTAGATACTCAGCA
mm4_intergenic_Gm22254|Gm8993_chr6_74532236 TCCACATTCAAGGAACTCTCTCCTAATTGTTCTCAAGAGACATTATTATGTTTCTGTCAGGTTAATAGTTTAGAGTATAC
AGTTCTGCTTCTTGTTCTGT
mm4_intergenic_Il1rapl2|Gm25393_chrX_137767947 CCTAGTGCCAGAGAGCCCTATACCACAGACATGGTTATTAATCTGGTTGGTTTTGCTCTGATTCATGACTCTACCTTTAG
CCCC
mm4_intergenic_4930435H24Rik|Qdpr_chr5_45352631 ATCTTATGACCCTACTATGTGCATATTTAATTATAAATGCTCCAGCCCTTCCAAAAACATAAGGAAGTCCACAATGTTAT
CTTCTCAGACAACATTAGTCACTCTTCAGCTGAAAGGGCA
mm4_intergenic_Gm5432|Lpin1_chr12_16277297 CTGACACCCTGCACAGCATTGCTTGGCCCAGCACCAGGTCCCCTTCCTGGAACAAAAGCATGTCCTGAGCAGCCCTCGCT
GTCAGTGTCAGGCCTGGTGAACTT
mm4_intergenic_Gm13018|Prdm2_chr4_142902302 TCTCCAGTGTGAGAACGGAATACCACAAATATAATGGAACATATGGTTCTGTTTCAGGAGGGTTGTGATCGGTCTGGAGA
CGCGTTCACAT
mm4_intergenic_Smr3a|Smr2_chr5_88075525 AGCTGGGCATCACTATATTGCATGTACTTTTCCTGTCATTATGACTACTGTACCATCTACTGTGATAATTGCATACAATT
AATCCTTCGAGAAACCTAACCTTGTTCCAGGAGACAAATTGGTTATTAGGG
mm4_intergenic_Irx1|Rpl9-ps4_chr13_72217015 CTCTACAGGATGCTGGCCAGAAATAAAAAGTTCTGCCAAGTCAAATTAGGAAAGGAGATGCAGCTGCCAGGGTGATGTTC
CTGGAGGG
TCTTAGAGGGTTGCCTTCATAATTCCCT
mm4_intergenic_Rdh14|Pgk1-rs7_chr12_10542962 CTCGTACAGGGGCCAAAGATATCCCAAGGGATGAGTTAGAAATGCTTATGTTTTTGCATGGTTGAATGAATCTAGAGTAG
AATATCCCAAGAGAAACAGGTGGTTTGGAGATAGTTTCTAAGAGGGAAACCTGGTCCTGA
mm4_intron_Inppl1_chr7_101828916 CGTGAAGTCCACAGCTCCAAAGACCCAGGCATCCTCCACACCCCAGAACCATCCAGGAACATAGCCAGGCCCTCACTCAC
TAAATCGAAGGAAGACCTTATGCTTCTCCCGCTCC
mm4_intergenic_Gm23067|Gm26072_chr5_70240084 AGTTTGGTTGGTCCCTGTGCACAAACCCTCCACAAACATAACCCTGAAGCATTTTAATCTGTCCTGAGGAATTTGTTTCT
ACCT
mm4_intron_Egfr_chr11_16870898 TAGCAGCCCATACACAGCAGGACATGAGGATGTTTGTGGAAGGTTAGGAGCACCTGCTGTAGCCTTCAACACCTTCTTTT
CATTGCCTCTTCCTCAGGAAACTACGTGGTGACAGATCATGGCT
mm4_intergenic_Gm22813|Mpped2_chr2_106826913 CCAAATCCCAGCTCCTCTGTTTATAGAGAATTCCTGTTTTACCTGACCAATAAGTCATCCAGTGTATAGAGAGTATGTGT
GACTTATTTCACGCCCTCCTGAACCATAACAATGACTTGCCCTGATAGCTCTTGTAACA
mm4_intergenic_Gm25169|Efnb2_chr8_8031146 TGGTTGCACAGAGTTACGTCATAATTTTTTTAAAAATCCAATTAATACAGCTGCCATAGAAAAACATAAGCATGTCACTG
TCTCATTTAATAGTAACATTCTACTCAGTGATAACAAATCAGTTTATGCCTCCAGCCT
mm4_intergenic_Slc22a23|Pxdc1_chr13_34499968 TGAGCTTGGTCCTCAGATAGCTTCCTAAAGGTCTTTAATTCTCTTGTGAATCCATTCACAGAGTATATAATACTAGACAT
GGATATTCTCCTGGAGGG
CAGCTTTGGTCTCACAAGAAGT
mm4_intergenic_Slc1a1/AC163993.1|AC163993.1/Slc1a1_chr19_28896779 TCACATTGCTCTCCACAGCCCCCACCAGAAACAGAACCACCTCTATTTATGAGGTGCCTCTGGAGCT
mm4_intergenic_Actrt2|Gm13113_chr4_154818892 TCAGACATACGGCCTGATGCCTTCGTGCCCATGTGACCTTGATGTGCCTAACTGCCCTGCAGGAACATAACCCTGACCCT
GATGGGGACCCTAAGGA
mm4_intron_Stox1_chr10_62713802 ATTACACCAAAACACGGCGGCCGTATTTTATACAAACACGACCCATGCACCATATACAAACATAACCATGGCAATGCTTG
TTGCACAAGGCCTCGACCACCATAACAGT
mm4_exon_Macf1_chr4_123350937 GAGGCAGTGGTGGTCTTCTTAGACATGGTTGGGATTTTGGACGGTTTGGTGAGCCCTCGTCTGGAGCTGCCCTGGCCCCC
GGCAGCACTGCTCTCTGAAGTGTCTGAGCAAGCCGA
mm4_intron_Zdhhc24_chr19_4880962 TCTGATTTGATGGGGCGAGGGGAGCCAGTGAAAGTTACAGGCAGGGAAGTCCTTTCCTTTGGAAATTGGGAGGAGACTTG
GTTGTGTTACTGGCTGG
CTTCTGATGGTGTGAGTACTCCTCTTTCCTCTGACACCTCCGT
mm4_intergenic_Sh3rf1|Cbr4_chr8_61471945 AGGGGCAGAGAGATACGTAGAGAGATGGCTCTGTTCCTGGAAGGTAGCCAATCACCAGCTGCAGTTCCCACAGGGGTGCC
ACTTACTT
mm3_intergenic_Zfyve20|Trh_chr6_92220552 GTCTTGGTCATGCTCACCCTAATTTCTCCCCTAATTCTTTCCAGAAACACCACCATCTCCTCCCCAAGGTCTTCATGTGC
mm4_intergenic_Sprr1b|Sprr3_chr3_92439617 GAGACTGGCAAGGTGAGCATCCTTTCTCAAACTTAACCATGTCTTAAAGGATGAAGCAGCTGTGTTAACACGGGATTTCT
CCTTCAGAGAAAGAAGGAAAACTTTCTCTTATAAGCGAGGACAGCACTAACC
mm3_exon_Vta1_chr10_14684124 AAAGGCTGGCAGTGTAGAAGGACTTGATCATGTTTCTGGAAGAGAAAGGGTATCCTAATTTAAATTAAANNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNATACACTGAAATGTAGTCATGTACCCGT
mm4_intron_Bicc1_chr10_71077810 CAGCTCCTCCATGCCTCATTACTCTGCAGAAGAGCCATGGTTCTGTTTCTGTCTGGTCCAAACACTGACATGTTCTTTCC
AGTAAACTTAGACATTTCCCTTTTATTACATTGATTTTTCCAACATGAGCCTTCAAAGG
mm3_intron_Nos1_chr5_117787931 TGGAGAAAGAGAAGCTCAGCCTGGGACTTGGCTATGTTTCTGGCCAGTGAGTCCTCTCAACACTGATTCCTGCCCC
mm4_intron_Jak1_chr4_101171424 CTGCTTCTGGCCACCCAATACCTGCCATCCACAAAAACAACCATGTTTACAATGGGATCAGGCAATGTAATATTAGGGGT
CACTGAGCAAGAAGG
mm4_intergenic_Phlda1|4933440J02Rik_chr10_111569682 TGGTACCCATCCCTTCCTGTACTCTGCATTTACCCATCCACAAAGGTAACCTTGTCTTTGTTTGAAAGCTCTCTCCCTAG
CTATCCTGATTCCATTAGCAGCTTGTTTCTAAGGAGGAACATTGACCTGTGC
mm4_intergenic_Lrrtm3|Ctnna3_chr10_64133056 ACAAGCTCTGCCTCATACCTATTTTTCAGACCTCCTTCCAAATCCCTAGGTCATCAAAGTCCTTGAGCATAGTTATCATG
TCTGACATGGTTATCTTTGTTCAGGGGAGAAGAGATTAGGCTGTGTTTGAGT
mm4_intergenic_Gm7665|Cdh2_chr18_16538650 ACAGTCACCACAATATGATGGATAAAGGAACTTATGATGGTTTTGTTTCTGGAGGACATATAAAGTTGCTACCAAATCTT
CCATTAAAAAAGCATATTTAACAATAACTAATCATTCATTGTTTGCTGCTTGC
mm4_intergenic_Gm5544|Notch2_chr3_97989087 GAGGGGAGGGGATGGAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTTCTTTC
TTTTTCAGCCCTCCAAAGAAATAAACATGTCTGTCTCCCTCCCTCCCTCTTGAT
mm3_intergenic_Gm25359|Gm21869_chrY_74915799 TTGTCAGGGGATGGTGAAGCATTTGTGAACAATACATAGTTAAAATGTGCTCGTGAACTTTTCATTAGATGATATGGGGA
AAATCATACTGGCATGGTTTTTTTTCTGGAAGATTCAATGACATGTCAACTCACACAGCT
mm3_intergenic_Gm23770|Gm21881_chrY_85779385 TTGTCAGGGGATGGTGAAGCATTTGTGAACAATACATAGTTAAAATGTGCTCGTGAACTTTTCATTAGATGATATGGGGA
AAATCATACTGGCATGGTTTTTTTTCTGGAAGATTCAATGACATGTCAACTCACACAGCT
mm3_intergenic_Gm23052|Gm20927_chrY_54933939 TTGTCAGGGGATGGTGAAGCATTTGTGAACAATACATAGTTAAAATGTGCTCGTGAACTTTTCATTAGATGATATGGGGA
AAATCATACTGGCATGGTTTTTTTTCTGGAAGATTCAATGACATGTCAACTCACACAGCT
mm4_intergenic_RP23-448C3.1/Trpm3|Trpm3_chr19_22484909 ACACAGACAGAAATGCACCTTTTCAACATTCTTTCCCACATAGTTTACAAGCATCAGTTGAAATGGTTATGTCTGTGCAG
GG
ACAGAAGGCTGTGGTAGGAAAAATTAGATCATGAGTGTGTAGGCAGATGTGGC
mm3_intergenic_Smr2|Gm11116_chr5_88109115 AGCATCCACTGTTGTCCTCATTTTGATTACCATATCACCTACTGTACTATTTGTATGAAATGGATTCTTCCAGAAACCTA
ACCTTGTT
CCAGAAGACAAATGGTCATTAGGA
mm4_intergenic_Gm25684|Gm9966_chr7_95480949 TGAGCAAGCAGTGAGATGAGCCATCCAGCAAAATAACCATGAGAGTGGCCAGGAACTTTGGNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGTAGGAGCAGCCATGTCT
mm3_exon_Cdr1_chrX_61183680 TCAACGTCTTCCACAAAATTCAGGTCTTCCAGAAAAATATCCAGGTCTTCCAACCAGTCTATGTCTTCCAGAAAAATCCA
GGTCTTCCAGCTCATC
mm4_intron_Dnah17_chr11_118033339 GCCTGATCACATGACTGCCTTGTTCAGGGTGGTATCAGAGGACGTGGTTATGGAGCTGGAGGGTGGGGACAGCATCACCA
CCTGTCTGTCA
mm4_intron_Tacc2_chr7_130725742 GCTTGCATAGTGATTGCAGGGGACATGCTTATGTGGATGGATGGGTTTGTGGCCTCATCTTTCCTTGCCT
mm4_intergenic_Gm22254|Gm8993_chr6_74803399 GCCCTACAGGAAACATTGCCATTATCTGTATTTCTTTTTATATTGTATATGTATGCCTGTGGACATGTTTATGTGTATGG
GTGG
TGGTGGTGGTAGATATATGTATATCAATACAAGTGCCAGCA
mm4_exon_Timp2_chr11_118303519 GAAGGGGTATGGCAGGGAACCTGGCCCGCCTGTCCCTCCAGACCCACTACCATGTCTAGGGAAAATAGGGGAGGGGGCAG
AATCACACTAGCGT

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.