CRISPOR

DNA break repair outcome predictions

Guide: AGCCGGCCCTCCGACTTCCC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CACGGTTGCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGA`	(Wild-type sequence)
221	`TGAGCCCGCGAGC-----------------------GTGGCGGGGGAGGCCAGGAGGAGG`	`23 bp deleted → frameshift`
198	`TGAGCCCGCGAGCCGGCCCTCCG--------------TGGCGGGGGAGGCCAGGAGGAGG`	`14 bp deleted → frameshift`
182	`TGAGCCCGCGAGCCGGCCCTCCGA----------GCGTGGCGGGGGAGGCCAGGAGGAGG`	`10 bp deleted → frameshift`
180	`TGAGCCCGCGAGCCGGC------------------------GGGGGAGGCCAGGAGGAGG`	`24 bp deleted → no frameshift`
179	`TGAGCCCGCGAGCCGG----------------GAGCGTGGCGGGGGAGGCCAGGAGGAGG`	`16 bp deleted → frameshift`
170	`TGAGCCCGCGAGCCGG-----------------AGCGTGGCGGGGGAGGCCAGGAGGAGG`	`17 bp deleted → frameshift`
155	`TGAGCCCGCGAGCCGG---------------------------GGGAGGCCAGGAGGAGG`	`27 bp deleted → no frameshift`
155	`TGAGCCCGCG---------------------------TGGCGGGGGAGGCCAGGAGGAGG`	`27 bp deleted → no frameshift`
154	`TGAGCCCGCGAGCCGGCCCTCCG-------------------GGGGAGGCCAGGAGGAGG`	`19 bp deleted → frameshift`
154	`TGAGCCCGCGAGCCGGC-------------------GTGGCGGGGGAGGCCAGGAGGAGG`	`19 bp deleted → frameshift`
153	`TGAGCCCGCGAGCCGGCC---------------------------------AGGAGGAGG`	`33 bp deleted → no frameshift`
148	`TGAGC-------------------------------GTGGCGGGGGAGGCCAGGAGGAGG`	`31 bp deleted → frameshift`
140	`TGAGCCCGCGAGCCGGCCCTCCGACTTCCC---------------------AGGAGGAGG`	`21 bp deleted → no frameshift`
133	`TGAGCCCGCGAGCCGGCCCTCCGACTTCC----------------------AGGAGGAGG`	`22 bp deleted → frameshift`
133	`TGAGCCCGCGAGCCG----------------------TGGCGGGGGAGGCCAGGAGGAGG`	`22 bp deleted → frameshift`
121	`TGAGCCCGCG--------------------------------GGGGAGGCCAGGAGGAGG`	`32 bp deleted → frameshift`
98	`TGAGCCCGCGAGCCGG----------------------------GGAGGCCAGGAGGAGG`	`28 bp deleted → frameshift`
98	`TGAGCCCGCGAGC----------------------------GGGGGAGGCCAGGAGGAGG`	`28 bp deleted → frameshift`
95	`TGAGCCCGCGAGCCGGCCCTCCGA-----------------------GGCCAGGAGGAGG`	`23 bp deleted → frameshift`
94	`TGAGCCCGCGAGCC-------------------------------------AGGAGGAGG`	`37 bp deleted → frameshift`
94	`TGAGCCCGCGAGCCGGCCCTCC-----------------------------AGGAGGAGG`	`29 bp deleted → frameshift`
94	`TGAGCCCGCGAGCCGG-----------------------------GAGGCCAGGAGGAGG`	`29 bp deleted → frameshift`
94	`TGAGCCCG-----------------------------TGGCGGGGGAGGCCAGGAGGAGG`	`29 bp deleted → frameshift`
89	`TGAGCCCGCGAGCCGG------------------------------AGGCCAGGAGGAGG`	`30 bp deleted → no frameshift`
82	`TGAGCCCGCGAG------------------------------------GCCAGGAGGAGG`	`36 bp deleted → no frameshift`
80	`TGAGCCCGCGAGCCGGCCC--------------------------------AGGAGGAGG`	`32 bp deleted → frameshift`
73	`TGAGCCCG----------------------------------GGGGAGGCCAGGAGGAGG`	`34 bp deleted → frameshift`
66	`TGAGC------------------------------------GGGGGAGGCCAGGAGGAGG`	`36 bp deleted → no frameshift`
63	`TGAGCCCGCGAGCCGGCCCTCCGA-------------------------------GGAGG`	`31 bp deleted → frameshift`
63	`TGAGCC---------------------------------------------AGGAGGAGG`	`45 bp deleted → no frameshift`
60	`TGAGCCCGCGAGCCGG--------------------------------------AGGAGG`	`38 bp deleted → frameshift`
55	`TGAGCCCGCGAG--------------------------------------------GAGG`	`44 bp deleted → frameshift`
55	`TGAG--------------------------------------------GCCAGGAGGAGG`	`44 bp deleted → frameshift`
54	`TGAGCCCGCGAGCCGGCCCTCCGA----------------------------------GG`	`34 bp deleted → frameshift`
51	`TGAGCCCGCGAGCCGG-----------------------------------------AGG`	`41 bp deleted → frameshift`
51	`TGAGCCCGC-----------------------------------------CAGGAGGAGG`	`41 bp deleted → frameshift`
47	`TGAGCCCGCGAG-----------------------------------------------G`	`47 bp deleted → frameshift`
47	`TG-------------------------------------GCGGGGGAGGCCAGGAGGAGG`	`37 bp deleted → frameshift`
44	`TGAGCCCGCGAGCCGG--------------------------------------------`	`44 bp deleted → frameshift`
44	`TGAGCCC--------------------------------------------AGGAGGAGG`	`44 bp deleted → frameshift`
38	`TGAGCCCGCGAG-----------------------------------------GAGGAGG`	`41 bp deleted → frameshift`
37	`TGAG----------------------------------------------------GAGG`	`52 bp deleted → frameshift`
32	`TGAG-------------------------------------------------------G`	`55 bp deleted → frameshift`
25	`TGAG-------------------------------------------------GAGGAGG`	`49 bp deleted → frameshift`

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