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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313687_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313687_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm3_intron_Nubpl_chr12_52151040_F | Primer3: not found at this Tm | N.d. | 0.48 |
| mm3_intron_Nubpl_chr12_52151040_R | Primer3: not found at this Tm | N.d. | 0.48 |
| mm3_intron_Nubpl_chr12_52150933_F | Primer3: not found at this Tm | N.d. | 0.48 |
| mm3_intron_Nubpl_chr12_52150933_R | Primer3: not found at this Tm | N.d. | 0.48 |
| mm3_intergenic_Tcerg1l|Mapk1ip1_chr7_138450037_F | TCGTCGGCAGCGTCCAGAGGGAGGTCTCTACGCT | 60.1 | 0.45 |
| mm3_intergenic_Tcerg1l|Mapk1ip1_chr7_138450037_R | GTCTCGTGGGCTCGGTGTGTCCTTCCATCTCTGGC | 59.3 | 0.45 |
| mm4_exon_Zdhhc14_chr17_5647928_F | Primer3: not found at this Tm | N.d. | 0.45 |
| mm4_exon_Zdhhc14_chr17_5647928_R | Primer3: not found at this Tm | N.d. | 0.45 |
| mm4_exon_Raver1_chr9_21081302_F | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_exon_Raver1_chr9_21081302_R | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_exon_Pygm_chr19_6393830_F | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_exon_Pygm_chr19_6393830_R | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intergenic_Aldh1a3|Asb7_chr7_66636869_F | TCGTCGGCAGCGTCTCATGAACCAAGTGTCAGTCC | 57.8 | 0.35 |
| mm4_intergenic_Aldh1a3|Asb7_chr7_66636869_R | GTCTCGTGGGCTCGGCCCCGCCTTTTGATTGTTGT | 59.3 | 0.35 |
| mm4_exon_Pygl_chr12_70195573_F | TCGTCGGCAGCGTCTGAACAGGTTCTCCTCCCCA | 60.1 | 0.34 |
| mm4_exon_Pygl_chr12_70195573_R | GTCTCGTGGGCTCGGCGGGACAGGCAACATGAAGT | 60.6 | 0.34 |
| mm4_exon_Slc35b3_chr13_38960421_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_exon_Slc35b3_chr13_38960421_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Fam46d|Gm732_chrX_107907409_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Fam46d|Gm732_chrX_107907409_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm7931|Slit2_chr5_47941110_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Gm7931|Slit2_chr5_47941110_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Ctnna3|Gm10118_chr10_63899089_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Ctnna3|Gm10118_chr10_63899089_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Khdrbs2_chr1_32403358_F | TCGTCGGCAGCGTCCAGGACAGCAGATGCAGGAA | 60.0 | 0.25 |
| mm4_intron_Khdrbs2_chr1_32403358_R | GTCTCGTGGGCTCGGGCTCATACTCCTGGGCCATC | 59.9 | 0.25 |
| mm4_intron_Lsamp_chr16_42095731_F | TCGTCGGCAGCGTCTGCATTCTTTTCTCTTTATTGGCA | 57.6 | 0.25 |
| mm4_intron_Lsamp_chr16_42095731_R | GTCTCGTGGGCTCGGAAAGGCGGGCCATCTACTTC | 60.1 | 0.25 |
| mm4_intron_Agap1_chr1_89876209_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Agap1_chr1_89876209_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Nrg2_chr18_36181468_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Nrg2_chr18_36181468_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm10247|Grin2a_chr16_9219363_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm10247|Grin2a_chr16_9219363_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Nubpl_chr12_52150862_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Nubpl_chr12_52150862_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_I0C0044D17Rik|Gm12851_chr4_98841424_F | TCGTCGGCAGCGTCTACTGGGATGCCTGCATGTG | 60.1 | 0.22 |
| mm4_intergenic_I0C0044D17Rik|Gm12851_chr4_98841424_R | GTCTCGTGGGCTCGGTGTGTAGAGTCACAGCATGGC | 60.3 | 0.22 |
| mm4_intergenic_Cacna2d3|Selk_chr14_29726910_F | TCGTCGGCAGCGTCGGTCACCACCTTTAGAGCCC | 60.0 | 0.21 |
| mm4_intergenic_Cacna2d3|Selk_chr14_29726910_R | GTCTCGTGGGCTCGGGGCTGCATTCCAGACTCAGT | 60.0 | 0.21 |
| mm4_exon_Man2a1_chr17_64600864_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_exon_Man2a1_chr17_64600864_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Sgk1_chr10_21931783_F | TCGTCGGCAGCGTCAGTGGAGAAGGTGGTGCTTG | 59.8 | 0.20 |
| mm4_intron_Sgk1_chr10_21931783_R | GTCTCGTGGGCTCGGCAGGTGAGTGCTGATCAGGT | 59.3 | 0.20 |
| mm4_intron_Mks1_chr11_87853452_F | TCGTCGGCAGCGTCTTAGATGCTCAGAGGCCCCT | 60.0 | 0.19 |
| mm4_intron_Mks1_chr11_87853452_R | GTCTCGTGGGCTCGGACTTGGGCAGGGTGAATCTG | 59.9 | 0.19 |
| mm4_intergenic_D430001F17Rik|Gm23210_chr16_92030884_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_D430001F17Rik|Gm23210_chr16_92030884_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm26197|Akap11_chr14_78316659_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm26197|Akap11_chr14_78316659_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Calca|Gm15500_chr7_114638432_F | TCGTCGGCAGCGTCCAGAAGTGGCCTTCCTGGG | 60.0 | 0.17 |
| mm4_intergenic_Calca|Gm15500_chr7_114638432_R | GTCTCGTGGGCTCGGAAGTGGCAATGACCCAGTGG | 60.5 | 0.17 |
| mm4_exon_Mier1_chr4_103119400_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_exon_Mier1_chr4_103119400_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Gcn1l1_chr5_115603056_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Gcn1l1_chr5_115603056_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm23207|Mapre2_chr18_23823379_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm23207|Mapre2_chr18_23823379_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_exon_Desi2_chr1_178187834_F | TCGTCGGCAGCGTCCTTAGTGCCCGGCTCGAG | 59.8 | 0.16 |
| mm3_exon_Desi2_chr1_178187834_R | GTCTCGTGGGCTCGGCATCCTTCTCCCCACGGC | 59.8 | 0.16 |
| mm4_exon_Gm355_chr2_128598443_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Gm355_chr2_128598443_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm25693|Gm12129_chr11_38047254_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm25693|Gm12129_chr11_38047254_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intergenic_Ube2e3|Gm14466_chr2_78990914_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intergenic_Ube2e3|Gm14466_chr2_78990914_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Slc38a7|Got2_chr8_95862563_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Slc38a7|Got2_chr8_95862563_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Zcchc6|Gas1_chr13_59982885_F | TCGTCGGCAGCGTCAGGAGGTGACCAGGGCTAAA | 60.1 | 0.16 |
| mm4_intergenic_Zcchc6|Gas1_chr13_59982885_R | GTCTCGTGGGCTCGGGGTCTCCTCTCCCAACTTCC | 59.0 | 0.16 |
| mm4_intergenic_Dmrt3|Dmrt2_chr19_25626176_F | TCGTCGGCAGCGTCCCTGGCAGGCATCTTAGCAT | 60.1 | 0.15 |
| mm4_intergenic_Dmrt3|Dmrt2_chr19_25626176_R | GTCTCGTGGGCTCGGCCAACAATCCCCAAGGCAGA | 60.2 | 0.15 |
| mm4_intron_Osbpl3_chr6_50364653_F | TCGTCGGCAGCGTCGAATACTGCACTGGCCACCT | 60.0 | 0.14 |
| mm4_intron_Osbpl3_chr6_50364653_R | GTCTCGTGGGCTCGGGCTAGATTCAGGGCCTGGTG | 60.1 | 0.14 |
| mm4_exon_Zswim8_chr14_20708016_F | TCGTCGGCAGCGTCGTTCATTTCCGAGGCCGAGA | 60.1 | 0.14 |
| mm4_exon_Zswim8_chr14_20708016_R | GTCTCGTGGGCTCGGCTCACCTCGCGTTCTGGTAC | 60.4 | 0.14 |
| mm4_exon_KCTD12/Gm26778/Kctd12/Mir5130_chr14_102982378_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_KCTD12/Gm26778/Kctd12/Mir5130_chr14_102982378_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Evl_chr12_108554973_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Evl_chr12_108554973_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Ythdc2_chr18_44828743_F | TCGTCGGCAGCGTCTCTCCTCAGGGCCATGTCC | 60.6 | 0.13 |
| mm4_exon_Ythdc2_chr18_44828743_R | GTCTCGTGGGCTCGGCAGGACCACACGAAGCTGG | 60.6 | 0.13 |
| mm4_exon_Plekhb2_chr1_34873735_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Plekhb2_chr1_34873735_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Foxk2_chr11_121298833_F | TCGTCGGCAGCGTCACAAGCTGTGGTCACTCAGG | 59.8 | 0.13 |
| mm4_exon_Foxk2_chr11_121298833_R | GTCTCGTGGGCTCGGGGGGTCTTGGGTGAATCAGG | 60.0 | 0.13 |
| mm4_exon_Guca1b_chr17_47385521_F | TCGTCGGCAGCGTCTTCTAAAAGCCGGGATGGGG | 59.7 | 0.12 |
| mm4_exon_Guca1b_chr17_47385521_R | GTCTCGTGGGCTCGGCTGGGACACTCCACGACAAA | 59.8 | 0.12 |
| mm3_intron_Sephs1_chr2_4882070_F | TCGTCGGCAGCGTCTTGGTGGGGCCGCTTTAAAA | 60.7 | 0.12 |
| mm3_intron_Sephs1_chr2_4882070_R | GTCTCGTGGGCTCGGAAGATTAACCCCTGCGTGGC | 60.6 | 0.12 |
| mm3_exon_Gspt1_chr16_11253659_F | TCGTCGGCAGCGTCGATATCCAGCCCGGCAACTT | 60.1 | 0.12 |
| mm3_exon_Gspt1_chr16_11253659_R | GTCTCGTGGGCTCGGGAATTCGTGCCGTCCTTCCT | 60.3 | 0.12 |
| mm4_exon_Zbtb7a_chr10_81138020_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Zbtb7a_chr10_81138020_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Casp3|Gm16675_chr8_46712237_F | TCGTCGGCAGCGTCGCCTTTTGCTCTGTTGTGCA | 59.8 | 0.11 |
| mm4_intergenic_Casp3|Gm16675_chr8_46712237_R | GTCTCGTGGGCTCGGGCACAAGGCACAGGAGAGAT | 60.0 | 0.11 |
| mm4_intergenic_Gm22073|Schip1_chr3_68565142_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm22073|Schip1_chr3_68565142_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm11340|Gm23340_chr13_24291956_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm11340|Gm23340_chr13_24291956_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Ccdc65_chr15_98720809_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Ccdc65_chr15_98720809_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_5330413P13Rik_chr2_131824877_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_5330413P13Rik_chr2_131824877_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Apbb2_chr5_66552370_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Apbb2_chr5_66552370_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm44|Gm14773_chrX_91047797_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm44|Gm14773_chrX_91047797_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm22118|Zswim6_chr13_107835900_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm22118|Zswim6_chr13_107835900_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Rhobtb3_chr13_75943546_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Rhobtb3_chr13_75943546_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm11449|Ptprt_chr2_161519439_F | TCGTCGGCAGCGTCGAGGCTGTTTCCTTAGGCGT | 60.0 | 0.09 |
| mm4_intergenic_Gm11449|Ptprt_chr2_161519439_R | GTCTCGTGGGCTCGGTGTATTTGGCATCAGGCTGT | 57.4 | 0.09 |
| mm3_exon_Tnfrsf25_chr4_152119515_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_exon_Tnfrsf25_chr4_152119515_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Fosl2_chr5_32152669_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Fosl2_chr5_32152669_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Dgkd_chr1_87853336_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Dgkd_chr1_87853336_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Phactr3_chr2_178142463_F | TCGTCGGCAGCGTCAGAGTGACCCCAGCTTCCTC | 60.9 | 0.08 |
| mm4_exon_Phactr3_chr2_178142463_R | GTCTCGTGGGCTCGGCACCTGCCTCGTGTCTCC | 59.7 | 0.08 |
| mm4_exon_Kras_chr6_145250172_F | TCGTCGGCAGCGTCCTCCGGGTCCGAAATGGC | 60.5 | 0.07 |
| mm4_exon_Kras_chr6_145250172_R | GTCTCGTGGGCTCGGCACTCCGGGCTCGATTCG | 60.2 | 0.07 |
| mm4_exon_Opa3_chr7_19228429_F | TCGTCGGCAGCGTCCGCCCAGTCAGCAAGGTT | 60.2 | 0.06 |
| mm4_exon_Opa3_chr7_19228429_R | GTCTCGTGGGCTCGGTAGAACAGCTTCGCCATGGG | 60.1 | 0.06 |
| mm4_intergenic_Zfp326|Gm25672_chr5_106045443_F | TCGTCGGCAGCGTCCACCATATGCACCAGAGCCT | 59.8 | 0.06 |
| mm4_intergenic_Zfp326|Gm25672_chr5_106045443_R | GTCTCGTGGGCTCGGGTTTAACCCCTGCAAAGCGG | 60.0 | 0.06 |
| mm4_exon_Unc45b_chr11_82911666_F | TCGTCGGCAGCGTCGCAAAAGGCTGACCACACAG | 59.9 | 0.06 |
| mm4_exon_Unc45b_chr11_82911666_R | GTCTCGTGGGCTCGGGAGCTGAAAATGCCGGTTCC | 59.8 | 0.06 |
| mm4_exon_Ttbk1_chr17_46487461_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Ttbk1_chr17_46487461_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Sertad4/Gm15867|Sertad4_chr1_192855116_F | TCGTCGGCAGCGTCCCGAGGAGACTGCTAGAGGC | 61.4 | 0.06 |
| mm4_intergenic_Sertad4/Gm15867|Sertad4_chr1_192855116_R | GTCTCGTGGGCTCGGCCGGAGCTGACACCTTGTG | 60.6 | 0.06 |
| mm4_exon_Grk4_chr5_34660734_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Grk4_chr5_34660734_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Ube2cbp|Dopey1_chr9_86467062_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Ube2cbp|Dopey1_chr9_86467062_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Gm20421/Hes5_chr4_154961385_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Gm20421/Hes5_chr4_154961385_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Ktn1|Gm6055_chr14_48019083_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Ktn1|Gm6055_chr14_48019083_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Nipbl|Gm2310_chr15_8444804_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Nipbl|Gm2310_chr15_8444804_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Ubn2_chr6_38433963_F | TCGTCGGCAGCGTCGCGGCCTGCTTCTATTTATGTG | 60.0 | 0.05 |
| mm4_exon_Ubn2_chr6_38433963_R | GTCTCGTGGGCTCGGTCCCCGCTACTACGTTCTGA | 60.0 | 0.05 |
| mm4_exon_Pde10a_chr17_8801883_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Pde10a_chr17_8801883_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Liph_chr16_21955702_F | TCGTCGGCAGCGTCTCCAAACCCTTCACAGCCTG | 60.1 | 0.05 |
| mm4_exon_Liph_chr16_21955702_R | GTCTCGTGGGCTCGGAAATGGTCCCTGTGCCCAAT | 59.8 | 0.05 |
| mm4_intergenic_Gtf2a1|Ston2_chr12_91634909_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gtf2a1|Ston2_chr12_91634909_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm22216|Cldn23_chr8_35802303_F | TCGTCGGCAGCGTCGGTGATCTCATGTGGCCCAA | 60.0 | 0.04 |
| mm3_intergenic_Gm22216|Cldn23_chr8_35802303_R | GTCTCGTGGGCTCGGCCCTGCACTTACATCCAGCT | 59.7 | 0.04 |
| mm4_intergenic_AK129341|1700128F08Rik_chr9_8134348_F | TCGTCGGCAGCGTCTGCGGCAATCCTGGCTTC | 60.7 | 0.04 |
| mm4_intergenic_AK129341|1700128F08Rik_chr9_8134348_R | GTCTCGTGGGCTCGGCGGTTGGTTGTCAAGATGGC | 59.7 | 0.04 |
| mm4_intergenic_Jazf1|Gm25818_chr6_53287901_F | TCGTCGGCAGCGTCTGGAACCCTTCTGAAGTGTGG | 59.8 | 0.04 |
| mm4_intergenic_Jazf1|Gm25818_chr6_53287901_R | GTCTCGTGGGCTCGGCGCTGCATCACTGAGAAACG | 59.9 | 0.04 |
| mm4_exon_Shank1_chr7_44336014_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Shank1_chr7_44336014_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Ncoa1|Gm3625_chr12_4477186_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Ncoa1|Gm3625_chr12_4477186_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Zfp618_chr4_62965578_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Zfp618_chr4_62965578_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Tmem55b_chr14_50930811_F | TCGTCGGCAGCGTCCGACTCCCGCTCGGTCAG | 62.1 | 0.04 |
| mm4_exon_Tmem55b_chr14_50930811_R | GTCTCGTGGGCTCGGCCAGCCTGTCACGTGAGC | 60.7 | 0.04 |
| mm4_exon_Scx_chr15_76458081_F | TCGTCGGCAGCGTCCTTTCTTCCACAGCGGTCGT | 60.6 | 0.04 |
| mm4_exon_Scx_chr15_76458081_R | GTCTCGTGGGCTCGGAGATCTGTTTGGGCTGGGTG | 59.9 | 0.04 |
| mm4_exon_Lbx1_chr19_45235441_F | TCGTCGGCAGCGTCGGGAGGAAAGCGCTGACG | 60.8 | 0.04 |
| mm4_exon_Lbx1_chr19_45235441_R | GTCTCGTGGGCTCGGTTAAATAGCACCGGGGCTGG | 60.1 | 0.04 |
| mm4_exon_Cxxc4/Gm26691/Mir1895_chr3_134240103_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Cxxc4/Gm26691/Mir1895_chr3_134240103_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm11655|Prkca_chr11_108267780_F | TCGTCGGCAGCGTCATGTTCACTGGAGCCAAGGC | 60.6 | 0.03 |
| mm4_intergenic_Gm11655|Prkca_chr11_108267780_R | GTCTCGTGGGCTCGGTTTGCTAAGCCAGGTGTGGT | 59.8 | 0.03 |
| mm4_exon_Btbd11_chr10_85388310_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Btbd11_chr10_85388310_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Tlk1|Gm13633_chr2_70825781_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Tlk1|Gm13633_chr2_70825781_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Tspan5_chr3_138742279_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Tspan5_chr3_138742279_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm15494|Eps8l1_chr7_4450243_F | TCGTCGGCAGCGTCCACAGAGCCTGGGCAAAGAT | 60.3 | 0.02 |
| mm4_intergenic_Gm15494|Eps8l1_chr7_4450243_R | GTCTCGTGGGCTCGGTCTGCCTTCTGCTTCTTGCC | 60.6 | 0.02 |
| mm4_exon_R3hdm4/Kiss1r_chr10_79917119_F | TCGTCGGCAGCGTCAGGACAAAGTCTGAGCCACC | 59.6 | 0.02 |
| mm4_exon_R3hdm4/Kiss1r_chr10_79917119_R | GTCTCGTGGGCTCGGCTGTCACCCTGGGACTGTTC | 59.9 | 0.02 |
| mm3_intron_Gcnt4_chr13_96944697_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Gcnt4_chr13_96944697_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Rnf208/2310002J15Rik_chr2_25242299_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Rnf208/2310002J15Rik_chr2_25242299_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Arid1a_chr4_133753345_F | TCGTCGGCAGCGTCGAGTTCTTCAGGTCCGGCTC | 60.1 | 0.02 |
| mm4_exon_Arid1a_chr4_133753345_R | GTCTCGTGGGCTCGGGGGAAAGGAGCTGCAGGAC | 60.3 | 0.02 |
| mm4_intron_Prkd3_chr17_79020537_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Prkd3_chr17_79020537_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Car10_chr11_93099889_F | TCGTCGGCAGCGTCTCTATGTCTTCTTGGGGCGC | 59.8 | 0.01 |
| mm4_exon_Car10_chr11_93099889_R | GTCTCGTGGGCTCGGCCTCTCACCAGTGGCAGAAA | 59.6 | 0.01 |
| mm4_exon_Nrsn1_chr13_25269936_F | TCGTCGGCAGCGTCCCTAGCTGCAGATCTCTCGC | 60.0 | 0.01 |
| mm4_exon_Nrsn1_chr13_25269936_R | GTCTCGTGGGCTCGGCTGTCAATCACGTGCAGCTG | 59.8 | 0.01 |
| mm4_exon_Kcnc2_chr10_112271870_F | TCGTCGGCAGCGTCTGCCTCCTCTGAACCTCAGG | 60.9 | 0.01 |
| mm4_exon_Kcnc2_chr10_112271870_R | GTCTCGTGGGCTCGGGGGACAGGGGACAAGGGA | 60.2 | 0.01 |
| mm4_intergenic_Gm25327|Ninj2_chr6_120153817_F | TCGTCGGCAGCGTCCCCAACACTGGCAGTTCAGA | 60.1 | 0.01 |
| mm4_intergenic_Gm25327|Ninj2_chr6_120153817_R | GTCTCGTGGGCTCGGCTGGAGAGTGGGCATCTTCC | 59.8 | 0.01 |
| mm4_intron_Srgap3_chr6_112759563_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Srgap3_chr6_112759563_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Rad54l2|Gm22384_chr9_106789752_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Rad54l2|Gm22384_chr9_106789752_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Pitpna_chr11_75588183_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Pitpna_chr11_75588183_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Nrip1_chr16_76372990_F | TCGTCGGCAGCGTCTCCCTCCTTCCCAGGTTAGC | 60.6 | 0.01 |
| mm4_exon_Nrip1_chr16_76372990_R | GTCTCGTGGGCTCGGAGACATTGCAGCAGAGCCC | 60.3 | 0.01 |
| mm4_exon_Zfp687_chr3_95015392_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Zfp687_chr3_95015392_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Memo1_chr17_74294516_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Memo1_chr17_74294516_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Bcl11b|Setd3_chr12_108006177_F | TCGTCGGCAGCGTCAGCTTAGGGGTCCAGGAGTC | 60.3 | 0.00 |
| mm4_intergenic_Bcl11b|Setd3_chr12_108006177_R | GTCTCGTGGGCTCGGCTGGGGTCAGTCCGGACT | 60.2 | 0.00 |
| mm4_exon_Rnf215_chr11_4135320_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Rnf215_chr11_4135320_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Flrt1|Macrod1_chr19_7165280_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Flrt1|Macrod1_chr19_7165280_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm20204|Cmip_chr8_117256848_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm20204|Cmip_chr8_117256848_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pvrl3_chr16_46496999_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pvrl3_chr16_46496999_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Serpine2|Gm5529_chr1_79858792_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Serpine2|Gm5529_chr1_79858792_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rpl12|Slc2a8_chr2_32966997_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rpl12|Slc2a8_chr2_32966997_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Wfs1_chr5_36988907_F | TCGTCGGCAGCGTCACCCTGAAGAACTTACCGGC | 59.6 | 0.00 |
| mm4_exon_Wfs1_chr5_36988907_R | GTCTCGTGGGCTCGGCTGAATGTAGGGCCGCGC | 61.2 | 0.00 |
| mm4_exon_Mtmr2_chr9_13749279_F | TCGTCGGCAGCGTCGCTCCTCCCTCTGCATCTC | 59.2 | 0.00 |
| mm4_exon_Mtmr2_chr9_13749279_R | GTCTCGTGGGCTCGGCTTCTCCATGGCGCGAGAAA | 60.7 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm3_intergenic_Tcerg1l|Mapk1ip1_chr7_138450037 |
CAGAGGGAGGTCTCTACGCTAGCCCCTGGACAGCAGCAGCAAGAGCCTCAGATGATACAGAAGGTGGAGACCCAGGAGAT GGCAGGGGCTTTTGAACAGCCAGAGATGGAAGGACACA |
| mm4_intergenic_Aldh1a3|Asb7_chr7_66636869 |
TCATGAACCAAGTGTCAGTCCCCTCTACCATCTCCTCTGTCTCCCTTTTCTAGAAATGTCTGAGCCCAAATAACAACAAA CCTGAACACCTTCAGATGACAACAATCAAAAGGCGGGG |
| mm4_exon_Pygl_chr12_70195573 |
TGAACAGGTTCTCCTCCCCAGCTTCCTCTGCCATCTCCACATTGGCCCCATCCATAGTCCCGATGGTCAGGGCCCCGTTC AGCATGAACTTCATGTTGCCTGTCCCG |
| mm4_intron_Khdrbs2_chr1_32403358 |
CAGGACAGCAGATGCAGGAAGAGAAGCTGGGGACTGTGGGGATGGCAGGGGAGAGGTTGTCCCTAGGAGAGCACAATACG GACATGGGATACAGTTATAGTAACACAGATGGCCCAGGAGTATGAGC |
| mm4_intron_Lsamp_chr16_42095731 |
TGCATTCTTTTCTCTTTATTGGCATTTGCAAATATCTTTAAATCCAATGCCATCTCCACCGTCTCCAGATTCATCTTTCA CACATTGAGAAAGTAGTAGTGGTCTCCACAGACAAGTTCAGAAGTAGATGGCCCGCCTTT |
| mm4_intergenic_I0C0044D17Rik|Gm12851_chr4_98841424 |
TACTGGGATGCCTGCATGTGTTAATTTAGACCACCCCTGAGATGGCAGAGGATGATTGACAGCCATGCTGTGACTCTACA CA |
| mm4_intergenic_Cacna2d3|Selk_chr14_29726910 |
GGTCACCACCTTTAGAGCCCGCCCTGTCATGTGTTGTTTCTAGCCCCGTGTGCTACCTGGGCTGGGGAGACTGCAGTGAT GGCAGAGGTGCTCTGCTGCCCAGAGCAGTTCTACTGAGTCTGGAATGCAGCC |
| mm4_intron_Sgk1_chr10_21931783 |
AGTGGAGAAGGTGGTGCTTGCTAGGCAAGTCATCAAGTCATTCCCCTGCCATCTCCTGTGTGGCCTGGCCTAAGGGAATC CTTCGTTTACCTGATCAGCACTCACCTG |
| mm4_intron_Mks1_chr11_87853452 |
TTAGATGCTCAGAGGCCCCTACTTCCGCTTACAATCCCAACTACTTCCTCTGCCATCTCTGCGCGCGTCTTGTTATCAGG ATCTTTTTCCACTGAAAGCTTCCAAGTACACCTTTCAGATTCACCCTGCCCAAGT |
| mm4_intergenic_Calca|Gm15500_chr7_114638432 |
CAGAAGTGGCCTTCCTGGGCTTCCAGCAGTTTAGTTCTGAGACCTGGACTAAATTTTCGTGAGCAATTATGAGACAAGTT GGAGACAAGTTGGCGAGGGCTGAGATGACAGTGGTGAGCCACTGGGTCATTGCCACTT |
| mm3_exon_Desi2_chr1_178187834 |
CTTAGTGCCCGGCTCGAGCCCTGCCTGAGGCGCCCGCGGGGGTGGCAGCGGCCTCCGCCCCGCGGAGACCGAGCGGCTGG AGGACGAGGCGGCGGCCGTGGGGAGAAGGATG |
| mm4_intergenic_Zcchc6|Gas1_chr13_59982885 |
AGGAGGTGACCAGGGCTAAATCTCAAAGGAAGAAACAAGGGGTTTTGGTGACAGCTTAGATGGCAGGGGAAGTAGTACAT GAGCTAAAAACACTTCTGGGTCCCAGGTTTTATTTCCAAGGAAGTTGGGAGAGGAGACC |
| mm4_intergenic_Dmrt3|Dmrt2_chr19_25626176 |
CCTGGCAGGCATCTTAGCATGAAGGCAACTGTGGAGATGCCAGGGGCCTCTGTCCTCCCTAGCAGGCTGTTGGCTGGGTG GCTGCTTCTTTTGGCTTCTGCCTTGGGGATTGTTGG |
| mm4_intron_Osbpl3_chr6_50364653 |
GAATACTGCACTGGCCACCTGTGGCCTCTGCCATCTGCGGAGTCGGCTAACCACACCCTGAGAGTATTCCAAGAGAAAGA GTGCCCAAGCATGTAGAGACATTTCCTCTCATCACCAGGCCCTGAATCTAGC |
| mm4_exon_Zswim8_chr14_20708016 |
GTTCATTTCCGAGGCCGAGAGCCTTTGCCAGAACTGGCGAGGATGGCGCAAACAGTCAGCGGGGCCCAATTCCCCCACTG GAGGCGGTGGCGGAGGTGGCAGTGGCGGTACCAGAACGCGAGGTGAG |
| mm4_exon_Ythdc2_chr18_44828743 |
TCTCCTCAGGGCCATGTCCAGGCCGAGCAGCGTCTCGCCGCGTCCGCCGGCTCCTANNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCAGCTTCGTGTGGTCCTG |
| mm4_exon_Foxk2_chr11_121298833 |
ACAAGCTGTGGTCACTCAGGCTGCGGTGCTGGCTCCTCCTAACCCTGAACCACAAGAGAATGGCGACCACAGAGAAGTCA GAGGTGAGCAAGGAAAAAAATCTCCCATTAGTTTCTCATTCCTGATTCACCCAAGACCCC |
| mm4_exon_Guca1b_chr17_47385521 |
TTCTAAAAGCCGGGATGGGGCAGCAGCTGAGCTGGGAAGAGGCGGAGGCGGCCGGGGAGATGGATGTGGCGGAGTTGCAG GAGTGGTACAAGAAGTTTGTCGTGGAGTGTCCCAG |
| mm3_intron_Sephs1_chr2_4882070 |
TTGGTGGGGCCGCTTTAAAAAAGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGCGGGGCCGG GGTTGGGAGGGAGGAGCGGAGGGGGAGGGCGGCGGCGGCCACGCAGGGGTTAATCTT |
| mm3_exon_Gspt1_chr16_11253659 |
GATATCCAGCCCGGCAACTTACCCGAAGGGCCTCCCGCGCCGCTGCCTGCTCCGTGGTCGCCTCCGGCTGCGCCAGCTGG GGATAGCGGCGGCTGGGCCGGACCCCGCAGGAAGGACGGCACGAATTC |
| mm4_intergenic_Casp3|Gm16675_chr8_46712237 |
GCCTTTTGCTCTGTTGTGCAATATTAGGGGGTGGCCATATTTCCTCTGCAGCTCTGTCTTTTTCATCTTTAGGTCGAGCA CGGAGATGGCGGTGGCCATCTCTCCTGTGCCTTGTGC |
| mm4_intergenic_Gm11449|Ptprt_chr2_161519439 |
GAGGCTGTTTCCTTAGGCGTATATAGGAATGTCTGGTTTTGATATACCCAGGCTAGCTTAGCCTCCAGTTCAAGGGGACT GCAGAGATGTCAGGGGCAAGGCCCATAAATATACAGCCTGATGCCAAATACA |
| mm4_exon_Phactr3_chr2_178142463 |
AGAGTGACCCCAGCTTCCTCAGCGACTCCTCAGCCACCTCCACGGACGCCGGGGAGAACCCAGGTACTGGGCCCGGCGGG GGCGGCCAGGCGGGGTGGGGGAGACACGAGGCAGGTG |
| mm4_exon_Kras_chr6_145250172 |
CTCCGGGTCCGAAATGGCGCGGGAGCGGGACTCCGGGCCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCCGAATCGAGCCCGGAGTG |
| mm4_exon_Opa3_chr7_19228429 |
CGCCCAGTCAGCAAGGTTGCGCGTGCCCCAGGGCGGCCGCCGAGATGGTGGTGGGCGCGTTCCCCATGGCGAAGCTGTTC TA |
| mm4_intergenic_Zfp326|Gm25672_chr5_106045443 |
CACCATATGCACCAGAGCCTCTCCCTCGCCACTGCCACCGCCGCTGCCGCCTCACAACCTGAAGTGTGTATTTTCCTTCC ATTTTTGCCAAGTGACCCGCTTTGCAGGGGTTAAAC |
| mm4_exon_Unc45b_chr11_82911666 |
GCAAAAGGCTGACCACACAGGGCTGTTGGTGTCCCCGCAGATGGCAGAGGCTGAAGCGGCACAGCTGAAGGAGGAGGGGA ACCGGCATTTTCAGCTC |
| mm4_intergenic_Sertad4/Gm15867|Sertad4_chr1_192855116 |
CCGAGGAGACTGCTAGAGGCGGGGGCGTGGGCCTGGCAGCCCGCGGGGAGGTGAGCCGAGGACAGCGCCGCCCGCAGCCA GGAGCAGGGGCGGGCGCGGAGGTGGCGGGGGACACAAGGTGTCAGCTCCGG |
| mm4_exon_Ubn2_chr6_38433963 |
GCGGCCTGCTTCTATTTATGTGGGGGATCCAACATGGCGGCTGCGGCGACCCTGGCGATGGCGGTGGAGCCCATCAGAAC GTAGTAGCGGGGA |
| mm4_exon_Liph_chr16_21955702 |
TCCAAACCCTTCACAGCCTGGCCCTCCCCACCCACAGTCCCTGTGAGGTAGTCTGGTTCATTCTCCATTCGGTTCCCGCC GAGATGGCAGAGGATTGGGCACAGGGACCATTT |
| mm3_intergenic_Gm22216|Cldn23_chr8_35802303 |
GGTGATCTCATGTGGCCCAAGGCAGTGGGCTTGTGACAGTGGAGACCAGTGTGTGCCTGACTTCTGGCACTGTGATGGAC AGAGCGACTGCAGAGATGGCAGTGACGAAGCTGGATGTAAGTGCAGGG |
| mm4_intergenic_AK129341|1700128F08Rik_chr9_8134348 |
TGCGGCAATCCTGGCTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNCCCGCCGCAGCCATCTTGACAACCAACCG |
| mm4_intergenic_Jazf1|Gm25818_chr6_53287901 |
TGGAACCCTTCTGAAGTGTGGTCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACAG ACCAGGAACCTGGAGCCTCCTCGCATCCCGTTTCTCAGTGATGCAGCG |
| mm4_exon_Tmem55b_chr14_50930811 |
CGACTCCCGCTCGGTCAGCGACCAGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTCCCTAAGGCG CCTGCGCGCCGCGTTCGCCCGCAGCCAGTGCCGCCCCGCTCACGTGACAGGCTGG |
| mm4_exon_Scx_chr15_76458081 |
CTTTCTTCCACAGCGGTCGTGCGGGCAGCCCACTGCCACCGCCGCCGCCGCCACCACCACTGGCCAGAGACGGCGGCGAG AACACCCAGCCCAAACAGATCT |
| mm4_exon_Lbx1_chr19_45235441 |
GGGAGGAAAGCGCTGACGCGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGTTTCCAGCAAGCCCGGG CCGGAGCCGGGAAGCGCCGCGCCGGGCTCCAGTTTCGCCAGCCCCGGTGCTATTTAA |
| mm4_intergenic_Gm11655|Prkca_chr11_108267780 |
ATGTTCACTGGAGCCAAGGCTAGCTTTTACTCCTCTGCCAGCTCCCCGCTCCCCCGGAGGTGGGGTTGCAGGCTGCACCA CACCTGGCTTAGCAAA |
| mm4_intergenic_Gm15494|Eps8l1_chr7_4450243 |
CACAGAGCCTGGGCAAAGATAGGCTGGAGGCAACCCTGGAGAGGGCAGAGGAGGCCTGGGCAGGGAGGCAGGACACACCG CTGGCCAGGAAGGGTGCTTCCAAATGTGAGAAGAACTAGGCAAGAAGCAGAAGGCAGA |
| mm4_exon_R3hdm4/Kiss1r_chr10_79917119 |
AGGACAAAGTCTGAGCCACCCGGGAGGAGACGGTGGGGCACACCAGCCCCGGGGCGACTGCGGAGATGCGAAAGGCTCCT TGGGGCGCAGATGCGGGAGGGGTATGCGAGCCCCGCGAACAGTCCCAGGGTGACAG |
| mm4_exon_Arid1a_chr4_133753345 |
GAGTTCTTCAGGTCCGGCTCCGCGCCGGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCATTGCTCTCGGC CCCGTCCTGCAGCTCCTTTCCC |
| mm4_exon_Car10_chr11_93099889 |
TCTATGTCTTCTTGGGGCGCCCGGCGGATCGGGGTCTCGGTTTTGCAGGAAAAGCCCAGTGCTGTTGGCTTCAGGTGGCT GCCGCCGCCGCCACCGCGGTCGCCGCAAGTGCCATTTCTGCCACTGGTGAGAGG |
| mm4_exon_Nrsn1_chr13_25269936 |
CCTAGCTGCAGATCTCTCGCTGGGGTCCCATCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNTCTCCAAGCTCAGCTGCACGTGATTGACAG |
| mm4_exon_Kcnc2_chr10_112271870 |
TGCCTCCTCTGAACCTCAGGGCGACTGCCTGACTGCGGCCGGGGACAAGCTGCAACCGCTGCCCCCTCCGCTGTCTCCGC CGCCACGACCGCCTCCCTTGTCCCCTGTCCC |
| mm4_intergenic_Gm25327|Ninj2_chr6_120153817 |
CCCAACACTGGCAGTTCAGATCAAACCCTCCTGACCAGGTGCCCGTGGAGAGGGCAGTGGAAGAAGGCAGGGATGGTCAG GATAGATCTTAGGAGACAAGAGTGTGCTGTGGAAGATGCCCACTCTCCAG |
| mm4_exon_Nrip1_chr16_76372990 |
TCCCTCCTTCCCAGGTTAGCCGTGCCCTGCGGCCGCGCGAGGCCACGGGCGCTGCGCTCGGGCGGGCTGGGGCCGGCTCG TCCTCCGCCTGCGCCGCGGTCGCCGTCGCCTCCCGAGTTCGGGGCTCTGCTGCAATGTCT |
| mm4_intergenic_Bcl11b|Setd3_chr12_108006177 |
AGCTTAGGGGTCCAGGAGTCAGCTGCCTGCTAGAGCACGGGGGGACCGGGGACAGGGCAGCGGGAAGCAGCGGCCGCAGT CCGGACTGACCCCAG |
| mm4_exon_Wfs1_chr5_36988907 |
ACCCTGAAGAACTTACCGGCCGCAGTGCTGGGCCTCCCAGCCCGCCGCGAAGTTGCTCCGAAACCGCGGAGATTGCAGCG GCCAGCTGAGCAGGCGGAGCGCGCGGCCCTACATTCAG |
| mm4_exon_Mtmr2_chr9_13749279 | GCTCCTCCCTCTGCATCTCGCGCCGGCGGCCGCGGAGGGCGCAGCGGCCTCCAGTTTCTCGCGCCATGGAGAAG |