CRISPOR

DNA break repair outcome predictions

Guide: TTCTTCATAGAAATCCGATT CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGGGTGCAGCTCACCTCGATTTCTTCATAGAAATCCGATTCGGTGTCTGAGCTACTAGGTTGCCCTTGGCCAGAGAGGCT`	(Wild-type sequence)
311	`TCACCTCGATTTCTTCATAGAAATCCG-----GTGTCTGAGCTACTAGGTTGCCCTTGGC`	`5 bp deleted → frameshift`
164	`TCACCTCGATTTCTTCATAGAAATCCGA------------GCTACTAGGTTGCCCTTGGC`	`12 bp deleted → no frameshift`
164	`TCACCTCGATTTCTTCATAGAAATC------------TGAGCTACTAGGTTGCCCTTGGC`	`12 bp deleted → no frameshift`
120	`TCACCTCGATTTCT------------------------GAGCTACTAGGTTGCCCTTGGC`	`24 bp deleted → no frameshift`
120	`TCACCTCG------------------------GTGTCTGAGCTACTAGGTTGCCCTTGGC`	`24 bp deleted → no frameshift`
116	`TCACCTCGATTTCTTCATAGA-------------------GCTACTAGGTTGCCCTTGGC`	`19 bp deleted → frameshift`
105	`TCACCTCGATTTCTTCATAG---------------------CTACTAGGTTGCCCTTGGC`	`21 bp deleted → no frameshift`
105	`TCACCTCGATTTCTTC---------------------TGAGCTACTAGGTTGCCCTTGGC`	`21 bp deleted → no frameshift`
98	`TCACCTCGATTTCTTCATAGAAATCC----------------------------CTTGGC`	`28 bp deleted → frameshift`
98	`TCACCTCGATTTCTTCATAG----------------------------GTTGCCCTTGGC`	`28 bp deleted → frameshift`
94	`TCACCTCGATTTCTTCATAGAAATCC-----------------------------TTGGC`	`29 bp deleted → frameshift`
70	`TCACCTCGATTTCT-----------------------------ACTAGGTTGCCCTTGGC`	`29 bp deleted → frameshift`
70	`TCACCTCGATTTCTTCATAGAAATCCGATT---------------------GCCCTTGGC`	`21 bp deleted → no frameshift`
66	`TCACCTC------------------------------TGAGCTACTAGGTTGCCCTTGGC`	`30 bp deleted → no frameshift`
63	`TCACCTCGA-------------------------------GCTACTAGGTTGCCCTTGGC`	`31 bp deleted → frameshift`
60	`TCACCTCGATTTCT--------------------------------AGGTTGCCCTTGGC`	`32 bp deleted → frameshift`
60	`TCACCT--------------------------------GAGCTACTAGGTTGCCCTTGGC`	`32 bp deleted → frameshift`
57	`TCACCTCGATTTCTTCATA-------------------------CTAGGTTGCCCTTGGC`	`25 bp deleted → frameshift`
52	`TC-----------------------------------TGAGCTACTAGGTTGCCCTTGGC`	`35 bp deleted → frameshift`
51	`TCACCTCGATTTCTTCATAGAAATCCGATT---------------------------GGC`	`27 bp deleted → no frameshift`
48	`TCACCTCGATTTCTT------------------------------------------GGC`	`42 bp deleted → no frameshift`
47	`TCACCT-------------------------------------ACTAGGTTGCCCTTGGC`	`37 bp deleted → frameshift`
41	`TCACCT--------------------------------------------------TGGC`	`50 bp deleted → frameshift`
40	`TCACCT----------------------------------------AGGTTGCCCTTGGC`	`40 bp deleted → frameshift`
38	`TCAC-----------------------------------------TAGGTTGCCCTTGGC`	`41 bp deleted → frameshift`
34	`TCACC-------------------------------------------------CTTGGC`	`49 bp deleted → frameshift`
33	`TCACCTCGATTTCTT------------------------------------GCCCTTGGC`	`36 bp deleted → no frameshift`
28	`TCACCTCGATTT---------------------------------------GCCCTTGGC`	`39 bp deleted → no frameshift`
27	`TCACCTCGATT----------------------------------------GCCCTTGGC`	`40 bp deleted → frameshift`
21	`TCACCTCGATTT---------------------------------------------GGC`	`45 bp deleted → no frameshift`
20	`TCACCTCGATT----------------------------------------------GGC`	`46 bp deleted → frameshift`

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