CRISPOR

DNA break repair outcome predictions

Guide: TAAGGCGACAAGGCAGGCGC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TCTTGGCTGAGAAGAGGAGCTAAGGCGACAAGGCAGGCGCCGGGTAGCCCCAGCGCCGTGTCGACGGCGACCGCGGAGAT`	(Wild-type sequence)
359	`GAAGAGGAGCTAAGGCGACAAGGCAGGCGC----------------CGTGTCGACGGCGA`	`16 bp deleted → frameshift`
344	`GAAGAGGAGCTAAGGCGACAAGGCAGGCG---GGTAGCCCCAGCGCCGTGTCGACGGCGA`	`3 bp deleted → no frameshift`
296	`GAAGAGGAGCTAAGGCGACAAGGCAGG------GTAGCCCCAGCGCCGTGTCGACGGCGA`	`6 bp deleted → no frameshift`
282	`GAAGAGGAGCTAAGGCGACAAGGCAGG-------TAGCCCCAGCGCCGTGTCGACGGCGA`	`7 bp deleted → frameshift`
268	`GAAGAGGAGCTAAGGCGACAAGGCAGGCGC--------CCCAGCGCCGTGTCGACGGCGA`	`8 bp deleted → frameshift`
242	`GAAGAGGAGCTAAGGCGACAAGGCAGGC----------CCCAGCGCCGTGTCGACGGCGA`	`10 bp deleted → frameshift`
242	`GAAGAGGAGCTAAGGCGACAAGG----------GTAGCCCCAGCGCCGTGTCGACGGCGA`	`10 bp deleted → frameshift`
230	`GAAGAGGAGCTAAGGCGACAAGG-----------TAGCCCCAGCGCCGTGTCGACGGCGA`	`11 bp deleted → frameshift`
213	`GAAGAGGAGCTAAGGCGACAAGGCAG-----------------CGCCGTGTCGACGGCGA`	`17 bp deleted → frameshift`
198	`GAAGAGGAGCTAAGGCGACAAGGCAGGCGC--------------GCCGTGTCGACGGCGA`	`14 bp deleted → frameshift`
198	`GAAGAGGAGCTAAGGCGACAAGGC--------------CCCAGCGCCGTGTCGACGGCGA`	`14 bp deleted → frameshift`
188	`GAAGAGGAGCTAAGGCG---------------GGTAGCCCCAGCGCCGTGTCGACGGCGA`	`15 bp deleted → no frameshift`
178	`GAAGAGGAGCTAAGGCGACAAGGCAGGCG------------------------------A`	`30 bp deleted → no frameshift`
173	`GAAGAGGAGCTAAGGCGACAAGGCAG-----------CCCCAGCGCCGTGTCGACGGCGA`	`11 bp deleted → frameshift`
162	`GAAGAGGAGCTAAGGCGACAAGGCAGGC------------------CGTGTCGACGGCGA`	`18 bp deleted → no frameshift`
162	`GAAGAGGAGCTAAGG------------------GTAGCCCCAGCGCCGTGTCGACGGCGA`	`18 bp deleted → no frameshift`
154	`GAAGAGGAGCTAAGGCGACAAGGCAGGCG-------------------TGTCGACGGCGA`	`19 bp deleted → frameshift`
154	`GAAGAGGAGCTAAGG-------------------TAGCCCCAGCGCCGTGTCGACGGCGA`	`19 bp deleted → frameshift`
148	`GAAGAGGAGCTAAGGCG----------------------------CCGTGTCGACGGCGA`	`28 bp deleted → frameshift`
147	`GAAGAGGAGCTAAGGCGACAAGGC--------------------GCCGTGTCGACGGCGA`	`20 bp deleted → frameshift`
141	`GAAGAGGAGCTAAGGCGACAAG---------------CCCCAGCGCCGTGTCGACGGCGA`	`15 bp deleted → no frameshift`
133	`GAAGAGGAGCTAAGGCGACAAGGC----------------------CGTGTCGACGGCGA`	`22 bp deleted → frameshift`
133	`GAAGAGGAGCTAAGGC----------------------CCCAGCGCCGTGTCGACGGCGA`	`22 bp deleted → frameshift`
123	`GAAGAGGAGC----------------------------CCCAGCGCCGTGTCGACGGCGA`	`28 bp deleted → frameshift`
120	`GAAGAGGAGCTAAGGCGACAAGGCAGGCG------------------------ACGGCGA`	`24 bp deleted → no frameshift`
115	`GAAGAGGAGCTAAGGCGAC------------------------------------GGCGA`	`36 bp deleted → no frameshift`
109	`GAAGAGGAGCTAAGGCGACAAGGC----------------------------------GA`	`34 bp deleted → frameshift`
109	`GAAGAGGAGCTAAGGCGA------------------------------------------`	`42 bp deleted → no frameshift`
109	`GAAGAGG--------------------------GTAGCCCCAGCGCCGTGTCGACGGCGA`	`26 bp deleted → frameshift`
105	`GAAGAGGAGCTAAGGCGACAAG---------------------CGCCGTGTCGACGGCGA`	`21 bp deleted → no frameshift`
103	`GAAGAGGAGCTAAGGCGACAAGGCAGGCG---------------------------GCGA`	`27 bp deleted → no frameshift`
103	`GAAGAGG---------------------------TAGCCCCAGCGCCGTGTCGACGGCGA`	`27 bp deleted → no frameshift`
99	`GAAGAGGAGCTAAGGCGACA----------------------GCGCCGTGTCGACGGCGA`	`22 bp deleted → frameshift`
98	`GAAGAGGAGCTAAGGCGACAAGGCAGGCGC----------------------------GA`	`28 bp deleted → frameshift`
95	`GAAGAGGAGCTAAG-----------------------CCCCAGCGCCGTGTCGACGGCGA`	`23 bp deleted → frameshift`
91	`GAAGAGGAGC----------------------------------GCCGTGTCGACGGCGA`	`34 bp deleted → frameshift`
89	`GAAGAGGAGCTAAGGC------------------------------CGTGTCGACGGCGA`	`30 bp deleted → no frameshift`
84	`GAAGAGGAGCTAAGGCG-------------------------------TGTCGACGGCGA`	`31 bp deleted → frameshift`
70	`GAAGAGGAGCTAAG-----------------------------CGCCGTGTCGACGGCGA`	`29 bp deleted → frameshift`
66	`GAAGAGGAGC------------------------------------CGTGTCGACGGCGA`	`36 bp deleted → no frameshift`
63	`GAAGAG-------------------------------CCCCAGCGCCGTGTCGACGGCGA`	`31 bp deleted → frameshift`
60	`GAAGAGGAGCTA------------------------GCCCCAGCGCCGTGTCGACGGCGA`	`24 bp deleted → no frameshift`
57	`GAAG---------------------------------CCCCAGCGCCGTGTCGACGGCGA`	`33 bp deleted → no frameshift`
56	`GAAGAGGAGCTAAGGCG---------------------------------------GCGA`	`39 bp deleted → no frameshift`
47	`GAAGAG-------------------------------------CGCCGTGTCGACGGCGA`	`37 bp deleted → frameshift`
42	`GAAG---------------------------------------CGCCGTGTCGACGGCGA`	`39 bp deleted → no frameshift`
36	`GAAGAGGAGC------------------------------------------------GA`	`48 bp deleted → no frameshift`
32	`GAAGAGG--------------------------------------------------CGA`	`50 bp deleted → frameshift`
30	`GAAGAGGA----------------------------------------------CGGCGA`	`46 bp deleted → frameshift`
25	`GAAGA-------------------------------------------------CGGCGA`	`49 bp deleted → frameshift`
22	`GAAGAGGA----------------------------------------------------`	`52 bp deleted → frameshift`
22	`GA----------------------------------------------------CGGCGA`	`52 bp deleted → frameshift`
19	`GAAGA-------------------------------------------------------`	`55 bp deleted → frameshift`
16	`GA----------------------------------------------------------`	`58 bp deleted → frameshift`

Version 5.01 - Documentation - Contact us - Downloads/local installation - Citation - License