CRISPOR

DNA break repair outcome predictions

Guide: GCTGTCCGCGGAGGGGACTG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGTTGCCCTTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCATGGCCGCTCCAGCAGACCGTGG`	(Wild-type sequence)
536	`GGCCAGAGAGGCTGTCCGCGGAGGGG--------TCCCGGGCCAAGCATGGCCGCTCCAG`	`8 bp deleted → frameshift`
423	`GGCCAGAGAGGCTGTCCGCGGAGGGG-------GTCCCGGGCCAAGCATGGCCGCTCCAG`	`7 bp deleted → frameshift`
382	`GGCCAGAGAGGCTGTCCGCGGAGGG---------TCCCGGGCCAAGCATGGCCGCTCCAG`	`9 bp deleted → no frameshift`
296	`GGCCAGAGAGGCTGTCCGCGGAGGGG------GGTCCCGGGCCAAGCATGGCCGCTCCAG`	`6 bp deleted → no frameshift`
283	`GGCCAGAGAGGCTGTCCGCGGAGGGG---------------CCAAGCATGGCCGCTCCAG`	`15 bp deleted → no frameshift`
269	`GGCCAGAGAGGCTGTCCGCGGAGGG----------------CCAAGCATGGCCGCTCCAG`	`16 bp deleted → frameshift`
257	`GGCCAGAGAGGCTGTCC--------------------CGGGCCAAGCATGGCCGCTCCAG`	`20 bp deleted → frameshift`
242	`GGCCAGAGAGGCTGTCCGCGGAGG----------TCCCGGGCCAAGCATGGCCGCTCCAG`	`10 bp deleted → frameshift`
232	`GGCCAGAGAGGCTGTCCGCGG-------------------GCCAAGCATGGCCGCTCCAG`	`19 bp deleted → frameshift`
230	`GGCCAGAGAGGCTGTCCGCGG-----------GGTCCCGGGCCAAGCATGGCCGCTCCAG`	`11 bp deleted → frameshift`
219	`GGCCAGAGAGGCTGTCCGCGG------------GTCCCGGGCCAAGCATGGCCGCTCCAG`	`12 bp deleted → no frameshift`
210	`GGCCAGAGAGGCTGTCCG---------------------GGCCAAGCATGGCCGCTCCAG`	`21 bp deleted → no frameshift`
208	`GGCCAGAGAGGCTGTCCGCGG-------------TCCCGGGCCAAGCATGGCCGCTCCAG`	`13 bp deleted → frameshift`
198	`GGCCAGAGAGGCTGTCCGCGGAGGGG--------------GCCAAGCATGGCCGCTCCAG`	`14 bp deleted → frameshift`
170	`GGCCAGAGAGGCTGTCCGCGGAGG-----------------CCAAGCATGGCCGCTCCAG`	`17 bp deleted → frameshift`
147	`GGCCAGAGAGGCTGTCCGCGG--------------------CCAAGCATGGCCGCTCCAG`	`20 bp deleted → frameshift`
140	`GGCCAGAGAGG---------------------GGTCCCGGGCCAAGCATGGCCGCTCCAG`	`21 bp deleted → no frameshift`
133	`GGCCAGAGAGGC------------------------------CAAGCATGGCCGCTCCAG`	`30 bp deleted → no frameshift`
133	`GGCCAGAGAGG----------------------GTCCCGGGCCAAGCATGGCCGCTCCAG`	`22 bp deleted → frameshift`
132	`GGCCAGAGAGGCTGTCCGC------------------------------------TCCAG`	`36 bp deleted → no frameshift`
127	`GGCCA---------------------------------------AGCATGGCCGCTCCAG`	`39 bp deleted → no frameshift`
126	`GGCCAGAGAGGCTGTCCGC-----------------------CAAGCATGGCCGCTCCAG`	`23 bp deleted → frameshift`
126	`GGCCAGAGAGG-----------------------TCCCGGGCCAAGCATGGCCGCTCCAG`	`23 bp deleted → frameshift`
114	`GGCCAGAGAGGCTGTCCGCGGAGGGG-------------------------CCGCTCCAG`	`25 bp deleted → frameshift`
110	`GGCCAGAGAGGCTGTCCGCGGAGGGGACTG--------------------GCCGCTCCAG`	`20 bp deleted → frameshift`
109	`GGCCAGAGAGGCTGTCCGCGGAGGG--------------------------CCGCTCCAG`	`26 bp deleted → frameshift`
109	`GGCCAGAGAGGCTGTCC--------------------------AAGCATGGCCGCTCCAG`	`26 bp deleted → frameshift`
103	`GGCCAGAGAGGCTGTCCGCGGAGG---------------------------CCGCTCCAG`	`27 bp deleted → no frameshift`
98	`GGCCAGAGAGGCTGTCCGC----------------------------ATGGCCGCTCCAG`	`28 bp deleted → frameshift`
95	`GGCCAGAGAGGCTGTCCGCGGAG-----------------------CATGGCCGCTCCAG`	`23 bp deleted → frameshift`
94	`GGCCAGAGAGG-----------------------------GCCAAGCATGGCCGCTCCAG`	`29 bp deleted → frameshift`
89	`GGCCAGAGAGGCTGTCCGCGG------------------------------CCGCTCCAG`	`30 bp deleted → no frameshift`
89	`GG------------------------------GGTCCCGGGCCAAGCATGGCCGCTCCAG`	`30 bp deleted → no frameshift`
84	`GG-------------------------------GTCCCGGGCCAAGCATGGCCGCTCCAG`	`31 bp deleted → frameshift`
81	`GGCCAGAGAGGC----------------------------------------CGCTCCAG`	`40 bp deleted → frameshift`
80	`GG--------------------------------TCCCGGGCCAAGCATGGCCGCTCCAG`	`32 bp deleted → frameshift`
77	`GGCCAGAGAGGCTGTCCGCGGAGGGGACT---------------------------CCAG`	`27 bp deleted → no frameshift`
76	`GGCCAGAGAGGCTGTCCGC---------------------------------CGCTCCAG`	`33 bp deleted → no frameshift`
76	`GGCC---------------------------------CGGGCCAAGCATGGCCGCTCCAG`	`33 bp deleted → no frameshift`
73	`GGCCAGAGAGGCTGTCCGCG----------------------------------CTCCAG`	`34 bp deleted → frameshift`
73	`GGCC----------------------------------GGGCCAAGCATGGCCGCTCCAG`	`34 bp deleted → frameshift`
69	`GGCCAGAGAGGC-----------------------------------ATGGCCGCTCCAG`	`35 bp deleted → frameshift`
68	`GGCC-------------------------------------------------GCTCCAG`	`49 bp deleted → frameshift`
64	`GGCCAGAGAGGCTGTCC-----------------------------------------AG`	`41 bp deleted → frameshift`
60	`GG--------------------------------------GCCAAGCATGGCCGCTCCAG`	`38 bp deleted → frameshift`
58	`GGCCAGAGAGGCT-------------------------------------------CCAG`	`43 bp deleted → frameshift`
49	`GGCCAGAGAGGCTG------------------------------------GCCGCTCCAG`	`36 bp deleted → no frameshift`
49	`GGCCAGAGAG------------------------------------CATGGCCGCTCCAG`	`36 bp deleted → no frameshift`
47	`GGCCAGAGAGGCTGTCCGCGGAG-------------------------------------`	`37 bp deleted → frameshift`
46	`GGCCAG------------------------------------------------------`	`54 bp deleted → no frameshift`
45	`GGCCAGAG--------------------------------------CATGGCCGCTCCAG`	`38 bp deleted → frameshift`
44	`GGC--------------------------------------------ATGGCCGCTCCAG`	`44 bp deleted → frameshift`
40	`GGCCAG----------------------------------------CATGGCCGCTCCAG`	`40 bp deleted → frameshift`
34	`GGCCA-------------------------------------------TGGCCGCTCCAG`	`43 bp deleted → frameshift`
29	`GGC----------------------------------------------------TCCAG`	`52 bp deleted → frameshift`
24	`GGCCAGAGAG--------------------------------------------------`	`50 bp deleted → frameshift`
22	`GGCCAGAG----------------------------------------------------`	`52 bp deleted → frameshift`

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