CRISPOR

DNA break repair outcome predictions

Guide: GAGGGCCGGCTCGCGGGCTC AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCCACGCTCCCGGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGTGGGGCACCATCTTCTCTGGCGGTTCAGGCA`	(Wild-type sequence)
444	`CGGGAAGTCGGAGGGCCGGCTCGCGGGC------AACCGTGGGGCACCATCTTCTCTGGC`	`6 bp deleted → no frameshift`
341	`CGGGAAGTCGGAGGGCCGGCTCGCGGGC-----------------ACCATCTTCTCTGGC`	`17 bp deleted → frameshift`
298	`CGGGAAGTCGGAGGGCCGGC--------------AACCGTGGGGCACCATCTTCTCTGGC`	`14 bp deleted → frameshift`
282	`CGGGAAGTCGGAGGGCCGGCTCGCGG-------CAACCGTGGGGCACCATCTTCTCTGGC`	`7 bp deleted → frameshift`
269	`CGGGAAGTCGGAGGGCCGGCTCGCGGG----------------GCACCATCTTCTCTGGC`	`16 bp deleted → frameshift`
244	`CGGGAAGTCGGAGGGC------------------AACCGTGGGGCACCATCTTCTCTGGC`	`18 bp deleted → no frameshift`
242	`CGGGAAGTCGGAGGGCCGGCTCGC----------AACCGTGGGGCACCATCTTCTCTGGC`	`10 bp deleted → frameshift`
210	`CGGGAAGTCGGAGGGCCG---------------------TGGGGCACCATCTTCTCTGGC`	`21 bp deleted → no frameshift`
198	`CGGGAAGTCGGAGGGCCGGCTCGCG--------------TGGGGCACCATCTTCTCTGGC`	`14 bp deleted → frameshift`
193	`CGGGAAGTCGGAGG-------------------CAACCGTGGGGCACCATCTTCTCTGGC`	`19 bp deleted → frameshift`
188	`CGGGAAGTCGGAGGGCCGGCTCGCGGG---------------GGCACCATCTTCTCTGGC`	`15 bp deleted → no frameshift`
188	`CGGGAAGTCGGAGGGC-----------------------------ACCATCTTCTCTGGC`	`29 bp deleted → frameshift`
179	`CGGGAAGTCGGAGGGCCGGCTCG----------------TGGGGCACCATCTTCTCTGGC`	`16 bp deleted → frameshift`
172	`CGGGAAGTCGGAGGGCCGGC-------------------------ACCATCTTCTCTGGC`	`25 bp deleted → frameshift`
162	`CGGGAAGTCGGAGGGCCGGCTCGCGG------------------CACCATCTTCTCTGGC`	`18 bp deleted → no frameshift`
148	`CGGGAAGTCGGAGGG----------------------------GCACCATCTTCTCTGGC`	`28 bp deleted → frameshift`
140	`CGGGAAGTCGGAGGGCCGGCTCGC---------------------ACCATCTTCTCTGGC`	`21 bp deleted → no frameshift`
136	`CGGGAAGTCGGAGGGCCGGCTCGCGGGCTC--------------------------TGGC`	`26 bp deleted → frameshift`
133	`CGGGAAGTCGG----------------------CAACCGTGGGGCACCATCTTCTCTGGC`	`22 bp deleted → frameshift`
126	`CGGGAAGTCGGAGGGCCGG-----------------------GGCACCATCTTCTCTGGC`	`23 bp deleted → frameshift`
121	`CGGGAAGTCGGAGGGCCGGCTCGCGGGC--------------------------------`	`32 bp deleted → frameshift`
120	`CGGGAAGTCGGAGGGCCGG------------------------GCACCATCTTCTCTGGC`	`24 bp deleted → no frameshift`
105	`CGGGAAGTCGGAGGGCCGGCTCGCGGGCTC---------------------TTCTCTGGC`	`21 bp deleted → no frameshift`
103	`CGGGAAGTCGGAGGG---------------------------GGCACCATCTTCTCTGGC`	`27 bp deleted → no frameshift`
95	`CGGGAAGTCGGAGGGCCGGCTCGCGGGCT-----------------------TCTCTGGC`	`23 bp deleted → frameshift`
94	`CGGGAAGTCG-----------------------------TGGGGCACCATCTTCTCTGGC`	`29 bp deleted → frameshift`
94	`CGGG-----------------------------CAACCGTGGGGCACCATCTTCTCTGGC`	`29 bp deleted → frameshift`
91	`CGGGAAGTCGGAGGGCCGGCTC----------------------------------TGGC`	`34 bp deleted → frameshift`
90	`CGGGAAGTCGGAGGGCCGGCTCGCGGGCTC------------------------TCTGGC`	`24 bp deleted → no frameshift`
89	`CGGGAAGTCGGAGG------------------------------CACCATCTTCTCTGGC`	`30 bp deleted → no frameshift`
89	`CGG------------------------------CAACCGTGGGGCACCATCTTCTCTGGC`	`30 bp deleted → no frameshift`
86	`CGGGAAG-------------------------GCAACCGTGGGGCACCATCTTCTCTGGC`	`25 bp deleted → frameshift`
85	`CGGG---------------------------------------GCACCATCTTCTCTGGC`	`39 bp deleted → no frameshift`
84	`CGGGAAGTCGGAGGGCC-------------------------------ATCTTCTCTGGC`	`31 bp deleted → frameshift`
84	`CGGGAAGTCGG-------------------------------GGCACCATCTTCTCTGGC`	`31 bp deleted → frameshift`
81	`CGGGAAGTCGGAGGGCCGGC----------------------------------------`	`40 bp deleted → frameshift`
81	`CGGG----------------------------------------CACCATCTTCTCTGGC`	`40 bp deleted → frameshift`
80	`CGGGAAGTCGG--------------------------------GCACCATCTTCTCTGGC`	`32 bp deleted → frameshift`
76	`CGGGAAGTCGGAGGGCCGGCTCGCGG---------------------------------C`	`33 bp deleted → no frameshift`
76	`CGGGAAGTCGG---------------------------------CACCATCTTCTCTGGC`	`33 bp deleted → no frameshift`
74	`CGGGAAGTCGGAGGGCCGGCTCGCGGGCT----------------------------GGC`	`28 bp deleted → frameshift`
70	`CGGGAAGTCGGAGGGCCGGCTC-----------------------------TTCTCTGGC`	`29 bp deleted → frameshift`
66	`CGGGAAGTCGGAGGGC--------------------------------------------`	`44 bp deleted → frameshift`
66	`CGGGAAGTCGGAGGGCCGGCTCGC------------------------------------`	`36 bp deleted → no frameshift`
63	`CGGGAAGTCGGAGGGCCGGCT-------------------------------TCTCTGGC`	`31 bp deleted → frameshift`
62	`CG-------------------------------------TGGGGCACCATCTTCTCTGGC`	`37 bp deleted → frameshift`
60	`CGGGAAGTCGGAGGGCCGGCTC--------------------------------TCTGGC`	`32 bp deleted → frameshift`
60	`CGGGAAGT--------------------------------GGGGCACCATCTTCTCTGGC`	`32 bp deleted → frameshift`
60	`CGGG--------------------------------------GGCACCATCTTCTCTGGC`	`38 bp deleted → frameshift`
51	`CGG-----------------------------------------CACCATCTTCTCTGGC`	`41 bp deleted → frameshift`
49	`CGGGAAGTCGGAGGGCCGGCT------------------------------------GGC`	`36 bp deleted → no frameshift`
44	`CGGGAA------------------------------CCGTGGGGCACCATCTTCTCTGGC`	`30 bp deleted → no frameshift`
42	`CGGGAAGTCGGAGG---------------------------------------------C`	`45 bp deleted → no frameshift`
36	`CGGGAAGTC------------------------------------------TTCTCTGGC`	`42 bp deleted → no frameshift`
36	`CGGGAAGTCGG------------------------------------------------C`	`48 bp deleted → no frameshift`
31	`CGGGAAGTC---------------------------------------------TCTGGC`	`45 bp deleted → no frameshift`
28	`CGGGAAGTC-----------------------------------------------TGGC`	`47 bp deleted → frameshift`
25	`CGGG-------------------------------------------------------C`	`55 bp deleted → frameshift`
24	`CGG--------------------------------------------------------C`	`56 bp deleted → frameshift`

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