CRISPOR

DNA break repair outcome predictions

Guide: GCAGGCAGTGCTGCAGGGCT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGGAGCCGGTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGC`	(Wild-type sequence)
623	`CGATGGACTAGCAGGCAGTGCTGCAGGGC-----GCCGAGCCACGGTCTGCTGGAGCGGC`	`5 bp deleted → frameshift`
327	`CGATGGACTAGCAGGCAGTGCTGCAGGG----GCGCCGAGCCACGGTCTGCTGGAGCGGC`	`4 bp deleted → frameshift`
296	`CGATGGACTAGCAGGCAGTGCTGCAGG------CGCCGAGCCACGGTCTGCTGGAGCGGC`	`6 bp deleted → no frameshift`
282	`CGATGGACTAGCAGGCAGTGCTGCAGGGC-------CGAGCCACGGTCTGCTGGAGCGGC`	`7 bp deleted → frameshift`
244	`CGATGGACTAGCAGGC------------------GCCGAGCCACGGTCTGCTGGAGCGGC`	`18 bp deleted → no frameshift`
242	`CGATGGACTAGCAGGCAGTGCTGC----------GCCGAGCCACGGTCTGCTGGAGCGGC`	`10 bp deleted → frameshift`
219	`CGATGGACTAGCAGGCAGTGCTGCAGGGC------------CACGGTCTGCTGGAGCGGC`	`12 bp deleted → no frameshift`
219	`CGATGGACTAGCAGGCAGTGCTGC------------CGAGCCACGGTCTGCTGGAGCGGC`	`12 bp deleted → no frameshift`
208	`CGATGGACTAGCAGGCAGTGC-------------GCCGAGCCACGGTCTGCTGGAGCGGC`	`13 bp deleted → frameshift`
188	`CGATGGACTAGCAGGCAGTGC---------------CGAGCCACGGTCTGCTGGAGCGGC`	`15 bp deleted → no frameshift`
181	`CGATGGACTAGCAGGCAGTGCTGC---------------------------TGGAGCGGC`	`27 bp deleted → no frameshift`
178	`CGATGGACTAGCAGGCAGTGCTG------------------------------GAGCGGC`	`30 bp deleted → no frameshift`
170	`CGATGGACTAGCAGGCAGTGCTGC-----------------CACGGTCTGCTGGAGCGGC`	`17 bp deleted → frameshift`
170	`CGATGGACTAGCAGG-----------------GCGCCGAGCCACGGTCTGCTGGAGCGGC`	`17 bp deleted → frameshift`
166	`CGATGGACTAGCAGGCAGTGCTGCAGGGCT----------------------GGAGCGGC`	`22 bp deleted → frameshift`
162	`CGATGGACTAGCAGGCAGTGCTGCAGGG------------------TCTGCTGGAGCGGC`	`18 bp deleted → no frameshift`
154	`CGATGGACTAGCAGGCAGTGCTGCAGG-------------------TCTGCTGGAGCGGC`	`19 bp deleted → frameshift`
149	`CGATGGACTAGCAGGCAGTGCTGCAG--------------CCACGGTCTGCTGGAGCGGC`	`14 bp deleted → frameshift`
147	`CGATGGACTAGCAGGCAGTGC--------------------CACGGTCTGCTGGAGCGGC`	`20 bp deleted → frameshift`
147	`CGATGGACTAGCAGGC--------------------CGAGCCACGGTCTGCTGGAGCGGC`	`20 bp deleted → frameshift`
133	`CGATGGACTAGC----------------------GCCGAGCCACGGTCTGCTGGAGCGGC`	`22 bp deleted → frameshift`
127	`CGATGGACTAGCAGGCAGTGCTGCAGGGC-------------------------------`	`31 bp deleted → frameshift`
122	`CGATGGACTAGCAGGCAGTGCTGCA------------------CGGTCTGCTGGAGCGGC`	`18 bp deleted → no frameshift`
120	`CGATGGACTAGC------------------------CGAGCCACGGTCTGCTGGAGCGGC`	`24 bp deleted → no frameshift`
117	`CGATGGACTAGC-----------------------------CACGGTCTGCTGGAGCGGC`	`29 bp deleted → frameshift`
116	`CGATGGACTAGCAGGCAGTGCTGCAGGGCT-------------------GCTGGAGCGGC`	`19 bp deleted → frameshift`
114	`CGATGGACTAGCAGGC-------------------------CACGGTCTGCTGGAGCGGC`	`25 bp deleted → frameshift`
109	`CGATGGACTAGCAGGCAGTGCTGCAGGG--------------------------AGCGGC`	`26 bp deleted → frameshift`
109	`CGATGG--------------------------GCGCCGAGCCACGGTCTGCTGGAGCGGC`	`26 bp deleted → frameshift`
103	`CGATGGACTAGCAGGCAGTGCTGCAGG---------------------------AGCGGC`	`27 bp deleted → no frameshift`
103	`CGATGG---------------------------CGCCGAGCCACGGTCTGCTGGAGCGGC`	`27 bp deleted → no frameshift`
99	`CGATGGACTAGCAGGCAG----------------------CCACGGTCTGCTGGAGCGGC`	`22 bp deleted → frameshift`
98	`CGATGGACTAGCAGGCAGTGCTGCAGGGC----------------------------GGC`	`28 bp deleted → frameshift`
84	`CGATGGACTAGCAGG-------------------------------TCTGCTGGAGCGGC`	`31 bp deleted → frameshift`
82	`CGA------------------------------------GCCACGGTCTGCTGGAGCGGC`	`36 bp deleted → no frameshift`
81	`CGATGGACTAGCAGGCA--------------------------CGGTCTGCTGGAGCGGC`	`26 bp deleted → frameshift`
81	`CGATGGACTAGCAG--------------------------CCACGGTCTGCTGGAGCGGC`	`26 bp deleted → frameshift`
80	`CGATGGACTAGCAGGCAGTGCTGCAGG--------------------------------C`	`32 bp deleted → frameshift`
76	`CGATGGACTAGCAGGCAGTGCTGC---------------------------------GGC`	`33 bp deleted → no frameshift`
76	`CG---------------------------------CCGAGCCACGGTCTGCTGGAGCGGC`	`33 bp deleted → no frameshift`
74	`CGATGGACTAGCAGGCAGT----------------------------CTGCTGGAGCGGC`	`28 bp deleted → frameshift`
69	`CGATGGACTAGCAGGC-----------------------------------TGGAGCGGC`	`35 bp deleted → frameshift`
66	`CGATGGACTAGCAGGCAGTGCTGCAG------------------------------CGGC`	`30 bp deleted → no frameshift`
66	`CGATGGACTAGCA------------------------------CGGTCTGCTGGAGCGGC`	`30 bp deleted → no frameshift`
66	`CGATGGACTAGCAGGC--------------------------------------------`	`44 bp deleted → frameshift`
66	`CGATGGACTAGCAGGCAGTGCTGC------------------------------------`	`36 bp deleted → no frameshift`
66	`CGATGGACTAGCAGGCAGTGC------------------------------------GGC`	`36 bp deleted → no frameshift`
60	`CGATGGA--------------------------------GCCACGGTCTGCTGGAGCGGC`	`32 bp deleted → frameshift`
57	`CGATGGACTAGCAGGCAGTG---------------------------------GAGCGGC`	`33 bp deleted → no frameshift`
56	`CGATGGACTAGCAGGCAGTGC---------------------------------------`	`39 bp deleted → no frameshift`
56	`CGATGGACTAGCAGG---------------------------------------AGCGGC`	`39 bp deleted → no frameshift`
56	`CGATGGACTAGC---------------------------------------TGGAGCGGC`	`39 bp deleted → no frameshift`
54	`CGATGGA------------------------------------------------GCGGC`	`48 bp deleted → no frameshift`
54	`CGATGG----------------------------------------TCTGCTGGAGCGGC`	`40 bp deleted → frameshift`
52	`CGATGGACTAGC---------------------------------------------GGC`	`45 bp deleted → no frameshift`
51	`CGATGGACTAGCAGGC-----------------------------------------GGC`	`41 bp deleted → frameshift`
49	`CGATGGAC------------------------------------GGTCTGCTGGAGCGGC`	`36 bp deleted → no frameshift`
46	`CG-------------------------------------------GTCTGCTGGAGCGGC`	`43 bp deleted → frameshift`
45	`CGATGGACTAGCAGGCAG--------------------------------------CGGC`	`38 bp deleted → frameshift`
40	`CGATGGACT----------------------------------------GCTGGAGCGGC`	`40 bp deleted → frameshift`
36	`CGATGGACTAGCAG------------------------------------------CGGC`	`42 bp deleted → no frameshift`
36	`CGATGGACTAGC------------------------------------------------`	`48 bp deleted → no frameshift`
34	`CGATGGACT-------------------------------------------GGAGCGGC`	`43 bp deleted → frameshift`
31	`CGATG---------------------------------------------CTGGAGCGGC`	`45 bp deleted → no frameshift`
28	`CGATGG-----------------------------------------------------C`	`53 bp deleted → frameshift`
24	`CG--------------------------------------------------------GC`	`56 bp deleted → frameshift`
22	`CGA----------------------------------------------------GCGGC`	`52 bp deleted → frameshift`

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