CRISPOR

DNA break repair outcome predictions

Guide: CCGACTTCCCGGGAGCGTGG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCA`	(Wild-type sequence)
469	`AGCCGGCCCTCCGACTTCCCGGGAG-------------GCCAGGAGGAGGAGCCGGTCGA`	`13 bp deleted → frameshift`
461	`AGCCGGCCCTCCGACTTCCCGGG-----------GGAGGCCAGGAGGAGGAGCCGGTCGA`	`11 bp deleted → frameshift`
344	`AGCCGGCCCTCCGACTTCCCGGGAGCGTGG---GGGAGGCCAGGAGGAGGAGCCGGTCGA`	`3 bp deleted → no frameshift`
329	`AGCCGGCCCTCCGACTTCCCGGG------------GAGGCCAGGAGGAGGAGCCGGTCGA`	`12 bp deleted → no frameshift`
327	`AGCCGGCCCTCCGACTTCCCGGGAGCGTGG----GGAGGCCAGGAGGAGGAGCCGGTCGA`	`4 bp deleted → frameshift`
311	`AGCCGGCCCTCCGACTTCCCGGGAGCGTGG-----GAGGCCAGGAGGAGGAGCCGGTCGA`	`5 bp deleted → frameshift`
311	`AGCCGGCCCTCCGACTTCCCGGGAGCG-----GGGGAGGCCAGGAGGAGGAGCCGGTCGA`	`5 bp deleted → frameshift`
296	`AGCCGGCCCTCCGACTTCCCGGGAGCGTGG------AGGCCAGGAGGAGGAGCCGGTCGA`	`6 bp deleted → no frameshift`
255	`AGCCGGCCCTCCGACTTCCCGGGAGCGTGG---------CCAGGAGGAGGAGCCGGTCGA`	`9 bp deleted → no frameshift`
245	`AGCCGGCCCTCCGACTTCCCGGGAG---------------------GAGGAGCCGGTCGA`	`21 bp deleted → no frameshift`
242	`AGCCGGCCCTCCGACTTCCCGGG----------GGGAGGCCAGGAGGAGGAGCCGGTCGA`	`10 bp deleted → frameshift`
233	`AGCCGGCCCTCCGACTTCCCGGGAGC---------------------------CGGTCGA`	`27 bp deleted → no frameshift`
210	`AGCCGGCCCTCCGACTTCCCGGGAG------------------------GAGCCGGTCGA`	`24 bp deleted → no frameshift`
198	`AGCCGGCCCTCCGACTTCCCGGGAGCGTGG--------------AGGAGGAGCCGGTCGA`	`14 bp deleted → frameshift`
198	`AGCCGGCCCTCCGACTTCCCGGGAGC--------------CAGGAGGAGGAGCCGGTCGA`	`14 bp deleted → frameshift`
198	`AGCCGGCCCTCCGACTTCCCGG--------------AGGCCAGGAGGAGGAGCCGGTCGA`	`14 bp deleted → frameshift`
179	`AGCCGGCCCTCCGACTTCCCGGG----------------CCAGGAGGAGGAGCCGGTCGA`	`16 bp deleted → frameshift`
170	`AGCCGGCCCTCCGACTTCCCGGGAGCGTGG-----------------AGGAGCCGGTCGA`	`17 bp deleted → frameshift`
170	`AGCCGGCCCTCCGACTTCCCGG-----------------CCAGGAGGAGGAGCCGGTCGA`	`17 bp deleted → frameshift`
155	`AGCCGG---------------------------GGGAGGCCAGGAGGAGGAGCCGGTCGA`	`27 bp deleted → no frameshift`
154	`AGCCGGCCCTCCG-------------------GGGGAGGCCAGGAGGAGGAGCCGGTCGA`	`19 bp deleted → frameshift`
153	`AGCCGGCC---------------------------------AGGAGGAGGAGCCGGTCGA`	`33 bp deleted → no frameshift`
147	`AGCCGGCCCTCCGACTTCCCGGGAGCGTGG--------------------AGCCGGTCGA`	`20 bp deleted → frameshift`
146	`AGCCGGCCCTCCGACTTCCCGG----------------------------------TCGA`	`34 bp deleted → frameshift`
140	`AGCCGGCCCTCCGACTTCCC---------------------AGGAGGAGGAGCCGGTCGA`	`21 bp deleted → no frameshift`
133	`AGCCGGCCCTCCGACTTCCCGG----------------------AGGAGGAGCCGGTCGA`	`22 bp deleted → frameshift`
133	`AGCCGGCCCTCCGACTTCC----------------------AGGAGGAGGAGCCGGTCGA`	`22 bp deleted → frameshift`
122	`AGCCGGCCCTCCGACTTCCCGGGAG------------------GAGGAGGAGCCGGTCGA`	`18 bp deleted → no frameshift`
114	`AGCCGGCCCTCCGACTTCCCGG-------------------------AGGAGCCGGTCGA`	`25 bp deleted → frameshift`
109	`AGCCGGCCCTCCGACTTCCCGGGAGCGTGG--------------------------TCGA`	`26 bp deleted → frameshift`
98	`AGCCGGCCCTCCGACTTCCCGGGAGCG----------------------------GTCGA`	`28 bp deleted → frameshift`
98	`AGCCGGCCCTCCGACTTCCCGG----------------------------AGCCGGTCGA`	`28 bp deleted → frameshift`
98	`AGCCGG----------------------------GGAGGCCAGGAGGAGGAGCCGGTCGA`	`28 bp deleted → frameshift`
95	`AGCCGGCCCTCCGA-----------------------GGCCAGGAGGAGGAGCCGGTCGA`	`23 bp deleted → frameshift`
94	`AGCC-------------------------------------AGGAGGAGGAGCCGGTCGA`	`37 bp deleted → frameshift`
94	`AGCCGGCCCTCC-----------------------------AGGAGGAGGAGCCGGTCGA`	`29 bp deleted → frameshift`
94	`AGCCGG-----------------------------GAGGCCAGGAGGAGGAGCCGGTCGA`	`29 bp deleted → frameshift`
90	`AGCCGG--------------------------------------------------TCGA`	`50 bp deleted → frameshift`
89	`AGCCGG------------------------------AGGCCAGGAGGAGGAGCCGGTCGA`	`30 bp deleted → no frameshift`
80	`AGCCGGCCCTCCGACTTCCCGGGAGCG--------------------------------A`	`32 bp deleted → frameshift`
80	`AGCCGGCCC--------------------------------AGGAGGAGGAGCCGGTCGA`	`32 bp deleted → frameshift`
76	`AGCCGGCCCTCCGACTTCCCGGG---------------------------------TCGA`	`33 bp deleted → no frameshift`
73	`AGCCGGCCCTCCG------------------------------------------GTCGA`	`42 bp deleted → no frameshift`
70	`AGCCGGCCCTCCGACTTCCCGGGAGCGT-----------------------------CGA`	`29 bp deleted → frameshift`
69	`AGCCGGCCCTCCGACTTCC-----------------------------------GGTCGA`	`35 bp deleted → frameshift`
63	`AGCCGGCCCTCCGA-------------------------------GGAGGAGCCGGTCGA`	`31 bp deleted → frameshift`
60	`AGCCGGCCCTCCGACTTCCCG--------------------------------------A`	`38 bp deleted → frameshift`
60	`AGCCGGCC----------------------------------------------GGTCGA`	`46 bp deleted → frameshift`
60	`AGCCGG--------------------------------------AGGAGGAGCCGGTCGA`	`38 bp deleted → frameshift`
54	`AGCCGGCCCTCCGA----------------------------------GGAGCCGGTCGA`	`34 bp deleted → frameshift`
51	`AGCCGG-----------------------------------------AGGAGCCGGTCGA`	`41 bp deleted → frameshift`
50	`AGCCGGCCCTCCGA----------------------------------------------`	`46 bp deleted → frameshift`
49	`AGCCGGCCCTCCGACTTCCCGGGA------------------------------------`	`36 bp deleted → no frameshift`
49	`AG------------------------------------GCCAGGAGGAGGAGCCGGTCGA`	`36 bp deleted → no frameshift`
47	`AGCCGGCCCTCCGA-------------------------------------GCCGGTCGA`	`37 bp deleted → frameshift`
44	`AGCCGG--------------------------------------------AGCCGGTCGA`	`44 bp deleted → frameshift`
42	`AGCCGGCCC---------------------------------------------GGTCGA`	`45 bp deleted → no frameshift`
40	`AGCCGGCCCTCCGACTTC----------------------------------------GA`	`40 bp deleted → frameshift`
38	`AG-----------------------------------------GAGGAGGAGCCGGTCGA`	`41 bp deleted → frameshift`
33	`AG--------------------------------------------GAGGAGCCGGTCGA`	`44 bp deleted → frameshift`
28	`AGCCGGCCCTC-----------------------------------------------GA`	`47 bp deleted → frameshift`
28	`AG-----------------------------------------------GAGCCGGTCGA`	`47 bp deleted → frameshift`
26	`AGCCG------------------------------------------------------A`	`54 bp deleted → no frameshift`

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