CRISPOR (citation) is a program that helps design, evaluate and clone guide sequences for the CRISPR/Cas9 system. CRISPOR Manual
April 2021: MAD7, Thermocas9, SpRY, exons Full list of changes
Step 1
Planning a lentiviral gene knockout screen? Use CRISPOR Batch
Sequence name (optional):
Enter a single genomic sequence, < 2300 bp, typically an exon
Clear Box - Reset to default
Text case is preserved, e.g. you can mark ATGs with lowercase.
Instead of a sequence, you can paste a chromosome range, e.g. chr1:11,130,540-11,130,751
Step 2
Select a genome

Note: pre-calculated exonic guides for this species are on the UCSC Genome Browser.
We have 721 genomes, but not yours? Search NCBI assembly and send a GCF_/GCA_ ID to CRISPOR support.
Step 3
Select a Protospacer Adjacent Motif (PAM)

See notes on enzymes in the manual.
Version 4.99 - Documentation  - Contact us - Downloads/local installation - Citation - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.