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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| mm0_exon_Pop5_chr5_115238090_F | TCGTCGGCAGCGTCTGCGGTTCAAGCACAGGTAA | 60.1 | 1.00 |
| mm0_exon_Pop5_chr5_115238090_R | GTCTCGTGGGCTCGGCTCGCACAGAAGGTACCTGC | 60.7 | 1.00 |
| mm4_intron_Sec24b_chr3_129993189_F | TCGTCGGCAGCGTCTGTGCATTGAGAGCCCTGAG | 60.0 | 0.53 |
| mm4_intron_Sec24b_chr3_129993189_R | GTCTCGTGGGCTCGGGGCTCCCTTTAGAGAACGGG | 59.8 | 0.53 |
| mm4_intron_Fam65a_chr8_105605471_F | TCGTCGGCAGCGTCGACTTGCGACCGGGTCCC | 62.7 | 0.53 |
| mm4_intron_Fam65a_chr8_105605471_R | GTCTCGTGGGCTCGGCGAAGCTGCGTTCTCCGT | 60.4 | 0.53 |
| mm4_intron_Dgkd_chr1_87853608_F | TCGTCGGCAGCGTCTCCTGCGGTCGCTGCTAC | 62.1 | 0.41 |
| mm4_intron_Dgkd_chr1_87853608_R | GTCTCGTGGGCTCGGGGGCCTCGGTCTCCTCAG | 61.1 | 0.41 |
| mm4_exon_Cul7_chr17_46653094_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_exon_Cul7_chr17_46653094_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm3_exon_Dclk2_chr3_86920092_F | TCGTCGGCAGCGTCCTTGGGGCCAGAGATGCTG | 60.4 | 0.33 |
| mm3_exon_Dclk2_chr3_86920092_R | GTCTCGTGGGCTCGGCAGCACAAGGAGCATTGAGC | 59.8 | 0.33 |
| mm4_intron_Tardbp_chr4_148626599_F | TCGTCGGCAGCGTCCTTAGGCCTTAACGACGCGG | 61.1 | 0.33 |
| mm4_intron_Tardbp_chr4_148626599_R | GTCTCGTGGGCTCGGTCGAGTGTTGCTTCGGAGAG | 59.7 | 0.33 |
| mm4_exon_Sms_chrX_157492095_F | TCGTCGGCAGCGTCCCAGGAGTTAGGAGGTGGGA | 59.9 | 0.33 |
| mm4_exon_Sms_chrX_157492095_R | GTCTCGTGGGCTCGGCGTCCCTGCTTCCTCCTCTA | 60.3 | 0.33 |
| mm3_intergenic_Klf10|Gm26863_chr15_38316306_F | TCGTCGGCAGCGTCACCCAACACTCCTACCCAGA | 59.8 | 0.29 |
| mm3_intergenic_Klf10|Gm26863_chr15_38316306_R | GTCTCGTGGGCTCGGCACCTTCGCCTTCAGTCACT | 59.9 | 0.29 |
| mm3_intron_Agpat5_chr8_18846810_F | TCGTCGGCAGCGTCCGGCTTTACTGCGTCTACCA | 60.1 | 0.27 |
| mm3_intron_Agpat5_chr8_18846810_R | GTCTCGTGGGCTCGGGAGTGTTCGCCCGGGTTG | 61.0 | 0.27 |
| mm4_exon_Abcb6_chr1_75180222_F | TCGTCGGCAGCGTCTCACTGCACAAAGAGTCGGG | 60.2 | 0.26 |
| mm4_exon_Abcb6_chr1_75180222_R | GTCTCGTGGGCTCGGGAGAGTGGGCAGTGTAGCAG | 60.1 | 0.26 |
| mm4_intron_Slc25a34_chr4_141622410_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Slc25a34_chr4_141622410_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Nufip2_chr11_77686651_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Nufip2_chr11_77686651_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm3_intergenic_Pde7a|Gm22282_chr3_19311575_F | TCGTCGGCAGCGTCGTCCGGACAGGGCTGCTC | 62.4 | 0.25 |
| mm3_intergenic_Pde7a|Gm22282_chr3_19311575_R | GTCTCGTGGGCTCGGCACGTAACTCTCCTGCGCA | 60.0 | 0.25 |
| mm4_intron_Ubp1_chr9_113931795_F | TCGTCGGCAGCGTCGCGCCTACAGCATGAGGTAA | 60.1 | 0.24 |
| mm4_intron_Ubp1_chr9_113931795_R | GTCTCGTGGGCTCGGAGAAGCGACGGGGTCCTC | 61.0 | 0.24 |
| mm4_intergenic_Adcy6|Cacnb3_chr15_98609529_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Adcy6|Cacnb3_chr15_98609529_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Pkn2_chr3_142881380_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Pkn2_chr3_142881380_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Sh3bp4_chr1_89070766_F | TCGTCGGCAGCGTCTTCCCTGCCCTCTGTGGT | 60.1 | 0.21 |
| mm4_intron_Sh3bp4_chr1_89070766_R | GTCTCGTGGGCTCGGCGCTCTCTTCGCTCCTTCTC | 60.2 | 0.21 |
| mm4_intron_Tbx1_chr16_18586502_F | TCGTCGGCAGCGTCCTTCATCCGCTTTTCAGCCG | 59.9 | 0.20 |
| mm4_intron_Tbx1_chr16_18586502_R | GTCTCGTGGGCTCGGATGATCGTCACCAAGGCAGG | 60.1 | 0.20 |
| mm4_intron_Kat6a_chr8_22859817_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Kat6a_chr8_22859817_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm5449|Drd1a_chr13_53639994_F | TCGTCGGCAGCGTCGTGTGGTAGGAGGCAGGAAC | 60.0 | 0.19 |
| mm4_intergenic_Gm5449|Drd1a_chr13_53639994_R | GTCTCGTGGGCTCGGACCAACCTCCTGAGCCCATA | 60.2 | 0.19 |
| mm4_exon_Col27a1_chr4_63214308_F | TCGTCGGCAGCGTCCTTTAGGGAAGGGGACTGCA | 59.0 | 0.18 |
| mm4_exon_Col27a1_chr4_63214308_R | GTCTCGTGGGCTCGGGTGAGTCCCAGGGCACTTAC | 60.0 | 0.18 |
| mm4_intergenic_Irak1/Mir5132/Mir718|Mecp2_chrX_74023940_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Irak1/Mir5132/Mir718|Mecp2_chrX_74023940_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_exon_Mamstr_chr7_45643029_F | TCGTCGGCAGCGTCGACCAGAAACTCAAGCCCCA | 59.8 | 0.17 |
| mm4_exon_Mamstr_chr7_45643029_R | GTCTCGTGGGCTCGGGGGGTGTCTCTGCAGATCAC | 60.1 | 0.17 |
| mm4_exon_Cdkn1c_chr7_143460230_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Cdkn1c_chr7_143460230_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intron_Mtmr4_chr11_87595487_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intron_Mtmr4_chr11_87595487_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Gm7094_chr1_21272376_F | TCGTCGGCAGCGTCTGGATATAGCAACCGCCTGC | 60.2 | 0.16 |
| mm4_exon_Gm7094_chr1_21272376_R | GTCTCGTGGGCTCGGAAGTTATGCGCTGGCTGGAT | 60.1 | 0.16 |
| mm4_exon_Cetn4_chr3_37312362_F | TCGTCGGCAGCGTCCTGGGAAGAACACCAGGGTC | 59.9 | 0.16 |
| mm4_exon_Cetn4_chr3_37312362_R | GTCTCGTGGGCTCGGGCTATAGCAACCGCCTGTCG | 61.2 | 0.16 |
| mm4_exon_Tcf15_chr2_152143769_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Tcf15_chr2_152143769_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Gm14616_chr2_174861447_F | TCGTCGGCAGCGTCGGCATGGGTCAAGGAGAACT | 59.6 | 0.15 |
| mm4_intron_Gm14616_chr2_174861447_R | GTCTCGTGGGCTCGGGGCTCTTGTCTGCCTTTCCA | 60.2 | 0.15 |
| mm4_exon_Pmepa1_chr2_173276210_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Pmepa1_chr2_173276210_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intergenic_Brd2|Gm20493_chr17_34122650_F | TCGTCGGCAGCGTCACGAAACACATAGCGCATGC | 59.9 | 0.15 |
| mm3_intergenic_Brd2|Gm20493_chr17_34122650_R | GTCTCGTGGGCTCGGGCCACACCCATCGCTCAA | 60.3 | 0.15 |
| mm4_intron_Zfp598_chr17_24669987_F | TCGTCGGCAGCGTCGTGTGCTACCGGTGCTCTAC | 60.4 | 0.14 |
| mm4_intron_Zfp598_chr17_24669987_R | GTCTCGTGGGCTCGGGCAAAGCCATCAGGAGTCCG | 61.3 | 0.14 |
| mm4_intron_Phactr2_chr10_13285352_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Phactr2_chr10_13285352_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm26887|Mir23a_chr8_84200981_F | TCGTCGGCAGCGTCGGAATTGGGGTCCGACTCG | 60.1 | 0.13 |
| mm4_intergenic_Gm26887|Mir23a_chr8_84200981_R | GTCTCGTGGGCTCGGCAGATCCAGTGCTTGCCTCC | 60.7 | 0.13 |
| mm4_intergenic_Bean1|Gm10631_chr8_104171007_F | TCGTCGGCAGCGTCCCGCAGCCAAGCCGAGTA | 62.4 | 0.11 |
| mm4_intergenic_Bean1|Gm10631_chr8_104171007_R | GTCTCGTGGGCTCGGGCATAAGCAGCGGTACCCC | 60.8 | 0.11 |
| mm4_exon_Reps1_chr10_18055890_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Reps1_chr10_18055890_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Midn_chr10_80148724_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Midn_chr10_80148724_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Adamtsl5_chr10_80345269_F | TCGTCGGCAGCGTCACAGAGCATGTGTAGGCAGG | 59.7 | 0.10 |
| mm4_exon_Adamtsl5_chr10_80345269_R | GTCTCGTGGGCTCGGAGGTGTTCCAGCTCTTGTGG | 59.8 | 0.10 |
| mm4_exon_Kdm5b_chr1_134560319_F | TCGTCGGCAGCGTCGGAGCCTGCCCTGTGCTC | 62.7 | 0.10 |
| mm4_exon_Kdm5b_chr1_134560319_R | GTCTCGTGGGCTCGGGGAGGCAGGAACTCTCCCA | 60.6 | 0.10 |
| mm4_exon_Ankrd13b_chr11_77489576_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Ankrd13b_chr11_77489576_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_exon_Pde3b_chr7_114415949_F | TCGTCGGCAGCGTCGCCTGTGCCTTCTTCTTCCT | 59.9 | 0.10 |
| mm3_exon_Pde3b_chr7_114415949_R | GTCTCGTGGGCTCGGGAAGTCGCCCAGGTAGCAG | 60.1 | 0.10 |
| mm4_exon_Gm17110/Bmpr1a_chr14_34502249_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Gm17110/Bmpr1a_chr14_34502249_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Hnrnph3_chr10_63023755_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Hnrnph3_chr10_63023755_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Arx|Pola1_chrX_93299098_F | TCGTCGGCAGCGTCTTTTACTCCCTGGGCTCACC | 59.3 | 0.09 |
| mm4_intergenic_Arx|Pola1_chrX_93299098_R | GTCTCGTGGGCTCGGCACCCTGGGAGCATTGGC | 60.7 | 0.09 |
| mm4_exon_Socs7_chr11_97363307_F | TCGTCGGCAGCGTCCTGTTTCGCACGAAGAGCTG | 59.8 | 0.09 |
| mm4_exon_Socs7_chr11_97363307_R | GTCTCGTGGGCTCGGCTGAAGCTGCCAGATCTCCC | 60.1 | 0.09 |
| mm4_exon_Fam160b2_chr14_70599806_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Fam160b2_chr14_70599806_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Smyd2|Prox1_chr1_189922414_F | TCGTCGGCAGCGTCGAACTCGGCGCTCTGCTC | 60.8 | 0.08 |
| mm4_intergenic_Smyd2|Prox1_chr1_189922414_R | GTCTCGTGGGCTCGGCTCCAGCCTTCCTCACCG | 59.4 | 0.08 |
| mm4_intron_Fstl5_chr3_76604941_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Fstl5_chr3_76604941_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Phip_chr9_82975061_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Phip_chr9_82975061_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Cwc27_chr13_104767677_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Cwc27_chr13_104767677_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Olfr585|Olfr586_chr7_103101343_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Olfr585|Olfr586_chr7_103101343_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Olfr1271|Olfr1272_chr2_90272868_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Olfr1271|Olfr1272_chr2_90272868_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm27025|Gm25700_chr13_86690554_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm27025|Gm25700_chr13_86690554_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm15659|Gm8812_chr1_66048698_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm15659|Gm8812_chr1_66048698_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm14940|Gm17521_chrX_122862615_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm14940|Gm17521_chrX_122862615_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Epha4|Gm26263_chr1_77783532_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Epha4|Gm26263_chr1_77783532_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Dcx|Gm15048_chrX_143978976_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Dcx|Gm15048_chrX_143978976_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Osbp_chr19_11966099_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Osbp_chr19_11966099_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Phlpp1_chr1_106172914_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Phlpp1_chr1_106172914_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Sptbn1|Gm8098_chr11_30268236_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Sptbn1|Gm8098_chr11_30268236_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm13203|Casz1_chr4_148804297_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm13203|Casz1_chr4_148804297_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Atp11c|Gm7073_chrX_60404070_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Atp11c|Gm7073_chrX_60404070_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm13134|Prdm16_chr4_154636667_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm13134|Prdm16_chr4_154636667_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Kcmf1_chr6_72899491_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Kcmf1_chr6_72899491_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Btbd11_chr10_85386879_F | TCGTCGGCAGCGTCCCTGGCTACAGCGCTTTAGT | 60.1 | 0.06 |
| mm4_exon_Btbd11_chr10_85386879_R | GTCTCGTGGGCTCGGTAGGACCTAGATCGCCCCAG | 59.8 | 0.06 |
| mm4_intron_Arhgap21_chr2_20968220_F | TCGTCGGCAGCGTCAAAAGCCAGCTCGGACCAG | 60.3 | 0.05 |
| mm4_intron_Arhgap21_chr2_20968220_R | GTCTCGTGGGCTCGGAGGAAAACAGCCGTCTCCTT | 59.2 | 0.05 |
| mm4_intergenic_A330074K22Rik|Gse1_chr8_120228639_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_A330074K22Rik|Gse1_chr8_120228639_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Rab12_chr17_66519597_F | TCGTCGGCAGCGTCCTGGGATCCATGCACGCATA | 60.2 | 0.05 |
| mm4_exon_Rab12_chr17_66519597_R | GTCTCGTGGGCTCGGGGAGAAGCTGCTTCCCTTCC | 60.3 | 0.05 |
| mm4_exon_9930021J03Rik_chr19_29805747_F | TCGTCGGCAGCGTCTTCCTCCTCCTCGTCCACC | 60.3 | 0.05 |
| mm4_exon_9930021J03Rik_chr19_29805747_R | GTCTCGTGGGCTCGGGAACGAGGAAGAGGTGGTGG | 60.0 | 0.05 |
| mm4_intergenic_Commd3|Bmi1_chr2_18676934_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Commd3|Bmi1_chr2_18676934_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Homer3_chr8_70283043_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Homer3_chr8_70283043_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Bves_chr10_45335854_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Bves_chr10_45335854_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Ccdc178|Asxl3_chr18_22344414_F | TCGTCGGCAGCGTCAAGAAGAAGGACCGCACCTG | 59.9 | 0.04 |
| mm4_intergenic_Ccdc178|Asxl3_chr18_22344414_R | GTCTCGTGGGCTCGGCGCGGAATAACAAAGAGCCG | 59.9 | 0.04 |
| mm4_exon_4932427H20Rik/Snx14_chr9_88438934_F | TCGTCGGCAGCGTCCTAGACTCAGCCGCACAAGG | 60.4 | 0.04 |
| mm4_exon_4932427H20Rik/Snx14_chr9_88438934_R | GTCTCGTGGGCTCGGCAGAACGTCAGTCCGAGCG | 60.7 | 0.04 |
| mm4_exon_Actg1/mmu-mir-6935_chr11_120347910_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Actg1/mmu-mir-6935_chr11_120347910_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Adam9_chr8_24954542_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Adam9_chr8_24954542_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Fam135a_chr1_24100010_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Fam135a_chr1_24100010_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Bend6|Dst_chr1_33908188_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Bend6|Dst_chr1_33908188_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Vrk1_chr12_106010511_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Vrk1_chr12_106010511_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Usf2_chr7_30956649_F | TCGTCGGCAGCGTCGAGGCCGCTCGGGATCAT | 61.6 | 0.03 |
| mm4_exon_Usf2_chr7_30956649_R | GTCTCGTGGGCTCGGGTCTGGATCCCGCTTCCTC | 59.5 | 0.03 |
| mm4_intergenic_4930555K19Rik|Oxr1_chr15_41447412_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_4930555K19Rik|Oxr1_chr15_41447412_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Rnf130_chr11_50025423_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Rnf130_chr11_50025423_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24635|Arrdc3_chr13_80883136_F | TCGTCGGCAGCGTCTCTGCTCAGCTCCGGGAG | 60.7 | 0.03 |
| mm4_intergenic_Gm24635|Arrdc3_chr13_80883136_R | GTCTCGTGGGCTCGGCATGGGCCGCTGCGATTG | 62.2 | 0.03 |
| mm4_intron_Casd1_chr6_4601310_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Casd1_chr6_4601310_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Agfg2_chr5_137684532_F | TCGTCGGCAGCGTCCTTCAGCCTCCGCTTTGCTC | 61.6 | 0.02 |
| mm4_exon_Agfg2_chr5_137684532_R | GTCTCGTGGGCTCGGGAGGAGAGATGGTGATGGCG | 59.9 | 0.02 |
| mm4_intergenic_Gm15668|Kif22-ps_chr1_67543037_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm15668|Kif22-ps_chr1_67543037_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Mtg2|Hrh3/Gm17180_chr2_180093566_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Mtg2|Hrh3/Gm17180_chr2_180093566_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm15668|Kif22-ps_chr1_67542836_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm15668|Kif22-ps_chr1_67542836_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Atxn7l3_chr11_102296519_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Atxn7l3_chr11_102296519_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Naa15_chr3_51416553_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Naa15_chr3_51416553_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Smad3_chr9_63757403_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Smad3_chr9_63757403_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm15830|Hopx_chr5_77114645_F | TCGTCGGCAGCGTCGCAACAGCACTCTGACTCCT | 59.9 | 0.02 |
| mm4_intergenic_Gm15830|Hopx_chr5_77114645_R | GTCTCGTGGGCTCGGACAACGGTGGAGGTGAAAGG | 60.1 | 0.02 |
| mm4_intergenic_Rhox4f|Rhox3g_chrX_37617959_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Rhox4f|Rhox3g_chrX_37617959_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Olfr135|Olfr138_chr17_38225252_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Olfr135|Olfr138_chr17_38225252_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slc27a3|Ints3_chr3_90389991_F | TCGTCGGCAGCGTCCCTTCCCACCCAGCTTCTG | 60.0 | 0.02 |
| mm4_intergenic_Slc27a3|Ints3_chr3_90389991_R | GTCTCGTGGGCTCGGGTCAAATTCCGGAGCGTGTG | 59.8 | 0.02 |
| mm3_exon_Satb1_chr17_51813548_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_exon_Satb1_chr17_51813548_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Klf11_chr12_24651355_F | TCGTCGGCAGCGTCGCAGGGAGCGGTGTATTTTG | 59.5 | 0.02 |
| mm4_exon_Klf11_chr12_24651355_R | GTCTCGTGGGCTCGGCACCTCCGCCTTCCTTGC | 60.7 | 0.02 |
| mm4_intergenic_Gm26037|Agap1_chr1_89703480_F | TCGTCGGCAGCGTCGCTCTGACAGCCAGCACTTA | 60.0 | 0.01 |
| mm4_intergenic_Gm26037|Agap1_chr1_89703480_R | GTCTCGTGGGCTCGGCTGATTGTGGAGCCTCCCAG | 60.1 | 0.01 |
| mm4_intergenic_Gm16287|Capzb_chr4_139192754_F | TCGTCGGCAGCGTCGACAGGACACGCCCACTC | 60.0 | 0.01 |
| mm4_intergenic_Gm16287|Capzb_chr4_139192754_R | GTCTCGTGGGCTCGGCAGCTCCCGACCAATGAGAG | 60.1 | 0.01 |
| mm4_exon_Ptger3_chr3_157567607_F | TCGTCGGCAGCGTCCTTCATCAGCACCGGGCC | 60.8 | 0.01 |
| mm4_exon_Ptger3_chr3_157567607_R | GTCTCGTGGGCTCGGCTTTGATGGTCGCCAGGTTG | 59.4 | 0.01 |
| mm4_intron_Tfeb_chr17_47760502_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Tfeb_chr17_47760502_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Nptx2_chr5_144546401_F | TCGTCGGCAGCGTCACAGGCAAGGACACCATGG | 59.9 | 0.01 |
| mm4_exon_Nptx2_chr5_144546401_R | GTCTCGTGGGCTCGGACAGGTACCTCGAGGCTCTC | 60.3 | 0.01 |
| mm4_intron_Sox9_chr11_112783140_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Sox9_chr11_112783140_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Scn11a_chr9_119815835_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Scn11a_chr9_119815835_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fam20c_chr5_138792856_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fam20c_chr5_138792856_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Cebpb/Tmem189_chr2_167689912_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Cebpb/Tmem189_chr2_167689912_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Fam102b_chr3_109027397_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Fam102b_chr3_109027397_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Pdgfb_chr15_80014582_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Pdgfb_chr15_80014582_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Gse1_chr8_120536494_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Gse1_chr8_120536494_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Akap10_chr11_61930012_F | TCGTCGGCAGCGTCTACCTTTCCGCCGGAAGAAG | 59.7 | 0.00 |
| mm4_exon_Akap10_chr11_61930012_R | GTCTCGTGGGCTCGGCAGCTGCTGAATGAGGGGAG | 60.4 | 0.00 |
| mm4_exon_Mrpl20_chr4_155803756_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Mrpl20_chr4_155803756_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hao1_chr2_134527876_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hao1_chr2_134527876_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Chrdl2|Pold3_chr7_100042177_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Chrdl2|Pold3_chr7_100042177_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm17655|Plcxd1_chr5_110094221_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm17655|Plcxd1_chr5_110094221_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hnrnpr_chr4_136311405_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hnrnpr_chr4_136311405_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Il11_chr7_4776975_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Il11_chr7_4776975_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zfp524_chr7_5015573_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zfp524_chr7_5015573_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_A930011O12Rik/Mir124a-1/Mir3078|Kif13b_chr14_64629462_F | TCGTCGGCAGCGTCTGGGTGTTGTGTTGTGTCGT | 60.3 | 0.00 |
| mm4_intergenic_A930011O12Rik/Mir124a-1/Mir3078|Kif13b_chr14_64629462_R | GTCTCGTGGGCTCGGACACAGTTGGTCTTGTCCCC | 59.8 | 0.00 |
| mm4_intron_Chd1_chr17_15705462_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Chd1_chr17_15705462_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_B330016D10Rik_chr4_141546694_F | TCGTCGGCAGCGTCCCGGCTGCCATTTTGTAGC | 59.8 | 0.00 |
| mm4_exon_B330016D10Rik_chr4_141546694_R | GTCTCGTGGGCTCGGCCCCAGGCGGAGAACAAA | 59.5 | 0.00 |
| mm4_intron_Rc3h1_chr1_160906907_F | TCGTCGGCAGCGTCGGTAGGTAGGCCGAGGGG | 60.5 | 0.00 |
| mm4_intron_Rc3h1_chr1_160906907_R | GTCTCGTGGGCTCGGGAACCAGTCAGCCTCGGAC | 60.0 | 0.00 |
| mm4_intergenic_Tmem229b|Plekhh1_chr12_79029100_F | TCGTCGGCAGCGTCGTCCAGTCTGGTGAGCATCC | 60.1 | 0.00 |
| mm4_intergenic_Tmem229b|Plekhh1_chr12_79029100_R | GTCTCGTGGGCTCGGAGGAAATGACCTCAGCAGCC | 60.0 | 0.00 |
| mm4_intergenic_Samd4b|Gmfg_chr7_28436050_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Samd4b|Gmfg_chr7_28436050_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cgnl1|RP23-79K5.1_chr9_71771661_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cgnl1|RP23-79K5.1_chr9_71771661_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Tex261_chr6_83775725_F | TCGTCGGCAGCGTCCCAGCTCAGCACGTACATGA | 60.1 | 0.00 |
| mm4_exon_Tex261_chr6_83775725_R | GTCTCGTGGGCTCGGCTCCTCTCGGCGTCCTCT | 60.1 | 0.00 |
| mm4_exon_Fam105b_chr15_27630506_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Fam105b_chr15_27630506_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_B3gat3_chr19_8920463_F | TCGTCGGCAGCGTCTAGTAAGGGAGCCTGGGGAA | 59.2 | 0.00 |
| mm4_exon_B3gat3_chr19_8920463_R | GTCTCGTGGGCTCGGTCAGCTTCATGGCCGGTG | 60.0 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm0_exon_Pop5_chr5_115238090 |
TGCGGTTCAAGCACAGGTAAGCAGCGCTGTCCCCGCGCCGTCCCCGCGACCCGGGCCGCGCTCACCCCGCTGTCGCCGCA GGTACCTTCTGTGCGAG |
| mm4_intron_Sec24b_chr3_129993189 |
TGTGCATTGAGAGCCCTGAGGGGAAAGGGGATGGGCTTGAGACAGCACGAGCAACACCCTCCCCGCAGCCCGAGCCGCTG GCCGATGCTCTAACTAAACAAACCCACCCGTTCTCTAAAGGGAGCC |
| mm4_intron_Fam65a_chr8_105605471 |
GACTTGCGACCGGGTCCCGCCTCACCCCGTTCTCGCGACCCGGATCGCGCCGTGTCTTTTCTNNNNNNNNNNNNNNNNNN NNNGGGCACGGAGAACGCAGCTTCG |
| mm4_intron_Dgkd_chr1_87853608 |
TCCTGCGGTCGCTGCTACGGGGCCGAGCGCGCCGCGGCTTAACGGACCGCGGGGACCGGGCCGCGAGGACGGGGCCGCGG GGACCGGTCTGCGGGGACTGGGCGCAGGTGGGCGCCGCCGCTGAGGAGACCGAGGCCC |
| mm3_exon_Dclk2_chr3_86920092 |
CTTGGGGCCAGAGATGCTGCTGCCCCCGGAGGAGCTGGGAGCCCCTCTCCGTGACCCTGGCCGCGGCCTTTTGTCCCGTT CTTCAAAATGTTCCAGCTCAATGCTCCTTGTGCTG |
| mm4_intron_Tardbp_chr4_148626599 |
CTTAGGCCTTAACGACGCGGCTGCGCGAGGCCAACCAGGCCCCGCTCTACCGCTCCCTCCCCGCGGCGCAGGCCGCACCG GGCCTCTCCGAAGCAACACTCGA |
| mm4_exon_Sms_chrX_157492095 |
CCAGGAGTTAGGAGGTGGGAGGGGCCGCGGGCAAGCGANNNNNNNNNNNNNNNNNNNNNNNACCCGCCTCTTAGAGGAGG AAGCAGGGACG |
| mm3_intergenic_Klf10|Gm26863_chr15_38316306 |
ACCCAACACTCCTACCCAGATGGAATAAAAAGACCCTTCCCCGCAACCCTGGCCGAGGGAGAGTGACTGAAGGCGAAGGT G |
| mm3_intron_Agpat5_chr8_18846810 |
CGGCTTTACTGCGTCTACCAGAACATGGTGCTCTTCTTCTTCGAGAACTACACCGGGGTCCAGGTGAGGCGCGGCCGCGC AGGGCTGCGTGCGAGCCCTCCCCGCGGCCGGGGCGGCGCTTGCAACCCGGGCGAACACTC |
| mm4_exon_Abcb6_chr1_75180222 |
TCACTGCACAAAGAGTCGGGCTGACCCTTCAGAGAGGGCCGCAGATGCCAGGGTGTCCTGGGACATGGAGCTACGCCGCT TGGGTCGCGGGAAGGGACGCACGTGGACCTGCTACACTGCCCACTCTC |
| mm3_intergenic_Pde7a|Gm22282_chr3_19311575 |
GTCCGGACAGGGCTGCTCGCGTCCGCTGCGCTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCCCCCCGGCCCCTCC CGCCTCCCCGCAGCTCCCTCCCCGCAACCCGCGGCGCGTGCGCAGGAGAGTTACGTG |
| mm4_intron_Ubp1_chr9_113931795 |
GCGCCTACAGCATGAGGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGGGGTCCTGCCGCG TGGCGCCGTGGCCTGGGAGGCCGACGGGGGCGGCTAGCGGCGGAGGACCCCGTCGCTTCT |
| mm4_intron_Sh3bp4_chr1_89070766 |
TTCCCTGCCCTCTGTGGTCACAGCCCGACCTGGGCTGCGGATCGTGGTCGCTANNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNTTCTGAGAAGGAGCGAAGAGAGCG |
| mm4_intron_Tbx1_chr16_18586502 |
CTTCATCCGCTTTTCAGCCGGGCTCCTGCGCATCCCTGACACGCAGTCAGAGGCACAGAACGCACGTGGACAGGGGCGCG GGGAAGGGGCGCCTGACCTGCCTGCCTTGGTGACGATCAT |
| mm4_intergenic_Gm5449|Drd1a_chr13_53639994 |
GTGTGGTAGGAGGCAGGAACTGCAGGCGTTCAGGCTCCAGGTGCACACACAGAGGTCTGTAGCTGGTGTCTGGAGTGACA CAGGCCGGGGCGCGGGGAAGGGTTATGGGCTCAGGAGGTTGGT |
| mm4_exon_Col27a1_chr4_63214308 |
CTTTAGGGAAGGGGACTGCANNNNNNNNNNNNNNNNNNNNNNTGCGGGGACCCATGACCACAGATGCCGTTAGGCACCCG CCTAAGAACCGACCGGCCAGCGTCACACTTGGGGACCCAGTAAGTGCCCTGGGACTCAC |
| mm4_exon_Mamstr_chr7_45643029 |
GACCAGAAACTCAAGCCCCATTCTCCTCCCTCAAACCTACAGTGCAAAGAAGAGAAGCTTGACCTGGAACCTCCTCCCCC GGGACCCCGGCCGTGCATGCTCCCCGTGATCTGCAGAGACACCCC |
| mm4_exon_Gm7094_chr1_21272376 |
TGGATATAGCAACCGCCTGCCGGGAGCAAACGCGCGCCCTGGAGGGGAGAGCGGCTGTTGCGCGGGCGCGGGCAGGGGCG CAGGGAGGGGATATGGCATCCAGCCAGCGCATAACTT |
| mm4_exon_Cetn4_chr3_37312362 |
CTGGGAAGAACACCAGGGTCCCACAGCACTCACCATATCCCCTCCCTGCGCCCCTGCCCGCGCCCGCGCAACAGCCGCTC TCCCCTCCGCGGCCCGCGTTTGCTCCCGACAGGCGGTTGCTATAGC |
| mm4_intron_Gm14616_chr2_174861447 |
GGCATGGGTCAAGGAGAACTCGGGGCCTGGGTAGCGAGGAAGGTTTCATTGGATGACATAATTGACTGTGGAAAGGCAGA CAAGAGCC |
| mm3_intergenic_Brd2|Gm20493_chr17_34122650 |
ACGAAACACATAGCGCATGCGCAAGGGTGTGCCTGGTCTGGGGGGAGGGGAGGCACGGGTCGCGGGGAGAGCCGCGGTGC GCAGGCTCAGTGCGCCCCGAGCCTCGGGGCCACCTCGTTGAGCGATGGGTGTGGC |
| mm4_intron_Zfp598_chr17_24669987 |
GTGTGCTACCGGTGCTCTACCAAGATGCGGGTGCTGTGCGAGCAGCGCTACTGCGCCGTGTGCCGCGAGGAGCTGCGCCA GGTGCGCCAGCGGGGCGCGCGACGGGGCGCGGGGAGGGCGGACTCCTGATGGCTTTGC |
| mm4_intergenic_Gm26887|Mir23a_chr8_84200981 |
GGAATTGGGGTCCGACTCGGGAGAGACTCGCGGGGCGCGGGCGGCGACAACTTTCCCGCGCGGCCCGGGGAGCAGAGAGG GGGCCGTGACCGCCTGTCCCGGGAGGCAAGCACTGGATCTG |
| mm4_intergenic_Bean1|Gm10631_chr8_104171007 | CCGCAGCCAAGCCGAGTAAGGGGCGCGGGGCGCGGGGAGGGCCGCAGACGGGGGTACCGCTGCTTATGC |
| mm4_exon_Adamtsl5_chr10_80345269 |
ACAGAGCATGTGTAGGCAGGCAGAGGGCGCGGCCCGGCTTGCGGCNNNNNNNNNNNNNNNNNNNNNATTCCCGGGTTACC GGACACATTGGCGTCTGCGCACTGAGACGCCCCGACCACAAGAGCTGGAACACCT |
| mm4_exon_Kdm5b_chr1_134560319 |
GGAGCCTGCCCTGTGCTCGCCCCTGCCCAGGTCGCGGTGATGGAGCCGGCCACCACGCTGCCCCCANNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNTGGGAGAGTTCCTGCCTCC |
| mm3_exon_Pde3b_chr7_114415949 |
GCCTGTGCCTTCTTCTTCCTCACCTGTTTCCTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCTGGTGGCT GCTGGCGCTGCCCGCCTGCTGCTACCTGGGCGACTTC |
| mm4_intergenic_Arx|Pola1_chrX_93299098 |
TTTTACTCCCTGGGCTCACCCCTCCTGCGGCGGGAACCGTAGCTCCCCCCTTCTCCGGGACCCTGCCCGCGCCCCTTCGG GTGACCGCTCGGTGGAAGCTCGCCCGCGGGGGGCTGGGTCTCGCCAATGCTCCCAGGGTG |
| mm4_exon_Socs7_chr11_97363307 |
CTGTTTCGCACGAAGAGCTGTAACGGCGGCTCGGGCGGCGGGGATGGGACCGGCAAGAGGCCTTCGGGAGATCTGGCAGC TTCAG |
| mm4_intergenic_Smyd2|Prox1_chr1_189922414 |
GAACTCGGCGCTCTGCTCGGGGCGGGTGCTGTGCCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNACGGGAGTCCGCCTGCACCCGCCAGGCTGCGCTGCCCGGTGAGGAAGGCTGGAG |
| mm4_exon_Btbd11_chr10_85386879 |
CCTGGCTACAGCGCTTTAGTGCGGGGCGGGAGCTCAAGAGAACCGCGGCCCGGATCCCGGAGCCGGGGGAGCCCGTAGCC CGCCGAGCTGCGGCGGCCGTATCTGGGGCGATCTAGGTCCTA |
| mm4_intron_Arhgap21_chr2_20968220 |
AAAAGCCAGCTCGGACCAGGGCGGGCGTGGGCTCGGCGGCGTCTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGGAGACGGCTGTTTTCCT |
| mm4_exon_Rab12_chr17_66519597 |
CTGGGATCCATGCACGCATACGGCTCGCGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCTCCACCCGCTC CGNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGTAGAGGAAGGGAAGCAGCTTCTCC |
| mm4_exon_9930021J03Rik_chr19_29805747 |
TTCCTCCTCCTCGTCCACCGAGGAGGCACTCCTCCGGCGGACCAGCCCCGAGGCCCGGGCCGCCGCTGCCACCACCACCT CTTCCTCGTTC |
| mm4_intergenic_Ccdc178|Asxl3_chr18_22344414 |
AAGAAGAAGGACCGCACCTGGGCCGAGGCTGCCCGCCTGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTCCGGCTCTTTGTTATTCCGCG |
| mm4_exon_4932427H20Rik/Snx14_chr9_88438934 |
CTAGACTCAGCCGCACAAGGCCCCGCCCCGCAACCCTCGCCGCGCGTGCGCAACCAACGCCCCAACCACACGCAAGGCCG CCCCAAGGCCGCCGCCACCATCCCGCGGGAGGACTGGGGAGCGCTCGGACTGACGTTCTG |
| mm4_exon_Usf2_chr7_30956649 |
GAGGCCGCTCGGGATCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCT GTCGGCGCGCTTACCTGGCGGCGGCAGCAGCGGTGGCCGAGGAAGCGGGATCCAGAC |
| mm4_intergenic_Gm24635|Arrdc3_chr13_80883136 |
TCTGCTCAGCTCCGGGAGCGCGTGCGGGGCCGGCGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNTGCCCGCCTGCCAATCGCAGCGGCCCATG |
| mm4_exon_Agfg2_chr5_137684532 | CTTCAGCCTCCGCTTTGCTCCCTCCGACCCCGCCACCCGGGCCCGGGCCCTTCTTAGCCGCCATCACCATCTCTCCTC |
| mm4_intergenic_Gm15830|Hopx_chr5_77114645 |
GCAACAGCACTCTGACTCCTCGCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCAGGCAAGCAGGCAAGGGGGTAAGA ATTAATTTTTTTCTCCCTTTCACCTCCACCGTTGT |
| mm4_intergenic_Slc27a3|Ints3_chr3_90389991 |
CCTTCCCACCCAGCTTCTGCGGTGTGGCGCGGGAGCCAGGAGTGCGAGAGTCGGGGGCGGCCCGGCCCTCCCCACAACCC AGGCCCCGCCCTAGGCGCACACGCTCCGGAATTTGAC |
| mm4_exon_Klf11_chr12_24651355 |
GCAGGGAGCGGTGTATTTTGGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNAGGCAGAGCCGCGCGGCAAGGAAGGCGGAGGTG |
| mm4_intergenic_Gm26037|Agap1_chr1_89703480 |
GCTCTGACAGCCAGCACTTATCTCGGGCCTCCGGAGCCCGTGCCCATCAGCCCTCCCCCTGGCCAGGGCCGCGGGATGGT CAGCCGAGCCTCACATTGTACTGGGAGGCTCCACAATCAG |
| mm4_intergenic_Gm16287|Capzb_chr4_139192754 |
GACAGGACACGCCCACTCGACCACAGGGCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCATACTTGGCGATCGCTGC GGCCCCGCGCTCTCATTGGTCGGGAGCTG |
| mm4_exon_Ptger3_chr3_157567607 |
CTTCATCAGCACCGGGCCGGCGGGCAACGAGACAGACCCTGCGCGCGAGCCGGGCAGCGTGGCCTTTGCCTCCGCCTTCG CCTGCTTGGGCTTGCTGGCTCTGGTGGTGACCTTTGCCTGCAACCTGGCGACCATCAAAG |
| mm4_exon_Nptx2_chr5_144546401 |
ACAGGCAAGGACACCATGGGCGACCTGCCGCGGGACCCGGGCCACGTCGTGGAGCAGCTTAGCCGCTCCTTGCAAACCCT CAAGGACCGCTTGGAGAGCCTCGAGGTACCTGT |
| mm4_exon_Akap10_chr11_61930012 |
TACCTTTCCGCCGGAAGAAGGACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTGTCGCGGGGAGGGCCCG GCTCCCCTCATTCAGCAGCTG |
| mm4_intergenic_A930011O12Rik/Mir124a-1/Mir3078|Kif13b_chr14_64629462 |
TGGGTGTTGTGTTGTGTCGTTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTACAGGGCC CGGGTGGGGGGGAGGGGAGGGGGACAAGACCAACTGTGT |
| mm4_exon_B330016D10Rik_chr4_141546694 |
CCGGCTGCCATTTTGTAGCGCGGCCGCCATTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGTTCTCCGCCTGGGG |
| mm4_intron_Rc3h1_chr1_160906907 |
GGTAGGTAGGCCGAGGGGCGGTGCGGACGCCGCCCTGGACGCCGGGAAGGCTGGGGAGGCTGGGGCGGGAGCGGGGGGTT GGGGGGGTCCGAGGCTGACTGGTTC |
| mm4_intergenic_Tmem229b|Plekhh1_chr12_79029100 |
GTCCAGTCTGGTGAGCATCCCGCCGGGGATCCCACGCTGTCTGGGGGTCGCAGCGCGCAGCCAGCCCTCCANNNNNNNNN NNNNNNNNNNNNNNNTGGGAGGCTGCGGCTGCTGAGGTCATTTCCT |
| mm4_exon_Tex261_chr6_83775725 |
CCAGCTCAGCACGTACATGAACCACATNNNNNNNNNNNNNNNNNNNNNACGGGCCTGCGCGGTCCGGCTCCCGCGACACT GAGCGGCCGCCGCCGTGCTGTCCCCGCCCAGAGGACGCCGAGAGGAG |
| mm4_exon_B3gat3_chr19_8920463 |
TAGTAAGGGAGCCTGGGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNA CCTGTCCCTGCCGGTCCCGGTGGACCCCGCCTGCCCCGGTGGCACCGGCCATGAAGCTGA |