CRISPOR

DNA break repair outcome predictions

Guide: CCCGGGTCGCGGGGACGGCG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGACAGCGGGGTGAGCGCGGCCCGGGTCGCGGGGACGGCGCGGGGACAGCGCTGCTTACCCGTGCTTGAACCGCACCATG`	(Wild-type sequence)
750	`GTGAGCGCGGCCCGGGTCGCGGGGAC-----------AGCGCTGCTTACCCGTGCTTGAA`	`11 bp deleted → frameshift`
467	`GTGAGCGCGGCCCGGGTCGCGGGGACGG-----GGACAGCGCTGCTTACCCGTGCTTGAA`	`5 bp deleted → frameshift`
364	`GTGAGCGCGGCCCGGGTCGCGGGG----------GACAGCGCTGCTTACCCGTGCTTGAA`	`10 bp deleted → frameshift`
362	`GTGAGCGCGGCCCGGGTCGCGGGGACGGCG--GGGACAGCGCTGCTTACCCGTGCTTGAA`	`2 bp deleted → frameshift`
346	`GTGAGCGCGGCCCGGGTCGCGGGGACGGCG-----------CTGCTTACCCGTGCTTGAA`	`11 bp deleted → frameshift`
329	`GTGAGCGCGGCCCGGGTCGCGGG------------ACAGCGCTGCTTACCCGTGCTTGAA`	`12 bp deleted → no frameshift`
325	`GTGAGCGCGGCCCGGG------------------GACAGCGCTGCTTACCCGTGCTTGAA`	`18 bp deleted → no frameshift`
296	`GTGAGCGCGGCCCGGGTCGCGGGGACGG------GACAGCGCTGCTTACCCGTGCTTGAA`	`6 bp deleted → no frameshift`
282	`GTGAGCGCGGCCCGGGTCGCGGGGACGG-------ACAGCGCTGCTTACCCGTGCTTGAA`	`7 bp deleted → frameshift`
255	`GTGAGCGCGGCCCGGGTCGCGGGG---------GGACAGCGCTGCTTACCCGTGCTTGAA`	`9 bp deleted → no frameshift`
232	`GTGAGCGCGGCCCGGG-------------------ACAGCGCTGCTTACCCGTGCTTGAA`	`19 bp deleted → frameshift`
220	`GTGAGCGCGGCCCGGGTCGCG--------------------CTGCTTACCCGTGCTTGAA`	`20 bp deleted → frameshift`
208	`GTGAGCGCGGCCCGGGTCGCGGGGACGGC-------------TGCTTACCCGTGCTTGAA`	`13 bp deleted → frameshift`
208	`GTGAGCGCGGCCCGGGTCGCGG-------------ACAGCGCTGCTTACCCGTGCTTGAA`	`13 bp deleted → frameshift`
208	`GTGAGCGCGGCCCGGGTCG-------------GGGACAGCGCTGCTTACCCGTGCTTGAA`	`13 bp deleted → frameshift`
199	`GTGAGCGCGGCCCGGGTCGC----------------------TGCTTACCCGTGCTTGAA`	`22 bp deleted → frameshift`
198	`GTGAGCGCGGCCCGGGTCGCGGGGACG--------------CTGCTTACCCGTGCTTGAA`	`14 bp deleted → frameshift`
190	`GTGAGCGCGG-----------------------GGACAGCGCTGCTTACCCGTGCTTGAA`	`23 bp deleted → frameshift`
179	`GTGAGCGCGGCCCGGGTCGCGGGGACGGC----------------TTACCCGTGCTTGAA`	`16 bp deleted → frameshift`
170	`GTGAGCGCGGCCCGGG-----------------GGACAGCGCTGCTTACCCGTGCTTGAA`	`17 bp deleted → frameshift`
164	`GTGAGCGC----------------------------------TGCTTACCCGTGCTTGAA`	`34 bp deleted → frameshift`
147	`GTGAGCGCGGCCCGG--------------------ACAGCGCTGCTTACCCGTGCTTGAA`	`20 bp deleted → frameshift`
133	`GTGAGCGCGGCCCGGGTCGCGGGGACGGCG----------------------TGCTTGAA`	`22 bp deleted → frameshift`
121	`GTGAGCGCG--------------------------------CTGCTTACCCGTGCTTGAA`	`32 bp deleted → frameshift`
120	`GTGAGCGCGG------------------------GACAGCGCTGCTTACCCGTGCTTGAA`	`24 bp deleted → no frameshift`
120	`GTGAGCGCGGCCCG--------------------------------------TGCTTGAA`	`38 bp deleted → frameshift`
114	`GTGAGCGCGGCCCGGGTCGCGGGGACG-------------------------TGCTTGAA`	`25 bp deleted → frameshift`
114	`GTGAGCGCGGCCCGGGTCGC-------------------------TTACCCGTGCTTGAA`	`25 bp deleted → frameshift`
114	`GTGAGCGCGG-------------------------ACAGCGCTGCTTACCCGTGCTTGAA`	`25 bp deleted → frameshift`
114	`GTGAGCG-------------------------GGGACAGCGCTGCTTACCCGTGCTTGAA`	`25 bp deleted → frameshift`
109	`GTGAGCGCGGCCCGGGTCGCGGGGACGGC--------------------------TTGAA`	`26 bp deleted → frameshift`
103	`GTGAGCGCGGCCCG---------------------------CTGCTTACCCGTGCTTGAA`	`27 bp deleted → no frameshift`
95	`GTGAGCGCGGCCCGGGTCGCGGGGAC-----------------------CCGTGCTTGAA`	`23 bp deleted → frameshift`
94	`GTGAGCGCGGC-----------------------------GCTGCTTACCCGTGCTTGAA`	`29 bp deleted → frameshift`
84	`GTGAGCGCGGCCCGGGTCGCG-------------------------------TGCTTGAA`	`31 bp deleted → frameshift`
84	`GTGAGCGCGGC-------------------------------TGCTTACCCGTGCTTGAA`	`31 bp deleted → frameshift`
76	`GTGAGCGCGGCCCGGGTCG---------------------------------TGCTTGAA`	`33 bp deleted → no frameshift`
73	`GTGAGCGCGGC----------------------------------TTACCCGTGCTTGAA`	`34 bp deleted → frameshift`
69	`GTGAGCGCGGCCCGGGTCGC-----------------------------------TTGAA`	`35 bp deleted → frameshift`
66	`GTGAGC------------------------------------TGCTTACCCGTGCTTGAA`	`36 bp deleted → no frameshift`
62	`GTGAGCGCGGCCC-------------------------------------CGTGCTTGAA`	`37 bp deleted → frameshift`
62	`GTGAGCGC-------------------------------------TTACCCGTGCTTGAA`	`37 bp deleted → frameshift`
60	`GTGA--------------------------------CAGCGCTGCTTACCCGTGCTTGAA`	`32 bp deleted → frameshift`
56	`GTGAGCGCGGCC---------------------------------------GTGCTTGAA`	`39 bp deleted → no frameshift`
56	`GTGAGC---------------------------------------TTACCCGTGCTTGAA`	`39 bp deleted → no frameshift`
54	`GTGAGCGCGGCCCGGGTCGCGGGGA----------------------------------A`	`34 bp deleted → frameshift`
49	`GTGAGCGCGGCCCGGGT------------------------------------GCTTGAA`	`36 bp deleted → no frameshift`
46	`GTGAGCGCG-------------------------------------------TGCTTGAA`	`43 bp deleted → frameshift`
44	`GTGAGCGCGGC--------------------------------------------TTGAA`	`44 bp deleted → frameshift`
42	`GTGAGCG---------------------------------------------TGCTTGAA`	`45 bp deleted → no frameshift`
39	`GTG---------------------------------------------------CTTGAA`	`51 bp deleted → no frameshift`
38	`GTG-----------------------------------------CTTACCCGTGCTTGAA`	`41 bp deleted → frameshift`
38	`GTGAGCGC-----------------------------------------------TTGAA`	`47 bp deleted → frameshift`
34	`GTGAGC-------------------------------------------------TTGAA`	`49 bp deleted → frameshift`
25	`GTGA-------------------------------------------------------A`	`55 bp deleted → frameshift`

Version 5.01 - Documentation - Contact us - Downloads/local installation - Citation - License