CRISPOR

DNA break repair outcome predictions

Guide: CCGGGTCGCGGGGACGGCGC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GACAGCGGGGTGAGCGCGGCCCGGGTCGCGGGGACGGCGCGGGGACAGCGCTGCTTACCCGTGCTTGAACCGCACCATGG`	(Wild-type sequence)
634	`TGAGCGCGGCCCGGGTCGCGGGGAC-----------AGCGCTGCTTACCCGTGCTTGAAC`	`11 bp deleted → frameshift`
461	`TGAGCGCGGCCCGGGTCGCGGGGACGGCGC-----------TGCTTACCCGTGCTTGAAC`	`11 bp deleted → frameshift`
364	`TGAGCGCGGCCCGGGTCGCGGGG----------GACAGCGCTGCTTACCCGTGCTTGAAC`	`10 bp deleted → frameshift`
329	`TGAGCGCGGCCCGGGTCGCGGG------------ACAGCGCTGCTTACCCGTGCTTGAAC`	`12 bp deleted → no frameshift`
311	`TGAGCGCGGCCCGGGTCGCGGGGACGG-----GGACAGCGCTGCTTACCCGTGCTTGAAC`	`5 bp deleted → frameshift`
296	`TGAGCGCGGCCCGGGTCGCGGGGACGG------GACAGCGCTGCTTACCCGTGCTTGAAC`	`6 bp deleted → no frameshift`
282	`TGAGCGCGGCCCGGGTCGCGGGGACGG-------ACAGCGCTGCTTACCCGTGCTTGAAC`	`7 bp deleted → frameshift`
255	`TGAGCGCGGCCCGGGTCGCGGGGACGGCGC---------GCTGCTTACCCGTGCTTGAAC`	`9 bp deleted → no frameshift`
255	`TGAGCGCGGCCCGGGTCGCGGGG---------GGACAGCGCTGCTTACCCGTGCTTGAAC`	`9 bp deleted → no frameshift`
244	`TGAGCGCGGCCCGGG------------------GACAGCGCTGCTTACCCGTGCTTGAAC`	`18 bp deleted → no frameshift`
232	`TGAGCGCGGCCCGGG-------------------ACAGCGCTGCTTACCCGTGCTTGAAC`	`19 bp deleted → frameshift`
220	`TGAGCGCGGCCCGGGTCGCG--------------------CTGCTTACCCGTGCTTGAAC`	`20 bp deleted → frameshift`
208	`TGAGCGCGGCCCGGGTCGCGGGGACGGC-------------TGCTTACCCGTGCTTGAAC`	`13 bp deleted → frameshift`
208	`TGAGCGCGGCCCGGGTCGCGG-------------ACAGCGCTGCTTACCCGTGCTTGAAC`	`13 bp deleted → frameshift`
199	`TGAGCGCGGCCCGGGTCGC----------------------TGCTTACCCGTGCTTGAAC`	`22 bp deleted → frameshift`
198	`TGAGCGCGGCCCGGGTCGCGGGGACGGCGC--------------TTACCCGTGCTTGAAC`	`14 bp deleted → frameshift`
198	`TGAGCGCGGCCCGGGTCGCGGGGACG--------------CTGCTTACCCGTGCTTGAAC`	`14 bp deleted → frameshift`
179	`TGAGCGCGGCCCGGGTCGCGGGGACGGC----------------TTACCCGTGCTTGAAC`	`16 bp deleted → frameshift`
170	`TGAGCGCGGCCCGGG-----------------GGACAGCGCTGCTTACCCGTGCTTGAAC`	`17 bp deleted → frameshift`
164	`TGAGCGC----------------------------------TGCTTACCCGTGCTTGAAC`	`34 bp deleted → frameshift`
147	`TGAGCGCGGCCCGG--------------------ACAGCGCTGCTTACCCGTGCTTGAAC`	`20 bp deleted → frameshift`
133	`TGAGCGCGGCCCGGGTCGCGGGGACGGCG----------------------TGCTTGAAC`	`22 bp deleted → frameshift`
126	`TGAGCGCGG-----------------------GGACAGCGCTGCTTACCCGTGCTTGAAC`	`23 bp deleted → frameshift`
121	`TGAGCGCG--------------------------------CTGCTTACCCGTGCTTGAAC`	`32 bp deleted → frameshift`
120	`TGAGCGCGGCCCGGGTCGCGGGGACGGCGC------------------------TTGAAC`	`24 bp deleted → no frameshift`
120	`TGAGCGCGG------------------------GACAGCGCTGCTTACCCGTGCTTGAAC`	`24 bp deleted → no frameshift`
120	`TGAGCGCGGCCCG--------------------------------------TGCTTGAAC`	`38 bp deleted → frameshift`
114	`TGAGCGCGGCCCGGGTCGCGGGGACG-------------------------TGCTTGAAC`	`25 bp deleted → frameshift`
114	`TGAGCGCGGCCCGGGTCGC-------------------------TTACCCGTGCTTGAAC`	`25 bp deleted → frameshift`
114	`TGAGCGCGG-------------------------ACAGCGCTGCTTACCCGTGCTTGAAC`	`25 bp deleted → frameshift`
109	`TGAGCGCGGCCCGGGTCGCGGGGACGGC--------------------------TTGAAC`	`26 bp deleted → frameshift`
103	`TGAGCGCGGCCCG---------------------------CTGCTTACCCGTGCTTGAAC`	`27 bp deleted → no frameshift`
95	`TGAGCGCGGCCCGGGTCGCGGGGAC-----------------------CCGTGCTTGAAC`	`23 bp deleted → frameshift`
94	`TGAGCGCGGC-----------------------------GCTGCTTACCCGTGCTTGAAC`	`29 bp deleted → frameshift`
84	`TGAGCGCGGCCCGGGTCGCG-------------------------------TGCTTGAAC`	`31 bp deleted → frameshift`
84	`TGAGCGCGGC-------------------------------TGCTTACCCGTGCTTGAAC`	`31 bp deleted → frameshift`
76	`TGAGCGCGGCCCGGGTCG---------------------------------TGCTTGAAC`	`33 bp deleted → no frameshift`
73	`TGAGCGCGGC----------------------------------TTACCCGTGCTTGAAC`	`34 bp deleted → frameshift`
69	`TGAGCGCGGCCCGGGTCGC-----------------------------------TTGAAC`	`35 bp deleted → frameshift`
66	`TGAGC------------------------------------TGCTTACCCGTGCTTGAAC`	`36 bp deleted → no frameshift`
62	`TGAGCGCGGCCC-------------------------------------CGTGCTTGAAC`	`37 bp deleted → frameshift`
62	`TGAGCGC-------------------------------------TTACCCGTGCTTGAAC`	`37 bp deleted → frameshift`
60	`TGA--------------------------------CAGCGCTGCTTACCCGTGCTTGAAC`	`32 bp deleted → frameshift`
56	`TGAGCGCGGCC---------------------------------------GTGCTTGAAC`	`39 bp deleted → no frameshift`
56	`TGAGC---------------------------------------TTACCCGTGCTTGAAC`	`39 bp deleted → no frameshift`
54	`TGAGCGCGGCCCGGGTCGCGGGGA----------------------------------AC`	`34 bp deleted → frameshift`
52	`TGAGCGCGGCCCGGGTCGCGGGGAC-----------------------------------`	`35 bp deleted → frameshift`
49	`TGAGCGCGGCCCGGGT------------------------------------GCTTGAAC`	`36 bp deleted → no frameshift`
46	`TGAGCGCG-------------------------------------------TGCTTGAAC`	`43 bp deleted → frameshift`
44	`TGAGCGCGGC--------------------------------------------TTGAAC`	`44 bp deleted → frameshift`
42	`TGAGCG---------------------------------------------TGCTTGAAC`	`45 bp deleted → no frameshift`
38	`TG-----------------------------------------CTTACCCGTGCTTGAAC`	`41 bp deleted → frameshift`
38	`TGAGCGC-----------------------------------------------TTGAAC`	`47 bp deleted → frameshift`
34	`TGAGC-------------------------------------------------TTGAAC`	`49 bp deleted → frameshift`
25	`TGA-------------------------------------------------------AC`	`55 bp deleted → frameshift`
23	`TG---------------------------------------------------CTTGAAC`	`51 bp deleted → no frameshift`

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