CRISPOR

DNA break repair outcome predictions

Guide: CGGGTCGCGGGGACGGCGCG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ACAGCGGGGTGAGCGCGGCCCGGGTCGCGGGGACGGCGCGGGGACAGCGCTGCTTACCCGTGCTTGAACCGCACCATGGC`	(Wild-type sequence)
519	`GAGCGCGGCCCGGGTCGCGGGGAC-----------AGCGCTGCTTACCCGTGCTTGAACC`	`11 bp deleted → frameshift`
461	`GAGCGCGGCCCGGGTCGCGGGGACGGCGC-----------TGCTTACCCGTGCTTGAACC`	`11 bp deleted → frameshift`
382	`GAGCGCGGCCCGGGTCGCGGGGACGGCGCG---------CTGCTTACCCGTGCTTGAACC`	`9 bp deleted → no frameshift`
329	`GAGCGCGGCCCGGGTCGCGGG------------ACAGCGCTGCTTACCCGTGCTTGAACC`	`12 bp deleted → no frameshift`
296	`GAGCGCGGCCCGGGTCGCGGGGACGG------GACAGCGCTGCTTACCCGTGCTTGAACC`	`6 bp deleted → no frameshift`
282	`GAGCGCGGCCCGGGTCGCGGGGACGG-------ACAGCGCTGCTTACCCGTGCTTGAACC`	`7 bp deleted → frameshift`
242	`GAGCGCGGCCCGGGTCGCGGGG----------GACAGCGCTGCTTACCCGTGCTTGAACC`	`10 bp deleted → frameshift`
232	`GAGCGCGGCCCGGG-------------------ACAGCGCTGCTTACCCGTGCTTGAACC`	`19 bp deleted → frameshift`
220	`GAGCGCGGCCCGGGTCGCG--------------------CTGCTTACCCGTGCTTGAACC`	`20 bp deleted → frameshift`
208	`GAGCGCGGCCCGGGTCGCGGGGACGGC-------------TGCTTACCCGTGCTTGAACC`	`13 bp deleted → frameshift`
208	`GAGCGCGGCCCGGGTCGCGG-------------ACAGCGCTGCTTACCCGTGCTTGAACC`	`13 bp deleted → frameshift`
199	`GAGCGCGGCCCGGGTCGC----------------------TGCTTACCCGTGCTTGAACC`	`22 bp deleted → frameshift`
198	`GAGCGCGGCCCGGGTCGCGGGGACGGCGC--------------TTACCCGTGCTTGAACC`	`14 bp deleted → frameshift`
198	`GAGCGCGGCCCGGGTCGCGGGGACG--------------CTGCTTACCCGTGCTTGAACC`	`14 bp deleted → frameshift`
179	`GAGCGCGGCCCGGGTCGCGGGGACGGC----------------TTACCCGTGCTTGAACC`	`16 bp deleted → frameshift`
164	`GAGCGC----------------------------------TGCTTACCCGTGCTTGAACC`	`34 bp deleted → frameshift`
162	`GAGCGCGGCCCGGG------------------GACAGCGCTGCTTACCCGTGCTTGAACC`	`18 bp deleted → no frameshift`
147	`GAGCGCGGCCCGGGTCGCGGGGACGGCGCG--------------------TGCTTGAACC`	`20 bp deleted → frameshift`
147	`GAGCGCGGCCCGG--------------------ACAGCGCTGCTTACCCGTGCTTGAACC`	`20 bp deleted → frameshift`
133	`GAGCGCGGCCCGGGTCGCGGGGACGGCG----------------------TGCTTGAACC`	`22 bp deleted → frameshift`
121	`GAGCGCG--------------------------------CTGCTTACCCGTGCTTGAACC`	`32 bp deleted → frameshift`
120	`GAGCGCGGCCCGGGTCGCGGGGACGGCGC------------------------TTGAACC`	`24 bp deleted → no frameshift`
120	`GAGCGCGG------------------------GACAGCGCTGCTTACCCGTGCTTGAACC`	`24 bp deleted → no frameshift`
120	`GAGCGCGGCCCG--------------------------------------TGCTTGAACC`	`38 bp deleted → frameshift`
114	`GAGCGCGGCCCGGGTCGCGGGGACG-------------------------TGCTTGAACC`	`25 bp deleted → frameshift`
114	`GAGCGCGGCCCGGGTCGC-------------------------TTACCCGTGCTTGAACC`	`25 bp deleted → frameshift`
114	`GAGCGCGG-------------------------ACAGCGCTGCTTACCCGTGCTTGAACC`	`25 bp deleted → frameshift`
109	`GAGCGCGGCCCGGGTCGCGGGGACGGC--------------------------TTGAACC`	`26 bp deleted → frameshift`
103	`GAGCGCGGCCCG---------------------------CTGCTTACCCGTGCTTGAACC`	`27 bp deleted → no frameshift`
95	`GAGCGCGGCCCGGGTCGCGGGGAC-----------------------CCGTGCTTGAACC`	`23 bp deleted → frameshift`
94	`GAGCGCGGC-----------------------------GCTGCTTACCCGTGCTTGAACC`	`29 bp deleted → frameshift`
84	`GAGCGCGGCCCGGGTCGCG-------------------------------TGCTTGAACC`	`31 bp deleted → frameshift`
84	`GAGCGCGGC-------------------------------TGCTTACCCGTGCTTGAACC`	`31 bp deleted → frameshift`
76	`GAGCGCGGCCCGGGTCG---------------------------------TGCTTGAACC`	`33 bp deleted → no frameshift`
73	`GAGCGCGGC----------------------------------TTACCCGTGCTTGAACC`	`34 bp deleted → frameshift`
69	`GAGCGCGGCCCGGGTCGC-----------------------------------TTGAACC`	`35 bp deleted → frameshift`
66	`GAGC------------------------------------TGCTTACCCGTGCTTGAACC`	`36 bp deleted → no frameshift`
62	`GAGCGCGGCCC-------------------------------------CGTGCTTGAACC`	`37 bp deleted → frameshift`
62	`GAGCGC-------------------------------------TTACCCGTGCTTGAACC`	`37 bp deleted → frameshift`
60	`GA--------------------------------CAGCGCTGCTTACCCGTGCTTGAACC`	`32 bp deleted → frameshift`
56	`GAGCGCGGCC---------------------------------------GTGCTTGAACC`	`39 bp deleted → no frameshift`
56	`GAGC---------------------------------------TTACCCGTGCTTGAACC`	`39 bp deleted → no frameshift`
54	`GAGCGCGGCCCGGGTCGCGGGGA----------------------------------ACC`	`34 bp deleted → frameshift`
52	`GAGCGCGGCCCGGGTCGCGGGGAC-----------------------------------C`	`35 bp deleted → frameshift`
49	`GAGCGCGGCCCGGGT------------------------------------GCTTGAACC`	`36 bp deleted → no frameshift`
46	`GAGCGCG-------------------------------------------TGCTTGAACC`	`43 bp deleted → frameshift`
44	`GAGCGCGGC--------------------------------------------TTGAACC`	`44 bp deleted → frameshift`
42	`GAGCG---------------------------------------------TGCTTGAACC`	`45 bp deleted → no frameshift`
38	`GAGCGC-----------------------------------------------TTGAACC`	`47 bp deleted → frameshift`
34	`GAGCGCGGCCC-------------------------------------------------`	`49 bp deleted → frameshift`
34	`GAGC-------------------------------------------------TTGAACC`	`49 bp deleted → frameshift`
32	`GAGCGCGGCC--------------------------------------------------`	`50 bp deleted → frameshift`
19	`GA-------------------------------------------------------ACC`	`55 bp deleted → frameshift`

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