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Contents:

PCR primers for off-targets of CGGGTCGCGGGGACGGCGCG GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
mm0_exon_Pop5_chr5_115238083_F Primer3: not found at this Tm N.d. 1.00
mm0_exon_Pop5_chr5_115238083_R Primer3: not found at this Tm N.d. 1.00
mm4_intergenic_Prc1|6330403N20Rik_chr7_80294746_F Primer3: not found at this Tm N.d. 0.65
mm4_intergenic_Prc1|6330403N20Rik_chr7_80294746_R Primer3: not found at this Tm N.d. 0.65
mm4_intergenic_Dnaja2|Neto2_chr8_85555324_F Primer3: not found at this Tm N.d. 0.55
mm4_intergenic_Dnaja2|Neto2_chr8_85555324_R Primer3: not found at this Tm N.d. 0.55
mm4_intron_Pafah1b2_chr9_45984532_F TCGTCGGCAGCGTCCTGGCCAGGGTGGAACCAG 62.5 0.39
mm4_intron_Pafah1b2_chr9_45984532_R GTCTCGTGGGCTCGGGTATTGTGAGGCCCGGCTC 60.5 0.39
mm4_exon_2410017I17Rik/Gm17782_chr17_36161918_F TCGTCGGCAGCGTCCGACGTGGCTGGAGAAGG 60.1 0.34
mm4_exon_2410017I17Rik/Gm17782_chr17_36161918_R GTCTCGTGGGCTCGGGAGGGAAAAGGCTTCTGGGG 60.3 0.34
mm4_intron_Psmd11_chr11_80428763_F TCGTCGGCAGCGTCCGACATCCTCCACTCCATCG 59.9 0.29
mm4_intron_Psmd11_chr11_80428763_R GTCTCGTGGGCTCGGTGAGTCAGCAGCACCATAGC 60.1 0.29
mm4_intergenic_Mir133a-2|Slco4a1_chr2_180430464_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Mir133a-2|Slco4a1_chr2_180430464_R Primer3: not found at this Tm N.d. 0.28
mm4_intron_Mllt10_chr2_18065540_F TCGTCGGCAGCGTCAAGTGTCATTAGGTGGCCGC 60.6 0.26
mm4_intron_Mllt10_chr2_18065540_R GTCTCGTGGGCTCGGCCTCCGGTTCCCAAAGCC 60.3 0.26
mm3_intergenic_Atxn7l3|Ubtf_chr11_102296656_F Primer3: not found at this Tm N.d. 0.25
mm3_intergenic_Atxn7l3|Ubtf_chr11_102296656_R Primer3: not found at this Tm N.d. 0.25
mm4_exon_Brinp1_chr4_68954103_F TCGTCGGCAGCGTCTTATTCGGCTCGGTGGGAAC 60.1 0.22
mm4_exon_Brinp1_chr4_68954103_R GTCTCGTGGGCTCGGGTATGAGCCAGGCTGCTAGG 59.9 0.22
mm3_intron_Trim44_chr2_102400901_F Primer3: not found at this Tm N.d. 0.22
mm3_intron_Trim44_chr2_102400901_R Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Vapa|Txndc2_chr17_65613727_F TCGTCGGCAGCGTCCCGCTGTCAATCTCCGTAGA 59.2 0.19
mm4_intergenic_Vapa|Txndc2_chr17_65613727_R GTCTCGTGGGCTCGGCCCCAAGAACGACACCCC 59.9 0.19
mm3_exon_Prkar1b_chr5_139129690_F Primer3: not found at this Tm N.d. 0.19
mm3_exon_Prkar1b_chr5_139129690_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_n-R5s42|Fam19a5_chr15_87625193_F TCGTCGGCAGCGTCGGCCCGAGACTTTCTGCTAA 59.7 0.17
mm4_intergenic_n-R5s42|Fam19a5_chr15_87625193_R GTCTCGTGGGCTCGGGGGAGGCGATCTGTGTCTTT 59.7 0.17
mm4_intergenic_Gm15734|9330182L06Rik_chr5_9266084_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm15734|9330182L06Rik_chr5_9266084_R Primer3: not found at this Tm N.d. 0.17
mm4_exon_Anapc5_chr5_122821208_F TCGTCGGCAGCGTCTGAAGTCCTGGCTCTTCGTG 59.6 0.16
mm4_exon_Anapc5_chr5_122821208_R GTCTCGTGGGCTCGGACAGTCTTGGGCGGGTTAAG 59.9 0.16
mm4_exon_Ift57_chr16_49699392_F TCGTCGGCAGCGTCCTTGGACGATGGGGTGTCTC 60.1 0.15
mm4_exon_Ift57_chr16_49699392_R GTCTCGTGGGCTCGGAACATGTGGTAGGCCGCTC 60.0 0.15
mm4_exon_Raly/a_chr2_154791361_F TCGTCGGCAGCGTCCTCGCGCCTCTGTCAGTG 60.5 0.15
mm4_exon_Raly/a_chr2_154791361_R GTCTCGTGGGCTCGGGAGAGTCGCGACCGCTTC 60.5 0.15
mm3_intergenic_Gm9755|Nat3_chr8_67523121_F TCGTCGGCAGCGTCAGCACGCAGATTCCAGACAA 59.9 0.14
mm3_intergenic_Gm9755|Nat3_chr8_67523121_R GTCTCGTGGGCTCGGCTTTTGAGCTGTTTCCTCCCC 59.1 0.14
mm4_intron_Ephx4_chr5_107403456_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Ephx4_chr5_107403456_R Primer3: not found at this Tm N.d. 0.14
mm4_exon_Mapk15_chr15_75998990_F TCGTCGGCAGCGTCACCCACCCACCTCCTTGAG 60.8 0.14
mm4_exon_Mapk15_chr15_75998990_R GTCTCGTGGGCTCGGGAGGCAGAGGAGGGAGAAGA 60.0 0.14
mm4_intergenic_Gm12036|Aftph_chr11_20741041_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm12036|Aftph_chr11_20741041_R Primer3: not found at this Tm N.d. 0.14
mm4_exon_Zfp365_chr10_67912512_F Primer3: not found at this Tm N.d. 0.14
mm4_exon_Zfp365_chr10_67912512_R Primer3: not found at this Tm N.d. 0.14
mm4_exon_Arpc5_chr1_152766519_F TCGTCGGCAGCGTCCCTCCTTCTCCAGCCCCG 61.4 0.13
mm4_exon_Arpc5_chr1_152766519_R GTCTCGTGGGCTCGGTCTGCTTGCAGCCATCCTTC 60.6 0.13
mm4_intron_Heca_chr10_17947415_F TCGTCGGCAGCGTCGAGCACTTCCTGACTCGCAT 60.1 0.13
mm4_intron_Heca_chr10_17947415_R GTCTCGTGGGCTCGGCGGGAGACGCTAAGAATGGT 59.5 0.13
mm4_exon_Ccm2_chr11_6546972_F Primer3: not found at this Tm N.d. 0.12
mm4_exon_Ccm2_chr11_6546972_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm8935|Gm2464_chr3_13471604_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm8935|Gm2464_chr3_13471604_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Vegfb|Dnajc4_chr19_6987653_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Vegfb|Dnajc4_chr19_6987653_R Primer3: not found at this Tm N.d. 0.11
mm3_exon_Upp2_chr2_58567489_F Primer3: not found at this Tm N.d. 0.11
mm3_exon_Upp2_chr2_58567489_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Pde7a|Gm22282_chr3_19311444_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Pde7a|Gm22282_chr3_19311444_R Primer3: not found at this Tm N.d. 0.11
mm4_exon_2410012M07Rik_chr9_98865018_F Primer3: not found at this Tm N.d. 0.11
mm4_exon_2410012M07Rik_chr9_98865018_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_4732418A04Rik|Scmh1_chr4_120405120_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_4732418A04Rik|Scmh1_chr4_120405120_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Hgsnat_chr8_25976477_F TCGTCGGCAGCGTCCGCGACGCTCAAGTCCTG 61.1 0.10
mm4_intron_Hgsnat_chr8_25976477_R GTCTCGTGGGCTCGGCGGAGCCAGGTGAGCGAG 62.5 0.10
mm2_exon_Reps1_chr10_18055897_F Primer3: not found at this Tm N.d. 0.09
mm2_exon_Reps1_chr10_18055897_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm20204|Cmip_chr8_117256793_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm20204|Cmip_chr8_117256793_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Lrp5|1810055G02Rik_chr19_3686601_F TCGTCGGCAGCGTCGGGAGGAGGGAGCGGAAG 61.1 0.08
mm4_intergenic_Lrp5|1810055G02Rik_chr19_3686601_R GTCTCGTGGGCTCGGCTTGCCCGGCCTCTCCTG 62.4 0.08
mm4_exon_Hipk3_chr2_104494007_F TCGTCGGCAGCGTCTGCCTGGCTTCCTTACCTTC 59.6 0.08
mm4_exon_Hipk3_chr2_104494007_R GTCTCGTGGGCTCGGCCTGCTGAAGCGCTTGGC 62.1 0.08
mm3_intron_Wwp2_chr8_107504744_F Primer3: not found at this Tm N.d. 0.07
mm3_intron_Wwp2_chr8_107504744_R Primer3: not found at this Tm N.d. 0.07
mm4_exon_Dscam_chr16_97170325_F Primer3: not found at this Tm N.d. 0.06
mm4_exon_Dscam_chr16_97170325_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Draxin|Mad2l2_chr4_148130701_F Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Draxin|Mad2l2_chr4_148130701_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Dlgap4/4930405A21Rik|Dlgap4_chr2_156720992_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Dlgap4/4930405A21Rik|Dlgap4_chr2_156720992_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Zfp61_chr7_24299088_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Zfp61_chr7_24299088_R Primer3: not found at this Tm N.d. 0.05
mm4_exon_Rragc/4930535I16Rik_chr4_123917648_F Primer3: not found at this Tm N.d. 0.04
mm4_exon_Rragc/4930535I16Rik_chr4_123917648_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ythdf2_chr4_132211293_F TCGTCGGCAGCGTCCCGGCCGCTCATGAATTACT 60.5 0.04
mm4_intron_Ythdf2_chr4_132211293_R GTCTCGTGGGCTCGGCCCGGGGAAAGGACCTTTC 60.0 0.04
mm3_intergenic_Chd6|Gm14243_chr2_161109343_F TCGTCGGCAGCGTCCTAGATCTCCCCGCCTTGC 59.6 0.03
mm3_intergenic_Chd6|Gm14243_chr2_161109343_R GTCTCGTGGGCTCGGGCTTGCAAAACAGAGCGCAG 61.2 0.03
mm4_intron_Zzz3_chr3_152396157_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Zzz3_chr3_152396157_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm13012|Ece1_chr4_137897855_F TCGTCGGCAGCGTCGCCCTCAGTTTTCCCATCCC 60.6 0.03
mm4_intergenic_Gm13012|Ece1_chr4_137897855_R GTCTCGTGGGCTCGGTGTCCCCATCCACAAATGCA 59.8 0.03
mm3_intron_Sctr_chr1_120007338_F Primer3: not found at this Tm N.d. 0.03
mm3_intron_Sctr_chr1_120007338_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_D030056L22Rik_chr19_18713288_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_D030056L22Rik_chr19_18713288_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Pigg|Gm10419_chr5_108367849_F TCGTCGGCAGCGTCCTCTTCATCCTCCCGCGTC 59.9 0.03
mm4_intergenic_Pigg|Gm10419_chr5_108367849_R GTCTCGTGGGCTCGGCGGCTTGATTCCCGGTGC 61.5 0.03
mm4_exon_Ncor1_chr11_62457193_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_Ncor1_chr11_62457193_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Nup205_chr6_35177698_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Nup205_chr6_35177698_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Cryl1_chr14_57363345_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Cryl1_chr14_57363345_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Fam65a_chr8_105605464_F TCGTCGGCAGCGTCTTTCCTAAGGCCGTGGCG 60.0 0.02
mm4_intron_Fam65a_chr8_105605464_R GTCTCGTGGGCTCGGGAAAAGACACGGCGCGATC 59.9 0.02
mm4_intron_Setd3_chr12_108178828_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Setd3_chr12_108178828_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Nfix_chr8_84799971_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Nfix_chr8_84799971_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Eya1_chr1_14309816_F TCGTCGGCAGCGTCCTCTCAGAGGCGCTGTGAC 60.1 0.02
mm4_exon_Eya1_chr1_14309816_R GTCTCGTGGGCTCGGCTCAGTCGCTCCACAGCC 60.1 0.02
mm4_intron_Arid2_chr15_96288304_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Arid2_chr15_96288304_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Ppp2r3d_chr9_124422717_F TCGTCGGCAGCGTCCCCACATACCTTGGCAACCA 60.2 0.01
mm4_exon_Ppp2r3d_chr9_124422717_R GTCTCGTGGGCTCGGCTACTTCCCTTGCGGTCGAC 60.4 0.01
mm4_intron_Wnt3_chr11_103810234_F TCGTCGGCAGCGTCGAACAAAGGCGGATTGAGCG 60.1 0.01
mm4_intron_Wnt3_chr11_103810234_R GTCTCGTGGGCTCGGCCACGCTCAGTTCCCAGC 60.7 0.01
mm4_intergenic_4933405D12Rik|1810037I17Rik_chr3_122924315_F TCGTCGGCAGCGTCCTAGAAAGGGAGGACGCCTG 59.5 0.01
mm4_intergenic_4933405D12Rik|1810037I17Rik_chr3_122924315_R GTCTCGTGGGCTCGGCGCCCAGTCTAGCAACCAAT 60.3 0.01
mm4_intron_Hecw2_chr1_54194609_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Hecw2_chr1_54194609_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Chpt1_chr10_88503974_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Chpt1_chr10_88503974_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Unc5d|4933433F19Rik_chr8_29219546_F TCGTCGGCAGCGTCGCAGCGGAACAGGGATATCA 59.8 0.01
mm4_intergenic_Unc5d|4933433F19Rik_chr8_29219546_R GTCTCGTGGGCTCGGCAGGCTCGGTTTGGTAGTCC 60.3 0.01
mm4_exon_Rap2a_chr14_120478645_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Rap2a_chr14_120478645_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Prpf40a_chr2_53191094_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Prpf40a_chr2_53191094_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Atg5|Prdm1_chr10_44426219_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Atg5|Prdm1_chr10_44426219_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Hpn|Scn1b_chr7_31115306_F TCGTCGGCAGCGTCCTCACCTGAGGAGGAGGGG 60.3 0.01
mm4_intergenic_Hpn|Scn1b_chr7_31115306_R GTCTCGTGGGCTCGGGTAACTGAGCCTGCCTGGTC 60.3 0.01
mm4_exon_Cnp_chr11_100574910_F TCGTCGGCAGCGTCTTTCTGCCAAGGCTCAGACC 60.2 0.01
mm4_exon_Cnp_chr11_100574910_R GTCTCGTGGGCTCGGGTCCCTTTCATAGCCTGGGG 59.8 0.01
mm4_exon_Zer1_chr2_30124394_F TCGTCGGCAGCGTCAGGAGGTCGGATCCGGTC 60.1 0.01
mm4_exon_Zer1_chr2_30124394_R GTCTCGTGGGCTCGGCCGAGGGCAAGGGAACGG 62.7 0.01
mm4_exon_Necab2_chr8_119446957_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Necab2_chr8_119446957_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Nfe2l1|Copz2_chr11_96849842_F TCGTCGGCAGCGTCGAGAGAGGACGCACACAGAG 59.8 0.01
mm4_intergenic_Nfe2l1|Copz2_chr11_96849842_R GTCTCGTGGGCTCGGCTCGCTGCCTGCACTGAC 61.1 0.01
mm4_intergenic_Gm26885|Pim1/Gm26885_chr17_29490700_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26885|Pim1/Gm26885_chr17_29490700_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26652|Gm22783_chrX_19237887_F TCGTCGGCAGCGTCTTCTGTTACTGGGTCCGCAC 59.9 0.01
mm4_intergenic_Gm26652|Gm22783_chrX_19237887_R GTCTCGTGGGCTCGGTAGGCAGCTCAGAGCCAGT 60.3 0.01
mm4_exon_Sept9_chr11_117266365_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Sept9_chr11_117266365_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cacna1a_chr8_84415305_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Cacna1a_chr8_84415305_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ccdc105|Slc1a6_chr10_78780434_F TCGTCGGCAGCGTCCGTCCCGGAGCTCCTGAG 61.5 0.00
mm4_intergenic_Ccdc105|Slc1a6_chr10_78780434_R GTCTCGTGGGCTCGGAATCGGAGGAATGCAGGCTG 60.4 0.00
mm4_exon_Ube2q2_chr9_55149499_F TCGTCGGCAGCGTCCATTTTCCAGCCGCGCTC 59.8 0.00
mm4_exon_Ube2q2_chr9_55149499_R GTCTCGTGGGCTCGGGGAGGGGTGAGGAAAGGGA 60.2 0.00
mm4_exon_Nt5dc3_chr10_86779128_F TCGTCGGCAGCGTCCACTGGCAGCTTAGAGGCAG 60.7 0.00
mm4_exon_Nt5dc3_chr10_86779128_R GTCTCGTGGGCTCGGGAGCGCGGTCGTTCTCGA 62.4 0.00
mm4_exon_Cask_chrX_13846260_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Cask_chrX_13846260_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Akap9_chr5_3928557_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Akap9_chr5_3928557_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Nr1d1|Msl1_chr11_98795433_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Nr1d1|Msl1_chr11_98795433_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Brd4_chr17_32250539_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Brd4_chr17_32250539_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Foxg1/3110039M20Rik_chr12_49383779_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Foxg1/3110039M20Rik_chr12_49383779_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Rfxank/Nr2c2ap_chr8_70131299_F TCGTCGGCAGCGTCCGAAGGGGAGGTGCCATAAA 59.7 0.00
mm4_exon_Rfxank/Nr2c2ap_chr8_70131299_R GTCTCGTGGGCTCGGTTCTAAACCAACCGAGCCCC 59.9 0.00
mm4_intron_Rab31_chr17_65729983_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rab31_chr17_65729983_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Zfp532_chr18_65581233_F TCGTCGGCAGCGTCCATTTGGGCCCCTCGTGC 61.4 0.00
mm4_intron_Zfp532_chr18_65581233_R GTCTCGTGGGCTCGGCAAAGCCAGGGTGGGACAG 60.6 0.00
mm4_intron_Vkorc1l1_chr5_129942406_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Vkorc1l1_chr5_129942406_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ttll11_chr2_35885012_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ttll11_chr2_35885012_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Thumpd2_chr17_81036170_F TCGTCGGCAGCGTCGTGGCAGAAGTCTTGGGAGG 60.3 0.00
mm4_intron_Thumpd2_chr17_81036170_R GTCTCGTGGGCTCGGCTGTTTGCTTTCTCCACGGC 60.0 0.00
mm4_intron_Tcn2_chr11_3931845_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Tcn2_chr11_3931845_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sh3bp4_chr1_89070773_F TCGTCGGCAGCGTCTTCCCTGCCCTCTGTGGT 60.1 0.00
mm4_intron_Sh3bp4_chr1_89070773_R GTCTCGTGGGCTCGGCGCTCTCTTCGCTCCTTCTC 60.2 0.00
mm4_intron_Ocln_chr13_100552302_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ocln_chr13_100552302_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Klc2_chr19_5118041_F TCGTCGGCAGCGTCAGAAAAGGCCGGATTCCCAG 60.0 0.00
mm4_intron_Klc2_chr19_5118041_R GTCTCGTGGGCTCGGTAGAGGAGGAGTCGTCGTGG 60.1 0.00
mm4_intron_Hivep3_chr4_120032760_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Hivep3_chr4_120032760_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm13589_chr2_38424493_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm13589_chr2_38424493_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Clic4_chr4_135272437_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Clic4_chr4_135272437_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Chd5_chr4_152339057_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Chd5_chr4_152339057_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Adam18_chr8_24653182_F TCGTCGGCAGCGTCTATGGGGTGTGACACAAGGC 59.9 0.00
mm4_intron_Adam18_chr8_24653182_R GTCTCGTGGGCTCGGCTCTGTGGGTTGATGCACCT 59.9 0.00
mm4_intergenic_Pdgfc|Gm22531_chr3_81627977_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pdgfc|Gm22531_chr3_81627977_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Hes1|n-R5s32_chr16_30070396_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Hes1|n-R5s32_chr16_30070396_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm5229|Crim1_chr17_78200144_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm5229|Crim1_chr17_78200144_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26691|Cxxc4_chr3_134202870_F TCGTCGGCAGCGTCTGCTTGGTCTGTCAGCAGTT 59.8 0.00
mm4_intergenic_Gm26691|Cxxc4_chr3_134202870_R GTCTCGTGGGCTCGGTGCAGAGGAGTAAGGGGAGG 60.3 0.00
mm4_intergenic_Gm24269|Tdgf1_chr9_110932021_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24269|Tdgf1_chr9_110932021_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23822|4921504A21Rik_chr5_19024882_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23822|4921504A21Rik_chr5_19024882_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm15563|Usp3_chr9_66592613_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm15563|Usp3_chr9_66592613_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Coq3|Faxc_chr4_21931233_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Coq3|Faxc_chr4_21931233_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ccny|Gm22765_chr18_9450165_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ccny|Gm22765_chr18_9450165_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_BC052040|Meis2_chr2_115859178_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_BC052040|Meis2_chr2_115859178_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_9930021J03Rik|Ranbp6_chr19_29806213_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_9930021J03Rik|Ranbp6_chr19_29806213_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_4930595D18Rik|Traf3_chr12_111166132_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_4930595D18Rik|Traf3_chr12_111166132_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Zbtb9/Ggnbp1_chr17_26973258_F TCGTCGGCAGCGTCGGTTGAGGTGCTCCGACG 60.4 0.00
mm4_exon_Zbtb9/Ggnbp1_chr17_26973258_R GTCTCGTGGGCTCGGCTCCTCACCGTGCTCGCTC 62.6 0.00
mm4_exon_Zbtb14_chr17_69383927_F TCGTCGGCAGCGTCGCGGGCCAAGGAAGAGAG 60.1 0.00
mm4_exon_Zbtb14_chr17_69383927_R GTCTCGTGGGCTCGGCACCTTCCCTTCCACCATCC 60.0 0.00
mm4_exon_Smad6_chr9_64020814_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Smad6_chr9_64020814_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Mpped2_chr2_106693596_F TCGTCGGCAGCGTCTCTCTTCCCAGATCGAGGCT 59.7 0.00
mm4_exon_Mpped2_chr2_106693596_R GTCTCGTGGGCTCGGAGAACCTTCGACCCCGGAG 60.9 0.00
mm4_exon_Map4k3_chr17_80728041_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Map4k3_chr17_80728041_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Grin2c_chr11_115267155_F TCGTCGGCAGCGTCGACCAGTACGGGTAGCGC 59.8 0.00
mm4_exon_Grin2c_chr11_115267155_R GTCTCGTGGGCTCGGCATCTCAGTCGGCTGGTGAG 60.1 0.00
mm4_exon_Gm10657/Cd276_chr9_58555038_F TCGTCGGCAGCGTCACAAAGGTGGACCCAGCAG 59.8 0.00
mm4_exon_Gm10657/Cd276_chr9_58555038_R GTCTCGTGGGCTCGGCAACAGTGGCGGCGATTG 59.8 0.00
mm4_exon_Astn2_chr4_66404541_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Astn2_chr4_66404541_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_4833418N17Rik/Bnc1_chr7_81992395_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_4833418N17Rik/Bnc1_chr7_81992395_R Primer3: not found at this Tm N.d. 0.00
mm2_intron_Mdm1_chr10_118141983_F Primer3: not found at this Tm N.d. 0.00
mm2_intron_Mdm1_chr10_118141983_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm4_intron_Pafah1b2_chr9_45984532 CTGGCCAGGGTGGAACCAGGGCGCCGCAGGGCAGCGGCGACGGCGCGGGGCCCCGGAGGCCCTGCAGGGCGACANNNNNN
NNNNNNNNNNNNNNNNNNNNNNNTGGGGGAGGGGAGCCGGGCCTCACAATAC
mm4_exon_2410017I17Rik/Gm17782_chr17_36161918 CGACGTGGCTGGAGAAGGGGCGGAGGGTCGGGTGGGCGGCGCGGGGACCGGGCTTCCCCTGTGCAGTCCCCGACCCGCCC
ATGCCCCCAGAAGCCTTTTCCCTC
mm4_intron_Psmd11_chr11_80428763 CGACATCCTCCACTCCATCGGTAAAGGGCCTGGCGCCGTCCCCGCGGCTCCGCCGTCCCGGCGCGGCCTGTGTGGGTCGG
AGGCGGCGGAGAGGGGCCGGGCTAGCCGGCTATGGTGCTGCTGACTCA
mm4_intron_Mllt10_chr2_18065540 AAGTGTCATTAGGTGGCCGCCGGCGTCGGGCCGCGTCCTGCGCAGGGTAGGGTGGGAGCGCGAGACCCGCGCTGGAGTCA
CGGTGACGGCGCGTGG
TGGCTTTGGGAACCGGAGG
mm4_exon_Brinp1_chr4_68954103 TTATTCGGCTCGGTGGGAACTTGGAAGAGCCCTGCGTCCAGCTCGCATACCGGGGACGGCGCGGGGACTGCAGGCGTGGG
GGTACCTGGCTCCTAGCAGCCTGGCTCATAC
mm4_intergenic_Vapa|Txndc2_chr17_65613727 CCGCTGTCAATCTCCGTAGAGCAGGGTCGGGGGGGCGGAGCGGGGCTCTTTGCGGGGAGACGCGGTGCACGGCGCCCCGG
GAAGGGGGTGTCGTTCTTGGGG
mm4_intergenic_n-R5s42|Fam19a5_chr15_87625193 GGCCCGAGACTTTCTGCTAACCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTG
CCCAAAAGACACAGATCGCCTCCC
mm4_exon_Anapc5_chr5_122821208 TGAAGTCCTGGCTCTTCGTGGACGCCGGCAAGGACAGGCCCTCTCCAGGCCTGCCACGCGCCGCGCCCACGAGCCGCCTT
TACGCCATGGCTGCAGCCGCCCGGCACCTTAACCCGCCCAAGACTGT
mm4_exon_Ift57_chr16_49699392 CTTGGACGATGGGGTGTCTCGGGCTCGCGGGGAAGGCGCAGGGGAGGCTGTGGTGGAGCGCGGGCCAGGAGCGGCCTACC
ACATGTT
mm4_exon_Raly/a_chr2_154791361 CTCGCGCCTCTGTCAGTGCGGCGGGGCGCGCGAGCGGCGCGGGGTGCGGAGCCGAGGGAAGCCGAGGGGGCGGAAGCGGT
CGCGACTCTC
mm3_intergenic_Gm9755|Nat3_chr8_67523121 AGCACGCAGATTCCAGACAACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNN
NNNNNNNGTGGGGGAGGAAACAGCTCAAAAG
mm4_exon_Mapk15_chr15_75998990 ACCCACCCACCTCCTTGAGGCAACCCGGCGCCGTCCCCCAGACCCCAATGCATCTTCTCCCTCCTCTGCCTC
mm4_exon_Arpc5_chr1_152766519 CCTCCTTCTCCAGCCCCGCCCCTTTCCTGCAGCGGGCCCGCGCGTGCGCGGTGCGTCCCGCGCGCTCCCCTGCCGACGCG
CGCTCCCGGGGGACGCGGGGGCGGAGCGGGGGCTGAAGGATGGCTGCAAGCAGA
mm4_intron_Heca_chr10_17947415 GAGCACTTCCTGACTCGCATTGTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGGTG
AGGAAGTTGGCGCTCGCCCCGCCGCTGTCGTCTTACCATTCTTAGCGTCTCCCG
mm4_intron_Hgsnat_chr8_25976477 CGCGACGCTCAAGTCCTGCGCCCCGCGGGCCGTGGGGAGGTCCGGGGCAGGCCCACGCACGGTCCCCGCTACCCCTAGCC
GTGGGGGCCGCTCGCTCACCTGGCTCCG
mm4_intergenic_Lrp5|1810055G02Rik_chr19_3686601 GGGAGGAGGGAGCGGAAGCCGAGCCGGTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNAGGCGGGTCTCTCCGGGCCCGCGACAGGAGAGGCCGGGCAAG
mm4_exon_Hipk3_chr2_104494007 TGCCTGGCTTCCTTACCTTCCTAGAGGCCCGCGCTCTCCCCGCTTCGGGCGGCCGCAGCCCTGAGCATGTCCGGGGTGGG
TCGCGGGGGCCGTGCGGGG
ACCCGGAGCCAAGCGCTTCAGCAGG
mm4_intron_Ythdf2_chr4_132211293 CCGGCCGCTCATGAATTACTCGTTGTTAACGAAAAAAGCTGCCCGCCAGCTGCAGGGCCACACCAAAAGGGGCGACCGCC
CCGCGCGCGGCACCTGCGACCCGAGCGGCTTCGGAAAGGTCCTTTCCCCGGG
mm3_intergenic_Chd6|Gm14243_chr2_161109343 CTAGATCTCCCCGCCTTGCAGACAGCGCAGCTCCCGGCCCGCCCCCCCGGAATGAAGCGGCCGGGCCGCGNNNNNNNNNN
NNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCGCTCTGTTTTGCAAGC
mm4_intergenic_Gm13012|Ece1_chr4_137897855 GCCCTCAGTTTTCCCATCCCAATCTCAAACATCTTTTCTCTTGTTATCGCTAATTCCAGAGAAGGTTGTCCCCCCGCCAT
CCCCCCGCCCCG
TGACAATAACGGGTGCTTTTGCATTTGTGGATGGGGACA
mm4_intergenic_Pigg|Gm10419_chr5_108367849 CTCTTCATCCTCCCGCGTCCTCCCGGGCTCGCGCCGCTGTCACTGGAGCCCGCATCCTCCGCCTCCCGCCGCCGCCGCGA
CCCC
GCACCGGGAATCAAGCCG
mm4_intron_Fam65a_chr8_105605464 TTTCCTAAGGCCGTGGCGGGGAGAAGGGGCGAGCTGTGGATCCCGTGCGTCCTGCACCCGCGCCGCCCATCGGCTCCCGG
ACTTGCGACCGGGTCCCGCCTCACCCCGTTCTCGCGACCCGGATCGCGCCGTGTCTTTTC
mm4_exon_Eya1_chr1_14309816 CTCTCAGAGGCGCTGTGACGCGGCTGGCAGCGGAGCGCAGAGGCCGGGGATTGCCCGGCTCCCGGGGACTGCGCCCGGGC
GCTGCTGCAGGCTCGGGCTGTGGAGCGACTGAG
mm4_exon_Ppp2r3d_chr9_124422717 CCCACATACCTTGGCAACCACGGCCATGTCGCCCAGACCCGCCCTCCCACGAGGCAGCCCCTCGAACGCGCACTCCACGA
GGGCGATGGCATCCTCCGTGTCCTGGGGACGGGGCGGGGGTCGACCGCAAGGGAAGTAG
mm4_intron_Wnt3_chr11_103810234 GAACAAAGGCGGATTGAGCGGCGCAGCGGGTCCCGCCGGACGGGGACCGGCACAGCGGGGCTGGCAGCGCCGCTCCCAGG
CTAATCCCCGCCCCGCCCCCGCCACCCCCACTGCCCCGGCGCTGGGAACTGAGCGTGG
mm4_intergenic_4933405D12Rik|1810037I17Rik_chr3_122924315 CTAGAAAGGGAGGACGCCTGCGCAGTCCGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGC
CTAGTCGATTGGCGGGGTTACCCGGAATTGGTTGCTAGACTGGGCG
mm4_intergenic_Unc5d|4933433F19Rik_chr8_29219546 GCAGCGGAACAGGGATATCAGATGGGCAGCGGGACGCAGCGCGCGCCCCCATCGCCTCCTGCGGCGCCGCCCGCCGTCCC
CGCTAAGCG
GCCCGCAGCCCCAGCTCCGCTCCGGGTCCGGGACTACCAAACCGAGCCTG
mm4_intergenic_Hpn|Scn1b_chr7_31115306 CTCACCTGAGGAGGAGGGGGCTGGGTGGGAGGCGGGGACGGGGCGGGGAAGGGCGGACCCCAGGACAGGCTGCCACAGAG
GCCACCTGGCTCATGGGGCGGGGCCCGCAGGGGTTGGACCAGGCAGGCTCAGTTAC
mm4_exon_Cnp_chr11_100574910 TTTCTGCCAAGGCTCAGACCGCTGCTTCCTGGCACTCAGGTTTCCATTCCCCGGGGGCGCGTGCGCAGAGGCTCGGGCTG
ACCCCGCCCTGTCCGCGCGCCCCGCCCCCAGGCTATGAAAGGGAC
mm4_exon_Zer1_chr2_30124394 AGGAGGTCGGATCCGGTCCGGGTCCGGGTCNNNNNNNNNNNNNNNNNNNNNNNNNNAATGCTGCGACCGGAGCCCCGGAC
GGAGGCCGTTCCCTTGCCCTCGG
mm4_intergenic_Nfe2l1|Copz2_chr11_96849842 GAGAGAGGACGCACACAGAGGCCAGCCACGCAGGACTGGGCGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNTGTCAGTGCAGGCAGCGAG
mm4_intergenic_Gm26652|Gm22783_chrX_19237887 TTCTGTTACTGGGTCCGCACTCGCCCAACCCGAGCCTTCATCCCCGCCCCTTTCCCGCCACCCGCGCAGCACTGTTCTGC
GCTNNNNNNNNNNNNNNNNNNNNNNNNATCGCTGCGGGGACTGGCTCTGAGCTGCCTA
mm4_intergenic_Ccdc105|Slc1a6_chr10_78780434 CGTCCCGGAGCTCCTGAGCAGCTGCGGCAGGGGGACATCCCCGAGGGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNACCCACGTGAGCTCAAGGCCAGCCTGCATTCCTCCGATT
mm4_exon_Ube2q2_chr9_55149499 CATTTTCCAGCCGCGCTCGGACGCGGGTCGCGGCGGCGGGGAGAGGCGGAGCCGCGAGANNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCCTTTCCTCACCCCTCC
mm4_exon_Nt5dc3_chr10_86779128 CACTGGCAGCTTAGAGGCAGCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNTCGAGAACGACCGCGCTC
mm4_exon_Rfxank/Nr2c2ap_chr8_70131299 CGAAGGGGAGGTGCCATAAACCCCGCCCCGGCCCCGCGAACGGAACAGTGTCTGTGTAGCACTGCGCGTGCGCTATCCGC
ACGCGGTTCCGGGTTCGTAGATCTGCAGAGCCGCTGGGGCTCGGTTGGTTTAGAA
mm4_intron_Zfp532_chr18_65581233 CATTTGGGCCCCTCGTGCCACCCCGCGGGTCACGGCGAGCGCGCGAGGCTCAGGCCCCTTGCCTTGGCCTGAGTGCGGCG
GCCTGTCCCACCCTGGCTTTG
mm4_intron_Thumpd2_chr17_81036170 GTGGCAGAAGTCTTGGGAGGGGTGCGGGGCGCGGGGTGGGGGCGAGGAGTCTGTCTGCCCTCCCTCAGGTATGCAGGGTG
CCGTGGAGAAAGCAAACAG
mm4_intron_Sh3bp4_chr1_89070773 TTCCCTGCCCTCTGTGGTCACAGCCCGACCTGGGCTGCGGATCGTGGTCGCTANNNNNNNNNNNNNNNNNNNNNNNNNNN
N
NNNNNNNNNNNNNNNNNNNNNNTTCTGAGAAGGAGCGAAGAGAGCG
mm4_intron_Klc2_chr19_5118041 AGAAAAGGCCGGATTCCCAGCCCCAGCCCGGGACCGCGCCCCCTCCCCGCGGGCCGGTCGGGTCCTCCCACAGGCCGCGC
CCACGACCACGACGACTCCTCCTCTA
mm4_intron_Adam18_chr8_24653182 TATGGGGTGTGACACAAGGCCCTGCCTCAGGCTGAGATCCTCGCCCCCTCCCCCCGCCCCGACACCCACCTTCCTAGGAT
GATACTCACTTCCCTTCCAGGTGCATCAACCCACAGAG
mm4_intergenic_Gm26691|Cxxc4_chr3_134202870 TGCTTGGTCTGTCAGCAGTTGGAAAGGGAGACCAGGTTTTTAAAGTGATTCCTCCCTCCCTGCCCCCCCCCCTCCCCGCA
ACCCG
ATGGTGTGCAGTTCCTTCAAACAACCCTCCCCTTACTCCTCTGCA
mm4_exon_Zbtb9/Ggnbp1_chr17_26973258 GGTTGAGGTGCTCCGACGCGGTCGCGCGTGCGGTGGGGGCGTGTCGGTGGGACGTCGCGGGGCCGGAGCTCACTGAGCCG
GCCGGAGCGAGCACGGTGAGGAG
mm4_exon_Zbtb14_chr17_69383927 GCGGGCCAAGGAAGAGAGCCTCGCGCCCGCGCCGCGACCTGGAGAAGTGGAGGATGGTGGAAGGGAAGGTG
mm4_exon_Mpped2_chr2_106693596 TCTCTTCCCAGATCGAGGCTGCTCCGGTCCTCTCCACCTCCCCACGCAGCGACCCCGCGTCCCGCTCGCACCGCGCCCCT
GTGCTGCCCTCCCAGGCCCTGGATCGGCGGCGGGGACCTCCGGGGTCGAAGGTTCT
mm4_exon_Grin2c_chr11_115267155 GACCAGTACGGGTAGCGCGGCCTGAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTCCCGGGC
TGCGCAAAGGCGCGCCTCCCGCTCTCGGGGCTCACCAGCCGACTGAGATG
mm4_exon_Gm10657/Cd276_chr9_58555038 ACAAAGGTGGACCCAGCAGCCCACGCCGCCCCGCGCCACGCAAGCACTCACCGCGCCGCGCCGACTCCCGGGACTCGGGG
ACGCGGCGGGG
CGCAGCCCAATCGCCGCCACTGTTG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_ozJbN0W08qlgEBn3RvZK.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.