CRISPOR

DNA break repair outcome predictions

Guide: CCATGGTGCGGTTCAAGCAC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TCCCGCATCCGGCGCGGACGCCATGGTGCGGTTCAAGCACGGGTAAGCAGCGCTGTCCCCGCGCCGTCCCCGCGACCCGG`	(Wild-type sequence)
424	`GGCGCGGACGCCATGGTGCGGTTCAAGCA----------GCGCTGTCCCCGCGCCGTCCC`	`10 bp deleted → frameshift`
274	`GGCGCGGACGCCATGGTGCGGT------------AAGCAGCGCTGTCCCCGCGCCGTCCC`	`12 bp deleted → no frameshift`
261	`GGCGCGGACGCCATGGTGCGGTTCAAGC-------------GCTGTCCCCGCGCCGTCCC`	`13 bp deleted → frameshift`
230	`GGCGCGGACGCCATGGTGCGG-----------GTAAGCAGCGCTGTCCCCGCGCCGTCCC`	`11 bp deleted → frameshift`
213	`GGCGCGGACGCCATGGT-----------------AAGCAGCGCTGTCCCCGCGCCGTCCC`	`17 bp deleted → frameshift`
199	`GGCGCGGACGCCATGGTGCG----------------------CTGTCCCCGCGCCGTCCC`	`22 bp deleted → frameshift`
188	`GGCGCGGACGCCATGGTGCGGTTCAAGC---------------TGTCCCCGCGCCGTCCC`	`15 bp deleted → no frameshift`
179	`GGCGCGGACGCCATGG----------------GTAAGCAGCGCTGTCCCCGCGCCGTCCC`	`16 bp deleted → frameshift`
154	`GGCGCGGACGCCATGGTGC-------------------AGCGCTGTCCCCGCGCCGTCCC`	`19 bp deleted → frameshift`
149	`GGCGCGGACGCCATGGTGCGGTTCA--------------GCGCTGTCCCCGCGCCGTCCC`	`14 bp deleted → frameshift`
121	`GGCGCGGACGC--------------------------------TGTCCCCGCGCCGTCCC`	`32 bp deleted → frameshift`
120	`GGCGCGGACGCCATGGTGCGGTTCAAGC------------------------GCCGTCCC`	`24 bp deleted → no frameshift`
120	`GGCGCGGACGCCATGGTGC------------------------TGTCCCCGCGCCGTCCC`	`24 bp deleted → no frameshift`
120	`GGCGC--------------------------------------TGTCCCCGCGCCGTCCC`	`38 bp deleted → frameshift`
115	`GGCGCGGACGCCATGGTGCG---------------------------------CCGTCCC`	`33 bp deleted → no frameshift`
114	`GGCGCGG-------------------------GTAAGCAGCGCTGTCCCCGCGCCGTCCC`	`25 bp deleted → frameshift`
109	`GGCGCGGACGCCATGGTGCGGTTCAAGC--------------------------CGTCCC`	`26 bp deleted → frameshift`
109	`GGCGCGG--------------------------TAAGCAGCGCTGTCCCCGCGCCGTCCC`	`26 bp deleted → frameshift`
103	`GGCGCGGACGC---------------------------AGCGCTGTCCCCGCGCCGTCCC`	`27 bp deleted → no frameshift`
99	`GGCGCG------------------------------------CTGTCCCCGCGCCGTCCC`	`36 bp deleted → no frameshift`
95	`GGCGCGGACGCCATGGTGCGGTTC-----------------------CCCGCGCCGTCCC`	`23 bp deleted → frameshift`
92	`GGCGCGGACGCC-------------------------------------------GTCCC`	`43 bp deleted → frameshift`
90	`GGCGCGGACGCCATGGTGCGGT------------------------CCCCGCGCCGTCCC`	`24 bp deleted → no frameshift`
89	`GGCGCGGACGC------------------------------GCTGTCCCCGCGCCGTCCC`	`30 bp deleted → no frameshift`
89	`GG------------------------------GTAAGCAGCGCTGTCCCCGCGCCGTCCC`	`30 bp deleted → no frameshift`
84	`GGCGCGGACGCCATGGTGCG-------------------------------CGCCGTCCC`	`31 bp deleted → frameshift`
84	`GG-------------------------------TAAGCAGCGCTGTCCCCGCGCCGTCCC`	`31 bp deleted → frameshift`
81	`GGCGCGGACGCCA--------------------------GCGCTGTCCCCGCGCCGTCCC`	`26 bp deleted → frameshift`
77	`GGCGCGGACGCCATGGTG---------------------------TCCCCGCGCCGTCCC`	`27 bp deleted → no frameshift`
77	`GGCGCGGACGC-----------------------------------------GCCGTCCC`	`41 bp deleted → frameshift`
76	`GGCGC---------------------------------AGCGCTGTCCCCGCGCCGTCCC`	`33 bp deleted → no frameshift`
76	`GGCGCG-----------------------------------------------CCGTCCC`	`47 bp deleted → frameshift`
70	`GGCGCGGACGCCATGGT-----------------------------CCCCGCGCCGTCCC`	`29 bp deleted → frameshift`
69	`GGCGCGGACGCCATGGTGC-----------------------------------CGTCCC`	`35 bp deleted → frameshift`
69	`GGC-----------------------------------AGCGCTGTCCCCGCGCCGTCCC`	`35 bp deleted → frameshift`
68	`GGCGC-------------------------------------------------CGTCCC`	`49 bp deleted → frameshift`
66	`GGCGCGGACGCCATG------------------------------TCCCCGCGCCGTCCC`	`30 bp deleted → no frameshift`
66	`GGCGCGGACGCCATGGTGCG------------------------------------TCCC`	`36 bp deleted → no frameshift`
66	`GGCGCGGACGCC------------------------------------CCGCGCCGTCCC`	`36 bp deleted → no frameshift`
62	`GGCGCGGACGCC-------------------------------------CGCGCCGTCCC`	`37 bp deleted → frameshift`
60	`GGCGCGGACGCC--------------------------------------GCGCCGTCCC`	`38 bp deleted → frameshift`
54	`GGCGCGGACGCCATGGTGCGGTTC----------------------------------CC`	`34 bp deleted → frameshift`
54	`GGC----------------------------------------TGTCCCCGCGCCGTCCC`	`40 bp deleted → frameshift`
52	`GGCGCGGACGCCATGGTGCGGT-----------------------------------CCC`	`35 bp deleted → frameshift`
42	`GGCGCG---------------------------------------------CGCCGTCCC`	`45 bp deleted → no frameshift`
40	`GGCGCGGACGCCATGGT----------------------------------------CCC`	`40 bp deleted → frameshift`
40	`GGCGCGGACG----------------------------------------------TCCC`	`46 bp deleted → frameshift`
38	`GGCGCGGACGCC-----------------------------------------------C`	`47 bp deleted → frameshift`
36	`GGCGCGGACGCC------------------------------------------------`	`48 bp deleted → no frameshift`
32	`GGCGCG--------------------------------------------------TCCC`	`50 bp deleted → frameshift`
31	`GGC---------------------------------------------------CGTCCC`	`51 bp deleted → no frameshift`
29	`GGCG----------------------------------------------------TCCC`	`52 bp deleted → frameshift`

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