CRISPOR

DNA break repair outcome predictions

Guide: GCCATGGTGCGGTTCAAGCA CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTCCCGCATCCGGCGCGGACGCCATGGTGCGGTTCAAGCACGGGTAAGCAGCGCTGTCCCCGCGCCGTCCCCGCGACCCG`	(Wild-type sequence)
424	`CGGCGCGGACGCCATGGTGCGGTTCAAGCA----------GCGCTGTCCCCGCGCCGTCC`	`10 bp deleted → frameshift`
346	`CGGCGCGGACGCCATGGTGCGG-----------GTAAGCAGCGCTGTCCCCGCGCCGTCC`	`11 bp deleted → frameshift`
274	`CGGCGCGGACGCCATGGTGCGGT------------AAGCAGCGCTGTCCCCGCGCCGTCC`	`12 bp deleted → no frameshift`
261	`CGGCGCGGACGCCATGGTGCGGTTCAAGC-------------GCTGTCCCCGCGCCGTCC`	`13 bp deleted → frameshift`
213	`CGGCGCGGACGCCATGGT-----------------AAGCAGCGCTGTCCCCGCGCCGTCC`	`17 bp deleted → frameshift`
199	`CGGCGCGGACGCCATGGTGCG----------------------CTGTCCCCGCGCCGTCC`	`22 bp deleted → frameshift`
188	`CGGCGCGGACGCCATGGTGCGGTTCAAGC---------------TGTCCCCGCGCCGTCC`	`15 bp deleted → no frameshift`
179	`CGGCGCGGACGCCATGG----------------GTAAGCAGCGCTGTCCCCGCGCCGTCC`	`16 bp deleted → frameshift`
172	`CGGCGCGG-------------------------GTAAGCAGCGCTGTCCCCGCGCCGTCC`	`25 bp deleted → frameshift`
154	`CGGCGCGGACGCCATGGTGC-------------------AGCGCTGTCCCCGCGCCGTCC`	`19 bp deleted → frameshift`
149	`CGGCGCGGACGCCATGGTGCGGTTCA--------------GCGCTGTCCCCGCGCCGTCC`	`14 bp deleted → frameshift`
140	`CGGCGCGGACG---------------------GGTAAGCAGCGCTGTCCCCGCGCCGTCC`	`21 bp deleted → no frameshift`
133	`CGG------------------------------GTAAGCAGCGCTGTCCCCGCGCCGTCC`	`30 bp deleted → no frameshift`
121	`CGGCGCGGACGC--------------------------------TGTCCCCGCGCCGTCC`	`32 bp deleted → frameshift`
120	`CGGCGCGGACGCCATGGTGCGGTTCAAGC------------------------GCCGTCC`	`24 bp deleted → no frameshift`
120	`CGGCGCGGACGCCATGGTGC------------------------TGTCCCCGCGCCGTCC`	`24 bp deleted → no frameshift`
120	`CGGCGC--------------------------------------TGTCCCCGCGCCGTCC`	`38 bp deleted → frameshift`
115	`CGGCGCGGACGCCATGGTGCG---------------------------------CCGTCC`	`33 bp deleted → no frameshift`
109	`CGGCGCGGACGCCATGGTGCGGTTCAAGC--------------------------CGTCC`	`26 bp deleted → frameshift`
109	`CGGCGCGG--------------------------TAAGCAGCGCTGTCCCCGCGCCGTCC`	`26 bp deleted → frameshift`
103	`CGGCGCGGACGC---------------------------AGCGCTGTCCCCGCGCCGTCC`	`27 bp deleted → no frameshift`
103	`CGGCG---------------------------GGTAAGCAGCGCTGTCCCCGCGCCGTCC`	`27 bp deleted → no frameshift`
99	`CGGCGCG------------------------------------CTGTCCCCGCGCCGTCC`	`36 bp deleted → no frameshift`
95	`CGGCGCGGACGCCATGGTGCGGTTC-----------------------CCCGCGCCGTCC`	`23 bp deleted → frameshift`
92	`CGGCGCGGACGCC-------------------------------------------GTCC`	`43 bp deleted → frameshift`
90	`CGGCGCGGACGCCATGGTGCGGT------------------------CCCCGCGCCGTCC`	`24 bp deleted → no frameshift`
89	`CGGCGCGGACGC------------------------------GCTGTCCCCGCGCCGTCC`	`30 bp deleted → no frameshift`
84	`CGGCGCGGACGCCATGGTGCG-------------------------------CGCCGTCC`	`31 bp deleted → frameshift`
84	`CGG-------------------------------TAAGCAGCGCTGTCCCCGCGCCGTCC`	`31 bp deleted → frameshift`
81	`CGGCGCGGACGCCA--------------------------GCGCTGTCCCCGCGCCGTCC`	`26 bp deleted → frameshift`
77	`CGGCGCGGACGCCATGGTG---------------------------TCCCCGCGCCGTCC`	`27 bp deleted → no frameshift`
77	`CGGCGCGGACGC-----------------------------------------GCCGTCC`	`41 bp deleted → frameshift`
76	`CGGCGC---------------------------------AGCGCTGTCCCCGCGCCGTCC`	`33 bp deleted → no frameshift`
76	`CGGCGCG-----------------------------------------------CCGTCC`	`47 bp deleted → frameshift`
70	`CGGCGCGGACGCCATGGT-----------------------------CCCCGCGCCGTCC`	`29 bp deleted → frameshift`
69	`CGGCGCGGACGCCATGGTGC-----------------------------------CGTCC`	`35 bp deleted → frameshift`
69	`CGGC-----------------------------------AGCGCTGTCCCCGCGCCGTCC`	`35 bp deleted → frameshift`
68	`CGGCGC-------------------------------------------------CGTCC`	`49 bp deleted → frameshift`
66	`CGGCGCGGACGCCATG------------------------------TCCCCGCGCCGTCC`	`30 bp deleted → no frameshift`
66	`CGGCGCGGACGCCATGGTGCG------------------------------------TCC`	`36 bp deleted → no frameshift`
66	`CGGCGCGGACGCC------------------------------------CCGCGCCGTCC`	`36 bp deleted → no frameshift`
62	`CGGCGCGGACGCC-------------------------------------CGCGCCGTCC`	`37 bp deleted → frameshift`
60	`CGGCGCGGACGCC--------------------------------------GCGCCGTCC`	`38 bp deleted → frameshift`
54	`CGGCGCGGACGCCATGGTGCGGTTC----------------------------------C`	`34 bp deleted → frameshift`
54	`CGGC----------------------------------------TGTCCCCGCGCCGTCC`	`40 bp deleted → frameshift`
52	`CGGCGCGGACGCCATGGTGCGGT-----------------------------------CC`	`35 bp deleted → frameshift`
51	`CG-----------------------------------------CTGTCCCCGCGCCGTCC`	`41 bp deleted → frameshift`
42	`CGGCGCG---------------------------------------------CGCCGTCC`	`45 bp deleted → no frameshift`
40	`CGGCGCGGACGCCATGGT----------------------------------------CC`	`40 bp deleted → frameshift`
40	`CGGCGCGGACG----------------------------------------------TCC`	`46 bp deleted → frameshift`
38	`CGGCGCGGACGCC-----------------------------------------------`	`47 bp deleted → frameshift`
32	`CGGCGCG--------------------------------------------------TCC`	`50 bp deleted → frameshift`
32	`CG--------------------------------------------------CGCCGTCC`	`50 bp deleted → frameshift`
31	`CGGC---------------------------------------------------CGTCC`	`51 bp deleted → no frameshift`
29	`CGGCG----------------------------------------------------TCC`	`52 bp deleted → frameshift`
29	`CG----------------------------------------------------CCGTCC`	`52 bp deleted → frameshift`
25	`CG-------------------------------------------------------TCC`	`55 bp deleted → frameshift`

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