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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| mm4_intergenic_Gm26468|Rpsa-ps10_chr3_149981948_F | TCGTCGGCAGCGTCTGGCCTTGCTTAAGACACTGT | 59.8 | 0.80 |
| mm4_intergenic_Gm26468|Rpsa-ps10_chr3_149981948_R | GTCTCGTGGGCTCGGGCCTTTAGCATGGGGTATAGGA | 59.3 | 0.80 |
| mm3_exon_Arfgap3_chr15_83303163_F | TCGTCGGCAGCGTCATGACCCCATTGGCGAAGAC | 60.3 | 0.72 |
| mm3_exon_Arfgap3_chr15_83303163_R | GTCTCGTGGGCTCGGGAGGGGCAAGCTGACATCTT | 60.0 | 0.72 |
| mm4_intergenic_Gm25935|Gm22593_chr17_93507543_F | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intergenic_Gm25935|Gm22593_chr17_93507543_R | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intron_Rad51b_chr12_79385835_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intron_Rad51b_chr12_79385835_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Zfyve28|Rnf4_chr5_34295249_F | TCGTCGGCAGCGTCGCCACAGGTCTCCCAGTCTA | 60.6 | 0.31 |
| mm4_intergenic_Zfyve28|Rnf4_chr5_34295249_R | GTCTCGTGGGCTCGGGGGACAGAAAAGGCCAGACA | 59.8 | 0.31 |
| mm4_intergenic_Prr16|Gm25785_chr18_51386621_F | TCGTCGGCAGCGTCCCCTTCCATGTAAACATCCGGA | 60.0 | 0.31 |
| mm4_intergenic_Prr16|Gm25785_chr18_51386621_R | GTCTCGTGGGCTCGGCAAGGGACACAGGAGCCATT | 59.9 | 0.31 |
| mm4_intron_Pfkfb1_chrX_150603709_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intron_Pfkfb1_chrX_150603709_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Serp2|Lacc1_chr14_76615455_F | TCGTCGGCAGCGTCTTTGCCACTGACCTGCCTTT | 60.3 | 0.27 |
| mm4_intergenic_Serp2|Lacc1_chr14_76615455_R | GTCTCGTGGGCTCGGTGAAGGGCCATTTTGAAGCC | 59.0 | 0.27 |
| mm4_intergenic_Wnt4|Cdc42_chr4_137308679_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Wnt4|Cdc42_chr4_137308679_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm0_exon_Pop5_chr5_115238046_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm0_exon_Pop5_chr5_115238046_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Adcy5_chr16_35253894_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Adcy5_chr16_35253894_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm25758|Adamts3_chr5_89843551_F | TCGTCGGCAGCGTCGAAATGTTCAGCCACGCCC | 59.7 | 0.24 |
| mm4_intergenic_Gm25758|Adamts3_chr5_89843551_R | GTCTCGTGGGCTCGGGCGGTTTGTTTGACTTGCCT | 59.8 | 0.24 |
| mm2_intron_Cars2_chr8_11540098_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm2_intron_Cars2_chr8_11540098_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm3_intron_Pepd_chr7_34984699_F | TCGTCGGCAGCGTCCCGTGAATTGCTGGCAGTTT | 59.6 | 0.21 |
| mm3_intron_Pepd_chr7_34984699_R | GTCTCGTGGGCTCGGGAGTGTGAAGTGGGCGGTTA | 59.9 | 0.21 |
| mm4_intron_Ppp1r13b_chr12_111835882_F | TCGTCGGCAGCGTCGGACTTCCCACACCTGCATG | 60.9 | 0.20 |
| mm4_intron_Ppp1r13b_chr12_111835882_R | GTCTCGTGGGCTCGGTATCACACTGGGTTGCAGCC | 60.3 | 0.20 |
| mm3_intergenic_Gm17037|Gm23822_chr5_18788275_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intergenic_Gm17037|Gm23822_chr5_18788275_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Tnfsf9|Cd70_chr17_57134930_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Tnfsf9|Cd70_chr17_57134930_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Efcab6_chr15_83985224_F | TCGTCGGCAGCGTCATCTGCCTAAGTCCTGCGAG | 59.2 | 0.13 |
| mm4_intron_Efcab6_chr15_83985224_R | GTCTCGTGGGCTCGGTCAGTGTTGGGTGAGCCTTC | 59.8 | 0.13 |
| mm4_intergenic_Vmn1r184|Cyp2a4_chr7_26301720_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Vmn1r184|Cyp2a4_chr7_26301720_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Cyp2g1|Cyp2a5_chr7_26829865_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Cyp2g1|Cyp2a5_chr7_26829865_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Galnt9_chr5_110579459_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Galnt9_chr5_110579459_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Otud7a_chr7_63651527_F | TCGTCGGCAGCGTCAGCCTGATTCAGCCATGCTC | 60.4 | 0.07 |
| mm4_intron_Otud7a_chr7_63651527_R | GTCTCGTGGGCTCGGAGTGCCCATGACCAGCTTTT | 60.1 | 0.07 |
| mm4_intergenic_Gm11515|Tac4_chr11_95258445_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm11515|Tac4_chr11_95258445_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Erbb4_chr1_68643563_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Erbb4_chr1_68643563_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm25670|Gm26255_chr14_110618292_F | TCGTCGGCAGCGTCTGAGGGTGGTAAAACATGTGA | 57.1 | 0.07 |
| mm4_intergenic_Gm25670|Gm26255_chr14_110618292_R | GTCTCGTGGGCTCGGTTGCAGCTCTTGTGCATTGA | 58.6 | 0.07 |
| mm4_intergenic_Gm23698|Gm22186_chr10_110804467_F | TCGTCGGCAGCGTCAGCTGAAGGACCGTTCCATG | 60.0 | 0.06 |
| mm4_intergenic_Gm23698|Gm22186_chr10_110804467_R | GTCTCGTGGGCTCGGGGGTGTTCCAACAGTTGCTC | 59.3 | 0.06 |
| mm3_intergenic_Lce6a|Lce1a1_chr3_92635208_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intergenic_Lce6a|Lce1a1_chr3_92635208_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Id2|Gm22527_chr12_25389523_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Id2|Gm22527_chr12_25389523_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Greb1l_chr18_10531691_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Greb1l_chr18_10531691_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Fars2|Gm26395_chr13_36581207_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Fars2|Gm26395_chr13_36581207_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Nsmce2_chr15_59441521_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Nsmce2_chr15_59441521_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Dnah5_chr15_28306178_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Dnah5_chr15_28306178_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Tgfbi|SMAD5-AS1_1_chr13_56691568_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Tgfbi|SMAD5-AS1_1_chr13_56691568_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Kcnk10_chr12_98520746_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Kcnk10_chr12_98520746_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Smim13/Gm17364|Nedd9_chr13_41297934_F | TCGTCGGCAGCGTCGAGACCAAGCTCACCCACAT | 59.6 | 0.05 |
| mm4_intergenic_Smim13/Gm17364|Nedd9_chr13_41297934_R | GTCTCGTGGGCTCGGTACAGGCCAGCGTTTTCACA | 60.1 | 0.05 |
| mm4_intron_Tspan9_chr6_128009723_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Tspan9_chr6_128009723_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Csmd2|Hmgb4_chr4_128228729_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Csmd2|Hmgb4_chr4_128228729_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Scfd1|Coch_chr12_51494662_F | TCGTCGGCAGCGTCAGCAGCCAAACAGGGAAAGA | 59.8 | 0.04 |
| mm4_intergenic_Scfd1|Coch_chr12_51494662_R | GTCTCGTGGGCTCGGGACTTTAAGGCCCAGACCCC | 60.0 | 0.04 |
| mm4_intergenic_Gm24267|Gm22438_chr1_107851783_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24267|Gm22438_chr1_107851783_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Ofcc1_chr13_40057667_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Ofcc1_chr13_40057667_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Lcorl|Gm7931_chr5_46139971_F | TCGTCGGCAGCGTCTTTGTCTGGCTAGTGAGTTACA | 57.0 | 0.03 |
| mm4_intergenic_Lcorl|Gm7931_chr5_46139971_R | GTCTCGTGGGCTCGGACCTCTGCTGTCATTGCCAT | 59.6 | 0.03 |
| mm3_exon_Suclg1_chr6_73263939_F | TCGTCGGCAGCGTCCTTTTGCTGCTGCTGCCATT | 60.3 | 0.03 |
| mm3_exon_Suclg1_chr6_73263939_R | GTCTCGTGGGCTCGGATTAGCCTCGTCGTTCCCTG | 59.5 | 0.03 |
| mm4_intergenic_Smoc2|Thbs2_chr17_14521355_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Smoc2|Thbs2_chr17_14521355_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Cacnb1_chr11_98005174_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Cacnb1_chr11_98005174_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm24404|Nrg3_chr14_37509540_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm24404|Nrg3_chr14_37509540_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm8424|Gnl3l_chrX_150964449_F | TCGTCGGCAGCGTCAGAGGCCTCTACCAGTGACT | 59.2 | 0.02 |
| mm3_intergenic_Gm8424|Gnl3l_chrX_150964449_R | GTCTCGTGGGCTCGGGGGATTTTGGCCCATCATGG | 59.2 | 0.02 |
| mm4_intergenic_Gm24246|Pik3c3_chr18_29965496_F | TCGTCGGCAGCGTCGGAGCTCACTTTCTCTGGCA | 59.6 | 0.02 |
| mm4_intergenic_Gm24246|Pik3c3_chr18_29965496_R | GTCTCGTGGGCTCGGAGCAAGCCGAGTATGCTACG | 60.2 | 0.02 |
| mm4_exon_Tnfrsf1a_chr6_125357839_F | TCGTCGGCAGCGTCACCAGTTCCAACGCTACCTG | 59.9 | 0.01 |
| mm4_exon_Tnfrsf1a_chr6_125357839_R | GTCTCGTGGGCTCGGGAGGGGAGAGGACTTGGGT | 59.9 | 0.01 |
| mm4_intron_Cntnap2_chr6_46208333_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Cntnap2_chr6_46208333_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ndufb4|Fstl1_chr16_37765746_F | TCGTCGGCAGCGTCGGTGGTCATGGCTTTGTGAG | 59.1 | 0.01 |
| mm4_intergenic_Ndufb4|Fstl1_chr16_37765746_R | GTCTCGTGGGCTCGGAGTGGGCCTGTTATCAACCT | 58.6 | 0.01 |
| mm4_exon_Tmem206_chr1_191340857_F | TCGTCGGCAGCGTCGGAACTGCACCACTCAGAGG | 60.3 | 0.01 |
| mm4_exon_Tmem206_chr1_191340857_R | GTCTCGTGGGCTCGGGGGAAGAAAGGCTGGAGTCC | 60.0 | 0.01 |
| mm4_exon_D630003M21Rik_chr2_158216731_F | TCGTCGGCAGCGTCACAGGAGGGGAGAGAATGCT | 59.9 | 0.01 |
| mm4_exon_D630003M21Rik_chr2_158216731_R | GTCTCGTGGGCTCGGACTGAGGAAGAACCAGCAGC | 59.9 | 0.01 |
| mm4_exon_Sbds_chr5_130255269_F | TCGTCGGCAGCGTCCGAACTCTGGGCACAGGATT | 60.0 | 0.01 |
| mm4_exon_Sbds_chr5_130255269_R | GTCTCGTGGGCTCGGCCAACCAGATCCGACTGACC | 60.1 | 0.01 |
| mm4_intergenic_B230206L02Rik|Tob1_chr11_94163366_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_B230206L02Rik|Tob1_chr11_94163366_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ddx11|Soga2_chr17_66317750_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ddx11|Soga2_chr17_66317750_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Hey2|Gm24168_chr10_30871339_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Hey2|Gm24168_chr10_30871339_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Nckap5_chr1_126055746_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Nckap5_chr1_126055746_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Neurog2|Alpk1_chr3_127649761_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Neurog2|Alpk1_chr3_127649761_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm10309|Prkce_chr17_86533456_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm10309|Prkce_chr17_86533456_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Pdzrn4_chr15_92400275_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Pdzrn4_chr15_92400275_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm15405|Gm27039_chr17_92410408_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15405|Gm27039_chr17_92410408_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ust|Gm24374_chr10_8565515_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ust|Gm24374_chr10_8565515_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930555K19Rik|Oxr1_chr15_41206314_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930555K19Rik|Oxr1_chr15_41206314_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25570|Gm15631_chr6_105549982_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25570|Gm15631_chr6_105549982_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22789|Gm12003/Gm22801_chr11_12739979_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22789|Gm12003/Gm22801_chr11_12739979_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Grik5_chr7_25066307_F | TCGTCGGCAGCGTCGCCTGACTGGGTTGTTTGGA | 60.4 | 0.00 |
| mm4_intron_Grik5_chr7_25066307_R | GTCTCGTGGGCTCGGCTCAGTGGCGTGGTCTGATT | 60.0 | 0.00 |
| mm4_intron_Tenm2_chr11_36053845_F | TCGTCGGCAGCGTCTGCAAAGCCAGGCTCCATTA | 59.9 | 0.00 |
| mm4_intron_Tenm2_chr11_36053845_R | GTCTCGTGGGCTCGGCCTAGAAACCCACAGGCCAG | 60.0 | 0.00 |
| mm4_intron_RP23-448C3.1_chr19_22440063_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_RP23-448C3.1_chr19_22440063_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_D130052B06Rik_chr11_33611198_F | TCGTCGGCAGCGTCCTGAGCACACGGATTTCCCT | 60.0 | 0.00 |
| mm4_intron_D130052B06Rik_chr11_33611198_R | GTCTCGTGGGCTCGGAAGGCCTTGCACTGTGACAT | 60.1 | 0.00 |
| mm4_intergenic_Zkscan16|Ptgr1_chr4_58964371_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Zkscan16|Ptgr1_chr4_58964371_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ptprk|Themis_chr10_28654027_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ptprk|Themis_chr10_28654027_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Map1s|Mir1969_chr8_70919166_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Map1s|Mir1969_chr8_70919166_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Araf/Syn1_chrX_20860041_F | TCGTCGGCAGCGTCCTCCCCAAGATTGAGCGGAG | 60.1 | 0.00 |
| mm4_exon_Araf/Syn1_chrX_20860041_R | GTCTCGTGGGCTCGGTGGGAGTGAGGTCTAAGGCA | 59.8 | 0.00 |
| mm2_intergenic_Olfr921|Olfr922_chr9_38783508_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm2_intergenic_Olfr921|Olfr922_chr9_38783508_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm4_intergenic_Gm26468|Rpsa-ps10_chr3_149981948 |
TGGCCTTGCTTAAGACACTGTTTATGGTATAGTCCATAGAGCAGTTCAAGCAGGGTTATGCAATGAGTTTTCAGATTCCA TCCTATACCCCATGCTAAAGGC |
| mm3_exon_Arfgap3_chr15_83303163 |
ATGACCCCATTGGCGAAGACGGAAAGTTTTCCAGCAACGGAGCGCACACCTTGCTTGAACTGCGCCATGTCTGGGGCATT GGGCAGCACATTGGAAAGGTTGTAGTTTCCTGCACACAGAAAGATGTCAGCTTGCCCCTC |
| mm4_intergenic_Zfyve28|Rnf4_chr5_34295249 |
GCCACAGGTCTCCCAGTCTATGAGGCAATGTCCCAGAAAATGATCCTGGCCTCCCATGCTTGAATCTAACCATGTCAGCA TAGCTAAATGTCTGGCCTTTTCTGTCCC |
| mm4_intergenic_Prr16|Gm25785_chr18_51386621 |
CCCTTCCATGTAAACATCCGGAATTCTGTCTCTTCCTTATGCAGGGTGATGCTGCAGTTCAAGCAAGGCAGCCAGGACTA TAAATGGCTCCTGTGTCCCTTG |
| mm4_intergenic_Serp2|Lacc1_chr14_76615455 |
TTTGCCACTGACCTGCCTTTGAGAGGTTTAACATTACTGGTTTTCAGGGCCGTGCTTGAACCGTGCCTTTGCTGAATACC GTTTATTTACTTAGGCTTCAAAATGGCCCTTCA |
| mm4_intergenic_Gm25758|Adamts3_chr5_89843551 |
GAAATGTTCAGCCACGCCCAGAGAACCTGGGGAAAAAGCCATCCTCAGAGTCCCTGCTTGAACTCCACCAAGGAGGAGTT CATAAAGAGGAGTCCTCCATAAGCCAGTCATGTGGAACAAGGCAAGTCAAACAAACCGC |
| mm3_intron_Pepd_chr7_34984699 |
CCGTGAATTGCTGGCAGTTTGTGATGTAACCTCTCCCAGCTTGAACCCCACCATGACTGTGCTCCCACATCCTAACCGCC CACTTCACACTC |
| mm4_intron_Ppp1r13b_chr12_111835882 |
GGACTTCCCACACCTGCATGCTGATTAATCTGCTCAGAAGCCCACAGACCTTGCTTGAAGCCCACTGTGGCCAGCACTCA CCAACACGGCAAGGCTCTGCTGTGCAGCACGGGCTGCAACCCAGTGTGATA |
| mm4_intron_Efcab6_chr15_83985224 |
ATCTGCCTAAGTCCTGCGAGCCTGCGAGGCTGCTGGCCAGCGTGCTGCTCAAGCAAGGCAGGGGAGCTTGGTGAAGGCTC ACCCAACACTGA |
| mm4_intron_Otud7a_chr7_63651527 |
AGCCTGATTCAGCCATGCTCATCAGATCAGAGTTAACCTGGGTTCTTCCTTCTGAGTCATGATGCTGTTAAAGCAAGGAA AATGAAAAGCTGGTCATGGGCACT |
| mm4_intergenic_Gm25670|Gm26255_chr14_110618292 |
TGAGGGTGGTAAAACATGTGAATAGATTCCATGGTTGAACAGACCCATGGCTATCACAAAATATCAATGCACAAGAGCTG CAA |
| mm4_intergenic_Gm23698|Gm22186_chr10_110804467 |
AGCTGAAGGACCGTTCCATGGAGAGAAAGAATAAGAGCCATGATGCGGGACAAGAAGGGGGCCAGGAAGTTGGCAAGGGT GCTCTGAGCAACTGTTGGAACACCC |
| mm4_intergenic_Smim13/Gm17364|Nedd9_chr13_41297934 |
GAGACCAAGCTCACCCACATTTCTAACCCTGCTTGACTGGCACCTTGGCAATCAAAACAGTTCAGATTAAGTCTGTGAAA ACGCTGGCCTGTA |
| mm4_intergenic_Scfd1|Coch_chr12_51494662 |
AGCAGCCAAACAGGGAAAGACAGGCACGCAGCAGCCATGGAGCGGTACGAGAACGGCGCTGAACGTAAGTTAGCGGAGAA GGTCCAATAGTACCTGAGCGGGCACCGCCGTGGGGTCTGGGCCTTAAAGTC |
| mm4_intergenic_Lcorl|Gm7931_chr5_46139971 |
TTTGTCTGGCTAGTGAGTTACATTACCATGCTTTAGCCCCACCATCGCTAACAATGAGACAAAAAAGAAAATTTAATTGT TGATTTCAACATGACAGTCATATGGCAATGACAGCAGAGGT |
| mm3_exon_Suclg1_chr6_73263939 |
CTTTTGCTGCTGCTGCCATTAATGAAGCAATCGACGCGGAGATTCCCTTGGTTGTGTGCATTACGGAAGGTATTCCGCAG CAGGATATGGTGCGGGTCAAGCACAGACTGACACGCCAGGGAACGACGAGGCTAAT |
| mm3_intergenic_Gm8424|Gnl3l_chrX_150964449 |
AGAGGCCTCTACCAGTGACTTACTTCTTCCAGCAGACCTCCTTCAGGGTGCCATATATGGAGCCATGGCCATACATGAAC CCCACCATGGCTCTCCCATGATGGGCCAAAATCCC |
| mm4_intergenic_Gm24246|Pik3c3_chr18_29965496 |
GGAGCTCACTTTCTCTGGCATTTCCCTGCTTCAATCTCACCTTGGCTGCTCTGCCATAACACGTAGCATACTCGGCTTGC T |
| mm4_exon_Tnfrsf1a_chr6_125357839 |
ACCAGTTCCAACGCTACCTGAGTGAGACACACTTCCAGTGCGTGGACTGCAGCCCCTGCTTCAACGGCACCGTGACAATC CCCTGTGAGCACCGGTCACCCAAGTCCTCTCCCCTC |
| mm4_intergenic_Ndufb4|Fstl1_chr16_37765746 |
GGTGGTCATGGCTTTGTGAGCTTATTTCCCTTGCCAACATTATCTACCTATAAATGTCTACACCGTCCTCATGGAGTGCC CAGACCTGTACCACCACCCCTGCTTCATGTGCACCATGGCAGGTTGATAACAGGCCCACT |
| mm4_exon_Tmem206_chr1_191340857 |
GGAACTGCACCACTCAGAGGATCAATTACACCCACCCCTTCTTCAACCACACCATGGTAATGCCTGCTTCCCTGGGTTCT GGTAGCCACACACCCTGAGGACTCCAGCCTTTCTTCCC |
| mm4_exon_D630003M21Rik_chr2_158216731 |
ACAGGAGGGGAGAGAATGCTTGTCTTGGCCCAGGCCTGAGCCGAACCATGGCTTCTGGCATTCCCACAGGCTTTTGCATC TCAGAGGCTGCTGGTTCTTCCTCAGT |
| mm4_exon_Sbds_chr5_130255269 |
CGAACTCTGGGCACAGGATTACTCACACGCCACTCCGCCAGCCGACGACCTTGTTTTTATAGCAGGCGATTTCGAAGCGC TTCCCTCCCCGCTTCATCCGCACCACGGCCACATTGGTCAGTCGGATCTGGTTGG |
| mm4_intron_Grik5_chr7_25066307 |
GCCTGACTGGGTTGTTTGGAAAGCTTGCCTAGAATGTGTGAGGCCATGGTGGGGTTAATGGAGGGAAGGAGAGTGACACA GAGAACGGATAATAGAAATCAGACCACGCCACTGAG |
| mm4_intron_Tenm2_chr11_36053845 | TGCAAAGCCAGGCTCCATTATGGGGGCCATGGTGAGGAGCAAACATGGCTGGCCTGTGGGTTTCTAGG |
| mm4_intron_D130052B06Rik_chr11_33611198 |
CTGAGCACACGGATTTCCCTCTAAACAGGGGGAGCAGTGTGCACCATGCTGGAACAGCCCCATCGCTTAGTGATGTCACA GTGCAAGGCCTT |
| mm4_exon_Araf/Syn1_chrX_20860041 |
CTCCCCAAGATTGAGCGGAGTGCCTCCGAACCCTCCTTGCACCGCACCCAGGCTGATGAGTTGCCTGCTTGCCTTCTCAG CGCAGCCCGTCTTGTGCCTTAGACCTCACTCCCA |