CRISPOR

DNA break repair outcome predictions

Guide: CCGGCGCGGACGCCATGGTG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GTGCCGCCTTCCTCCCGCATCCGGCGCGGACGCCATGGTGCGGTTCAAGCACGGGTAAGCAGCGCTGTCCCCGCGCCGTC`	(Wild-type sequence)
389	`CCTCCCGCATCCGGCGCGGACGCCATGGT-----TCAAGCACGGGTAAGCAGCGCTGTCC`	`5 bp deleted → frameshift`
298	`CCTCCCGCATCCGGCGCGG--------------TTCAAGCACGGGTAAGCAGCGCTGTCC`	`14 bp deleted → frameshift`
242	`CCTCCCGCATCCGGCGCGGACG----------GTTCAAGCACGGGTAAGCAGCGCTGTCC`	`10 bp deleted → frameshift`
232	`CCTCCCGCATCCGG-------------------TTCAAGCACGGGTAAGCAGCGCTGTCC`	`19 bp deleted → frameshift`
213	`CCTCCCGCATCCGGCGCGGACGCCATGGT-----------------AAGCAGCGCTGTCC`	`17 bp deleted → frameshift`
179	`CCTCCCGCATCCGGCGCGGACGCCATGG----------------GTAAGCAGCGCTGTCC`	`16 bp deleted → frameshift`
179	`CCTCCCGCATCCGGCG----------------GTTCAAGCACGGGTAAGCAGCGCTGTCC`	`16 bp deleted → frameshift`
175	`CCTCCCGCATCCGGCGCGGACG---------------------GGTAAGCAGCGCTGTCC`	`21 bp deleted → no frameshift`
172	`CCTCCCGCATCCGGCGCGG-------------------------GTAAGCAGCGCTGTCC`	`25 bp deleted → frameshift`
170	`CCTCCCGCATCCGGCGCGGACGC-----------------ACGGGTAAGCAGCGCTGTCC`	`17 bp deleted → frameshift`
164	`CCTCCCGCATCCGGCGCGGACGCCA------------AGCACGGGTAAGCAGCGCTGTCC`	`12 bp deleted → no frameshift`
134	`CCTCCCGCATCCGGCGCGGACGCCA----------------CGGGTAAGCAGCGCTGTCC`	`16 bp deleted → frameshift`
133	`CCTCCCGCATCCGG------------------------------GTAAGCAGCGCTGTCC`	`30 bp deleted → no frameshift`
126	`CCTCCCGCATCCGGCGC-----------------------ACGGGTAAGCAGCGCTGTCC`	`23 bp deleted → frameshift`
121	`CCTCCCGCATCCGGCGCGGACGC--------------------------------TGTCC`	`32 bp deleted → frameshift`
120	`CCTCCCGCATCCGGCGC--------------------------------------TGTCC`	`38 bp deleted → frameshift`
114	`CCTCCCGCATCCGGC-------------------------ACGGGTAAGCAGCGCTGTCC`	`25 bp deleted → frameshift`
114	`CCTCCCG-------------------------GTTCAAGCACGGGTAAGCAGCGCTGTCC`	`25 bp deleted → frameshift`
109	`CCTCCCGCATCCGGCGCGG--------------------------TAAGCAGCGCTGTCC`	`26 bp deleted → frameshift`
103	`CCTCCCGCATCCGGCGCGGACGC---------------------------AGCGCTGTCC`	`27 bp deleted → no frameshift`
103	`CCTCCCGCATCCGGCG---------------------------GGTAAGCAGCGCTGTCC`	`27 bp deleted → no frameshift`
101	`CCTCCCGCA--------------------------------CGGGTAAGCAGCGCTGTCC`	`32 bp deleted → frameshift`
99	`CCTCCCGCATCCGGCGCG------------------------------------CTGTCC`	`36 bp deleted → no frameshift`
89	`CCTCCCGCATCCGGCGCGGACGC------------------------------GCTGTCC`	`30 bp deleted → no frameshift`
86	`CCTCCCGCATC-------------------------AAGCACGGGTAAGCAGCGCTGTCC`	`25 bp deleted → frameshift`
84	`CCTCCCGCATCCGG-------------------------------TAAGCAGCGCTGTCC`	`31 bp deleted → frameshift`
81	`CCTCCCGCATCCGGCGCGGACGCCA--------------------------GCGCTGTCC`	`26 bp deleted → frameshift`
77	`CCTCCCGCATCCGGCGCGGACGCCATGGTG---------------------------TCC`	`27 bp deleted → no frameshift`
76	`CCTCCCGCATCCGGCGC---------------------------------AGCGCTGTCC`	`33 bp deleted → no frameshift`
74	`CCTCCCGCA----------------------------AGCACGGGTAAGCAGCGCTGTCC`	`28 bp deleted → frameshift`
70	`CCTCCCGCATCCGGCGCGGACGCCATGGT-----------------------------CC`	`29 bp deleted → frameshift`
69	`CCTCCCGCATCCGGC-----------------------------------AGCGCTGTCC`	`35 bp deleted → frameshift`
66	`CCTCCCGCATCCGGCGCGGACGCCATG------------------------------TCC`	`30 bp deleted → no frameshift`
66	`CCTCCCGCATCCGGCGCGGACGCC------------------------------------`	`36 bp deleted → no frameshift`
66	`CCTCCCG------------------------------------GGTAAGCAGCGCTGTCC`	`36 bp deleted → no frameshift`
61	`CCTCCCGCA------------------------------------------GCGCTGTCC`	`42 bp deleted → no frameshift`
60	`CCTC--------------------------------AAGCACGGGTAAGCAGCGCTGTCC`	`32 bp deleted → frameshift`
57	`CCTCCCGC-----------------------------------------------TGTCC`	`47 bp deleted → frameshift`
54	`CCTCCCGCATCCGGC----------------------------------------TGTCC`	`40 bp deleted → frameshift`
51	`CCTCCCGCATCCG-----------------------------------------CTGTCC`	`41 bp deleted → frameshift`
45	`CCTCCCGCATCC------------------------------------------------`	`48 bp deleted → no frameshift`
42	`CCTCCCGC---------------------------------------------GCTGTCC`	`45 bp deleted → no frameshift`
32	`CCTCC-------------------------------------------------------`	`55 bp deleted → frameshift`
26	`CCTCCC------------------------------------------------------`	`54 bp deleted → no frameshift`
22	`CC----------------------------------------------------------`	`58 bp deleted → frameshift`
21	`CCT-----------------------------------------------------GTCC`	`53 bp deleted → frameshift`

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