CRISPOR

DNA break repair outcome predictions

Guide: CCCGTGCTTGAACCGCACCA TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGCGGGGACAGCGCTGCTTACCCGTGCTTGAACCGCACCATGGCGTCCGCGCCGGATGCGGGAGGAAGGCGGCACTAGGC`	(Wild-type sequence)
397	`GCGCTGCTTACCCGTGCTTGAACCGC--------------GCCGGATGCGGGAGGAAGGC`	`14 bp deleted → frameshift`
269	`GCGCTGCTTACCCGTGCTTGAACCGC----------------CGGATGCGGGAGGAAGGC`	`16 bp deleted → frameshift`
268	`GCGCTGCTTACCCGTGCTTGAACCGC--------GTCCGCGCCGGATGCGGGAGGAAGGC`	`8 bp deleted → frameshift`
255	`GCGCTGCTTACCCGTGCTTGAACCGCACC---------GCGCCGGATGCGGGAGGAAGGC`	`9 bp deleted → no frameshift`
242	`GCGCTGCTTACCCGTGCTTGAACCG----------TCCGCGCCGGATGCGGGAGGAAGGC`	`10 bp deleted → frameshift`
232	`GCGCTGCTTACCCGTGCTTGAACCG-------------------GATGCGGGAGGAAGGC`	`19 bp deleted → frameshift`
198	`GCGCTGCTTACCCGTGCTTGAACCGCACC--------------GGATGCGGGAGGAAGGC`	`14 bp deleted → frameshift`
175	`GCGCTGCTTACCCGT---------------------CCGCGCCGGATGCGGGAGGAAGGC`	`21 bp deleted → no frameshift`
172	`GCGCTGCTTACCCG-------------------------CGCCGGATGCGGGAGGAAGGC`	`25 bp deleted → frameshift`
170	`GCGCTGCTTACCCGTGC-----------------GTCCGCGCCGGATGCGGGAGGAAGGC`	`17 bp deleted → frameshift`
164	`GCGCTGCTTACCCGTGCTTG------------GCGTCCGCGCCGGATGCGGGAGGAAGGC`	`12 bp deleted → no frameshift`
134	`GCGCTGCTTACCCGTG----------------GCGTCCGCGCCGGATGCGGGAGGAAGGC`	`16 bp deleted → frameshift`
133	`GCGCTGCTTACCCG------------------------------GATGCGGGAGGAAGGC`	`30 bp deleted → no frameshift`
126	`GCGCTGCTTACCCGTGCTTGAACCGC-----------------------GGGAGGAAGGC`	`23 bp deleted → frameshift`
126	`GCGCTGCTTACCCGTGC-----------------------GCCGGATGCGGGAGGAAGGC`	`23 bp deleted → frameshift`
121	`GCG--------------------------------TCCGCGCCGGATGCGGGAGGAAGGC`	`32 bp deleted → frameshift`
120	`GCGC--------------------------------------CGGATGCGGGAGGAAGGC`	`38 bp deleted → frameshift`
114	`GCGCTGCTTACCCGTGCTTGAACCG-------------------------GGAGGAAGGC`	`25 bp deleted → frameshift`
114	`GCGCTGCTTACCCGTGC-------------------------CGGATGCGGGAGGAAGGC`	`25 bp deleted → frameshift`
109	`GCGCTGCTTACC--------------------------GCGCCGGATGCGGGAGGAAGGC`	`26 bp deleted → frameshift`
103	`GCGCTGCTTACCCG---------------------------CCGGATGCGGGAGGAAGGC`	`27 bp deleted → no frameshift`
103	`GCGCTGC---------------------------GTCCGCGCCGGATGCGGGAGGAAGGC`	`27 bp deleted → no frameshift`
101	`GCGCTGCTTACCCGTGC--------------------------------GGGAGGAAGGC`	`32 bp deleted → frameshift`
99	`GCGC------------------------------------GCCGGATGCGGGAGGAAGGC`	`36 bp deleted → no frameshift`
89	`GCGC------------------------------GTCCGCGCCGGATGCGGGAGGAAGGC`	`30 bp deleted → no frameshift`
86	`GCGCTGCTTACCCGTGCTTGA-------------------------TGCGGGAGGAAGGC`	`25 bp deleted → frameshift`
84	`GCGCTGCTTACC-------------------------------GGATGCGGGAGGAAGGC`	`31 bp deleted → frameshift`
81	`GCGCTG--------------------------GCGTCCGCGCCGGATGCGGGAGGAAGGC`	`26 bp deleted → frameshift`
76	`GCGCTGC---------------------------------GCCGGATGCGGGAGGAAGGC`	`33 bp deleted → no frameshift`
74	`GCGCTGCTTACCCGTGCTTG----------------------------CGGGAGGAAGGC`	`28 bp deleted → frameshift`
73	`GCGCTGCTTACCCGTGCTTGAACCGC----------------------------------`	`34 bp deleted → frameshift`
69	`GCGCTGCTTACCCGTGCTTGAA-----------------------------------GGC`	`35 bp deleted → frameshift`
69	`GCGCTGC-----------------------------------CGGATGCGGGAGGAAGGC`	`35 bp deleted → frameshift`
66	`GCGCTGCTTACCCG------------------------------------GGAGGAAGGC`	`36 bp deleted → no frameshift`
61	`GCGCTGC------------------------------------------GGGAGGAAGGC`	`42 bp deleted → no frameshift`
60	`GCGCTGCTTACCCGTGCTTGA--------------------------------GGAAGGC`	`32 bp deleted → frameshift`
57	`GCG-----------------------------------------------GGAGGAAGGC`	`47 bp deleted → frameshift`
54	`GC----------------------------------------CGGATGCGGGAGGAAGGC`	`40 bp deleted → frameshift`
51	`GCG-----------------------------------------GATGCGGGAGGAAGGC`	`41 bp deleted → frameshift`
46	`GCGCTGCTTACCCGTGC-------------------------------------------`	`43 bp deleted → frameshift`
42	`GCGC---------------------------------------------GGGAGGAAGGC`	`45 bp deleted → no frameshift`
28	`GCGCTGC-----------------------------------------------------`	`53 bp deleted → frameshift`
24	`GCGC--------------------------------------------------------`	`56 bp deleted → frameshift`
22	`GC----------------------------------------------------------`	`58 bp deleted → frameshift`

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