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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| mm0_exon_Pop5_chr5_115238021_F | TCGTCGGCAGCGTCGAGCGTGGAGATTGACAGGG | 60.4 | 1.00 |
| mm0_exon_Pop5_chr5_115238021_R | GTCTCGTGGGCTCGGTTACCTGTGCTTGAACCGCA | 60.1 | 1.00 |
| mm4_intron_Baiap2l2_chr15_79268644_F | TCGTCGGCAGCGTCGTGGCTCTATTAAGGATGTTTCAAA | 57.6 | 0.46 |
| mm4_intron_Baiap2l2_chr15_79268644_R | GTCTCGTGGGCTCGGCCCTGCAGAAGTGAGTTCCA | 59.6 | 0.46 |
| mm4_intron_Grik1_chr16_87970075_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intron_Grik1_chr16_87970075_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Hp|Dhodh_chr8_109590691_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Hp|Dhodh_chr8_109590691_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Ldlrad3|Gm13866_chr2_102186608_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Ldlrad3|Gm13866_chr2_102186608_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Med9_chr11_59957602_F | TCGTCGGCAGCGTCGACAGAGACAGGCAAATGGC | 59.1 | 0.16 |
| mm4_intron_Med9_chr11_59957602_R | GTCTCGTGGGCTCGGAGCACACCTTCAGGCTTGTT | 60.1 | 0.16 |
| mm4_intron_Ms4a15_chr19_10980393_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Ms4a15_chr19_10980393_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Tcf12|Gm25045_chr9_72112102_F | TCGTCGGCAGCGTCCTGGGCAGCATCTGGATCTG | 60.5 | 0.15 |
| mm4_intergenic_Tcf12|Gm25045_chr9_72112102_R | GTCTCGTGGGCTCGGTTGCGCTTTTCCCACAGAGT | 60.4 | 0.15 |
| mm4_intron_Clip2_chr5_134534187_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Clip2_chr5_134534187_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm26005|Epcam_chr17_87477951_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm26005|Epcam_chr17_87477951_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm13188|1700080N15Rik_chr2_4089457_F | TCGTCGGCAGCGTCCCAGCCTGGGCAGTAACATA | 59.4 | 0.13 |
| mm4_intergenic_Gm13188|1700080N15Rik_chr2_4089457_R | GTCTCGTGGGCTCGGTGGGTGGCTCTCAAAACTGT | 59.4 | 0.13 |
| mm4_intergenic_Grpr|Rnf138rt1_chrX_163553057_F | TCGTCGGCAGCGTCTCAGCATGACAGTCCATGTTCT | 59.6 | 0.11 |
| mm4_intergenic_Grpr|Rnf138rt1_chrX_163553057_R | GTCTCGTGGGCTCGGGCCATGCTGCTTGCTTAAGG | 60.1 | 0.11 |
| mm4_intergenic_Gm22845|Nt5c1b_chr12_10157767_F | TCGTCGGCAGCGTCTGCAGTTTGCTGACTTGCAC | 59.8 | 0.11 |
| mm4_intergenic_Gm22845|Nt5c1b_chr12_10157767_R | GTCTCGTGGGCTCGGCCAGTAGAAGAAGGTGCGGG | 60.1 | 0.11 |
| mm4_exon_Acaca_chr11_84391631_F | TCGTCGGCAGCGTCTGCAGTTTGCTGACTTGCAC | 59.8 | 0.11 |
| mm4_exon_Acaca_chr11_84391631_R | GTCTCGTGGGCTCGGCAGCTTGTGCGCCTATGTCA | 61.0 | 0.11 |
| mm4_intron_Abhd6_chr14_8027554_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Abhd6_chr14_8027554_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Pou3f1_chr4_124660183_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Pou3f1_chr4_124660183_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Fhad1_chr4_141930228_F | TCGTCGGCAGCGTCTCCAAGGTCCTGGCTCAGTA | 59.8 | 0.09 |
| mm4_intron_Fhad1_chr4_141930228_R | GTCTCGTGGGCTCGGCCCGTTGTGGACTCTAGTGG | 59.7 | 0.09 |
| mm4_intergenic_Gm2366/Fat1|Gm2366_chr8_45020783_F | TCGTCGGCAGCGTCAGGAGGAAAGAGAACACGTGT | 58.9 | 0.09 |
| mm4_intergenic_Gm2366/Fat1|Gm2366_chr8_45020783_R | GTCTCGTGGGCTCGGTTCGTTCACCAGAATGCCCC | 60.6 | 0.09 |
| mm4_intergenic_Klrb1b|Gm26656_chr6_128842798_F | TCGTCGGCAGCGTCCGATCTTTCCTGGAGTCCCG | 59.8 | 0.09 |
| mm4_intergenic_Klrb1b|Gm26656_chr6_128842798_R | GTCTCGTGGGCTCGGCCTCAGGGATGACTGACACG | 59.8 | 0.09 |
| mm4_intron_Ptprn2_chr12_116568559_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Ptprn2_chr12_116568559_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_P4ha2|Gm12221_chr11_54133526_F | TCGTCGGCAGCGTCACCCACACTGACCAGGAGTA | 59.8 | 0.08 |
| mm4_intergenic_P4ha2|Gm12221_chr11_54133526_R | GTCTCGTGGGCTCGGCACACCCAGCTAGTTAAAACCT | 58.2 | 0.08 |
| mm4_intergenic_Nbas|Gm4929_chr12_13595239_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Nbas|Gm4929_chr12_13595239_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Skiv2l_chr17_34846457_F | TCGTCGGCAGCGTCCCCATCTCAGCCTCTCTCCT | 60.1 | 0.06 |
| mm4_exon_Skiv2l_chr17_34846457_R | GTCTCGTGGGCTCGGCATTAGCCTGCCCTCCTCAG | 59.8 | 0.06 |
| mm4_intergenic_Rasa1|Cox7c_chr13_85531879_F | TCGTCGGCAGCGTCTTTCCCACTGTCCCCTCCAT | 60.4 | 0.06 |
| mm4_intergenic_Rasa1|Cox7c_chr13_85531879_R | GTCTCGTGGGCTCGGAAGGCATGATAGCAAAGAGGA | 57.0 | 0.06 |
| mm4_intergenic_Gm5250|Prdm14_chr1_13095998_F | TCGTCGGCAGCGTCTGATGCAGCTGGAAGATATGAGA | 59.3 | 0.06 |
| mm4_intergenic_Gm5250|Prdm14_chr1_13095998_R | GTCTCGTGGGCTCGGGGGCCCTCTTTCTGCTTTTG | 59.3 | 0.06 |
| mm3_intergenic_Tex10|Msantd3_chr4_48473537_F | TCGTCGGCAGCGTCGTCCAGATTTGCACGCCAC | 59.7 | 0.06 |
| mm3_intergenic_Tex10|Msantd3_chr4_48473537_R | GTCTCGTGGGCTCGGAGCCAAGGATGCTGGAAGAC | 60.0 | 0.06 |
| mm4_exon_Prss48_chr3_85997271_F | TCGTCGGCAGCGTCATCCTTCCTCGACTGCCTCT | 60.0 | 0.05 |
| mm4_exon_Prss48_chr3_85997271_R | GTCTCGTGGGCTCGGAGTGAGGCGTGTGAACAACT | 59.8 | 0.05 |
| mm4_exon_Ergic3_chr2_156008077_F | TCGTCGGCAGCGTCCTTTGAGGAGGCAGGTGAGG | 60.0 | 0.05 |
| mm4_exon_Ergic3_chr2_156008077_R | GTCTCGTGGGCTCGGGGAAAGGGGACACCAGCC | 59.9 | 0.05 |
| mm3_intron_D630045J12Rik_chr6_38210316_F | TCGTCGGCAGCGTCTTCCAGCGCTGCCTCATA | 58.6 | 0.05 |
| mm3_intron_D630045J12Rik_chr6_38210316_R | GTCTCGTGGGCTCGGAGCAGGAGACACACCCTGA | 60.1 | 0.05 |
| mm4_intergenic_Ighv12-3|Ighv6-3_chr12_114391534_F | TCGTCGGCAGCGTCGCTTGAGACAGAAACAGCCC | 59.1 | 0.04 |
| mm4_intergenic_Ighv12-3|Ighv6-3_chr12_114391534_R | GTCTCGTGGGCTCGGTTACAGGGATCTGGGCAGGT | 60.2 | 0.04 |
| mm4_exon_Anapc1_chr2_128687285_F | TCGTCGGCAGCGTCGTCACGATGACTCCTCACCG | 60.1 | 0.04 |
| mm4_exon_Anapc1_chr2_128687285_R | GTCTCGTGGGCTCGGGCAGGTCGGGAAATCCAAGA | 60.0 | 0.04 |
| mm4_intron_Rbfox3_chr11_118577556_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Rbfox3_chr11_118577556_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Ankmy2_chr12_36159897_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Ankmy2_chr12_36159897_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Brd2_chr17_34112547_F | TCGTCGGCAGCGTCAGCAAGGGAAAGTGGGGAAG | 59.8 | 0.03 |
| mm4_exon_Brd2_chr17_34112547_R | GTCTCGTGGGCTCGGGCGATTCAGACTCGGGCTAA | 59.8 | 0.03 |
| mm4_intron_Sept11_chr5_93133971_F | TCGTCGGCAGCGTCTCAGTTTCCCCTGCAGTGTC | 59.8 | 0.03 |
| mm4_intron_Sept11_chr5_93133971_R | GTCTCGTGGGCTCGGGGGCTGCAGTTAATGGTCCT | 60.0 | 0.03 |
| mm4_intergenic_Nfia|Nfia/E130114P18Rik_chr4_97775151_F | TCGTCGGCAGCGTCGCTGTAGTTGGAACTGGGCT | 59.9 | 0.03 |
| mm4_intergenic_Nfia|Nfia/E130114P18Rik_chr4_97775151_R | GTCTCGTGGGCTCGGCAAGTTCAGCACCAAAGCCC | 59.9 | 0.03 |
| mm4_exon_Ndc1_chr4_107415950_F | TCGTCGGCAGCGTCCCTGGAAGGTGAGGGGTCAT | 60.9 | 0.02 |
| mm4_exon_Ndc1_chr4_107415950_R | GTCTCGTGGGCTCGGAACTTCACCAAAGCCAACGC | 59.8 | 0.02 |
| mm4_intergenic_1500009L16Rik|Nuak1_chr10_84029650_F | TCGTCGGCAGCGTCGCCTTCTGAGCTGTGTGGAT | 60.0 | 0.02 |
| mm4_intergenic_1500009L16Rik|Nuak1_chr10_84029650_R | GTCTCGTGGGCTCGGCGAGGCTTCACACAGCTGTA | 60.0 | 0.02 |
| mm4_exon_Taf10_chr7_105744052_F | TCGTCGGCAGCGTCCGGCACCTAGTCCGAGATG | 59.6 | 0.02 |
| mm4_exon_Taf10_chr7_105744052_R | GTCTCGTGGGCTCGGTGGCTGGAGTCGCCACTG | 62.0 | 0.02 |
| mm4_intergenic_Ppp1r15b|Plekha6_chr1_133192455_F | TCGTCGGCAGCGTCCTCTCTTATGGCTGCTCGGG | 59.9 | 0.01 |
| mm4_intergenic_Ppp1r15b|Plekha6_chr1_133192455_R | GTCTCGTGGGCTCGGCCCAGCCCCACATGAATTGA | 60.3 | 0.01 |
| mm4_intron_Gm15261_chrX_169101930_F | TCGTCGGCAGCGTCTGGAGGTTGAGTGGGAATTGG | 59.9 | 0.01 |
| mm4_intron_Gm15261_chrX_169101930_R | GTCTCGTGGGCTCGGGTCCTGAGCCAAATCTGCCA | 60.3 | 0.01 |
| mm4_intergenic_AU022754|Mirlet7c-2_chr15_85656262_F | TCGTCGGCAGCGTCGCCAGGCTCCCAACTTGTAA | 60.2 | 0.00 |
| mm4_intergenic_AU022754|Mirlet7c-2_chr15_85656262_R | GTCTCGTGGGCTCGGACAGCCTTCACAGCTCTGAC | 59.9 | 0.00 |
| mm4_intergenic_Gtl3|Csnk2a2_chr8_95434928_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gtl3|Csnk2a2_chr8_95434928_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25672|Barhl2_chr5_106227956_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25672|Barhl2_chr5_106227956_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930583I09Rik|Gm25344_chr17_64913364_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930583I09Rik|Gm25344_chr17_64913364_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Crebzf_chr7_90442862_F | TCGTCGGCAGCGTCGAGAGTAACTTCCGGCCAGG | 59.8 | 0.00 |
| mm4_exon_Crebzf_chr7_90442862_R | GTCTCGTGGGCTCGGCAAGGTCCGAGTGGCCTC | 59.7 | 0.00 |
| mm4_intron_Cntnap2_chr6_46405781_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cntnap2_chr6_46405781_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Usp12_chr5_146754760_F | TCGTCGGCAGCGTCGGAGAACAGACAACTACGGCA | 59.9 | 0.00 |
| mm4_intron_Usp12_chr5_146754760_R | GTCTCGTGGGCTCGGGGTGGTGAAGATCAGCTGCT | 60.0 | 0.00 |
| mm4_intron_Arf3_chr15_98762304_F | TCGTCGGCAGCGTCAACTCAGGGCCAGTGACAAG | 59.8 | 0.00 |
| mm4_intron_Arf3_chr15_98762304_R | GTCTCGTGGGCTCGGGAGCTAGCTCGGAGGAGAGA | 59.8 | 0.00 |
| mm4_intergenic_Tbc1d22b/RP24-271E12.4|Tbc1d22b_chr17_29559923_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Tbc1d22b/RP24-271E12.4|Tbc1d22b_chr17_29559923_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_P2ry6|P2ry2_chr7_100994277_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_P2ry6|P2ry2_chr7_100994277_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Hipk3_chr2_104493370_F | TCGTCGGCAGCGTCTAACGAGAGAAGCCGGCAAC | 60.3 | 0.00 |
| mm4_exon_Hipk3_chr2_104493370_R | GTCTCGTGGGCTCGGTGTCAACTCTGGAGACGTGC | 59.9 | 0.00 |
| mm4_exon_Ctif_chr18_75432159_F | TCGTCGGCAGCGTCTCATCAGAGGCAGGCTTTGA | 59.0 | 0.00 |
| mm4_exon_Ctif_chr18_75432159_R | GTCTCGTGGGCTCGGAAAGGCTGAGCACCTTCCTG | 60.2 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm0_exon_Pop5_chr5_115238021 |
GAGCGTGGAGATTGACAGGGCACGCTGCCTGTCCGAGCCTAGTGCCGCCTTCCTCCCGCATCCGGCGCGGACGCCATGGT GCGGTTCAAGCACAGGTAA |
| mm4_intron_Baiap2l2_chr15_79268644 |
GTGGCTCTATTAAGGATGTTTCAAAGAATAGCCTCCTTCCTCCAGCAACCAGCAGGGATTGGACATCCAGACAATGGAAC TCACTTCTGCAGGG |
| mm4_intron_Med9_chr11_59957602 |
GACAGAGACAGGCAAATGGCTCGCCCACTGTGGATGCTGGAGGAAGGGCTAGACACAGGAGAGGGGGATGTGGGAGTTGC TGGGAGTGAGCAAACAAGCCTGAAGGTGTGCT |
| mm4_intergenic_Tcf12|Gm25045_chr9_72112102 |
CTGGGCAGCATCTGGATCTGCCACCCGCAGCCATTCCGGAGCTCAGCCACGCCGGACGCGGGAGGCGAGGCCGGCTGTGG AGCCGCAGCTGTGGTGGGCGGACTCTGTGGGAAAAGCGCAA |
| mm4_intergenic_Gm13188|1700080N15Rik_chr2_4089457 |
CCAGCCTGGGCAGTAACATAACTGAGCCCAGGCATTCCTCCTGGATCCGGAGTGGAGTCATCCCTCCTTTGTGAAAAATC CTTCTCTTGAGAAAAACTAATCTGCAGTCTGACAGTTTTGAGAGCCACCCA |
| mm4_intergenic_Grpr|Rnf138rt1_chrX_163553057 |
TCAGCATGACAGTCCATGTTCTGCCCCTTCTGATGAGGGAGGAAGGAAAGGAACATGTATAAGAATTTAGATTACTGCTA TGGGTTCATGTTACCCAAGAGTAAATCCTTAAGCAAGCAGCATGGC |
| mm4_intergenic_Gm22845|Nt5c1b_chr12_10157767 |
TGCAGTTTGCTGACTTGCACGACACACCAGGCCGGATGCAGGAGAAGGGTGTCATTAACGATATCTTGGATTGGAAAACA TCCCGCACCTTCTTCTACTGG |
| mm4_exon_Acaca_chr11_84391631 |
TGCAGTTTGCTGACTTGCACGACACACCAGGCCGGATGCAGGAGAAGGGTGTCATTAACGTAAGTAACACAATTGCCATT TCTGACTTGTCAAAGCCTTGTAAGTGACATAGGCGCACAAGCTG |
| mm4_intron_Fhad1_chr4_141930228 |
TCCAAGGTCCTGGCTCAGTAGCCAAAATCCCAAGAAAAGCCTCGCCAGCATTCACAGGGCTTCCTCCGGCACCCGGCAGG GCATCGGGTGTTTGGAACTTACAACAAGATTTCCACTAGAGTCCACAACGGG |
| mm4_intergenic_Gm2366/Fat1|Gm2366_chr8_45020783 |
AGGAGGAAAGAGAACACGTGTATAATAAAAAATAAACGTAGCCACACCGGATAGGGAAGGAAGGAGGAGTCTTCAGGCAG GGGCATTCTGGTGAACGAA |
| mm4_intergenic_Klrb1b|Gm26656_chr6_128842798 |
CGATCTTTCCTGGAGTCCCGCACGTGGACAAAAACCAGGGGCAAGCTTCCAAGTGCTCCCAGCAGGATGCGGGAGGATTG AGTTTTGAGACGTGTCAGTCATCCCTGAGG |
| mm4_intergenic_P4ha2|Gm12221_chr11_54133526 |
ACCCACACTGACCAGGAGTAAATGAACGTTCTTACCCTACCTCCCTCCAGCATCTGGTGTGGTCCTATGCTTAGGTTTTA ACTAGCTGGGTGTG |
| mm4_exon_Skiv2l_chr17_34846457 |
CCCATCTCAGCCTCTCTCCTGAACCACTTATCTACAGGGTAAGGCATAAGGCAGAGGGGTGGGAGGTAAGGTGAGGCCCA GGAAGGATGCAGGAGGAAGGCAGGCAGGCTGAGGAGGGCAGGCTAATG |
| mm4_intergenic_Rasa1|Cox7c_chr13_85531879 | TTTCCCACTGTCCCCTCCATCCTTCCTCTCCCATCCTGCTTGGTCCTCTTTGCTATCATGCCTT |
| mm4_intergenic_Gm5250|Prdm14_chr1_13095998 |
TGATGCAGCTGGAAGATATGAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCAAAAGCAGAAAGAGGGCCC |
| mm3_intergenic_Tex10|Msantd3_chr4_48473537 |
GTCCAGATTTGCACGCCACGCCCCCTCTAAGAGGGGCTGCGCCGGAAGCTGGAGGAAAGAGAGGAGGGTCTAGGGTCCAG CAAGCCGAGTCAGGTCTTCCAGCATCCTTGGCT |
| mm4_exon_Prss48_chr3_85997271 |
ATCCTTCCTCGACTGCCTCTCACCAGCACAGAACATGTCTTCCTTGATGACCCGCTCCAGATCCGGGAGGAAGACACCAA TTGGGTTGTAGAGTTGTTCACACGCCTCACT |
| mm4_exon_Ergic3_chr2_156008077 |
CTTTGAGGAGGCAGGTGAGGGGGTGGAGCCCCACTTACGCACCGCTACGCCTGCGCAAGACAGCGAGGGGCGTGACCCCG GGTCCAGGCCGGAAGCGGGAGGCTGGAGGGGCGGGCCGGCTGGTGTCCCCTTTCC |
| mm3_intron_D630045J12Rik_chr6_38210316 |
TTCCAGCGCTGCCTCATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNATGCGGGAGGTAGATCAGGGTGTGTCTCCTGCT |
| mm4_intergenic_Ighv12-3|Ighv6-3_chr12_114391534 |
GCTTGAGACAGAAACAGCCCTTTCTTTTCTCAGATGCTGACTTGTTCAATTACATGATGCCACTCCAGATGGTGGAGGAA GGAAAGGGACTACTGAGAAAGTGGTCTCACCTGCCCAGATCCCTGTAA |
| mm4_exon_Anapc1_chr2_128687285 |
GTCACGATGACTCCTCACCGCCCCAGTTTCTCATCCCTTTTCCCTCCCGGATGCCGGAGGAAGAATGGGCGGCAGCGGGA CACCAAAGACTTGCGAACAACTCAAATGGCTGGAGCCATCTTGGATTTCCCGACCTGC |
| mm4_exon_Brd2_chr17_34112547 |
AGCAAGGGAAAGTGGGGAAGGGTGGTTCAGGGGTCCGGTCCTGCGGAGCCTTCCTGCCCCATCTGGCCTGGCCCCTTAGC CCGAGTCTGAATCGC |
| mm4_intron_Sept11_chr5_93133971 |
TCAGTTTCCCCTGCAGTGTCTCGCAGCAGTCACGGAACTAATTTATTTCAGGTTTTACAATTGGAGCTGTCCCTTCCTCC CGCCTCACTCGGGGAAAGGAAACTCATGTTTCCTGAGGACCATTAACTGCAGCCC |
| mm4_intergenic_Nfia|Nfia/E130114P18Rik_chr4_97775151 |
GCTGTAGTTGGAACTGGGCTTTGTAGTGTTCCCCCGCCTTCCACCCTCCTCCGGAGCGGGGGAGGGAGCGGGAGCTGGCA CAGATAGGAGAGAAAGCAGGAGCGGATGTGGAGGGCTTTGGTGCTGAACTTG |
| mm4_exon_Ndc1_chr4_107415950 |
CCTGGAAGGTGAGGGGTCATTCTTTTTCTCCATTGTATATTGGTGGGCACTCTTTTTTTTTAAAGGCACCTTTCCTCCCG CATGCAACGAGGACTTGAGGGAGGCCAGCAGGAGAGAAGCGTTGGCTTTGGTGAAGTT |
| mm4_intergenic_1500009L16Rik|Nuak1_chr10_84029650 |
GCCTTCTGAGCTGTGTGGATCAGAAGACAAAAGCCAGATGATAACATGCATACAGCAAAGAAGGGCTTTCAATCCCAAGC AGGATGAGGGAGGAGGGAAATAACAAGGTATCTACAGCTGTGTGAAGCCTCG |
| mm4_exon_Taf10_chr7_105744052 |
CGGCACCTAGTCCGAGATGCCCTCCTCCCTCACCCGGCCCGGCCCTCCCTCACCTGGCCCGCGCCGCTCTGCCGGCTCCC CTGCCCGTGCCGCCACGGGGCCCGTGCCCGCAGTGGCGACTCCAGCCA |
| mm4_intergenic_Ppp1r15b|Plekha6_chr1_133192455 |
CTCTCTTATGGCTGCTCGGGATGCTCAGCTGACCACCTCCTGGTGCCCAGGCCGCATGCTGGAGGAAGCAGGTGGCAAAT TCTGTGTGTGAGGCTCACATGTTCAATTCATGTGGGGCTGGG |
| mm4_intron_Gm15261_chrX_169101930 |
TGGAGGTTGAGTGGGAATTGGGACTGTTTTTGGACATTCAGAGACCAAGGATCTACCCTTCCTCCCCCATGCGCCATGGC TTGGAACAAGGAGGAATAATACAGAGATGATACCTCCAATGGCAGATTTGGCTCAGGAC |
| mm4_intergenic_AU022754|Mirlet7c-2_chr15_85656262 |
GCCAGGCTCCCAACTTGTAAGATCCCACCCGAATTGGAAAGAAGGTGTCCTCCAAGGATGGGGGAGGAAGGCAGTCAGAG CTGTGAAGGCTGT |
| mm4_exon_Crebzf_chr7_90442862 |
GAGAGTAACTTCCGGCCAGGCCGCTGTCTGGGTGGCGCGGCCGAGTCCTCCCCCGGGCCTCGCCGCTTCGCGCTTCCACT GCCTTCCCCCCGCCTCGTGCGGGGGGTTGGGCGGCCGCCGGAGGCCACTCGGACCTTG |
| mm4_intron_Usp12_chr5_146754760 |
GGAGAACAGACAACTACGGCATCTGAACTTCTGACTGATGCAGAGGGAAAGAAGGTAAGGGGAATGATGGCTCCATGCCG GCTGGGGGAGGCAGGCAGCAGCTGATCTTCACCACC |
| mm4_intron_Arf3_chr15_98762304 |
AACTCAGGGCCAGTGACAAGCTGCTCCCTCCTTCGGTGACACCGCCCCCGCTCCGCCTGGGGGAGGAAGGCCCCACCTCT CTCCTCCGAGCTAGCTC |
| mm4_exon_Hipk3_chr2_104493370 |
TAACGAGAGAAGCCGGCAACAGCCCCGCCGCGCGGCCGCCACGCCTTCCTCCCGCCGCCGCTGGGGACGCGAAGCCTCGG ATCACATTTCTCCTCCCCAACAATCACACGCACGCACGTCTCCAGAGTTGACA |
| mm4_exon_Ctif_chr18_75432159 |
TCATCAGAGGCAGGCTTTGATAGGCCGTTCCTCCCGCAGCAGGAGAGGCTCTGTCCCGGGGAGTCAGGGTCTGTGGGTGC CTGCAAGTCCCCTACTTCCTGCTTGAGTTATCCCCCACAGGAAGGTGCTCAGCCTTT |