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Contents:

PCR primers for off-targets of TGCCGCCTTCCTCCCGCATC CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
mm0_exon_Pop5_chr5_115238016_F TCGTCGGCAGCGTCGAGCGTGGAGATTGACAGGG 60.4 1.00
mm0_exon_Pop5_chr5_115238016_R GTCTCGTGGGCTCGGTTACCTGTGCTTGAACCGCA 60.1 1.00
mm3_intron_Rhd_chr4_134870564_F Primer3: not found at this Tm N.d. 0.73
mm3_intron_Rhd_chr4_134870564_R Primer3: not found at this Tm N.d. 0.73
mm4_intron_Loxhd1_chr18_77372676_F Primer3: not found at this Tm N.d. 0.56
mm4_intron_Loxhd1_chr18_77372676_R Primer3: not found at this Tm N.d. 0.56
mm4_intergenic_Diap2|Gm14984_chrX_130389024_F TCGTCGGCAGCGTCGCAACCCTACCTGTGCTCTC 60.3 0.50
mm4_intergenic_Diap2|Gm14984_chrX_130389024_R GTCTCGTGGGCTCGGGTTCACACAACTAAGCCATGGT 59.1 0.50
mm4_exon_B4galnt1_chr10_127165403_F TCGTCGGCAGCGTCGTCTACAAAGCCCCGGAGTC 60.1 0.25
mm4_exon_B4galnt1_chr10_127165403_R GTCTCGTGGGCTCGGCCTCATGAGGCCGGCGAC 62.8 0.25
mm4_exon_Brix1_chr15_10475663_F Primer3: not found at this Tm N.d. 0.24
mm4_exon_Brix1_chr15_10475663_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Zfp217|Gm17619_chr2_170187516_F Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Zfp217|Gm17619_chr2_170187516_R Primer3: not found at this Tm N.d. 0.24
mm4_exon_Neat1/NEAT1_3/NEAT1_2/NEAT1_1_chr19_5839836_F TCGTCGGCAGCGTCGGAAACCAACAGCTGAAGGC 59.6 0.19
mm4_exon_Neat1/NEAT1_3/NEAT1_2/NEAT1_1_chr19_5839836_R GTCTCGTGGGCTCGGTGCTCTACTGCATTACTGTCTGA 59.2 0.19
mm4_intron_Pdia5_chr16_35405925_F TCGTCGGCAGCGTCCCACGCACGGCTTTCTAAAA 59.4 0.17
mm4_intron_Pdia5_chr16_35405925_R GTCTCGTGGGCTCGGGTCTTCCATCCCACCCACAG 60.0 0.17
mm4_intron_Map3k2_chr18_32187736_F TCGTCGGCAGCGTCTAGAAGCTGTGTGTGGCCAA 59.5 0.16
mm4_intron_Map3k2_chr18_32187736_R GTCTCGTGGGCTCGGCTGATTCCCTGTCAGCTGCA 60.0 0.16
mm3_intergenic_Pou4f2|Rbmxl1_chr8_78480669_F Primer3: not found at this Tm N.d. 0.14
mm3_intergenic_Pou4f2|Rbmxl1_chr8_78480669_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_Vwf_chr6_125556161_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Vwf_chr6_125556161_R Primer3: not found at this Tm N.d. 0.13
mm4_exon_Gstcd_chr3_133082112_F TCGTCGGCAGCGTCGGGCAAAAGCACGATGTCTG 60.1 0.13
mm4_exon_Gstcd_chr3_133082112_R GTCTCGTGGGCTCGGGGGAGCCTTCTCACATCAGG 59.8 0.13
mm4_intergenic_Gm3839|Ptprg_chr14_11552734_F TCGTCGGCAGCGTCTTTGCCCTCTGCCTAACCAG 59.9 0.12
mm4_intergenic_Gm3839|Ptprg_chr14_11552734_R GTCTCGTGGGCTCGGAAGGTCTGTCGCGGAGTTTT 59.8 0.12
mm3_intergenic_Ifrd1|Zfp277_chr12_40281730_F Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_Ifrd1|Zfp277_chr12_40281730_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Adcyap1r1|Neurod6_chr6_55620707_F TCGTCGGCAGCGTCTCATGAAGAGCAAGCTCAGGT 59.3 0.12
mm4_intergenic_Adcyap1r1|Neurod6_chr6_55620707_R GTCTCGTGGGCTCGGAGGGATCTTGAAGCTTCCAGC 60.0 0.12
mm4_intergenic_Gm25514|Gm13981_chr2_117011374_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm25514|Gm13981_chr2_117011374_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Zc3h18_chr8_122412071_F TCGTCGGCAGCGTCCCCTGAAGCAGTTGGGAGAA 59.5 0.11
mm4_intron_Zc3h18_chr8_122412071_R GTCTCGTGGGCTCGGGGCCTTGGACAAAAGCTAAGG 59.4 0.11
mm3_intergenic_Gm9884|Gm24064_chr1_25971716_F Primer3: not found at this Tm N.d. 0.11
mm3_intergenic_Gm9884|Gm24064_chr1_25971716_R Primer3: not found at this Tm N.d. 0.11
mm3_intron_Fggy_chr4_95920985_F Primer3: not found at this Tm N.d. 0.11
mm3_intron_Fggy_chr4_95920985_R Primer3: not found at this Tm N.d. 0.11
mm3_intergenic_Sec11a|Zfp592_chr7_80993454_F Primer3: not found at this Tm N.d. 0.11
mm3_intergenic_Sec11a|Zfp592_chr7_80993454_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Stim2|Gm24235_chr5_54424806_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Stim2|Gm24235_chr5_54424806_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm25442|Gm25696_chr3_35511080_F TCGTCGGCAGCGTCCCCAAAAGCTCCACCCTGAT 59.9 0.10
mm4_intergenic_Gm25442|Gm25696_chr3_35511080_R GTCTCGTGGGCTCGGACTTCCCTAGACCTGGGCTT 59.8 0.10
mm4_intergenic_Anks1|Gm15597_chr17_27927639_F TCGTCGGCAGCGTCTGAGACCCTTTGCCATGTGG 60.2 0.10
mm4_intergenic_Anks1|Gm15597_chr17_27927639_R GTCTCGTGGGCTCGGTTTCACAGGAAGCGCTGACT 59.8 0.10
mm4_exon_Rab11fip1_chr8_27154317_F Primer3: not found at this Tm N.d. 0.10
mm4_exon_Rab11fip1_chr8_27154317_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Col6a6_chr9_105748205_F TCGTCGGCAGCGTCCGGAGGAGAAAGGAGCTTGG 60.1 0.10
mm4_intron_Col6a6_chr9_105748205_R GTCTCGTGGGCTCGGGCTCTCTTGCTAACTCCCTGT 59.4 0.10
mm4_intergenic_Gm15899|Gm10612_chr8_122688093_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm15899|Gm10612_chr8_122688093_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Dicer1|Clmn_chr12_104756530_F TCGTCGGCAGCGTCTTTCTGCCAGAGAGAGACGC 59.7 0.08
mm4_intergenic_Dicer1|Clmn_chr12_104756530_R GTCTCGTGGGCTCGGTGGCTCAGTCCTGTCTGGTA 59.8 0.08
mm4_exon_Nphs2_chr1_156310901_F Primer3: not found at this Tm N.d. 0.07
mm4_exon_Nphs2_chr1_156310901_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Hmx1|Cpz_chr5_35413387_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Hmx1|Cpz_chr5_35413387_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Akap7_chr10_25181450_F TCGTCGGCAGCGTCAAATGGGTGAGTCTAGGGTAAAA 57.1 0.06
mm4_intron_Akap7_chr10_25181450_R GTCTCGTGGGCTCGGGCTAGTCTACCTGGTCCACC 58.8 0.06
mm4_intergenic_Grm2|Tex264_chr9_106657050_F TCGTCGGCAGCGTCAGAGGGAGGACAGCTCTGAG 60.0 0.06
mm4_intergenic_Grm2|Tex264_chr9_106657050_R GTCTCGTGGGCTCGGCAGCACAGCCTGACACTACA 59.9 0.06
mm4_exon_Erc1_chr6_119722213_F TCGTCGGCAGCGTCTGACCTGCAGTTGCTGAGAG 59.9 0.05
mm4_exon_Erc1_chr6_119722213_R GTCTCGTGGGCTCGGACACAAGGAACAGGTGGAGA 58.4 0.05
mm4_exon_Cidec_chr6_113428492_F TCGTCGGCAGCGTCTGGTATCTTTTGCCAGGGCT 59.2 0.04
mm4_exon_Cidec_chr6_113428492_R GTCTCGTGGGCTCGGACCTTCTTCCAGGTCCAGGA 59.8 0.04
mm4_intron_Rad54l2_chr9_106696440_F TCGTCGGCAGCGTCTTCAGCACCCTCTGTTGCTC 60.2 0.04
mm4_intron_Rad54l2_chr9_106696440_R GTCTCGTGGGCTCGGGCATGTGTGGATATTTTGTCTGGA 59.6 0.04
mm4_intergenic_Scg3|Gm3671_chr9_75684063_F TCGTCGGCAGCGTCGAGGAAATGAGGAGGAGGCG 59.8 0.04
mm4_intergenic_Scg3|Gm3671_chr9_75684063_R GTCTCGTGGGCTCGGCGGCAAGGAAAATACGCGAG 59.9 0.04
mm4_intergenic_Mki67|Gm9341_chr7_135818027_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Mki67|Gm9341_chr7_135818027_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm7481|Prkd1_chr12_50335943_F TCGTCGGCAGCGTCATGTAGGCTGGGGAAATGGC 60.1 0.04
mm4_intergenic_Gm7481|Prkd1_chr12_50335943_R GTCTCGTGGGCTCGGAAGTACTGCGGCCGTAGAAG 59.8 0.04
mm4_intron_Cubn_chr2_13485107_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Cubn_chr2_13485107_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ptprn2_chr12_116486722_F TCGTCGGCAGCGTCGTGGATGGTAGGCAGTGAGG 59.8 0.04
mm4_intron_Ptprn2_chr12_116486722_R GTCTCGTGGGCTCGGCCTCCTCTCTGCAGTCCCTT 60.6 0.04
mm4_exon_Prdm12_chr2_31642380_F TCGTCGGCAGCGTCAAGACGTGGATCAAGGCAGG 60.0 0.03
mm4_exon_Prdm12_chr2_31642380_R GTCTCGTGGGCTCGGCCTGCCAACTGGAATCCCTT 59.9 0.03
mm4_intergenic_4933434M16Rik|2610035D17Rik_chr11_112941982_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_4933434M16Rik|2610035D17Rik_chr11_112941982_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Fndc5_chr4_129141942_F TCGTCGGCAGCGTCCAATGATAGGGGTGGCTGGG 60.1 0.03
mm4_intron_Fndc5_chr4_129141942_R GTCTCGTGGGCTCGGTGCCTGCCAGATCCCTTCTA 60.3 0.03
mm3_intergenic_Gm20424|Lbx1_chr19_45165088_F TCGTCGGCAGCGTCGGGGTGAGAAATGGGCTCTC 60.1 0.03
mm3_intergenic_Gm20424|Lbx1_chr19_45165088_R GTCTCGTGGGCTCGGTCTCCTCTCACAAGAACCAGC 59.3 0.03
mm4_intron_Ppard_chr17_28290153_F TCGTCGGCAGCGTCCTTTTGCCCCAGAGTCCCTT 59.8 0.02
mm4_intron_Ppard_chr17_28290153_R GTCTCGTGGGCTCGGGGGTGGCAGGGAAGTTTAGT 59.5 0.02
mm4_exon_Chd8/Gm26590_chr14_52205600_F TCGTCGGCAGCGTCAACACAGACCCGTTTACCCT 58.8 0.02
mm4_exon_Chd8/Gm26590_chr14_52205600_R GTCTCGTGGGCTCGGGGTTTTGTGGCCATGTGTCG 60.3 0.02
mm4_intergenic_Gm6984|Htr2a_chr14_74026107_F TCGTCGGCAGCGTCCAGGCTGAAGGAACTCAGGG 60.0 0.02
mm4_intergenic_Gm6984|Htr2a_chr14_74026107_R GTCTCGTGGGCTCGGATCTGCTGCATTTCTCGGGT 59.7 0.02
mm4_exon_Prss48_chr3_85997276_F TCGTCGGCAGCGTCATCCTTCCTCGACTGCCTCT 60.0 0.02
mm4_exon_Prss48_chr3_85997276_R GTCTCGTGGGCTCGGAGTGAGGCGTGTGAACAACT 59.8 0.02
mm4_intron_Rab3c_chr13_110062323_F TCGTCGGCAGCGTCCACCAGCCAGTCCTCTCATC 59.8 0.01
mm4_intron_Rab3c_chr13_110062323_R GTCTCGTGGGCTCGGTCCACAGCATCTAGTTGGGC 59.7 0.01
mm4_intron_Grm7_chr6_111500185_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Grm7_chr6_111500185_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm17396|Rbms3_chr9_117340649_F TCGTCGGCAGCGTCTACATCCATCCAAGCCAGGC 59.8 0.01
mm4_intergenic_Gm17396|Rbms3_chr9_117340649_R GTCTCGTGGGCTCGGAGTCCTGGCATTTCATCCCC 59.7 0.01
mm3_intron_Zbtb7c_chr18_75962032_F Primer3: not found at this Tm N.d. 0.01
mm3_intron_Zbtb7c_chr18_75962032_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Thap8/Wdr62_chr7_30280090_F TCGTCGGCAGCGTCCCGCCTGAGGGAGCAAATAA 60.1 0.01
mm4_exon_Thap8/Wdr62_chr7_30280090_R GTCTCGTGGGCTCGGAGGCTGGTCGTCCTGTTTAC 59.6 0.01
mm4_intron_Hnrnph3_chr10_63023206_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Hnrnph3_chr10_63023206_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Ppp1r16a_chr15_76693267_F TCGTCGGCAGCGTCGGGTTCAGTGAGGTGGCTAC 60.0 0.01
mm4_exon_Ppp1r16a_chr15_76693267_R GTCTCGTGGGCTCGGCCATCATGGTCCTTAGCGCT 59.8 0.01
mm4_intergenic_Sgcz|Gm16204_chr8_38024886_F TCGTCGGCAGCGTCTCCCAATACAGATTCCTGATGAGA 58.7 0.01
mm4_intergenic_Sgcz|Gm16204_chr8_38024886_R GTCTCGTGGGCTCGGCTCAAAGGACCTGGGCTCAG 60.0 0.01
mm4_intergenic_Ajap1|Gm25880_chr4_153579661_F TCGTCGGCAGCGTCGAACAGACTGGCTTCTGGCT 59.9 0.01
mm4_intergenic_Ajap1|Gm25880_chr4_153579661_R GTCTCGTGGGCTCGGCCGGAGAAGAAACCAAGCCT 59.9 0.01
mm4_exon_Il1rapl2_chrX_137571183_F TCGTCGGCAGCGTCTGGTAGCACCGCCAACTG 59.9 0.00
mm4_exon_Il1rapl2_chrX_137571183_R GTCTCGTGGGCTCGGGCCCGCCTCCAAGATTCTTT 60.6 0.00
mm4_intergenic_Gm15958|Pmaip1_chr18_66455125_F TCGTCGGCAGCGTCAGTGAGAGATACCGTTACAAGAAGA 59.0 0.00
mm4_intergenic_Gm15958|Pmaip1_chr18_66455125_R GTCTCGTGGGCTCGGGCCCAATAGCTATGACACTCCA 59.8 0.00
mm4_intron_Sp6_chr11_97018060_F TCGTCGGCAGCGTCCCAACCCTCTGATCCCAGTG 59.7 0.00
mm4_intron_Sp6_chr11_97018060_R GTCTCGTGGGCTCGGGTCTTCCTCAGTTTCCGGCA 59.9 0.00
mm4_intergenic_n-R5s92|Gadl1_chr9_115835219_F TCGTCGGCAGCGTCATCAATGCTGGAGGAGAGGC 59.5 0.00
mm4_intergenic_n-R5s92|Gadl1_chr9_115835219_R GTCTCGTGGGCTCGGTGGATGTGTCTCTGGGCTCT 60.2 0.00
mm4_exon_Fam207a/2810425M01Rik_chr10_77488360_F TCGTCGGCAGCGTCGCCACGTGTGAGTCCTCTAG 59.8 0.00
mm4_exon_Fam207a/2810425M01Rik_chr10_77488360_R GTCTCGTGGGCTCGGCAACCTACAGAGCCAGTCCC 59.7 0.00
mm4_intron_Csmd2_chr4_128406387_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Csmd2_chr4_128406387_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fam20a_chr11_109713791_F TCGTCGGCAGCGTCGGAGCTGAGCTGTGAGAACA 59.6 0.00
mm4_intron_Fam20a_chr11_109713791_R GTCTCGTGGGCTCGGGGTGTCTCCGTTTCAGAGCA 59.9 0.00
mm4_exon_Amica1_chr9_45101082_F TCGTCGGCAGCGTCAGCTGGTGATCATTGTGGGG 60.0 0.00
mm4_exon_Amica1_chr9_45101082_R GTCTCGTGGGCTCGGGTGTACACACTCAGCCTCCT 59.0 0.00
mm4_intergenic_Usp12|Rpl21_chr5_146795143_F TCGTCGGCAGCGTCGAGGACGGCTCCACCCAG 61.7 0.00
mm4_intergenic_Usp12|Rpl21_chr5_146795143_R GTCTCGTGGGCTCGGATCCCCTAAAGACCCGCTCT 60.0 0.00
mm4_intron_Wdr19_chr5_65231986_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Wdr19_chr5_65231986_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_4931406P16Rik_chr7_34261861_F TCGTCGGCAGCGTCGTGTGGGATCTGGCTGATGA 59.4 0.00
mm4_intron_4931406P16Rik_chr7_34261861_R GTCTCGTGGGCTCGGTGCCATAGGAGAAGAGGCTG 58.8 0.00
mm4_intron_Usp12_chr5_146754765_F TCGTCGGCAGCGTCAGGGGAATGATGGCTCCATG 59.5 0.00
mm4_intron_Usp12_chr5_146754765_R GTCTCGTGGGCTCGGGCCTCTACTGAAGCGGTTGT 60.0 0.00
mm4_intron_Rb1_chr14_73295640_F TCGTCGGCAGCGTCTCCAACAAGGACAGGCTGAC 59.8 0.00
mm4_intron_Rb1_chr14_73295640_R GTCTCGTGGGCTCGGGCACAAGCTGTCTGGAATGC 60.1 0.00
mm4_intron_Pebp4_chr14_70036184_F TCGTCGGCAGCGTCTTTCTAGCTGGCGCCTGAAA 59.9 0.00
mm4_intron_Pebp4_chr14_70036184_R GTCTCGTGGGCTCGGTTCCAGGGAGCTTGCATCTG 60.0 0.00
mm4_intron_Pdzrn4_chr15_92447983_F TCGTCGGCAGCGTCTCTTACAGGCGCTTGCTGAA 59.9 0.00
mm4_intron_Pdzrn4_chr15_92447983_R GTCTCGTGGGCTCGGGAGCCAAAAGGTGTTCGCAA 59.6 0.00
mm4_intron_Lmf1_chr17_25605782_F TCGTCGGCAGCGTCCTCACAGAACACCGCTTCCT 59.9 0.00
mm4_intron_Lmf1_chr17_25605782_R GTCTCGTGGGCTCGGAGTCTGCAACTGTGATGGGG 59.9 0.00
mm4_intron_Fam189a1_chr7_65035383_F TCGTCGGCAGCGTCGGATGAGCAGTAGGTACCACC 59.5 0.00
mm4_intron_Fam189a1_chr7_65035383_R GTCTCGTGGGCTCGGTCATATGGTGATCCTGCCGG 59.3 0.00
mm4_intron_Elp5_chr11_69968844_F TCGTCGGCAGCGTCGCTCCTGAGAACAAGCCCTT 59.9 0.00
mm4_intron_Elp5_chr11_69968844_R GTCTCGTGGGCTCGGCTCTCCAGTAAAGCCACGGG 60.1 0.00
mm4_intron_Casz1_chr4_148931872_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Casz1_chr4_148931872_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Abcc1_chr16_14447277_F TCGTCGGCAGCGTCCCGCAGCCCTTCTTTTACTT 58.4 0.00
mm4_intron_Abcc1_chr16_14447277_R GTCTCGTGGGCTCGGCTCAGGGGAGGGAAGTAGAC 58.2 0.00
mm4_intergenic_Tmem45b|Barx2_chr9_31608045_F TCGTCGGCAGCGTCGCCTCGAGACCTCTGCTTTT 60.0 0.00
mm4_intergenic_Tmem45b|Barx2_chr9_31608045_R GTCTCGTGGGCTCGGCTCCTTCTGGCCTGAAGCTC 60.1 0.00
mm4_intergenic_Mesp2|Anpep_chr7_79821291_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Mesp2|Anpep_chr7_79821291_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Loh12cr1|Dusp16_chr6_134711829_F TCGTCGGCAGCGTCACTTTCTGGGCAGGCTCTTG 60.2 0.00
mm4_intergenic_Loh12cr1|Dusp16_chr6_134711829_R GTCTCGTGGGCTCGGGAGATGATGGCCACTCCCAG 59.8 0.00
mm4_intergenic_Gpd1l|Osbpl10_chr9_114945328_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gpd1l|Osbpl10_chr9_114945328_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gpc6|Dct_chr14_117988435_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gpc6|Dct_chr14_117988435_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm12939|Sfpq_chr4_127005045_F TCGTCGGCAGCGTCGAGGCCACTTGCTTCTGGTC 60.9 0.00
mm4_intergenic_Gm12939|Sfpq_chr4_127005045_R GTCTCGTGGGCTCGGTCCTGAAACTCTGAGCAGCG 60.0 0.00
mm4_intergenic_Gm11824|1700120G11Rik_chr4_14190644_F TCGTCGGCAGCGTCGGCAGGATGTATGGAAGCCA 59.8 0.00
mm4_intergenic_Gm11824|1700120G11Rik_chr4_14190644_R GTCTCGTGGGCTCGGACCTGGTCCTCGTCAATGTG 59.6 0.00
mm4_intergenic_Gjb5|Gm12947_chr4_127404267_F TCGTCGGCAGCGTCAGCTAGAGATTGGGAGAGGCA 60.0 0.00
mm4_intergenic_Gjb5|Gm12947_chr4_127404267_R GTCTCGTGGGCTCGGATGGTTTCTGGCCAGCTCAG 60.3 0.00
mm4_intergenic_Cox8b|Nlrp6_chr7_140904233_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cox8b|Nlrp6_chr7_140904233_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Clec16a|Gm15558_chr16_10654096_F TCGTCGGCAGCGTCAGTGATGCTCTCTGGGGCTA 60.0 0.00
mm4_intergenic_Clec16a|Gm15558_chr16_10654096_R GTCTCGTGGGCTCGGTTCCTGAGGCAAGGGGTAGT 60.1 0.00
mm4_intergenic_Cacna2d3|Selk_chr14_29830079_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cacna2d3|Selk_chr14_29830079_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_BC034090|Xpr1_chr1_155237553_F TCGTCGGCAGCGTCCACCCCACCTCAGATGAAGC 60.3 0.00
mm4_intergenic_BC034090|Xpr1_chr1_155237553_R GTCTCGTGGGCTCGGTAAGGCAGGCAGGAAAGAGC 60.0 0.00
mm4_intergenic_5730508B09Rik|Gm10650_chr3_127929397_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_5730508B09Rik|Gm10650_chr3_127929397_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_2810011L19Rik|D430019H16Rik_chr12_105448723_F TCGTCGGCAGCGTCAAAAGACCAGGCCTCCACCT 61.0 0.00
mm4_intergenic_2810011L19Rik|D430019H16Rik_chr12_105448723_R GTCTCGTGGGCTCGGGACCTCTGGCACTCATCTGG 59.8 0.00
mm4_exon_Tln1_chr4_43549406_F TCGTCGGCAGCGTCCAAGGCCCTTTGCTGCTTG 60.0 0.00
mm4_exon_Tln1_chr4_43549406_R GTCTCGTGGGCTCGGTGTGCAGTCACCACCATCTC 59.9 0.00
mm4_exon_Rest_chr5_77268098_F TCGTCGGCAGCGTCGCCCCTCAGCTCATCATGTT 60.1 0.00
mm4_exon_Rest_chr5_77268098_R GTCTCGTGGGCTCGGTCTCCCACGGGCATCAATTC 60.1 0.00
mm4_exon_Plxnd1_chr6_115994190_F TCGTCGGCAGCGTCAGCGGTGGTCTTCTAGGCTA 60.0 0.00
mm4_exon_Plxnd1_chr6_115994190_R GTCTCGTGGGCTCGGGTCCACTGTGGGACTGGTG 59.9 0.00
mm3_intergenic_Large|Gm23255_chr8_73604805_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Large|Gm23255_chr8_73604805_R Primer3: not found at this Tm N.d. 0.00
mm2_exon_Antxr2_chr5_97885216_F TCGTCGGCAGCGTCGAGCTATTTCCTTCGGCTGC 59.0 0.00
mm2_exon_Antxr2_chr5_97885216_R GTCTCGTGGGCTCGGTCACCATCGTGTCACGTCAA 59.6 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm0_exon_Pop5_chr5_115238016 GAGCGTGGAGATTGACAGGGCACGCTGCCTGTCCGAGCCTAGTGCCGCCTTCCTCCCGCATCCGGCGCGGACGCCATGGT
GCGGTTCAAGCACAGGTAA
mm4_intergenic_Diap2|Gm14984_chrX_130389024 GCAACCCTACCTGTGCTCTCTATTGAATTGCACTTCCTGCTTCTGCCACCATCCTCTCACATCCGGGGCTACTACCTCTG
AAATCTTTAGATTATAAAGTCACAATCATCTAAAACCATGGCTTAGTTGTGTGAAC
mm4_exon_B4galnt1_chr10_127165403 GTCTACAAAGCCCCGGAGTCCGCAGGCGGGAGGAGTCGGATCCCCGACACTGTCGGGCTCCCTCCCGCCCAGCTCCCGCA
TCAGG
CCGTCGCCGGCCTCATGAGG
mm4_exon_Neat1/NEAT1_3/NEAT1_2/NEAT1_1_chr19_5839836 GGAAACCAACAGCTGAAGGCGGCTGGCACGCCTGCCCAGCAGTGGACAGCACCTGCTGCGGGAGGAAGTCGTCCTCTCCC
TCCACACGAGTTCGTGTGTAAATAGGATTGTTTAATCAGACAGTAATGCAGTAGAGCA
mm4_intron_Pdia5_chr16_35405925 CCACGCACGGCTTTCTAAAATGGGAGGTGAATGTCTAAGAAGCAGGAAACCATTAGGCCTCCTGATGTGGGAGGACAGAG
GCA
AAGCCTGTGGGTGGGATGGAAGAC
mm4_intron_Map3k2_chr18_32187736 TAGAAGCTGTGTGTGGCCAAGACCACCACATAAAGGAGTGTGTTTACTCTCTGAAGGTTGTAGCCTAATCATACAGCCTG
TCCTGAGGTGGGAGGAGGGTGGCAATGCAGCTGACAGGGAATCAG
mm4_exon_Gstcd_chr3_133082112 GGGCAAAAGCACGATGTCTGCCAAAGTGAAGTACAGTCCTTCTGCAAACACGTGCTCCAGAGGCGGGAGGTCGGCGGCTT
TTGCTTTCCTGATGTGAGAAGGCTCCC
mm4_intergenic_Gm3839|Ptprg_chr14_11552734 TTTGCCCTCTGCCTAACCAGGCCAAGTTGCAGCCACCTTCTTCCCGAATCCGGGGGTTGGGAAAACTCCGCGACAGACCT
T
mm4_intergenic_Adcyap1r1|Neurod6_chr6_55620707 TCATGAAGAGCAAGCTCAGGTTTGAAATCCAGCTGCCTGCCTCCCGCAGCAGGGCTTCCCCAGTCAATTTCTGCTGGAAG
CTTCAAGATCCCT
mm4_intron_Zc3h18_chr8_122412071 CCCTGAAGCAGTTGGGAGAAAGATCCCTTAAAGAAAACCAAAAAGGAGTTAGAAGGTAGAGTCAGCTTGATGCTTTTGGA
ATCTGGTGACCATTTTTCCTCTGTCCTCCCGCATCAGGGCCTTAGCTTTTGTCCAAGGCC
mm4_intergenic_Gm25442|Gm25696_chr3_35511080 CCCAAAAGCTCCACCCTGATTGGCCTTAGTGCTGCCTCCCTCCCGAATTTGGTTAAGCCCAGGTCTAGGGAAGT
mm4_intergenic_Anks1|Gm15597_chr17_27927639 TGAGACCCTTTGCCATGTGGGAGTTAGGCTAGGCCGGCCCGCCTTCCTTCAGCATCAGGACTTGACATAGTTATTTGTTC
ATTGAGACGTGCAAAAGCCCAGTCAGCGCTTCCTGTGAAA
mm4_intron_Col6a6_chr9_105748205 CGGAGGAGAAAGGAGCTTGGCTGACTCCTTCCTACCACATCAGGCTTCTTCCCTACAGGGAGTTAGCAAGAGAGC
mm4_intergenic_Dicer1|Clmn_chr12_104756530 TTTCTGCCAGAGAGAGACGCTGGTGCCAAACACGTAGAGATTTTTGTCTAGCTGTTACACTGGAAAAAAAAGAACATATC
ACTTCAGTAGGATGGCGTCTTCCACCAGCATCAGGGTACCAGACAGGACTGAGCCA
mm4_intron_Akap7_chr10_25181450 AAATGGGTGAGTCTAGGGTAAAAAACCCAAAGCCTGGTGTGGGTGGAAGGAGGCAGTGCTCACGTCAGATTGTAGACTGA
GTCAGTATTCCCAGGTGGACCAGGTAGACTAGC
mm4_intergenic_Grm2|Tex264_chr9_106657050 AGAGGGAGGACAGCTCTGAGAAGCTGCTTGACTCCTTCCTCCCACAGCAGGATGGGGAAGGACACCCCTGGGTAGGGGGT
GGTGGATAAGCTTTGTAGTGTCAGGCTGTGCTG
mm4_exon_Erc1_chr6_119722213 TGACCTGCAGTTGCTGAGAGCTGTCGCTGAGACTGTCCTCCCTTCTGCGGGCCTCCTCCAGCATCTGGGCACTCTTCTTC
TTCTCCACCTGTTCCTTGTGT
mm4_exon_Cidec_chr6_113428492 TGGTATCTTTTGCCAGGGCTTGGAAGTATTCTTCTGTCTCCACGATTGTGCCATCTTCCTCCAGCACCAGGGAGAAGGGC
TTGTCTTTAAGTTTCAAGATGTCCTGGACCTGGAAGAAGGT
mm4_intron_Rad54l2_chr9_106696440 TTCAGCACCCTCTGTTGCTCAGCGGGGAGAATCCTGGAGCTGTACTCTGTCCACCAGTTCTCTTCCAGGAAGCCTGATGC
CTTCTTCCAGCATCAGG
CACATATGTCCAGACAAAATATCCACACATGC
mm4_intergenic_Scg3|Gm3671_chr9_75684063 GAGGAAATGAGGAGGAGGCGCATGCTCCATGCAAACCAGAGGCGTGAGGATGGCGTCAGCGACCCAGCGCTACTCCAATC
CCGCGGGAACGGAGCGGGGGAGGGGAGACAGGCTTGCTCGCGTATTTTCCTTGCCG
mm4_intergenic_Gm7481|Prkd1_chr12_50335943 ATGTAGGCTGGGGAAATGGCTCTGTCCTGATGCAGGGGGATGGCTGCAAACAACCATGGAGAATTAATACTATAGTCCTT
CTACGGCCGCAGTACTT
mm4_intron_Ptprn2_chr12_116486722 GTGGATGGTAGGCAGTGAGGGGTGAGCTGCAGCCTTGGCCCGAGCTCCCCTCAGGCTCCACACCTCCTTCCTCCCGCCTC
TGG
TCTTCAGAGGTGTTTCAACCTTGCAAAACTCAAAGAAAGGGACTGCAGAGAGGAGG
mm4_exon_Prdm12_chr2_31642380 AAGACGTGGATCAAGGCAGGCACGGAGATGGGCCCCTTCACTGGCCGCGTCATTGCCCCAGAGCATGTGGATATCTGCAA
AAACAACAACCTCATGTGGGAGGTAGGCGGGAGCTTTGGAAAGGGATTCCAGTTGGCAGG
mm4_intron_Fndc5_chr4_129141942 CAATGATAGGGGTGGCTGGGGGCCAGGTAGGGGGGAAAAGGAGCCAGAGGCTGGAGGGAGGCTGCAGCAGCAAGGCAGAG
GCCATTCGGGGATGGCAGTGAGCCCTAGAAGGGATCTGGCAGGCA
mm3_intergenic_Gm20424|Lbx1_chr19_45165088 GGGGTGAGAAATGGGCTCTCATTTTGAAGCCTGCCTCCCGCATCTGACTTCTCCATGATTGGGAGAAGGTATCTAAGCTG
GTTCTTGTGAGAGGAGA
mm4_intron_Ppard_chr17_28290153 CTTTTGCCCCAGAGTCCCTTTGGGGATCCCAAAGGAACCCTGAGACGGTAGGAAGGCTGCAGGGCTCCAACCCCTCCATG
TGGGTCCACACTAAACTTCCCTGCCACCC
mm4_exon_Chd8/Gm26590_chr14_52205600 AACACAGACCCGTTTACCCTAGACTCTACCATCTCACCATCACCAGCAGCTGGGGGAAGGCGGCACACTTGTCGACACAT
GGCCACAAAACC
mm4_intergenic_Gm6984|Htr2a_chr14_74026107 CAGGCTGAAGGAACTCAGGGCAGAAGTGCTCATGTGTCTGTGACACAGTCTTCTAAATATAGCTGGGGGAAGCAGATCCC
TTCTCTGTTCCAGAGACGGGAGAAAGGAGGCACTCACCCGAGAAATGCAGCAGAT
mm4_exon_Prss48_chr3_85997276 ATCCTTCCTCGACTGCCTCTCACCAGCACAGAACATGTCTTCCTTGATGACCCGCTCCAGATCCGGGAGGAAGACACCAA
TTGGGTTGTAGAGTTGTTCACACGCCTCACT
mm4_intron_Rab3c_chr13_110062323 CACCAGCCAGTCCTCTCATCCTGACTCCTTGCTCCCGCCTCTGGTGTGCTCATACATCTTTGTTGACTTGGCTGGATTAA
GAGCAAAGCATGGGGCGTGTCTGCGACTGCTTTTCCAGGCCCAACTAGATGCTGTGGA
mm4_intergenic_Gm17396|Rbms3_chr9_117340649 TACATCCATCCAAGCCAGGCACTGAAGAAGTAACCGGATGCTGCAGGAAGGTGACAGTGGCTGGGTGGTACCCAAGGGGG
ATGAAATGCCAGGACT
mm4_exon_Thap8/Wdr62_chr7_30280090 CCGCCTGAGGGAGCAAATAAAGAAGCGATGGGAGGGACCGAGGCCCCGAGGCGGGACCAAGGTGGCAGAGAAGCCCGGAG
TTGGGTAAACAGGACGACCAGCCT
mm4_exon_Ppp1r16a_chr15_76693267 GGGTTCAGTGAGGTGGCTACCCTGCTGCTGGAGCAAGGCGCCAGCCTGAGCGCTAAGGACCATGATGG
mm4_intergenic_Sgcz|Gm16204_chr8_38024886 TCCCAATACAGATTCCTGATGAGAAAAACTAAATAAATTATTAGGAAACATAAGTAGTATTCTTGTCACTATACTCTTCC
TGATCCTGGGGGAAGGGGGCA
ATGCAGAAGGCACAGGCTGCTGAGCCCAGGTCCTTTGAG
mm4_intergenic_Ajap1|Gm25880_chr4_153579661 GAACAGACTGGCTTCTGGCTGACCCACCCTCTTCTGTCTATTTTACCTCTCTGTGCGCACTGTCTCCTTCCTTCTGCATC
AGG
CTTACGTAAGCAGATCAGCTGTCGGGTGCTAAGAGGCTTGGTTTCTTCTCCGG
mm4_exon_Il1rapl2_chrX_137571183 TGGTAGCACCGCCAACTGGGGTGCCCCGCGTCCCGCGTCCCAGCGTGGCGCGCACTGTTCGGCCTGAACCAGGTGCGGCT
GGAAGGAGGCA
GCACCAAAGCTAACTCGCTCCAAAGAATCTTGGAGGCGGGC
mm4_intergenic_Gm15958|Pmaip1_chr18_66455125 AGTGAGAGATACCGTTACAAGAAGAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNN
NNNNNNNNNNACACACAGACACTGGAGTGTCATAGCTATTGGGC
mm4_intron_Sp6_chr11_97018060 CCAACCCTCTGATCCCAGTGCGCCCCAGCGAGGGTGTCAGGCGCAGGCTGAGCAACCCGGACCGGGAGGAAGGCGGCGGG
TTTAGGAGGTGGATTTCTGTGGTACCCGGCTTGCCGGAAACTGAGGAAGAC
mm4_intergenic_n-R5s92|Gadl1_chr9_115835219 ATCAATGCTGGAGGAGAGGCCTGACTTCTGCCCCCTTCCTCCTGCAGATGGAGAGCCCAGAGACACATCCA
mm4_exon_Fam207a/2810425M01Rik_chr10_77488360 GCCACGTGTGAGTCCTCTAGATCTCATGGCACACACTGTCCTTGGTCAGAGCTGTTTGCCACCTTCCAGCTGCATCTGGT
GTGCCAGCTGCTGCCCGATGGCCAGCAGGGGACTGGCTCTGTAGGTTG
mm4_intron_Fam20a_chr11_109713791 GGAGCTGAGCTGTGAGAACAGATCTGCAGCCTTCCTACAGGATCTGGGACTTTTCTACTCTTAAAGAGAAAAAGGCCGGA
AAGCTACAAATATGCCCATCTCAACTGCTCTGAAACGGAGACACC
mm4_exon_Amica1_chr9_45101082 AGCTGGTGATCATTGTGGGGATCGTCTGTGCCACCTTCCTGCTGCTTCCGGTTTTGATATTAATTGTGAAGAAAGCCAAG
TGGAATAAGAGGTACATGACCGTCCCCCCTCCAGTTAGGAGGCTGAGTGTGTACAC
mm4_intergenic_Usp12|Rpl21_chr5_146795143 GAGGACGGCTCCACCCAGTGCGCGTTGGGGCGGGGCGAGACCCGTGCCGCGTTCCTCCTGCAGATGGGTCCTAGCGCCGA
CGTGAAGCACCTACCTTAGCTTGCAAGAGCGGGTCTTTAGGGGAT
mm4_intron_4931406P16Rik_chr7_34261861 GTGTGGGATCTGGCTGATGAAGAATGCAATGCTACTGGCAACTGCAGCGGCTCTGGAAACTGCCTCCTGCCTCCTGCCTC
AGG
CGGAACACTGATGAGACTATGTAGTTGAGTCTTCTCAGCCTCTTCTCCTATGGCA
mm4_intron_Usp12_chr5_146754765 AGGGGAATGATGGCTCCATGCCGGCTGGGGGAGGCAGGCAGCAGCTGATCTTCACCACCTGGGTCCTTCCACAACCGCTT
CAGTAGAGGC
mm4_intron_Rb1_chr14_73295640 TCCAACAAGGACAGGCTGACTTAACTGCACACTGTCCCCTCCCTCCCCCATCAGGCTGCCCTCCTGTACTATACCTCTCT
ACACCAAGTGCCTGGTCTCTGACACTGCATTCCAGACAGCTTGTGC
mm4_intron_Pebp4_chr14_70036184 TTTCTAGCTGGCGCCTGAAACGGATGGCTTGGAGAGAGGAATGGAGAATGCCTCAGCCAAATGAGGGAGGACGGAGGCAG
ACTATGGGGTCAGAAGCTCTGCAGATGCAAGCTCCCTGGAA
mm4_intron_Pdzrn4_chr15_92447983 TCTTACAGGCGCTTGCTGAAAATAGTACAGGCATGAATGTACCACAGCTCAGAGGCTGGCCGGCACTGTCTTCCCTGGTG
AGGGAGGGAGGGGGCA
GCTTTCATTGCGAACACCTTTTGGCTC
mm4_intron_Lmf1_chr17_25605782 CTCACAGAACACCGCTTCCTTCGTGTGGTCTGTGGCTGCCTGGTGGGGCAGGAAGGCAGCAATGTTGTAGTCCTGGGACC
CAACAGACTCCCCCCCCCCCCGCCCCCCATCACAGTTGCAGACT
mm4_intron_Fam189a1_chr7_65035383 GGATGAGCAGTAGGTACCACCTTGCCCAGATGAGGGAGGAAAGCATCACCGGCAGGATCACCATATGA
mm4_intron_Elp5_chr11_69968844 GCTCCTGAGAACAAGCCCTTGCTCATATACACTGTGGTTTGGTCCCTGCTGCCCACCTCCCCCATCTGGTCTGGCTCTCT
TGGCCCATGGCTACTCAGGGCCCGTGGCTTTACTGGAGAG
mm4_intron_Abcc1_chr16_14447277 CCGCAGCCCTTCTTTTACTTTTCAGTTGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTCTACTTCCCTCCCCTGAG
mm4_intergenic_Tmem45b|Barx2_chr9_31608045 GCCTCGAGACCTCTGCTTTTTCCAGCTGAGGATCTGGAGAAAGATATTGCCTCCCTCCTCCTTCATCTGGTCCTGTCTTA
TTTTTTACAGCACGAGCTTCAGGCCAGAAGGAG
mm4_intergenic_Loh12cr1|Dusp16_chr6_134711829 ACTTTCTGGGCAGGCTCTTGGCCGTGCTGTGCCTCCCTTCTTGGGGGCTGCTTTCCTTCCTGGCGAGGGAGGAAGGCGGT
A
AGGTGCGCTGAGAGCTCTGGGGCACTGCAGCTGGGAGTGGCCATCATCTC
mm4_intergenic_Gm12939|Sfpq_chr4_127005045 GAGGCCACTTGCTTCTGGTCCCTGATGAGGGAGGGAACCGGCAGAGTAGAAACTGTGACCCCAAAGGGATAGGTGATGTG
CTTGTATTCAGTCTGGTTTTACAAAGACGCTGCTCAGAGTTTCAGGA
mm4_intergenic_Gm11824|1700120G11Rik_chr4_14190644 GGCAGGATGTATGGAAGCCATGTGGCAGAAGAATGTGCCAGATGGTAGAGGAAGGTGGCAGAGAAAGGGCAAGTACCCTG
AAGAGATGATGAAGGCAACATCACATTGACGAGGACCAGGT
mm4_intergenic_Gjb5|Gm12947_chr4_127404267 AGCTAGAGATTGGGAGAGGCAGGCAGACAGACACACGGTGCCCCCTTCCTCCCCCAAAAGGATCAGGAGAATCATGACCT
GTTTGGGTTTGCTTGGATTGTATGTACATTTCTAGAGCTGAGCTGGCCAGAAACCAT
mm4_intergenic_Clec16a|Gm15558_chr16_10654096 AGTGATGCTCTCTGGGGCTATGCCCACACAGGACTCTAGACCCTGTGGCTCCCTCTGCCCACTCTGTGGCCTACCTCCCC
CATCTGG
CCCAACAAGAGATGGATCACACAAGGTCTAGTGACTACCCCTTGCCTCAGGAA
mm4_intergenic_BC034090|Xpr1_chr1_155237553 CACCCCACCTCAGATGAAGCATTGCTGCAGCCTCCCTCACCCATCTGGAATGTTCCTTCTGTTTCCACCCAGACCCTTGC
AAGCCCTCACAGGCTCCAGCTGTCGGAGAGCCCTCAGTCAGCTCTTTCCTGCCTGCCTTA
mm4_intergenic_2810011L19Rik|D430019H16Rik_chr12_105448723 AAAAGACCAGGCCTCCACCTGAGGGATCTGCAGCCCCCTGCCTCCCTCATCTGGATCCCAGATGAGTGCCAGAGGTC
mm4_exon_Tln1_chr4_43549406 CAAGGCCCTTTGCTGCTTGCAGAAGGGGCCGGCCATTGCCGCCTTCATCCTCCAGCAGGGCAGCCAATAGCTTCACCCCA
CGGGACATCTCCGTCAGGTTGGAAGAGATGGTGGTGACTGCACA
mm4_exon_Rest_chr5_77268098 GCCCCTCAGCTCATCATGTTAGCCAACGTGGCCCTGACGGGGGAGGCAAGCGGCAGCTGCTGCGATTACCTGGTCGGTGA
AGAGAGGCAGATGGCCGAATTGATGCCCGTGGGAGA
mm4_exon_Plxnd1_chr6_115994190 AGCGGTGGTCTTCTAGGCTACGGTTGCGCGGGAAGAAAGCGCTGCCGAAGCCGGTGTACGTGGCGCCCACGAGCAGACGG
CTGCCCCCGGTGCCCGAGGTAGGCGGCAGCACCAGTCCCACAGTGGAC
mm2_exon_Antxr2_chr5_97885216 GAGCTATTTCCTTCGGCTGCTGCCGCCTTCCTCCCCCACCGGGTTTCTGCTCACACTAACATAATTTGGCATCGTTGACG
TGACACGATGGTGA

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_ozJbN0W08qlgEBn3RvZK.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.