← return to the list of all guides

Contents:

PCR primers for off-targets of CCGCGCCGGATGCGGGAGGA AGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
mm0_exon_Pop5_chr5_115238021_F TCGTCGGCAGCGTCGAGCGTGGAGATTGACAGGG 60.4 1.00
mm0_exon_Pop5_chr5_115238021_R GTCTCGTGGGCTCGGTTACCTGTGCTTGAACCGCA 60.1 1.00
mm4_exon_Usp32_chr11_85139816_F TCGTCGGCAGCGTCGGGTCGGAGCCTGCTTTC 60.4 0.21
mm4_exon_Usp32_chr11_85139816_R GTCTCGTGGGCTCGGGCAGAAGCCGCACTTGTTAG 59.8 0.21
mm4_exon_Cnnm1_chr19_43440428_F TCGTCGGCAGCGTCCTTGCTGCAGTATCACGTGC 59.9 0.21
mm4_exon_Cnnm1_chr19_43440428_R GTCTCGTGGGCTCGGAAGAAGAGCAGGAGTACGGC 59.4 0.21
mm4_intergenic_Cpq|Tspyl5_chr15_33673231_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Cpq|Tspyl5_chr15_33673231_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm22845|Nt5c1b_chr12_10157767_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm22845|Nt5c1b_chr12_10157767_R Primer3: not found at this Tm N.d. 0.20
mm4_exon_Acaca_chr11_84391631_F TCGTCGGCAGCGTCTGCAGTTTGCTGACTTGCAC 59.8 0.20
mm4_exon_Acaca_chr11_84391631_R GTCTCGTGGGCTCGGCAGCTTGTGCGCCTATGTCA 61.0 0.20
mm4_intergenic_Rpl31-ps23|Ppp1r3b_chr8_35249980_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Rpl31-ps23|Ppp1r3b_chr8_35249980_R Primer3: not found at this Tm N.d. 0.19
mm4_exon_Kif19a_chr11_114786361_F Primer3: not found at this Tm N.d. 0.18
mm4_exon_Kif19a_chr11_114786361_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_1500009L16Rik|Nuak1_chr10_84029650_F TCGTCGGCAGCGTCGCCTTCTGAGCTGTGTGGAT 60.0 0.18
mm4_intergenic_1500009L16Rik|Nuak1_chr10_84029650_R GTCTCGTGGGCTCGGCGAGGCTTCACACAGCTGTA 60.0 0.18
mm3_intron_Oca2_chr7_56390704_F TCGTCGGCAGCGTCTATCAGCTCAGCCAGTCAGG 58.8 0.15
mm3_intron_Oca2_chr7_56390704_R GTCTCGTGGGCTCGGCATACCCAGAGAGAGCCCCT 60.1 0.15
mm4_intron_Klf10_chr15_38299563_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Klf10_chr15_38299563_R Primer3: not found at this Tm N.d. 0.14
mm4_exon_BC042782_chr4_95161347_F Primer3: not found at this Tm N.d. 0.13
mm4_exon_BC042782_chr4_95161347_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Ephb3_chr16_21210983_F TCGTCGGCAGCGTCCTCCCTCCCTCTCCAGAAAT 57.8 0.13
mm4_intron_Ephb3_chr16_21210983_R GTCTCGTGGGCTCGGCCTCCACAGTTTAAAACAAGCC 58.0 0.13
mm4_intergenic_Fasl|Suco_chr1_161792570_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Fasl|Suco_chr1_161792570_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm22227|Zfp703_chr8_26830238_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm22227|Zfp703_chr8_26830238_R Primer3: not found at this Tm N.d. 0.11
mm3_exon_Taf10_chr7_105744052_F TCGTCGGCAGCGTCCGGCACCTAGTCCGAGATG 59.6 0.10
mm3_exon_Taf10_chr7_105744052_R GTCTCGTGGGCTCGGTGGCTGGAGTCGCCACTG 62.0 0.10
mm4_intron_Inadl_chr4_98630943_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Inadl_chr4_98630943_R Primer3: not found at this Tm N.d. 0.09
mm4_exon_Olig2_chr16_91227061_F Primer3: not found at this Tm N.d. 0.08
mm4_exon_Olig2_chr16_91227061_R Primer3: not found at this Tm N.d. 0.08
mm4_exon_E330009J07Rik_chr6_40436029_F Primer3: not found at this Tm N.d. 0.08
mm4_exon_E330009J07Rik_chr6_40436029_R Primer3: not found at this Tm N.d. 0.08
mm4_exon_Lphn3_chr5_81020803_F TCGTCGGCAGCGTCTTTGGTCTCCTCAGCAGCTG 59.9 0.08
mm4_exon_Lphn3_chr5_81020803_R GTCTCGTGGGCTCGGACATGCGGCAGTCATAACCA 60.0 0.08
mm4_exon_Wbscr27_chr5_134932652_F TCGTCGGCAGCGTCCTGCCATCTCCCACTCAAGG 60.1 0.07
mm4_exon_Wbscr27_chr5_134932652_R GTCTCGTGGGCTCGGAGTCCACCCAGTGATGAGGT 60.1 0.07
mm4_intergenic_4930529M08Rik|Insm1_chr2_146221517_F TCGTCGGCAGCGTCTGATTTTCCATACGACGCGC 59.3 0.07
mm4_intergenic_4930529M08Rik|Insm1_chr2_146221517_R GTCTCGTGGGCTCGGTGTCAGGAGGGTTCAATCCG 59.3 0.07
mm4_intergenic_Gm25672|Barhl2_chr5_106227956_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm25672|Barhl2_chr5_106227956_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm25784|Vstm2b_chr7_40898618_F TCGTCGGCAGCGTCGAGAGATGGTGGTCGCTACG 59.9 0.06
mm4_intergenic_Gm25784|Vstm2b_chr7_40898618_R GTCTCGTGGGCTCGGGAGCAGCCTCCTCCACAG 59.4 0.06
mm4_exon_Ergic3_chr2_156008077_F Primer3: not found at this Tm N.d. 0.06
mm4_exon_Ergic3_chr2_156008077_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_4932435O22Rik|Gm11689_chr11_114628408_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_4932435O22Rik|Gm11689_chr11_114628408_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Eif4g1_chr16_20672914_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Eif4g1_chr16_20672914_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Dlgap4_chr2_156721065_F TCGTCGGCAGCGTCCCTCTCGGGCGTACAAAACC 61.0 0.05
mm4_exon_Dlgap4_chr2_156721065_R GTCTCGTGGGCTCGGCGGAGTGACGGATGGACTG 59.8 0.05
mm4_intergenic_Gm20109|E230001N04Rik_chr17_28523190_F TCGTCGGCAGCGTCCCCGAGCGAAAGAACATCCT 60.1 0.05
mm4_intergenic_Gm20109|E230001N04Rik_chr17_28523190_R GTCTCGTGGGCTCGGGAAGAAGCAGTACGAAGCGC 59.6 0.05
mm4_exon_Mapk9_chr11_49846937_F Primer3: not found at this Tm N.d. 0.05
mm4_exon_Mapk9_chr11_49846937_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Slc1a3_chr15_8638837_F TCGTCGGCAGCGTCTATGGAGTGGGAGGGAGAGC 60.1 0.04
mm4_intron_Slc1a3_chr15_8638837_R GTCTCGTGGGCTCGGAGTCCTTGCCTAGAGTCCCA 59.5 0.04
mm4_exon_Bcor_chrX_12080181_F TCGTCGGCAGCGTCGAGCTTCAGGGCAGGTGG 60.0 0.04
mm4_exon_Bcor_chrX_12080181_R GTCTCGTGGGCTCGGCTGCTGAGGGTGACAGAGC 60.0 0.04
mm4_intron_Trim24_chr6_37871681_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Trim24_chr6_37871681_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Pcdh10_chr3_45379856_F TCGTCGGCAGCGTCACTGGACCGAGAACAGCAAG 59.9 0.03
mm4_exon_Pcdh10_chr3_45379856_R GTCTCGTGGGCTCGGCAGTACGCTGCTGCTGGG 60.8 0.03
mm4_exon_Zcchc3_chr2_152414838_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_Zcchc3_chr2_152414838_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Lima1|Gm9763_chr15_99875485_F TCGTCGGCAGCGTCGCTGCAGCACCTTCAGCT 60.6 0.03
mm4_intergenic_Lima1|Gm9763_chr15_99875485_R GTCTCGTGGGCTCGGAGCACGTTCGCTCCAAGG 60.3 0.03
mm4_intergenic_Dio3os/Mir1247|Dio3_chr12_110278567_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Dio3os/Mir1247|Dio3_chr12_110278567_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_4930503L19Rik/Stard6_chr18_70472517_F TCGTCGGCAGCGTCCGCTTTCAAACCGACCCAAC 60.3 0.03
mm4_exon_4930503L19Rik/Stard6_chr18_70472517_R GTCTCGTGGGCTCGGCGCCCTTTGACCTCTGAACT 59.9 0.03
mm4_intergenic_1700041M05Rik|Grik3_chr4_125490343_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_1700041M05Rik|Grik3_chr4_125490343_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_Actn1_chr12_80260247_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_Actn1_chr12_80260247_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_Pou3f1_chr4_124660183_F TCGTCGGCAGCGTCCAAGAACCTTGCGCCTGTTT 59.6 0.02
mm4_exon_Pou3f1_chr4_124660183_R GTCTCGTGGGCTCGGGTTCCCACCTCACCCCAAAT 59.8 0.02
mm4_intron_Gm8923_chr2_175470339_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm8923_chr2_175470339_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm8898_chr2_175930750_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm8898_chr2_175930750_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm4723_chr2_175372788_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm4723_chr2_175372788_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm4245_chr2_175703348_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm4245_chr2_175703348_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm14431_chr2_176521330_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm14431_chr2_176521330_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm14419_chr2_177001261_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm14419_chr2_177001261_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm14306_chr2_176670876_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm14306_chr2_176670876_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm8923|Gm11007_chr2_175605799_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm8923|Gm11007_chr2_175605799_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm2004|Gm6182_chr2_176153093_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm2004|Gm6182_chr2_176153093_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gadd45b|Gng7_chr10_80936652_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gadd45b|Gng7_chr10_80936652_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Sema4a|Lmna_chr3_88464929_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Sema4a|Lmna_chr3_88464929_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm11696|Gna13/Gm11696_chr11_109362791_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm11696|Gna13/Gm11696_chr11_109362791_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Ankmy2_chr12_36157856_F TCGTCGGCAGCGTCTAGGCTCCAAGTCGGGTGTA 59.9 0.02
mm4_intron_Ankmy2_chr12_36157856_R GTCTCGTGGGCTCGGAACCCCTGTTGCTGAAAGCT 60.1 0.02
mm4_intergenic_A830035A12Rik/Gm11649|A830035A12Rik/Helz_chr11_107547848_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_A830035A12Rik/Gm11649|A830035A12Rik/Helz_chr11_107547848_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Btbd7|Cox8c_chr12_102878533_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Btbd7|Cox8c_chr12_102878533_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Cnot6l_chr5_96161858_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Cnot6l_chr5_96161858_R Primer3: not found at this Tm N.d. 0.01
mm3_exon_Prss48_chr3_85997271_F TCGTCGGCAGCGTCATCCTTCCTCGACTGCCTCT 60.0 0.01
mm3_exon_Prss48_chr3_85997271_R GTCTCGTGGGCTCGGAGTGAGGCGTGTGAACAACT 59.8 0.01
mm4_intron_Arf3_chr15_98762304_F TCGTCGGCAGCGTCAACTCAGGGCCAGTGACAAG 59.8 0.00
mm4_intron_Arf3_chr15_98762304_R GTCTCGTGGGCTCGGGAGCTAGCTCGGAGGAGAGA 59.8 0.00
mm4_intergenic_Gm26180|Anks1b_chr10_89873013_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26180|Anks1b_chr10_89873013_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Nfia|Nfia/E130114P18Rik_chr4_97775151_F TCGTCGGCAGCGTCGCTGTAGTTGGAACTGGGCT 59.9 0.00
mm4_intergenic_Nfia|Nfia/E130114P18Rik_chr4_97775151_R GTCTCGTGGGCTCGGCAAGTTCAGCACCAAAGCCC 59.9 0.00
mm4_intron_Gng10_chr4_59035583_F TCGTCGGCAGCGTCGTGGGCGTGTCTACAACAGG 60.9 0.00
mm4_intron_Gng10_chr4_59035583_R GTCTCGTGGGCTCGGTCTGTTCATTCGGTGGCCTC 60.0 0.00
mm4_intergenic_Gtl3|Csnk2a2_chr8_95434928_F TCGTCGGCAGCGTCCCGCAGAAAGAACGGTTTCC 59.7 0.00
mm4_intergenic_Gtl3|Csnk2a2_chr8_95434928_R GTCTCGTGGGCTCGGGGAATAGTCCCTTGGCAACCA 59.9 0.00
mm4_intergenic_Grasp|Nr4a1_chr15_101254416_F TCGTCGGCAGCGTCTCAACGTGGTCCCCTCCTAC 60.9 0.00
mm4_intergenic_Grasp|Nr4a1_chr15_101254416_R GTCTCGTGGGCTCGGAAGCCCGAATGGATGGACAG 60.1 0.00
mm4_exon_Ntf5_chr7_45415661_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Ntf5_chr7_45415661_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm0_exon_Pop5_chr5_115238021 GAGCGTGGAGATTGACAGGGCACGCTGCCTGTCCGAGCCTAGTGCCGCCTTCCTCCCGCATCCGGCGCGGACGCCATGGT
GCGGTTCAAGCACAGGTAA
mm4_exon_Usp32_chr11_85139816 GGGTCGGAGCCTGCTTTCGCCCCCACCCCCCTCCCGCCTCCTTCGCGGCGTCGCCGGCTGACGGTGTCGGCGTCCCCGGC
CCCCTCCTCCCCCAACACTAACAAGTGCGGCTTCTGC
mm4_exon_Cnnm1_chr19_43440428 CTTGCTGCAGTATCACGTGCAACCGCGCCGGGTGCAGGATNNNNNNNNNNNNNNNNNNNNNAGCAGCGCTGGGTGTCAGG
CTCCGGGACTGCTGCAGCCGGGGCGCCGTACTCCTGCTCTTCTT
mm4_exon_Acaca_chr11_84391631 TGCAGTTTGCTGACTTGCACGACACACCAGGCCGGATGCAGGAGAAGGGTGTCATTAACGTAAGTAACACAATTGCCATT
TCTGACTTGTCAAAGCCTTGTAAGTGACATAGGCGCACAAGCTG
mm4_intergenic_1500009L16Rik|Nuak1_chr10_84029650 GCCTTCTGAGCTGTGTGGATCAGAAGACAAAAGCCAGATGATAACATGCATACAGCAAAGAAGGGCTTTCAATCCCAAGC
AGGATGAGGGAGGAGGG
AAATAACAAGGTATCTACAGCTGTGTGAAGCCTCG
mm3_intron_Oca2_chr7_56390704 TATCAGCTCAGCCAGTCAGGCTTCAACGTGCAGGATGCGGGAGGATAGGAGAGCAGCCATTGCTAGGGGCTCTCTCTGGG
TATG
mm4_intron_Ephb3_chr16_21210983 CTCCCTCCCTCTCCAGAAATTAAACAGAAATGAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNTCCTCCCGGAGCCAGCGCAGGGCTTGTTTTAAACTGTGGAGG
mm3_exon_Taf10_chr7_105744052 CGGCACCTAGTCCGAGATGCCCTCCTCCCTCACCCGGCCCGGCCCTCCCTCACCTGGCCCGCGCCGCTCTGCCGGCTCCC
CTGCCCGTGCCGCCACGGGGCCCGTGCCCGCAGTGGCGACTCCAGCCA
mm4_exon_Lphn3_chr5_81020803 TTTGGTCTCCTCAGCAGCTGCTTTTCTTNNNNNNNNNNNNNNNNNNNNNNNTGCAGGAGGAGGGAAAGCAGCTCTGGTTA
TGACTGCCGCATGT
mm4_exon_Wbscr27_chr5_134932652 CTGCCATCTCCCACTCAAGGTCTTGTTTGTAGCTTGGGACCGCGTTGGAGCACTGATAACCTCCTCCGCCGGGTGAGGGA
GGACGG
AAGACTGTGGAGGACCTCATCACTGGGTGGACT
mm4_intergenic_4930529M08Rik|Insm1_chr2_146221517 TGATTTTCCATACGACGCGCGGAGCTCCCGCGCCCTTCTCCCGCAGCAGCCGCGGCCCTCGGGTCGCCCCTGCCCCCACC
CCATCAGCGCGCCCAATTTCCCCCTGGGCTCCGGCTCGCGGATTGAACCCTCCTGACA
mm4_intergenic_Gm25784|Vstm2b_chr7_40898618 GAGAGATGGTGGTCGCTACGAATGACCCCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNATGCTGTGGAGGAGGCTGCTC
mm4_exon_Dlgap4_chr2_156721065 CCTCTCGGGCGTACAAAACCGGAGCCTCGGGCCGGGCCGCGTGAGGGAGGAGGGTGAGTGCCCGCCGCTGGCCGCAGCTG
CCGCCAGTCCATCCGTCACTCCG
mm4_intergenic_Gm20109|E230001N04Rik_chr17_28523190 CCCGAGCGAAAGAACATCCTGTGCCGCCGCCGGATGCGAGAGGCGGGGGCGCGGGGGAAGCTGGGCGATGCGCTCCGCCC
CGCGCTCCTCCTCCCGGGCGCTGCCCGCTCCCGCGCTTCGTACTGCTTCTTC
mm4_intron_Slc1a3_chr15_8638837 TATGGAGTGGGAGGGAGAGCAGCCAGATGTGCCCCGCCCGCCGGCTGAGGGAGGAGGGAGTTTGCCTGACATGTTAGGAT
GGGACTCTAGGCAAGGACT
mm4_exon_Bcor_chrX_12080181 GAGCTTCAGGGCAGGTGGCGACGGCCCCGCGCGATCCTCTCCTCCCGCAGCTGGCCCGAGCTCTGTCACCCTCAGCAG
mm4_exon_Pcdh10_chr3_45379856 ACTGGACCGAGAACAGCAAGCGGTGCACCGCTACGTGCTGACCGCGGTGGACNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNCCGGCCTGCCCCCCCAGCAGCAGCGTACTG
mm4_intergenic_Lima1|Gm9763_chr15_99875485 GCTGCAGCACCTTCAGCTNNNNNNNNNNNNNNNNNNNNNNNTCAGAGCCCCGCCCCCTGCCGCGGAGGCGCGCCCGTCTC
CCGCCTCCCGCGCGG
CCTTGGAGCGAACGTGCT
mm4_exon_4930503L19Rik/Stard6_chr18_70472517 CGCTTTCAAACCGACCCAACATGGCGCCGGGCCGCAGGGCGCATGTGTAGTACCAGGCGCCGGGAGGGATGGGGGAGGAG
GG
TCTCGAGAGCCCGGCGAAGAGTTCAGAGGTCAAAGGGCG
mm4_exon_Pou3f1_chr4_124660183 CAAGAACCTTGCGCCTGTTTCCCTACCGCTTCCCCCTGCTCTCTTTCCGGCCCCCGCGGGATGCGGGATGCTGGGGGAAC
CCTGGGGAGAAATTCTCCAAATTTGGGGTGAGGTGGGAAC
mm4_intron_Ankmy2_chr12_36157856 TAGGCTCCAAGTCGGGTGTATAAAGGGCCCTTTCGACTTCCGGAGGCTTAAGGATCTGGATCCCTTGCAGATCCCCGCCC
CTGCTGCGGGTGGAGGG
AGCTTTCAGCAACAGGGGTT
mm3_exon_Prss48_chr3_85997271 ATCCTTCCTCGACTGCCTCTCACCAGCACAGAACATGTCTTCCTTGATGACCCGCTCCAGATCCGGGAGGAAGACACCAA
TTGGGTTGTAGAGTTGTTCACACGCCTCACT
mm4_intron_Arf3_chr15_98762304 AACTCAGGGCCAGTGACAAGCTGCTCCCTCCTTCGGTGACACCGCCCCCGCTCCGCCTGGGGGAGGAAGGCCCCACCTCT
CTCCTCCGAGCTAGCTC
mm4_intergenic_Nfia|Nfia/E130114P18Rik_chr4_97775151 GCTGTAGTTGGAACTGGGCTTTGTAGTGTTCCCCCGCCTTCCACCCTCCTCCGGAGCGGGGGAGGGAGCGGGAGCTGGCA
CAGATAGGAGAGAAAGCAGGAGCGGATGTGGAGGGCTTTGGTGCTGAACTTG
mm4_intron_Gng10_chr4_59035583 GTGGGCGTGTCTACAACAGGCTGGCGACACGCTGGCGACCTGGACTCTGCCCACCCGGCTGCGGGAGGATGATGCTTAGA
GATGGGGAGGGAGGCCACCGAATGAACAGA
mm4_intergenic_Gtl3|Csnk2a2_chr8_95434928 CCGCAGAAAGAACGGTTTCCGGTTCCTTCCGCCCTTAGGCCCGCCCCTTCCTGCCCCCTCGGGCGCGGAACTTGGTTGCC
AAGGGACTATTCC
mm4_intergenic_Grasp|Nr4a1_chr15_101254416 TCAACGTGGTCCCCTCCTACCCGCCCCGGGGGCGGCAGGACGGGTGGGCGGCGCTGTGCTCGGCCAGGTCCGAGAGCCAG
CATCCCAGGCCACCCAGTGAGGGAAATGCTGTCCATCCATTCGGGCTT

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_ozJbN0W08qlgEBn3RvZK.txt --name MY_EXPERIMENT

Version 5.01 - Documentation  - Contact us - Downloads/local installation - Citation - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.