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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
Name | Primer Sequence | Tm | CFD Score |
---|---|---|---|
mm0_exon_Pop5_chr5_115238018_F | TCGTCGGCAGCGTCGAGCGTGGAGATTGACAGGG | 60.4 | 1.00 |
mm0_exon_Pop5_chr5_115238018_R | GTCTCGTGGGCTCGGTTACCTGTGCTTGAACCGCA | 60.1 | 1.00 |
mm4_intergenic_Alg10b|Cpne8_chr15_90461789_F | Primer3: not found at this Tm | N.d. | 0.65 |
mm4_intergenic_Alg10b|Cpne8_chr15_90461789_R | Primer3: not found at this Tm | N.d. | 0.65 |
mm4_intergenic_Tnp2|Prm3_chr16_10790235_F | Primer3: not found at this Tm | N.d. | 0.52 |
mm4_intergenic_Tnp2|Prm3_chr16_10790235_R | Primer3: not found at this Tm | N.d. | 0.52 |
mm4_intergenic_Kntc1|Niacr1_chr5_123824989_F | Primer3: not found at this Tm | N.d. | 0.51 |
mm4_intergenic_Kntc1|Niacr1_chr5_123824989_R | Primer3: not found at this Tm | N.d. | 0.51 |
mm4_intergenic_Fgfr2|Gm5903_chr7_130279582_F | TCGTCGGCAGCGTCTATGGGTCCCTCCTACAGGC | 60.1 | 0.50 |
mm4_intergenic_Fgfr2|Gm5903_chr7_130279582_R | GTCTCGTGGGCTCGGGCCCAGAAGAAACAGGAGCT | 59.9 | 0.50 |
mm4_exon_Kpna1_chr16_36000258_F | Primer3: not found at this Tm | N.d. | 0.50 |
mm4_exon_Kpna1_chr16_36000258_R | Primer3: not found at this Tm | N.d. | 0.50 |
mm4_intron_Ptprn2_chr12_116486724_F | TCGTCGGCAGCGTCGTGGATGGTAGGCAGTGAGG | 59.8 | 0.49 |
mm4_intron_Ptprn2_chr12_116486724_R | GTCTCGTGGGCTCGGCCTCCTCTCTGCAGTCCCTT | 60.6 | 0.49 |
mm4_intergenic_Myh9|Txn2_chr15_77888701_F | TCGTCGGCAGCGTCACAGAGCTCCAGGTAGAGGG | 60.0 | 0.45 |
mm4_intergenic_Myh9|Txn2_chr15_77888701_R | GTCTCGTGGGCTCGGCCGATCAGCCCTTTCTCTCC | 59.8 | 0.45 |
mm4_intron_Grm7_chr6_111500187_F | TCGTCGGCAGCGTCAGCCAAGATGCTGTTCCTCA | 59.3 | 0.42 |
mm4_intron_Grm7_chr6_111500187_R | GTCTCGTGGGCTCGGGGCATCTTCCAGTTTTCCATGT | 59.1 | 0.42 |
mm4_intergenic_Gm10620|Cmc2_chr8_116863675_F | TCGTCGGCAGCGTCGACATCCCCTGCAGATGGAG | 59.8 | 0.38 |
mm4_intergenic_Gm10620|Cmc2_chr8_116863675_R | GTCTCGTGGGCTCGGCGCGAGGGTCTAGTCTTCTC | 59.3 | 0.38 |
mm4_intergenic_Begain|Gm22234_chr12_109147018_F | Primer3: not found at this Tm | N.d. | 0.37 |
mm4_intergenic_Begain|Gm22234_chr12_109147018_R | Primer3: not found at this Tm | N.d. | 0.37 |
mm4_exon_Cklf_chr8_104263916_F | Primer3: not found at this Tm | N.d. | 0.36 |
mm4_exon_Cklf_chr8_104263916_R | Primer3: not found at this Tm | N.d. | 0.36 |
mm3_intergenic_Gm3839|Ptprg_chr14_11552736_F | TCGTCGGCAGCGTCTTTGCCCTCTGCCTAACCAG | 59.9 | 0.34 |
mm3_intergenic_Gm3839|Ptprg_chr14_11552736_R | GTCTCGTGGGCTCGGAAGGTCTGTCGCGGAGTTTT | 59.8 | 0.34 |
mm4_intergenic_Gm23752|Gm24815_chr12_7420266_F | Primer3: not found at this Tm | N.d. | 0.34 |
mm4_intergenic_Gm23752|Gm24815_chr12_7420266_R | Primer3: not found at this Tm | N.d. | 0.34 |
mm4_intergenic_Mdfic|Tfec_chr6_16545293_F | Primer3: not found at this Tm | N.d. | 0.32 |
mm4_intergenic_Mdfic|Tfec_chr6_16545293_R | Primer3: not found at this Tm | N.d. | 0.32 |
mm4_exon_Angel2_chr1_190928833_F | Primer3: not found at this Tm | N.d. | 0.32 |
mm4_exon_Angel2_chr1_190928833_R | Primer3: not found at this Tm | N.d. | 0.32 |
mm4_intron_Tet2_chr3_133510561_F | Primer3: not found at this Tm | N.d. | 0.31 |
mm4_intron_Tet2_chr3_133510561_R | Primer3: not found at this Tm | N.d. | 0.31 |
mm4_intron_4930465A12Rik_chr4_128155355_F | Primer3: not found at this Tm | N.d. | 0.31 |
mm4_intron_4930465A12Rik_chr4_128155355_R | Primer3: not found at this Tm | N.d. | 0.31 |
mm4_intergenic_Diap2|Gm14984_chrX_130389026_F | TCGTCGGCAGCGTCGCAACCCTACCTGTGCTCTC | 60.3 | 0.30 |
mm4_intergenic_Diap2|Gm14984_chrX_130389026_R | GTCTCGTGGGCTCGGGTTCACACAACTAAGCCATGGT | 59.1 | 0.30 |
mm4_intron_Maea_chr5_33365609_F | TCGTCGGCAGCGTCTGGCCTGCCAGAAGAAAACT | 59.8 | 0.29 |
mm4_intron_Maea_chr5_33365609_R | GTCTCGTGGGCTCGGATGGCCTAGGATCCACAGGT | 60.0 | 0.29 |
mm4_intergenic_CT030684.1|Gm23276_chr14_97311670_F | Primer3: not found at this Tm | N.d. | 0.28 |
mm4_intergenic_CT030684.1|Gm23276_chr14_97311670_R | Primer3: not found at this Tm | N.d. | 0.28 |
mm4_intergenic_Cd52|Ubxn11_chr4_134095082_F | Primer3: not found at this Tm | N.d. | 0.28 |
mm4_intergenic_Cd52|Ubxn11_chr4_134095082_R | Primer3: not found at this Tm | N.d. | 0.28 |
mm4_exon_Brix1_chr15_10475665_F | Primer3: not found at this Tm | N.d. | 0.22 |
mm4_exon_Brix1_chr15_10475665_R | Primer3: not found at this Tm | N.d. | 0.22 |
mm4_intergenic_Lalba|Olfr279_chr15_98491078_F | Primer3: not found at this Tm | N.d. | 0.22 |
mm4_intergenic_Lalba|Olfr279_chr15_98491078_R | Primer3: not found at this Tm | N.d. | 0.22 |
mm4_intergenic_Nfia|Nfia/E130114P18Rik_chr4_97775148_F | TCGTCGGCAGCGTCGCTGTAGTTGGAACTGGGCT | 59.9 | 0.21 |
mm4_intergenic_Nfia|Nfia/E130114P18Rik_chr4_97775148_R | GTCTCGTGGGCTCGGCAAGTTCAGCACCAAAGCCC | 59.9 | 0.21 |
mm4_intergenic_F730016J06Rik|Olfm1_chr2_28189175_F | TCGTCGGCAGCGTCACAAGGTTAGAGGAGGGGCA | 60.1 | 0.21 |
mm4_intergenic_F730016J06Rik|Olfm1_chr2_28189175_R | GTCTCGTGGGCTCGGAAGGCTAGCAGCTAGTGTGC | 60.1 | 0.21 |
mm4_exon_Gsk3b_chr16_38089430_F | Primer3: not found at this Tm | N.d. | 0.20 |
mm4_exon_Gsk3b_chr16_38089430_R | Primer3: not found at this Tm | N.d. | 0.20 |
mm3_intergenic_Agpat9|Gm20484_chr5_101361707_F | Primer3: not found at this Tm | N.d. | 0.19 |
mm3_intergenic_Agpat9|Gm20484_chr5_101361707_R | Primer3: not found at this Tm | N.d. | 0.19 |
mm4_intergenic_Gm2366/Fat1|Gm2366_chr8_45020786_F | TCGTCGGCAGCGTCAGGAGGAAAGAGAACACGTGT | 58.9 | 0.19 |
mm4_intergenic_Gm2366/Fat1|Gm2366_chr8_45020786_R | GTCTCGTGGGCTCGGTTCGTTCACCAGAATGCCCC | 60.6 | 0.19 |
mm4_intergenic_Gm14601/Gm15482|Gm14644_chrX_54091434_F | Primer3: not found at this Tm | N.d. | 0.18 |
mm4_intergenic_Gm14601/Gm15482|Gm14644_chrX_54091434_R | Primer3: not found at this Tm | N.d. | 0.18 |
mm4_intron_Epb4.1l3_chr17_69242615_F | Primer3: not found at this Tm | N.d. | 0.18 |
mm4_intron_Epb4.1l3_chr17_69242615_R | Primer3: not found at this Tm | N.d. | 0.18 |
mm4_intergenic_Gm24686|Zfp804b_chr5_6743193_F | Primer3: not found at this Tm | N.d. | 0.16 |
mm4_intergenic_Gm24686|Zfp804b_chr5_6743193_R | Primer3: not found at this Tm | N.d. | 0.16 |
mm4_intergenic_Foxp1|Gm24936_chr6_99522897_F | TCGTCGGCAGCGTCCTCTCAAAGCCCCTGTGACC | 60.3 | 0.16 |
mm4_intergenic_Foxp1|Gm24936_chr6_99522897_R | GTCTCGTGGGCTCGGCCTCTCTTGCGCGCCTTATT | 60.8 | 0.16 |
mm4_intergenic_PVT1_2|Pvt1_chr15_62095843_F | Primer3: not found at this Tm | N.d. | 0.15 |
mm4_intergenic_PVT1_2|Pvt1_chr15_62095843_R | Primer3: not found at this Tm | N.d. | 0.15 |
mm4_intergenic_Gm10076|Gm22406_chr14_105798288_F | Primer3: not found at this Tm | N.d. | 0.15 |
mm4_intergenic_Gm10076|Gm22406_chr14_105798288_R | Primer3: not found at this Tm | N.d. | 0.15 |
mm4_intron_Gm26791_chr14_118369252_F | Primer3: not found at this Tm | N.d. | 0.14 |
mm4_intron_Gm26791_chr14_118369252_R | Primer3: not found at this Tm | N.d. | 0.14 |
mm4_exon_Prss41/Prss32_chr17_23843721_F | Primer3: not found at this Tm | N.d. | 0.14 |
mm4_exon_Prss41/Prss32_chr17_23843721_R | Primer3: not found at this Tm | N.d. | 0.14 |
mm3_intron_Usp6nl_chr2_6323263_F | Primer3: not found at this Tm | N.d. | 0.13 |
mm3_intron_Usp6nl_chr2_6323263_R | Primer3: not found at this Tm | N.d. | 0.13 |
mm4_intron_Baiap2l2_chr15_79268641_F | TCGTCGGCAGCGTCGTGGCTCTATTAAGGATGTTTCAAA | 57.6 | 0.13 |
mm4_intron_Baiap2l2_chr15_79268641_R | GTCTCGTGGGCTCGGCCCTGCAGAAGTGAGTTCCA | 59.6 | 0.13 |
mm4_intron_Bri3bp_chr5_125443865_F | Primer3: not found at this Tm | N.d. | 0.13 |
mm4_intron_Bri3bp_chr5_125443865_R | Primer3: not found at this Tm | N.d. | 0.13 |
mm4_intergenic_2310050C09Rik|Lce3a_chr3_92893518_F | Primer3: not found at this Tm | N.d. | 0.13 |
mm4_intergenic_2310050C09Rik|Lce3a_chr3_92893518_R | Primer3: not found at this Tm | N.d. | 0.13 |
mm4_intron_Acad12_chr5_121614594_F | TCGTCGGCAGCGTCGACCCCAATCTCCTGCCTTC | 60.1 | 0.13 |
mm4_intron_Acad12_chr5_121614594_R | GTCTCGTGGGCTCGGACATCTGCGGCTTTGAAGGA | 59.9 | 0.13 |
mm4_intron_Acad10_chr5_121656551_F | TCGTCGGCAGCGTCGACCCCAATCTCCTGCCTTC | 60.1 | 0.13 |
mm4_intron_Acad10_chr5_121656551_R | GTCTCGTGGGCTCGGACATCTGCGGCTTTGAAGGA | 59.9 | 0.13 |
mm4_intron_9530036O11Rik_chr5_28816579_F | Primer3: not found at this Tm | N.d. | 0.12 |
mm4_intron_9530036O11Rik_chr5_28816579_R | Primer3: not found at this Tm | N.d. | 0.12 |
mm4_exon_Pdcd7_chr9_65346298_F | Primer3: not found at this Tm | N.d. | 0.11 |
mm4_exon_Pdcd7_chr9_65346298_R | Primer3: not found at this Tm | N.d. | 0.11 |
mm4_exon_Arhgap1_chr2_91653205_F | Primer3: not found at this Tm | N.d. | 0.11 |
mm4_exon_Arhgap1_chr2_91653205_R | Primer3: not found at this Tm | N.d. | 0.11 |
mm4_intergenic_Pccb|Msl2_chr9_101053423_F | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_Pccb|Msl2_chr9_101053423_R | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_Gm25574|Ankrd50_chr3_38410198_F | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_Gm25574|Ankrd50_chr3_38410198_R | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_Gm12536|1700042G15Rik_chr4_57315172_F | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_Gm12536|1700042G15Rik_chr4_57315172_R | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_6820408C15Rik|Gm14158_chr2_152450280_F | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_6820408C15Rik|Gm14158_chr2_152450280_R | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intron_Fam114a1_chr5_65033215_F | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intron_Fam114a1_chr5_65033215_R | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intron_Fst_chr13_114456707_F | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intron_Fst_chr13_114456707_R | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_Ppp1r15b|Plekha6_chr1_133192458_F | TCGTCGGCAGCGTCCTCTCTTATGGCTGCTCGGG | 59.9 | 0.10 |
mm4_intergenic_Ppp1r15b|Plekha6_chr1_133192458_R | GTCTCGTGGGCTCGGCCCAGCCCCACATGAATTGA | 60.3 | 0.10 |
mm4_intergenic_Gm26419|Pak2_chr16_32079015_F | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_Gm26419|Pak2_chr16_32079015_R | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_Gm7308|Parp11_chr6_127425070_F | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_Gm7308|Parp11_chr6_127425070_R | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_Dppa3|Gm10420_chr6_122640581_F | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_intergenic_Dppa3|Gm10420_chr6_122640581_R | Primer3: not found at this Tm | N.d. | 0.10 |
mm4_exon_AI480526_chr5_123141451_F | Primer3: not found at this Tm | N.d. | 0.09 |
mm4_exon_AI480526_chr5_123141451_R | Primer3: not found at this Tm | N.d. | 0.09 |
mm4_exon_Camk2n1_chr4_138454356_F | Primer3: not found at this Tm | N.d. | 0.09 |
mm4_exon_Camk2n1_chr4_138454356_R | Primer3: not found at this Tm | N.d. | 0.09 |
mm4_intergenic_Gm13282|Klhl9/Gm26566_chr4_88708672_F | TCGTCGGCAGCGTCCAGTGTTGCTCAGGTGTTTGG | 59.9 | 0.09 |
mm4_intergenic_Gm13282|Klhl9/Gm26566_chr4_88708672_R | GTCTCGTGGGCTCGGAAGGATAACACTTTACAGGTGAGT | 57.5 | 0.09 |
mm4_exon_Adam12_chr7_134225247_F | TCGTCGGCAGCGTCGCCGTTCTCATTACCACGGA | 60.1 | 0.09 |
mm4_exon_Adam12_chr7_134225247_R | GTCTCGTGGGCTCGGGAGTGGATAGGACCCAGGCT | 60.3 | 0.09 |
mm3_intergenic_Negr1|Gm22458_chr3_157327222_F | Primer3: not found at this Tm | N.d. | 0.08 |
mm3_intergenic_Negr1|Gm22458_chr3_157327222_R | Primer3: not found at this Tm | N.d. | 0.08 |
mm4_intergenic_Ap3m2|1700041G16Rik_chr8_22806747_F | Primer3: not found at this Tm | N.d. | 0.07 |
mm4_intergenic_Ap3m2|1700041G16Rik_chr8_22806747_R | Primer3: not found at this Tm | N.d. | 0.07 |
mm4_intergenic_Dmrt2|Gm8825_chr19_25771835_F | Primer3: not found at this Tm | N.d. | 0.07 |
mm4_intergenic_Dmrt2|Gm8825_chr19_25771835_R | Primer3: not found at this Tm | N.d. | 0.07 |
mm4_intergenic_4930404H24Rik|8430427H17Rik_chr2_153528284_F | Primer3: not found at this Tm | N.d. | 0.07 |
mm4_intergenic_4930404H24Rik|8430427H17Rik_chr2_153528284_R | Primer3: not found at this Tm | N.d. | 0.07 |
mm4_intron_Prmt2_chr10_76234140_F | Primer3: not found at this Tm | N.d. | 0.06 |
mm4_intron_Prmt2_chr10_76234140_R | Primer3: not found at this Tm | N.d. | 0.06 |
mm4_intergenic_Gm9901/Ccsap|Acta1_chr8_123878274_F | Primer3: not found at this Tm | N.d. | 0.06 |
mm4_intergenic_Gm9901/Ccsap|Acta1_chr8_123878274_R | Primer3: not found at this Tm | N.d. | 0.06 |
mm4_intron_Mrps31_chr8_22415854_F | Primer3: not found at this Tm | N.d. | 0.06 |
mm4_intron_Mrps31_chr8_22415854_R | Primer3: not found at this Tm | N.d. | 0.06 |
mm4_exon_Ergic3_chr2_156008080_F | TCGTCGGCAGCGTCCTTTGAGGAGGCAGGTGAGG | 60.0 | 0.06 |
mm4_exon_Ergic3_chr2_156008080_R | GTCTCGTGGGCTCGGGGAAAGGGGACACCAGCC | 59.9 | 0.06 |
mm4_intron_Mmd_chr11_90275268_F | Primer3: not found at this Tm | N.d. | 0.06 |
mm4_intron_Mmd_chr11_90275268_R | Primer3: not found at this Tm | N.d. | 0.06 |
mm4_intergenic_Il1rapl1|Gm25006_chrX_88192364_F | Primer3: not found at this Tm | N.d. | 0.06 |
mm4_intergenic_Il1rapl1|Gm25006_chrX_88192364_R | Primer3: not found at this Tm | N.d. | 0.06 |
mm4_intron_Fetub_chr16_22920126_F | TCGTCGGCAGCGTCAGCTTTCTTGCTGCGTCATG | 59.4 | 0.05 |
mm4_intron_Fetub_chr16_22920126_R | GTCTCGTGGGCTCGGCCAAGGGCTCTGTTCTCAGG | 60.0 | 0.05 |
mm4_intergenic_Bmpr2|Fam117b_chr1_59888998_F | Primer3: not found at this Tm | N.d. | 0.05 |
mm4_intergenic_Bmpr2|Fam117b_chr1_59888998_R | Primer3: not found at this Tm | N.d. | 0.05 |
mm4_intergenic_Adamts5|Gm25715_chr16_86240953_F | Primer3: not found at this Tm | N.d. | 0.05 |
mm4_intergenic_Adamts5|Gm25715_chr16_86240953_R | Primer3: not found at this Tm | N.d. | 0.05 |
mm4_intergenic_AU022754|Mirlet7c-2_chr15_85656265_F | TCGTCGGCAGCGTCGCCAGGCTCCCAACTTGTAA | 60.2 | 0.05 |
mm4_intergenic_AU022754|Mirlet7c-2_chr15_85656265_R | GTCTCGTGGGCTCGGCTCAGTCCGCTCCCTTTGAA | 59.6 | 0.05 |
mm3_intron_Trmt1_chr8_84694688_F | Primer3: not found at this Tm | N.d. | 0.05 |
mm3_intron_Trmt1_chr8_84694688_R | Primer3: not found at this Tm | N.d. | 0.05 |
mm4_exon_Zfp521_chr18_13972702_F | Primer3: not found at this Tm | N.d. | 0.05 |
mm4_exon_Zfp521_chr18_13972702_R | Primer3: not found at this Tm | N.d. | 0.05 |
mm4_intron_Trim37_chr11_87177604_F | Primer3: not found at this Tm | N.d. | 0.05 |
mm4_intron_Trim37_chr11_87177604_R | Primer3: not found at this Tm | N.d. | 0.05 |
mm4_intergenic_Got2|Gm22780_chr8_96306036_F | Primer3: not found at this Tm | N.d. | 0.04 |
mm4_intergenic_Got2|Gm22780_chr8_96306036_R | Primer3: not found at this Tm | N.d. | 0.04 |
mm4_intergenic_4933434M16Rik|2610035D17Rik_chr11_112941984_F | Primer3: not found at this Tm | N.d. | 0.04 |
mm4_intergenic_4933434M16Rik|2610035D17Rik_chr11_112941984_R | Primer3: not found at this Tm | N.d. | 0.04 |
mm4_intergenic_Kat6a|Ank1_chr8_22974783_F | Primer3: not found at this Tm | N.d. | 0.04 |
mm4_intergenic_Kat6a|Ank1_chr8_22974783_R | Primer3: not found at this Tm | N.d. | 0.04 |
mm4_intergenic_Cdh11|Gm8730_chr8_102803371_F | Primer3: not found at this Tm | N.d. | 0.04 |
mm4_intergenic_Cdh11|Gm8730_chr8_102803371_R | Primer3: not found at this Tm | N.d. | 0.04 |
mm4_intergenic_Immp2l|Gm25497_chr12_41221244_F | Primer3: not found at this Tm | N.d. | 0.04 |
mm4_intergenic_Immp2l|Gm25497_chr12_41221244_R | Primer3: not found at this Tm | N.d. | 0.04 |
mm4_intron_4931406P16Rik_chr7_34261863_F | TCGTCGGCAGCGTCGTGTGGGATCTGGCTGATGA | 59.4 | 0.03 |
mm4_intron_4931406P16Rik_chr7_34261863_R | GTCTCGTGGGCTCGGTGCCATAGGAGAAGAGGCTG | 58.8 | 0.03 |
mm4_intergenic_Sec11a|Zfp592_chr7_80993452_F | Primer3: not found at this Tm | N.d. | 0.03 |
mm4_intergenic_Sec11a|Zfp592_chr7_80993452_R | Primer3: not found at this Tm | N.d. | 0.03 |
mm4_intron_Gm13318_chr2_16084400_F | Primer3: not found at this Tm | N.d. | 0.03 |
mm4_intron_Gm13318_chr2_16084400_R | Primer3: not found at this Tm | N.d. | 0.03 |
mm4_intergenic_Gm11445|Gm11446_chr2_159856471_F | Primer3: not found at this Tm | N.d. | 0.03 |
mm4_intergenic_Gm11445|Gm11446_chr2_159856471_R | Primer3: not found at this Tm | N.d. | 0.03 |
mm4_intron_Slc2a1_chr4_119109043_F | Primer3: not found at this Tm | N.d. | 0.03 |
mm4_intron_Slc2a1_chr4_119109043_R | Primer3: not found at this Tm | N.d. | 0.03 |
mm4_intergenic_Ppp1r14c|Iyd_chr10_3539440_F | TCGTCGGCAGCGTCAGCCACAAGGGAAGCTTTCT | 59.5 | 0.03 |
mm4_intergenic_Ppp1r14c|Iyd_chr10_3539440_R | GTCTCGTGGGCTCGGCCTCGCTTGTATTGTTCTCACA | 58.6 | 0.03 |
mm4_intergenic_Cd44|Pdhx_chr2_102916041_F | TCGTCGGCAGCGTCAGAGCCAAGGCAGAGAGAGT | 60.2 | 0.03 |
mm4_intergenic_Cd44|Pdhx_chr2_102916041_R | GTCTCGTGGGCTCGGTGGGGACTTGGAGAGGCTTT | 60.7 | 0.03 |
mm4_intron_Ppard_chr17_28251709_F | TCGTCGGCAGCGTCGGAAGTTGTGGGTGAGGAGG | 59.9 | 0.03 |
mm4_intron_Ppard_chr17_28251709_R | GTCTCGTGGGCTCGGACAGCCTCTTCCCCACTAGT | 59.8 | 0.03 |
mm4_intron_Pik3c2b_chr1_133054791_F | Primer3: not found at this Tm | N.d. | 0.03 |
mm4_intron_Pik3c2b_chr1_133054791_R | Primer3: not found at this Tm | N.d. | 0.03 |
mm4_intergenic_Nim1|Zfp131_chr13_119760793_F | TCGTCGGCAGCGTCTGCAGCAATCCTGTTTTCACA | 58.9 | 0.03 |
mm4_intergenic_Nim1|Zfp131_chr13_119760793_R | GTCTCGTGGGCTCGGGCACCCCAACTCATCAGTCA | 59.9 | 0.03 |
mm4_intergenic_Sel1l3|Smim20_chr5_53259316_F | TCGTCGGCAGCGTCAGCACAAACCAGACCCCAAA | 60.0 | 0.03 |
mm4_intergenic_Sel1l3|Smim20_chr5_53259316_R | GTCTCGTGGGCTCGGGAGATCTTCCCTCCCCAGGT | 60.0 | 0.03 |
mm3_intergenic_Gm9884|Gm24064_chr1_25971714_F | Primer3: not found at this Tm | N.d. | 0.03 |
mm3_intergenic_Gm9884|Gm24064_chr1_25971714_R | Primer3: not found at this Tm | N.d. | 0.03 |
mm4_intron_Mrps27_chr13_99357127_F | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intron_Mrps27_chr13_99357127_R | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intron_Ssbp3_chr4_106911939_F | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intron_Ssbp3_chr4_106911939_R | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intron_Itpr1_chr6_108213944_F | TCGTCGGCAGCGTCCGGGGAAACAAGTGCTTTGG | 59.9 | 0.02 |
mm4_intron_Itpr1_chr6_108213944_R | GTCTCGTGGGCTCGGCGTCAGTAGCTGGCCAGAAA | 60.0 | 0.02 |
mm3_intergenic_Gm22227|Zfp703_chr8_26830241_F | Primer3: not found at this Tm | N.d. | 0.02 |
mm3_intergenic_Gm22227|Zfp703_chr8_26830241_R | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intron_Eno2_chr6_124766452_F | TCGTCGGCAGCGTCGGCTCAGGTCTCAAGTTCCC | 60.0 | 0.02 |
mm4_intron_Eno2_chr6_124766452_R | GTCTCGTGGGCTCGGTGAGCACTGTACTTGCCCAG | 59.9 | 0.02 |
mm4_intron_Cntnap2_chr6_46405778_F | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intron_Cntnap2_chr6_46405778_R | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_exon_Abcb4_chr5_8893813_F | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_exon_Abcb4_chr5_8893813_R | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intergenic_Gm26619/Gm10260|Arhgef28_chr13_97895721_F | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intergenic_Gm26619/Gm10260|Arhgef28_chr13_97895721_R | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intergenic_Fbxw10|Tvp23b_chr11_62879356_F | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intergenic_Fbxw10|Tvp23b_chr11_62879356_R | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intron_Rara_chr11_98969510_F | TCGTCGGCAGCGTCTGCAGAATCGACATGAGTGATCT | 59.8 | 0.02 |
mm4_intron_Rara_chr11_98969510_R | GTCTCGTGGGCTCGGCAATCACTCACAGGGCCTCC | 60.3 | 0.02 |
mm4_exon_Dscam_chr16_96709102_F | TCGTCGGCAGCGTCGAGTGAACTTCTGGGTGGCA | 59.8 | 0.02 |
mm4_exon_Dscam_chr16_96709102_R | GTCTCGTGGGCTCGGACCGGATTGGCAAGAGTGAG | 60.0 | 0.02 |
mm4_intron_Npas2_chr1_39330112_F | TCGTCGGCAGCGTCTCTTTTCCAGTGGGCTGCTC | 60.2 | 0.02 |
mm4_intron_Npas2_chr1_39330112_R | GTCTCGTGGGCTCGGTCAGTCCTCATGCTCTCCCA | 59.9 | 0.02 |
mm4_intergenic_Nsmce2|Trib1_chr15_59641059_F | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intergenic_Nsmce2|Trib1_chr15_59641059_R | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intron_Sppl2a_chr2_126932883_F | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intron_Sppl2a_chr2_126932883_R | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intergenic_Gm10030|Ltf_chr9_111015377_F | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intergenic_Gm10030|Ltf_chr9_111015377_R | Primer3: not found at this Tm | N.d. | 0.02 |
mm3_intergenic_RP23-433F5.6|Cbln1_chr8_87468803_F | Primer3: not found at this Tm | N.d. | 0.02 |
mm3_intergenic_RP23-433F5.6|Cbln1_chr8_87468803_R | Primer3: not found at this Tm | N.d. | 0.02 |
mm4_intergenic_4930527J03Rik|B230369F24Rik_chr1_178299785_F | Primer3: not found at this Tm | N.d. | 0.01 |
mm4_intergenic_4930527J03Rik|B230369F24Rik_chr1_178299785_R | Primer3: not found at this Tm | N.d. | 0.01 |
mm4_exon_Lphn1_chr8_83900088_F | TCGTCGGCAGCGTCTAGTCCAGGCCAGGTTTTGC | 60.2 | 0.01 |
mm4_exon_Lphn1_chr8_83900088_R | GTCTCGTGGGCTCGGGCACCAGCAATGACAAGCC | 60.0 | 0.01 |
mm4_intron_Wbscr27_chr5_134937008_F | Primer3: not found at this Tm | N.d. | 0.01 |
mm4_intron_Wbscr27_chr5_134937008_R | Primer3: not found at this Tm | N.d. | 0.01 |
mm4_intergenic_Mark3|Ckb_chr12_111666393_F | Primer3: not found at this Tm | N.d. | 0.01 |
mm4_intergenic_Mark3|Ckb_chr12_111666393_R | Primer3: not found at this Tm | N.d. | 0.01 |
mm4_intergenic_Defb34|Spag11b_chr8_19128040_F | TCGTCGGCAGCGTCTGACTAGAGGCCCAGGAAAA | 57.9 | 0.01 |
mm4_intergenic_Defb34|Spag11b_chr8_19128040_R | GTCTCGTGGGCTCGGGTGCAGTAAGAGAAGGGCAGA | 59.7 | 0.01 |
mm4_intergenic_Wdr66|Gm15857/Wdr66_chr5_123292265_F | TCGTCGGCAGCGTCGCGGAAGGTAGAATCTGGCA | 59.8 | 0.01 |
mm4_intergenic_Wdr66|Gm15857/Wdr66_chr5_123292265_R | GTCTCGTGGGCTCGGGCCTTTGCTCAGGAGAGTGT | 59.9 | 0.01 |
mm4_exon_Pou3f1_chr4_124660186_F | Primer3: not found at this Tm | N.d. | 0.00 |
mm4_exon_Pou3f1_chr4_124660186_R | Primer3: not found at this Tm | N.d. | 0.00 |
mm4_intergenic_Gm24839|Tmem132c_chr5_126756844_F | Primer3: not found at this Tm | N.d. | 0.00 |
mm4_intergenic_Gm24839|Tmem132c_chr5_126756844_R | Primer3: not found at this Tm | N.d. | 0.00 |
mm4_exon_Csnk2a2_chr8_95446343_F | Primer3: not found at this Tm | N.d. | 0.00 |
mm4_exon_Csnk2a2_chr8_95446343_R | Primer3: not found at this Tm | N.d. | 0.00 |
mm4_intron_Nrtn_chr17_56755131_F | TCGTCGGCAGCGTCGGAAGGGAAACTGAGGCAGG | 60.3 | 0.00 |
mm4_intron_Nrtn_chr17_56755131_R | GTCTCGTGGGCTCGGGAGGCTCGGAGAACAGACAC | 60.1 | 0.00 |
mm4_intron_Il17rd_chr14_27039327_F | TCGTCGGCAGCGTCTCTCCGGGTTCCAGGTTACC | 60.9 | 0.00 |
mm4_intron_Il17rd_chr14_27039327_R | GTCTCGTGGGCTCGGGCAGGATGAGCGGCTAGAAA | 60.1 | 0.00 |
mm4_intergenic_Gm5250|Prdm14_chr1_13096001_F | TCGTCGGCAGCGTCTGATGCAGCTGGAAGATATGAGA | 59.3 | 0.00 |
mm4_intergenic_Gm5250|Prdm14_chr1_13096001_R | GTCTCGTGGGCTCGGGGGCCCTCTTTCTGCTTTTG | 59.3 | 0.00 |
mm4_exon_Ptp4a2_chr4_129820547_F | Primer3: not found at this Tm | N.d. | 0.00 |
mm4_exon_Ptp4a2_chr4_129820547_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
mm0_exon_Pop5_chr5_115238018 |
GAGCGTGGAGATTGACAGGGCACGCTGCCTGTCCGAGCCTAGTGCCGCCTTCCTCCCGCATCCGGCGCGGACGCCATGGT GCGGTTCAAGCACAGGTAA |
mm4_intergenic_Fgfr2|Gm5903_chr7_130279582 |
TATGGGTCCCTCCTACAGGCCTGTGTGGAGCAGTTCCGCTCTGAGGAATCGGTCCCTGTTAGGCTGGAGGCGGGAGGAAG AAGGGGAAACTCAAGCGGAAAATCTTTGTTACAGCTCCTGTTTCTTCTGGGC |
mm4_intron_Ptprn2_chr12_116486724 |
GTGGATGGTAGGCAGTGAGGGGTGAGCTGCAGCCTTGGCCCGAGCTCCCCTCAGGCTCCACACCTCCTTCCTCCCGCCTC TGGTCTTCAGAGGTGTTTCAACCTTGCAAAACTCAAAGAAAGGGACTGCAGAGAGGAGG |
mm4_intergenic_Myh9|Txn2_chr15_77888701 |
ACAGAGCTCCAGGTAGAGGGATCACCATGAACAAAGGAGGCGGGAGGAAGGAGGCTGGAAGGCACATGTAGTGTGGTCCT GGTCCTGGAGAGAAAGGGCTGATCGG |
mm4_intron_Grm7_chr6_111500187 |
AGCCAAGATGCTGTTCCTCAGAATTTCTTCAAATTCTGGTCCTCCCACCTTCCTCCTGAATCTGGCCTCTCTCCCTGTAC TTCCAAATGTGAACGTGTGTATATACATGGAAAACTGGAAGATGCC |
mm4_intergenic_Gm10620|Cmc2_chr8_116863675 |
GACATCCCCTGCAGATGGAGGCCAGGCTTCGAGAAAGAGGACCAACCGTCTTCCTCCCGCCTACTGCGGTAGCCCCACGG ACCTCTCCTTTCACCTTGGAGAAGACTAGACCCTCGCG |
mm3_intergenic_Gm3839|Ptprg_chr14_11552736 |
TTTGCCCTCTGCCTAACCAGGCCAAGTTGCAGCCACCTTCTTCCCGAATCCGGGGGTTGGGAAAACTCCGCGACAGACCT T |
mm4_intergenic_Diap2|Gm14984_chrX_130389026 |
GCAACCCTACCTGTGCTCTCTATTGAATTGCACTTCCTGCTTCTGCCACCATCCTCTCACATCCGGGGCTACTACCTCTG AAATCTTTAGATTATAAAGTCACAATCATCTAAAACCATGGCTTAGTTGTGTGAAC |
mm4_intron_Maea_chr5_33365609 |
TGGCCTGCCAGAAGAAAACTTTTGCCACTTCTGTCAGAATAGAAGCTGTGGTAATCCCACTTCTTCCCGCACCCGGAGCT TCAATGGTAGAAATTGTGAGACCTGTGGATCCTAGGCCAT |
mm4_intergenic_Nfia|Nfia/E130114P18Rik_chr4_97775148 |
GCTGTAGTTGGAACTGGGCTTTGTAGTGTTCCCCCGCCTTCCACCCTCCTCCGGAGCGGGGGAGGGAGCGGGAGCTGGCA CAGATAGGAGAGAAAGCAGGAGCGGATGTGGAGGGCTTTGGTGCTGAACTTG |
mm4_intergenic_F730016J06Rik|Olfm1_chr2_28189175 | ACAAGGTTAGAGGAGGGGCAGGAGTGAAACACTGGAGGCCGGAGGAAGGAGGAACTGAGCACACTAGCTGCTAGCCTT |
mm4_intergenic_Gm2366/Fat1|Gm2366_chr8_45020786 |
AGGAGGAAAGAGAACACGTGTATAATAAAAAATAAACGTAGCCACACCGGATAGGGAAGGAAGGAGGAGTCTTCAGGCAG GGGCATTCTGGTGAACGAA |
mm4_intergenic_Foxp1|Gm24936_chr6_99522897 |
CTCTCAAAGCCCCTGTGACCCGCCGAGCGCGATCTGCACCCCGATGCGGCGCCCTTGGGTCGCCTGCCCCCTTCCCCCCG CATCCCGGAACAAATAAATAAATAAGGCGCGCAAGAGAGG |
mm4_intron_Baiap2l2_chr15_79268641 |
GTGGCTCTATTAAGGATGTTTCAAAGAATAGCCTCCTTCCTCCAGCAACCAGCAGGGATTGGACATCCAGACAATGGAAC TCACTTCTGCAGGG |
mm4_intron_Acad12_chr5_121614594 |
GACCCCAATCTCCTGCCTTCTTCTCTCACTGCAGATGTCTCTTCAGGGACCCTGGTGGCTCCCTGCTCCTTCCTCCTTCC TCCCTCATCAGCCAGAGAATTCCTTCAAAGCCGCAGATGT |
mm4_intron_Acad10_chr5_121656551 |
GACCCCAATCTCCTGCCTTCTTCTCTCACTGCAGATGTCTCTTCAGGACCCTGGTGGCTCCCTGCTCCTTCCTCCTTCCT CCCTCATCAGCCAGAGAAATCCTTCAAAGCCGCAGATGT |
mm4_intergenic_Ppp1r15b|Plekha6_chr1_133192458 |
CTCTCTTATGGCTGCTCGGGATGCTCAGCTGACCACCTCCTGGTGCCCAGGCCGCATGCTGGAGGAAGCAGGTGGCAAAT TCTGTGTGTGAGGCTCACATGTTCAATTCATGTGGGGCTGGG |
mm4_intergenic_Gm13282|Klhl9/Gm26566_chr4_88708672 |
CAGTGTTGCTCAGGTGTTTGGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTCA CCTGTAAAGTGTTATCCTT |
mm4_exon_Adam12_chr7_134225247 |
GCCGTTCTCATTACCACGGAACAAATTAGCGTCTCCCCCGCACCCCGCCTTCCTGCGGCGTCCCGCGGGTCCTAGAGACC GCTCCGGGGTCCCCGACAGCCTGGGTCCTATCCACTC |
mm4_exon_Ergic3_chr2_156008080 |
CTTTGAGGAGGCAGGTGAGGGGGTGGAGCCCCACTTACGCACCGCTACGCCTGCGCAAGACAGCGAGGGGCGTGACCCCG GGTCCAGGCCGGAAGCGGGAGGCTGGAGGGGCGGGCCGGCTGGTGTCCCCTTTCC |
mm4_intron_Fetub_chr16_22920126 |
AGCTTTCTTGCTGCGTCATGCGTGGGCGGGAGGCGGGAGGCAGGAGGAAGAACTCTGCCTAACAAATGTACTCCCCTGAC ATGGGCATGCCCCACCCCTGAGAACAGAGCCCTTGG |
mm4_intergenic_AU022754|Mirlet7c-2_chr15_85656265 |
GCCAGGCTCCCAACTTGTAAGATCCCACCCGAATTGGAAAGAAGGTGTCCTCCAAGGATGGGGGAGGAAGGCAGTCAGAG CTGTGAAGGCTGTAGAAGTCACCCTGGAGTGGGTGTATGTTTCAAAGGGAGCGGACTGAG |
mm4_intron_4931406P16Rik_chr7_34261863 |
GTGTGGGATCTGGCTGATGAAGAATGCAATGCTACTGGCAACTGCAGCGGCTCTGGAAACTGCCTCCTGCCTCCTGCCTC AGGCGGAACACTGATGAGACTATGTAGTTGAGTCTTCTCAGCCTCTTCTCCTATGGCA |
mm4_intergenic_Ppp1r14c|Iyd_chr10_3539440 |
AGCCACAAGGGAAGCTTTCTGTGAGTAATGGTTCTCTCTCCCATCCTTCCTTCCTCCCTCATCCCCAGCTGTGAGAACAA TACAAGCGAGG |
mm4_intergenic_Cd44|Pdhx_chr2_102916041 | AGAGCCAAGGCAGAGAGAGTTCTGAGAGGCTGGAGGTGGGAGGAAGGAGAAAAGCCTCTCCAAGTCCCCA |
mm4_intron_Ppard_chr17_28251709 |
GGAAGTTGTGGGTGAGGAGGTCCTGGTCCTTCTCTGGATGCTGGAGGGAGGTGGGGGAGGCTGGCTTTTAAAGGTCCCTC CAACAAAGACAGCACTAGTGGGGAAGAGGCTGT |
mm4_intergenic_Nim1|Zfp131_chr13_119760793 |
TGCAGCAATCCTGTTTTCACAAAGTTCTCCCTTGTCTCTCTTTTGACTCTCCCTTCTCCTTCCTCCCTCTTCCAGTGATA CCTGGAATGCTTTTCACATAATGTTATTTCTGACTGATGAGTTGGGGTGC |
mm4_intergenic_Sel1l3|Smim20_chr5_53259316 | AGCACAAACCAGACCCCAAAGAACCTCCGGGTGGGGGAGGGAGGGGGAGGGAGGGGCCCACCTGGGGAGGGAAGATCTC |
mm4_intron_Itpr1_chr6_108213944 |
CGGGGAAACAAGTGCTTTGGGATCTCAACACGTCCCTTACTTCCATCCACCTTCAATCCCCATCCGGCGTGTTCGAAGTT TCTGCGTGTCTGATCTTTCTGGCCAGCTACTGACG |
mm4_intron_Eno2_chr6_124766452 |
GGCTCAGGTCTCAAGTTCCCATCCTTTCACCTTAGCTGCCTGTTCACCTTCCTCCCTCATCCCTCACCTGGTTGCATGCT CCCTCCTCCCCTGGGAGAGGCTGGGCAAGTACAGTGCTCA |
mm4_intron_Rara_chr11_98969510 |
TGCAGAATCGACATGAGTGATCTCCAAATTATGCCAGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNGCGTCAGCAGCCGCCACGGAGGCCCTGTGAGTGATTG |
mm4_exon_Dscam_chr16_96709102 |
GAGTGAACTTCTGGGTGGCAGGCTCTAACCGGTGCCACCTACCTGCCTCATCCGCCGTGATGGTGATCTCGTTGCTGGGC TCACTCTTGCCAATCCGGT |
mm4_intron_Npas2_chr1_39330112 |
TCTTTTCCAGTGGGCTGCTCAGTCTCTCAGAGAGGATGCATGACACCCTTGCTCTCTCCTTCCTCCCGCATCGTAGGAAG AAGCTAGGTCTGGGAGAGCATGAGGACTGA |
mm4_exon_Lphn1_chr8_83900088 |
TAGTCCAGGCCAGGTTTTGCGCTTGTCCGCCGCCCCCCTCCCGGAGCCGGCGGGCCCGGCAGCGCCGAGAGCAGGCGGCT TGTCATTGCTGGTGC |
mm4_intergenic_Defb34|Spag11b_chr8_19128040 |
TGACTAGAGGCCCAGGAAAAAGCTTCTCTAATTCCTTGTCAATTTTAACACCAGTNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATCTGCCCTTCTCTTACTGCAC |
mm4_intergenic_Wdr66|Gm15857/Wdr66_chr5_123292265 |
GCGGAAGGTAGAATCTGGCACAGTGTTTAAAATCCCTGAGAACACATGGAGCTTCAGGAGGCTCTCCTTCCTCCCTCTTC AGGGGACACACTCTCCTGAGCAAAGGC |
mm4_intron_Nrtn_chr17_56755131 |
GGAAGGGAAACTGAGGCAGGCGCCGGATGCGGCAGTAACAGGGTGGGGAGGGAGGGCACTTGCCTGTCCTCTCCTTCCTT CCCAAATTCAAAGTTTGGTCTCAGGTCCTAGATAAAGGTGTCTGTTCTCCGAGCCTC |
mm4_intron_Il17rd_chr14_27039327 |
TCTCCGGGTTCCAGGTTACCTGGGGGCGCGCCGCGCGCACAGGTGTTCGCGCGCAGGCGAGCGGGGACCGCGGGGATCGC TGGACGCCGGATCCGCGAGCAACGGGGTTCTCAGCTTTTCTAGCCGCTCATCCTGC |
mm4_intergenic_Gm5250|Prdm14_chr1_13096001 |
TGATGCAGCTGGAAGATATGAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCAAAAGCAGAAAGAGGGCCC |