← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| mm0_exon_Pop5_chr5_115238112_F | TCGTCGGCAGCGTCTGCGGTTCAAGCACAGGTAA | 60.1 | 1.00 |
| mm0_exon_Pop5_chr5_115238112_R | GTCTCGTGGGCTCGGACCAGCTCGCACAGAAGGTA | 61.1 | 1.00 |
| mm4_intergenic_Snx16|Gm22795_chr3_10441494_F | TCGTCGGCAGCGTCCCAAGGTATCGTTAGACAAGATGC | 59.4 | 0.57 |
| mm4_intergenic_Snx16|Gm22795_chr3_10441494_R | GTCTCGTGGGCTCGGGAGCAATTCCTTAGGAGGGGA | 58.8 | 0.57 |
| mm4_intron_Fyn_chr10_39490617_F | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intron_Fyn_chr10_39490617_R | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intergenic_Lyz1|Cpsf6_chr10_117342219_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_Lyz1|Cpsf6_chr10_117342219_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_Oas1b|Oas1f_chr5_120840100_F | TCGTCGGCAGCGTCCATGTGGTACAGGCCCATGT | 60.0 | 0.25 |
| mm4_intergenic_Oas1b|Oas1f_chr5_120840100_R | GTCTCGTGGGCTCGGAGGAGCTAGCTCAAGGGAGG | 60.3 | 0.25 |
| mm4_exon_Rnaseh2a_chr8_84966137_F | TCGTCGGCAGCGTCTTTCCGCGAAAACTGCAGTG | 59.9 | 0.21 |
| mm4_exon_Rnaseh2a_chr8_84966137_R | GTCTCGTGGGCTCGGTCCCAGGAGAGAAGAGCAGA | 59.2 | 0.21 |
| mm4_intron_Sv2c_chr13_95995515_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Sv2c_chr13_95995515_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm3_exon_Phf7_chr14_31251145_F | TCGTCGGCAGCGTCCTCGCTCTCACGGAAGCTAC | 60.2 | 0.18 |
| mm3_exon_Phf7_chr14_31251145_R | GTCTCGTGGGCTCGGCGACTAAAGCTCCGGGACAG | 60.1 | 0.18 |
| mm4_exon_Adora2a_chr10_75333160_F | TCGTCGGCAGCGTCGCTGGAACAACTGCAGTCAG | 59.4 | 0.17 |
| mm4_exon_Adora2a_chr10_75333160_R | GTCTCGTGGGCTCGGGGGAGCAACACAAAAGCGAA | 59.6 | 0.17 |
| mm3_intron_Tpcn1_chr5_120552935_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intron_Tpcn1_chr5_120552935_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Rasal2/2810025M15Rik_chr1_157412353_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Rasal2/2810025M15Rik_chr1_157412353_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Rxfp2|Fry_chr5_150118822_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Rxfp2|Fry_chr5_150118822_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Nkain1|Pum1_chr4_130663287_F | TCGTCGGCAGCGTCCGAGTTCACACAGGCCCTG | 60.6 | 0.09 |
| mm4_intergenic_Nkain1|Pum1_chr4_130663287_R | GTCTCGTGGGCTCGGGGTGCATCATGGGAGCGG | 60.9 | 0.09 |
| mm4_intron_5930412G12Rik_chr5_128591010_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_5930412G12Rik_chr5_128591010_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Hecw2_chr1_54194577_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Hecw2_chr1_54194577_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Nupr1l_chr5_129908559_F | TCGTCGGCAGCGTCCAATAGGAGCACTGGGTGGG | 60.1 | 0.06 |
| mm4_exon_Nupr1l_chr5_129908559_R | GTCTCGTGGGCTCGGAGAAGGACGAGTTGGAGGGT | 60.1 | 0.06 |
| mm4_exon_Tnrc6c_chr11_117654968_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Tnrc6c_chr11_117654968_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Mpdu1_chr11_69657777_F | TCGTCGGCAGCGTCCAGGTACATTGGAGGCCTGG | 60.1 | 0.06 |
| mm4_exon_Mpdu1_chr11_69657777_R | GTCTCGTGGGCTCGGTTTCCTTGCCTGCTATGCCA | 59.9 | 0.06 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26572665_F | TCGTCGGCAGCGTCTCAAGCCACCTGATTAGCAG | 57.5 | 0.06 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26572665_R | GTCTCGTGGGCTCGGTCAGAGTGGAATCCTACTGGT | 57.5 | 0.06 |
| mm4_intergenic_Tox3|Chd9_chr8_90348398_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Tox3|Chd9_chr8_90348398_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Ptplb_chr16_35022704_F | TCGTCGGCAGCGTCGGAGCGAGCTCTGTGACC | 59.8 | 0.04 |
| mm4_intron_Ptplb_chr16_35022704_R | GTCTCGTGGGCTCGGCCTGACCAGGCAGCTCTG | 59.7 | 0.04 |
| mm3_exon_Plec_chr15_76176800_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_exon_Plec_chr15_76176800_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm10295|Mctp2_chr7_71386295_F | TCGTCGGCAGCGTCAGAGCAGCCCAGTGTTTGAA | 59.8 | 0.03 |
| mm4_intergenic_Gm10295|Mctp2_chr7_71386295_R | GTCTCGTGGGCTCGGACCCACAAACAGTTGGCTCA | 60.0 | 0.03 |
| mm4_exon_Setd5_chr6_113077407_F | TCGTCGGCAGCGTCGAATCCCCGTGCGGTCAG | 60.5 | 0.03 |
| mm4_exon_Setd5_chr6_113077407_R | GTCTCGTGGGCTCGGTAGGGCTGCGTGAGTCTCT | 60.0 | 0.03 |
| mm4_exon_Synj2_chr17_6005300_F | TCGTCGGCAGCGTCTGGAATTAGGGACACCGCTG | 59.7 | 0.03 |
| mm4_exon_Synj2_chr17_6005300_R | GTCTCGTGGGCTCGGTTGCTCCTTCAGAAGCACCA | 59.5 | 0.03 |
| mm4_intron_Trio_chr15_27840060_F | TCGTCGGCAGCGTCGCCTTCACAAATAAGAGCAGCC | 60.1 | 0.02 |
| mm4_intron_Trio_chr15_27840060_R | GTCTCGTGGGCTCGGCTGCGACCTGTGACTTCCTT | 59.9 | 0.02 |
| mm4_exon_Necab2_chr8_119446969_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Necab2_chr8_119446969_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_1700041M05Rik|Grik3_chr4_125425614_F | TCGTCGGCAGCGTCGAGTTTGTGTCTCCTGGCAGA | 59.9 | 0.01 |
| mm4_intergenic_1700041M05Rik|Grik3_chr4_125425614_R | GTCTCGTGGGCTCGGCCTGCATCCGAGGACATTGT | 60.1 | 0.01 |
| mm4_intron_Nrap_chr19_56366682_F | TCGTCGGCAGCGTCAGCCCACGGAGACTGAAAAG | 59.9 | 0.01 |
| mm4_intron_Nrap_chr19_56366682_R | GTCTCGTGGGCTCGGCCGTTGGCAATGTGAAGACA | 59.0 | 0.01 |
| mm4_intron_Trim13_chr14_61599611_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Trim13_chr14_61599611_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Add2|Tgfa_chr6_86151297_F | TCGTCGGCAGCGTCATGGCAGCTGGTGAGAAGAG | 59.7 | 0.01 |
| mm4_intergenic_Add2|Tgfa_chr6_86151297_R | GTCTCGTGGGCTCGGAATTGGGAGAGCCTCACACC | 59.6 | 0.01 |
| mm4_exon_Gm16278/Rab40c_chr17_25919119_F | TCGTCGGCAGCGTCAATCACATCGCAGCAGACCT | 59.7 | 0.01 |
| mm4_exon_Gm16278/Rab40c_chr17_25919119_R | GTCTCGTGGGCTCGGCCATTAGGGCCTGGAATGGG | 60.1 | 0.01 |
| mm4_exon_Mapk14_chr17_28691713_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Mapk14_chr17_28691713_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Mafb|Gm14221_chr2_160537466_F | TCGTCGGCAGCGTCCCCAGAAACATCCTGTCCCC | 60.0 | 0.00 |
| mm4_intergenic_Mafb|Gm14221_chr2_160537466_R | GTCTCGTGGGCTCGGCTGGGGTCTGGCTAAACAGC | 60.6 | 0.00 |
| mm4_intergenic_Trappc3|Col8a2_chr4_126280534_F | TCGTCGGCAGCGTCAGCTTCTGCCCATTGCTGTA | 59.6 | 0.00 |
| mm4_intergenic_Trappc3|Col8a2_chr4_126280534_R | GTCTCGTGGGCTCGGAAACACAGGCTCAGCATCCA | 59.8 | 0.00 |
| mm4_intron_Focad_chr4_88094701_F | TCGTCGGCAGCGTCGGCAGAAGTGAGGTAAGCCA | 59.6 | 0.00 |
| mm4_intron_Focad_chr4_88094701_R | GTCTCGTGGGCTCGGCAAGTGGTCCAGCTTCGGTC | 60.9 | 0.00 |
| mm4_intron_Gata2_chr6_88198555_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gata2_chr6_88198555_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Arhgef16_chr4_154290905_F | TCGTCGGCAGCGTCTGTGAACCCCAGAGCTCCTA | 59.8 | 0.00 |
| mm4_intron_Arhgef16_chr4_154290905_R | GTCTCGTGGGCTCGGCTCCCGGTATCCAGCCAAAA | 59.7 | 0.00 |
| mm4_intron_Msantd3_chr4_48540597_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Msantd3_chr4_48540597_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ipmk_chr10_71376110_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ipmk_chr10_71376110_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gpr146_chr5_139388827_F | TCGTCGGCAGCGTCTGGGACGGATGCTCTAGTGA | 60.0 | 0.00 |
| mm4_intron_Gpr146_chr5_139388827_R | GTCTCGTGGGCTCGGAGGCTTGCTATGGTGTTGCT | 59.9 | 0.00 |
| mm4_intron_Cxxc5_chr18_35831308_F | TCGTCGGCAGCGTCGCCGCGAGAGGAATGTGTTA | 60.4 | 0.00 |
| mm4_intron_Cxxc5_chr18_35831308_R | GTCTCGTGGGCTCGGAAACGGGGTCCCTCACTTTC | 59.8 | 0.00 |
| mm4_intron_Cit_chr5_115939252_F | TCGTCGGCAGCGTCGCGGGTTCCCTCAGAGTAAG | 59.8 | 0.00 |
| mm4_intron_Cit_chr5_115939252_R | GTCTCGTGGGCTCGGCTGAGGCAAGGAGAAAGGCA | 59.9 | 0.00 |
| mm4_intergenic_Gm6265|Nkx2-1_chr12_56521238_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm6265|Nkx2-1_chr12_56521238_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm16202|Ube2cbp_chr9_86330805_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm16202|Ube2cbp_chr9_86330805_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Zbtb4_chr11_69778412_F | TCGTCGGCAGCGTCAAGGCTGGAGGGGAAGATCA | 60.2 | 0.00 |
| mm4_exon_Zbtb4_chr11_69778412_R | GTCTCGTGGGCTCGGGTGGTGGTCTTCGTCCTCG | 60.0 | 0.00 |
| mm4_exon_Mn1_chr5_111419265_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Mn1_chr5_111419265_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Igsf8_chr1_172317530_F | TCGTCGGCAGCGTCGTCGGTGTCCTTTGGGAGAG | 60.0 | 0.00 |
| mm4_exon_Igsf8_chr1_172317530_R | GTCTCGTGGGCTCGGCGAAGCTCCCCAGAAGCTAG | 59.8 | 0.00 |
| mm4_exon_Atg13_chr2_91679342_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Atg13_chr2_91679342_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Alg9_chr9_50775619_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Alg9_chr9_50775619_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Alg9_chr9_50775589_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Alg9_chr9_50775589_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Aldh4a1/Gm21969_chr4_139642115_F | TCGTCGGCAGCGTCCAGAGTGTGGCGGGAAGAAT | 60.0 | 0.00 |
| mm4_exon_Aldh4a1/Gm21969_chr4_139642115_R | GTCTCGTGGGCTCGGAGCAGGCTGAGCACTTCTG | 60.0 | 0.00 |
| mm4_exon_Aes_chr10_81561196_F | TCGTCGGCAGCGTCCAGGAAGTGGGGCATGGG | 60.0 | 0.00 |
| mm4_exon_Aes_chr10_81561196_R | GTCTCGTGGGCTCGGTGAACTTGAGCTGCTGAGGG | 59.9 | 0.00 |
| mm4_exon_0610030E20Rik_chr6_72347694_F | TCGTCGGCAGCGTCCGCAAGCCAGACCTACAAGT | 60.3 | 0.00 |
| mm4_exon_0610030E20Rik_chr6_72347694_R | GTCTCGTGGGCTCGGCTGCTGTAAGGGCCAGGTG | 60.3 | 0.00 |
| mm3_intron_Ccdc69_chr11_55077805_F | TCGTCGGCAGCGTCCTACACAGTGACAGCCCCAC | 60.3 | 0.00 |
| mm3_intron_Ccdc69_chr11_55077805_R | GTCTCGTGGGCTCGGTCAGTCCCAGACAAACTGCA | 59.1 | 0.00 |
| mm3_exon_Plch2_chr4_154992969_F | TCGTCGGCAGCGTCGCTCATGAAGAGGCGGCTAT | 59.9 | 0.00 |
| mm3_exon_Plch2_chr4_154992969_R | GTCTCGTGGGCTCGGGGTTGAGGAAGACGTGGAGG | 60.0 | 0.00 |
| mm3_exon_Klhdc10_chr6_30402045_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_exon_Klhdc10_chr6_30402045_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm0_exon_Pop5_chr5_115238112 |
TGCGGTTCAAGCACAGGTAAGCAGCGCTGTCCCCGCGCCGTCCCCGCGACCCGGGCCGCGCTCACCCCGCTGTCGCCGCA GGTACCTTCTGTGCGAGCTGGT |
| mm4_intergenic_Snx16|Gm22795_chr3_10441494 |
CCAAGGTATCGTTAGACAAGATGCATAGCTTTTATCAGCCTGAGGTGATAGTGGGGTGAGCGAGAAGATGTAAAAACTAC AGGATAAAAGAAGCATTTGAAATGGAAAGATATATTATTTCCCCTCCTAAGGAATTGCTC |
| mm4_intergenic_Oas1b|Oas1f_chr5_120840100 |
CATGTGGTACAGGCCCATGTTGACCGGTGCCCTCAGGAGAGTTTCCTTTCTCCCAGGGCTTCTTGGGGTGCTCCTGAGGT GACAGCGGGGTGGGTTGCCTGAGTCACCTCCCTTGAGCTAGCTCCT |
| mm4_exon_Rnaseh2a_chr8_84966137 |
TTTCCGCGAAAACTGCAGTGGCTCTTGATGCTGACCAATCGGCTCGCAGGATCCGCCCTCGATGCGCCCTCCGGCGTGGT CGTTTACGCACGCGCTGCTGTCGCCGCTGGTCTGCTCTTCTCTCCTGGGA |
| mm3_exon_Phf7_chr14_31251145 |
CTCGCTCTCACGGAAGCTACGGGCCACCGCTTTCAGCCCAGGCTGCCCACCCAGCTGTCGCCGAGGGCGCCGCTGTCCCG GAGCTTTAGTCG |
| mm4_exon_Adora2a_chr10_75333160 |
GCTGGAACAACTGCAGTCAGAAAGATGAGAACTCCACAAAGACCTGCGGCGAGGGCCGGGTGACCTGTCTGTTTGAGGAC GTGGTACCCATGAATTACATGGTTTACTACAACTTCTTCGCTTTTGTGTTGCTCCC |
| mm4_intergenic_Nkain1|Pum1_chr4_130663287 |
CGAGTTCACACAGGCCCTGCCGTTGGCATGGCAACCGGGCAACCTGTTCCAGAGNNNNNNNNNNNNNNNNNNNNTCCGCA CACGCCGCTGCCACCGCCGGAGCTGGGGCCGGCGCCGGCGCCGCTCCCATGATGCACC |
| mm4_exon_Nupr1l_chr5_129908559 |
CAATAGGAGCACTGGGTGGGGCATTCCGGCGGAGTGACCAGCGGCGGCGGCGGGCGGAGCGCATCGGCGATGGACCCTCC AACTCGTCCTTCT |
| mm4_exon_Mpdu1_chr11_69657777 |
CAGGTACATTGGAGGCCTGGAGCAGAGTGACTACAGCCAGAGGCGTGAGCGGGGAGAGCAGCGCCAGCAGGACCATGGCA TAGCAGGCAAGGAAA |
| mm4_intergenic_Gm22227|Zfp703_chr8_26572665 |
TCAAGCCACCTGATTAGCAGGAGGCTCACCAGGCTGCCGCAGCTGGGATGGTAATTTATAGTAAACACTTTTTAACCAGT AGGATTCCACTCTGA |
| mm4_intron_Ptplb_chr16_35022704 |
GGAGCGAGCTCTGTGACCGGCGGGGTCGGACCCGCANNNNNNNNNNNNNNNNNNNNNAGCATGGGGCAGGCTGGCCGCAG AGCTGCCTGGTCAGG |
| mm4_intergenic_Gm10295|Mctp2_chr7_71386295 |
AGAGCAGCCCAGTGTTTGAAACAGATAAACCGGAGGCCACCAGGAATGCTGCTGTGGCCCACGCCGCTGCCGCTGCTGGC TTTGTGCAGGGGTGCATGGTAAACGGTACACCATTTGTGAGCCAACTGTTTGTGGGT |
| mm4_exon_Setd5_chr6_113077407 |
GAATCCCCGTGCGGTCAGTGGCGTTTCCGCTCGGGCAGCGGGCTGAGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NGGGGAGGGGCGGCCGCCGCCCGCCTGCGCTCAGAGACTCACGCAGCCCTA |
| mm4_exon_Synj2_chr17_6005300 |
TGGAATTAGGGACACCGCTGGTCCTAGACCTTCAGTGCTAAGATGGAGGTGCAGGTTGCCTGGCCCGGGTACCGTGGAGT CAGTGCTCACCCCGGTCTCGTTGCAGGCACATGGTGCTTCTGAAGGAGCAA |
| mm4_intron_Trio_chr15_27840060 |
GCCTTCACAAATAAGAGCAGCCTGAAGCCCAGCCCGCTGTCACGGCTGGGGAACCGGGGAACCAGAAGGCTGCCCCTTTC CTTGCAGTCTGCAAAGGCAGACAAATAAACAAGGAAGTCACAGGTCGCAG |
| mm4_intergenic_1700041M05Rik|Grik3_chr4_125425614 |
GAGTTTGTGTCTCCTGGCAGACCTCACCCAGCTGTGGACGCTGGCACTGTCCTGAGACTATGCATGTGTAAGACAATGTC CTCGGATGCAGG |
| mm4_intron_Nrap_chr19_56366682 |
AGCCCACGGAGACTGAAAAGTGATCCGTCCGTGGAAGGTTGGATAACACGGACAAGCAACACATTAGCAGAGACCAGCTC ACACCGCAGTGGACGCAGGAAACACACTGTCTTCACATTGCCAACGG |
| mm4_intergenic_Add2|Tgfa_chr6_86151297 |
ATGGCAGCTGGTGAGAAGAGGCTCCCCCAGCTGTGGCAGCAGGTGTGTGAGGTTCCCCAAGCTGTGGCAGCAGGTGTGTG AGGTTCCCCAAGCTGTGGCAGCAGGTGTGAGGCTCTCCCAATT |
| mm4_exon_Gm16278/Rab40c_chr17_25919119 |
AATCACATCGCAGCAGACCTATTTTCTCCCTGCACAGGGTGCGGTGTCTCTGAGCAAACCCCTCCGGCGGCAGTGCGGTG AGCACCGGGCCCGTCGTGCGGTATCTGCCGCGCGGCCCATTCCAGGCCCTAATGG |
| mm4_intergenic_Mafb|Gm14221_chr2_160537466 |
CCCAGAAACATCCTGTCCCCCGTCATTCCTTCATGCCAGGGTCTTTCTGCTCCAACAGATGGTTTCCTGTGGGCCGCCCC CGCAGCCACAGAGGGGAGAGCTGCTGTTTAGCCAGACCCCAG |
| mm4_intergenic_Trappc3|Col8a2_chr4_126280534 |
AGCTTCTGCCCATTGCTGTAAGCAGTGTAAGGGGAGGAGAAGCTGAAAGTGAGAGGTGAGTGGTCGCAGCTCTGCCCTCT GTGGCCGCTGGACATCTGTTGTGGATGCTGAGCCTGTGTTT |
| mm4_intron_Focad_chr4_88094701 |
GGCAGAAGTGAGGTAAGCCATGCGGCGTGGCTGACCGTCCACCGGCGGCAGCCGGGTGGGCAGTTGCCGCATCGGGTCCG GCTGCAGCCGCGGTCACGGGGGCGAGGGCGACCGAAGCTGGACCACTTG |
| mm4_intron_Arhgef16_chr4_154290905 |
TGTGAACCCCAGAGCTCCTAGACACACTGCCCACCCTGCTGTCGGGGCAGGTCCCTACCTTATTTTTGGCTGGATACCGG GAG |
| mm4_intron_Gpr146_chr5_139388827 |
TGGGACGGATGCTCTAGTGACAGTGTCAGACCTATGCCTCTGACGTGGTTGGTGCAGCCTACAAAGCCGCTCTGCTCTTG GGCTCTCCCCGCTGTGCCTGCTGGGAGGGGCATCTCCAAGAGCAACACCATAGCAAGCCT |
| mm4_intron_Cxxc5_chr18_35831308 |
GCCGCGAGAGGAATGTGTTAATTTTCTTACTGGGGGAAGTTGTCCTCGCCCGGAATGGGCTGTTTTCCGGCGGGGAGAGC GAGGTGCGCCTGGCTTTGTTTATGAATAGGTTGGACCGAAAGTGAGGGACCCCGTTT |
| mm4_intron_Cit_chr5_115939252 |
GCGGGTTCCCTCAGAGTAAGTAGTATCCGCCCGGGGTGGCTCTTCACCCACTCACCCCGCTGGCGGCGGCGGCTGTGTTT ATTGCCTTTCTCCTTGCCTCAG |
| mm4_exon_Zbtb4_chr11_69778412 |
AAGGCTGGAGGGGAAGATCAGCTTTGGAGACCCTACTATTCATACAAGCCTAAGCGCAAGGCTGGAGCTACTGCCGGCGG GGCCAGCGGGGTCAGTGGACTGCCCCGAGGACGAAGACCACCAC |
| mm4_exon_Igsf8_chr1_172317530 |
GTCGGTGTCCTTTGGGAGAGCCATTCCGGAGGCGCCAGTGGGGCGAGCCACTCTACAGGAAGTAGTGGGACTGCACTCCG ACATGGCGGTGGAGGCTGGAGCGCCCTACGCTGAGAGGCTAGCTTCTGGGGAGCTTCG |
| mm4_exon_Aldh4a1/Gm21969_chr4_139642115 |
CAGAGTGTGGCGGGAAGAATTTCCATTTTGTGCACAGCTCGGCCGACGTGGACAGCGTGGTGAGCGGGACGCTGCGCTCG GCATTCGAGTACGGCGGCCAGAAGTGCTCAGCCTGCT |
| mm4_exon_Aes_chr10_81561196 |
CAGGAAGTGGGGCATGGGCGGGGCAGGACGCCGCTCACCCTCCTGCCCCCGCAGGGCTCCTCCCACCTCCCTCAGCAGCT CAAGTTCA |
| mm4_exon_0610030E20Rik_chr6_72347694 |
CGCAAGCCAGACCTACAAGTGTACCTGCCGCGCCACCGAGGTGAGCCGCCGCCTGTNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNAAGCCCCTCTCTCTGCGTCAGGGCTCCTGCCCACCTGGCCCTTACAGCAG |
| mm3_intron_Ccdc69_chr11_55077805 |
CTACACAGTGACAGCCCCACACTGAAGGCCACCTGCCACGGACACACCAGTCACCTCCCCGCGGGGACAGCAGGGTCAGC TGCAGTTTGTCTGGGACTGA |
| mm3_exon_Plch2_chr4_154992969 | GCTCATGAAGAGGCGGCTATTCTGCCTGCTCACCCCGCTGTCTTCCCCGGCCTCCACGTCTTCCTCAACC |