← return to the list of all guides

Contents:

PCR primers for off-targets of GAAGGTACCTGCGGCGACAG CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
mm0_exon_Pop5_chr5_115238119_F TCGTCGGCAGCGTCTGCGGTTCAAGCACAGGTAA 60.1 1.00
mm0_exon_Pop5_chr5_115238119_R GTCTCGTGGGCTCGGGTCGTCCAGGCTCAAACGG 61.0 1.00
mm4_exon_Lpar1_chr4_58437626_F Primer3: not found at this Tm N.d. 0.44
mm4_exon_Lpar1_chr4_58437626_R Primer3: not found at this Tm N.d. 0.44
mm4_intergenic_Phyhipl|4930533K18Rik_chr10_70837536_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Phyhipl|4930533K18Rik_chr10_70837536_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Mut|Gm6771_chr17_41199559_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Mut|Gm6771_chr17_41199559_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm6291|Epc1_chr18_6387499_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm6291|Epc1_chr18_6387499_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm23981|Ttc29_chr8_78081523_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm23981|Ttc29_chr8_78081523_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm12832|Cyp4a31_chr4_115561411_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm12832|Cyp4a31_chr4_115561411_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Ccdc114|Abcc6_chr7_45962299_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Ccdc114|Abcc6_chr7_45962299_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Atp6v1h|Oprk1_chr1_5228063_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Atp6v1h|Oprk1_chr1_5228063_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Adamts16|8030423J24Rik_chr13_70880200_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Adamts16|8030423J24Rik_chr13_70880200_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_1700025G04Rik|Tsen15_chr1_152182553_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_1700025G04Rik|Tsen15_chr1_152182553_R Primer3: not found at this Tm N.d. 0.40
mm4_intron_Abca12_chr1_71363103_F Primer3: not found at this Tm N.d. 0.39
mm4_intron_Abca12_chr1_71363103_R Primer3: not found at this Tm N.d. 0.39
mm4_intergenic_Otoa|Hs3st2_chr7_121285880_F TCGTCGGCAGCGTCCAGACCTTTCAGCAAGTGCA 58.6 0.30
mm4_intergenic_Otoa|Hs3st2_chr7_121285880_R GTCTCGTGGGCTCGGCAAACTCCCATGGTCTGCCT 59.9 0.30
mm4_intergenic_Zbtb7b|Gm15417/Zbtb7b_chr3_89389479_F Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Zbtb7b|Gm15417/Zbtb7b_chr3_89389479_R Primer3: not found at this Tm N.d. 0.29
mm4_intron_Vwa5b2_chr16_20589660_F TCGTCGGCAGCGTCCTGCAGTGATGGGGAGACTG 60.1 0.17
mm4_intron_Vwa5b2_chr16_20589660_R GTCTCGTGGGCTCGGGGAAACCATTTAGGCCTCGC 59.2 0.17
mm4_exon_Slc30a3_chr5_31093470_F TCGTCGGCAGCGTCTCCAAGGACCACTCGGACTC 60.8 0.16
mm4_exon_Slc30a3_chr5_31093470_R GTCTCGTGGGCTCGGGCTCTGCAGTTCGGACTCC 60.4 0.16
mm4_exon_Whsc1_chr5_33820809_F Primer3: not found at this Tm N.d. 0.12
mm4_exon_Whsc1_chr5_33820809_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Ttc28|Mir701_chr5_110979401_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Ttc28|Mir701_chr5_110979401_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Fam19a5_chr15_87668534_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Fam19a5_chr15_87668534_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_A930001A20Rik|Gm9733_chr3_15055669_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_A930001A20Rik|Gm9733_chr3_15055669_R Primer3: not found at this Tm N.d. 0.09
mm3_intron_Nedd9_chr13_41446417_F TCGTCGGCAGCGTCAAAGGAAAGAGCCAGCACGG 60.8 0.08
mm3_intron_Nedd9_chr13_41446417_R GTCTCGTGGGCTCGGGGGTGGGGCAAGAAACCTTA 59.8 0.08
mm4_intergenic_1700017N19Rik|Gm26278_chr10_100865702_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_1700017N19Rik|Gm26278_chr10_100865702_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Ccnd1|Tpcn2_chr7_145098385_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Ccnd1|Tpcn2_chr7_145098385_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Mrvi1_chr7_110872734_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Mrvi1_chr7_110872734_R Primer3: not found at this Tm N.d. 0.05
mm4_exon_Grm4_chr17_27472942_F Primer3: not found at this Tm N.d. 0.05
mm4_exon_Grm4_chr17_27472942_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Ccnl1|Veph1_chr3_65985852_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Ccnl1|Veph1_chr3_65985852_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Bcas3_chr11_85600610_F TCGTCGGCAGCGTCGAATGTTCCCACTTTATGTAACTGA 57.3 0.04
mm4_intron_Bcas3_chr11_85600610_R GTCTCGTGGGCTCGGAAGGTTTCCTGAAGCTGCCA 59.8 0.04
mm4_intergenic_Kctd1|Aqp4_chr18_15171359_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Kctd1|Aqp4_chr18_15171359_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Tdrd9_chr12_112013316_F TCGTCGGCAGCGTCCCCCTAGGTCTGGGTGAGAT 59.7 0.03
mm4_exon_Tdrd9_chr12_112013316_R GTCTCGTGGGCTCGGGCACAAAGTTCTGAGTGGCAG 60.0 0.03
mm4_intergenic_Ctnnd2|Gm26416_chr15_31185135_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Ctnnd2|Gm26416_chr15_31185135_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Reln_chr5_22033416_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Reln_chr5_22033416_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Egr2_chr10_67539267_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Egr2_chr10_67539267_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_Ccdc125_chr13_100693704_F TCGTCGGCAGCGTCACCTGTGTAAAAGAGCGGCA 59.8 0.03
mm4_exon_Ccdc125_chr13_100693704_R GTCTCGTGGGCTCGGTCCCAAAGCAACCAAAAGCC 59.5 0.03
mm4_exon_Lrdd_chr7_141439439_F Primer3: not found at this Tm N.d. 0.02
mm4_exon_Lrdd_chr7_141439439_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25519|Gm5548_chr3_112421641_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25519|Gm5548_chr3_112421641_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Slc25a37|RP24-67I21.6/Slc25a37_chr14_69276812_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Slc25a37|RP24-67I21.6/Slc25a37_chr14_69276812_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm21464|RP24-175I14.9/Gm21464_chr14_69495062_F TCGTCGGCAGCGTCCTCACCAGGCGAGGACATTT 60.0 0.02
mm4_intergenic_Gm21464|RP24-175I14.9/Gm21464_chr14_69495062_R GTCTCGTGGGCTCGGCCCAGGGCACATGGCTTAA 60.0 0.02
mm4_intron_Steap3_chr1_120260414_F TCGTCGGCAGCGTCCTGTGCATCACCCCTAGTCC 59.8 0.02
mm4_intron_Steap3_chr1_120260414_R GTCTCGTGGGCTCGGCTGCTCAGAGCTTTGGGTGA 59.9 0.02
mm4_intergenic_mmu-mir-6393|Zdhhc25_chr15_88487257_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_mmu-mir-6393|Zdhhc25_chr15_88487257_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Calcb_chr7_114722106_F Primer3: not found at this Tm N.d. 0.02
mm4_exon_Calcb_chr7_114722106_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Tmem52_chr4_155469446_F TCGTCGGCAGCGTCAGAACTTGGACCGTGCTGG 59.9 0.02
mm4_exon_Tmem52_chr4_155469446_R GTCTCGTGGGCTCGGCCACTTACCCCACATGCCAT 60.0 0.02
mm4_intergenic_Rpl21-ps10|Gm25574_chr3_38189484_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Rpl21-ps10|Gm25574_chr3_38189484_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Prkce_chr17_86249067_F TCGTCGGCAGCGTCTGGTCAAGAGCATTGGGAGG 59.6 0.02
mm4_intron_Prkce_chr17_86249067_R GTCTCGTGGGCTCGGCAGAGCTAACACACGGGGAG 60.1 0.02
mm4_intergenic_Zfp868|Zfp964_chr8_69648113_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Zfp868|Zfp964_chr8_69648113_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm8032|Gm17204_chr14_42571696_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm8032|Gm17204_chr14_42571696_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22593|Mettl4_chr17_94670949_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22593|Mettl4_chr17_94670949_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm17165|Gm17038_chr14_42231917_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm17165|Gm17038_chr14_42231917_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13248|Gm21411_chr4_146821132_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13248|Gm21411_chr4_146821132_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13150|C230088H06Rik_chr4_146998217_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13150|C230088H06Rik_chr4_146998217_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13051|Gm26573_chr4_146133292_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13051|Gm26573_chr4_146133292_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_2610305D13Rik|Gm16211_chr4_147666732_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_2610305D13Rik|Gm16211_chr4_147666732_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Slc44a1_chr4_53529071_F TCGTCGGCAGCGTCCTCGGGGCTCTGGTGTTTTA 59.6 0.01
mm4_intron_Slc44a1_chr4_53529071_R GTCTCGTGGGCTCGGCCGTTGGTGCTACTAGGATCA 59.2 0.01
mm4_intergenic_Gm25382|Cited2_chr10_17125925_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm25382|Cited2_chr10_17125925_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13322|Nebl_chr2_17219386_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13322|Nebl_chr2_17219386_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Dock2_chr11_34732321_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Dock2_chr11_34732321_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Fancd2_chr6_113538218_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Fancd2_chr6_113538218_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Fam78a_chr2_32078550_F TCGTCGGCAGCGTCCCGGGTGTCTGTCTGTTCAT 59.6 0.00
mm4_intron_Fam78a_chr2_32078550_R GTCTCGTGGGCTCGGTTGGGCTAGCTGTGACCAAG 59.9 0.00
mm4_intron_Vstm4_chr14_32919390_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Vstm4_chr14_32919390_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Asb1|Twist2_chr1_91625117_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Asb1|Twist2_chr1_91625117_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Tmem63a_chr1_180965815_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Tmem63a_chr1_180965815_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Magi2_chr5_20442620_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Magi2_chr5_20442620_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ccnd1|Tpcn2_chr7_145035192_F TCGTCGGCAGCGTCGCCACTCAGTGACACGAGAG 60.3 0.00
mm4_intergenic_Ccnd1|Tpcn2_chr7_145035192_R GTCTCGTGGGCTCGGAGAGGCTGAAAAGGTTGGCA 59.8 0.00
mm4_intergenic_Gm22805|Gm4791_chr9_46643238_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22805|Gm4791_chr9_46643238_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Col23a1_chr11_51483766_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Col23a1_chr11_51483766_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ppapdc1a_chr7_129325511_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ppapdc1a_chr7_129325511_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nr1i2_chr16_38277734_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nr1i2_chr16_38277734_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Adcy8_chr15_64867653_F TCGTCGGCAGCGTCCAGGTCAGTGGATGAGTTTGGA 59.9 0.00
mm4_intron_Adcy8_chr15_64867653_R GTCTCGTGGGCTCGGCAGCCATCAGCCCCTAGATG 59.9 0.00
mm4_intergenic_Wdr96|Gsto1_chr19_47840015_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Wdr96|Gsto1_chr19_47840015_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tgfbr2|Rbms3_chr9_116457592_F TCGTCGGCAGCGTCCGAACCAGAGCCTGTGTGAA 60.2 0.00
mm4_intergenic_Tgfbr2|Rbms3_chr9_116457592_R GTCTCGTGGGCTCGGCTAAAGACTGGCTTCTTGCGC 59.8 0.00
mm4_exon_Serpine3_chr14_62689098_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Serpine3_chr14_62689098_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Cabin1_chr10_75746530_F TCGTCGGCAGCGTCCCACAAAGTCTGCTCACCCA 60.1 0.00
mm4_exon_Cabin1_chr10_75746530_R GTCTCGTGGGCTCGGCAATCCAACTGTTGCCGTGG 60.0 0.00
mm3_intron_Scamp5_chr9_57457393_F TCGTCGGCAGCGTCAGCTGTGGGAATCAGGACAC 59.6 0.00
mm3_intron_Scamp5_chr9_57457393_R GTCTCGTGGGCTCGGCTCCTCACTCCTTTGCCCAG 60.0 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm0_exon_Pop5_chr5_115238119 TGCGGTTCAAGCACAGGTAAGCAGCGCTGTCCCCGCGCCGTCCCCGCGACCCGGGCCGCGCTCACCCCGCTGTCGCCGCA
GGTACCTTC
TGTGCGAGCTGGTGTCCGAGGACGCGCGCTGCCGTTTGAGCCTGGACGAC
mm4_intergenic_Otoa|Hs3st2_chr7_121285880 CAGACCTTTCAGCAAGTGCATGAACCATGAAGCATAGCTTTTCTAACACCAACAAGGCCTGAATTAAAAGCAAAGAGGTA
CCTGCCGCAACAGAGG
AAGAAAGAAGGCAGACCATGGGAGTTTG
mm4_intron_Vwa5b2_chr16_20589660 CTGCAGTGATGGGGAGACTGGATTGAGGGCAACCGCTTTGGTGAGCACACAAACGAAGGCACATGCCGCGAAAGGGGAGG
TTTCTGCAATAACCTATTTGCTTCTTGTGGGGGGCGAGGCCTAAATGGTTTCC
mm4_exon_Slc30a3_chr5_31093470 TCCAAGGACCACTCGGACTCCCCGCTGGGCCACCAGTGTGGAGGGAACTGCGGCGACTGTGGCGCTCGGAGTCCGAACTG
CAGAGC
mm3_intron_Nedd9_chr13_41446417 AAAGGAAAGAGCCAGCACGGCAGCAGAAGCACCCGGACAAGGTGCCTGCGGCGGCAGAGGAGGCTTTGCCTTGGGGCAGT
TTCCCTCCTAGGTAAGGTTTCTTGCCCCACCC
mm4_intron_Bcas3_chr11_85600610 GAATGTTCCCACTTTATGTAACTGAATAAAGGAAAACATTTAGTGAGAAGGAAGGTCCCTGTGGCAGCAGTGGTGGCAGC
TTCAGGAAACCTT
mm4_exon_Tdrd9_chr12_112013316 CCCCTAGGTCTGGGTGAGATCAATTACATGCATGAGCTCCTCACAAACATGATTCACAAAAGGTACGTNNNNNNNNNNNN
N
NNNNNNNNNNNNNNNNNNCCCTTTGTCTGCCACTCAGAACTTTGTGC
mm4_exon_Ccdc125_chr13_100693704 ACCTGTGTAAAAGAGCGGCAACGTGGATCAACAGACAACACCAGGCAGACGATGGTACCTGGGGCGCAAGTGGCAAGAAT
GGGGGCTTTTGGTTGCTTTGGGA
mm4_intergenic_Gm21464|RP24-175I14.9/Gm21464_chr14_69495062 CTCACCAGGCGAGGACATTTTTTTCTTCCTGTAACCCAGTAAGATGGATCTGGGGTTAATGCCCCCCAGTCTGCTGGGAA
GGGACCTGAAGTGACAGAGG
TTAAGCCATGTGCCCTGGG
mm4_intron_Steap3_chr1_120260414 CTGTGCATCACCCCTAGTCCCTCATTCTGAGCTGTGCTCCCTTGATGCTGGAAGATGCCTGCGGAGGCAGTGGGGTTCAA
GGAGGACTCTCCATCACCAGTGAGCCCCAGAGTCACCCAAAGCTCTGAGCAG
mm4_exon_Tmem52_chr4_155469446 AGAACTTGGACCGTGCTGGGAAAGGCTGGAGCGGTGGAGTGAGCTAGCAGATACCTCACCTGCTTCAGGTGCCGCTGTCC
CCGCAGGTGCCCGC
CCCAGGCCCGCTGGAGCAGCCTATGGCATGTGGGGTAAGTGG
mm4_intron_Prkce_chr17_86249067 TGGTCAAGAGCATTGGGAGGGGCTGAAGGTGCGTGAGGTGACAGGGGTTGATTCTTGGTGACACAAGAGGGATTCTTGGC
TCCCCGTGTGTTAGCTCTG
mm4_intron_Slc44a1_chr4_53529071 CTCGGGGCTCTGGTGTTTTATTAATTTTACAGTTTTTAAGTATATAAAACTCTGAATCATTGTCCAGTCTACAACAAAGT
TGGGTCCAATTCCCACTGTCCCCACAGGTGCCTTGAGGTTGATCCTAGTAGCACCAACGG
mm4_intron_Fam78a_chr2_32078550 CCGGGTGTCTGTCTGTTCATCCCCAGGCCACTTGTTCCCCTCTCTCCGAAGGTAGCTTCATGCTGGAGCCACCACTTGGT
CACAGCTAGCCCAA
mm4_intergenic_Ccnd1|Tpcn2_chr7_145035192 GCCACTCAGTGACACGAGAGTCACACCCCTGGCCCAGCAGGTACCTTGCTCTTTAAAGTCTTGCCTCCAGAGCCTTTGCG
AACTCCTGTCTGAAATGCCAACCTTTTCAGCCTCT
mm4_intron_Adcy8_chr15_64867653 CAGGTCAGTGGATGAGTTTGGACAGTGTTTCTTCAAGGTTCTGTATGCTTGTAGTTTAGCTGTGACCCAACCACTGTAGA
CGCAGGTACATTT
GGAGCTATCTGTGACCTTCTTTATTCATCTAGGGGCTGATGGCTG
mm4_intergenic_Tgfbr2|Rbms3_chr9_116457592 CGAACCAGAGCCTGTGTGAACCACAGGAGCTGCAGGTACCTTCAAAAAATAAATGATGGGTACAAGCAAATGCCATAGGG
CGCAAGAAGCCAGTCTTTAG
mm4_exon_Cabin1_chr10_75746530 CCACAAAGTCTGCTCACCCATAGGAGTGCCTGTGTCCAGCAGGCTGGGACTGTGCAGTGGGAAGCTAGCTGCGGTCACAG
AGG
TGTAGGAGAGCACAGGCTCAGCCACGGCAACAGTTGGATTG
mm3_intron_Scamp5_chr9_57457393 AGCTGTGGGAATCAGGACACCTCTAAGGTACCTGCTGCCACAGAGGAAGCAGTTCCAGATCTGAATACAGGAAGGTCAGA
TAGGAGTTAGCTGGGCAAAGGAGTGAGGAG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_ozJbN0W08qlgEBn3RvZK.txt --name MY_EXPERIMENT

Version 5.01 - Documentation  - Contact us - Downloads/local installation - Citation - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.