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Contents:

PCR primers for off-targets of CCGCGCCGTCCCCGCGACCC GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
mm0_exon_Pop5_chr5_115238085_F TCGTCGGCAGCGTCTGCGGTTCAAGCACAGGTAA 60.1 1.00
mm0_exon_Pop5_chr5_115238085_R GTCTCGTGGGCTCGGCTCGCACAGAAGGTACCTGC 60.7 1.00
mm3_exon_Reps1_chr10_18055895_F Primer3: not found at this Tm N.d. 0.39
mm3_exon_Reps1_chr10_18055895_R Primer3: not found at this Tm N.d. 0.39
mm4_exon_Atp6v0a1_chr11_101055098_F TCGTCGGCAGCGTCAGGCCATCCACACCATTGAG 60.0 0.26
mm4_exon_Atp6v0a1_chr11_101055098_R GTCTCGTGGGCTCGGAGAGAGAGCACACTCACGTG 59.4 0.26
mm4_intergenic_Tbx2|Gm11444_chr11_85842147_F Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Tbx2|Gm11444_chr11_85842147_R Primer3: not found at this Tm N.d. 0.24
mm4_intron_Ptma_chr1_86527266_F Primer3: not found at this Tm N.d. 0.22
mm4_intron_Ptma_chr1_86527266_R Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Banp|Gm22_chr8_122172637_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Banp|Gm22_chr8_122172637_R Primer3: not found at this Tm N.d. 0.17
mm3_exon_Peli1_chr11_21091606_F TCGTCGGCAGCGTCCTGGTGCCTGCGTTCAAC 59.3 0.16
mm3_exon_Peli1_chr11_21091606_R GTCTCGTGGGCTCGGCTCACCGGACGACTGCTG 60.1 0.16
mm4_intron_Csmd2_chr4_128476228_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Csmd2_chr4_128476228_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Prr14|Fbrs_chr7_127479114_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Prr14|Fbrs_chr7_127479114_R Primer3: not found at this Tm N.d. 0.14
mm2_intron_Sctr_chr1_120007340_F TCGTCGGCAGCGTCCTGCTGCTCATCAATGCAGC 60.2 0.13
mm2_intron_Sctr_chr1_120007340_R GTCTCGTGGGCTCGGGAAGAGAGATCGGCTGCTCC 59.9 0.13
mm3_exon_Brd3_chr2_27475364_F Primer3: not found at this Tm N.d. 0.13
mm3_exon_Brd3_chr2_27475364_R Primer3: not found at this Tm N.d. 0.13
mm3_intron_Eef2_chr10_81177010_F TCGTCGGCAGCGTCGAGGGGTTGTGCTTGGTGAT 60.2 0.12
mm3_intron_Eef2_chr10_81177010_R GTCTCGTGGGCTCGGGAGGTGTCCAAGGAAGCCAG 60.3 0.12
mm4_intergenic_Cdkn1b|RP23-45G16.5_chr6_134927115_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Cdkn1b|RP23-45G16.5_chr6_134927115_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Fryl_chr5_73255904_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Fryl_chr5_73255904_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Camta1_chr4_151672537_F TCGTCGGCAGCGTCCAATAATCTTCGCCGCGGC 60.0 0.10
mm4_intron_Camta1_chr4_151672537_R GTCTCGTGGGCTCGGCTCCAGACAGCTTGAGGGTC 59.7 0.10
mm4_intergenic_Cgnl1|RP23-79K5.1_chr9_71771656_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Cgnl1|RP23-79K5.1_chr9_71771656_R Primer3: not found at this Tm N.d. 0.10
mm4_exon_Sms_chrX_157492100_F TCGTCGGCAGCGTCCCAGGAGTTAGGAGGTGGGA 59.9 0.08
mm4_exon_Sms_chrX_157492100_R GTCTCGTGGGCTCGGCGTCCCTGCTTCCTCCTCTA 60.3 0.08
mm3_intron_Gm4425_chr12_22156174_F TCGTCGGCAGCGTCGAAACTTTTCCCGGGTGTGG 59.3 0.08
mm3_intron_Gm4425_chr12_22156174_R GTCTCGTGGGCTCGGGAGCGGTAGAGTAGCCCGAG 61.5 0.08
mm3_intron_Gm20472_chr12_23314886_F Primer3: not found at this Tm N.d. 0.08
mm3_intron_Gm20472_chr12_23314886_R Primer3: not found at this Tm N.d. 0.08
mm3_intron_Gm10479_chr12_20416097_F TCGTCGGCAGCGTCATCGAGGAGGTGAGCGGTAG 60.8 0.08
mm3_intron_Gm10479_chr12_20416097_R GTCTCGTGGGCTCGGGAAGCCTAACAGGTGCCCAG 60.6 0.08
mm3_intergenic_Hpcal1|B430203G13Rik_chr12_17882356_F TCGTCGGCAGCGTCATCGAGGAGGTGAGCGGTAG 60.8 0.08
mm3_intergenic_Hpcal1|B430203G13Rik_chr12_17882356_R GTCTCGTGGGCTCGGGCACTGGGTATCGGGCAC 60.5 0.08
mm3_intergenic_Gm5633|Gm22908_chr12_21898770_F TCGTCGGCAGCGTCGAAGTGTACCGGGTGCCC 60.0 0.08
mm3_intergenic_Gm5633|Gm22908_chr12_21898770_R GTCTCGTGGGCTCGGCCTCTAGCTTCACCACGCG 60.5 0.08
mm3_intergenic_Gm5633|Gm22908_chr12_21784085_F TCGTCGGCAGCGTCCTGGGTATCAGGCACCAGG 59.4 0.08
mm3_intergenic_Gm5633|Gm22908_chr12_21784085_R GTCTCGTGGGCTCGGCATTGAGGAGGTGAGCGGTA 59.1 0.08
mm3_intergenic_Gm5633|Gm22908_chr12_21778419_F Primer3: not found at this Tm N.d. 0.08
mm3_intergenic_Gm5633|Gm22908_chr12_21778419_R Primer3: not found at this Tm N.d. 0.08
mm3_intergenic_Gm3993|Gm10479_chr12_20197076_F TCGTCGGCAGCGTCATCGAGGAGGTGAGCGGTAG 60.8 0.08
mm3_intergenic_Gm3993|Gm10479_chr12_20197076_R GTCTCGTGGGCTCGGGACACTGGGTATCGGGCAC 60.1 0.08
mm3_intergenic_Gm23913|Gm10480_chr12_18136345_F TCGTCGGCAGCGTCATCGAGGAGGTGAGCGGTAG 60.8 0.08
mm3_intergenic_Gm23913|Gm10480_chr12_18136345_R GTCTCGTGGGCTCGGGAGGGGACTGGGTATCGGG 60.8 0.08
mm3_intergenic_Gm23913|Gm10480_chr12_17993292_F TCGTCGGCAGCGTCATCGAGGAGGTGAGCGGTAG 60.8 0.08
mm3_intergenic_Gm23913|Gm10480_chr12_17993292_R GTCTCGTGGGCTCGGGCACTGGGTATCGGGCAC 60.5 0.08
mm3_intergenic_Gm17391|2410018L13Rik_chr12_22905980_F Primer3: not found at this Tm N.d. 0.08
mm3_intergenic_Gm17391|2410018L13Rik_chr12_22905980_R Primer3: not found at this Tm N.d. 0.08
mm3_intergenic_Gm10479|1700030C10Rik_chr12_20615463_F TCGTCGGCAGCGTCATCGAGGAGGTGAGCGGTAG 60.8 0.08
mm3_intergenic_Gm10479|1700030C10Rik_chr12_20615463_R GTCTCGTGGGCTCGGCGGAGAGCACTGGGTATCG 59.6 0.08
mm3_intergenic_5730507C01Rik|Gm3944_chr12_18610544_F TCGTCGGCAGCGTCATCGAGGAGGTGAGCGGTAG 60.8 0.08
mm3_intergenic_5730507C01Rik|Gm3944_chr12_18610544_R GTCTCGTGGGCTCGGGCACTGGGTATCGTGCACTA 59.8 0.08
mm3_exon_Gm9312_chr12_24251998_F Primer3: not found at this Tm N.d. 0.08
mm3_exon_Gm9312_chr12_24251998_R Primer3: not found at this Tm N.d. 0.08
mm3_exon_Gm5784_chr12_19388247_F TCGTCGGCAGCGTCATCGAGGAGGTGAGCGGTAG 60.8 0.08
mm3_exon_Gm5784_chr12_19388247_R GTCTCGTGGGCTCGGGCTGGAGAGCACTGGGTATC 59.8 0.08
mm3_exon_Gm3944_chr12_18854031_F TCGTCGGCAGCGTCATCGAGGAGGTGAGCGGTAG 60.8 0.08
mm3_exon_Gm3944_chr12_18854031_R GTCTCGTGGGCTCGGCACTGGGTATCCGGCACC 59.8 0.08
mm4_exon_A230072E10Rik/Fam120c_chrX_151344475_F TCGTCGGCAGCGTCCGCTGCCTTTGGTGCTTAC 59.7 0.08
mm4_exon_A230072E10Rik/Fam120c_chrX_151344475_R GTCTCGTGGGCTCGGAATGGTGAGCAGGGTGATGG 60.0 0.08
mm4_intergenic_BC052040|Meis2_chr2_115859176_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_BC052040|Meis2_chr2_115859176_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Gm15608_chr10_82178505_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Gm15608_chr10_82178505_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Ppp3r1|Wdr92/Ppp3r1_chr11_17159798_F TCGTCGGCAGCGTCCTCCCCGAGATTCGCCGC 62.9 0.07
mm4_intergenic_Ppp3r1|Wdr92/Ppp3r1_chr11_17159798_R GTCTCGTGGGCTCGGAAAAGGCCGAACGAGCCAG 60.9 0.07
mm3_intron_Pmp22_chr11_63132535_F Primer3: not found at this Tm N.d. 0.07
mm3_intron_Pmp22_chr11_63132535_R Primer3: not found at this Tm N.d. 0.07
mm4_exon_Pdgfb_chr15_80014044_F Primer3: not found at this Tm N.d. 0.07
mm4_exon_Pdgfb_chr15_80014044_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Rbpj_chr5_53602769_F TCGTCGGCAGCGTCCATCCATGCCCACCATCTGT 60.1 0.07
mm4_intron_Rbpj_chr5_53602769_R GTCTCGTGGGCTCGGAGAGACATAAAGACAGCAGACAGT 59.4 0.07
mm4_exon_Ccdc85b_chr19_5456861_F Primer3: not found at this Tm N.d. 0.07
mm4_exon_Ccdc85b_chr19_5456861_R Primer3: not found at this Tm N.d. 0.07
mm4_exon_Fam211a_chr11_62648020_F TCGTCGGCAGCGTCGACAGGTCAGCCTTCCTCAC 60.0 0.06
mm4_exon_Fam211a_chr11_62648020_R GTCTCGTGGGCTCGGGGGTGACAGGAGAGCGCC 62.4 0.06
mm4_intergenic_1700128E19Rik|Arhgef7_chr8_11727860_F TCGTCGGCAGCGTCCCGCCCTACCCACAAAGG 60.0 0.06
mm4_intergenic_1700128E19Rik|Arhgef7_chr8_11727860_R GTCTCGTGGGCTCGGTCACCGGAGAAGAAAGACGC 60.0 0.06
mm3_exon_Drd4_chr7_141294210_F TCGTCGGCAGCGTCTTTACTGGGCCACTTTCCGC 60.8 0.06
mm3_exon_Drd4_chr7_141294210_R GTCTCGTGGGCTCGGGCAGTCTGGGAAGAAGGGAC 60.0 0.06
mm3_intergenic_Chrdl2|Pold3_chr7_100042276_F TCGTCGGCAGCGTCACGGGCTTTGATTCGTGACT 59.9 0.06
mm3_intergenic_Chrdl2|Pold3_chr7_100042276_R GTCTCGTGGGCTCGGAACAAACACGTCCCACAAGC 59.5 0.06
mm4_intergenic_Phf10|Gm3417_chr17_14961581_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Phf10|Gm3417_chr17_14961581_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm3435|Tcte3_chr17_15024532_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm3435|Tcte3_chr17_15024532_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_9030025P20Rik|Gm3448_chr17_14993164_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_9030025P20Rik|Gm3448_chr17_14993164_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Sgip1_chr4_102759397_F Primer3: not found at this Tm N.d. 0.06
mm4_exon_Sgip1_chr4_102759397_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Leprotl1_chr8_34146656_F TCGTCGGCAGCGTCGTTGCCCCGTTCTGGTCC 60.6 0.06
mm4_intron_Leprotl1_chr8_34146656_R GTCTCGTGGGCTCGGATGGCGGGCATCAAAGGTG 61.0 0.06
mm4_exon_Tlk1_chr2_70825520_F Primer3: not found at this Tm N.d. 0.06
mm4_exon_Tlk1_chr2_70825520_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Gclc_chr9_77754636_F Primer3: not found at this Tm N.d. 0.05
mm4_exon_Gclc_chr9_77754636_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_C330006A16Rik|Gm13553_chr2_26179177_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_C330006A16Rik|Gm13553_chr2_26179177_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Nhlrc2|Adrb1_chr19_56722279_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Nhlrc2|Adrb1_chr19_56722279_R Primer3: not found at this Tm N.d. 0.05
mm4_exon_Ptgds_chr2_25469102_F Primer3: not found at this Tm N.d. 0.04
mm4_exon_Ptgds_chr2_25469102_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Lmtk2_chr5_144100581_F TCGTCGGCAGCGTCAAGAGACGTGTGTCCCGAAG 59.6 0.04
mm4_exon_Lmtk2_chr5_144100581_R GTCTCGTGGGCTCGGCTTCAGTCAGTCGGTCAGCC 60.3 0.04
mm4_intergenic_Cacng4|Gm11650_chr11_107794593_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Cacng4|Gm11650_chr11_107794593_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Igf2bp3_chr6_49214416_F TCGTCGGCAGCGTCCCGGGATCTTAGCGTCCTTG 60.2 0.04
mm4_exon_Igf2bp3_chr6_49214416_R GTCTCGTGGGCTCGGTCGGGTTTTAAAACGACCCCT 59.8 0.04
mm4_exon_Slc45a4_chr15_73645506_F Primer3: not found at this Tm N.d. 0.04
mm4_exon_Slc45a4_chr15_73645506_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Stat5b_chr11_100850271_F TCGTCGGCAGCGTCCTCTCGTAGCCACCACCAG 59.4 0.04
mm4_intron_Stat5b_chr11_100850271_R GTCTCGTGGGCTCGGCTGTCTAGAAGCTGGGCAGG 59.8 0.04
mm4_intron_Htt_chr5_34762131_F TCGTCGGCAGCGTCCAGAGGAACCGCTGCACC 60.7 0.04
mm4_intron_Htt_chr5_34762131_R GTCTCGTGGGCTCGGTGGACAGGGAACAGTGTTGG 59.8 0.04
mm4_intergenic_Camk2n1|Vwa5b1_chr4_138462806_F TCGTCGGCAGCGTCGATGTTCACGGGCTGCTGTA 60.3 0.03
mm4_intergenic_Camk2n1|Vwa5b1_chr4_138462806_R GTCTCGTGGGCTCGGGCTTTGCACAGAGATTGCCC 60.1 0.03
mm4_intron_Sh3bp4_chr1_89070771_F TCGTCGGCAGCGTCTTCCCTGCCCTCTGTGGT 60.1 0.03
mm4_intron_Sh3bp4_chr1_89070771_R GTCTCGTGGGCTCGGCGCTCTCTTCGCTCCTTCTC 60.2 0.03
mm4_exon_Zfp664_chr5_124863026_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_Zfp664_chr5_124863026_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_Smyd2_chr1_189922087_F TCGTCGGCAGCGTCAAGCTAGAACGGACACGCG 60.4 0.02
mm4_exon_Smyd2_chr1_189922087_R GTCTCGTGGGCTCGGACTGCGAGTGCTGCTTCG 60.7 0.02
mm4_intergenic_Gm15754|Med13l_chr5_118560681_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm15754|Med13l_chr5_118560681_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Rala_chr13_17943812_F TCGTCGGCAGCGTCCTTGAAAGCGAGGGAGGTCG 60.7 0.02
mm4_intron_Rala_chr13_17943812_R GTCTCGTGGGCTCGGAAGGTCACCTGTATAGCGCC 59.5 0.02
mm4_intergenic_Gm25748|Creb1_chr1_64532413_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25748|Creb1_chr1_64532413_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Gm10687/Mcam_chr9_44134551_F Primer3: not found at this Tm N.d. 0.02
mm4_exon_Gm10687/Mcam_chr9_44134551_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Cd63_chr10_128910154_F TCGTCGGCAGCGTCGGCGGAGATCTACCTGGAGT 60.4 0.02
mm4_intron_Cd63_chr10_128910154_R GTCTCGTGGGCTCGGTCAGACCCACGTCTCCCAG 60.6 0.02
mm4_intergenic_Elmod1|Tnfaip8l3_chr9_53975358_F TCGTCGGCAGCGTCGTTTCCCTGGGAGCAAGACG 60.9 0.02
mm4_intergenic_Elmod1|Tnfaip8l3_chr9_53975358_R GTCTCGTGGGCTCGGGGCCAAGTGCGCAGTTTTAA 59.9 0.02
mm2_exon_Sbk1_chr7_126273071_F TCGTCGGCAGCGTCGGGCAGACGAAGATCGACAC 60.8 0.02
mm2_exon_Sbk1_chr7_126273071_R GTCTCGTGGGCTCGGCTGCAGGACTCTAGGGACCT 60.0 0.02
mm4_exon_Ptms_chr6_124917631_F Primer3: not found at this Tm N.d. 0.02
mm4_exon_Ptms_chr6_124917631_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Evx2|Hoxd13_chr2_74667870_F TCGTCGGCAGCGTCGGGTGGCTGCAAAGTCAACT 61.1 0.02
mm4_intergenic_Evx2|Hoxd13_chr2_74667870_R GTCTCGTGGGCTCGGCACCCCTCTTCAGCCCTTTG 60.6 0.02
mm4_intergenic_Egr1|Etf1_chr18_34887366_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Egr1|Etf1_chr18_34887366_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Fbxo11|Gm4832_chr17_88065366_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fbxo11|Gm4832_chr17_88065366_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Wdr20a_chr12_110738295_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Wdr20a_chr12_110738295_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Slc2a1_chr4_119109249_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Slc2a1_chr4_119109249_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ephx4_chr5_107403458_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ephx4_chr5_107403458_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Zfand4|March8_chr6_116337931_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Zfand4|March8_chr6_116337931_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Kmt2b_chr7_30588557_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Kmt2b_chr7_30588557_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Ephb2_chr4_136835893_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Ephb2_chr4_136835893_R Primer3: not found at this Tm N.d. 0.01
mm3_exon_Itga5_chr15_103366674_F Primer3: not found at this Tm N.d. 0.01
mm3_exon_Itga5_chr15_103366674_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Rab31_chr17_65729985_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Rab31_chr17_65729985_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Gm10657/Cd276_chr9_58555036_F TCGTCGGCAGCGTCACAAAGGTGGACCCAGCAG 59.8 0.01
mm4_exon_Gm10657/Cd276_chr9_58555036_R GTCTCGTGGGCTCGGCAACAGTGGCGGCGATTG 59.8 0.01
mm4_intergenic_Il13|Rps2-ps4_chr11_53636361_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Il13|Rps2-ps4_chr11_53636361_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Arhgef16_chr4_154299870_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Arhgef16_chr4_154299870_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24720|Tshz1_chr18_83936519_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24720|Tshz1_chr18_83936519_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pou4f1|Rnf219_chr14_104468133_F TCGTCGGCAGCGTCGCAGCTGTCTCCGTCTGTC 60.4 0.00
mm4_intergenic_Pou4f1|Rnf219_chr14_104468133_R GTCTCGTGGGCTCGGCTATAAGAGCGGCTCCCACG 60.0 0.00
mm4_exon_Srf_chr17_46555680_F TCGTCGGCAGCGTCCAGACGACTCTGGCCGAG 59.5 0.00
mm4_exon_Srf_chr17_46555680_R GTCTCGTGGGCTCGGCAACCTGAACCGGACCCC 59.9 0.00
mm4_intron_Tardbp_chr4_148626594_F TCGTCGGCAGCGTCCTTAGGCCTTAACGACGCGG 61.1 0.00
mm4_intron_Tardbp_chr4_148626594_R GTCTCGTGGGCTCGGTCGAGTGTTGCTTCGGAGAG 59.7 0.00
mm4_intergenic_Grin2b/Gm26653|Grin2b_chr6_136173328_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Grin2b/Gm26653|Grin2b_chr6_136173328_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ldb1|Ldb1/Pprc1_chr19_46039698_F TCGTCGGCAGCGTCGAGGGTGGAGCTGCCCTG 62.1 0.00
mm4_intergenic_Ldb1|Ldb1/Pprc1_chr19_46039698_R GTCTCGTGGGCTCGGCGGATCCCTTCAATCCCCAG 59.8 0.00
mm4_exon_Snx18_chr13_113617785_F TCGTCGGCAGCGTCGACGAAGGTGGAGAAGCGAT 59.8 0.00
mm4_exon_Snx18_chr13_113617785_R GTCTCGTGGGCTCGGCGCTCGGACCTGTCTCTG 59.5 0.00
mm4_exon_Asmt_chrX_170673668_F TCGTCGGCAGCGTCGATGACCGCCATGACTGACC 60.8 0.00
mm4_exon_Asmt_chrX_170673668_R GTCTCGTGGGCTCGGCTCAGGCCCGACGACCTC 61.8 0.00
mm4_exon_Tmem229b_chr12_79007222_F TCGTCGGCAGCGTCCGACTATTCCCGCATCCAGT 59.6 0.00
mm4_exon_Tmem229b_chr12_79007222_R GTCTCGTGGGCTCGGTTTGCACCGCATCCGTACTA 59.7 0.00
mm4_exon_Rc3h2_chr2_37422801_F TCGTCGGCAGCGTCGGTCGCTAAGGGCCGCTC 62.8 0.00
mm4_exon_Rc3h2_chr2_37422801_R GTCTCGTGGGCTCGGAGTTTGAGGCCCTGAAACCG 60.5 0.00
mm4_intron_Vrk1_chr12_106010516_F TCGTCGGCAGCGTCGGAAGCCCGGTTCTAGAAGG 59.8 0.00
mm4_intron_Vrk1_chr12_106010516_R GTCTCGTGGGCTCGGCCAGTGCTAAGGAAGTGGCA 59.9 0.00
mm4_intron_Heca_chr10_17947417_F TCGTCGGCAGCGTCGAGCACTTCCTGACTCGCAT 60.1 0.00
mm4_intron_Heca_chr10_17947417_R GTCTCGTGGGCTCGGCGGGAGACGCTAAGAATGGT 59.5 0.00
mm4_intergenic_n-R5s168|Plekha5_chr6_140423989_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_n-R5s168|Plekha5_chr6_140423989_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pax3|Sgpp2_chr1_78217311_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pax3|Sgpp2_chr1_78217311_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gcc1|Arf5_chr6_28423494_F TCGTCGGCAGCGTCTTCAGTTCTTCCGCAAACGC 59.6 0.00
mm4_intergenic_Gcc1|Arf5_chr6_28423494_R GTCTCGTGGGCTCGGGCAACAGCAGAAGGAGGAGG 60.6 0.00
mm4_intergenic_Ckap4|C330046G13Rik_chr10_84534051_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ckap4|C330046G13Rik_chr10_84534051_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Scarf2_chr16_17797394_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Scarf2_chr16_17797394_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Hoxa4_chr6_52191582_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Hoxa4_chr6_52191582_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Gm26776/Ccdc3_chr2_5137874_F TCGTCGGCAGCGTCTAGCCGTGCACCCAGCTC 62.0 0.00
mm4_exon_Gm26776/Ccdc3_chr2_5137874_R GTCTCGTGGGCTCGGCTACGCTCTAGCCCGGGC 61.9 0.00
mm4_exon_Gm2155/6330419J24Rik_chrX_56374906_F TCGTCGGCAGCGTCTCAGTGATGCTCAACGTCCG 60.3 0.00
mm4_exon_Gm2155/6330419J24Rik_chrX_56374906_R GTCTCGTGGGCTCGGGTCTGGAGGCACTGTGCG 60.7 0.00
mm4_exon_Glud1_chr14_34310932_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Glud1_chr14_34310932_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_F3_chr3_121723592_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_F3_chr3_121723592_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Cpeb2/Gm7854_chr5_43234703_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Cpeb2/Gm7854_chr5_43234703_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Ccdc84_chr9_44417759_F TCGTCGGCAGCGTCGATCGTGCTCGGGGACATAG 60.0 0.00
mm4_exon_Ccdc84_chr9_44417759_R GTCTCGTGGGCTCGGTCCTAGCTGGCTTCTGTCCT 59.9 0.00
mm4_exon_AW822252_chrX_53716480_F TCGTCGGCAGCGTCCAGTCTGGAGGCACTGTGC 60.6 0.00
mm4_exon_AW822252_chrX_53716480_R GTCTCGTGGGCTCGGTGCGGATCGAGTGAGCAAAC 61.0 0.00
mm3_exon_Yy1_chr12_108793966_F Primer3: not found at this Tm N.d. 0.00
mm3_exon_Yy1_chr12_108793966_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm0_exon_Pop5_chr5_115238085 TGCGGTTCAAGCACAGGTAAGCAGCGCTGTCCCCGCGCCGTCCCCGCGACCCGGGCCGCGCTCACCCCGCTGTCGCCGCA
GGTACCTTCTGTGCGAG
mm4_exon_Atp6v0a1_chr11_101055098 AGGCCATCCACACCATTGAGTACTGCTTGGGTTGTATCTCCAACACCGCGTCGTACCTGCGACTCTGGGCCCTCAGCCTG
GCCCACGCACGTGAGTGTGCTCTCTCT
mm3_exon_Peli1_chr11_21091606 CTGGTGCCTGCGTTCAACCGGTGCTNNNNNNNNNNNNNNNNNNNNNNTGTCCGTGCGGCGGGCCGAGCTGGCGTTCCGGC
CACCAGCAGTCGTCCGGTGAG
mm2_intron_Sctr_chr1_120007340 CTGCTGCTCATCAATGCAGCGCACCCTGTGAGTACCCCGCCCCGTCCCCGCGCCCCCGGATGTGGACCCCACGGACCAGT
CCACGGTCCTCTATCCGGCCCTAGGAGTTACGGGGAGCAGCCGATCTCTCTTC
mm3_intron_Eef2_chr10_81177010 GAGGGGTTGTGCTTGGTGATGTGGCAGCGGGCAAAGCGCCGTCCCCGCGCCCCTGGTGACGGGCGGAGGGTGTCCTCGGG
AGGTGACAGCCTGTAGGGCTGGCTTCCTTGGACACCTC
mm4_intron_Camta1_chr4_151672537 CAATAATCTTCGCCGCGGCAAAGCATAAGCTCTGTCTAGATCCAAGTGGGTTTCACGCCGCCTGGGGCGCGGGTACAGTG
CGG
TTGTCACTTGACCCTCAAGCTGTCTGGAG
mm4_exon_Sms_chrX_157492100 CCAGGAGTTAGGAGGTGGGAGGGGCCGCGGGCAAGCGANNNNNNNNNNNNNNNNNNNNNNNACCCGCCTCTTAGAGGAGG
AAGCAGGGACG
mm3_intron_Gm4425_chr12_22156174 GAAACTTTTCCCGGGTGTGGGCGCAGGCGGAGGGCACTGGGTATCCGGCACCAGGCGCGGAAGTGTACCGGGTGCCCAGG
GCGCAGCGCGATCCCCGCGACCCTAGTCTCCCGCCCGGCTCGGGCTACTCTACCGCTC
mm3_intron_Gm10479_chr12_20416097 ATCGAGGAGGTGAGCGGTAGCGTAGCCCGCGCCGGGCAGGAGGCTAGGGTCGCGGGGATCGCGCTGCGCCCTGGGCACCT
GTTAGGCTTC
mm3_intergenic_Hpcal1|B430203G13Rik_chr12_17882356 ATCGAGGAGGTGAGCGGTAGCGTCGCCCGCGCGGGGCGGGAGGCTAGGGTCGCGGGGATCGCGCTGCGCCCTGGGGACCC
GCTAGGCTTCCGCGCCTGGTGCCCGATACCCAGTGC
mm3_intergenic_Gm5633|Gm22908_chr12_21898770 GAAGTGTACCGGGTGCCCAGGGCGCAGCGCGATCCCCGCGACCCTAGCCTCCCGCCCGGCGCGGGCTACGCTACCGCTCA
CCTCCCCGATGGCCGCCACGGTGTTCCGGCACTGGGCCGTGCGCGTGGTGAAGCTAGAGG
mm3_intergenic_Gm5633|Gm22908_chr12_21784085 CTGGGTATCAGGCACCAGGCGCGGAAGCCTACCGGGTGCCCAGGGCGCAGCGCGATCCCCGCGACCCTAGCCTCCTGCCC
GGCGCGGGCTACGCTACCGCTCACCTCCTCAATG
mm3_intergenic_Gm3993|Gm10479_chr12_20197076 ATCGAGGAGGTGAGCGGTAGCATAGCCCGCGCCCTGCGGGAGGCTAGGGTCGCGGGGATCGCGCTGCGCCCTGGCCACCC
GCTAGGCTTCCGCGCCTGGTGCCCGATACCCAGTGTC
mm3_intergenic_Gm23913|Gm10480_chr12_18136345 ATCGAGGAGGTGAGCGGTAGCGTCGCCCGCGGGGGGCGGGAGGCTAGGGTCGCGGGGATCGCGCTGCGCCCTGGGCACCC
GGTAGGCTTCCGCGCTTGGTGCCCGATACCCAGTCCCCTC
mm3_intergenic_Gm23913|Gm10480_chr12_17993292 ATCGAGGAGGTGAGCGGTAGCATAGCCCGCGCCGGGCAGGAGGCTAGGGTCGCGGGGATCGCGCTGCGCCCTGGGCACCC
GGTAGGCTTCCGCGCTTGGTGCCCGATACCCAGTGC
mm3_intergenic_Gm10479|1700030C10Rik_chr12_20615463 ATCGAGGAGGTGAGCGGTAGCTTAGCCGGCGCCGGGCGGGAGGCTAGGGTCGCGGGGATCGCGCTGCGCCCTGGGCACCC
GCTAGGCTTCCGCGCCTGGTGCCCGATACCCAGTGCTCTCCG
mm3_intergenic_5730507C01Rik|Gm3944_chr12_18610544 ATCGAGGAGGTGAGCGGTAGCGTAGCCCGCGCCGGGCGGGAGGCTAGGGTCGCGGGGATCGCGCTGCGCCCTGGGCACCT
GGTAGGCTTCCGCGCCTAGTGCACGATACCCAGTGC
mm3_exon_Gm5784_chr12_19388247 ATCGAGGAGGTGAGCGGTAGCTTAGCCGGCGCCGGGCGGGAGGCTAGGGTCGCGGGGATCGCGCTGCGCCCTGGGCACCC
GCTAGGCTTCCGCGCCTGGTGGCCGATACCCAGTGCTCTCCAGC
mm3_exon_Gm3944_chr12_18854031 ATCGAGGAGGTGAGCGGTAGCGTAGCCCGCGCCGGGCGGGAGGCTAGGGTCGCGGGGATCGCGCTGCGCCCTGGGCACCC
GGTAGGCTTCCGCGCCTGGTGCCGGATACCCAGTG
mm4_exon_A230072E10Rik/Fam120c_chrX_151344475 CGCTGCCTTTGGTGCTTACTCCGGGGGCGCGGGGCCGTCTCGGCACCATCACCCTGCTCACCATT
mm4_intergenic_Ppp3r1|Wdr92/Ppp3r1_chr11_17159798 CTCCCCGAGATTCGCCGCCCCCGCCCTCCGCACGACCCTGGGCCTCCGGGCGCCCACAGAGCCGCCGCCCCCGGGCTCCT
AAGCCCGTGGGCCTCCGCGCAACCCCGGCCCCGTCGCCTGTCTGGCTCGTTCGGCCTTTT
mm4_intron_Rbpj_chr5_53602769 CATCCATGCCCACCATCTGTAAGCTCCCTACTGCATGCATCCATGTTCACCATCTGTATGCTTCACACCTGCTTCTNNNN
NNNNNNNNNNNNNNNNNNNACGTCCCTGGGAAGCACTGTCTGCTGTCTTTATGTCTCT
mm4_exon_Fam211a_chr11_62648020 GACAGGTCAGCCTTCCTCACCCTAGCCTGAGGCCCCAAATTCAGCAGGAAACACAGAGGCACCTGCTGACACACCGCTCC
ATCCCCGCGAAACGGG
GACCCTGGGCGGTGCCTCCCGGCGCTCTCCTGTCACCC
mm4_intergenic_1700128E19Rik|Arhgef7_chr8_11727860 CCGCCCTACCCACAAAGGCGCGCAGCCCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCGCGTCTTTCTTCTCCGGTGA
mm3_exon_Drd4_chr7_141294210 TTTACTGGGCCACTTTCCGCGGCCTGCGGCGCTGGGAGGCAGCCCGGCACACCAAACTTCACANNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNN
NNNNNNNNTGTCGGACCCTACTCAGGGTCCCTTCTTCCCAGACTGC
mm3_intergenic_Chrdl2|Pold3_chr7_100042276 ACGGGCTTTGATTCGTGACTCCGGCGTGGACCCAGACGCTAATTAAAATCGCCTNNNNNNNNNNNNNNNNNNNNNNNNNA
CAAAGCCCGCTTTGACCTGGCGGCACAGGCCAGGCTTGTGGGACGTGTTTGTT
mm4_intron_Leprotl1_chr8_34146656 GTTGCCCCGTTCTGGTCCAGGGGCGGAGCGCCACCGCCGGAGGAGCCCGGCTGGGTCGTCCCCACGCCGCCCCGCCCCAT
CCCTACGGCCCCGGCCCCGCGGCCCAGGCCCACCTTTGATGCCCGCCAT
mm4_exon_Lmtk2_chr5_144100581 AAGAGACGTGTGTCCCGAAGCAGCCGGCGCCGGTGTCGCTGAGGCGGCGCGGAGGGCTGACCGACTGACTGAAG
mm4_exon_Igf2bp3_chr6_49214416 CCGGGATCTTAGCGTCCTTGAAGACACTTTCCAGGTCCGCGGGGCCGGCGTGGTCGCTGAGGTTCCCGATGTACAATTTG
TTCATTGTGAAAAGGGGTCGTTTTAAAACCCGA
mm4_intron_Stat5b_chr11_100850271 CTCTCGTAGCCACCACCAGCGCCCCGGGCCCCTCTGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAG
CGATGGCGGCGGCTCGGCCCTGCCCAGCTTCTAGACAG
mm4_intron_Htt_chr5_34762131 CAGAGGAACCGCTGCACCGACCGTGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGCCTGCGTGCTGGG
CATGGCCAACACTGTTCCCTGTCCA
mm4_intergenic_Camk2n1|Vwa5b1_chr4_138462806 GATGTTCACGGGCTGCTGTACTGGCCTGGGTCGCTGAGACGGTGGGGGTCCATCAGGGTCAAAGAATTCCCTCCTTTTCT
TGTGTGCACACTGCGTCTTCGGGGGAGGGGGGCAATCTCTGTGCAAAGC
mm4_intron_Sh3bp4_chr1_89070771 TTCCCTGCCCTCTGTGGTCACAGCCCGACCTGGGCTGCGGATCGTGGTCGCTANNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNTTCTGAGAAGGAGCGAAGAGAGCG
mm4_exon_Smyd2_chr1_189922087 AAGCTAGAACGGACACGCGTGGGGCGGCGCAGGTCCGGCTGCGGGGCAGGCCGTGGCTCAGCGGACGCGGCGCACGTGGC
TCCGCGTGGCCCCTCCCGCCCGCACGGTCCCCGCGTACCTGGCGAAGCAGCACTCGCAGT
mm4_intron_Rala_chr13_17943812 CTTGAAAGCGAGGGAGGTCGGGGTGGAGTGCGGCGCAGGGACTNNNNNNNNNNNNNNNNNNNNCCCAGGTCGCGGGCACC
GCCCGG
GCACAGCCCTGGGAGCCACCGGCGCTATACAGGTGACCTT
mm4_intron_Cd63_chr10_128910154 GGCGGAGATCTACCTGGAGTCCGGCCGCGCCCTCCGTGCGTCCCGGGATCCCGAGATCCCGAGGCCCCGCTGCGTGGCGC
GCCCCAGTCCGGCCGGCGAGCGGGGAGGGCTGGGAGACGTGGGTCTGA
mm4_intergenic_Elmod1|Tnfaip8l3_chr9_53975358 GTTTCCCTGGGAGCAAGACGCTGGCGGCTACTGAGCATGCTCAGTCGCGGAANNNNNNNNNNNNNNNNNNNNNNNNAGGA
GTGAGCTCAGGCCCCCGCGCCAGAATGTGGGAAGAAAGGGTTAAAACTGCGCACTTGGCC
mm2_exon_Sbk1_chr7_126273071 GGGCAGACGAAGATCGACACGGCGCCCAGGCCCCCTGCCGCGGCGTCCCCGCGGCCCCAGCCCAGGTAAGTGGGACCCAG
GTGAGGGGCGGCAGGTGAGTGGTACCGAGGTCCCTAGAGTCCTGCAG
mm4_intergenic_Evx2|Hoxd13_chr2_74667870 GGGTGGCTGCAAAGTCAACTCCTGGGTCTGGGGGAGGGCGGGGATGCGAGGTGGGCCTCCCAAAGGGCTGAAGAGGGGTG
mm4_exon_Gm10657/Cd276_chr9_58555036 ACAAAGGTGGACCCAGCAGCCCACGCCGCCCCGCGCCACGCAAGCACTCACCGCGCCGCGCCGACTCCCGGGACTCGGGG
ACGCGGCGG
GGCGCAGCCCAATCGCCGCCACTGTTG
mm4_intergenic_Pou4f1|Rnf219_chr14_104468133 GCAGCTGTCTCCGTCTGTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCTTNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNCTCGTCGAGCCGCGACCGGCGGCGGCGCGTGGGAGCCGCTCTTATAG
mm4_exon_Srf_chr17_46555680 CAGACGACTCTGGCCGAGCCCCGCGCCGTTCCCGGGGACCCGGCCCCCGTTCGCCCCGCGAGTNNNNNNNNNNNNNNNNN
NNNNNNNNNTCGGGGTCCGGTTCAGGTTG
mm4_intron_Tardbp_chr4_148626594 CTTAGGCCTTAACGACGCGGCTGCGCGAGGCCAACCAGGCCCCGCTCTACCGCTCCCTCCCCGCGGCGCAGGCCGCACCG
GGCCTCTCCGAAGCAACACTCGA
mm4_intergenic_Ldb1|Ldb1/Pprc1_chr19_46039698 GAGGGTGGAGCTGCCCTGCCGGCCCCGCCCCCTCCCCGCGAGCGAGGAGGAAGAGCGGGCGGAGGGAGGGAGCGAGGAGG
GACTGCTGAGAAAAACAAAAGAGATTGAGACCGGGGGACTGGGGATTGAAGGGATCCG
mm4_exon_Snx18_chr13_113617785 GACGAAGGTGGAGAAGCGATTGAGGTTGCGGCTCACCGTGGCCGAGCTCTTGGCTCCAGACGCTTGGTGCGCCGGGGGCG
CCGAGACGCCGCGG
GAGCCCAGAGACAGGTCCGAGCG
mm4_exon_Asmt_chrX_170673668 GATGACCGCCATGACTGACCCGTGACCCCAGGTGCTGTTCGCGGGCTGCGCGCTCCGCGTGTTCGACGCCGCGGCCCTGG
GCCCCGTGGACGCCGCGG
CGCTGGCGAGGTCGTCGGGCCTGAG
mm4_exon_Tmem229b_chr12_79007222 CGACTATTCCCGCATCCAGTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGCTGCAGCCGCTGCTGCCCCACGGA
ACCCAGCCCCAAGCGGCGGCGGCCGCACTCACCCATAGTACGGATGCGGTGCAAA
mm4_exon_Rc3h2_chr2_37422801 GGTCGCTAAGGGCCGCTCCCGGGGGCCCCGCGACGGCGCGGCTCGGCGACGGAGGCGCCTCGTCTCGCCGGGGCAGAGCT
CGGCGGCGGTTTCAGGGCCTCAAACT
mm4_intron_Vrk1_chr12_106010516 GGAAGCCCGGTTCTAGAAGGCCGCCCGAAGCCTAGCCGGGACCAGGGTGGNNNNNNNNNNNNNNNNNNNNNNNNNCTGGA
CCGGCCGAGCCTGCCACTTCCTTAGCACTGG
mm4_intron_Heca_chr10_17947417 GAGCACTTCCTGACTCGCATTGTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGGTG
AGGAAGTTGGCGCTCGCCCCGCCGCTGTCGTCTTACCATTCTTAGCGTCTCCCG
mm4_intergenic_Gcc1|Arf5_chr6_28423494 TTCAGTTCTTCCGCAAACGCGCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGTGACGTCGGGCGGAC
CCGGACGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTCCTCCTTCTGCTGTTGC
mm4_exon_Gm26776/Ccdc3_chr2_5137874 TAGCCGTGCACCCAGCTCTCCGGAGCGCGTGCAGGCGAGCCGAGCGCCCCGTCCGCGGTTCTCGGGCAGGCGCTGCGGGC
TCCCCGGCTCCCCGCCGTCCCGGGCACCCGGGCGGGCCATGCGCCCGGGCTAGAGCGTAG
mm4_exon_Gm2155/6330419J24Rik_chrX_56374906 TCAGTGATGCTCAACGTCCGCCTGCAGGTGCGGATCGAGTGAGCAAACGCCCTCCGCCACGGAGGAGAGGGCCATCCGCG
CCTTCCGCGCCATCCTGG
CGGCCGCAGGAAGGACAGCGGGGCGCACAGTGCCTCCAGAC
mm4_exon_Ccdc84_chr9_44417759 GATCGTGCTCGGGGACATAGCGCTCCACCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCACCTGCAGGACAGAAGCC
AGCTAGGA
mm4_exon_AW822252_chrX_53716480 CAGTCTGGAGGCACTGTGCGCCCCGCTGTCCTTCCTGCGGCCGCCAGGATGGCGCGGAAGGCGCGGATGGCCCTCTCCGC
CGTGGCGGAGGGCGTTTGCTCACTCGATCCGCA

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_ozJbN0W08qlgEBn3RvZK.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.