← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| mm0_exon_Pop5_chr5_115238006_F | Primer3: not found at this Tm | N.d. | 1.00 |
| mm0_exon_Pop5_chr5_115238006_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm3_intergenic_Insig1|En2_chr5_28086436_F | Primer3: not found at this Tm | N.d. | 0.85 |
| mm3_intergenic_Insig1|En2_chr5_28086436_R | Primer3: not found at this Tm | N.d. | 0.85 |
| mm4_intergenic_Gap43|4932412D23Rik_chr16_42590837_F | Primer3: not found at this Tm | N.d. | 0.57 |
| mm4_intergenic_Gap43|4932412D23Rik_chr16_42590837_R | Primer3: not found at this Tm | N.d. | 0.57 |
| mm4_intergenic_Hs3st2|Usp31_chr7_121626990_F | Primer3: not found at this Tm | N.d. | 0.45 |
| mm4_intergenic_Hs3st2|Usp31_chr7_121626990_R | Primer3: not found at this Tm | N.d. | 0.45 |
| mm3_intergenic_Tbx5|n-R5s176_chr5_119893785_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm3_intergenic_Tbx5|n-R5s176_chr5_119893785_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm3_intergenic_Enpp4|Clic5_chr17_44139874_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm3_intergenic_Enpp4|Clic5_chr17_44139874_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intron_Cntnap3_chr13_64750041_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intron_Cntnap3_chr13_64750041_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intron_Zfp423_chr8_87748346_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intron_Zfp423_chr8_87748346_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm3_intron_Dclk1_chr3_55437226_F | TCGTCGGCAGCGTCCACCAACTTTCACGTCACTTGA | 59.3 | 0.30 |
| mm3_intron_Dclk1_chr3_55437226_R | GTCTCGTGGGCTCGGACCCCAACTACAAAGCCAGG | 59.8 | 0.30 |
| mm4_intron_Rasgrp1_chr2_117301841_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_Rasgrp1_chr2_117301841_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Ptgfr|Gm25056_chr3_151843339_F | TCGTCGGCAGCGTCTCCAGTTGCCCAAGATCAGG | 59.6 | 0.28 |
| mm4_intergenic_Ptgfr|Gm25056_chr3_151843339_R | GTCTCGTGGGCTCGGTTTGTCTTGCTTAATTCATGTGGA | 57.3 | 0.28 |
| mm4_intron_Lrp6_chr6_134546199_F | TCGTCGGCAGCGTCGCATAGGCCAGTGCATACAAG | 59.3 | 0.27 |
| mm4_intron_Lrp6_chr6_134546199_R | GTCTCGTGGGCTCGGCTGATGTCCACCCTGGCTTC | 60.3 | 0.27 |
| mm4_exon_Pofut1_chr2_153261620_F | TCGTCGGCAGCGTCATCCCGAGGCCCTTTCTTTG | 60.0 | 0.27 |
| mm4_exon_Pofut1_chr2_153261620_R | GTCTCGTGGGCTCGGCAGAGGTCAAAGCTCCAGGG | 60.0 | 0.27 |
| mm4_intron_Vma21_chrX_71828961_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Vma21_chrX_71828961_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm3_intergenic_Gm26137|Gm23733_chr3_119968970_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm3_intergenic_Gm26137|Gm23733_chr3_119968970_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Diap3_chr14_86762554_F | TCGTCGGCAGCGTCTCTCGTCTACAAACAGCTCGT | 59.1 | 0.25 |
| mm4_intron_Diap3_chr14_86762554_R | GTCTCGTGGGCTCGGGGCTGGTAGGACTTGGACAG | 59.7 | 0.25 |
| mm4_intergenic_Tas1r2|Pax7_chr4_139699780_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Tas1r2|Pax7_chr4_139699780_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_5033403F01Rik|Gm23972_chr13_39301064_F | TCGTCGGCAGCGTCTCCTGAGGTAGGCTCCAGAA | 59.2 | 0.24 |
| mm4_intergenic_5033403F01Rik|Gm23972_chr13_39301064_R | GTCTCGTGGGCTCGGACATTGCAAACATTCATACCAGCT | 59.7 | 0.24 |
| mm4_intergenic_Gm23642|Lrfn2_chr17_48876652_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm23642|Lrfn2_chr17_48876652_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm7293|Arhgap20_chr9_51746353_F | TCGTCGGCAGCGTCTTAAGCCCCTTGACACCAGG | 59.5 | 0.21 |
| mm4_intergenic_Gm7293|Arhgap20_chr9_51746353_R | GTCTCGTGGGCTCGGCTTCCCAGTGTGCCCGTTAT | 60.0 | 0.21 |
| mm3_intron_Pax7_chr4_139797470_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm3_intron_Pax7_chr4_139797470_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm26713|RP23-125F8.1_chr9_81266348_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm26713|RP23-125F8.1_chr9_81266348_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Mbnl3_chrX_51171133_F | TCGTCGGCAGCGTCTGAAGCACAGGAAGCACACA | 60.1 | 0.18 |
| mm4_intron_Mbnl3_chrX_51171133_R | GTCTCGTGGGCTCGGTCACTCTGGGGTAGGAAGAACA | 60.1 | 0.18 |
| mm4_intron_Gfra2_chr14_70915343_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Gfra2_chr14_70915343_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intergenic_Syf2|Gm16225_chr4_135085130_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intergenic_Syf2|Gm16225_chr4_135085130_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm3272|Mmp2_chr8_92818450_F | TCGTCGGCAGCGTCTGAAACAGCTGGCCTGAGAG | 59.9 | 0.15 |
| mm4_intergenic_Gm3272|Mmp2_chr8_92818450_R | GTCTCGTGGGCTCGGAAATCGGATGCCCCTCACTG | 60.1 | 0.15 |
| mm4_intron_Dip2b_chr15_100202933_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Dip2b_chr15_100202933_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm5909|Gypa_chr8_80253962_F | TCGTCGGCAGCGTCGCCAGAAATAAAGACTGAGATCCG | 59.4 | 0.14 |
| mm4_intergenic_Gm5909|Gypa_chr8_80253962_R | GTCTCGTGGGCTCGGGTTCAGTTCCCCAGAGAGCC | 60.0 | 0.14 |
| mm4_intron_Frem1_chr4_82997822_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Frem1_chr4_82997822_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Setbp1_chr18_79018889_F | TCGTCGGCAGCGTCAGGTTGCTCTTTCATCTGCAGA | 59.9 | 0.14 |
| mm4_intron_Setbp1_chr18_79018889_R | GTCTCGTGGGCTCGGGCCGGTGTCAGGTACTTCAG | 60.3 | 0.14 |
| mm4_intron_Zwilch_chr9_64163169_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Zwilch_chr9_64163169_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_115846606_F | TCGTCGGCAGCGTCGTGGTGACTGGAAGAACCCC | 60.2 | 0.13 |
| mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_115846606_R | GTCTCGTGGGCTCGGTCTTGCATTTCTCTCTGCCTCT | 59.4 | 0.13 |
| mm4_exon_Nrp1_chr8_128434372_F | TCGTCGGCAGCGTCATGGGTGGACTCCAGGAGAA | 59.8 | 0.13 |
| mm4_exon_Nrp1_chr8_128434372_R | GTCTCGTGGGCTCGGAGCCTTTTCAGGTGCCTCAT | 59.5 | 0.13 |
| mm4_intron_Mfng_chr15_78767196_F | TCGTCGGCAGCGTCCGTTCATCTGGGCTGTCAGT | 60.0 | 0.12 |
| mm4_intron_Mfng_chr15_78767196_R | GTCTCGTGGGCTCGGTCTAGGGCATGGCTTTGGTG | 60.0 | 0.12 |
| mm4_intergenic_2410019O14Rik|Gm25407_chr2_157708239_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_2410019O14Rik|Gm25407_chr2_157708239_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Pten|Gm22482_chr19_32845073_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Pten|Gm22482_chr19_32845073_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Anxa4|Aak1_chr6_86794360_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Anxa4|Aak1_chr6_86794360_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Ubl4b|Alx3_chr3_107586896_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Ubl4b|Alx3_chr3_107586896_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Tmem19_chr10_115344772_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Tmem19_chr10_115344772_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Abcc12_chr8_86555831_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Abcc12_chr8_86555831_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Ncam2|Gm23355_chr16_81941833_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Ncam2|Gm23355_chr16_81941833_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Ptch1_chr13_63512771_F | TCGTCGGCAGCGTCGGACGGGGACACAATTCACT | 59.9 | 0.10 |
| mm4_intron_Ptch1_chr13_63512771_R | GTCTCGTGGGCTCGGGCTCCAAAACTCTGGCTTGC | 60.0 | 0.10 |
| mm3_intergenic_Slc9a6|Gm26312_chrX_56701210_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_Slc9a6|Gm26312_chrX_56701210_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Gcc2_chr10_58284295_F | TCGTCGGCAGCGTCAAGTCCTGTGCAGCAGCTAC | 60.3 | 0.10 |
| mm4_intron_Gcc2_chr10_58284295_R | GTCTCGTGGGCTCGGAGGTGTTGGCCTTTGTCTGA | 59.4 | 0.10 |
| mm4_intron_Gpr39_chr1_125693601_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Gpr39_chr1_125693601_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Pak6_chr2_118690469_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Pak6_chr2_118690469_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Fam172a_chr13_77831473_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Fam172a_chr13_77831473_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Rprml|Gosr2_chr11_103655753_F | TCGTCGGCAGCGTCTCGCAGCTCATCTATGGCTG | 59.9 | 0.09 |
| mm4_intergenic_Rprml|Gosr2_chr11_103655753_R | GTCTCGTGGGCTCGGCCCAGGCAGGAGGTGTTT | 59.1 | 0.09 |
| mm4_intron_Csmd1_chr8_16259644_F | TCGTCGGCAGCGTCGTTCACAAACATGGCCGCC | 60.3 | 0.08 |
| mm4_intron_Csmd1_chr8_16259644_R | GTCTCGTGGGCTCGGACTGCGTTGAACTTGGGTCA | 60.1 | 0.08 |
| mm4_intergenic_mmu-mir-378d|Gm24179_chr10_126769966_F | TCGTCGGCAGCGTCCTCATCATCTCCTGTGTGGCA | 59.7 | 0.08 |
| mm4_intergenic_mmu-mir-378d|Gm24179_chr10_126769966_R | GTCTCGTGGGCTCGGGTGCTGAGACTGTGGAGTGG | 60.3 | 0.08 |
| mm4_intron_Erp44_chr4_48223014_F | TCGTCGGCAGCGTCGGAGGAGAAGCAGGCAGAAA | 59.6 | 0.08 |
| mm4_intron_Erp44_chr4_48223014_R | GTCTCGTGGGCTCGGTGGCAGTGTGTGATCCTGAC | 59.9 | 0.08 |
| mm4_intergenic_Gm14132|Gm14131_chr2_149353040_F | TCGTCGGCAGCGTCCACACACTGAAGCCTGCATG | 59.7 | 0.07 |
| mm4_intergenic_Gm14132|Gm14131_chr2_149353040_R | GTCTCGTGGGCTCGGCCTGGGTGGAAAAGAGGTCC | 59.9 | 0.07 |
| mm4_intergenic_Gm22745|Kcnj6_chr16_94878555_F | TCGTCGGCAGCGTCTTGAGAAGCAGGGCAGACAG | 59.9 | 0.07 |
| mm4_intergenic_Gm22745|Kcnj6_chr16_94878555_R | GTCTCGTGGGCTCGGGCACATCCTTGCAAACGTGT | 59.9 | 0.07 |
| mm4_intergenic_Pitx1|H2afy_chr13_55936158_F | TCGTCGGCAGCGTCCTGGAACACTGGCCTCACAA | 60.1 | 0.07 |
| mm4_intergenic_Pitx1|H2afy_chr13_55936158_R | GTCTCGTGGGCTCGGCCACCATCACCCCTGTTGTT | 60.1 | 0.07 |
| mm4_intron_Exoc6_chr19_37657611_F | TCGTCGGCAGCGTCGGTTGTCGAACATTTGCCTCG | 60.4 | 0.07 |
| mm4_intron_Exoc6_chr19_37657611_R | GTCTCGTGGGCTCGGGCCTCTCGAGCTTATGTGGG | 60.2 | 0.07 |
| mm4_intron_Ncam1_chr9_49715402_F | TCGTCGGCAGCGTCCAGCCCAGGAAGATGTGGAG | 60.1 | 0.07 |
| mm4_intron_Ncam1_chr9_49715402_R | GTCTCGTGGGCTCGGAGATGCAAGTTTGGTTTGGCC | 59.9 | 0.07 |
| mm4_intergenic_AC158354.1|Gm4804_chr12_15612316_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_AC158354.1|Gm4804_chr12_15612316_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Calcoco1|Hoxc13_chr15_102811223_F | TCGTCGGCAGCGTCGTTTTGCTGCCCGAGAACTG | 60.0 | 0.06 |
| mm4_intergenic_Calcoco1|Hoxc13_chr15_102811223_R | GTCTCGTGGGCTCGGGCTGAGGGAACACCACTTGA | 59.8 | 0.06 |
| mm4_intron_Cherp_chr8_72461716_F | TCGTCGGCAGCGTCGGAGTGGCTACTTGAACCCC | 60.0 | 0.05 |
| mm4_intron_Cherp_chr8_72461716_R | GTCTCGTGGGCTCGGTACCAGACTCGAGGCTTGGA | 59.9 | 0.05 |
| mm4_intron_Taf5l_chr8_124013740_F | TCGTCGGCAGCGTCCAGCTGAACAGTGTCCTCGT | 59.9 | 0.05 |
| mm4_intron_Taf5l_chr8_124013740_R | GTCTCGTGGGCTCGGGGGACAGAATGGAGCTGAGG | 59.8 | 0.05 |
| mm4_intergenic_Gm25266|Cradd_chr10_95008323_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm25266|Cradd_chr10_95008323_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm15530|Arhgap31_chr16_38694223_F | TCGTCGGCAGCGTCGCCCTCTGGTGAAGTAAGGC | 60.3 | 0.05 |
| mm4_intergenic_Gm15530|Arhgap31_chr16_38694223_R | GTCTCGTGGGCTCGGCCCCTTCCCTGTCTTTGTCTC | 59.9 | 0.05 |
| mm4_intergenic_Gm15218|Gm8317_chr14_45797335_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm15218|Gm8317_chr14_45797335_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Dnmt3a_chr12_3877694_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Dnmt3a_chr12_3877694_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_n-R5s176|Rbm19_chr5_120072006_F | TCGTCGGCAGCGTCATCTTCCTCATCCCTGCCCT | 60.0 | 0.04 |
| mm4_intergenic_n-R5s176|Rbm19_chr5_120072006_R | GTCTCGTGGGCTCGGGCTGTGTGCCAATCAAAGCA | 59.9 | 0.04 |
| mm3_exon_Rin3_chr12_102313017_F | TCGTCGGCAGCGTCCCAGATGCTGGCAAAGGAGA | 60.0 | 0.04 |
| mm3_exon_Rin3_chr12_102313017_R | GTCTCGTGGGCTCGGCCAGCTTCTCCAGGACACTG | 60.0 | 0.04 |
| mm4_intergenic_Ier5l|Gm14487_chr2_30525900_F | TCGTCGGCAGCGTCAGCTCTCCCTTCCTCCTGTT | 59.8 | 0.04 |
| mm4_intergenic_Ier5l|Gm14487_chr2_30525900_R | GTCTCGTGGGCTCGGTTTTCATCGAGCCAGGCCTT | 59.9 | 0.04 |
| mm4_intron_Pdzd7_chr19_45028557_F | TCGTCGGCAGCGTCCGGGAGGATCCTAGAATCCCA | 60.2 | 0.04 |
| mm4_intron_Pdzd7_chr19_45028557_R | GTCTCGTGGGCTCGGGGCCAGGGAAGGAATCTGAG | 59.8 | 0.04 |
| mm4_intergenic_Gal|Ppp6r3_chr19_3439052_F | TCGTCGGCAGCGTCCTGTTTGGAGGGAGCAGAGG | 60.0 | 0.04 |
| mm4_intergenic_Gal|Ppp6r3_chr19_3439052_R | GTCTCGTGGGCTCGGGTCTGGGTCCCCTCTCATCT | 60.0 | 0.04 |
| mm4_intergenic_Isg20l2|Crabp2_chr3_87946460_F | TCGTCGGCAGCGTCTTCCCAGCTGCTCATGTCTG | 60.0 | 0.03 |
| mm4_intergenic_Isg20l2|Crabp2_chr3_87946460_R | GTCTCGTGGGCTCGGGCACCCCAACTTTGCTGAAT | 59.3 | 0.03 |
| mm4_intergenic_Map7|mmu-mir-6413_chr10_20290907_F | TCGTCGGCAGCGTCATGTCAGCAGTTTCTCGGGG | 60.0 | 0.03 |
| mm4_intergenic_Map7|mmu-mir-6413_chr10_20290907_R | GTCTCGTGGGCTCGGGTGGACAAGAACCAAGGGCT | 60.1 | 0.03 |
| mm4_intron_Clic5_chr17_44189885_F | TCGTCGGCAGCGTCCAGAAGCAGAACCCGGGAAA | 60.2 | 0.03 |
| mm4_intron_Clic5_chr17_44189885_R | GTCTCGTGGGCTCGGATCAGGCAGTAGAGTTCGCC | 59.5 | 0.03 |
| mm4_intron_Sh3bgrl2_chr9_83558455_F | TCGTCGGCAGCGTCGCGCTGCTAGAGTCTTGTCT | 59.8 | 0.03 |
| mm4_intron_Sh3bgrl2_chr9_83558455_R | GTCTCGTGGGCTCGGATGCAGTCTGAGACCTGGGA | 60.2 | 0.03 |
| mm4_intron_Chchd6_chr6_89383664_F | TCGTCGGCAGCGTCACAGTGGTATGGCTAACCCC | 59.0 | 0.03 |
| mm4_intron_Chchd6_chr6_89383664_R | GTCTCGTGGGCTCGGAGTTGCATTCTCCCAGTGCA | 59.8 | 0.03 |
| mm4_intergenic_Myom2|Gm24231_chr8_15160078_F | TCGTCGGCAGCGTCACGGTCACTAGACACAGGGA | 59.8 | 0.03 |
| mm4_intergenic_Myom2|Gm24231_chr8_15160078_R | GTCTCGTGGGCTCGGGGAAACCTTATCACGGGGCA | 60.0 | 0.03 |
| mm4_exon_Gm9990_chr7_97722496_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Gm9990_chr7_97722496_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Tac2|Zbtb39_chr10_127734334_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Tac2|Zbtb39_chr10_127734334_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Afg3l1_chr8_123502021_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Afg3l1_chr8_123502021_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Smarca2_chr19_26756019_F | TCGTCGGCAGCGTCAGTCAGAGAATGAATCCAGTCTTGT | 59.7 | 0.02 |
| mm4_intron_Smarca2_chr19_26756019_R | GTCTCGTGGGCTCGGCGGGGAGGGTCTAAGTGTCA | 60.6 | 0.02 |
| mm4_intergenic_Tiam1|Sod1_chr16_90066967_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tiam1|Sod1_chr16_90066967_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm13055|Gm13056_chr4_141272214_F | TCGTCGGCAGCGTCCCCCTGAGCTGAGAATGGTC | 59.8 | 0.02 |
| mm4_intergenic_Gm13055|Gm13056_chr4_141272214_R | GTCTCGTGGGCTCGGGATGTGACAGCAGCCGTTTC | 59.8 | 0.02 |
| mm4_intron_Atp2b2_chr6_113924066_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Atp2b2_chr6_113924066_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Znfx1_chr2_167040344_F | TCGTCGGCAGCGTCAGCTGGGGAGAGAAACAAGC | 59.9 | 0.02 |
| mm4_intron_Znfx1_chr2_167040344_R | GTCTCGTGGGCTCGGTTAACCCTGAACCTGCCAGC | 60.2 | 0.02 |
| mm4_intron_Gabbr2_chr4_46665893_F | TCGTCGGCAGCGTCCTCAGAGTCCCCAGAGCTCT | 60.0 | 0.02 |
| mm4_intron_Gabbr2_chr4_46665893_R | GTCTCGTGGGCTCGGGTGGCCAGGAGAAGAGAACC | 60.0 | 0.02 |
| mm4_intergenic_Fmnl1|Fmnl1/Gm20511_chr11_103192195_F | TCGTCGGCAGCGTCGCCTATCACACAGTAGGCCC | 59.8 | 0.02 |
| mm4_intergenic_Fmnl1|Fmnl1/Gm20511_chr11_103192195_R | GTCTCGTGGGCTCGGCACACCCAGAACTCTGCCTT | 59.8 | 0.02 |
| mm4_intergenic_Irf8|Gm26878_chr8_120848217_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Irf8|Gm26878_chr8_120848217_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Coq3|Faxc_chr4_21931108_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Coq3|Faxc_chr4_21931108_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Pycr2|Lefty1_chr1_180908617_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Pycr2|Lefty1_chr1_180908617_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_1700023L04Rik|Nrf1_chr6_30026997_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_1700023L04Rik|Nrf1_chr6_30026997_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm12708|Gm830_chr4_95209965_F | TCGTCGGCAGCGTCTTCTATGGGCCTCACCAGGA | 59.9 | 0.01 |
| mm4_intergenic_Gm12708|Gm830_chr4_95209965_R | GTCTCGTGGGCTCGGCACTGAAAACAAGGCTGGGC | 59.9 | 0.01 |
| mm4_intergenic_Gm13481|Gm13489_chr2_48668602_F | TCGTCGGCAGCGTCTGCACACTTTGAAGACCAGGA | 59.7 | 0.01 |
| mm4_intergenic_Gm13481|Gm13489_chr2_48668602_R | GTCTCGTGGGCTCGGTGCTAGAGATTTTGCTGTGCC | 58.9 | 0.01 |
| mm4_intergenic_1700057H15Rik|RP23-230F2.8_chr4_124502375_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_1700057H15Rik|RP23-230F2.8_chr4_124502375_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Spock2|Chst3_chr10_60179113_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Spock2|Chst3_chr10_60179113_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm11620|Tlk2_chr11_105178460_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm11620|Tlk2_chr11_105178460_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Gpc6_chr14_117537898_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Gpc6_chr14_117537898_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Usp22_chr11_61175035_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Usp22_chr11_61175035_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Nkx1-1|Fam53a_chr5_33557080_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Nkx1-1|Fam53a_chr5_33557080_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Helt|Acsl1_chr8_46332054_F | TCGTCGGCAGCGTCCAGGGCTCCATCACTAGCTG | 59.8 | 0.00 |
| mm4_intergenic_Helt|Acsl1_chr8_46332054_R | GTCTCGTGGGCTCGGCCCCTTTAGCAGTGTTCCTGT | 59.9 | 0.00 |
| mm4_intron_Pou6f2_chr13_18182803_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pou6f2_chr13_18182803_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Galnt12_chr4_47106815_F | TCGTCGGCAGCGTCGAGAGTGTTGAGCTGCCACT | 59.9 | 0.00 |
| mm4_intron_Galnt12_chr4_47106815_R | GTCTCGTGGGCTCGGAGAGAAGGCAAGTGGAGCTG | 59.6 | 0.00 |
| mm4_intron_Shroom3_chr5_92858872_F | TCGTCGGCAGCGTCGCCGGTCATTTTGAGAGCCT | 60.6 | 0.00 |
| mm4_intron_Shroom3_chr5_92858872_R | GTCTCGTGGGCTCGGCTGAAGGCACAGAGGGCTC | 60.0 | 0.00 |
| mm4_intron_Mvk_chr5_114458396_F | TCGTCGGCAGCGTCTTGTGACCTTGCTGGAGTGG | 60.1 | 0.00 |
| mm4_intron_Mvk_chr5_114458396_R | GTCTCGTGGGCTCGGGCTAAAGGCCAGTGAGGAGG | 60.1 | 0.00 |
| mm4_intron_Gm12153_chr11_44110184_F | TCGTCGGCAGCGTCGTTAGCCGGCTGACAGAACT | 60.0 | 0.00 |
| mm4_intron_Gm12153_chr11_44110184_R | GTCTCGTGGGCTCGGTTTCCCTATGTGTGCGTGCT | 59.9 | 0.00 |
| mm4_intron_Dusp4_chr8_34814691_F | TCGTCGGCAGCGTCTTACCGAGTAGGAGCAGGGG | 60.3 | 0.00 |
| mm4_intron_Dusp4_chr8_34814691_R | GTCTCGTGGGCTCGGCCATCCCAGCCTTCTGAGTC | 59.8 | 0.00 |
| mm4_intron_Disc1_chr8_125148263_F | TCGTCGGCAGCGTCGCTTCCTAGCCATGGGTTACT | 59.5 | 0.00 |
| mm4_intron_Disc1_chr8_125148263_R | GTCTCGTGGGCTCGGAAGACACTGCCGAGGAAACC | 60.2 | 0.00 |
| mm4_intron_Ccdc13_chr9_121799854_F | TCGTCGGCAGCGTCCTCAGAGCCTGTGTGACTGG | 60.0 | 0.00 |
| mm4_intron_Ccdc13_chr9_121799854_R | GTCTCGTGGGCTCGGAAGCGTAAGGATGTGGTGGG | 60.0 | 0.00 |
| mm4_intron_Armc8_chr9_99568336_F | TCGTCGGCAGCGTCCTCCGTCCCGAGAAGCAAC | 60.4 | 0.00 |
| mm4_intron_Armc8_chr9_99568336_R | GTCTCGTGGGCTCGGGGTGCGGGTTCCCAGGAC | 62.7 | 0.00 |
| mm4_intron_4931429P17Rik_chr13_47975407_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_4931429P17Rik_chr13_47975407_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_n-R5s26|Tulp4_chr17_6155859_F | TCGTCGGCAGCGTCGCCAGGCTGTTTGTAGGGAA | 60.2 | 0.00 |
| mm4_intergenic_n-R5s26|Tulp4_chr17_6155859_R | GTCTCGTGGGCTCGGGATGAGCAGAGCTGGCAACT | 60.3 | 0.00 |
| mm4_intergenic_Wnt3a|Wnt9a_chr11_59305021_F | TCGTCGGCAGCGTCGTACAAGGGCTGACTCCAGG | 59.7 | 0.00 |
| mm4_intergenic_Wnt3a|Wnt9a_chr11_59305021_R | GTCTCGTGGGCTCGGGCGATGCATACTCGGTGAGA | 59.9 | 0.00 |
| mm4_intergenic_Tshz3|Zfp536_chr7_37001082_F | TCGTCGGCAGCGTCGGATGTGGGCAGCATCTTCT | 60.1 | 0.00 |
| mm4_intergenic_Tshz3|Zfp536_chr7_37001082_R | GTCTCGTGGGCTCGGGTTAATGGTCCATGGCCCCA | 60.0 | 0.00 |
| mm4_intergenic_Slc25a5|Gm14549/2310010G23Rik_chrX_36813509_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Slc25a5|Gm14549/2310010G23Rik_chrX_36813509_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pdzd2|Gm24135_chr15_12469148_F | TCGTCGGCAGCGTCTCACACACAAAGCGTCAGC | 58.9 | 0.00 |
| mm4_intergenic_Pdzd2|Gm24135_chr15_12469148_R | GTCTCGTGGGCTCGGTCCATTGCCAAACACTGGTC | 58.6 | 0.00 |
| mm4_intergenic_Mir3072/Mirg|Dio3os_chr12_109947025_F | TCGTCGGCAGCGTCATCTTTGGTCTCCCTCAGCC | 59.0 | 0.00 |
| mm4_intergenic_Mir3072/Mirg|Dio3os_chr12_109947025_R | GTCTCGTGGGCTCGGAGGGGCAGGACTAAGGGAAA | 60.1 | 0.00 |
| mm4_intergenic_Lgi1|Slc35g1_chr19_38311917_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Lgi1|Slc35g1_chr19_38311917_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm8888|Slc38a4_chr15_96873805_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm8888|Slc38a4_chr15_96873805_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm26264|Gm22530_chr6_81768318_F | TCGTCGGCAGCGTCTGTGTGCACGCTCACGAG | 60.6 | 0.00 |
| mm4_intergenic_Gm26264|Gm22530_chr6_81768318_R | GTCTCGTGGGCTCGGACCTGGTTAGTGCTCTGACT | 57.9 | 0.00 |
| mm4_intergenic_Capza3|Gm24174_chr6_140162988_F | TCGTCGGCAGCGTCCTTCCCTGAGGAGTGAAGCC | 59.7 | 0.00 |
| mm4_intergenic_Capza3|Gm24174_chr6_140162988_R | GTCTCGTGGGCTCGGCCATTGAGAGAGGCAGGGAC | 59.8 | 0.00 |
| mm4_intergenic_AI481877|Rpsa-ps11_chr4_59161372_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_AI481877|Rpsa-ps11_chr4_59161372_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_5031414D18Rik|Lrrc63_chr14_75075892_F | TCGTCGGCAGCGTCTGGAGGAAAACGCACACATCT | 60.2 | 0.00 |
| mm4_intergenic_5031414D18Rik|Lrrc63_chr14_75075892_R | GTCTCGTGGGCTCGGAGGGATGAGAAGGACTGGCT | 59.9 | 0.00 |
| mm4_intergenic_4930562A09Rik|Hspb8_chr5_116404323_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930562A09Rik|Hspb8_chr5_116404323_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Pkd1l2_chr8_117044526_F | TCGTCGGCAGCGTCACGGATGCATTGCTGGGTTA | 60.0 | 0.00 |
| mm4_exon_Pkd1l2_chr8_117044526_R | GTCTCGTGGGCTCGGGGAGTTGGCAAGGCTCTTCA | 60.2 | 0.00 |
| mm4_exon_Nacad_chr11_6599449_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Nacad_chr11_6599449_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Lnx1_chr5_74677602_F | TCGTCGGCAGCGTCGCAGCAGCGCCTTCATTTT | 60.0 | 0.00 |
| mm4_exon_Lnx1_chr5_74677602_R | GTCTCGTGGGCTCGGGCTCCAAGTCGGGTGCATTA | 60.3 | 0.00 |
| mm4_exon_Hmgn2_chr4_133967457_F | TCGTCGGCAGCGTCACGCAGTTTTGGCGGCAG | 61.9 | 0.00 |
| mm4_exon_Hmgn2_chr4_133967457_R | GTCTCGTGGGCTCGGCTCCCCTCCTCCCTTCCTC | 60.0 | 0.00 |
| mm3_intergenic_Gm6327|Ercc4_chr16_12764309_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Gm6327|Ercc4_chr16_12764309_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm3_intron_Dclk1_chr3_55437226 |
CACCAACTTTCACGTCACTTGATCCTTTTTAATCGATGTAACAGACTTGTTATCCCTCGCCAATCCTAGTGCTGCCTTCT TCCCAGCAATCCAAATGTTTATTCCTGGCTTTGTAGTTGGGGT |
| mm4_intergenic_Ptgfr|Gm25056_chr3_151843339 |
TCCAGTTGCCCAAGATCAGGGCTCTACTTCTTTAAGTTGATCACCCCCAGCCTAGTCCCTCTTTCTTCCCACTTCATATT ATACCACATGGTGTTGATTAATTAATCCACATGAATTAAGCAAGACAAA |
| mm4_intron_Lrp6_chr6_134546199 | GCATAGGCCAGTGCATACAAGTGTAGGATGGCTGCACTAGCCTAGGCATGCACCTGTGGAAGCCAGGGTGGACATCAG |
| mm4_exon_Pofut1_chr2_153261620 |
ATCCCGAGGCCCTTTCTTTGCTTGGAGCACTTTCTAGACCACCCGAGGCTAGTGTAGCCCTCCTCTTCTGTTCTCTGGGG TTTACCCTGGAGCTTTGACCTCTG |
| mm4_intron_Diap3_chr14_86762554 | TCTCGTCTACAAACAGCTCGTTCCCAGCCTAGTGCAGCATCCTTCCTTTGTACTGTCCAAGTCCTACCAGCC |
| mm4_intergenic_5033403F01Rik|Gm23972_chr13_39301064 |
TCCTGAGGTAGGCTCCAGAAAACAATCTCAAAGGAAGGAAGGATAACAAGCAGGTAGACAGCACTAGGCTTGGTGTTATT TTACCATATTTTCAGTAAATAAGAGATATATAAGCTGGTATGAATGTTTGCAATGT |
| mm4_intergenic_Gm7293|Arhgap20_chr9_51746353 |
TTAAGCCCCTTGACACCAGGAGATTATGTACTGGAAGGAGGCACTAGCCTTGGAAGCATATGGGACCCTGAGATAACGGG CACACTGGGAAG |
| mm4_intron_Mbnl3_chrX_51171133 |
TGAAGCACAGGAAGCACACATTGAACACATTATTAAGTTCTCTATCTCGCTGTCTTCATCCAAGCTTAGGGCTGTCTTCC TCCCAAGGCTCCGAAAATAGGAAATTGATATGTTCTTCCTACCCCAGAGTGA |
| mm4_intergenic_Gm3272|Mmp2_chr8_92818450 |
TGAAACAGCTGGCCTGAGAGAACCAACCAGAGCTCTGAAGGATGATGGCCTTCCCAGCCTGGTGCTTCCTCCCTCAGAGG CCTTGTGCCAGGAACAGTGAGGGGCATCCGATTT |
| mm4_intergenic_Gm5909|Gypa_chr8_80253962 |
GCCAGAAATAAAGACTGAGATCCGAACTTGGGAAGCCGGCTCTAGGCTGGGATCCAATTGTCTGAAGCGCTGTGAATTTT ATGAAGGTTCCAGCTATTAGGCTCTCTGGGGAACTGAAC |
| mm4_intron_Setbp1_chr18_79018889 |
AGGTTGCTCTTTCATCTGCAGACTGAAATCCAAGGAAGGCAGCACTATTCTTGGCTGTCTAACTGAAAGGCTGGAGACTA CTGAAGTACCTGACACCGGC |
| mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_115846606 |
GTGGTGACTGGAAGAACCCCCCACCCCCCGATCAATACCACACTTCCAGGTTTCATTAAGGCTTTTAATAGCTCCATGCT CCTCTGGAAGGCAGCACCAGGCTTGGATCCACTTAGTAGAGGCAGAGAGAAATGCAAGA |
| mm4_exon_Nrp1_chr8_128434372 |
ATGGGTGGACTCCAGGAGAAGACTCCTACAAGGAGTGGATCCAGGTAGGTGGCACAAGGCTGGGCACAGTTGAGACATAT GAGGCACCTGAAAAGGCT |
| mm4_intron_Mfng_chr15_78767196 |
CGTTCATCTGGGCTGTCAGTGAAGATGAATGTCTGTGGGAAGAAGGAGGCATCAGGCTAGGTTAATGGAGAGAGGCCCCC AATCCACAGCCCACCAAAGCCATGCCCTAGA |
| mm4_intron_Ptch1_chr13_63512771 |
GGACGGGGACACAATTCACTGGCCAGCCCTTCTCTCCTTCAAGGAAGGCTGCTCTAGCCTGGGACTGGAATACACATTTC CTGTAAACATGGTGGGGGCCTCAGGCAAGCCAGAGTTTTGGAGC |
| mm4_intron_Gcc2_chr10_58284295 |
AAGTCCTGTGCAGCAGCTACCAAGCAGTGGCTTCCGACACATAAGTGAAGAGGAGGAGGAAGGAGACACTAAGGTCGGCC ACACAGTAAAATCAGACAAAGGCCAACACCT |
| mm4_intergenic_Rprml|Gosr2_chr11_103655753 |
TCGCAGCTCATCTATGGCTGTCCAAGTGCGGCTCGGGGTGCTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNGAGGAGAGAGGAAGGAAGCACCAGGATGGGAAACACCTCCTGCCTGGG |
| mm4_intron_Csmd1_chr8_16259644 |
GTTCACAAACATGGCCGCCCACCCATGTGTGTCCCACACAGTCACCTAGTCTTTTGCCGACTTCCTCGCCATTTTGATCT CTCCAGACAAATCAAGGAAACAGCAGGAGATCTTACCTCCTGACCCAAGTTCAACGCAGT |
| mm4_intergenic_mmu-mir-378d|Gm24179_chr10_126769966 |
CTCATCATCTCCTGTGTGGCATTCTCTGCAGAAGAATGACACATTCCCTATAAACACAGCCTGGATCCCTAGACCAGTGC TGCCATCCTCCCCCAAGCCCTCCCGCCCCACTCCACAGTCTCAGCAC |
| mm4_intron_Erp44_chr4_48223014 |
GGAGGAGAAGCAGGCAGAAAACAGAGGAGGGCTGCTCTAGCCTGGGGTGCTTAGGGGACAATGTTTACTCAGGCAAGGAA CCTGTAACCCTGAGTCAGGATCACACACTGCCA |
| mm4_intergenic_Gm14132|Gm14131_chr2_149353040 |
CACACACTGAAGCCTGCATGCCACCCAGGAGGTCCTCAGTAATCAAACACAGATCCAAGGTGATATAGAACCCACCCACC CTGCCTAGGTCTGCCTTCCTCTGGACCTCTTTTCCACCCAGG |
| mm4_intergenic_Gm22745|Kcnj6_chr16_94878555 |
TTGAGAAGCAGGGCAGACAGGGGAGAGGACAGGCTAGGAAGGAGGCACTGGCCTGGGCCTCAGAAGACACGTTTGCAAGG ATGTGC |
| mm4_intergenic_Pitx1|H2afy_chr13_55936158 |
CTGGAACACTGGCCTCACAAACCAAGCCTTGGGCCTCCATCCTCACTTGACTGGTCTGACAGAAGTCCTTTTCCCAGCAG GCCTTGCTTTACTTACTTGAACAACAGGGGTGATGGTGG |
| mm4_intron_Exoc6_chr19_37657611 |
GGTTGTCGAACATTTGCCTCGGAGGGATGAGGCCCTAGGCTGGGTACCAGCACTGGGAAAGGGAAATACTTCCAACCCAC ATAAGCTCGAGAGGC |
| mm4_intron_Ncam1_chr9_49715402 |
CAGCCCAGGAAGATGTGGAGTCAGAGGGAGGCTGCCCAAGGCTGGGGTTCTTCCTGGCCAGGTAAAACATGCTTTCCTCC TTGTCCTAGGATAATGGTCATGGCCAAACCAAACTTGCATCT |
| mm4_intergenic_Calcoco1|Hoxc13_chr15_102811223 |
GTTTTGCTGCCCGAGAACTGCTCTCTCTACAGTGTTGTTCCTATGTCTTGCCTATCCTAGAACCACCTTCCTCAGGCCCA GGCTTGAATCAAGTGGTGTTCCCTCAGC |
| mm4_intron_Cherp_chr8_72461716 |
GGAGTGGCTACTTGAACCCCCAGCAGGATGCCAACATGTGGTCATGCAGGAAGGGGGCACTGGTCTGGGTATACTCACCC ATCTTCACCAGCATTTGATGGCCTTTATTCTCTTCTCCAAGCCTCGAGTCTGGTA |
| mm4_intron_Taf5l_chr8_124013740 |
CAGCTGAACAGTGTCCTCGTCTCAGCAGCGGATCCCTGCTTCAGGATCCGTTCTCCACCCTCCTTAGTGCAGCCTTCCTC CATTTCCTTCATCACCTCAGCTCCATTCTGTCCC |
| mm4_intergenic_Gm15530|Arhgap31_chr16_38694223 |
GCCCTCTGGTGAAGTAAGGCTTAATTACTTTGAATTTTTATAGTTTTAGAGGAGGAGGTGGGCACTGGGCTGGGGGGGGG GGGGGCTAATGCCAAGGAACCTTGAAGTCTCCCAAGAGACAAAGACAGGGAAGGGG |
| mm4_intergenic_n-R5s176|Rbm19_chr5_120072006 |
ATCTTCCTCATCCCTGCCCTAACTTTTCAATGCAGATGGCAACAACCTGGTCACCCTTCCAAGTGATATGAGAGGAAATG ATGGTGTCCGCCTACCCTTGTGCCACCTTCCTGAGATGCTTTGATTGGCACACAGC |
| mm3_exon_Rin3_chr12_102313017 |
CCAGATGCTGGCAAAGGAGAGGGAGAGGAAGATGAGGAGAAAGACGGCACGAGGCTTGGTCTGTCAACCACCCCGAGGAA CTGCATCCCACGCCGGGGCATCAGTGTCCTGGAGAAGCTGG |
| mm4_intergenic_Ier5l|Gm14487_chr2_30525900 |
AGCTCTCCCTTCCTCCTGTTCCTGAATGACTTGGTTTCTCTCCCAGCCCAGTGCTGCCTGCATCAATGTCTGCAGTAGAC ATGGTGACAACCAGGGACCTTCAGATAAGGGAGAAGGCCTGGCTCGATGAAAA |
| mm4_intron_Pdzd7_chr19_45028557 |
CGGGAGGATCCTAGAATCCCAGCCCCAAGTCCAGTCCCGCCTTCTTCCCATCTCTGGCTTCTGTCTCAAGTCTCAACCTC CAAACAGATTCTCCAACGATATACCTTAGTACTCAGATTCCTTCCCTGGCC |
| mm4_intergenic_Gal|Ppp6r3_chr19_3439052 |
CTGTTTGGAGGGAGCAGAGGGGCTGGGTGCCCTCCAAGCCTGGAGCTGCCTTGCTCTGCTTTGGGGGAAATGCATCTAAG ATCAGGTTGAAGGGCACAGATGAGAGGGGACCCAGAC |
| mm4_intergenic_Isg20l2|Crabp2_chr3_87946460 |
TTCCCAGCTGCTCATGTCTGCGATTCCCCTGGAATAAAAGGGCTGCTGGAGGCCAAAAGCCCTAGCCCTAGCATATTGCC CCCTCCCTCCCTCCCTCATTCAGCAAAGTTGGGGTGC |
| mm4_intergenic_Map7|mmu-mir-6413_chr10_20290907 |
ATGTCAGCAGTTTCTCGGGGAGAGGAAGGCAGGATTAGGGTCGGACCTCCCTTCCTGTATTATGGGCAGTTGTTCACCTT CTCATAGACAAAGCCCTTGGTTCTTGTCCAC |
| mm4_intron_Clic5_chr17_44189885 |
CAGAAGCAGAACCCGGGAAACCTGAGGCGGAGGAAAGCAGCCCTGGGCTGGGCTGCAGAGTCGCCAGGACCTTGGGGGAG GGGCGAACTCTACTGCCTGAT |
| mm4_intron_Sh3bgrl2_chr9_83558455 |
GCGCTGCTAGAGTCTTGTCTGTGACGAGCAGGATGGATGATTGCTGGTGAGACCTGCAGGCATAGAGGAAGGAGGATCTG GGCTGGGTCCCAGCTCTTATTCCCAGGTCTCAGACTGCAT |
| mm4_intron_Chchd6_chr6_89383664 |
ACAGTGGTATGGCTAACCCCCACCCTGGCCTAGTGCAGCCTTGCCCACTTCTGCTCTACCTAAGTAAGGTCCTCCCTGTT CTCTAGCTTAACAAGTAGGGATTTTCCAAGAGATGCACTGGGAGAATGCAACT |
| mm4_intergenic_Myom2|Gm24231_chr8_15160078 |
ACGGTCACTAGACACAGGGACAGCACCCAATGAGATTAATAATTACATGAATGTAAACTCCTTATTTTCTGCCTACAGGG ACAGGAGTGGTCCCAACCCTAGTGCCCCCTGCCCCTGCCCCGTGATAAGGTTTCC |
| mm4_intron_Smarca2_chr19_26756019 |
AGTCAGAGAATGAATCCAGTCTTGTTTCAAGAGATGTCCTTTGAAAATAGTTCTAAATGGGCATCCCCAGCCCAGGGCCA CCTTCTTCAGGTAGAAACATCCCCCTTTGTTCTCTGAGTGACACTTAGACCCTCCCCG |
| mm4_intergenic_Gm13055|Gm13056_chr4_141272214 |
CCCCTGAGCTGAGAATGGTCATTGGCAGGGCCTGGTGGCCCAGTGCTGATATGAGGGGCAAGGCCAGGGATCTTGAGAGG CAGGGGGCTCCAGGCTGGGTTATGAAACGGCTGCTGTCACATC |
| mm4_intron_Znfx1_chr2_167040344 |
AGCTGGGGAGAGAAACAAGCCTGAGAATTCTCAGTGGGAACACACGACAGCCCAGCAGCGTGGGCTTCAAGTGCCTGCTG GAAGGCGGCACTAGCCCAGGTGCTGGCAGGTTCAGGGTTAA |
| mm4_intron_Gabbr2_chr4_46665893 |
CTCAGAGTCCCCAGAGCTCTTGGGAAGAAGCCAGAGGAAGCCGGCACCGGACTGGGAGTGGGAGGAGGTACCTGCATGGC TATGGCGGAGGGCTAATAAGGGAAAAGGGGGCTGTCCTCCGGTTCTCTTCTCCTGGCCAC |
| mm4_intergenic_Fmnl1|Fmnl1/Gm20511_chr11_103192195 |
GCCTATCACACAGTAGGCCCACAGGAAGGCTGCTCTAGGGTGGGCTCCTCTTGTGTGTGTAGCAAGTACTGAATGGAAGG CAGAGTTCTGGGTGTG |
| mm4_intergenic_Gm12708|Gm830_chr4_95209965 |
TTCTATGGGCCTCACCAGGATAGCTGAAAGCTTTCTTCTTTCCTGAGGAAGGCTGCATTGGGGTGGGGGCCCAGCCTTGT TTTCAGTG |
| mm4_intergenic_Gm13481|Gm13489_chr2_48668602 |
TGCACACTTTGAAGACCAGGAATTAGAGTCACTTTTAGAAACTGTTTGATCTATATATAGGGCAAAGAACTATATAGAGG AAGGAAGCACTGGGCCGGGCCTATATGAGGCACAGCAAAATCTCTAGCA |
| mm4_intergenic_Helt|Acsl1_chr8_46332054 |
CAGGGCTCCATCACTAGCTGGATGCAGAGGCAACGAGAGGCAGGCGGCACTGAGCCTGGTACCTGAGGAAATATCTGTAT CCAAGACAGGAACACTGCTAAAGGGG |
| mm4_intron_Galnt12_chr4_47106815 |
GAGAGTGTTGAGCTGCCACTGACCTTCCTGTGGTGGCCACATAGAGAGCGAGGAAGGAGCCACGAGGCCTGGGATGCCAT TGGCGTGGCAGCTCCACTTGCCTTCTCT |
| mm4_intron_Shroom3_chr5_92858872 |
GCCGGTCATTTTGAGAGCCTAAGTAGGACAGGATCTCAACATATTTGAGATTCAGTTCTGTGTTTTTGATCAGCCAGAGG AAGGGTGGACTTGGCTGGGGAAGGAAAAATGAACCGAGCGAGCCCTCTGTGCCTTCAG |
| mm4_intron_Mvk_chr5_114458396 |
TTGTGACCTTGCTGGAGTGGCAGCCAGGTTCTTATTCCGAAAACCAGCCCGGGAGGGAGGCAGCAGTAGGGTTGGCACAC CGTATATGACACTTTCTCTCTCGCAAATAGTAAGCCCTCCTCACTGGCCTTTAGC |
| mm4_intron_Gm12153_chr11_44110184 |
GTTAGCCGGCTGACAGAACTGGTTGGTAATTGACTGCCTGCTCCATGCCAAATGCCTCCTTCCTCAAGATGAACTGGGAG CCCCTTTTCCTAGCACGCACACATAGGGAAA |
| mm4_intron_Dusp4_chr8_34814691 |
TTACCGAGTAGGAGCAGGGGGCTGTGACAGAGGTCAGAGGAGGGCGGAAGTAGGGTGGGCGCACAGTTGATGACTCAGAA GGCTGGGATGG |
| mm4_intron_Disc1_chr8_125148263 |
GCTTCCTAGCCATGGGTTACTTCCATGGTCATGTCAACCCAGCCAGGTGTCACCTNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNACTCCTCAGGTTATGGATGGTTTCCTCGGCAGTGTCTT |
| mm4_intron_Ccdc13_chr9_121799854 |
CTCAGAGCCTGTGTGACTGGGTCACCGACTAGGAACCTCTGAGCCATCTCCTACTGCACCCTGCTGCCCCAGCCTCCTGC CTCCTTCCTGAGCCTCTAAATGTGGCTGTGCTCCCACCACATCCTTACGCTT |
| mm4_intron_Armc8_chr9_99568336 |
CTCCGTCCCGAGAAGCAACAGCGCAGGGAGGAAGACGGGAGAAGGCTCGGCCAAACCCAAGCCGCGCCCATGGGCCGGCT GCCTGGAGCCCGACCTCCCCGCCGGCCGAACGGCCGCCGTCCTGGGAACCCGCACC |
| mm4_intergenic_n-R5s26|Tulp4_chr17_6155859 |
GCCAGGCTGTTTGTAGGGAATATGAGAAAGTCGGCAGTTGGCTTGGTGAGACTCCAGGAGAAGCATTCCCTTGGTGCGAG CCATGGGGTTTTCTTCTCAAATAGTTGCCAGCTCTGCTCATC |
| mm4_intergenic_Wnt3a|Wnt9a_chr11_59305021 |
GTACAAGGGCTGACTCCAGGCCTCTCTGTGCGTGGGCTGCCCAAGACTGCTGCCTCCTTCCTCTGCACTTGCTGGGTGGC CGAGGTGCCAGCTCCGCTCTCACCGAGTATGCATCGC |
| mm4_intergenic_Tshz3|Zfp536_chr7_37001082 |
GGATGTGGGCAGCATCTTCTAACAAGCACCTGTTAGAATGGAGGAGGAAGTTGGCAGAAGGCTGGGTGGCTCCAGGGCAT GCTTCCACCCCCTCCTTTAGGGAGTACTTCTATCTGGGGCCATGGACCATTAAC |
| mm4_intergenic_Pdzd2|Gm24135_chr15_12469148 |
TCACACACAAAGCGTCAGCTTTATGAAAATTCATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCGACCAGTGTTTGGCAATGGA |
| mm4_intergenic_Mir3072/Mirg|Dio3os_chr12_109947025 |
ATCTTTGGTCTCCCTCAGCCCTGCCCAGCCTTCTTCAGCCTTCCTCAGCCCCACTCATTTCCCTTTAGCTCTGCCCTCAT CCTTCAGTACTCCCTCTCCTCAGCCCCACCCACTTTCCCTTAGTCCTGCCCCT |
| mm4_intergenic_Gm26264|Gm22530_chr6_81768318 |
TGTGTGCACGCTCACGAGCGCACAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNTTGTCTAGATTTGAAGTCAGAGCACTAACCAGGT |
| mm4_intergenic_Capza3|Gm24174_chr6_140162988 |
CTTCCCTGAGGAGTGAAGCCGCTTTCAGTCCAAGCCTACTGCTCTCTTCCTCCTCCCTGCCAAGGTTCTGACTCCTAAGA TTCCTTCTTCTCTTCCACATTTTAAGTCCCTGCCTCTCTCAATGG |
| mm4_intergenic_5031414D18Rik|Lrrc63_chr14_75075892 |
TGGAGGAAAACGCACACATCTTTATCCCATCTTTCTCCCAGGCCACTGCCTCCTTCCTCCTTTCTTTGCCCCTGCAGGCC GGTAGCCAGTCCTTCTCATCCCT |
| mm4_exon_Pkd1l2_chr8_117044526 |
ACGGATGCATTGCTGGGTTACCCTTATGTCCACAGGCTCCTGGCTGTCCTCCATAGGGCAGAGGAAGGTGGCAGCAGGCA GGGTGAAGGTCACAGAGTCAGCGGCAGCAGTGTGCACTGAAGAGCCTTGCCAACTCC |
| mm4_exon_Lnx1_chr5_74677602 |
GCAGCAGCGCCTTCATTTTCCGAGCAGTCTGCCGCCTTCCTCCTGACCACCTCCTGCCAGCCCAGTGTAGCCTGTTTAAG CAAATCTATTCATTTCCCCACAATCAGAAGATAATAATGCACCCGACTTGGAGC |
| mm4_exon_Hmgn2_chr4_133967457 |
ACGCAGTTTTGGCGGCAGCGCGCTCCCCCGCCCGCCCAGCCGAGCGCCGCCTTCCCGCCCTTTCGGGTTTGAANNNNNNN NNNNNNNNNNNNNNNNNNNNNGGGAGGAAGGGAGGAGGGGAG |