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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| mm0_exon_Pop5_chr5_115238102_F | TCGTCGGCAGCGTCTGCGGTTCAAGCACAGGTAA | 60.1 | 1.00 |
| mm0_exon_Pop5_chr5_115238102_R | GTCTCGTGGGCTCGGCTCGCACAGAAGGTACCTGC | 60.7 | 1.00 |
| mm4_intron_Ssbp3_chr4_106915015_F | TCGTCGGCAGCGTCTGGAGACGGCACTTGTTCTG | 60.2 | 0.63 |
| mm4_intron_Ssbp3_chr4_106915015_R | GTCTCGTGGGCTCGGCTCAGACAGCACCCATCCAG | 60.1 | 0.63 |
| mm4_intergenic_Fam221a|Stk31_chr6_49395490_F | TCGTCGGCAGCGTCTGCGCTCGAGGCCTTGAG | 62.4 | 0.61 |
| mm4_intergenic_Fam221a|Stk31_chr6_49395490_R | GTCTCGTGGGCTCGGCTTACCGCCGAGCTTCCAC | 60.8 | 0.61 |
| mm4_intergenic_Rasgef1c|Rnf130_chr11_50025316_F | Primer3: not found at this Tm | N.d. | 0.48 |
| mm4_intergenic_Rasgef1c|Rnf130_chr11_50025316_R | Primer3: not found at this Tm | N.d. | 0.48 |
| mm3_exon_Tspan9_chr6_128143497_F | Primer3: not found at this Tm | N.d. | 0.47 |
| mm3_exon_Tspan9_chr6_128143497_R | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intergenic_Gm4895|Raet1e/H60b_chr10_22154503_F | TCGTCGGCAGCGTCGGAGGGGTGAGGAAAGGGA | 60.2 | 0.45 |
| mm4_intergenic_Gm4895|Raet1e/H60b_chr10_22154503_R | GTCTCGTGGGCTCGGCATTTTCCAGCCGCGCTC | 59.8 | 0.45 |
| mm4_exon_Ube2q2_chr9_55149524_F | TCGTCGGCAGCGTCCATTTTCCAGCCGCGCTC | 59.8 | 0.45 |
| mm4_exon_Ube2q2_chr9_55149524_R | GTCTCGTGGGCTCGGGGAGGGGTGAGGAAAGGGA | 60.2 | 0.45 |
| mm4_exon_Cnksr3_chr10_7211821_F | TCGTCGGCAGCGTCTCCGCTCAAGCCTGCATG | 60.4 | 0.42 |
| mm4_exon_Cnksr3_chr10_7211821_R | GTCTCGTGGGCTCGGCTGCATCTGGACAGCGGTAG | 60.5 | 0.42 |
| mm4_exon_Ulk2_chr11_61854922_F | TCGTCGGCAGCGTCGCGTACCCGGAGCTACCC | 61.9 | 0.35 |
| mm4_exon_Ulk2_chr11_61854922_R | GTCTCGTGGGCTCGGCAACAAGCGGAGGCAGCC | 61.7 | 0.35 |
| mm4_exon_Ttbk1_chr17_46447120_F | TCGTCGGCAGCGTCGAGGATGGAGGACAAGGTGC | 60.1 | 0.30 |
| mm4_exon_Ttbk1_chr17_46447120_R | GTCTCGTGGGCTCGGAGAAGTCACCAGTCACTGCC | 59.6 | 0.30 |
| mm4_intron_Mpdz_chr4_81442567_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Mpdz_chr4_81442567_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Hip1r_chr5_123989413_F | TCGTCGGCAGCGTCCTTGCGTCGTAGGGAGCTAG | 59.9 | 0.26 |
| mm4_intron_Hip1r_chr5_123989413_R | GTCTCGTGGGCTCGGAGTTCCCCATGTTCTCTGCG | 60.0 | 0.26 |
| mm4_exon_Jund/Gm11175_chr8_70699161_F | TCGTCGGCAGCGTCGTCGCTGGTGCTACTGGG | 60.1 | 0.26 |
| mm4_exon_Jund/Gm11175_chr8_70699161_R | GTCTCGTGGGCTCGGGTCAGCGCGTCTTTCTTCAG | 59.5 | 0.26 |
| mm3_exon_A730015C16Rik/Kti12_chr4_108848173_F | TCGTCGGCAGCGTCCTACGAGCTGTACTGCCTCG | 59.9 | 0.25 |
| mm3_exon_A730015C16Rik/Kti12_chr4_108848173_R | GTCTCGTGGGCTCGGCCGCACGCAGTAAAGTAAGC | 59.9 | 0.25 |
| mm4_exon_Tmem231_chr8_111933682_F | TCGTCGGCAGCGTCGATGTAGGTGAGCGCGGTG | 60.8 | 0.24 |
| mm4_exon_Tmem231_chr8_111933682_R | GTCTCGTGGGCTCGGTCATGGCGCTGTACCATCTC | 59.8 | 0.24 |
| mm4_intron_Lta4h_chr10_93453697_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Lta4h_chr10_93453697_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Dgkh|Vwa8_chr14_78849119_F | TCGTCGGCAGCGTCGGTAGCCGATCCTACCGG | 58.6 | 0.20 |
| mm4_intergenic_Dgkh|Vwa8_chr14_78849119_R | GTCTCGTGGGCTCGGGTCCGCCGGAAACTCCTTC | 60.7 | 0.20 |
| mm4_intron_Ncoa1_chr12_4476427_F | TCGTCGGCAGCGTCAGGTCACCGTGCTTGCTC | 59.9 | 0.19 |
| mm4_intron_Ncoa1_chr12_4476427_R | GTCTCGTGGGCTCGGAGTTAGTGGAGTGCGAGCAT | 59.1 | 0.19 |
| mm4_intron_Ncoa2_chr1_13221670_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Ncoa2_chr1_13221670_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_AU022754|Mirlet7c-2_chr15_85671365_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_AU022754|Mirlet7c-2_chr15_85671365_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intron_Tex261_chr6_83775617_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intron_Tex261_chr6_83775617_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intergenic_2410019O14Rik|Gm25407_chr2_157618646_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intergenic_2410019O14Rik|Gm25407_chr2_157618646_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Snx9|Synj2_chr17_5939867_F | TCGTCGGCAGCGTCTCTTCATCTCCACTGGACCAC | 59.0 | 0.17 |
| mm4_intergenic_Snx9|Synj2_chr17_5939867_R | GTCTCGTGGGCTCGGGTCACCCTCAACCACCTTCC | 60.2 | 0.17 |
| mm4_intron_Col14a1_chr15_55409527_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Col14a1_chr15_55409527_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_exon_Stk24_chr14_121378997_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Stk24_chr14_121378997_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intergenic_ATF7/Atf7|Gm25141_chr15_102625630_F | TCGTCGGCAGCGTCTGGCTTGAGTGGAAGGTTGG | 60.1 | 0.16 |
| mm3_intergenic_ATF7/Atf7|Gm25141_chr15_102625630_R | GTCTCGTGGGCTCGGCTTCACCTTCGGACCTCCAC | 60.0 | 0.16 |
| mm4_intergenic_1190002N15Rik|Gm24178_chr9_94538351_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_1190002N15Rik|Gm24178_chr9_94538351_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Tm9sf4_chr2_153195924_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Tm9sf4_chr2_153195924_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm13134|Prdm16_chr4_154636507_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm13134|Prdm16_chr4_154636507_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_intergenic_Chrdl2|Pold3_chr7_100042165_F | TCGTCGGCAGCGTCCAGCGCAGGGATCACTCTAG | 59.9 | 0.13 |
| mm3_intergenic_Chrdl2|Pold3_chr7_100042165_R | GTCTCGTGGGCTCGGAGTCACGAATCAAAGCCCGT | 59.9 | 0.13 |
| mm4_intron_Crmp1_chr5_37245650_F | TCGTCGGCAGCGTCCCGTGTCCAAAGGCTGTTTG | 59.9 | 0.12 |
| mm4_intron_Crmp1_chr5_37245650_R | GTCTCGTGGGCTCGGTCCCTTCCGATTCCCCTACT | 59.3 | 0.12 |
| mm4_intergenic_Gm5611|Chordc1_chr9_17289422_F | TCGTCGGCAGCGTCCAGCACCACAAAACCGTCAG | 59.9 | 0.10 |
| mm4_intergenic_Gm5611|Chordc1_chr9_17289422_R | GTCTCGTGGGCTCGGCCACTTCCTCAGGACTGGAC | 59.3 | 0.10 |
| mm4_intergenic_Fam78a|Ppapdc3_chr2_32088789_F | TCGTCGGCAGCGTCAGCCTCTGGGGAAAGACAGA | 60.1 | 0.10 |
| mm4_intergenic_Fam78a|Ppapdc3_chr2_32088789_R | GTCTCGTGGGCTCGGCCTGATCCTTCGCTCGGTTT | 60.1 | 0.10 |
| mm4_exon_Mafa_chr15_75747660_F | TCGTCGGCAGCGTCCTTTTCCTGAGGCGCCCC | 60.7 | 0.09 |
| mm4_exon_Mafa_chr15_75747660_R | GTCTCGTGGGCTCGGCCTCTTCGCCCAGCTTCTG | 60.4 | 0.09 |
| mm4_exon_Hras_chr7_141193989_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Hras_chr7_141193989_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Irs4/Gm15295|Gm15295_chrX_141726006_F | TCGTCGGCAGCGTCGGCTGTAAACAACCCTCGGA | 59.9 | 0.08 |
| mm4_intergenic_Irs4/Gm15295|Gm15295_chrX_141726006_R | GTCTCGTGGGCTCGGACTGCGATCGGAAGGGAAAA | 59.6 | 0.08 |
| mm4_intergenic_Dpy30|Spast_chr17_74338740_F | TCGTCGGCAGCGTCTCCCAGGTCAACAAAGACGG | 59.8 | 0.08 |
| mm4_intergenic_Dpy30|Spast_chr17_74338740_R | GTCTCGTGGGCTCGGCTGCCCCTCCTTTCCTTCTG | 60.0 | 0.08 |
| mm4_intron_Chst9_chr18_15759900_F | TCGTCGGCAGCGTCAGCCACAATGGTACCTTGCA | 59.8 | 0.07 |
| mm4_intron_Chst9_chr18_15759900_R | GTCTCGTGGGCTCGGGTAACTGCACTGGGGCTAGG | 60.1 | 0.07 |
| mm4_intron_Sdk2_chr11_113981971_F | TCGTCGGCAGCGTCGGTCCCCAGAGTTTGAAGGG | 59.9 | 0.07 |
| mm4_intron_Sdk2_chr11_113981971_R | GTCTCGTGGGCTCGGCTGCCTCTGTATCCACTCGC | 60.2 | 0.07 |
| mm4_intron_Zc3h18_chr8_122376841_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Zc3h18_chr8_122376841_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Rere|Slc45a1_chr4_150627778_F | TCGTCGGCAGCGTCCACCCTTTCCCCAGTTCTCC | 59.9 | 0.07 |
| mm4_intergenic_Rere|Slc45a1_chr4_150627778_R | GTCTCGTGGGCTCGGCTCTAGCCACCTTCTGCCAC | 60.1 | 0.07 |
| mm4_exon_Fam198a_chr9_121978443_F | TCGTCGGCAGCGTCCCTCACACTGTTCAGGGACA | 59.2 | 0.07 |
| mm4_exon_Fam198a_chr9_121978443_R | GTCTCGTGGGCTCGGTGCTGTCATTGAGAGGGACG | 59.7 | 0.07 |
| mm4_exon_Ahctf1/Gm10518_chr1_179803545_F | TCGTCGGCAGCGTCCTTCTCGCTCAGTCTCTCGC | 60.2 | 0.07 |
| mm4_exon_Ahctf1/Gm10518_chr1_179803545_R | GTCTCGTGGGCTCGGCCAGCCCAGTAAGTCGAGTC | 59.8 | 0.07 |
| mm4_intergenic_n-R5s96|Cldn24_chr8_47793063_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_n-R5s96|Cldn24_chr8_47793063_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Pitx1_chr13_55831402_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Pitx1_chr13_55831402_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm2_exon_Slc12a5_chr2_164997920_F | TCGTCGGCAGCGTCAGAAACGCAACGGACTCCTC | 60.3 | 0.05 |
| mm2_exon_Slc12a5_chr2_164997920_R | GTCTCGTGGGCTCGGAAAGGGAAAGGAGAGCGCC | 60.0 | 0.05 |
| mm4_exon_Cdh22_chr2_165181287_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Cdh22_chr2_165181287_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Rasgef1b_chr5_99252858_F | TCGTCGGCAGCGTCCGAACGCCAGAAGAAAGGGA | 60.3 | 0.05 |
| mm4_exon_Rasgef1b_chr5_99252858_R | GTCTCGTGGGCTCGGCAGGAGGCGGGCATTTCC | 60.8 | 0.05 |
| mm4_exon_Fbxw4_chr19_45582784_F | TCGTCGGCAGCGTCCTCAGAACAGAAGGCAGGGC | 60.6 | 0.05 |
| mm4_exon_Fbxw4_chr19_45582784_R | GTCTCGTGGGCTCGGTGGGCCACAGTGGTTAATGG | 60.2 | 0.05 |
| mm4_intergenic_Smim17|Gm20715_chr7_6438636_F | TCGTCGGCAGCGTCAAAGGTAGATGGGGCCCATG | 59.4 | 0.05 |
| mm4_intergenic_Smim17|Gm20715_chr7_6438636_R | GTCTCGTGGGCTCGGTGCTCTCTCCCTAGGTCAGG | 59.7 | 0.05 |
| mm4_intergenic_Gm9341|Gm24444_chr7_136020226_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm9341|Gm24444_chr7_136020226_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Ncoa1|Gm22343_chr12_4376002_F | TCGTCGGCAGCGTCGCAGCTGAGCAATGGAGAGT | 60.3 | 0.04 |
| mm4_intergenic_Ncoa1|Gm22343_chr12_4376002_R | GTCTCGTGGGCTCGGTTGCATGTGCTGACAGACCA | 60.1 | 0.04 |
| mm4_exon_Tenm3_chr8_48555132_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Tenm3_chr8_48555132_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Rell2_chr18_37956354_F | TCGTCGGCAGCGTCCAGCCCACTAAACCCAGGAG | 60.0 | 0.04 |
| mm4_exon_Rell2_chr18_37956354_R | GTCTCGTGGGCTCGGAGCACAAGCAGGAAGAGCAT | 59.9 | 0.04 |
| mm4_exon_Kcnh2_chr5_24351564_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Kcnh2_chr5_24351564_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Dyrk1a_chr16_94570455_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Dyrk1a_chr16_94570455_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Dgcr6_chr16_18066661_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Dgcr6_chr16_18066661_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Hivep1_chr13_42052251_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Hivep1_chr13_42052251_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Tll1_chr8_64205752_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Tll1_chr8_64205752_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Plcd1|Dlec1_chr9_119100668_F | TCGTCGGCAGCGTCGCTGCTAGTGACAGGATCCC | 59.8 | 0.03 |
| mm4_intergenic_Plcd1|Dlec1_chr9_119100668_R | GTCTCGTGGGCTCGGTGTGGGGAGAGTAGCAGAGT | 59.5 | 0.03 |
| mm4_intergenic_Plcd1|Dlec1_chr9_119100645_F | TCGTCGGCAGCGTCGCTGCTAGTGACAGGATCCC | 59.8 | 0.03 |
| mm4_intergenic_Plcd1|Dlec1_chr9_119100645_R | GTCTCGTGGGCTCGGGTAGCAGAGTAGCAGCCGAG | 59.9 | 0.03 |
| mm3_exon_Tmem41b_chr7_109986350_F | TCGTCGGCAGCGTCCCAGTAACCGCGAGACAAGT | 60.0 | 0.03 |
| mm3_exon_Tmem41b_chr7_109986350_R | GTCTCGTGGGCTCGGGCGGAGAAGTCGGACCTTAG | 59.8 | 0.03 |
| mm4_intron_Adamts15_chr9_30918904_F | TCGTCGGCAGCGTCACGACTCTGGCTCTGACTCT | 59.9 | 0.03 |
| mm4_intron_Adamts15_chr9_30918904_R | GTCTCGTGGGCTCGGGATTTTGCAAAGCAGCCAGC | 59.2 | 0.03 |
| mm4_exon_Ikzf1_chr11_11686065_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Ikzf1_chr11_11686065_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Cdk5rap3_chr11_96911434_F | TCGTCGGCAGCGTCCTGGTCTCAGGAAAGCCAGG | 60.0 | 0.03 |
| mm4_exon_Cdk5rap3_chr11_96911434_R | GTCTCGTGGGCTCGGGGAGCTGTTTCCCATCCACA | 59.9 | 0.03 |
| mm4_intergenic_Tbxas1|Hipk2_chr6_38875765_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tbxas1|Hipk2_chr6_38875765_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Myo5b_chr18_74442800_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Myo5b_chr18_74442800_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Gnas/Gm20721_chr2_174330427_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Gnas/Gm20721_chr2_174330427_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm9867|Gm13016_chr4_140355857_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm9867|Gm13016_chr4_140355857_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_mmu-mir-7031|Sh2b3_chr5_121820781_F | TCGTCGGCAGCGTCACAAATTCCATGCTCGTGGC | 59.4 | 0.02 |
| mm4_intergenic_mmu-mir-7031|Sh2b3_chr5_121820781_R | GTCTCGTGGGCTCGGGGGACAGAGCACTTCCCAAG | 60.3 | 0.02 |
| mm4_intergenic_Cep68|Rpl12-ps2_chr11_20249605_F | TCGTCGGCAGCGTCAGCTTCTTTCTCCGCTGTCC | 60.0 | 0.02 |
| mm4_intergenic_Cep68|Rpl12-ps2_chr11_20249605_R | GTCTCGTGGGCTCGGAAACACCGCAAGCCTTCGAT | 60.8 | 0.02 |
| mm4_intron_Ttc7b_chr12_100520444_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Ttc7b_chr12_100520444_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Iars2_chr1_185292760_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Iars2_chr1_185292760_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Rbm10_chrX_20645590_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Rbm10_chrX_20645590_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Acads_chr5_115119330_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Acads_chr5_115119330_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_B4galt5_chr2_167349060_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_B4galt5_chr2_167349060_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Gm8619_chr13_48871158_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Gm8619_chr13_48871158_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ano6_chr15_95919592_F | TCGTCGGCAGCGTCAGTGCACAGACCAAGACAGA | 58.8 | 0.01 |
| mm4_intron_Ano6_chr15_95919592_R | GTCTCGTGGGCTCGGCAGGAAAAGACTGTTCCCGC | 59.1 | 0.01 |
| mm4_intergenic_Redrum|Zfp608_chr18_54487201_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Redrum|Zfp608_chr18_54487201_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Pcna-ps1|Tmem28_chrX_99820900_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Pcna-ps1|Tmem28_chrX_99820900_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_E130102H24Rik|Gm12795_chr4_101356574_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_E130102H24Rik|Gm12795_chr4_101356574_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_3110035E14Rik_chr1_9613181_F | TCGTCGGCAGCGTCTGCAACAACCACCTTAGCCT | 59.8 | 0.01 |
| mm4_intron_3110035E14Rik_chr1_9613181_R | GTCTCGTGGGCTCGGGCACCCACCTAATGTCCACT | 59.6 | 0.01 |
| mm4_exon_Dip2a_chr10_76345261_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Dip2a_chr10_76345261_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Tmem132d_chr5_128015002_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Tmem132d_chr5_128015002_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Tuba3a_chr6_125285951_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Tuba3a_chr6_125285951_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Ebf1_chr11_44620410_F | TCGTCGGCAGCGTCCGGGACACAGTCTGGCTTAG | 60.1 | 0.01 |
| mm4_exon_Ebf1_chr11_44620410_R | GTCTCGTGGGCTCGGTTAGAAGGCGGCTGCTTCTC | 60.1 | 0.01 |
| mm3_exon_Gnai1_chr5_18360035_F | TCGTCGGCAGCGTCCTGTTGAGGGACTGGGATGC | 60.3 | 0.01 |
| mm3_exon_Gnai1_chr5_18360035_R | GTCTCGTGGGCTCGGCGGGAGGTCAAGCTGCTG | 60.4 | 0.01 |
| mm4_intron_Dusp27_chr1_166124045_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Dusp27_chr1_166124045_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Crocc_chr4_141021778_F | TCGTCGGCAGCGTCACACTCTCTTCCTGCAGTGC | 59.9 | 0.01 |
| mm4_exon_Crocc_chr4_141021778_R | GTCTCGTGGGCTCGGGGAGAGGGTGGGGTACAGAT | 60.0 | 0.01 |
| mm4_exon_Rufy1_chr11_50430819_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Rufy1_chr11_50430819_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm8325|P2ry1_chr3_61002742_F | TCGTCGGCAGCGTCCTTGCACGGCGGAGACAC | 61.4 | 0.01 |
| mm4_intergenic_Gm8325|P2ry1_chr3_61002742_R | GTCTCGTGGGCTCGGCAGGAGAGAAAGCCAGGCTC | 60.1 | 0.01 |
| mm4_intron_Camk2b_chr11_5988780_F | TCGTCGGCAGCGTCTCCTTTAGCCCTCAGGGTGA | 59.8 | 0.00 |
| mm4_intron_Camk2b_chr11_5988780_R | GTCTCGTGGGCTCGGGAGGAAGCAGGAGAAACCCC | 60.0 | 0.00 |
| mm4_exon_Akap8l_chr17_32332462_F | TCGTCGGCAGCGTCGGCTCAAATTCCAGGCAAGC | 60.1 | 0.00 |
| mm4_exon_Akap8l_chr17_32332462_R | GTCTCGTGGGCTCGGTGCAGCCTGTGACCTTTTCA | 60.1 | 0.00 |
| mm4_exon_Cry2_chr2_92413634_F | TCGTCGGCAGCGTCTCACAGGGTACAGGCTAGCT | 59.9 | 0.00 |
| mm4_exon_Cry2_chr2_92413634_R | GTCTCGTGGGCTCGGTCATGACCCAACTGAGGCAG | 59.6 | 0.00 |
| mm4_exon_Cacna1a_chr8_84639639_F | TCGTCGGCAGCGTCCAGGCTGCCCAATGGTTACT | 60.3 | 0.00 |
| mm4_exon_Cacna1a_chr8_84639639_R | GTCTCGTGGGCTCGGCTTAGCACCAGTCGTCCTCG | 60.1 | 0.00 |
| mm4_exon_Epha5_chr5_84417176_F | TCGTCGGCAGCGTCGCTGGCAGAGGGAAGTCAAA | 60.2 | 0.00 |
| mm4_exon_Epha5_chr5_84417176_R | GTCTCGTGGGCTCGGCTCTGCTGCGCTCTCGGG | 62.8 | 0.00 |
| mm3_exon_Hus1_chr11_9010980_F | TCGTCGGCAGCGTCGCAAAAGGAAGGTCCCTGGT | 60.1 | 0.00 |
| mm3_exon_Hus1_chr11_9010980_R | GTCTCGTGGGCTCGGCATTTCACACGTGAGCTGGC | 60.1 | 0.00 |
| mm4_exon_Lrrc59_chr11_94629796_F | TCGTCGGCAGCGTCACGGAAGTGGTCGTCGTC | 59.3 | 0.00 |
| mm4_exon_Lrrc59_chr11_94629796_R | GTCTCGTGGGCTCGGCCACGCCTGCGCCTTAAG | 61.5 | 0.00 |
| mm4_intergenic_Gm15684|Crispld2_chr8_120046608_F | TCGTCGGCAGCGTCGCTACTCAGTGGACAGAGCC | 59.8 | 0.00 |
| mm4_intergenic_Gm15684|Crispld2_chr8_120046608_R | GTCTCGTGGGCTCGGTGCCAAGACCAAGCAGAGAG | 59.9 | 0.00 |
| mm4_intergenic_Ppp1r2-ps2|Nup210_chr6_90988552_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ppp1r2-ps2|Nup210_chr6_90988552_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm5903|Ate1_chr7_130369514_F | TCGTCGGCAGCGTCTTGGGGAATCCTGGTTCTGC | 59.9 | 0.00 |
| mm4_intergenic_Gm5903|Ate1_chr7_130369514_R | GTCTCGTGGGCTCGGATGCTTTGCTCACCTTGGTG | 59.0 | 0.00 |
| mm4_intergenic_A230083G16Rik|Ptms_chr6_124917436_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_A230083G16Rik|Ptms_chr6_124917436_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm12718|Gm23064_chr4_103445091_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm12718|Gm23064_chr4_103445091_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Nccrp1_chr7_28543894_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Nccrp1_chr7_28543894_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Bmp2k_chr5_96997672_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Bmp2k_chr5_96997672_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Scyl3_chr1_163930236_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Scyl3_chr1_163930236_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_n-R5s86|Wdr72_chr9_74110264_F | TCGTCGGCAGCGTCTATAGGAGGGCGGTGGGAAG | 60.4 | 0.00 |
| mm4_intergenic_n-R5s86|Wdr72_chr9_74110264_R | GTCTCGTGGGCTCGGGAACCAAGCCCACAGGTGA | 59.8 | 0.00 |
| mm4_intergenic_Stx1b/Stx4a|Stx4a_chr7_127841125_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Stx1b/Stx4a|Stx4a_chr7_127841125_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Lsm4|Jund/Gm11175_chr8_70689415_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Lsm4|Jund/Gm11175_chr8_70689415_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ktn1|Gm6055_chr14_47840127_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ktn1|Gm6055_chr14_47840127_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm14513|Gm26314_chrX_11807691_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm14513|Gm26314_chrX_11807691_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Klc1_chr12_111778085_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Klc1_chr12_111778085_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Itfg1_chr8_85840690_F | TCGTCGGCAGCGTCCCTCAGCCCCAAAGAGCTC | 60.0 | 0.00 |
| mm4_exon_Itfg1_chr8_85840690_R | GTCTCGTGGGCTCGGGGCAGGCCGGCTTCCTAG | 62.5 | 0.00 |
| mm4_exon_Gpr88_chr3_116251658_F | TCGTCGGCAGCGTCGAACTCCTCGTTCCTCCACG | 60.1 | 0.00 |
| mm4_exon_Gpr88_chr3_116251658_R | GTCTCGTGGGCTCGGCTGGTGTGGGTGAGCCTG | 59.9 | 0.00 |
| mm4_exon_Fam43b/AB041806_chr4_138394177_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Fam43b/AB041806_chr4_138394177_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_D030028A08Rik/Sp2_chr11_96954360_F | TCGTCGGCAGCGTCGGGACACAGCCACAGAACAG | 60.8 | 0.00 |
| mm4_exon_D030028A08Rik/Sp2_chr11_96954360_R | GTCTCGTGGGCTCGGGGAGGCCCTGAAGATGGAAC | 60.1 | 0.00 |
| mm3_intron_Mfsd6_chr1_52726948_F | TCGTCGGCAGCGTCCTGCACCCCGAGATGACAG | 60.1 | 0.00 |
| mm3_intron_Mfsd6_chr1_52726948_R | GTCTCGTGGGCTCGGATCCTCCTCCGTGCACCT | 59.9 | 0.00 |
| mm3_intergenic_Gabpb1|Gm27003/Gabpb1_chr2_126675809_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Gabpb1|Gm27003/Gabpb1_chr2_126675809_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm2_intron_Ephb2_chr4_136835508_F | TCGTCGGCAGCGTCGCGCAGTGGTAAGACCCC | 60.4 | 0.00 |
| mm2_intron_Ephb2_chr4_136835508_R | GTCTCGTGGGCTCGGTGATTGACTGTGCCAGGAGG | 59.6 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm0_exon_Pop5_chr5_115238102 |
TGCGGTTCAAGCACAGGTAAGCAGCGCTGTCCCCGCGCCGTCCCCGCGACCCGGGCCGCGCTCACCCCGCTGTCGCCGCA GGTACCTTCTGTGCGAG |
| mm4_intron_Ssbp3_chr4_106915015 |
TGGAGACGGCACTTGTTCTGTTCTTGTGACTGTGGAGTGTTGGAGTGATCACAGAGAATTACAGAGGGTCTTGAGTGCTG TGCCCTGGGCTGCGCTCACCCTGCCCACTTTCTCTGTACTGGATGGGTGCTGTCTGAG |
| mm4_intergenic_Fam221a|Stk31_chr6_49395490 |
TGCGCTCGAGGCCTTGAGGCCGGCCACCCACGTGACCAGGGCTGCGCTCCCTCCGCTATAGCGCAGCAGCACCTCGCGCA TGCGCAGGCGCAGTGCGTGCCCTGAGGCGTGGAAGCTCGGCGGTAAG |
| mm4_intergenic_Gm4895|Raet1e/H60b_chr10_22154503 |
GGAGGGGTGAGGAAAGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN TCTCGCGGCTCCGCCTCTCCCCGCCGCCGCGACCCTCGTCCGAGCGCGGCTGGAAAATG |
| mm4_exon_Ube2q2_chr9_55149524 |
CATTTTCCAGCCGCGCTCGGACGCGGGTCGCGGCGGCGGGGAGAGGCGGAGCCGCGAGANNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCCTTTCCTCACCCCTCC |
| mm4_exon_Cnksr3_chr10_7211821 |
TCCGCTCAAGCCTGCATGGCCGCAGTCAGCGGGCCATCGCGCTGCGGTTTCTGCGAGGGAAGCGCGGCCCTGGGTCTCTG CGCACGGGACAGCTATGTCCCGTACGGCTGCTCCCCGCAGCTACCGCTGTCCAGATGCAG |
| mm4_exon_Ulk2_chr11_61854922 |
GCGTACCCGGAGCTACCCGCGCGCCTGACAGCGGTGCAGGGCCGGGGCCGCGCCATCCCCGCAGGTGCGGCTGCCTCCGC TTGTTG |
| mm4_exon_Ttbk1_chr17_46447120 |
GAGGATGGAGGACAAGGTGCCTGGCTCTGATACGTCCAGCTGGCTTCCAGTGGGCTGAGGCCGCTCGTGCTGAGGGGTGA GCGCAGCCAAGGTGCCCAGATCAGGGTCGGGGGCCAGTTCGGCAGTGACTGGTGACTTCT |
| mm4_intron_Hip1r_chr5_123989413 |
CTTGCGTCGTAGGGAGCTAGCTCTCAGGAGACTGTGGGCTGAGCACGGCCCTGGCCCAGGGTGTGGGAGTTGGGAGAGCA TGGCCCCAGGACTCGCAGAGAACATGGGGAACT |
| mm4_exon_Jund/Gm11175_chr8_70699161 |
GTCGCTGGTGCTACTGGGGCCCCCGGCGGTGGTGGCTTCGCGCCCCCGGGCCGCGCTTTCCCCGGGGCGCCCCCGACGAG CAGCATGCTGAAGAAAGACGCGCTGAC |
| mm3_exon_A730015C16Rik/Kti12_chr4_108848173 | CTACGAGCTGTACTGCCTCGCCCGGGCCGCGCGCACGCCGCTCTGCTTACTTTACTGCGTGCGG |
| mm4_exon_Tmem231_chr8_111933682 |
GATGTAGGTGAGCGCGGTGGTCAACAGCAGGAAGAGCGCGGCCTTGGAGCAGAGCCCCGCGCGGTAAGCGCGCTCGATTG GGTGAGAGAAGAGATGGTACAGCGCCATGA |
| mm4_intergenic_Dgkh|Vwa8_chr14_78849119 |
GGTAGCCGATCCTACCGGCTGGCGCTCGGCCTGCCCTCCGCCCCGGGCCGGGCCCATCCCGCAGAGCCCGGCGAGCCGGG GGAAGGAGTTTCCGGCGGAC |
| mm4_intron_Ncoa1_chr12_4476427 |
AGGTCACCGTGCTTGCTCGTCGGGGCCGAGGGGCGCCCGCGCCTACTTCCGGCTCCCCCTTTNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCGCGGATGCTCGCACTCCACTAACT |
| mm4_intergenic_Snx9|Synj2_chr17_5939867 |
TCTTCATCTCCACTGGACCACCCAGGGCTGCTGTCACCCCTCTGGATGGAGCCGCTGTTTATTTTGTTTGGATACAGAAA AGGGAAGGTGGTTGAGGGTGAC |
| mm3_intergenic_ATF7/Atf7|Gm25141_chr15_102625630 |
TGGCTTGAGTGGAAGGTTGGGCGAGCGCTGTACACACGACTCGCACGTGTTCGGCTGCAAAGTGTGGGCAGCGCGGGGAG AGCGGCCCTGGCGTCTAACGCTGCCGATCTGTCTGTAGCTGTGGAGGTCCGAAGGTGAAG |
| mm3_intergenic_Chrdl2|Pold3_chr7_100042165 |
CAGCGCAGGGATCACTCTAGAGGCAAGACTCGAACTCCTCCACTGCCCGCTGCTGCCAGCAGGGTGCTCGCGGCCCGGGA CAGGGGGCGGGGGTCGGGGCACCGGCGCAGAAGAACACGGGCTTTGATTCGTGACT |
| mm4_intron_Crmp1_chr5_37245650 |
CCGTGTCCAAAGGCTGTTTGTTTCTTGCTTTTAAAACACCACTCAAAAGCATATTCTCCTAGTGGCGTGAGCGAGGCCCC GGCTTAAGCGAGGGCGCGGGGCGGGGGTCGGGGGCGGAGTAGGGGAATCGGAAGGGA |
| mm4_intergenic_Gm5611|Chordc1_chr9_17289422 |
CAGCACCACAAAACCGTCAGCATTCAAACCTGCTGCAGATTTGCCTGTCCAGAACACTATCACAGCATCTGGCAGGCGGG CTGAGCGAGGCCCAGGAAGGAGCTGATAAATGGGAACATGTCCAGTCCTGAGGAAGTGG |
| mm4_intergenic_Fam78a|Ppapdc3_chr2_32088789 |
AGCCTCTGGGGAAAGACAGAGGGGGGCGACAGCAGGCTGGGCGCGGCCTCGGATCCGGTGCGGCACTGAGAGTTCTCCAA GGTGCTAAAATAAACCGAGCGAAGGATCAGG |
| mm4_exon_Mafa_chr15_75747660 |
CTTTTCCTGAGGCGCCCCCGACAGTGCCGGGGCCTCCACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNTGTGCCCGGGCTGGGTGCGCAGAAGCTGGGCGAAGAGG |
| mm4_intergenic_Irs4/Gm15295|Gm15295_chrX_141726006 |
GGCTGTAAACAACCCTCGGAAAATAGCTGTTAATGGTGGTGATAAATTCCTGCAAATCCCAGAGCCGCGGTGACCCCTCT GCCAGGAGCCCTTTCTCTTTTTCCCTACAGCTCACATTTTCCCTTCCGATCGCAGT |
| mm4_intergenic_Dpy30|Spast_chr17_74338740 |
TCCCAGGTCAACAAAGACGGCGGCGTACGCGCGCGCACCAGAGCCATTCACCGAGGACGTGCGCGTGCCCGGCCGCCGCA GCGAGGTGCCAGCGGCCCAGGGGACAGAAGGAAAGGAGGGGCAG |
| mm4_intron_Chst9_chr18_15759900 |
AGCCACAATGGTACCTTGCACGTATCCCGTCCAGAGCCGCGCCCACCGCGGTGATCAGAGGCGCCCGGACTGGGAGTACC GCGCATCCCCCTAGCCCCAGTGCAGTTAC |
| mm4_intron_Sdk2_chr11_113981971 |
GGTCCCCAGAGTTTGAAGGGGGCTGGGCTGTGGTGTGGGGACTGGGCTGTATGCCAAGTGTTCTGTGTCCTTCTGAAAGA TGGAACAGTGCCAGGGCCTGGCTCTCCCTGCTGGCGAGTGGATACAGAGGCAG |
| mm4_intergenic_Rere|Slc45a1_chr4_150627778 |
CACCCTTTCCCCAGTTCTCCCCAGTTAGTCAACAGCAGGATGAGAGAGGCCCAGGGACATTACTAAGAGCCTTGAGTAAG CTGGGATGTTGGTGGCAGAAGGTGGCTAGAG |
| mm4_exon_Fam198a_chr9_121978443 |
CCTCACACTGTTCAGGGACAAGGACCCGTGAGCCCAAGGCTGCCTGGGCCGAGCACACCTTGCTGGAGAACTTCACACAA TGTAAACCTCAGCTGGTGCGCGTCCCTCTCAATGACAGCA |
| mm4_exon_Ahctf1/Gm10518_chr1_179803545 |
CTTCTCGCTCAGTCTCTCGCCGGTCCCCTCAGGCCCCGGGTGCTCCTTCGCCCAATCCCGGCTTCGAACTCGTCCCACCG CCCGCGCCGCGTCCTCCCCGCTGGCGACTCGACTTACTGGGCTGG |
| mm2_exon_Slc12a5_chr2_164997920 |
AGAAACGCAACGGACTCCTCGCCACGGCCGCGCTCTCCCCGCTGCGGGCACCCAGGAAGGCGGAAGCCGGGGAGCCAGGT TTCCCTGCGCTCCCGAGCTGAGAGCCAAGTGCTTTAAGGCCGGCGCTCTCCTTTCCCTTT |
| mm4_exon_Rasgef1b_chr5_99252858 |
CGAACGCCAGAAGAAAGGGAAAACCCGCCGATCCAGTCGGGGTCCTTGTTTATACTGAAACTCCAGCCCGAGCCGCGCAC ACCCCCTTGCTGGGGAAATGCCCGCCTCCTG |
| mm4_exon_Fbxw4_chr19_45582784 |
CTCAGAACAGAAGGCAGGGCTCCTGGACCTGGCTCACCCTGCTGACCAAGAGAAAGAGAAATTTAATAATGTATTGCCAT TAACCACTGTGGCCCA |
| mm4_intergenic_Smim17|Gm20715_chr7_6438636 |
AAAGGTAGATGGGGCCCATGGGGACCCAGGGCCGAGATCAACCCACTGAAGCCTCATTACTGATGGCCAATAATGGGTTC TTGTGAGAGTCAAGTGTGGTCCCAATGTCCTGACCTAGGGAGAGAGCA |
| mm4_intergenic_Ncoa1|Gm22343_chr12_4376002 |
GCAGCTGAGCAATGGAGAGTTGTGATCACAGAACTACCCAGATACAAGGAACAGCAACAGCTGGGTGAGCAAGACCCTGG ACAACAAGAACTCCGAGTGGCAAAAAGAAGTGGTCTGTCAGCACATGCAA |
| mm4_exon_Rell2_chr18_37956354 |
CAGCCCACTAAACCCAGGAGGCGCCCTGGCCCGCGCTCGCCCCCCAGGGCCTCATGTCGGAACCACAGCCTGACCTGGAG CCGCCCCAACATGGGCTGTACATGCTCTTCCTGCTTGTGCT |
| mm4_intergenic_Plcd1|Dlec1_chr9_119100668 |
GCTGCTAGTGACAGGATCCCTCGGTCTGGGGAGACGGTCCCTTACACCAGGGCCACGCCCACTCGGCTGCCAGGGCCACG CCCACTCGGCTGCTACTCTGCTACTCTCCCCACA |
| mm4_intergenic_Plcd1|Dlec1_chr9_119100645 |
GCTGCTAGTGACAGGATCCCTCGGTCTGGGGAGACGGTCCCTTACACCAGGGCCACGCCCACTCGGCTGCCAGGGCCACG CCCACTCGGCTGCTACTCTGCTAC |
| mm3_exon_Tmem41b_chr7_109986350 |
CCAGTAACCGCGAGACAAGTCAGGGCCGACCCCGCCCCCTCCGGGGCCCCGCCCACCTCGCTGGTGTAGGGCGCATGAGC AATATCGCATTGGCAAGTCATGGGTCTGCGGCCTAAGGTCCGACTTCTCCGC |
| mm4_intron_Adamts15_chr9_30918904 |
ACGACTCTGGCTCTGACTCTCGGCAGAGCCTCGATCTGTTCCTGGGCTGACCTCACCCTGCTGTTATGAGCCTGGAGGCT GGCTGCTTTGCAAAATC |
| mm4_exon_Cdk5rap3_chr11_96911434 |
CTGGTCTCAGGAAAGCCAGGAAAATCAAAGGGCCTTTGGGTCCGCCTACCCGAGCCTCGCTCACCCAGCTTCCCTTACTC TTCACTATGGGAAGTCAGGTCAGGGCTCGGGCTCCGTGTGGATGGGAAACAGCTCC |
| mm4_intergenic_mmu-mir-7031|Sh2b3_chr5_121820781 |
ACAAATTCCATGCTCGTGGCACCTGACTGCCAGGCCCGGGCTGACTCCGCTGGCCCTCTTTACTCAAGGTTCCCTATGGG TCAAAAAGACTTGGTACAAAGGCCTCTTGGGAAGTGCTCTGTCCC |
| mm4_intergenic_Cep68|Rpl12-ps2_chr11_20249605 |
AGCTTCTTTCTCCGCTGTCCGGACGCGCGTGCCAGCTGGGTGTTCGCGGTCCTGGCGTCCACGCAGGCCCACGCGGATAG CAAGGCGCGCTCTCCGCGGATCGAAGGCTTGCGGTGTTT |
| mm4_intron_Ano6_chr15_95919592 |
AGTGCACAGACCAAGACAGAAATAAAAGCAGCTCATAGAAACCCACATCACAGTGCTGCCCGGGCCTCCCTCACCACTCT GGCGGGAACAGTCTTTTCCTG |
| mm4_intron_3110035E14Rik_chr1_9613181 |
TGCAACAACCACCTTAGCCTTTAGCACCCTTTTCTGACTTCCTGCCACCCTGGTCCAGGCTCACCTCGCTGTGGCCATTT ATTCATTCCTTACAGTTCCCTAGTGGACATTAGGTGGGTGC |
| mm4_exon_Ebf1_chr11_44620410 |
CGGGACACAGTCTGGCTTAGCCATCTAACTCTGCGTCTTTGGTCGCAGCGGGGTGGGTCTGGCCCGGGCTCACTTTGAGA AGCAGCCGCCTTCTAA |
| mm3_exon_Gnai1_chr5_18360035 |
CTGTTGAGGGACTGGGATGCCGCGCTCGCTCACCGCTGTCCCCCGGCCCGGGCCCCGTCCCCACGGCCGCGCTCACCCAG CAGCAGCAGCTTGACCTCCCG |
| mm4_exon_Crocc_chr4_141021778 |
ACACTCTCTTCCTGCAGTGCCAGCTCTGCCTGGGCCCCGCTCAGCCGCCTGTCTGCACTGCGCCAGGCTGTGGCCACCAC CCAGTCCCATCAGGCAAGCCAGGAAGGCCCAATCTGTACCCCACCCTCTCC |
| mm4_intergenic_Gm8325|P2ry1_chr3_61002742 |
CTTGCACGGCGGAGACACGCCCCTTGCCTCCCCGCGGAGCACAGACTCGCCCGGGCTCCTAGGCTCCGCCCACCCCGCTG GGAGCCTGGCTTTCTCTCCTG |
| mm4_intron_Camk2b_chr11_5988780 |
TCCTTTAGCCCTCAGGGTGATGAAGCGACCAGCTCTGTACCCAGGGCCAAGCTCAACCCTCTGAGCTGTGCCAGCATCTG GAATCCTCTGGTCTGGACTTGCAAACAGGCATTTGGGGGTTTCTCCTGCTTCCTC |
| mm4_exon_Akap8l_chr17_32332462 |
GGCTCAAATTCCAGGCAAGCAGCCCCGGGCCCAGCTCACCCTGCGGTTTCGGTTGTGATCCATCGTCTTGAGATGCTTTT GGATGATTCCAAACTGCATGGGGATGAAAAGGTCACAGGCTGCA |
| mm4_exon_Cry2_chr2_92413634 |
TCACAGGGTACAGGCTAGCTTGCACTCACCCGGACCCCGCTCTCCCAGCTGACCCAGAGGTCCCCGCGGGTGAGGAAGCA GGCCACAGCGTGCCGGGCCAGGTGGTGGATCCAGCCCTCCTGCCTCAGTTGGGTCATGA |
| mm4_exon_Cacna1a_chr8_84639639 |
CAGGCTGCCCAATGGTTACTANNNNNNNNNNNNNNNNNNNNNNNNNNNNACAGCCCGCAGGGGCGCGCACGACGCGTACA GCGAGAGCGAGGACGACTGGTGCTAAG |
| mm4_exon_Epha5_chr5_84417176 |
GCTGGCAGAGGGAAGTCAAATCGCTGCGGGCTGAGCTCGGCCGGGGGCGGTGCGACCGGCGGAGCGGGCTGGAGCGCGGG CCCGAGAGCGCAGCAGAG |
| mm3_exon_Hus1_chr11_9010980 |
GCAAAAGGAAGGTCCCTGGTAGCCGTGGCTCTTCTCAGGGCCGCCCTCACCCCGCCCGTCCTCTCACAAGGGCCGCACTC TCCGCTCGCTCCCCGCAGCCCTGCCAGCTCACGTGTGAAATG |
| mm4_exon_Lrrc59_chr11_94629796 |
ACGGAAGTGGTCGTCGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTAAGGCGCAGGCGTGG |
| mm4_intergenic_Gm15684|Crispld2_chr8_120046608 |
GCTACTCAGTGGACAGAGCCCTGGGAGCTGTTCAGCCTGAAACCCAAGGGGCGGAAAGCACTTTCGAGAAACCAGCTGCT GGGTGAGCCTGGCCCTGGGCAGCTGCCCCCCACCCCCCATCTCTCTGCTTGGTCTTGGCA |
| mm4_intergenic_Gm5903|Ate1_chr7_130369514 |
TTGGGGAATCCTGGTTCTGCCGGGTGTAAAGGCTGGAACAGAACGCAAGAGGCAGCATTGTGGCTTCTCTAGGAGGTGGC GTCCTGGGCTCCCCTCACCCCGCAGCCCCTCACCACCAAGGTGAGCAAAGCAT |
| mm4_intergenic_n-R5s86|Wdr72_chr9_74110264 |
TATAGGAGGGCGGTGGGAAGCAGGCAGACCAGAGCCAGCTGGGTCAGCGCCGGCCTGGGCGGGGCAGACAGAGGCCGGTG GGGGCGGATCCGACCTGAGGCCTCTGAGCCCGGCCCTGTCACCTGTGGGCTTGGTTC |
| mm4_exon_Itfg1_chr8_85840690 |
CCTCAGCCCCAAAGAGCTCGGCCGTGACGTTGTGCAGAGCCCGCGCCGGGGCAGGCCCAACGCTCAGCAGCGCGAGCCCC AAGAACAGCGGGGCCAGCACCGCCCTGGCGCTAGGAAGCCGGCCTGCC |
| mm4_exon_Gpr88_chr3_116251658 |
GAACTCCTCGTTCCTCCACGTGTAGAGCAGCGGGTTGAGCGCCGACAGGGCGCAGCACAGGAGCCAGCTGGCCGCCTGCA CGCCCCAGGGCACCGGGAGGGAGAAGCCACTGGCCAGGCTCACCCACACCAG |
| mm4_exon_D030028A08Rik/Sp2_chr11_96954360 |
GGGACACAGCCACAGAACAGGAGTGGGGTGAGGGCTGCCCAGGGCAGTCGTGGGCACCTGTCCATCATGGACCCAGGGCC AATCAGATGGGAGGTGTTCCATCTTCAGGGCCTCC |
| mm3_intron_Mfsd6_chr1_52726948 |
CTGCACCCCGAGATGACAGGCGGCACCCGGGAGGGCGGGATAGGCTCGGANNNNNNNNNNNNNNNNNNNNNNNACCCCGC AGCAGGTGCACGGAGGAGGAT |
| mm2_intron_Ephb2_chr4_136835508 |
GCGCAGTGGTAAGACCCCCCAGCCCTGGGCGGCGCTCACCCGGCTGGCTACAAAGCCTCGGCAAGATGGTCCCTTCGCCT CCTCCTGGCACAGTCAATCA |