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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| mm0_exon_Pop5_chr5_115238096_F | TCGTCGGCAGCGTCTGCGGTTCAAGCACAGGTAA | 60.1 | 1.00 |
| mm0_exon_Pop5_chr5_115238096_R | GTCTCGTGGGCTCGGCTCGCACAGAAGGTACCTGC | 60.7 | 1.00 |
| mm2_exon_Cyth3_chr5_143622510_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm2_exon_Cyth3_chr5_143622510_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm3_intron_Sbno2_chr10_80060517_F | TCGTCGGCAGCGTCCCGTCTGGCCCATGATTGTA | 59.8 | 0.29 |
| mm3_intron_Sbno2_chr10_80060517_R | GTCTCGTGGGCTCGGTGCCATGTGTGTGTGCAAAG | 59.8 | 0.29 |
| mm4_intron_Kit_chr5_75575154_F | TCGTCGGCAGCGTCGGAAAGAGCGGCAGACAAGA | 60.3 | 0.28 |
| mm4_intron_Kit_chr5_75575154_R | GTCTCGTGGGCTCGGGGCATGGGAAAAGCCAACAG | 60.0 | 0.28 |
| mm4_intergenic_Gm24150|Gm17359_chr3_79286909_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm24150|Gm17359_chr3_79286909_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_9430029A11Rik|Plxna4_chr6_32492004_F | TCGTCGGCAGCGTCAGCCAACACCATCCTGACTT | 59.2 | 0.22 |
| mm4_intergenic_9430029A11Rik|Plxna4_chr6_32492004_R | GTCTCGTGGGCTCGGGATGAGCTTGCATCCCCTGG | 60.8 | 0.22 |
| mm4_exon_Sdc1_chr12_8771700_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_exon_Sdc1_chr12_8771700_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_exon_Bnip2_chr9_69989688_F | TCGTCGGCAGCGTCGAGGGGCAAAGAGGACGAG | 59.7 | 0.22 |
| mm4_exon_Bnip2_chr9_69989688_R | GTCTCGTGGGCTCGGGATCCTCCCGCAGCCCTC | 61.9 | 0.22 |
| mm3_exon_Tet1_chr10_62878129_F | TCGTCGGCAGCGTCACAAATTCTTAGGGGCGGGG | 60.0 | 0.21 |
| mm3_exon_Tet1_chr10_62878129_R | GTCTCGTGGGCTCGGAGGAAATGCGAGGTGCTCAA | 59.9 | 0.21 |
| mm3_intergenic_Ccdc134|Srebf2_chr15_82147201_F | TCGTCGGCAGCGTCCTAATGCAAGCCTGGTCCCA | 60.0 | 0.21 |
| mm3_intergenic_Ccdc134|Srebf2_chr15_82147201_R | GTCTCGTGGGCTCGGCATGTTTGAGCTGCGCCC | 59.8 | 0.21 |
| mm4_intron_D13Ertd608e_chr13_119836875_F | TCGTCGGCAGCGTCGGACGGGATCTGATTCTGCC | 60.2 | 0.21 |
| mm4_intron_D13Ertd608e_chr13_119836875_R | GTCTCGTGGGCTCGGCCCTGCATACTCACACCTGG | 60.1 | 0.21 |
| mm4_intergenic_Gm21762|Gm21731_chr13_120224461_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm21762|Gm21731_chr13_120224461_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Chrdl2|Pold3_chr7_100042171_F | TCGTCGGCAGCGTCCAGCGCAGGGATCACTCTAG | 59.9 | 0.20 |
| mm4_intergenic_Chrdl2|Pold3_chr7_100042171_R | GTCTCGTGGGCTCGGAGTCACGAATCAAAGCCCGT | 59.9 | 0.20 |
| mm4_intron_Triobp_chr15_78982933_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Triobp_chr15_78982933_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_exon_E2f4_chr8_105299038_F | TCGTCGGCAGCGTCCACCTTGGCCTACGTGACTC | 60.3 | 0.18 |
| mm4_exon_E2f4_chr8_105299038_R | GTCTCGTGGGCTCGGCCATGACTTAGCTCAGCCCC | 60.1 | 0.18 |
| mm4_intron_Fkbp4_chr6_128438254_F | TCGTCGGCAGCGTCGATCGAGTTGGCGTTGCAGT | 61.2 | 0.17 |
| mm4_intron_Fkbp4_chr6_128438254_R | GTCTCGTGGGCTCGGGATTCGTCTTCCCTGCGCAG | 61.4 | 0.17 |
| mm4_intergenic_Cd4|Lag3_chr6_124901293_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Cd4|Lag3_chr6_124901293_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_exon_Wfdc2_chr2_164563495_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_exon_Wfdc2_chr2_164563495_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_exon_Elac2_chr11_64979221_F | TCGTCGGCAGCGTCCATCCAAAGACCCACTGCGA | 60.3 | 0.16 |
| mm4_exon_Elac2_chr11_64979221_R | GTCTCGTGGGCTCGGCCTGCAGGTACACGGTGTTC | 60.9 | 0.16 |
| mm4_intron_Grb10_chr11_12036652_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Grb10_chr11_12036652_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Wtip_chr7_34133176_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Wtip_chr7_34133176_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm2_intergenic_Mir181c|Gm26887_chr8_84183775_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm2_intergenic_Mir181c|Gm26887_chr8_84183775_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_E130114P18Rik|Gm12676_chr4_97751384_F | TCGTCGGCAGCGTCCGTGCTTGAACCCCAATCCT | 60.6 | 0.14 |
| mm4_intergenic_E130114P18Rik|Gm12676_chr4_97751384_R | GTCTCGTGGGCTCGGCACACTGTCCTCAGCCCTTT | 59.8 | 0.14 |
| mm4_intron_Suv420h1_chr19_3768821_F | TCGTCGGCAGCGTCCACGGCCTGCAGTGTTGG | 61.6 | 0.14 |
| mm4_intron_Suv420h1_chr19_3768821_R | GTCTCGTGGGCTCGGCGCCCATTGGAGAAGAGGAA | 59.7 | 0.14 |
| mm4_exon_Nos1ap_chr1_170589733_F | TCGTCGGCAGCGTCGCCAGAAGCGGGACTAGAC | 59.8 | 0.14 |
| mm4_exon_Nos1ap_chr1_170589733_R | GTCTCGTGGGCTCGGGTCTCCGTGACAGGCACC | 60.0 | 0.14 |
| mm4_intergenic_Cebpa|Slc7a10_chr7_35138629_F | TCGTCGGCAGCGTCTTTAGACATAAATGGTTCCCTTCAC | 57.3 | 0.14 |
| mm4_intergenic_Cebpa|Slc7a10_chr7_35138629_R | GTCTCGTGGGCTCGGCTCCCCAAGTCATCCCACAC | 60.0 | 0.14 |
| mm4_intron_Cmas_chr6_142757037_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Cmas_chr6_142757037_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gnb4|Actl6a_chr3_32616645_F | TCGTCGGCAGCGTCGAGAGGGTCTGGGAACGC | 59.4 | 0.13 |
| mm4_intergenic_Gnb4|Actl6a_chr3_32616645_R | GTCTCGTGGGCTCGGCAATCAGCCTCCACCCAGG | 60.0 | 0.13 |
| mm4_exon_Zfp131_chr13_119790756_F | TCGTCGGCAGCGTCACAACGACTCCCCGGGTC | 61.3 | 0.13 |
| mm4_exon_Zfp131_chr13_119790756_R | GTCTCGTGGGCTCGGGTAAGTGACGCGCAGGCC | 61.4 | 0.13 |
| mm4_intron_Jam3_chr9_27155217_F | TCGTCGGCAGCGTCGAGCCGAAACCGCTATTCCA | 60.4 | 0.12 |
| mm4_intron_Jam3_chr9_27155217_R | GTCTCGTGGGCTCGGTGCCTGACTTCTTCCTGCTG | 59.9 | 0.12 |
| mm4_intergenic_Nkx6-1|Cds1_chr5_101665325_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Nkx6-1|Cds1_chr5_101665325_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Pth1r_chr9_110732780_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Pth1r_chr9_110732780_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Dennd4b|Gatad2b_chr3_90293275_F | TCGTCGGCAGCGTCTGTCTGTACCGCAGTGTCTG | 59.6 | 0.10 |
| mm4_intergenic_Dennd4b|Gatad2b_chr3_90293275_R | GTCTCGTGGGCTCGGGCACAGTGGGATCCAGACC | 60.0 | 0.10 |
| mm4_intergenic_A330074K22Rik|Gse1_chr8_120228708_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_A330074K22Rik|Gse1_chr8_120228708_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_St6galnac5|St6galnac3_chr3_153027419_F | TCGTCGGCAGCGTCTGGAGCAGGCTATCCCAGTT | 60.6 | 0.09 |
| mm4_intergenic_St6galnac5|St6galnac3_chr3_153027419_R | GTCTCGTGGGCTCGGACTTGAGGCCTTAAGAGTCTGG | 59.4 | 0.09 |
| mm4_intron_Ephb2_chr4_136835502_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Ephb2_chr4_136835502_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Gm26560/Drd5_chr5_38319529_F | TCGTCGGCAGCGTCAAAGGATCCCAAGCAGTGCA | 59.8 | 0.08 |
| mm4_exon_Gm26560/Drd5_chr5_38319529_R | GTCTCGTGGGCTCGGGATCCCAACTTCGCCCCTG | 60.4 | 0.08 |
| mm3_exon_Kdelr3_chr15_79516639_F | TCGTCGGCAGCGTCTGCTCCTGGTGAAGATCTGG | 59.0 | 0.08 |
| mm3_exon_Kdelr3_chr15_79516639_R | GTCTCGTGGGCTCGGGGCTTCCCTCTGAGCCTAAG | 59.5 | 0.08 |
| mm4_exon_Pde3b_chr7_114415943_F | TCGTCGGCAGCGTCGCCTGTGCCTTCTTCTTCCT | 59.9 | 0.07 |
| mm4_exon_Pde3b_chr7_114415943_R | GTCTCGTGGGCTCGGGAAGTCGCCCAGGTAGCAG | 60.1 | 0.07 |
| mm4_intron_Vkorc1l1_chr5_129942458_F | TCGTCGGCAGCGTCAGCTCCGGCCTGAGAGAG | 60.4 | 0.07 |
| mm4_intron_Vkorc1l1_chr5_129942458_R | GTCTCGTGGGCTCGGTGCACTGTGCCACCTCATC | 60.3 | 0.07 |
| mm4_intron_Dtx1_chr5_120711190_F | TCGTCGGCAGCGTCGGAGAACAGCGGGCATCATA | 59.8 | 0.07 |
| mm4_intron_Dtx1_chr5_120711190_R | GTCTCGTGGGCTCGGTTCTGCATCTCCACATCCCG | 59.8 | 0.07 |
| mm4_exon_Gpr146_chr5_139380742_F | TCGTCGGCAGCGTCCCTCCTGCAAGCCCAGCT | 62.0 | 0.07 |
| mm4_exon_Gpr146_chr5_139380742_R | GTCTCGTGGGCTCGGTGGAACAGAGCATTAGGGGC | 59.7 | 0.07 |
| mm2_intron_Lhx4_chr1_155741355_F | TCGTCGGCAGCGTCTCGAACTGCCGAAACCAAGA | 59.8 | 0.07 |
| mm2_intron_Lhx4_chr1_155741355_R | GTCTCGTGGGCTCGGACAACTTTACCCCAGCTGGC | 60.5 | 0.07 |
| mm4_exon_Hdac5_chr11_102206816_F | TCGTCGGCAGCGTCCGGAAGGGAGGGCAGAGG | 61.4 | 0.07 |
| mm4_exon_Hdac5_chr11_102206816_R | GTCTCGTGGGCTCGGCGAGGTCCAGCTGCAGAAG | 60.4 | 0.07 |
| mm4_exon_Dlgap3_chr4_127169310_F | TCGTCGGCAGCGTCCAGTGTCGGGTCCGGGAG | 61.7 | 0.06 |
| mm4_exon_Dlgap3_chr4_127169310_R | GTCTCGTGGGCTCGGCCTCCCGGTTGCTTACCTG | 60.0 | 0.06 |
| mm4_intron_Zp1_chr19_10919949_F | TCGTCGGCAGCGTCCATTTGGGGACACCTGCTCA | 60.2 | 0.06 |
| mm4_intron_Zp1_chr19_10919949_R | GTCTCGTGGGCTCGGCCCTTCCCACACACACACTT | 60.1 | 0.06 |
| mm4_intergenic_Tlr6|Fam114a1_chr5_64970021_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Tlr6|Fam114a1_chr5_64970021_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Sgpl1_chr10_61147147_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Sgpl1_chr10_61147147_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Rasgef1c|Rnf130_chr11_50025310_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Rasgef1c|Rnf130_chr11_50025310_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Prokr2_chr2_132385305_F | TCGTCGGCAGCGTCGGTACTCACTTGCCTCGGTG | 60.3 | 0.06 |
| mm4_exon_Prokr2_chr2_132385305_R | GTCTCGTGGGCTCGGGCAAGCCTCAACCAGAGCTA | 60.0 | 0.06 |
| mm4_intron_Kat6a_chr8_22859823_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Kat6a_chr8_22859823_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Tmem240_chr4_155735503_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Tmem240_chr4_155735503_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Gm26801_chr1_177442457_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Gm26801_chr1_177442457_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Scn5a_chr9_119512904_F | TCGTCGGCAGCGTCAAAGGGAAGCAGACTCCAGC | 59.9 | 0.05 |
| mm3_intron_Scn5a_chr9_119512904_R | GTCTCGTGGGCTCGGCCCAGAGGACTGCTTCACTG | 60.0 | 0.05 |
| mm4_intergenic_Rps6-ps2|mmu-mir-8110_chr8_88918804_F | TCGTCGGCAGCGTCGGTCGATTAAGTCACAGCAAGC | 59.9 | 0.05 |
| mm4_intergenic_Rps6-ps2|mmu-mir-8110_chr8_88918804_R | GTCTCGTGGGCTCGGTGTGATCGCTATGTGACAAACG | 59.3 | 0.05 |
| mm4_exon_Aak1_chr6_86849654_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Aak1_chr6_86849654_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Slc6a6_chr6_91684339_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Slc6a6_chr6_91684339_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Ppard_chr17_28232883_F | TCGTCGGCAGCGTCAAGTTTTGGCAGGAGCTGGG | 60.8 | 0.04 |
| mm4_intron_Ppard_chr17_28232883_R | GTCTCGTGGGCTCGGCCCTGACCCCACCATCCA | 60.6 | 0.04 |
| mm4_intron_Eml1_chr12_108509526_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Eml1_chr12_108509526_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Creb3l1_chr2_92024240_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Creb3l1_chr2_92024240_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Smarcad1_chr6_65042997_F | TCGTCGGCAGCGTCGTGAGGACCTCGTGCGAG | 59.8 | 0.04 |
| mm4_intron_Smarcad1_chr6_65042997_R | GTCTCGTGGGCTCGGGCTGTGCTTTGTTAGGCCAC | 60.0 | 0.04 |
| mm4_exon_A830092H15Rik_chr3_26331530_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_A830092H15Rik_chr3_26331530_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_D17Wsu92e|Snrpc_chr17_27820643_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_D17Wsu92e|Snrpc_chr17_27820643_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_AC121131.1_chr8_19535372_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_AC121131.1_chr8_19535372_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Cd101_chr3_101014816_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Cd101_chr3_101014816_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Trpv4_chr5_114650152_F | TCGTCGGCAGCGTCGTGCCTGGAATGGGGTAGAC | 60.1 | 0.03 |
| mm4_intron_Trpv4_chr5_114650152_R | GTCTCGTGGGCTCGGAGGGTCTGTCTCCTGCTGTA | 59.5 | 0.03 |
| mm4_exon_Cbr1_chr16_93608063_F | TCGTCGGCAGCGTCCTCGTGACCTGTGTCGGAAA | 59.9 | 0.03 |
| mm4_exon_Cbr1_chr16_93608063_R | GTCTCGTGGGCTCGGTTGTCGATGTCCAGCTGGTG | 60.3 | 0.03 |
| mm4_exon_Stox2_chr8_47288355_F | TCGTCGGCAGCGTCGGGCTTCCAGCTTCCAGATT | 60.0 | 0.03 |
| mm4_exon_Stox2_chr8_47288355_R | GTCTCGTGGGCTCGGCATTGTCTGCGCTCCCATTG | 59.9 | 0.03 |
| mm4_intergenic_Gm25144|Gm23147_chr3_6137518_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25144|Gm23147_chr3_6137518_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Snrk_chr9_122166947_F | TCGTCGGCAGCGTCCCAGAGCAAGCCTAGCAGC | 60.8 | 0.03 |
| mm4_exon_Snrk_chr9_122166947_R | GTCTCGTGGGCTCGGCTTGAGGACCCTGCTGTACC | 59.7 | 0.03 |
| mm4_intergenic_Acsbg1|Dnaja4_chr9_54691650_F | TCGTCGGCAGCGTCTAACTGTCTCCGGGAAGCAG | 59.1 | 0.02 |
| mm4_intergenic_Acsbg1|Dnaja4_chr9_54691650_R | GTCTCGTGGGCTCGGAAGGAGTTTGAGGAGCGAGC | 60.0 | 0.02 |
| mm4_exon_Tmem230_chr2_132247700_F | TCGTCGGCAGCGTCTAACTGTCTCCGGGAAGCAG | 59.1 | 0.02 |
| mm4_exon_Tmem230_chr2_132247700_R | GTCTCGTGGGCTCGGTTGAGCGAGCCTTGCGAG | 60.4 | 0.02 |
| mm4_intergenic_Gm25456|BC048562_chr9_108431846_F | TCGTCGGCAGCGTCCACCTCGAGTTCTCACCTCG | 59.8 | 0.02 |
| mm4_intergenic_Gm25456|BC048562_chr9_108431846_R | GTCTCGTGGGCTCGGCGGGGTCAAGAATCGGCATT | 60.7 | 0.02 |
| mm4_intron_Ubqln4_chr3_88554086_F | TCGTCGGCAGCGTCGGCATACAGGCGGGAGAAG | 60.2 | 0.02 |
| mm4_intron_Ubqln4_chr3_88554086_R | GTCTCGTGGGCTCGGGTGTCCCTTCCTCAAGCTCC | 60.0 | 0.02 |
| mm4_exon_Rrm2_chr12_24708444_F | TCGTCGGCAGCGTCGACTCACCCTGGCTGACAAG | 60.3 | 0.02 |
| mm4_exon_Rrm2_chr12_24708444_R | GTCTCGTGGGCTCGGCCAGTGCACATCCAACCCA | 60.2 | 0.02 |
| mm4_exon_Shank3_chr15_89548166_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Shank3_chr15_89548166_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Zfp69|Smap2_chr4_120951722_F | TCGTCGGCAGCGTCCTCTAGCCCGGTTCGTGTC | 59.8 | 0.02 |
| mm4_intergenic_Zfp69|Smap2_chr4_120951722_R | GTCTCGTGGGCTCGGGCTTCCGGGATCTGGGTC | 59.4 | 0.02 |
| mm4_exon_Zbtb14_chr17_69383268_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Zbtb14_chr17_69383268_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Utp15|Ankra2_chr13_98263093_F | TCGTCGGCAGCGTCCAGGCGCAGAAGGTTTACGA | 60.6 | 0.02 |
| mm4_intergenic_Utp15|Ankra2_chr13_98263093_R | GTCTCGTGGGCTCGGGTAGCCCATCCTCTCGGAGA | 60.1 | 0.02 |
| mm4_intron_Myh14_chr7_44639141_F | TCGTCGGCAGCGTCAGGGTGGTGAAGGATGGGAT | 60.2 | 0.01 |
| mm4_intron_Myh14_chr7_44639141_R | GTCTCGTGGGCTCGGATCGCGGGCTTTGAGATCTT | 59.8 | 0.01 |
| mm4_exon_Sptbn4_chr7_27404769_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Sptbn4_chr7_27404769_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Rps2-ps6|Tsc22d1_chr14_76414152_F | TCGTCGGCAGCGTCACCGCTCTGCACAAAAGGTA | 59.8 | 0.01 |
| mm4_intergenic_Rps2-ps6|Tsc22d1_chr14_76414152_R | GTCTCGTGGGCTCGGCCTCCTTCTCCAAGCAGCAA | 59.9 | 0.01 |
| mm4_intron_Bves_chr10_45336061_F | TCGTCGGCAGCGTCCGCTGACACTGCTGTGAGA | 60.0 | 0.01 |
| mm4_intron_Bves_chr10_45336061_R | GTCTCGTGGGCTCGGCAGCCACCCGTTTCTCTAGG | 60.1 | 0.01 |
| mm4_exon_Clk3_chr9_57765812_F | TCGTCGGCAGCGTCCCTTGAACACCTCAGAGCGT | 59.9 | 0.01 |
| mm4_exon_Clk3_chr9_57765812_R | GTCTCGTGGGCTCGGCTGAGTGACTCGAGTGGCAG | 60.1 | 0.01 |
| mm4_exon_Ism1_chr2_139678530_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Ism1_chr2_139678530_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Klf4_chr4_55532316_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Klf4_chr4_55532316_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Rhou_chr8_123654215_F | TCGTCGGCAGCGTCCCTACGGCCTTCGACAACTT | 60.0 | 0.00 |
| mm4_exon_Rhou_chr8_123654215_R | GTCTCGTGGGCTCGGGAATCCCGGAGCCAGACC | 59.4 | 0.00 |
| mm4_intron_Cisd2_chr3_135423180_F | TCGTCGGCAGCGTCTCACGTGCCAAGGCCTGA | 61.5 | 0.00 |
| mm4_intron_Cisd2_chr3_135423180_R | GTCTCGTGGGCTCGGCGCCTCACAGGTAACCCTC | 59.7 | 0.00 |
| mm4_intergenic_Cenpb|Cdc25b/Spef1_chr2_131180054_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cenpb|Cdc25b/Spef1_chr2_131180054_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Ndufa11_chr17_56717941_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Ndufa11_chr17_56717941_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hivep1_chr13_42052257_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hivep1_chr13_42052257_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ptpn2_chr18_67724318_F | TCGTCGGCAGCGTCTCGGTGCTTCAGGTCTAAGC | 59.7 | 0.00 |
| mm4_intron_Ptpn2_chr18_67724318_R | GTCTCGTGGGCTCGGGCTCCGTGTCACCTCCCC | 62.0 | 0.00 |
| mm4_intron_Phip_chr9_82974752_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Phip_chr9_82974752_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hmgb3_chrX_71556754_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hmgb3_chrX_71556754_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Fam154b_chr7_82648333_F | TCGTCGGCAGCGTCGGTTCAGGCAAGGGAGTCC | 60.0 | 0.00 |
| mm4_intron_Fam154b_chr7_82648333_R | GTCTCGTGGGCTCGGGCAGAGCATGAGGAACTGGT | 60.0 | 0.00 |
| mm4_intergenic_Ppp2r3a|Gm24638_chr9_101251878_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ppp2r3a|Gm24638_chr9_101251878_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Kcnma1|Gm6158_chr14_24004404_F | TCGTCGGCAGCGTCATGAGGGAGGGTGGCTCC | 60.3 | 0.00 |
| mm4_intergenic_Kcnma1|Gm6158_chr14_24004404_R | GTCTCGTGGGCTCGGGAGCTTCGGGAGGCACAG | 60.1 | 0.00 |
| mm4_intergenic_Gpr25/Gm26781|Camsap2_chr1_136267527_F | TCGTCGGCAGCGTCAGATGCTCCTGTCTGCTGTG | 59.7 | 0.00 |
| mm4_intergenic_Gpr25/Gm26781|Camsap2_chr1_136267527_R | GTCTCGTGGGCTCGGAGTGTATCAGCAGAGGGAGGA | 59.7 | 0.00 |
| mm4_intergenic_Gm7292|Gm6253_chr6_117779583_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm7292|Gm6253_chr6_117779583_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Smarca5_chr8_80738900_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Smarca5_chr8_80738900_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Slc6a11_chr6_114131516_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Slc6a11_chr6_114131516_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Slc2a13_chr15_91572727_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Slc2a13_chr15_91572727_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Otud5_chrX_7842484_F | TCGTCGGCAGCGTCCAGGCTTCTCCACCTCCTTG | 60.0 | 0.00 |
| mm4_exon_Otud5_chrX_7842484_R | GTCTCGTGGGCTCGGCCCACGGTACCACCCACG | 62.3 | 0.00 |
| mm4_exon_Kbtbd2_chr6_56797436_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Kbtbd2_chr6_56797436_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Gm13111_chr4_154637649_F | TCGTCGGCAGCGTCCTCCAAGCTCTGCCACCG | 60.4 | 0.00 |
| mm4_exon_Gm13111_chr4_154637649_R | GTCTCGTGGGCTCGGGAGACGCCCAGGACAAGATG | 60.4 | 0.00 |
| mm4_exon_Dgcr6_chr16_18066655_F | TCGTCGGCAGCGTCCAGCACTACAGAGCCTGGTC | 60.1 | 0.00 |
| mm4_exon_Dgcr6_chr16_18066655_R | GTCTCGTGGGCTCGGCTGAAAGGAGCTGCGGGTAG | 60.4 | 0.00 |
| mm4_exon_B4galnt1_chr10_127166221_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_B4galnt1_chr10_127166221_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Atxn2_chr5_121711806_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Atxn2_chr5_121711806_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Asmt_chrX_170677138_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Asmt_chrX_170677138_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Agps_chr2_75832334_F | TCGTCGGCAGCGTCTTTCACCCGAGCCCAGCG | 62.6 | 0.00 |
| mm4_exon_Agps_chr2_75832334_R | GTCTCGTGGGCTCGGATGGCCCGACAGAACCCG | 62.0 | 0.00 |
| mm3_exon_Tuba3a_chr6_125285945_F | TCGTCGGCAGCGTCCTCAGAGGAGACGGAGGTCA | 60.0 | 0.00 |
| mm3_exon_Tuba3a_chr6_125285945_R | GTCTCGTGGGCTCGGGGGTCTCCGGAGTTCAGCAT | 61.6 | 0.00 |
| mm3_exon_Lin7c_chr2_109890920_F | TCGTCGGCAGCGTCCTCCGAAGAGAAGATGGCGG | 60.2 | 0.00 |
| mm3_exon_Lin7c_chr2_109890920_R | GTCTCGTGGGCTCGGGAGCGGTTCGTAGTCTCGAC | 60.2 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm0_exon_Pop5_chr5_115238096 |
TGCGGTTCAAGCACAGGTAAGCAGCGCTGTCCCCGCGCCGTCCCCGCGACCCGGGCCGCGCTCACCCCGCTGTCGCCGCA GGTACCTTCTGTGCGAG |
| mm3_intron_Sbno2_chr10_80060517 |
CCGTCTGGCCCATGATTGTAGCAAGGACGCGGCTGAGCGCACGGGCGCCATACTGTGGGGCAGACCAGGTGAGTGAGGCC CGGGTAGAGGGAGGCCTTTGCACACACACATGGCA |
| mm4_intron_Kit_chr5_75575154 |
GGAAAGAGCGGCAGACAAGAGGACTGCACCCTCTGTGGGCGCAGCCCGGGTCCGGGAGGGGTGCCACCTGGGTGCGTCCG GAGTGGTAGCTGTTGGCTTTTCCCATGCC |
| mm4_intergenic_9430029A11Rik|Plxna4_chr6_32492004 |
AGCCAACACCATCCTGACTTGCTCAATCATGAGAAGCTGTGCTCTGAATCACACCTAGTGTGAGACACCATGAGCTACCT CAACCTGGGCCTGGCTCACCCCCATAGACCCAGGGGATGCAAGCTCATC |
| mm4_exon_Bnip2_chr9_69989688 |
GAGGGGCAAAGAGGACGAGGGGGCCGCGGCTTCCTCCGGGGTGAGCACGGCTTGGGTAGCGGGCCGGGCTACGGCACAGC CGGAGGGCTGCGGGAGGATC |
| mm3_exon_Tet1_chr10_62878129 |
ACAAATTCTTAGGGGCGGGGACTGATGCTGCGCCAAACACAGGAGATCCCAGCCCCGGGCTGCGCTCACCTGTGCCTGTG AGGTGGCCTCTGGCTTCTTTTTGAGCACCTCGCATTTCCT |
| mm3_intergenic_Ccdc134|Srebf2_chr15_82147201 |
CTAATGCAAGCCTGGTCCCATTGACAACAAACTTGGGGGTGCGGAGGCTCGGGGCGGGGCTGCAGTNNNNNNNNNNNNNN NNNNNNNAATTCTGCCCCGCCCCTTCCGTGGAGTCGGGGCGCAGCTCAAACATG |
| mm4_intron_D13Ertd608e_chr13_119836875 |
GGACGGGATCTGATTCTGCCGGGCATGCTGAAGTGGGATTCTGTGCGAACCCACGATCCGGACCGCGTTCGCCTAGACGC TGACAGAAATGCTTGGCGTCCAGGTGTGAGTATGCAGGG |
| mm4_intergenic_Chrdl2|Pold3_chr7_100042171 |
CAGCGCAGGGATCACTCTAGAGGCAAGACTCGAACTCCTCCACTGCCCGCTGCTGCCAGCAGGGTGCTCGCGGCCCGGGA CAGGGGGCGGGGGTCGGGGCACCGGCGCAGAAGAACACGGGCTTTGATTCGTGACT |
| mm4_exon_E2f4_chr8_105299038 |
CACCTTGGCCTACGTGACTCATGAAGACATCTGCAGATGCTTTGCTGGTGAGCGGAGCCTGGGTAGAGGGAGGTGGGGGG TGGGGCTGAGCTAAGTCATGG |
| mm4_intron_Fkbp4_chr6_128438254 |
GATCGAGTTGGCGTTGCAGTGGGGGCTTCTTCCCGGACCCGGGCCGCACCCTCCGGAGCGTGCAGCCGCCGTGCCGTTTT CAGGGCTGCAGCCTGCGCAGGGAAGACGAATC |
| mm4_exon_Elac2_chr11_64979221 |
CATCCAAAGACCCACTGCGACACCTGCGTACGCGGGAGAAGCGCGGCCCGGGTCCCGGGGGCCCGAACACCGTGTACCTG CAGG |
| mm4_intergenic_E130114P18Rik|Gm12676_chr4_97751384 |
CGTGCTTGAACCCCAATCCTCCCGCGGCCCAGGCCGTCCTCACCCTTCTACCCCTTCCCCTAAAGTCAGTAATGAGATTA AAGGGCTGAGGACAGTGTG |
| mm4_intron_Suv420h1_chr19_3768821 |
CACGGCCTGCAGTGTTGGCGGCGGCCGGAAGGGAGCGGTAGCGGTCGGCGAGGGCCGGGTCGCGGCCGCACGAGGGGAGC TGCTCGGCCGCTCGGGATTGGGGGACTGTGTTTTTCCTCTTCTCCAATGGGCG |
| mm4_exon_Nos1ap_chr1_170589733 |
GCCAGAAGCGGGACTAGACCGGGAGTCCGATCCCGAGCTCGGCGCGCACGGCCCGGGACGAGGAAGAAANNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNAGCAGGGTGCCTGTCACGGAGAC |
| mm4_intergenic_Cebpa|Slc7a10_chr7_35138629 |
TTTAGACATAAATGGTTCCCTTCACCCTCGTCCCAGGCCCCCCTCACCTAATTTCTCAAATGCTGGCTGCCAGCGGCTGT GGAGGATCCAGTCCAAGTGGGTGTGGGATGACTTGGGGAG |
| mm4_intergenic_Gnb4|Actl6a_chr3_32616645 |
GAGAGGGTCTGGGAACGCGCCGAGAGAGCGGCGCGCGTGGCTCGGGTCGGGGCTGGCGGGAGCGAGCGCGGTCGGGGCGG GGGGCGCTTGCAGGGGCTCGGGGAACCCAGAACGCGCTTGGCCTGGGTGGAGGCTGATTG |
| mm4_exon_Zfp131_chr13_119790756 |
ACAACGACTCCCCGGGTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACCCGGCCG GCACTCACTCAGGGAAGGAGGAGCGGCCCGGGGCCTGCGCGTCACTTAC |
| mm4_intron_Jam3_chr9_27155217 |
GAGCCGAAACCGCTATTCCACCGCGGCCCGGGCCGCTCTGTCCCCATCGGCACAGACCACACACTTCCTAGCGGCACTGA AGCGTCAACTCACCCCTGAAGAGCAGCAGCAGGAAGAAGTCAGGCA |
| mm4_intergenic_Dennd4b|Gatad2b_chr3_90293275 |
TGTCTGTACCGCAGTGTCTGCTCCGCCCGCCCGCCCTCCCCGACCCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNAGTGGGGTCTGGATCCCACTGTGC |
| mm4_intergenic_St6galnac5|St6galnac3_chr3_153027419 |
TGGAGCAGGCTATCCCAGTTACATCAACAAATGGAGAGGGCTGCCCGGGGCGGGGGTCTGCTTCTGCATTTTAAAACAGA TCATCATATTTATGACTGAATAGTAGAGTATCCAGACTCTTAAGGCCTCAAGT |
| mm4_exon_Gm26560/Drd5_chr5_38319529 |
AAAGGATCCCAAGCAGTGCAGGGTGAGCGCCCCTCGGGTCTGGGGACGGTCAGCTGCAGGGCCGGGAGCACCCTCTGGGG TGCCCGATGGGGCCTTCCGCGGGGCGCAGGGGCGAAGTTGGGATC |
| mm3_exon_Kdelr3_chr15_79516639 |
TGCTCCTGGTGAAGATCTGGAGGTCCAAGAGCTGCGCCGGTGANNNNNNNNNNNNNNNNNNNNNNACCGGACCATGGTGA GGACTTAGGCTCAGAGGGAAGCC |
| mm4_exon_Pde3b_chr7_114415943 |
GCCTGTGCCTTCTTCTTCCTCACCTGTTTCCTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCTGGTGGCT GCTGGCGCTGCCCGCCTGCTGCTACCTGGGCGACTTC |
| mm4_intron_Vkorc1l1_chr5_129942458 |
AGCTCCGGCCTGAGAGAGCGGGGCCCGGGCCGCGACCCGGACGGCGGTGACCGGGGGGCGGCGGCTGAGGGCAGGCCTCC GGGATGAGGTGGCACAGTGCA |
| mm4_intron_Dtx1_chr5_120711190 |
GGAGAACAGCGGGCATCATACCCCAGGCTGTCACCCGCAGTGGCCCGTGTTCGGTTGGAGCGCGTNNNNNNNNNNNNNNN NNNNNNTACCGGGGGCTGCGGTGTGGGATGCAGGGACGCGGGATGTGGAGATGCAGAA |
| mm4_exon_Gpr146_chr5_139380742 |
CCTCCTGCAAGCCCAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCGAGCGGTGAGTGGGGCCCGGGGAGGG GAGCCCCTAATGCTCTGTTCCA |
| mm2_intron_Lhx4_chr1_155741355 |
TCGAACTGCCGAAACCAAGAGTGTGACAGGGGGCTTCTGTACAGCCCTGCGACCCGGGACGCGCTCTCCAACTTCCCGCC AGCTGGGGTAAAGTTGT |
| mm4_exon_Hdac5_chr11_102206816 |
CGGAAGGGAGGGCAGAGGCGGTGGTGAGCCGGGCCCGGGCAGAGGCGGTGGTGAGCCGGGCCCGGGCAGGGCTGCTTGGG CCTCTCGCGGCCCCTGAGCCCCCCTGGCTCACCTTGAGGTGCTTCTGCAGCTGGACCTCG |
| mm4_exon_Dlgap3_chr4_127169310 |
CAGTGTCGGGTCCGGGAGCCCGCACTCGCTCCCGCAGCCGCANNNNNNNNNNNNNNNNNNNNNNNNACCATGTAACCCCG GCTGGAGCCCGACCGGGCCAGGTAAGCAACCGGGAGG |
| mm4_intron_Zp1_chr19_10919949 |
CATTTGGGGACACCTGCTCACTTGCATTGTTATTTCAGAACAGCAAACACCGAACNNNNNNNNNNNNNNNNNNNNNNNNN NNNNGGGCTGCCCTCACCCTTCTTTTTCCATGCACAGGGCAAGTGTGTGTGTGGGAAGGG |
| mm4_exon_Prokr2_chr2_132385305 |
GGTACTCACTTGCCTCGGTGCTTTCAGGGACGCACTCCCTGGCCGGTGGCGCCACCATAGGTGGGCGCGGCCCGGGCTCT GGGAACACTAGCTCTGGTTGAGGCTTGC |
| mm3_intron_Scn5a_chr9_119512904 |
AAAGGGAAGCAGACTCCAGCTCAGGGTGAGCACGGCCCGGGTGCTGGGGAACTCTTGGGTCTCCTGTCCCCTCTGGGTGT GGCCGAGAGCTGCCCGGTACCTTCAGTGAAGCAGTCCTCTGGG |
| mm4_intergenic_Rps6-ps2|mmu-mir-8110_chr8_88918804 |
GGTCGATTAAGTCACAGCAAGCATGCCTGGCATGACTGGGGTGGGGAGCGTGGCCCAGGTGGTGGAGGAAATGTGTACCA TCTTTGTACCATCAAACATCGTTTGTCACATAGCGATCACA |
| mm4_intron_Ppard_chr17_28232883 |
AAGTTTTGGCAGGAGCTGGGGGATTCTGCGGAGCCTGCGGGACGGCGGCAGCGGCGCGAGAGGCGGCCGGGACAGGTCCG TGCGGATCGGGAGAGGCCAGCGCGGCCCGGGCTGAGGTGGATGGTGGGGTCAGGG |
| mm4_intron_Smarcad1_chr6_65042997 |
GTGAGGACCTCGTGCGAGGGTCGGCTCGGGCTCGTGGGGTCGCGCGGGCCGGGTCGAGGGCGCGACGGGCATTTGGCCGG GACTGCAATCCGCCGTCCCCGTGTGGCCTAACAAAGCACAGC |
| mm4_intron_Trpv4_chr5_114650152 |
GTGCCTGGAATGGGGTAGACGGCAGAATGCAGAGAGTGCGGGGGGAGGGGGGCTACTGAGGACCATTAGTGAATAAGAAC AAGGACCAGGGGTGAGTGCGGGCTGGGTGGGGGCTCTACAGCAGGAGACAGACCCT |
| mm4_exon_Cbr1_chr16_93608063 |
CTCGTGACCTGTGTCGGAAATTCTCCGGGGACGTGGTGCTCGCGGCGCGGGACGAGGAGCGGGGCCAAACGGCAGTGCAA AAGCTGCAGGCGGAGGGCCTGAGCCCACGCTTCCACCAGCTGGACATCGACAA |
| mm4_exon_Stox2_chr8_47288355 |
GGGCTTCCAGCTTCCAGATTCCTGAGGGGGAAGGCACGTTGGGAGGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNAGACTTGCAGCGTGGAAGCAATGGGAGCGCAGACAATG |
| mm4_exon_Snrk_chr9_122166947 |
CCAGAGCAAGCCTAGCAGCGGTGGCGGTGTGGACAAGGCCAGCCCGGGTGAGCAGGGCACGGGTGGTGGCAGCCAGGGAG GCTCTGGTGGGACCCCGTCGGGTACAGCAGGGTCCTCAAG |
| mm4_intergenic_Acsbg1|Dnaja4_chr9_54691650 |
TAACTGTCTCCGGGAAGCAGCCTCGGTGGCAGGGCCAGGTCTCTCGGCTGGCTTCCGGGAGCGCGCGCCCGCCCCCTACC CGCGCTGCGCTCCCCGTTCTCCGTTAGGCGCGCTCGCACTGCTCGCTCCTCAAACTCCTT |
| mm4_exon_Tmem230_chr2_132247700 |
TAACTGTCTCCGGGAAGCAGCCTCGGTGGCAGGGCCAGGTCTCGCGGCTGGCTTCCGGGAGCGCGCGCCCGCCCCCTACC CGCGCTGCGCTCCCCGTTCTCCGTTAGGCGCGCTCGCAAGGCTCGCTCAA |
| mm4_intergenic_Gm25456|BC048562_chr9_108431846 |
CACCTCGAGTTCTCACCTCGCCACACGCGACAGCACACGCCTGCCTTCCTGCGGCTTCCTGGGCGCCGAAGAAAGCCCGC CGAGGTGAGGCCCCACCCCCGGGCTGCGCTCACCAATAAGAATGCCGATTCTTGACCCCG |
| mm4_intron_Ubqln4_chr3_88554086 |
GGCATACAGGCGGGAGAAGCGGGTAGGGGGCGCCCTGCCAGGGCTAGTCCGGGGAGCGTGGCGCGGGTAGGGGCCGGTTT GGCCGCGCTACCCGGGCTGGGGGCGTGGTGGGGGAGCTTGAGGAAGGGACAC |
| mm4_exon_Rrm2_chr12_24708444 | GACTCACCCTGGCTGACAAGGAGAACACGGTGAGGGCGGCCCAGGTGCCGGCTGGGTTGGATGTGCACTGG |
| mm4_intergenic_Zfp69|Smap2_chr4_120951722 |
CTCTAGCCCGGTTCGTGTCCCCGCGCCTCCACCCGGGACCCGCCCACCCCACGCTGCTCGCGTCAGCGCCCTGCCCGTGG CTCCGCCCACCCGGGTGCTGCGCGACCCAGATCCCGGAAGC |
| mm4_intergenic_Utp15|Ankra2_chr13_98263093 |
CAGGCGCAGAAGGTTTACGACCCAAAGAACTACAAGAAAGCGCCCTGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN TCTCCAATGACCCGGAACTGAGGCGATCCATGGTTCTCCGAGAGGATGGGCTAC |
| mm4_intron_Myh14_chr7_44639141 |
AGGGTGGTGAAGGATGGGATGGTGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGAGGGAGCCAGAT TAGGCGAGAAGGACCGCAAGACCACGGTGCACACCTGGAAGATCTCAAAGCCCGCGAT |
| mm4_intergenic_Rps2-ps6|Tsc22d1_chr14_76414152 |
ACCGCTCTGCACAAAAGGTACCACTGCTGTGGGGGAAGACACCCGATTCTATCGGGGCATCCCCGGAAGGGGCGAGGGCG GTGCGGGTCGAGGGGGAGGCCAGGTTGCTGCTTGGAGAAGGAGG |
| mm4_intron_Bves_chr10_45336061 |
CGCTGACACTGCTGTGAGAGCAGGGCGAGCGGGTCCCGGGTGGGGGAGCAGTGGGAGCCACGGGTCCTCGCCACCCGCCC GCGTCCTAGAGAAACGGGTGGCTG |
| mm4_exon_Clk3_chr9_57765812 |
CCTTGAACACCTCAGAGCGTCTTGCTGTCAGTCTTGCTAGGAAACCCGCGCCACACAAAAGCCCCGAGCCGCGCCGCGCT CCCGCTCTGCCACTCGAGTCACTCAG |
| mm4_exon_Rhou_chr8_123654215 |
CCTACGGCCTTCGACAACTTCTCGGGTGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCGACCGGTCTGGCTCCGGGATTC |
| mm4_intron_Cisd2_chr3_135423180 |
TCACGTGCCAAGGCCTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTCTCCACCGCTGCCCCCACGGGGGCGG CGCGCGAGGATGGGACGGCGGCGGGAGGGTTACCTGTGAGGCG |
| mm4_intron_Ptpn2_chr18_67724318 |
TCGGTGCTTCAGGTCTAAGCCTCACAGCGGTGAGGACTGTCCGCGCAGGCGCGCCCCTGACGCGGCCCGCGCTCGCCACT TCCGCCCGAGCGGGAGACGGGGGAGGTGACACGGAGC |
| mm4_intron_Fam154b_chr7_82648333 |
GGTTCAGGCAAGGGAGTCCTTAGGGGGTGTGCGCGGCGGGGCTCGCGGCTCTGCGGACGCCACACCGGGCGGACGTTTCT TACCCGCAGGTGCAAATCTGGCACAGACACCAGTTCCTCATGCTCTGC |
| mm4_intergenic_Kcnma1|Gm6158_chr14_24004404 |
ATGAGGGAGGGTGGCTCCCACTCCCCGACCCCGGCCCCGATCGCCCCCGGGGCTGCGCGGAGCACCGAGAGCCCANNNNN NNNNNNNNNNNNNNNNNNNNNNNNNTCTGTGCCTCCCGAAGCTC |
| mm4_intergenic_Gpr25/Gm26781|Camsap2_chr1_136267527 |
AGATGCTCCTGTCTGCTGTGGGGCATTTTCCTCGACCCCAGCAGCGTTCACCCGCTTATTAGCCAGGGGGAGGGAAAGCA TCATAGTTTATGGATATTCCTCCCTCTGCTGATACACT |
| mm4_exon_Otud5_chrX_7842484 |
CAGGCTTCTCCACCTCCTTGTGGGGGCCCCGGTGGCCCCGGCGGCGGTCCTGGTGACGCTCTTGGTGCGACAACTGCGGG GGTGGGCGCGGCAGGGGTGGTGGTGGGCGTGGGTGGTACCGTGGG |
| mm4_exon_Gm13111_chr4_154637649 |
CTCCAAGCTCTGCCACCGCCCGGGACGCCGAGTGGCGCTGGAGCCGTCGGTCCTCAGCCGGGCGCGGCGGCTGAGGCTAG CGCGCCCCGCGTCGGGGGGCGGCCGTGCGAAGGCCGGCATCTTGTCCTGGGCGTCTC |
| mm4_exon_Dgcr6_chr16_18066655 |
CAGCACTACAGAGCCTGGTCAAGGAGCTGCCCAGGTGCGCGGCGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNCGCTCACCCGGCGATCTACCCGCAGCTCCTTTCAG |
| mm4_exon_Agps_chr2_75832334 |
TTTCACCCGAGCCCAGCGGCCTCGGGCGGCGGCACAAGGCGGAAGCCATGGCGGAGGCGGCGGCGGGCGAGGCGGGCGCG AGCGAGCGGGACCCAGANNNNNNNNNNNNNNNNNNNNNNNTGCGGGTTCTGTCGGGCCAT |
| mm3_exon_Tuba3a_chr6_125285945 |
CTCAGAGGAGACGGAGGTCAACCTCCCGTGGGGCCGACCCCCCCAGAGGCCCCGAGGCCAACCACCCCGGCCTGGGACTG CTCCCACCACCCCGGCCGCTCTCACCATGCTGAACTCCGGAGACCC |
| mm3_exon_Lin7c_chr2_109890920 |
CTCCGAAGAGAAGATGGCGGCGCTGGGCGAACCTGTCCGGCTGGAAAGGGGTGAGTGCGGCGGGGGTCGCGGCCGCGGTG GGTGGGGCGGGTCGAGACTACGAACCGCTC |