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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| mm0_exon_Pop5_chr5_115238095_F | TCGTCGGCAGCGTCTGCGGTTCAAGCACAGGTAA | 60.1 | 1.00 |
| mm0_exon_Pop5_chr5_115238095_R | GTCTCGTGGGCTCGGCTCGCACAGAAGGTACCTGC | 60.7 | 1.00 |
| mm2_intron_Lhx4_chr1_155741354_F | TCGTCGGCAGCGTCTCGAACTGCCGAAACCAAGA | 59.8 | 0.68 |
| mm2_intron_Lhx4_chr1_155741354_R | GTCTCGTGGGCTCGGACAACTTTACCCCAGCTGGC | 60.5 | 0.68 |
| mm4_intergenic_Cldn23|Gm22030_chr8_35847617_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Cldn23|Gm22030_chr8_35847617_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Gm12604|Mllt3_chr4_87598003_F | TCGTCGGCAGCGTCGCAGGAGTTCTGGAACCACA | 59.8 | 0.35 |
| mm4_intergenic_Gm12604|Mllt3_chr4_87598003_R | GTCTCGTGGGCTCGGGTGGTTACAGTCCACCATAGGT | 59.4 | 0.35 |
| mm4_intron_Gm5656_chr12_103700633_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intron_Gm5656_chr12_103700633_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_exon_Elac2_chr11_64979222_F | TCGTCGGCAGCGTCCATCCAAAGACCCACTGCGA | 60.3 | 0.25 |
| mm4_exon_Elac2_chr11_64979222_R | GTCTCGTGGGCTCGGCCTGCAGGTACACGGTGTTC | 60.9 | 0.25 |
| mm4_intergenic_E130114P18Rik|Gm12676_chr4_97751383_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_E130114P18Rik|Gm12676_chr4_97751383_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_exon_ATF7/Atf7_chr15_102625272_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_exon_ATF7/Atf7_chr15_102625272_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Six2|Srbd1_chr17_85688435_F | TCGTCGGCAGCGTCCTGTCAGTCACCGGTAACCC | 60.0 | 0.22 |
| mm4_intergenic_Six2|Srbd1_chr17_85688435_R | GTCTCGTGGGCTCGGCAACCTCCGCTCTGTGCA | 59.9 | 0.22 |
| mm4_exon_Gm17641/1110032F04Rik_chr3_68869578_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_exon_Gm17641/1110032F04Rik_chr3_68869578_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Bnip2_chr9_69989689_F | TCGTCGGCAGCGTCGAGGGGCAAAGAGGACGAG | 59.7 | 0.20 |
| mm4_intron_Bnip2_chr9_69989689_R | GTCTCGTGGGCTCGGGATCCTCCCGCAGCCCTC | 61.9 | 0.20 |
| mm4_intron_Midn_chr10_80148729_F | TCGTCGGCAGCGTCAATTCTTGGCGCACCCCTT | 60.2 | 0.18 |
| mm4_intron_Midn_chr10_80148729_R | GTCTCGTGGGCTCGGCAGCGGTGAGTCAGCAGC | 61.1 | 0.18 |
| mm4_intron_RPL24/RP24-288C12.6_chr16_55966638_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_RPL24/RP24-288C12.6_chr16_55966638_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Rybp_chr6_100286881_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Rybp_chr6_100286881_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Sbno2_chr10_80060518_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Sbno2_chr10_80060518_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intron_Ckap4_chr10_84533900_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intron_Ckap4_chr10_84533900_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Wnt8b_chr19_44511849_F | TCGTCGGCAGCGTCCGAGGGCCGAGAGTGTCT | 60.7 | 0.15 |
| mm4_exon_Wnt8b_chr19_44511849_R | GTCTCGTGGGCTCGGTTGCAGCTGGACACTGTCTC | 60.2 | 0.15 |
| mm4_exon_Dusp28_chr1_92907188_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Dusp28_chr1_92907188_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Sh3gl2_chr4_85205445_F | TCGTCGGCAGCGTCCTGACGTCAGGGTGTCTCTC | 59.4 | 0.12 |
| mm4_exon_Sh3gl2_chr4_85205445_R | GTCTCGTGGGCTCGGCAGATGCAGATGCGGAGAGG | 60.6 | 0.12 |
| mm4_intron_Ceacam10_chr7_24777693_F | TCGTCGGCAGCGTCACACTAGGCTCTGGGACACT | 59.8 | 0.11 |
| mm4_intron_Ceacam10_chr7_24777693_R | GTCTCGTGGGCTCGGAGGCTAGGTGGATTTTGGCT | 58.9 | 0.11 |
| mm3_exon_Eif4e2_chr1_87214170_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_exon_Eif4e2_chr1_87214170_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Tmem245_chr4_56947334_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Tmem245_chr4_56947334_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_St3gal5|Atoh8_chr6_72189028_F | TCGTCGGCAGCGTCAAGGAGCCCTTACACACAGC | 59.9 | 0.06 |
| mm4_intergenic_St3gal5|Atoh8_chr6_72189028_R | GTCTCGTGGGCTCGGCCCTCAAATTGGGGCTGGAG | 60.6 | 0.06 |
| mm4_exon_Mycn_chr12_12939731_F | TCGTCGGCAGCGTCCGGGGCAGTAGCTGGAAC | 60.1 | 0.06 |
| mm4_exon_Mycn_chr12_12939731_R | GTCTCGTGGGCTCGGCTTCCCCTTCCCGGTGAAC | 60.0 | 0.06 |
| mm4_exon_Brap_chr5_121660949_F | TCGTCGGCAGCGTCCTGCTCGACGTCGCCCTC | 62.8 | 0.05 |
| mm4_exon_Brap_chr5_121660949_R | GTCTCGTGGGCTCGGTCTGTCTGACTGCCAGCTTG | 59.9 | 0.05 |
| mm4_intron_Phip_chr9_82974985_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Phip_chr9_82974985_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Rbm19_chr5_120182747_F | TCGTCGGCAGCGTCACAATGAGTTGCCGATGGGT | 59.9 | 0.03 |
| mm4_intron_Rbm19_chr5_120182747_R | GTCTCGTGGGCTCGGGGGACAACAAGGGACACTGT | 59.8 | 0.03 |
| mm4_exon_Pdx1_chr5_147270461_F | TCGTCGGCAGCGTCTACAAGCTCGCTGGGATCAC | 59.8 | 0.03 |
| mm4_exon_Pdx1_chr5_147270461_R | GTCTCGTGGGCTCGGCTGAGCTGGAAGGTGGTGG | 60.0 | 0.03 |
| mm4_intron_Ykt6_chr11_5956008_F | TCGTCGGCAGCGTCGCGTACGACGTGTCTTCCTT | 60.3 | 0.03 |
| mm4_intron_Ykt6_chr11_5956008_R | GTCTCGTGGGCTCGGAGACTCGACTCCCTGGACTC | 60.0 | 0.03 |
| mm4_exon_Prkcd_chr14_30605792_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Prkcd_chr14_30605792_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Pex7_chr10_19907435_F | TCGTCGGCAGCGTCGACCTAGGAGGGAGGGCC | 60.1 | 0.03 |
| mm4_intron_Pex7_chr10_19907435_R | GTCTCGTGGGCTCGGCAGCACTACGGCATCGCG | 61.6 | 0.03 |
| mm4_intergenic_Rerg|Ptpro_chr6_137193370_F | TCGTCGGCAGCGTCGTCTTGTCTCCACTCTGGCC | 60.0 | 0.03 |
| mm4_intergenic_Rerg|Ptpro_chr6_137193370_R | GTCTCGTGGGCTCGGAGCACCTCTTACCCTGCTTG | 59.6 | 0.03 |
| mm4_intron_Tbc1d9b_chr11_50131556_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Tbc1d9b_chr11_50131556_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Bnc2_chr4_84675189_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Bnc2_chr4_84675189_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cmas_chr6_142757038_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cmas_chr6_142757038_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Atoh7_chr10_63100241_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Atoh7_chr10_63100241_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Scn5a_chr9_119512905_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Scn5a_chr9_119512905_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Arhgap21_chr2_20968225_F | TCGTCGGCAGCGTCAAAAGCCAGCTCGGACCAG | 60.3 | 0.01 |
| mm4_intron_Arhgap21_chr2_20968225_R | GTCTCGTGGGCTCGGAGGAAAACAGCCGTCTCCTT | 59.2 | 0.01 |
| mm4_intron_Zbtb7c_chr18_75820512_F | TCGTCGGCAGCGTCCGTTGCTAGGAGGCGGAAG | 60.5 | 0.01 |
| mm4_intron_Zbtb7c_chr18_75820512_R | GTCTCGTGGGCTCGGGAGCCCCTGAATAGCCACC | 59.8 | 0.01 |
| mm4_intron_Tbkbp1_chr11_97151350_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Tbkbp1_chr11_97151350_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Cdk5rap3_chr11_96911427_F | TCGTCGGCAGCGTCCTGGTCTCAGGAAAGCCAGG | 60.0 | 0.01 |
| mm4_intron_Cdk5rap3_chr11_96911427_R | GTCTCGTGGGCTCGGGGAGCTGTTTCCCATCCACA | 59.9 | 0.01 |
| mm3_exon_Mocs3_chr2_168230778_F | TCGTCGGCAGCGTCGAGGAAGAGCTAGCTTCGCT | 59.2 | 0.01 |
| mm3_exon_Mocs3_chr2_168230778_R | GTCTCGTGGGCTCGGCTGTAGCGGAGGATCTCGTC | 59.4 | 0.01 |
| mm4_intron_Slc6a11_chr6_114131517_F | TCGTCGGCAGCGTCAGATCATCGGACTGGGCAAC | 59.8 | 0.00 |
| mm4_intron_Slc6a11_chr6_114131517_R | GTCTCGTGGGCTCGGACGAGAGGTGTCACCCCA | 59.8 | 0.00 |
| mm4_intron_Triobp_chr15_78982932_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Triobp_chr15_78982932_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hivep1_chr13_42052258_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hivep1_chr13_42052258_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Lhcgr|Fshr_chr17_88823424_F | TCGTCGGCAGCGTCCGGTTTGGGAGTAGTGTGCT | 59.9 | 0.00 |
| mm4_intergenic_Lhcgr|Fshr_chr17_88823424_R | GTCTCGTGGGCTCGGGCATGACAGAGATGCAAGCG | 59.9 | 0.00 |
| mm4_intron_Usp12_chr5_146794580_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Usp12_chr5_146794580_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tuba3a_chr6_125285944_F | TCGTCGGCAGCGTCCTCAGAGGAGACGGAGGTCA | 60.0 | 0.00 |
| mm4_intron_Tuba3a_chr6_125285944_R | GTCTCGTGGGCTCGGGGTCTCCGGAGTTCAGCATG | 60.4 | 0.00 |
| mm3_intergenic_Ccdc134|Srebf2_chr15_82147202_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Ccdc134|Srebf2_chr15_82147202_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Klf4_chr4_55532317_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Klf4_chr4_55532317_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rb1|Itm2b_chr14_73326134_F | TCGTCGGCAGCGTCAAAACAAACGTGCCGGGC | 59.8 | 0.00 |
| mm4_intergenic_Rb1|Itm2b_chr14_73326134_R | GTCTCGTGGGCTCGGGCCCATCTTCCCTTGTCTCC | 60.1 | 0.00 |
| mm4_intergenic_Gm13501|Gm13503_chr2_53485261_F | TCGTCGGCAGCGTCGCTCCAGCCCCAGAATCTC | 59.8 | 0.00 |
| mm4_intergenic_Gm13501|Gm13503_chr2_53485261_R | GTCTCGTGGGCTCGGAGTCAAATGAGGCAAGGGACA | 59.5 | 0.00 |
| mm3_intergenic_Mir181c|Gm26887_chr8_84183774_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Mir181c|Gm26887_chr8_84183774_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Slc4a11_chr2_130697290_F | TCGTCGGCAGCGTCATCAGGACTCCGTGGTTCTG | 59.1 | 0.00 |
| mm4_intron_Slc4a11_chr2_130697290_R | GTCTCGTGGGCTCGGGGATCCAGAACAGACCTCGC | 60.1 | 0.00 |
| mm4_intron_Ndufa11_chr17_56717942_F | TCGTCGGCAGCGTCAAGACCTACATCACCACGGC | 59.7 | 0.00 |
| mm4_intron_Ndufa11_chr17_56717942_R | GTCTCGTGGGCTCGGCCCTCTGACCTTTGCTTCCA | 59.5 | 0.00 |
| mm4_intergenic_Gm2895|St3gal1_chr15_67069125_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm2895|St3gal1_chr15_67069125_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25696|Atp11b_chr3_35753870_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25696|Atp11b_chr3_35753870_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Vps37b_chr5_124032177_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Vps37b_chr5_124032177_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Car11_chr7_45699975_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Car11_chr7_45699975_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Dym|Gm20544_chr18_75343040_F | TCGTCGGCAGCGTCCTCCCGTGGATTCAGGCC | 59.8 | 0.00 |
| mm3_intergenic_Dym|Gm20544_chr18_75343040_R | GTCTCGTGGGCTCGGGCACCAAGCCAGACACACT | 60.5 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm0_exon_Pop5_chr5_115238095 |
TGCGGTTCAAGCACAGGTAAGCAGCGCTGTCCCCGCGCCGTCCCCGCGACCCGGGCCGCGCTCACCCCGCTGTCGCCGCA GGTACCTTCTGTGCGAG |
| mm2_intron_Lhx4_chr1_155741354 |
TCGAACTGCCGAAACCAAGAGTGTGACAGGGGGCTTCTGTACAGCCCTGCGACCCGGGACGCGCTCTCCAACTTCCCGCC AGCTGGGGTAAAGTTGT |
| mm4_intergenic_Gm12604|Mllt3_chr4_87598003 |
GCAGGAGTTCTGGAACCACAGCTGTGAGAGCGTCCTGGGTCTCAGGATGACAAAAAGAAACCATGTGACTAAGACACTTT GCCAACCAATTACCTATGGTGGACTGTAACCAC |
| mm4_exon_Elac2_chr11_64979222 |
CATCCAAAGACCCACTGCGACACCTGCGTACGCGGGAGAAGCGCGGCCCGGGTCCCGGGGGCCCGAACACCGTGTACCTG CAGG |
| mm4_intergenic_Six2|Srbd1_chr17_85688435 |
CTGTCAGTCACCGGTAACCCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGGCCAGCTCCGCTGACAGA TCGCCCCGGCTCTGCACAGAGCGGAGGTTG |
| mm4_intron_Bnip2_chr9_69989689 |
GAGGGGCAAAGAGGACGAGGGGGCCGCGGCTTCCTCCGGGGTGAGCACGGCTTGGGTAGCGGGCCGGGCTACGGCACAGC CGGAGGGCTGCGGGAGGATC |
| mm4_intron_Midn_chr10_80148729 |
AATTCTTGGCGCACCCCTTCCCCAGCCAAGACGCACGGCACTCCTCCCCCGGGACTCGGGCAGCGCTCCCGGGCGGGGAC TGCAGAGGGCGCGCCGGGGCTGCTGACTCACCGCTG |
| mm4_exon_Wnt8b_chr19_44511849 |
CGAGGGCCGAGAGTGTCTGCGGCGCGGGCGCGCCCTGGGTCGCTGGGAGCGCCGCAGTTGTCGCCGGCTGTGCGGGGACT GCGGGCTAGCGGTGGAGGAGCGCCGCGCCGAGACAGTGTCCAGCTGCAA |
| mm4_exon_Sh3gl2_chr4_85205445 |
CTGACGTCAGGGTGTCTCTCCGCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCAGCTCCCGCGGTCGCACGACC AGCGGCGGCCCGGCGACCCCAGCCGCCTCTCCGCATCTGCATCTG |
| mm4_intron_Ceacam10_chr7_24777693 |
ACACTAGGCTCTGGGACACTGATTTGTACACGGGTATGTGAGCGTGTTCCGGGTAGCAGGGACTAAGCCAAAATCCACCT AGCCT |
| mm4_intergenic_St3gal5|Atoh8_chr6_72189028 |
AAGGAGCCCTTACACACAGCGCAAATGTGTCCCTCGCTGCCTGGATTCTCCTGGAACCCGGGCTGCGCGCACAGACAGAA GTTGTGTACACAGGATTTTAGTATTTCCTCTCCGGCTCCAGCCCCAATTTGAGGG |
| mm4_exon_Mycn_chr12_12939731 |
CGGGGCAGTAGCTGGAACAGACACACTAGTGACCGCAGCGCTGGTCGCCGGGGCGCTAGTGGGGGCAGCGGGCACCGACG CCGACTCTCGCTTGTTCACCGGGAAGGGGAAG |
| mm4_exon_Brap_chr5_121660949 |
CTGCTCGACGTCGCCCTCGCTGCGAGAAAGCGCGGCCCAGGGCGCGGGGGCGATGCTGTGCGCCTTTCCCAAGCTGGCAG TCAGACAGA |
| mm4_intron_Rbm19_chr5_120182747 |
ACAATGAGTTGCCGATGGGTGCTAGAACCCACACCAGGTGTGCCAGGCCCGGGTGGCAGGGCCTTTGCAGGCTAGGCCAA GTTTCAGTGCTCGAGAAGATGAAGCACACAGTGTCCCTTGTTGTCCC |
| mm4_exon_Pdx1_chr5_147270461 |
TACAAGCTCGCTGGGATCACTGGAGCAGGGAAGTCCTCCGGACATCTCCCCATACGAAGTGCCCCCGCTCGCCTCCGACG ACCCGGCTGGCGCTCACCTCCACCACCACCTTCCAGCTCAG |
| mm4_intron_Ykt6_chr11_5956008 |
GCGTACGACGTGTCTTCCTTTAGTTTCTTCCAGAGGTCCAGGTAAGTGGGCCGGGCCCGGGTGGCTGGACCCGGAGTCCA GGGAGTCGAGTCT |
| mm4_intron_Pex7_chr10_19907435 |
GACCTAGGAGGGAGGGCCGAGCTCTGTCGCCCCCTGGCGGTGGCGGCGGCACCGCGCGCTCAGCGGCGCGTCCTACGCCC GCCCCGGAGGCGCGGCCCGCGCTCACCCGCGATGCCGTAGTGCTG |
| mm4_intergenic_Rerg|Ptpro_chr6_137193370 |
GTCTTGTCTCCACTCTGGCCGCTGCAGGTCTGCAGTGAGCTCTGCCTGTGTCGCTGGCTTCCCAGTCTCAGTGTGTTCTG AACATCACTGGGGCAAGCAGGGTAAGAGGTGCT |
| mm4_intron_Arhgap21_chr2_20968225 |
AAAAGCCAGCTCGGACCAGGGCGGGCGTGGGCTCGGCGGCGTCTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGGAGACGGCTGTTTTCCT |
| mm4_intron_Zbtb7c_chr18_75820512 |
CGTTGCTAGGAGGCGGAAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCATCGCTGGGCAGGAGGCG GGGGGCTGCGGTGGCTATTCAGGGGCTC |
| mm4_intron_Cdk5rap3_chr11_96911427 |
CTGGTCTCAGGAAAGCCAGGAAAATCAAAGGGCCTTTGGGTCCGCCTACCCGAGCCTCGCTCACCCAGCTTCCCTTACTC TTCACTATGGGAAGTCAGGTCAGGGCTCGGGCTCCGTGTGGATGGGAAACAGCTCC |
| mm3_exon_Mocs3_chr2_168230778 |
GAGGAAGAGCTAGCTTCGCTCAAGCGGAGGCTGGCCGCGGCCCTGACAGCCGAGCCGGAGCCCGAGCGTCCGCTCCGGGT NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTCGCGGGACGAGATCCTCCGCTACAG |
| mm4_intron_Slc6a11_chr6_114131517 |
AGATCATCGGACTGGGCAACGTGTGGCGCTTTCCCTACCTGTGCTACAAAAACGGCGGAGGTGAGCGCGGCGGGGATGGC GGGGACGCGGGGCGACACCTGGGGTGGGGTGACACCTCTCGT |
| mm4_intergenic_Lhcgr|Fshr_chr17_88823424 |
CGGTTTGGGAGTAGTGTGCTACAGAAATAAACCTGCGACACGACCCGCGATCACTACTCACATGTGTTTCACCGCTTGCA TCTCTGTCATGC |
| mm4_intron_Tuba3a_chr6_125285944 |
CTCAGAGGAGACGGAGGTCAACCTCCCGTGGGGCCGACCCCCCCAGAGGCCCCGAGGCCAACCACCCCGGCCTGGGACTG CTCCCACCACCCCGGCCGCTCTCACCATGCTGAACTCCGGAGACC |
| mm4_intergenic_Rb1|Itm2b_chr14_73326134 |
AAAACAAACGTGCCGGGCCAGTGGGAGGTGGGCTCGGCCCGGGTGGGCGGTCCAGGGTGGGGCCATTGGTGGCCTTCTGG AGACAAGGGAAGATGGGC |
| mm4_intergenic_Gm13501|Gm13503_chr2_53485261 |
GCTCCAGCCCCAGAATCTCCCCGCCCCCCGGCCCCCGCTCACTCCACTCCTCCATTTCTTCTTCATTTCCTAGTTTTTGA CATGGTCTAAAAAATTAGCTGAGGTGTCCTTAAACTTTTGTCCCTTGCCTCATTTGACT |
| mm4_intron_Slc4a11_chr2_130697290 |
ATCAGGACTCCGTGGTTCTGGGCCGGCTCGCAGCTGGAGCCGGCGACACTCCTGCGAGCCGCGCCGCGCTCCTAACCGTG CGGCGTGAGGCTGCTTCCAGCCAAAGTCGCGCCCTCACCCGCGAGGTCTGTTCTGGATCC |
| mm4_intron_Ndufa11_chr17_56717942 |
AAGACCTACATCACCACGGCCTTAGGCGGCATTTGCGGTGAGCGCGGGCCGGTGGGCGGGCGGAGGGCGGAGGTCTGGAA GCAAAGGTCAGAGGG |
| mm3_intergenic_Dym|Gm20544_chr18_75343040 |
CTCCCGTGGATTCAGGCCGCGCGCTCCTCGCTCCTGCGAACCCGGCCGCGCTCAAAGCTCCCCCTCCTCCACCTCTCGCC CTTGCCTCGCGGCTGAGTCACCGGCCTCTGGTCCCCAGTGTGTCTGGCTTGGTGC |